htslib 0.0.5 → 0.0.6
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- checksums.yaml +4 -4
- data/README.md +4 -3
- data/lib/hts/bam/cigar.rb +2 -0
- data/lib/hts/bam/flag.rb +6 -2
- data/lib/hts/bam/header.rb +5 -1
- data/lib/hts/bam/record.rb +10 -6
- data/lib/hts/bcf/format.rb +2 -1
- data/lib/hts/bcf/header.rb +11 -4
- data/lib/hts/bcf/info.rb +1 -0
- data/lib/hts/bcf/record.rb +6 -3
- data/lib/hts/bcf.rb +1 -1
- data/lib/hts/ffi_ext/struct.rb +1 -1
- data/lib/hts/libhts/hts.rb +6 -0
- data/lib/hts/libhts/sam.rb +2 -590
- data/lib/hts/libhts/sam_funcs.rb +595 -0
- data/lib/hts/libhts/vcf.rb +32 -439
- data/lib/hts/libhts/vcf_funcs.rb +430 -0
- data/lib/hts/version.rb +1 -1
- metadata +8 -6
data/lib/hts/libhts/sam.rb
CHANGED
@@ -1,5 +1,7 @@
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1
1
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# frozen_string_literal: true
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2
2
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3
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+
require_relative "sam_funcs"
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4
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+
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3
5
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module HTS
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4
6
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module LibHTS
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5
7
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# constants
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@@ -96,595 +98,5 @@ module HTS
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96
98
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97
99
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# def bam_set_seqi(s, i, b)
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98
100
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end
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-
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100
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# Generates a new unpopulated header structure.
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attach_function \
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102
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:sam_hdr_init,
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[],
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104
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SamHdr.by_ref
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-
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106
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# Read the header from a BAM compressed file.
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107
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attach_function \
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108
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:bam_hdr_read,
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109
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[BGZF],
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110
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SamHdr.by_ref
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-
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# Writes the header to a BAM file.
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attach_function \
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:bam_hdr_write,
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[BGZF, SamHdr],
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:int
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-
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# Frees the resources associated with a header.
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attach_function \
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:sam_hdr_destroy,
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[SamHdr],
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122
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-
:void
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123
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-
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124
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# Duplicate a header structure.
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attach_function \
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:sam_hdr_dup,
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[SamHdr],
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128
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SamHdr.by_ref
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129
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-
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130
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# Create a header from existing text.
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attach_function \
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132
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:sam_hdr_parse,
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%i[size_t string],
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134
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SamHdr.by_ref
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135
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-
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# Read a header from a SAM, BAM or CRAM file.
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attach_function \
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:sam_hdr_read,
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[SamFile],
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SamHdr.by_ref
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141
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-
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142
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# Write a header to a SAM, BAM or CRAM file.
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attach_function \
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:sam_hdr_write,
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[SamFile, SamHdr],
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146
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:int
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-
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148
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# Returns the current length of the header text.
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attach_function \
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:sam_hdr_length,
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[SamHdr],
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:size_t
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-
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154
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# Returns the text representation of the header.
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attach_function \
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156
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:sam_hdr_str,
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[SamHdr],
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:string
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159
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-
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# Returns the number of references in the header.
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attach_function \
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:sam_hdr_nref,
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[SamHdr],
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:int
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-
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# Add formatted lines to an existing header.
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attach_function \
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:sam_hdr_add_lines,
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[SamHdr, :string, :size_t],
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:int
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-
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# Adds a single line to an existing header.
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attach_function \
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:sam_hdr_add_line,
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[SamHdr, :string, :varargs],
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:int
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-
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# Returns a complete line of formatted text for a given type and ID.
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attach_function \
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:sam_hdr_find_line_id,
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[SamHdr, :string, :string, :string, KString],
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:int
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# Returns a complete line of formatted text for a given type and index.
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attach_function \
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:sam_hdr_find_line_pos,
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[SamHdr, :string, :int, KString],
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:int
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-
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# Remove a line with given type / id from a header
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attach_function \
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:sam_hdr_remove_line_id,
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[SamHdr, :string, :string, :string],
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:int
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-
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# Remove nth line of a given type from a header
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attach_function \
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:sam_hdr_remove_line_pos,
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[SamHdr, :string, :int],
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:int
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-
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# Add or update tag key,value pairs in a header line.
