bio-assembly 0.0.2 → 0.1.0

This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
Files changed (16) hide show
  1. data/Gemfile +2 -0
  2. data/LICENSE.txt +1 -1
  3. data/README.rdoc +43 -3
  4. data/Rakefile +1 -1
  5. data/VERSION +1 -1
  6. data/bio-assembly.gemspec +13 -5
  7. data/data/example.caf +2755 -0
  8. data/lib/bio-assembly.rb +4 -14
  9. data/lib/bio-assembly/ace.rb +11 -19
  10. data/lib/bio-assembly/caf.rb +124 -0
  11. data/lib/bio-assembly/contig.rb +5 -1
  12. data/lib/bio-assembly/maf.rb +15 -0
  13. data/lib/bio-assembly/read.rb +4 -0
  14. data/test/{test_bio-assembly.rb → test_bio-assembly-ace.rb} +15 -7
  15. data/test/test_bio-assembly-caf.rb +54 -0
  16. metadata +47 -25
data/Gemfile CHANGED
@@ -3,6 +3,8 @@ source "http://rubygems.org"
3
3
  # Example:
4
4
  # gem "activesupport", ">= 2.3.5"
5
5
 
6
+ gem "bio", ">= 1.4.1"
7
+
6
8
  # Add dependencies to develop your gem here.
7
9
  # Include everything needed to run rake, tests, features, etc.
8
10
  group :development do
data/LICENSE.txt CHANGED
@@ -1,4 +1,4 @@
1
- Copyright (c) 2010 Chase Miller
1
+ Copyright (c) 2010 Chase Miller and Francesco Strozzi
2
2
 
3
3
  Permission is hereby granted, free of charge, to any person obtaining
4
4
  a copy of this software and associated documentation files (the
data/README.rdoc CHANGED
@@ -2,14 +2,54 @@
2
2
 
3
3
  BioRuby plugin for parsing, writing, and maniuplating assembly data
4
4
 
5
+ Currently supports ACE and CAF formats
6
+
5
7
  == Install
6
8
 
7
9
  gem install bio-assembly
8
10
 
9
11
  == Usage
10
12
 
11
- Examples on my blog:
12
- http://chasemiller4.blogspot.com/2010/10/bioruby-ace-parser-example.html
13
+ require 'bio-assembly'
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+
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+ # ace file path
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+ asm = Bio::Assembly.new("data.ace", :ace)
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+
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+ # iterate through contigs (streams each contig)
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+ asm.each_contig do |contig|
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+
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+ # print name and consensus seq
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+ puts contig.name
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+ puts contig.consensus_seq
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+
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+ # iterate through reads
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+ contig.each_read do |read|
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+
28
+ # print name, sequence and orientation
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+ puts read.name
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+ puts read.seq
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+ puts read.orientation
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+
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+ # print the postion of the read
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+ # in relation to the consensus seq
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+ puts read.from
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+ puts read.to
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+
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+ # print the portion of the read
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+ # that was used to create the consensus
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+ puts read.clear_range_from
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+ puts read.clear_range_to
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+
43
+ end
44
+
45
+ # grab the reads that make up a particular region of the contig
46
+ reads_in_region = contig.find_reads_in_range(10, 50)
47
+
48
+ end
49
+
50
+ # experimental - output ace
51
+ file = File.new('out.ace', 'w')
52
+ file.puts asm.to_ace
13
53
 
14
54
  == Contributing to bio-assembly
15
55
 
@@ -23,6 +63,6 @@ http://chasemiller4.blogspot.com/2010/10/bioruby-ace-parser-example.html
23
63
 
24
64
  == Copyright
25
65
 
26
- Copyright (c) 2010 Chase Miller. See LICENSE.txt for
66
+ Copyright (c) 2011 Chase Miller and Francesco Strozzi. See LICENSE.txt for
27
67
  further details.
28
68
 
data/Rakefile CHANGED
@@ -18,7 +18,7 @@ Jeweler::Tasks.new do |gem|
18
18
  gem.summary = "BioRuby Assembly plugin"
19
19
  gem.description = "bioruby plugin to parse, write, and manipulate assembly data"
20
20
  gem.email = "chmille4@gmail.com"
21
- gem.authors = ["Chase Miller"]
21
+ gem.authors = ["Chase Miller","Francesco Strozzi"]
22
22
  # Include your dependencies below. Runtime dependencies are required when using your gem,
23
23
  # and development dependencies are only needed for development (ie running rake tasks, tests, etc)
24
24
  # gem.add_runtime_dependency 'jabber4r', '> 0.1'
data/VERSION CHANGED
@@ -1 +1 @@
1
- 0.0.2
1
+ 0.1.0
data/bio-assembly.gemspec CHANGED
@@ -5,11 +5,11 @@
5
5
 
6
6
  Gem::Specification.new do |s|
7
7
  s.name = %q{bio-assembly}
8
- s.version = "0.0.2"
8
+ s.version = "0.1.0"
9
9
 
10
10
  s.required_rubygems_version = Gem::Requirement.new(">= 0") if s.respond_to? :required_rubygems_version=
11
- s.authors = ["Chase Miller"]
12
- s.date = %q{2010-12-27}
11
+ s.authors = ["Chase Miller", "Francesco Strozzi"]
12
+ s.date = %q{2011-01-12}
13
13
  s.description = %q{bioruby plugin to parse, write, and manipulate assembly data}
14
14
  s.email = %q{chmille4@gmail.com}
15
15
  s.extra_rdoc_files = [
@@ -25,13 +25,17 @@ Gem::Specification.new do |s|
25
25
  "Rakefile",
26
26
  "VERSION",
27
27
  "bio-assembly.gemspec",
28
+ "data/example.caf",
28
29
  "data/example1.ace",
29
30
  "lib/bio-assembly.rb",
30
31
  "lib/bio-assembly/ace.rb",
32
+ "lib/bio-assembly/caf.rb",
31
33
  "lib/bio-assembly/contig.rb",
34
+ "lib/bio-assembly/maf.rb",
32
35
  "lib/bio-assembly/read.rb",
33
36
  "test/helper.rb",
34
- "test/test_bio-assembly.rb"
37
+ "test/test_bio-assembly-ace.rb",
38
+ "test/test_bio-assembly-caf.rb"
35
39
  ]
36
40
  s.homepage = %q{http://github.com/chmille4/bioruby-assembly}
37
41
  s.licenses = ["MIT"]
@@ -40,7 +44,8 @@ Gem::Specification.new do |s|
40
44
  s.summary = %q{BioRuby Assembly plugin}
41
45
  s.test_files = [
42
46
  "test/helper.rb",
43
- "test/test_bio-assembly.rb"
47
+ "test/test_bio-assembly-ace.rb",
48
+ "test/test_bio-assembly-caf.rb"
44
49
  ]
45
50
 
46
51
  if s.respond_to? :specification_version then
@@ -48,12 +53,14 @@ Gem::Specification.new do |s|
48
53
  s.specification_version = 3
49
54
 
