bio-graphics 1.0
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- data/doc/classes/Bio.html +135 -0
- data/doc/classes/Bio/Graphics.html +247 -0
- data/doc/classes/Bio/Graphics/Panel.html +344 -0
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- data/doc/classes/Bio/Graphics/Panel/Track/Feature.html +292 -0
- data/doc/classes/Bio/Graphics/Panel/Track/Feature.src/M000011.html +65 -0
- data/doc/classes/Bio/Graphics/Panel/Track/Feature.src/M000014.html +65 -0
- data/doc/classes/Bio/Graphics/Panel/Track/Feature/PixelRange.html +155 -0
- data/doc/classes/Bio/Graphics/Panel/Track/Feature/PixelRange.src/M000012.html +18 -0
- data/doc/classes/Bio/Graphics/Panel/Track/Feature/PixelRange.src/M000015.html +18 -0
- data/doc/classes/ImageMap.html +185 -0
- data/doc/classes/ImageMap.src/M000001.html +18 -0
- data/doc/classes/ImageMap.src/M000002.html +24 -0
- data/doc/classes/ImageMap/ImageMapElement.html +187 -0
- data/doc/classes/ImageMap/ImageMapElement.src/M000003.html +19 -0
- data/doc/classes/ImageMap/ImageMapElement.src/M000004.html +20 -0
- data/doc/created.rid +1 -0
- data/doc/files/README_DEV.html +432 -0
- data/doc/files/TUTORIAL.html +358 -0
- data/doc/files/lib/bio-graphics_rb.html +121 -0
- data/doc/files/lib/bio/graphics/feature_rb.html +113 -0
- data/doc/files/lib/bio/graphics/image_map_rb.html +113 -0
- data/doc/files/lib/bio/graphics/panel_rb.html +113 -0
- data/doc/files/lib/bio/graphics/ruler_rb.html +113 -0
- data/doc/files/lib/bio/graphics/track_rb.html +113 -0
- data/doc/fr_class_index.html +35 -0
- data/doc/fr_file_index.html +34 -0
- data/doc/fr_method_index.html +41 -0
- data/doc/images/example.png +0 -0
- data/doc/images/glyph_showcase.png +0 -0
- data/doc/images/terms.png +0 -0
- data/doc/images/terms.svg +166 -0
- data/doc/index.html +24 -0
- data/images/example.png +0 -0
- data/images/glyph_showcase.png +0 -0
- data/images/terms.png +0 -0
- data/images/terms.svg +166 -0
- data/lib/bio-graphics.rb +18 -0
- data/lib/bio/graphics/feature.rb +136 -0
- data/lib/bio/graphics/image_map.rb +37 -0
- data/lib/bio/graphics/panel.rb +205 -0
- data/lib/bio/graphics/ruler.rb +96 -0
- data/lib/bio/graphics/track.rb +387 -0
- data/samples/arkdb_features.rb +37 -0
- data/samples/data.txt +32 -0
- data/samples/glyph_showcase.rb +29 -0
- metadata +137 -0
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<div id="fileHeader">
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<h1>TUTORIAL</h1>
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<table class="header-table">
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<tr class="top-aligned-row">
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<td><strong>Path:</strong></td>
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<td>TUTORIAL
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</td>
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</tr>
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<tr class="top-aligned-row">
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<td><strong>Last Update:</strong></td>
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<td>Sat Sep 29 18:23:07 +0100 2007</td>
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</tr>
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<!-- banner header -->
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<div id="contextContent">
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<div id="description">
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<p>
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<img src="../images/example.png" />
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</p>
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<h1><a href="../classes/Bio/Graphics.html">Bio::Graphics</a> tutorial</h1>
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<p>
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Copyright (C) 2007 Jan Aerts <jan.aerts@bbsrc.ac.uk>
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</p>
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<p>
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License: The Ruby License
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</p>
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<p>
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Homepage: <a
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href="http://bio-graphics.rubyforge.org">bio-graphics.rubyforge.org</a>
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</p>
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<p>
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(If you‘d like to know how the code itself works, please see the
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README.DEV)
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</p>
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<h2>Overview and terms used</h2>
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<p>
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This <a href="../classes/Bio/Graphics.html">Bio::Graphics</a> library
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allows for drawing overviews of genomic regions, similar to the pictures
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drawn by <a href="http://www.gmod.org/wiki/index.php/Gbrowse">gbrowse</a>.