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attach_function \
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:sam_hdr_update_line,
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[SamHdr, :string, :string, :string, :varargs],
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:int
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-
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# Remove all lines of a given type from a header, except the one matching an ID
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209
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attach_function \
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210
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:sam_hdr_remove_except,
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[SamHdr, :string, :string, :string],
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:int
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-
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# Remove header lines of a given type, except those in a given ID set
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215
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attach_function \
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216
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:sam_hdr_remove_lines,
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[SamHdr, :string, :string, :pointer],
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:int
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-
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# Count the number of lines for a given header type
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attach_function \
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222
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:sam_hdr_count_lines,
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[SamHdr, :string],
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:int
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-
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# Index of the line for the types that have dedicated look-up tables (SQ, RG, PG)
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attach_function \
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:sam_hdr_line_index,
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229
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[SamHdr, :string, :string],
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:int
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-
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# Id key of the line for the types that have dedicated look-up tables (SQ, RG, PG)
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233
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attach_function \
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234
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:sam_hdr_line_name,
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[SamHdr, :string, :int],
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:string
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-
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# Return the value associated with a key for a header line identified by ID_key:ID_val
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attach_function \
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:sam_hdr_find_tag_id,
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[SamHdr, :string, :string, :string, :string, KString],
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:int
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# Return the value associated with a key for a header line identified by position
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attach_function \
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:sam_hdr_find_tag_pos,
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[SamHdr, :string, :int, :string, KString],
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:int
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# Remove the key from the line identified by type, ID_key and ID_value.
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attach_function \
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:sam_hdr_remove_tag_id,
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[SamHdr, :string, :string, :string, :string],
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:int
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255
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-
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256
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# Get the target id for a given reference sequence name
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257
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attach_function \
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258
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:sam_hdr_name2tid,
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259
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[SamHdr, :string],
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:int
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-
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262
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# Get the reference sequence name from a target index
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attach_function \
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:sam_hdr_tid2name,
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265
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[SamHdr, :int],
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:string
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-
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# Get the reference sequence length from a target index
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attach_function \
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270
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:sam_hdr_tid2len,
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[SamHdr, :int],
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272
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:hts_pos_t
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-
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# Generate a unique \@PG ID: value
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attach_function \
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:sam_hdr_pg_id,
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[SamHdr, :string],
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:string
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-
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# Add an \@PG line.
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attach_function \
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:sam_hdr_add_pg,
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[SamHdr, :string, :varargs],
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:int
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# A function to help with construction of CL tags in @PG records.
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attach_function \
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:stringify_argv,
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%i[int pointer],
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:string
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291
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-
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292
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# Increments the reference count on a header
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293
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attach_function \
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:sam_hdr_incr_ref,
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[SamHdr],
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:void
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297
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-
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298
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# Create a new bam1_t alignment structure
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299
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attach_function \
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300
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:bam_init1,
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[],
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Bam1.by_ref
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-
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# Destroy a bam1_t structure
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attach_function \
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:bam_destroy1,
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[Bam1],
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:void
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309
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-
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# Read a BAM format alignment record
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attach_function \
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:bam_read1,
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[BGZF, Bam1],
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:int
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-
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# Write a BAM format alignment record
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attach_function \
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:bam_write1,
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[BGZF, Bam1],
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:int
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321
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-
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322
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# Copy alignment record data
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323
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attach_function \
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:bam_copy1,
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[Bam1, Bam1],
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Bam1.by_ref
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-
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328
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# Create a duplicate alignment record
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329
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attach_function \
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330
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:bam_dup1,
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[Bam1],
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332
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Bam1.by_ref
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333
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-
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334
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# Calculate query length from CIGAR data
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335
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attach_function \
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336
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:bam_cigar2qlen,
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337
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%i[int pointer],
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:int64
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339
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-
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340
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# Calculate reference length from CIGAR data
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341
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attach_function \
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342
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:bam_cigar2rlen,
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343
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%i[int pointer],
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344
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:hts_pos_t
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345
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-
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346
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# Calculate the rightmost base position of an alignment on the reference genome.
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347
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attach_function \
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348
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:bam_endpos,
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349
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[Bam1],
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350
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:hts_pos_t
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351
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-
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352
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attach_function \
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353
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:bam_str2flag,
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354
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[:string],
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355
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:int
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356
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-
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357
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attach_function \
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358
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:bam_flag2str,
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359
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[:int],
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360
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:string
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361
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-
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362
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# Set the name of the query
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363
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attach_function \
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364
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:bam_set_qname,
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365
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[Bam1, :string],
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366
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:int
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367
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-
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368
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# Initialise fp->idx for the current format type for SAM, BAM and CRAM types .