50
55
  if Gem::Version.new(Gem::VERSION) >= Gem::Version.new('1.2.0') then
56
+ s.add_runtime_dependency(%q<bio>, [">= 1.4.1"])
51
57
  s.add_development_dependency(%q<shoulda>, [">= 0"])
52
58
  s.add_development_dependency(%q<bundler>, ["~> 1.0.0"])
53
59
  s.add_development_dependency(%q<jeweler>, ["~> 1.5.2"])
54
60
  s.add_development_dependency(%q<rcov>, [">= 0"])
55
61
  s.add_development_dependency(%q<bio>, [">= 1.4.1"])
56
62
  else
63
+ s.add_dependency(%q<bio>, [">= 1.4.1"])
57
64
  s.add_dependency(%q<shoulda>, [">= 0"])
58
65
  s.add_dependency(%q<bundler>, ["~> 1.0.0"])
59
66
  s.add_dependency(%q<jeweler>, ["~> 1.5.2"])
@@ -61,6 +68,7 @@ Gem::Specification.new do |s|
61
68
  s.add_dependency(%q<bio>, [">= 1.4.1"])
62
69
  end
63
70
  else
71
+ s.add_dependency(%q<bio>, [">= 1.4.1"])
64
72
  s.add_dependency(%q<shoulda>, [">= 0"])
65
73
  s.add_dependency(%q<bundler>, ["~> 1.0.0"])
66
74
  s.add_dependency(%q<jeweler>, ["~> 1.5.2"])
data/data/example.caf ADDED
@@ -0,0 +1,2755 @@
1
+ DNA : 22ak93c2.r1t
2
+ GTCGCnCATAAGATTACGAGATCTCGAGCTCGGTACCCTTCAAGCGATTCTCCTGCCTCA
3
+ GCCTCCCGAGTAGCTGGGATTATAGACTGTGCGTGCGCCACCATGCCTGGCTAATTTTTG
4
+ TATTTTTAGTAGGGATGGGGTTTCACCATGTTGGCCAAGCTGGTCTCGAGCTCCTGACCT
5
+ AGGATTACAGGCCTAAGCCACCGCACCCGGCATGATGGGTCTTTATTCTTCAAAGCAGGA
6
+ GGAAGGGATCCTAgAAAAACAGAGACAAGGCCAAACATGGTAgCTCACACCTGTAATCCC
7
+ AnCACTTTGGGAGGCCagTGCGGGTGAATCACGAAGTCAGGAGTTCAAGACCACCCTGGC
8
+ CAACATGGTGTAACACCTGCTCTCCTAAAATTAAACAAAATTTCATGGTTTGCGTGGGCC
9
+ GTCTTGTCTCATCACTTCACTCCTGAGGGCCGGCGCCGGAAAGATATCTTGATCTGCGGC
10
+ GCTCCGACCGTTTTCTTTAAACCTTACAACTCCCGACCTCCTCGCCTATCCTCCCTAAAT
11
+ CCTCGCCAGGCTCGCCTGCTTCAGCCACTCTTTCCTTCGCACCCTCCCCTCTCTTCAATA
12
+ TACTTCACCCGCCCATCCTTCACGCCGGCACGTATCCAATCTCTTCTTATCTTTCCGTAT
13
+ CCAAnTCCCTTCTCCCTCTGCCGCGACCTTCGCCATCCCTCTGCGCGTCCTCTTCC
14
+
15
+ BaseQuality : 22ak93c2.r1t
16
+ 4 4 8 4 4 4 4 4 4 4 4 4 6 8 17 21 14 7 6 6 6 7 7 6 8 14 16 21 15 20 20
17
+ 24 26 21 18 18 14 14 19 23 10 8 8 15 20 16 29 26 34 29 39 29 31 29 31
18
+ 34 32 27 27 25 19 19 24 31 33 36 34 34 34 26 27 22 32 32 36 28 28 15
19
+ 15 15 28 28 34 30 12 12 22 27 31 31 31 31 31 23 24 27 21 24 24 29 27
20
+ 27 27 34 34 36 38 38 38 36 36 40 36 37 38 45 45 36 34 33 31 31 34 34
21
+ 33 33 28 28 27 23 24 11 11 10 10 18 25 21 20 17 17 17 20 15 24 18 24
22
+ 26 23 23 18 20 25 23 30 30 30 33 33 37 37 32 37 37 32 45 35 37 37 37
23
+ 40 36 49 49 36 36 34 33 20 15 9 9 8 7 12 22 21 28 28 30 33 36 36 36 34
24
+ 31 31 25 31 28 26 26 24 20 17 9 11 8 9 10 23 23 31 23 23 15 9 9 15 33
25
+ 26 33 33 31 25 25 22 31 24 23 12 10 12 11 9 8 9 7 7 8 8 9 18 12 9 9 18
26
+ 20 26 31 21 21 9 8 8 11 13 21 21 23 15 15 15 15 15 17 17 9 7 9 19 20
27
+ 21 21 25 25 25 25 25 23 23 9 9 9 21 16 24 24 24 24 26 33 33 33 31 31
28
+ 27 15 17 7 4 4 4 16 20 27 33 34 34 23 15 14 8 9 6 6 9 1 14 16 8 11 15
29
+ 23 25 34 36 31 33 16 16 6 6 6 9 8 14 9 11 11 9 13 13 10 8 10 9 9 7 8
30
+ 20 20 20 14 14 10 10 10 16 8 8 6 6 8 9 10 7 8 8 8 8 6 8 6 6 8 12 9 8 7
31
+ 13 10 8 8 9 8 8 8 9 6 6 6 6 8 6 6 6 6 6 10 11 10 12 12 10 7 6 6 7 6 6
32
+ 7 8 7 6 6 7 6 8 6 6 8 8 8 8 6 6 6 8 6 6 8 6 6 11 15 9 9 9 9 9 9 9 9 9
33
+ 9 8 9 9 6 6 6 7 9 6 6 6 7 7 7 8 9 7 7 9 9 11 8 11 11 11 9 10 9 8 8 8
34
+ 10 6 6 9 8 6 6 6 6 8 8 9 11 9 8 8 8 8 8 8 8 9 9 9 10 10 8 6 6 8 6 6 10
35
+ 11 10 6 6 8 6 6 6 6 8 11 8 9 8 8 8 8 8 9 6 6 9 8 8 8 6 6 13 15 12 12 8
36
+ 8 6 6 8 9 8 8 9 8 8 6 6 6 8 8 6 6 6 8 8 8 6 6 8 6 6 8 6 6 9 8 8 6 6 8
37
+ 8 6 6 8 6 7 8 9 10 11 11 10 9 9 8 10 9 10 10 8 9 13 8 8 8 13 13 11 7 7
38
+ 7 11 8 8 10 11 10 9 9 14 7 7 10 10 10 8 8 8 8 6 7 8 8 6 6 6 6 6 6 6 6
39
+ 6 8 9 10 8 8 6 6 8 8 8 8 8 8 8 8 8 8 6 6 8 8 9 10 10 4 4 4 6 7 6 6 6 6
40
+ 10 8 8 9 15 11 6 6 8 8 8 8 8 8 9 9 8 9 7 7 8 6 6 8 13 10 8 8 7 6 8 6 6
41
+ 8 6 7 8 8 8 8 8 8 8 4
42
+
43
+
44
+ Sequence : 22ak93c2.r1t
45
