htslib 0.0.5 → 0.0.6
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- checksums.yaml +4 -4
- data/README.md +4 -3
- data/lib/hts/bam/cigar.rb +2 -0
- data/lib/hts/bam/flag.rb +6 -2
- data/lib/hts/bam/header.rb +5 -1
- data/lib/hts/bam/record.rb +10 -6
- data/lib/hts/bcf/format.rb +2 -1
- data/lib/hts/bcf/header.rb +11 -4
- data/lib/hts/bcf/info.rb +1 -0
- data/lib/hts/bcf/record.rb +6 -3
- data/lib/hts/bcf.rb +1 -1
- data/lib/hts/ffi_ext/struct.rb +1 -1
- data/lib/hts/libhts/hts.rb +6 -0
- data/lib/hts/libhts/sam.rb +2 -590
- data/lib/hts/libhts/sam_funcs.rb +595 -0
- data/lib/hts/libhts/vcf.rb +32 -439
- data/lib/hts/libhts/vcf_funcs.rb +430 -0
- data/lib/hts/version.rb +1 -1
- metadata +8 -6
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# frozen_string_literal: true
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module HTS
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module LibHTS
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# Generates a new unpopulated header structure.
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attach_function \
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:sam_hdr_init,
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[],
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SamHdr.by_ref
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# Read the header from a BAM compressed file.
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attach_function \
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:bam_hdr_read,
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[BGZF],
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SamHdr.by_ref
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# Writes the header to a BAM file.
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attach_function \
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:bam_hdr_write,
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[BGZF, SamHdr],
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:int
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# Frees the resources associated with a header.
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attach_function \
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:sam_hdr_destroy,
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[SamHdr],
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:void
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# Duplicate a header structure.
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attach_function \
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:sam_hdr_dup,
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[SamHdr],
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SamHdr.by_ref
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# Create a header from existing text.
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attach_function \
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:sam_hdr_parse,
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%i[size_t string],
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SamHdr.by_ref
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# Read a header from a SAM, BAM or CRAM file.
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attach_function \
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:sam_hdr_read,
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[SamFile],
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SamHdr.by_ref
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# Write a header to a SAM, BAM or CRAM file.
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attach_function \
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:sam_hdr_write,
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[SamFile, SamHdr],
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:int
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# Returns the current length of the header text.
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attach_function \
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:sam_hdr_length,
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[SamHdr],
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:size_t
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# Returns the text representation of the header.
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attach_function \
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:sam_hdr_str,
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[SamHdr],
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:string
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# Returns the number of references in the header.
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attach_function \
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:sam_hdr_nref,
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[SamHdr],
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:int
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# Add formatted lines to an existing header.
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attach_function \
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:sam_hdr_add_lines,
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[SamHdr, :string, :size_t],
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:int
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# Adds a single line to an existing header.
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attach_function \
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:sam_hdr_add_line,
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[SamHdr, :string, :varargs],
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:int
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# Returns a complete line of formatted text for a given type and ID.
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attach_function \
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:sam_hdr_find_line_id,
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[SamHdr, :string, :string, :string, KString],
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:int
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# Returns a complete line of formatted text for a given type and index.
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attach_function \
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:sam_hdr_find_line_pos,
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[SamHdr, :string, :int, KString],
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:int
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# Remove a line with given type / id from a header
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attach_function \
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:sam_hdr_remove_line_id,
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[SamHdr, :string, :string, :string],
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:int
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# Remove nth line of a given type from a header
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attach_function \
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:sam_hdr_remove_line_pos,
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[SamHdr, :string, :int],
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:int
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# Add or update tag key,value pairs in a header line.
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attach_function \
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:sam_hdr_update_line,
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[SamHdr, :string, :string, :string, :varargs],
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:int
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# Remove all lines of a given type from a header, except the one matching an ID
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attach_function \
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:sam_hdr_remove_except,
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[SamHdr, :string, :string, :string],
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:int
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# Remove header lines of a given type, except those in a given ID set
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attach_function \
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:sam_hdr_remove_lines,
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[SamHdr, :string, :string, :pointer],
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:int
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# Count the number of lines for a given header type
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attach_function \
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:sam_hdr_count_lines,
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[SamHdr, :string],
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:int
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# Index of the line for the types that have dedicated look-up tables (SQ, RG, PG)
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attach_function \
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:sam_hdr_line_index,
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[SamHdr, :string, :string],
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:int
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# Id key of the line for the types that have dedicated look-up tables (SQ, RG, PG)
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attach_function \
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:sam_hdr_line_name,
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[SamHdr, :string, :int],
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:string
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# Return the value associated with a key for a header line identified by ID_key:ID_val
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attach_function \
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:sam_hdr_find_tag_id,
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[SamHdr, :string, :string, :string, :string, KString],
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:int
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# Return the value associated with a key for a header line identified by position
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attach_function \
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:sam_hdr_find_tag_pos,
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[SamHdr, :string, :int, :string, KString],
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:int
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# Remove the key from the line identified by type, ID_key and ID_value.