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369
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attach_function \
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370
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:sam_idx_init,
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371
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[HtsFile, SamHdr, :int, :string],
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372
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:int
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373
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-
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374
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# Writes the index initialised with sam_idx_init to disk.
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375
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attach_function \
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376
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:sam_idx_save,
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377
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[HtsFile],
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378
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:int
|
379
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-
|
380
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# Load a BAM (.csi or .bai) or CRAM (.crai) index file
|
381
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attach_function \
|
382
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:sam_index_load,
|
383
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[HtsFile, :string],
|
384
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-
HtsIdx.by_ref
|
385
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-
|
386
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# Load a specific BAM (.csi or .bai) or CRAM (.crai) index file
|
387
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attach_function \
|
388
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-
:sam_index_load2,
|
389
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[HtsFile, :string, :string],
|
390
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-
HtsIdx.by_ref
|
391
|
-
|
392
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-
# Load or stream a BAM (.csi or .bai) or CRAM (.crai) index file
|
393
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-
attach_function \
|
394
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-
:sam_index_load3,
|
395
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[HtsFile, :string, :string, :int],
|
396
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-
HtsIdx.by_ref
|
397
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-
|
398
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# Generate and save an index file
|
399
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-
attach_function \
|
400
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-
:sam_index_build,
|
401
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-
%i[string int],
|
402
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-
:int
|
403
|
-
|
404
|
-
# Generate and save an index to a specific file
|
405
|
-
attach_function \
|
406
|
-
:sam_index_build2,
|
407
|
-
%i[string string int],
|
408
|
-
:int
|
409
|
-
|
410
|
-
# Generate and save an index to a specific file
|
411
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-
attach_function \
|
412
|
-
:sam_index_build3,
|
413
|
-
%i[string string int int],
|
414
|
-
:int
|
415
|
-
|
416
|
-
# Create a BAM/CRAM iterator
|
417
|
-
attach_function \
|
418
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-
:sam_itr_queryi,
|
419
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-
[HtsIdx, :int, :hts_pos_t, :hts_pos_t],
|
420
|
-
HtsItr.by_ref
|
421
|
-
|
422
|
-
# Create a SAM/BAM/CRAM iterator
|
423
|
-
attach_function \
|
424
|
-
:sam_itr_querys,
|
425
|
-
[HtsIdx, SamHdr, :string],
|
426
|
-
HtsItr.by_ref
|
427
|
-
|
428
|
-
# Create a multi-region iterator
|
429
|
-
attach_function \
|
430
|
-
:sam_itr_regions,
|
431
|
-
[HtsIdx, SamHdr, :pointer, :uint],
|
432
|
-
HtsItr.by_ref
|
433
|
-
|
434
|
-
# Create a multi-region iterator
|
435
|
-
attach_function \
|
436
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-
:sam_itr_regarray,
|
437
|
-
[HtsIdx, SamHdr, :pointer, :uint],
|
438
|
-
HtsItr.by_ref
|
439
|
-
|
440
|
-
# Get the next read from a SAM/BAM/CRAM iterator
|
441
|
-
def self.sam_itr_next(htsfp, itr, r)
|
442
|
-
# FIXME: check if htsfp is compressed BGZF
|
443
|
-
hts_log_error("Null iterator") if itr.null?
|
444
|
-
# FIXME: check multi
|
445
|
-
hts_itr_next(htsfp[:fp][:bgzf], itr, r, htsfp)