+ Is_read
46
+ Padded
47
+ Staden_id 11
48
+ Clipping QUAL 39 331
49
+ Align_to_SCF 1 43 1 43
50
+ Align_to_SCF 44 317 45 318
51
+ Align_to_SCF 319 716 319 716
52
+ SCF_File 22ak93c2.r1tSCF
53
+ Primer Universal_primer
54
+ Strand Reverse
55
+ Dye Dye_terminator
56
+ Template 22ak93c2
57
+ Clone bK216E10
58
+ Sequencing_vector "m13mp18"
59
+ Seq_vec SVEC 1 38 "M13mp18"
60
+ Tag ALUS 43 180
61
+ Tag DONE 43 43 "AUTO-EDIT: deleted C at 43 (terminator, isolated, strong)"
62
+ Tag DONE 254 254 "AUTO-EDIT: replaced T by g at 254 (terminator, isolated, strong)"
63
+ Tag ALUS 269 402
64
+ Tag DONE 283 283 "AUTO-EDIT: replaced T by g at 283 (terminated, isolated, strong)"
65
+ Tag AMBG 298 302 "AUTOEDIT: Check this edit cluster!"
66
+ Tag DONE 317 317 "AUTO-EDIT: replaced C by a at 317 (terminated, compound, strong)"
67
+ Tag DONE 318 318 "AUTO-EDIT: inserted g at 318 (terminated, compound, strong)"
68
+
69
+ DNA : 22ak93c1.r1ta
70
+ GGGGGGATGATACAATTCGAGCTCGGTACCTTCAAGCGATTCTCCTGCCTCAGCCTCCCG
71
+ AGTAGCTGGGATTATAAACTGTGCGTGCGCCACCATGCCTGGCTAATTTTTGTATTTTTA
72
+ GTAGGGATGGGGTTTCACCATGTTGGCCAaGCTGGTCTCGAGCTCCTGACCTAGGATtAC
73
+ AGGCCTAaGCCACCGCACCCGGCATGATGGGTCTTTATTCTTCAAAGCAgGAGGAAGGGA
74
+ TCCTAGAAAAACAGAGACAAgGCCAAACATGGTAGCTCACACCTGTAATCCCAGCACTTT
75
+ GGGAgGCCAgTGCGGGTGAATCACgAAGTCAGGAGTTCAAGACCATCCTGGCCAACATGG
76
+ TGTAACACCGTCTCTCCTAGGATAAACCGAAATCACCTGGGTGTCCTGGCAAGTGCCTGT
77
+ CATCCCTGACCACTTGGAAAGCTGAGCCGAATCCTTATCCCnGAAAGTAAGGTTACGTGA
78
+ ACCCACATCAAACCACTGCGCTCCAGCCCAAAACATCGTGTTATACTCTGTCGCGACATT
79
+ GATGCCCAAAAAACAGGACCGCTATTGGGAGACACTCCCGGGTAATTGGGATTGAGGAGG
80
+ TTAAAGAAAACTCTTGATCTGAGAAAAAAGACCCTATCCTGGCGAGAAAACCACATGTGG
81
+ ACCACACCGCTTCACTTTGTCTAAGCAAAAAACGCCCCACTTGAAGATGACCCCCCCAGC
82
+ TGAAATCTTCCATTAATCCCCGAACATAAAGGTTTAACCCAAAATTTCCTGTTAGACCGC
83
+ CTTCTGAAGTTTCTAAAATTCTATGGGTAGGGACAAAAGTCCCTCGTTGAATTTCTAGGA
84
+ TACTTCCAAATATCCCCCACCTCTCCCCTGGGATCCCGATAGCTTTCTTGTCCCTAAAAA
85
+ AAACTTTGTTCAAACGGAGAAACCACACCCGGGGAAAATTCCCCTTTTCTTGGGGTTTAG
86
+ GTTTCTTTAAATAAATGCTGGGCACCAATCCTCAAATGAGTTCTTACCCAATCCCATATG
87
+ TTTTTAATCCTCAACAACAACCTTATTTCCTTAAGAGACCTCTCCTCGGAAATCATTTCA
88
+ AAACAAAAAAAACACTTAAGCCTTGGGTCTTTGGGACCCCCCAAAAAAAAAACATAAAGG
89
+ GGTGGCACCGTATACGCCTTGCCCATCTTTCTAGTGGAAGATAACGAAACGGTAGCTGGG
90
+ GCCAACCAGGCATCAGTGGGGGATACCGCTAAATGTTGGGGAACACCCAAAAACCCATCT
91
+ GCAGGCACCGCGAATGCGGAAAAGCGCACGCTATCACAAATGGTATATGGGCACC
92
+
93
+ BaseQuality : 22ak93c1.r1ta
94
+ 8 10 8 9 16 9 9 9 11 8 8 9 10 11 11 10 19 24 26 24 26 22 21 17 13 16
95
+ 11 11 10 10 14 14 19 22 24 26 32 32 33 29 29 29 27 27 27 27 27 32 32
96
+ 25 27 20 25 19 32 27 29 32 32 32 34 34 21 23 12 14 14 27 32 32 29 28
97
+ 27 34 31 34 34 34 37 34 32 32 30 30 28 28 28 30 30 33 33 33 30 30 34
98
+ 34 34 37 40 38 37 34 32 34 32 37 32 37 37 28 28 28 33 33 33 33 32 30
99
+ 30 29 28 21 23 14 12 30 32 45 45 41 41 30 25 22 33 33 34 23 21 18 12
100
+ 12 11 13 27 27 23 23 11 11 9 10 11 18 18 30 15 20 12 11 10 20 12 14 14
101
+ 32 32 33 28 28 18 23 18 12 12 20 12 16 13 18 14 15 12 32 23 21 10 10 9
102
+ 9 10 16 18 21 9 9 9 18 21 14 14 12 10 14 14 23 27 24 19 19 24 28 30 29
103
+ 29 34 34 29 25 17 12 12 16 14 14 23 10 10 10 11 15 21 24 18 25 11 11
104
+ 15 32 28 34 34 29 32 32 37 37 34 34 31 28 28 25 22 17 14 11 10 10 8 8
105
+ 9 14 8 8 12 17 18 15 13 18 17 17 10 10 10 22 16 24 30 24 28 27 32 19
106
+ 12 12 12 30 21 17 9 9 9 9 17 16 24 24 23 20 12 12 7 7 7 14 13 10 10 10
107
+ 23 23 22 15 17 18 24 24 15 17 11 10 8 8 8 13 20 25 17 17 17 20 20 18
108
+ 10 11 12 12 11 25 10 12 12 9 8 8 8 8 8 12 14 9 9 9 9 10 10 8 8 11 9 9
109
+ 9 14 20 20 19 15 15 15 15 11 11 6 6 9 8 10 12 12 15 15 10 9 10 11 7 7
110
+ 9 11 14 11 11 10 11 9 14 14 23 19 18 14 12 10 10 10 10 10 16 11 11 9 8
111
+ 8 9 8 11 11 9 8 8 10 9 12 13 9 9 8 8 8 8 8 6 6 11 6 6 8 11 9 9 9 6 6
112