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attach_function \
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:sam_hdr_remove_tag_id,
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[SamHdr, :string, :string, :string, :string],
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:int
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# Get the target id for a given reference sequence name
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attach_function \
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:sam_hdr_name2tid,
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[SamHdr, :string],
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:int
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# Get the reference sequence name from a target index
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attach_function \
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:sam_hdr_tid2name,
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[SamHdr, :int],
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:string
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# Get the reference sequence length from a target index
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attach_function \
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:sam_hdr_tid2len,
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[SamHdr, :int],
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:hts_pos_t
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# Generate a unique \@PG ID: value
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attach_function \
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:sam_hdr_pg_id,
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[SamHdr, :string],
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:string
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# Add an \@PG line.
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attach_function \
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:sam_hdr_add_pg,
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[SamHdr, :string, :varargs],
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:int
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# A function to help with construction of CL tags in @PG records.
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attach_function \
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:stringify_argv,
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%i[int pointer],
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:string
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# Increments the reference count on a header
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attach_function \
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:sam_hdr_incr_ref,
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[SamHdr],
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:void
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# Create a new bam1_t alignment structure
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attach_function \
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:bam_init1,
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[],
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Bam1.by_ref
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# Destroy a bam1_t structure
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attach_function \
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:bam_destroy1,
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[Bam1],
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:void
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# Read a BAM format alignment record
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attach_function \
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:bam_read1,
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[BGZF, Bam1],
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:int
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# Write a BAM format alignment record
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attach_function \
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:bam_write1,
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[BGZF, Bam1],
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:int
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# Copy alignment record data
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attach_function \
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:bam_copy1,
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[Bam1, Bam1],
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Bam1.by_ref
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# Create a duplicate alignment record
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attach_function \
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:bam_dup1,
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[Bam1],
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Bam1.by_ref
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# Calculate query length from CIGAR data
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attach_function \
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:bam_cigar2qlen,
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%i[int pointer],
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:int64
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# Calculate reference length from CIGAR data
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attach_function \
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:bam_cigar2rlen,
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%i[int pointer],
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:hts_pos_t
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# Calculate the rightmost base position of an alignment on the reference genome.
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attach_function \
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:bam_endpos,
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[Bam1],
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:hts_pos_t
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attach_function \
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:bam_str2flag,
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[:string],
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:int
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attach_function \
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:bam_flag2str,
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[:int],
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:string
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# Set the name of the query
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attach_function \
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:bam_set_qname,
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[Bam1, :string],
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:int
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# Initialise fp->idx for the current format type for SAM, BAM and CRAM types .
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attach_function \
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:sam_idx_init,
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[HtsFile, SamHdr, :int, :string],
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:int
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# Writes the index initialised with sam_idx_init to disk.
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attach_function \
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:sam_idx_save,
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[HtsFile],
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:int
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# Load a BAM (.csi or .bai) or CRAM (.crai) index file
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attach_function \
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:sam_index_load,
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[HtsFile, :string],
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HtsIdx.by_ref
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# Load a specific BAM (.csi or .bai) or CRAM (.crai) index file
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attach_function \
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:sam_index_load2,
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[HtsFile, :string, :string],
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HtsIdx.by_ref
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# Load or stream a BAM (.csi or .bai) or CRAM (.crai) index file
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attach_function \
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:sam_index_load3,
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[HtsFile, :string, :string, :int],
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HtsIdx.by_ref
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# Generate and save an index file
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attach_function \
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:sam_index_build,
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%i[string int],
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:int
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# Generate and save an index to a specific file
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attach_function \
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:sam_index_build2,
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%i[string string int],
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:int
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# Generate and save an index to a specific file
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attach_function \
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:sam_index_build3,
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%i[string string int int],
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:int
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# Create a BAM/CRAM iterator
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attach_function \
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:sam_itr_queryi,
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[HtsIdx, :int, :hts_pos_t, :hts_pos_t],
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HtsItr.by_ref
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# Create a SAM/BAM/CRAM iterator
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attach_function \
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:sam_itr_querys,
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[HtsIdx, SamHdr, :string],
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HtsItr.by_ref
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# Create a multi-region iterator
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attach_function \
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:sam_itr_regions,
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[HtsIdx, SamHdr, :pointer, :uint],
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HtsItr.by_ref
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# Create a multi-region iterator
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attach_function \
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:sam_itr_regarray,
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[HtsIdx, SamHdr, :pointer, :uint],
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HtsItr.by_ref
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# Get the next read from a SAM/BAM/CRAM iterator
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def self.sam_itr_next(htsfp, itr, r)
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# FIXME: check if htsfp is compressed BGZF
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hts_log_error("Null iterator") if itr.null?