|
446
|
-
end
|
447
|
-
|
448
|
-
attach_function \
|
449
|
-
:sam_parse_region,
|
450
|
-
[SamHdr, :string, :pointer, :pointer, :pointer, :int],
|
451
|
-
:string
|
452
|
-
|
453
|
-
# SAM I/O
|
454
|
-
|
455
|
-
# macros (or alias)
|
456
|
-
# sam_open
|
457
|
-
# sam_open_format
|
458
|
-
# sam_close
|
459
|
-
|
460
|
-
attach_function \
|
461
|
-
:sam_open_mode,
|
462
|
-
%i[string string string],
|
463
|
-
:int
|
464
|
-
|
465
|
-
# A version of sam_open_mode that can handle ,key=value options.
|
466
|
-
attach_function \
|
467
|
-
:sam_open_mode_opts,
|
468
|
-
%i[string string string],
|
469
|
-
:string
|
470
|
-
|
471
|
-
attach_function \
|
472
|
-
:sam_hdr_change_HD,
|
473
|
-
[SamHdr, :string, :string],
|
474
|
-
:int
|
475
|
-
|
476
|
-
attach_function \
|
477
|
-
:sam_parse1,
|
478
|
-
[KString, SamHdr, Bam1],
|
479
|
-
:int
|
480
|
-
|
481
|
-
attach_function \
|
482
|
-
:sam_format1,
|
483
|
-
[SamHdr, Bam1, KString],
|
484
|
-
:int
|
485
|
-
|
486
|
-
# Read a record from a file
|
487
|
-
attach_function \
|
488
|
-
:sam_read1,
|
489
|
-
[HtsFile, SamHdr, Bam1],
|
490
|
-
:int
|
491
|
-
|
492
|
-
# Write a record to a file
|
493
|
-
attach_function \
|
494
|
-
:sam_write1,
|
495
|
-
[HtsFile, SamHdr, Bam1],
|
496
|
-
:int
|
497
|
-
|
498
|
-
# Return a pointer to an aux record
|
499
|
-
attach_function \
|
500
|
-
:bam_aux_get,
|
501
|
-
[Bam1, :string], # FIXME
|
502
|
-
:pointer
|
503
|
-
|
504
|
-
# Get an integer aux value
|
505
|
-
attach_function \
|
506
|
-
:bam_aux2i,
|
507
|
-
[:pointer],
|
508
|
-
:int64
|
509
|
-
|
510
|
-
# Get an integer aux value
|
511
|
-
attach_function \
|
512
|
-
:bam_aux2f,
|
513
|
-
[:pointer],
|
514
|
-
:double
|
515
|
-
|
516
|
-
# Get a character aux value
|
517
|
-
attach_function \
|
518
|
-
:bam_aux2A,
|
519
|
-
[:pointer],
|
520
|
-
:string
|
521
|
-
|
522
|
-
# Get a string aux value
|
523
|
-
attach_function \
|
524
|
-
:bam_aux2Z,
|
525
|
-
[:pointer],
|
526
|
-
:string
|
527
|
-
|
528
|
-
# Get the length of an array-type ('B') tag
|
529
|
-
attach_function \
|
530
|
-
:bam_auxB_len,
|
531
|
-
[:pointer],
|
532
|
-
:uint
|
533
|
-
|
534
|
-
# Get an integer value from an array-type tag
|
535
|
-
attach_function \
|
536
|
-
:bam_auxB2i,
|
537
|
-
%i[pointer uint],
|
538
|
-
:int64
|
539
|
-
|
540
|
-
# Get a floating-point value from an array-type tag
|
541
|
-
attach_function \
|
542
|
-
:bam_auxB2f,
|
543
|
-
%i[pointer uint],
|
544
|
-
:double
|
545
|
-
|
546
|
-
# Append tag data to a bam record
|
547
|
-
attach_function \
|
548
|
-
:bam_aux_append,
|
549
|
-
[Bam1, :string, :string, :int, :pointer],
|
550
|
-
:int
|
551
|
-
|
552
|
-
# Delete tag data from a bam record
|
553
|
-
attach_function \
|
554
|
-
:bam_aux_del,
|
555
|
-
[Bam1, :pointer],
|
556
|
-
:int
|
557
|
-
|
558
|
-
# Update or add a string-type tag
|
559
|
-
attach_function \
|
560
|
-
:bam_aux_update_str,
|
561
|
-
[Bam1, :string, :int, :string],
|
562
|
-
:int
|
563
|
-
|
564
|
-
# Update or add an integer tag
|
565
|
-
attach_function \
|
566
|
-
:bam_aux_update_int,
|
567
|
-
[Bam1, :string, :int64],
|
568
|
-
:int
|
569
|
-
|
570