+ 12 9 9 6 6 8 11 9 8 6 7 9 9 7 8 4 4 4 6 8 6 6 8 8 6 6 8 6 8 12 12 14
113
+ 10 9 10 11 9 8 7 7 7 7 10 8 8 6 6 8 6 6 8 10 8 6 6 10 8 10 9 10 10 9 8
114
+ 8 6 6 6 6 8 8 8 9 9 9 9 7 7 9 9 10 10 10 13 12 9 9 9 9 7 8 8 8 9 8 8 9
115
+ 9 9 8 6 6 8 6 6 6 7 7 9 9 10 8 8 7 7 7 9 10 10 8 6 6 6 6 6 10 10 8 6 6
116
+ 8 8 8 7 9 7 10 7 11 8 8 8 8 9 9 9 8 8 6 6 10 11 16 8 7 7 7 6 8 6 6 8
117
+ 10 11 9 9 9 7 7 8 7 7 7 7 7 8 11 9 16 16 24 22 15 9 8 13 13 12 12 9 9
118
+ 9 9 9 7 7 7 7 7 7 7 8 7 7 7 6 9 11 9 8 8 9 9 9 7 9 10 14 12 9 9 8 8 6
119
+ 6 6 6 8 6 6 8 6 6 6 6 6 9 8 10 10 9 10 10 13 10 11 6 7 11 6 6 8 6 6 6
120
+ 6 6 6 9 9 7 7 8 8 8 8 8 9 8 9 13 11 8 6 6 6 9 9 8 8 8 8 8 8 8 11 8 7
121
+ 10 12 7 7 9 8 6 6 8 8 7 7 10 8 8 7 7 7 8 8 8 8 7 7 7 7 7 10 9 10 12 10
122
+ 7 7 6 9 6 6 6 6 7 9 7 7 4 10 9 7 6 8 6 6 8 6 6 9 6 7 7 10 10 10 10 13
123
+ 13 8 9 8 8 8 6 7 10 11 10 9 8 6 6 6 6 6 8 8 7 9 7 7 7 7 7 7 7 10 10 10
124
+ 8 8 8 7 7 8 8 8 8 6 6 7 7 7 9 8 6 8 6 6 6 8 8 8 7 9 9 12 15 9 8 7 9 8
125
+ 8 9 8 8 6 8 12 11 7 7 7 7 7 7 9 11 11 9 9 9 10 9 9 9 8 8 8 8 8 7 7 12
126
+ 13 13 15 11 11 7 7 7 9 11 10 12 6 6 6 6 8 7 7 7 8 10 10 10 10 13 9 12
127
+ 11 11 6 6 6 6 8 8 6 7 7 12 10 13 9 8 7 7 10 7 7 15 17 15 12 17 20 15
128
+ 13 15 6 6 6 6 8 8 6 6 6 7 7 7 10 7 7 10 11 8 8 5 5 8 8 12 12 8 10 6 6
129
+ 6 6 8 8 7 7 7 12 10 7 7 9 7 7 9 9 8 9 10 10 8 8 9 11 9 8 9 9 11 11 9 9
130
+ 8 6 8 6 6 6 6 6 6 6 6 8 8 8 8 12 6 6 6 6 8 6 7 7 6 8 6 6 8 8 10 11 10
131
+ 12 7 8 8 9 12 6 6 6 6 6 6 6 6 8 9 9 9 9 9 8 7 8 7 7 7 8 7 7 7 7 9 9 9
132
+ 9 6 6 6 6 14 8 8 8 8 8 6 6 7 7 7 11 9 7 6 6 6 6 10 6 6 6 8 8 7 8 7 7 6
133
+ 7 7 8 8 12 13 15 15 21 9 10 9 8 8 10 6 6 6 6 4 4 4 8 8 8 7 6 6 6 6 6 6
134
+ 6 6 6 6 6 9 9 9 6 6 6 10 6 10 15 20 12 9 8 6 6 6 6 6 8 8 6 6 6 6 8 12
135
+ 13 12 10 9 8 11 8 10 9 7 7 4 4 6 6 8 4 4 4 6 8 6 6 6 8 8 13 10 8 9 9 9
136
+ 7 7 9 10 8 8 6 6 6 6 6 6 10 11 10 10 10 7 7 8 6 6 8 6 6 8 10 13 8 11 8
137
+ 11 10 6 6 6 7 11 14 11 11 6 6 8 8 8 9 9 9 9 7 7 10 10 6 6 6 6 6 6 8 8
138
+ 8 7 7 7 8 8 11 11 11 7 6 8 6 6 8 8 6 6 10 10 13 8 8 6 6 6 8 9 9 6 6 9
139
+ 9 8 8 6 6 8 6 6 8 8 4
140
+
141
+
142
+ Sequence : 22ak93c1.r1ta
143
+ Is_read
144
+ Padded
145
+ Staden_id 12
146
+ Clipping QUAL 31 335
147
+ Align_to_SCF 1 1315 1 1315
148
+ SCF_File 22ak93c1.r1taSCF
149
+ Primer Universal_primer
150
+ Strand Reverse
151
+ Dye Dye_terminator
152
+ Template 22ak93c1
153
+ Clone bK216E10
154
+ Sequencing_vector "m13mp18"
155
+ Seq_vec SVEC 1 30 "M13mp18"
156
+ Tag ALUS 31 172
157
+ Tag DONE 150 150 "AUTO-EDIT: replaced T by a at 150 (terminator, isolated, strong)"
158
+ Tag DONE 178 178 "AUTO-EDIT: replaced G by t at 178 (terminator, isolated, strong)"
159
+ Tag DONE 188 188 "AUTO-EDIT: replaced T by a at 188 (terminator, isolated, strong)"
160
+ Tag DONE 230 230 "AUTO-EDIT: replaced T by g at 230 (terminator, isolated, strong)"
161
+ Tag DONE 261 261 "AUTO-EDIT: replaced C by g at 261 (terminated, isolated, strong)"
162
+ Tag ALUS 262 446
163
+ Tag DONE 305 305 "AUTO-EDIT: replaced T by g at 305 (terminated, isolated, strong)"
164
+ Tag DONE 310 310 "AUTO-EDIT: inserted g at 310 (terminated, compound, strong)"
165
+ Tag DONE 325 325 "AUTO-EDIT: replaced A by g at 325 (terminated, isolated, strong)"
166
+
167
+ DNA : 22am91h5.r1t
168
+ GGGnnATAGATACGATCCGAGCTCGGTACCCATGTTGGCCAAGCTGGTCTCGAGCTCCTG
169
+ ACCTAGGATTACAGGCCTAAGCCACCGCACCCGGCATGATGGGTCTTTATTCTTCAAAGC
170
+ AGGAGGAAGGGATCCTAGAAAAACAGAGACAAGGCCAAACATGGTAGCTCACACCTGTAA
171
+ TCCCAGCACTTTGGGAGGCCAGTGCGGGTGAATCACGAAGTCAGGAGTTCAAGACCAGCC
172
+ TGGCCAACATGGTGAAACACCGTCTCTACTAAAATAAAAAGAAATTAGCTGGGTGTCGTG
173
+ GCAGGTGCCTGTAATCCCAGCCACTTGGGAAGCTGAGGCAGGAGAATCGCTTGAACCCGG
174
+ GAGGTGGAGGTTGCAnTGAGCCGAGATCACGCGACTGCACTCCAGCCCAACCAATAATGT
175
+ GAGACTCTGTCTCGAnAAnnnAAAGCnGAAAACAGACnnCTAGTACnGTACTTAACGGGG
176
+ TAATTATAATAATTAAATGAAAAAAATAnCTGTGAGGTGATTGATATAnTGCTGTTGCTA