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Basically, it allows creating simple images that display features on a
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linear map. The code is based on the equivalent code in the <a
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href="http://www.bioperl.org/wiki/Main_Page">bioperl</a> project.
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</p>
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<p>
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Any <a href="../classes/Bio/Graphics.html">Bio::Graphics</a> image consists
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of the following parts (nomenclature identical to that from bioperl):
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</p>
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<ul>
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<li>one panel: container of all tracks
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</li>
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<li>one or more tracks: container of the features. Multiple tracks can exist in
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the same graphic to allow for differential visualization of different
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feature types (e.g. genes as blue rectangles and polymorphisms as red
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triangles)
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</li>
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<li>one or more features in each track: these are the actual features that you
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want to display (e.g. ‘gene 1’, ‘SNP 123445’)
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</li>
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<li>a ruler on top of the panel: is added automatically
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</li>
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</ul>
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<p>
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Schematically:
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</p>
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<pre>
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panel
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+-> track 1
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| +-> feature 1
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| +-> feature 2
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| +-> feature 3
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+-> track 2
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| +-> feature 4
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| +-> feature 5
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+-> ruler
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</pre>
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<p>
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Below is an annotated version of the picture at the top of the page. The
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whole picture makes up the panel, which contains one ruler and 3 tracks
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(‘scaffolds’, ‘markers’ and ‘clones’).
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Each of these tracks has zero or more features.
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</p>
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<p>
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<img src="../images/terms.png" />
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</p>
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<p>
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As a feature can only exist within the confines of a track and a track can
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only exist within the confines of a panel, these classes are encapsuled.
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This means that the Track class is not Bio::Graphics::Track, but <a
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href="../classes/Bio/Graphics/Panel/Track.html">Bio::Graphics::Panel::Track</a>;
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the same goes for <a
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href="../classes/Bio/Graphics/Panel/Track/Feature.html">Bio::Graphics::Panel::Track::Feature</a>.
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</p>
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<h2>Glyphs</h2>
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<p>
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According to Wikipedia, a <em>glyph</em> is "the shape given in a
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particular typeface to a specific grapheme or symbol". In the context
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of <a href="../classes/Bio/Graphics.html">Bio::Graphics</a>, a glyph is
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just the thing that represents one single feature. Several kinds of glyph
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exist to allow for features that are on a particular strand as well as for
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spliced features. See the picture below for an overview.
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</p>
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<p>
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<img src="../images/glyph_showcase.png" />
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</p>
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<p>
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If the view is really zoomed out, arrow on the directed glyphs are not
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shown, as they would be bigger than the glyph itself.
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</p>
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<h2>Use</h2>
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<p>
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The code below was used to create the picture showing all the different
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glyphs mentioned above. This is not the nicest piece of code, but it is the
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simplest. It is straightforward to parse a GFF file and create a picture
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based on that. See my <a
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href="http://saaientist.blogspot.com/2007/09/graphics-genomics-and-ruby.html">blog</a>
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for an nicer example.
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</p>
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<p>
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For an explanation of the arguments with each method for this example, see
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the <a href="../classes/Bio/Graphics/Panel.html">Bio::Graphics::Panel</a>
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and <a
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href="../classes/Bio/Graphics/Panel/Track.html">Bio::Graphics::Panel::Track</a>
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object documentation.