|
-
# Update or add a floating-point tag
|
571
|
-
attach_function \
|
572
|
-
:bam_aux_update_float,
|
573
|
-
[Bam1, :string, :float],
|
574
|
-
:int
|
575
|
-
|
576
|
-
# Update or add an array tag
|
577
|
-
attach_function \
|
578
|
-
:bam_aux_update_array,
|
579
|
-
[Bam1, :string, :uint8, :uint32, :pointer],
|
580
|
-
:int
|
581
|
-
|
582
|
-
# sets an iterator over multiple
|
583
|
-
attach_function \
|
584
|
-
:bam_plp_init,
|
585
|
-
%i[bam_plp_auto_f pointer],
|
586
|
-
:bam_plp
|
587
|
-
|
588
|
-
attach_function \
|
589
|
-
:bam_plp_destroy,
|
590
|
-
[:bam_plp],
|
591
|
-
:void
|
592
|
-
|
593
|
-
attach_function \
|
594
|
-
:bam_plp_push,
|
595
|
-
[:bam_plp, Bam1],
|
596
|
-
:int
|
597
|
-
|
598
|
-
attach_function \
|
599
|
-
:bam_plp_next,
|
600
|
-
%i[bam_plp pointer pointer pointer],
|
601
|
-
BamPileup1.by_ref
|
602
|
-
|
603
|
-
attach_function \
|
604
|
-
:bam_plp_auto,
|
605
|
-
%i[bam_plp pointer pointer pointer],
|
606
|
-
BamPileup1.by_ref
|
607
|
-
|
608
|
-
attach_function \
|
609
|
-
:bam_plp64_next,
|
610
|
-
%i[bam_plp pointer pointer pointer],
|
611
|
-
BamPileup1.by_ref
|
612
|
-
|
613
|
-
attach_function \
|
614
|
-
:bam_plp64_auto,
|
615
|
-
%i[bam_plp pointer pointer pointer],
|
616
|
-
BamPileup1.by_ref
|
617
|
-
|
618
|
-
attach_function \
|
619
|
-
:bam_plp_set_maxcnt,
|
620
|
-
%i[bam_plp int],
|
621
|
-
:void
|
622
|
-
|
623
|
-
attach_function \
|
624
|
-
:bam_plp_reset,
|
625
|
-
[:bam_plp],
|
626
|
-
:void
|
627
|
-
|
628
|
-
# sets a callback to initialise any per-pileup1_t fields.
|
629
|
-
attach_function \
|
630
|
-
:bam_plp_insertion,
|
631
|
-
[BamPileup1, KString, :pointer],
|
632
|
-
:int
|
633
|
-
|
634
|
-
# sets a callback to initialise any per-pileup1_t fields.
|
635
|
-
# bam_plp_constructor
|
636
|
-
|
637
|
-
# bam_plp_destructor
|
638
|
-
|
639
|
-
# Get pileup padded insertion sequence
|
640
|
-
# bam_plp_insertion
|
641
|
-
|
642
|
-
attach_function \
|
643
|
-
:bam_mplp_init,
|
644
|
-
%i[int bam_plp_auto_f pointer],
|
645
|
-
:bam_mplp
|
646
|
-
|
647
|
-
attach_function \
|
648
|
-
:bam_mplp_init_overlaps,
|
649
|
-
[:bam_mplp],
|
650
|
-
:int
|
651
|
-
|
652
|
-
attach_function \
|
653
|
-
:bam_mplp_destroy,
|
654
|
-
[:bam_mplp],
|
655
|
-
:void
|
656
|
-
|
657
|
-
attach_function \
|
658
|
-
:bam_mplp_set_maxcnt,
|
659
|
-
%i[bam_mplp int],
|
660
|
-
:void
|
661
|
-
|
662
|
-
attach_function \
|
663
|
-
:bam_mplp_auto,
|
664
|
-
%i[bam_mplp pointer pointer pointer pointer], # BamPileup1T
|
665
|
-
:int
|
666
|
-
|
667
|
-
attach_function \
|
668
|
-
:bam_mplp64_auto,
|
669
|
-
%i[bam_mplp pointer pointer pointer pointer], # BamPileup1T
|
670
|
-
:int
|
671
|
-
|
672
|
-
attach_function \
|
673
|
-
:bam_mplp_reset,
|
674
|
-
[:bam_mplp],
|
675
|
-
:void
|
676
|
-
|
677
|
-
# bam_mplp_constructor
|
678
|
-
# bam_mplp_destructor
|
679
|
-
|
680
|
-
attach_function \
|
681
|
-
:sam_cap_mapq,
|
682
|
-
[Bam1, :string, :hts_pos_t, :int],
|
683
|
-
:int
|
684
|
-
|
685
|
-
attach_function \
|
686
|
-
:sam_prob_realn,
|
687
|
-
[Bam1, :string, :hts_pos_t, :int],
|
688
|
-
:int
|
689
101
|
end
|
690
102
|
end
|