177
+ AATACAACTAACATTTTAATATACCGGTGGAGTGTTTCTAATCTGAnAATCCAAATTAGA
178
+ AATGCTCACAGTCTGAAACTTTTTGAGCACCAACTAATGTTCAAAGAATTGCTATGGACC
179
+ ATATGGGTGGTTGATTTT
180
+
181
+ BaseQuality : 22am91h5.r1t
182
+ 4 8 4 4 4 4 4 7 10 9 10 15 10 7 7 10 10 9 19 20 33 34 34 34 36 36 33
183
+ 33 31 33 33 32 31 31 26 26 27 23 30 26 26 26 26 34 34 38 38 38 37 45
184
+ 45 37 37 37 37 45 45 45 45 37 37 37 45 45 32 18 10 21 18 28 29 33 33
185
+ 33 33 45 45 42 42 37 33 33 33 36 33 33 30 30 30 30 32 36 33 33 33 33
186
+ 33 33 37 37 45 45 37 37 37 33 33 35 35 35 35 43 35 42 42 35 35 34 34
187
+ 34 29 29 22 27 27 27 37 27 37 45 33 30 30 37 28 32 32 10 10 21 32 32
188
+ 34 27 23 12 11 9 9 9 18 21 28 31 33 30 30 30 30 34 34 34 34 31 33 34
189
+ 34 33 31 33 33 33 33 33 33 45 45 40 37 37 37 33 33 30 30 30 30 33 30
190
+ 33 26 26 26 25 23 18 14 12 20 23 27 25 21 21 21 28 28 30 31 31 31 31
191
+ 32 21 21 12 6 6 9 9 14 17 19 17 10 16 13 8 8 11 13 9 13 12 14 14 21 23
192
+ 26 34 36 34 31 22 24 20 28 28 33 27 28 25 27 24 34 32 23 12 12 12 23
193
+ 23 33 28 34 34 37 32 32 15 11 11 32 32 40 37 38 18 11 9 9 18 25 45 34
194
+ 28 28 9 9 20 29 45 38 34 23 23 10 23 23 33 33 33 21 21 13 25 25 25 29
195
+ 27 33 30 33 33 34 33 30 30 19 19 30 30 30 26 26 30 27 27 23 19 15 24
196
+ 27 28 21 9 9 9 19 14 12 10 12 12 13 17 28 18 19 21 24 24 25 24 24 24
197
+ 24 18 18 13 18 8 8 8 10 10 10 7 7 7 8 8 12 15 4 4 4 25 18 21 17 15 16
198
+ 14 9 9 17 14 17 9 17 8 8 10 10 10 11 17 17 22 24 23 24 24 19 13 10 8
199
+ 15 15 10 9 9 9 16 12 12 14 19 18 26 24 22 22 24 25 21 24 24 32 19 20 9
200
+ 9 4 4 4 4 4 4 4 4 17 16 16 4 4 4 7 10 8 7 6 13 8 8 4 4 4 4 8 8 7 7 7 4
201
+ 4 4 17 23 12 9 8 7 7 8 7 10 10 8 6 6 6 6 6 10 10 7 7 7 7 9 7 8 10 7 7
202
+ 8 6 6 8 8 8 9 10 7 12 4 4 4 9 9 7 6 6 7 6 6 10 9 7 8 10 7 7 7 9 4 4 4
203
+ 14 8 8 9 7 6 6 8 8 8 7 7 6 6 9 7 7 10 13 8 9 8 8 8 14 7 7 7 7 9 8 12
204
+ 12 4 4 4 7 7 7 7 8 8 8 7 7 7 7 7 7 8 10 8 6 6 6 4 4 4 8 12 9 9 17 9 7
205
+ 7 7 7 6 6 6 6 8 10 6 6 6 8 6 6 6 6 6 6 6 6 6 6 6 6 6 13 11 9 8 7 8 7 7
206
+ 7 8 7 9 8 7 6 6 4 6 6 8 7 7 7 6 8 6 6 8 7 7 9 9 10 11 7 6 6 6 6 6 12 9
207
+ 8 8 12 11 10 8 7 7 4 4 4 7 7 8 4
208
+
209
+
210
+ Sequence : 22am91h5.r1t
211
+ Is_read
212
+ Padded
213
+ Staden_id 20
214
+ Clipping QUAL 32 361
215
+ Align_to_SCF 1 678 1 678
216
+ SCF_File 22am91h5.r1tSCF
217
+ Primer Universal_primer
218
+ Strand Reverse
219
+ Dye Dye_terminator
220
+ Template 22am91h5
221
+ Clone bK216E10
222
+ Sequencing_vector "m13mp18"
223
+ Seq_vec SVEC 1 31 "M13mp18"
224
+ Tag ALUS 153 443
225
+
226
+ DNA : 22aq40a8.s1
227
+ AAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCACAGAGACAAGGCCAAACATGG
228
+ TAGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAGTGCGGGTGAATCACGAGGTCAG
229
+ GAGTTCAAGACCAGCCTGGCCAACATGGTGAA-CACCGTCTCTACTAAAATAAAAAGAAA
230
+ TTAGCTGGGTGTCGTGGCAGGTGCTtGTAATCCCAGCCACTTGGGAAGCTGAGGCAGGAG
231
+ AATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCGACTGCACTCCA
232
+ GCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGACAAGACAACTAG
233
+ TACAGTACTTACAGGGTTATTATGATGATTAnATGAGAGAATAGCTGTGAGGTGATTGAT
234
+ ATAGTGCTGTGCTTAAATACAACTATCAATTTTATTATACGGGGTTGAGTGTTTCCAATC
235
+ TGAAAATCCCAAATTTGGAATGCTCTACGGTCTGAAACCTTTTTTGAGCACCGACCTAAA
236
+
237
+ BaseQuality : 22aq40a8.s1
238
+ 7 9 10 11 11 23 27 25 25 9 9 9 12 14 23 27 24 21 21 16 22 22 22 24 23
239
+ 23 23 30 30 30 30 30 32 34 31 31 34 34 28 28 28 30 30 30 28 28 28 34
240
+ 34 34 34 34 34 37 34 34 34 34 34 34 37 37 37 37 37 37 37 32 34 29 29
241
+ 29 45 38 40 40 37 37 32 37 37 37 37 37 37 37 37 37 40 40 40 40 40 37
242
+ 30 30 32 22 22 22 32 32 41 45 45 41 41 45 30 32 27 32 32 40 37 37 40
243
+ 37 37 45 45 45 40 40 37 37 37 37 37 37 37 37 40 37 37 37 31 32 28 28
244
+ 28 32 32 37 34 37 38 38 38 27 27 11 1 11 10 21 27 34 34 37 37 34 34 45