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</p>
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<pre>
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1 # Load the library
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2 require 'bio-graphics' # or require_gem 'bio-graphics'
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3
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4 # Create the panel
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5 my_panel = Bio::Graphics::Panel.new(500, 600, true, 1, 500)
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6
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7 # Create the tracks
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8 generic_track = my_panel.add_track('generic')
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9 directed_track = my_panel.add_track('directed',[0,1,0],'directed_generic')
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10 triangle_track = my_panel.add_track('triangle',[1,0,0],'triangle')
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11 spliced_track = my_panel.add_track('spliced',[1,0,0],'spliced')
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12 directed_spliced_track = my_panel.add_track('directed_spliced',[1,0,1],'directed_spliced')
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13
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14 # Add features to each track
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15 generic_track.add_feature('clone1','250..375', 'http://www.newsforge.com')
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16 generic_track.add_feature('clone2','54..124', 'http://www.thearkdb.org')
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17 generic_track.add_feature('clone3','100..449', 'http://www.google.com')
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18
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19 directed_track.add_feature('marker1','50..60', 'http://www.google.com')
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20 directed_track.add_feature('marker2','complement(80..120)', 'http://www.sourceforge.net')
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21
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22 triangle_track.add_feature('snp1','56')
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23 triangle_track.add_feature('snp2','103','http://digg.com')
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24
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25 spliced_track.add_feature('gene1','join(34..52,109..183)','http://news.bbc.co.uk')
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26 spliced_track.add_feature('gene2','complement(join(170..231,264..299,350..360,409..445))')
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27 spliced_track.add_feature('gene3','join(134..152,209..283)')
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28
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29 directed_spliced_track.add_feature('gene4','join(34..52,109..183)', 'http://www.vrtnieuws.net')
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30 directed_spliced_track.add_feature('gene5','complement(join(170..231,264..299,350..360,409..445))')
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31 directed_spliced_track.add_feature('gene6','join(134..152,209..283)')
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32
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33 # Draw the thing
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34 my_panel.draw('glyph_showcase.png')
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</pre>
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<p>
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Let‘s walk through this:
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</p>
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<ul>
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<li>Line 2: load the actual library code. Make sure that the library is in your
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RUBYLIB path.
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</li>
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<li>Line 5: Create the panel, which is nothing more than the canvas
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you‘ll be working on. The following parameters were used:
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<ul>
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<li>The sequence is 500 bp long.
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</li>
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<li>The width of the picture will be 600 points.
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</li>
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<li>A HTML page will be created to make hyperlinks possible (the <em>true</em>)
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</li>
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<li>The picture will be zoomed in from bp 1 to 500 (i.e. completely zoomed
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out).
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</li>
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</ul>
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</li>
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<li>Lines 8-12: Create the different tracks. Parameters are:
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<ul>
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<li>name.
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</li>
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<li>colour. At the moment in RGB. (This will probably change to colour names in
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the future).
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</li>
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<li>type. See the picture above and Bio::Graphics::Panel::Track#new for allowed
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values.
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</li>
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</ul>
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</li>
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<li>Lines 15-31: Add features to each track. Parameters are:
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<ul>
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<li>name.
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</li>
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<li>location. This should be a location parsable by <a
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href="http://www.bioruby.org">bioruby's</a> Bio::Locations object.
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</li>
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<li>link.
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</li>
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</ul>
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</li>
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<li>Line 34: Create the actual picture. If the third parameter to the initial
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Panel#new call (in line 5) is ‘true’, a HTML file is created as
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well.
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</li>
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</ul>
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<h3>Hyperlinks</h3>
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<p>
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There are no graphic formats that are inherently clickable (SVG is a
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special case and the <em>display</em> of it is not the SVG itself).
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Therefore, in case the user wants a clickable graphic, an accompanying HTML
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file is created with the same name as the picture but obviously the .html
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extension. This file contains the map and will display a clickable picture
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when loaded in a webbrowser.
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</p>
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<h2>Download and installation</h2>
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<p>
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Development of the library is at <a
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href="http://rubyforge.org/projects/bio-graphics">rubyforge.org/projects/bio-graphics</a>/
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<td><strong>Last Update:</strong></td>
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<h1>bio-graphics.rb - Loading all <a href="../../classes/Bio/Graphics.html">Bio::Graphics</a> modules</h1>
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<table>
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<tr><td valign="top">Copyright:</td><td>Copyright (C) 2007 Jan Aerts <jan.aerts@bbsrc.ac.uk>
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<tr><td valign="top">License:</td><td>The Ruby License
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bio
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cairo
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