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# FIXME: check multi
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hts_itr_next(htsfp[:fp][:bgzf], itr, r, htsfp)
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end
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attach_function \
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:sam_parse_region,
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[SamHdr, :string, :pointer, :pointer, :pointer, :int],
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:string
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# SAM I/O
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# macros (or alias)
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# sam_open
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# sam_open_format
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# sam_close
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attach_function \
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:sam_open_mode,
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%i[string string string],
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:int
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# A version of sam_open_mode that can handle ,key=value options.
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attach_function \
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:sam_open_mode_opts,
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%i[string string string],
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:string
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attach_function \
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:sam_hdr_change_HD,
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[SamHdr, :string, :string],
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:int
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attach_function \
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:sam_parse1,
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[KString, SamHdr, Bam1],
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:int
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+
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attach_function \
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:sam_format1,
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[SamHdr, Bam1, KString],
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:int
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391
|
+
# Read a record from a file
|
392
|
+
attach_function \
|
393
|
+
:sam_read1,
|
394
|
+
[HtsFile, SamHdr, Bam1],
|
395
|
+
:int
|
396
|
+
|
397
|
+
# Write a record to a file
|
398
|
+
attach_function \
|
399
|
+
:sam_write1,
|
400
|
+
[HtsFile, SamHdr, Bam1],
|
401
|
+
:int
|
402
|
+
|
403
|
+
# Return a pointer to an aux record
|
404
|
+
attach_function \
|
405
|
+
:bam_aux_get,
|
406
|
+
[Bam1, :string], # FIXME
|
407
|
+
:pointer
|
408
|
+
|
409
|
+
# Get an integer aux value
|
410
|
+
attach_function \
|
411
|
+
:bam_aux2i,
|
412
|
+
[:pointer],
|
413
|
+
:int64
|
414
|
+
|
415
|
+
# Get an integer aux value
|
416
|
+
attach_function \
|
417
|
+
:bam_aux2f,
|
418
|
+
[:pointer],
|
419
|
+
:double
|
420
|
+
|
421
|
+
# Get a character aux value
|
422
|
+
attach_function \
|
423
|
+
:bam_aux2A,
|
424
|
+
[:pointer],
|
425
|
+
:char
|
426
|
+
|
427
|
+
# Get a string aux value
|
428
|
+
attach_function \
|
429
|
+
:bam_aux2Z,
|
430
|
+
[:pointer],
|
431
|
+
:string
|
432
|
+
|
433
|
+
# Get the length of an array-type ('B') tag
|
434
|
+
attach_function \
|
435
|
+
:bam_auxB_len,
|
436
|
+
[:pointer],
|
437
|
+
:uint
|
438
|
+
|
439
|
+
# Get an integer value from an array-type tag
|
440
|
+
attach_function \
|
441
|
+
:bam_auxB2i,
|
442
|
+
%i[pointer uint],
|
443
|
+
:int64
|
444
|
+
|
445
|
+
# Get a floating-point value from an array-type tag
|
446
|