245
+ 45 45 38 37 37 37 37 37 37 34 34 34 34 33 30 30 28 28 28 28 28 30 37
246
+ 37 37 37 33 36 36 31 31 31 24 24 24 24 21 21 17 12 4 4 4 14 17 26 25
247
+ 25 31 34 34 31 31 31 33 31 31 34 35 37 37 32 32 34 34 30 31 31 34 30
248
+ 33 30 30 30 33 33 30 30 23 22 22 32 32 40 34 36 34 31 26 28 26 24 16 9
249
+ 9 9 8 15 22 22 25 25 31 31 30 30 28 30 30 30 30 28 28 28 30 30 30 30
250
+ 30 37 32 27 23 18 18 27 27 34 34 31 33 30 30 28 28 28 28 28 30 34 33
251
+ 33 33 33 30 26 26 23 23 23 30 37 36 32 32 24 23 21 20 28 31 29 30 22
252
+ 21 18 13 15 23 26 34 34 36 33 30 30 30 30 25 25 25 26 23 26 25 24 24
253
+ 25 27 25 22 14 15 12 12 12 21 21 25 25 22 21 21 24 19 19 14 16 11 13
254
+ 14 20 27 30 28 28 28 30 33 34 34 23 23 16 7 7 4 4 4 18 17 21 23 21 17
255
+ 11 13 18 18 18 20 18 18 11 14 7 7 7 15 20 18 11 10 15 23 18 16 10 8 8
256
+ 13 17 18 14 14 14 13 13 14 17 18 13 13 9 9 9 14 12 18 18 11 11 5 5 5 6
257
+ 6 6 8 12 10 13 15 11 11 11 8 6 6 6 6 6 8 6 6 8 8 8 7 7 9 9 8 8 19 8 8
258
+ 14 8 8 6 6 11 9 9 8 14 6 6 7 7 8 6 7 6 6 9 9 10 15 14 12 7 7 7 7 13 15
259
+ 22 22 19 14 9 8 8 8 12 15 19 7 6 10 9 8 6 7 9 9 10 10 8 8 7 7 4 4
260
+
261
+
262
+ Sequence : 22aq40a8.s1
263
+ Is_read
264
+ Padded
265
+ Staden_id 3
266
+ Clipping QUAL 39 388
267
+ Align_to_SCF 1 152 1 152
268
+ Align_to_SCF 154 540 153 539
269
+ SCF_File 22aq40a8.s1SCF
270
+ Primer Universal_primer
271
+ Strand Forward
272
+ Dye Dye_primer
273
+ Template 22aq40a8
274
+ Clone bK216E10
275
+ Sequencing_vector "m13mp18"
276
+ Seq_vec SVEC 1 38 "M13mp18"
277
+ Tag ALUS 49 339
278
+ Tag DONE 206 206 "AUTO-EDIT: replaced N by t at 206 (terminated, compound, strong)"
279
+
280
+ DNA : 22an02b10.s1t
281
+ CGTGCCTGCAGGTCGACTCTAGAGGATCCCCCCTGTAATCCCAGCACTTTGGGAGGCCAg
282
+ TGCGGGTGAATCACgATGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACACCG
283
+ TCTCTACTAAAATAAAAAGAAATTAGCTGGGTGTCgTGGCAGGTGCCTGTAATCCCAGCC
284
+ ACTTGGGAAGCTGAgGCAGGAGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCC
285
+ GAgATCACGCGACTGCACTCCAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAA
286
+ AAAGCAGAGACAAGACAACTAGTACAGTACTTACaGGGTTATTATGATGATTAAATGAGA
287
+ gAATAGCTGTGAgGTGATTGATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATA
288
+ CGGGTTGAGTGTATCTAATCTGAAAATCCAAAATCAGAAATGCGCTACAGTCTGAAACTT
289
+ TTTTGAGCACCGACCTAATGTTCCAAGGAAGTGCTTATCGGAGCACCATGGGTCGGTACA
290
+ CATTTGGGTCGGGAATATnCCACCAGTGAGTACCATAAAATGCCAATATCCCCTCCCACC
291
+ CCCAACA
292
+
293
+ BaseQuality : 22an02b10.s1t
294
+ 7 7 9 12 10 15 16 17 13 13 21 18 23 17 28 28 37 37 37 33 26 26 17 19
295
+ 12 21 25 36 45 45 34 34 34 34 34 37 37 37 37 37 32 25 9 9 13 17 17 14
296
+ 18 18 21 29 32 19 18 18 18 21 9 9 9 23 19 30 30 37 29 37 37 32 34 23
297
+ 20 13 13 10 13 22 32 21 17 17 23 18 10 10 10 12 13 9 9 14 23 29 28 22
298
+ 22 32 33 37 34 34 34 34 34 34 34 30 37 32 37 37 41 34 33 33 33 21 21
299
+ 14 14 14 24 28 41 37 32 32 21 21 21 25 30 30 37 30 37 30 30 30 32 36
300
+ 28 27 17 27 27 37 32 37 36 37 25 23 10 10 10 32 21 23 10 15 10 23 23
301
+ 36 32 32 34 34 34 37 37 25 25 13 9 9 21 25 25 32 32 32 41 32 28 28 11
302
+ 22 22 32 32 13 13 10 11 9 10 10 12 12 14 32 32 29 22 22 21 21 17 21 27
303
+ 41 41 41 28 27 32 27 27 29 37 32 34 17 15 14 28 23 25 20 9 9 9 25 10
304
+ 12 12 14 13 10 10 9 16 13 23 23 14 14 22 25 34 28 28 22 28 28 28 19 18
305
+ 10 10 11 19 21 28 28 28 30 30 30 23 18 10 10 28 32 32 34 28 28 28 28
306
+ 28 31 34 34 25 25 12 19 11 14 14 25 21 24 24 18 18 18 22 23 13 11 10
307
+ 10 10 10 14 13 10 9 9 7 8 14 17 16 10 10 13 16 15 18 18 18 18 14 15 12
308
+ 12 9 12 9 12 12 13 9 14 10 18 19 14 9 13 11 18 14 15 15 16 20 19 18 14
309
+ 9 8 8 6 6 6 11 9 9 14 17 20 12 13 6 6 8 8 11 10 21 23 15 14 13 13 14
310
+ 21 27 28 32 29 34 28 27 23 14 10 10 12 17 15 13 11 11 11 18 28 33 24
311
+ 18 14 12 17 12 12 18 22 19 19 24 28 20 21 19 19 20 17 17 26 25 20 9 14
312
+ 7 7 7 15 21 33 27 27 18 20 19 15 15 20 18 14 14 8 8 9 9 9 7 7 7 7 7 8
313
+ 9 9 12 7 7 9 11 10 11 13 13 11 7 7 8 15 17 26 25 25 27 25 24 21 19 20
314
+ 18 13 13 14 9 9 14 18 17 12 15 16 21 20 18 8 7 6 6 6 13 20 25 18 11 14
315
+ 9 9 9 11 11 12 12 15 15 18 10 10 8 8 10 10 9 9 9 16 11 7 7 6 6 8 4 4 4
316
+ 4 4 6 10 10 13 13 12 6 6 6 6 12 15 12 11 11 4 4 4 6 6 6 6 6 8 6 6 6 8
317
+ 6 8 11 13 8 8 6 6 6 6 9 16 9 13 13 17 14 13 9 7 7 12 8 8 8 9 9 9 9 9 9
318
+ 8 8 8 10 9 7
319
+
320
+
321
+ Sequence : 22an02b10.s1t
322
+ Is_read
323
+ Padded
324
+ Staden_id 21
325
+ Clipping QUAL 32 446
326
+ Align_to_SCF 1 607 1 607
327
+ SCF_File 22an02b10.s1tSCF
328
+ Primer Universal_primer
329
+ Strand Forward
330
+ Dye Dye_terminator
331
+ Template 22an02b10
332
+ Clone bK216E10
333
+ Sequencing_vector "m13mp18"
334
+ Seq_vec SVEC 1 31 "M13mp18"
335
+ Tag ALUS 32 301
336
+ Tag DONE 60 60 "AUTO-EDIT: inserted g at 60 (terminated, compound, strong)"
337
+ Tag DONE 75 75 "AUTO-EDIT: replaced A by g at 75 (terminated, isolated, strong)"
338
+ Tag DONE 156 156 "AUTO-EDIT: replaced C by g at 156 (terminated, isolated, strong)"
339
+ Tag DONE 195 195 "AUTO-EDIT: replaced A by g at 195 (terminated, isolated, strong)"
340
+ Tag DONE 243 243 "AUTO-EDIT: replaced T by g at 243 (terminated, isolated, strong)"
341
+ Tag DONE 335 335 "AUTO-EDIT: replaced T by a at 335 (all_stranded, isolated, strong)"
342
+ Tag DONE 361 361 "AUTO-EDIT: replaced C by g at 361 (terminated, isolated, strong)"
343
+ Tag DONE 373 373 "AUTO-EDIT: replaced C by g at 373 (terminated, isolated, strong)"
344
+
345
+ DNA : 22ak67d6.r1t
346
+ CGCGCCCCTCnGACTCCGGCCCTAnCACGGTACCCACTTTGGGAGGCCAGTGCGGGTGAA
347
+ TCACGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACACCGTCTCTACTAA
348
+ AATAAAAAGAAATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAGCCACTTGGGAAG
349
+ CTGAgGCAGGAGAATCGCTTGAACCCGGGAgGTGGAGGTTGCAGTGAGCCGAGATCACGC
350
+ GACTGCACTCCAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGA
351
+ TCAGATCACTAGTACAGTACTTACGGGCTTATTATGATGATTAAATGAGAGAATACCTGT
352
+ GAGGTGATTGATATATTGCTGTGCTTAATACAnACTATCATTTTATTATACGGGTCGAGT
353
+ GTTTCTAATCTGACAATCCAAATTAGAAATGCTCTACAGTCTGAAACCTTTTTGAGCACC
354
+ CACCTACTGTTCAAAGGACGTGCTTATTGGAGCATTATGGGTTGTAACATTTTTGGGTTG
355
+ GGAATTTTCACCCATTAGTACTATAAATGCCATTTTCCAAAAAATCTGAATCTAAAATTT
356
+ CTGGTCCTACCAGCATTTGCTAGGGATCCCACCCGTCTTCTTCCTTACCTGTTTAAGTCT
357
+ TATAAATC
358
+
359
+ BaseQuality : 22ak67d6.r1t
360
+ 6 6 6 4 8 11 15 8 4 4 4 4 8 6 6 9 7 8 9 8 4 4 4 4 4 4 10 9 9 21 19 33
361
+ 30 33 30 30 30 28 28 28 23 23 20 11 11 16 12 13 17 21 25 28 33 33 33
362
+ 38 34 34 34 33 30 22 22 12 14 21 10 12 21 21 16 14 11 13 13 14 21 21
363
+ 21 19 15 18 23 23 25 30 28 33 38 38 33 33 30 30 30 30 30 30 30 37 37
364
+ 45 45 33 33 33 33 33 33 40 42 49 42 40 40 40 34 34 31 35 34 35 37 42
365
+ 42 37 32 19 9 9 11 23 19 28 30 30 28 38 45 37 37 37 32 32 24 34 34 30
366
+ 30 26 21 20 11 27 27 40 34 30 30 28 30 30 30 23 18 17 9 9 18 16 16 24
367
+ 24 24 23 21 18 11 11 18 25 25 16 9 7 9 15 11 8 7 9 7 7 8 16 16 27 28
368
+ 25 20 16 10 10 9 13 8 8 7 12 4 4 4 14 6 6 6 7 9 6 7 7 7 7 16 10 20 15
369
+ 13 11 9 9 8 8 8 15 8 10 10 17 14 9 9 19 15 15 7 7 12 20 19 25 25 22 18
370
+ 17 7 9 12 20 21 25 21 21 20 22 28 32 30 25 25 12 12 18 32 32 36 49 49
371
+ 33 33 24 20 13 12 16 18 20 14 25 23 25 19 12 11 8 8 4 4 4 4 4 8 8 6 4
372
+ 6 8 15 17 21 19 15 8 8 8 8 10 7 7 13 9 7 11 14 6 6 4 4 4 6 12 8 13 17
373
+ 21 18 19 19 20 18 18 15 10 15 14 13 14 8 8 8 9 8 8 10 10 8 8 15 17 17
374
+ 17 17 14 13 19 17 16 12 10 14 15 17 15 14 8 8 7 7 15 19 18 12 11 16 12
375
+ 15 11 10 13 13 11 4 4 4 10 12 12 18 12 14 9 10 9 10 21 22 18 14 6 6 6
376
+ 6 6 8 4 4 4 8 8 7 8 7 9 14 7 8 7 10 16 31 17 14 4 4 4 10 11 8 10 13 17
377