+
attach_function \
|
447
|
+
:bam_auxB2f,
|
448
|
+
%i[pointer uint],
|
449
|
+
:double
|
450
|
+
|
451
|
+
# Append tag data to a bam record
|
452
|
+
attach_function \
|
453
|
+
:bam_aux_append,
|
454
|
+
[Bam1, :string, :string, :int, :pointer],
|
455
|
+
:int
|
456
|
+
|
457
|
+
# Delete tag data from a bam record
|
458
|
+
attach_function \
|
459
|
+
:bam_aux_del,
|
460
|
+
[Bam1, :pointer],
|
461
|
+
:int
|
462
|
+
|
463
|
+
# Update or add a string-type tag
|
464
|
+
attach_function \
|
465
|
+
:bam_aux_update_str,
|
466
|
+
[Bam1, :string, :int, :string],
|
467
|
+
:int
|
468
|
+
|
469
|
+
# Update or add an integer tag
|
470
|
+
attach_function \
|
471
|
+
:bam_aux_update_int,
|
472
|
+
[Bam1, :string, :int64],
|
473
|
+
:int
|
474
|
+
|
475
|
+
# Update or add a floating-point tag
|
476
|
+
attach_function \
|
477
|
+
:bam_aux_update_float,
|
478
|
+
[Bam1, :string, :float],
|
479
|
+
:int
|
480
|
+
|
481
|
+
# Update or add an array tag
|
482
|
+
attach_function \
|
483
|
+
:bam_aux_update_array,
|
484
|
+
[Bam1, :string, :uint8, :uint32, :pointer],
|
485
|
+
:int
|
486
|
+
|
487
|
+
# sets an iterator over multiple
|
488
|
+
attach_function \
|
489
|
+
:bam_plp_init,
|
490
|
+
%i[bam_plp_auto_f pointer],
|
491
|
+
:bam_plp
|
492
|
+
|
493
|
+
attach_function \
|
494
|
+
:bam_plp_destroy,
|
495
|
+
[:bam_plp],
|
496
|
+
:void
|
497
|
+
|
498
|
+
attach_function \
|
499
|
+
:bam_plp_push,
|
500
|
+
[:bam_plp, Bam1],
|
501
|
+
:int
|
502
|
+
|
503
|
+
attach_function \
|
504
|
+
:bam_plp_next,
|
505
|
+
%i[bam_plp pointer pointer pointer],
|
506
|
+
BamPileup1.by_ref
|
507
|
+
|
508
|
+
attach_function \
|
509
|
+
:bam_plp_auto,
|
510
|
+
%i[bam_plp pointer pointer pointer],
|
511
|
+
BamPileup1.by_ref
|
512
|
+
|
513
|
+
attach_function \
|
514
|
+
:bam_plp64_next,
|
515
|
+
%i[bam_plp pointer pointer pointer],
|
516
|
+
BamPileup1.by_ref
|
517
|
+
|
518
|
+
attach_function \
|
519
|
+
:bam_plp64_auto,
|
520
|
+
%i[bam_plp pointer pointer pointer],
|
521
|
+
BamPileup1.by_ref
|
522
|
+
|
523
|
+
attach_function \
|
524
|
+
:bam_plp_set_maxcnt,
|
525
|
+
%i[bam_plp int],
|
526
|
+
:void
|
527
|
+
|
528
|
+
attach_function \
|
529
|
+
:bam_plp_reset,
|
530
|
+
[:bam_plp],
|
531
|
+
:void
|
532
|
+
|
533
|
+
# sets a callback to initialise any per-pileup1_t fields.
|
534
|
+
attach_function \
|
535
|
+
:bam_plp_insertion,
|
536
|
+
[BamPileup1, KString, :pointer],
|
537
|
+
:int
|
538
|
+
|
539
|
+
# sets a callback to initialise any per-pileup1_t fields.
|
540
|
+
# bam_plp_constructor
|
541
|
+
|
542
|
+
# bam_plp_destructor
|
543
|
+
|
544
|
+
# Get pileup padded insertion sequence
|
545
|
+
# bam_plp_insertion
|
546
|
+
|
547
|
+
attach_function \
|
548
|
+
:bam_mplp_init,
|
549
|
+
%i[int bam_plp_auto_f pointer],
|
550
|
+
:bam_mplp
|
551
|
+
|
552
|
+
attach_function \
|
553
|
+
:bam_mplp_init_overlaps,
|
554
|
+
[:bam_mplp],
|
555
|
+
:int
|
556
|
+
|
557
|
+
attach_function \
|
558
|
+
:bam_mplp_destroy,
|
559
|
+
[:bam_mplp],
|
560
|
+
:void
|
561
|
+
|
562
|
+
attach_function \
|
563
|
+
:bam_mplp_set_maxcnt,
|
564
|
+
%i[bam_mplp int],
|
565
|
+
:void
|
566
|
+
|
567
|
+
attach_function \
|
568
|
+
:bam_mplp_auto,
|
569
|
+
%i[bam_mplp pointer pointer pointer pointer], # BamPileup1T
|
570
|
+
:int
|
571
|
+
|
572
|
+
attach_function \
|
573
|
+
:bam_mplp64_auto,
|
574
|
+
%i[bam_mplp pointer pointer pointer pointer], # BamPileup1T
|
575
|
+
:int
|
576
|
+
|
577
|
+
attach_function \
|
578
|
+
:bam_mplp_reset,
|
579
|
+
[:bam_mplp],
|
580
|
+
:void
|
581
|
+
|
582
|
+
# bam_mplp_constructor
|
583
|
+
# bam_mplp_destructor
|
584
|
+
|
585
|
+
attach_function \
|
586
|
+
:sam_cap_mapq,
|
587
|
+
[Bam1, :string, :hts_pos_t, :int],
|
588
|
+
:int
|
589
|
+
|
590
|
+
attach_function \
|
591
|
+
:sam_prob_realn,
|
592
|
+
[Bam1, :string, :hts_pos_t, :int],
|
593
|
+
:int
|
594
|
+
end
|
595
|
+
end
|