+ 13 8 8 9 8 8 12 12 14 14 7 7 7 7 7 7 7 8 9 13 11 10 7 10 7 6 6 10 8 8
378
+ 23 23 12 7 7 7 7 7 10 10 18 6 6 6 6 6 6 7 7 7 6 6 6 7 6 6 4 4 4 4 8 11
379
+ 18 15 23 11 9 8 7 7 7 7 7 7 8 15 18 8 10 10 12 8 6 8 6 6 6 7 7 12 10
380
+ 12 18 11 12 10 19 19 15 15 10 10 9 7 11 7 8 9 10 7 7 6 6 6 6 4 6 7 7 7
381
+ 7 9 9 9 13 13 9 7 7 7 9 7 7 7 6 6 6 6 8 8 9 8 11 8 7 6 6 6 6 9 7 8 7 7
382
+ 6 8 6 6 9 10 12 10 13 12 6 6 6 6 6 7 6 6 7 7 7 11 13 11 9 8 6 6 8 6 6
383
+ 8 12 14 8 8 8 6 13 8 8 8 6 7 8 7 8 9 8 10 6 6 10 11 17 20 16 11 11 7 6
384
+ 8 8 9 8 6 12 10 7 7 8 8 4
385
+
386
+
387
+ Sequence : 22ak67d6.r1t
388
+ Is_read
389
+ Padded
390
+ Staden_id 19
391
+ Clipping QUAL 30 292
392
+ Align_to_SCF 1 668 1 668
393
+ SCF_File 22ak67d6.r1tSCF
394
+ Primer Universal_primer
395
+ Strand Reverse
396
+ Dye Dye_terminator
397
+ Template 22ak67d6
398
+ Clone bK216E10
399
+ Sequencing_vector "m13mp18"
400
+ Tag ALUS 35 291
401
+ Tag DONE 185 185 "AUTO-EDIT: replaced C by g at 185 (terminated, isolated, strong)"
402
+ Tag DONE 211 211 "AUTO-EDIT: replaced T by g at 211 (terminated, isolated, strong)"
403
+
404
+ DNA : 22ak64b1.s1t
405
+ TGGGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAACACCGTCTCTACTAAA
406
+ ATAAAAAGAAATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAGCCACTTGGGAAGC
407
+ TGAGGCAGGAGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAgATCACGCG
408
+ ACTGCACTCCAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGAC
409
+ AAGAcCACTAGTACAGTACTTACAGGGTtATTATGATGATTAAATGAGAGAATAGCTGTG
410
+ AGGTGATTGATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATACGGGTTGAGTG
411
+ TTTCTAATCTGAaAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACC
412
+ GACCTAAATGTTCAAAGGGAGGTGCTTAATTGGAGCATTATGGGTTGTTAGATCTTTGGG
413
+ TTGGGAATATTCAACC
414
+
415
+ BaseQuality : 22ak64b1.s1t
416
+ 4 4 8 11 13 20 16 16 16 19 24 32 31 37 37 35 34 29 32 18 32 24 33 33
417
+ 37 37 37 23 32 11 10 21 17 37 34 34 45 45 45 45 32 29 26 26 28 30 30
418
+ 33 45 45 49 49 45 43 45 45 45 45 45 37 45 45 45 45 45 45 45 37 37 37
419
+ 38 32 40 37 41 37 45 32 45 45 44 37 45 30 45 45 33 33 33 33 33 22 37
420
+ 37 45 45 45 40 37 34 37 37 33 32 32 33 33 33 33 40 40 40 42 40 42 37
421
+ 37 37 45 45 45 45 45 35 34 34 28 28 18 21 17 17 27 38 38 45 34 34 34
422
+ 34 34 34 34 34 34 34 34 34 34 34 34 37 34 34 33 33 33 33 28 31 31 36
423
+ 34 31 36 36 34 33 20 14 8 8 8 8 14 23 27 31 36 33 36 38 30 30 28 28 28
424
+ 30 30 27 30 30 30 30 33 33 23 21 18 18 17 20 32 37 37 45 45 45 45 34
425
+ 34 34 45 45 45 44 45 37 45 45 45 37 40 37 37 33 40 44 40 40 36 31 31
426
+ 28 25 22 27 14 14 8 9 10 8 7 8 7 12 18 18 23 15 9 9 15 15 20 20 25 28
427
+ 25 21 21 18 18 8 9 8 6 6 8 11 14 28 28 28 30 30 30 30 31 30 31 25 22
428
+ 22 27 15 15 15 16 13 17 25 25 30 25 27 28 31 24 24 19 24 29 33 27 31
429
+ 31 31 31 28 27 22 14 15 27 32 40 37 36 36 36 33 33 34 33 27 13 14 7 7
430
+ 8 18 23 32 32 23 20 20 33 30 27 27 21 14 14 18 21 18 21 15 15 17 11 11
431
+ 16 24 23 30 23 14 10 13 14 15 10 8 9 16 16 28 4 4 4 10 11 12 9 8 10 14
432
+ 20 18 19 19 19 10 10 12 15 25 23 26 26 28 18 14 14 16 20 15 14 12 12
433
+ 12 21 23 25 24 21 17 17 14 21 20 19 12 9 9 8 9 8 10 7 6 9 8 7 8 9 7 7
434
+ 7 6 6 8 7 8 6 6 6 6 6 13 11 6 6 6 8 10 11 8 9 9 10 10 15 10 11 12 16
435
+ 13 10 9 7 7 7 7 9 10 8 8 7 6 6 6 6 6 14 10 7 7 7 9 9 10 11 16 16 7 6 4
436
+ 4 4 8 4
437
+
438
+
439
+ Sequence : 22ak64b1.s1t
440
+ Is_read
441
+ Padded
442
+ Staden_id 8
443
+ Clipping QUAL 40 416
444
+ Align_to_SCF 1 496 1 496
445
+ SCF_File 22ak64b1.s1tSCF
446
+ Primer Universal_primer
447
+ Strand Forward
448
+ Dye Dye_terminator
449
+ Template 22ak64b1
450
+ Clone bK216E10
451
+ Sequencing_vector "m13mp18"
452
+ Seq_vec SVEC 1 39 "M13mp"
453
+ Tag CONF 44 44 "AUTOEDIT: possible compression"
454
+ Tag CONF 96 96 "AUTOEDIT: possible compression"
455
+ Tag DONE 172 172 "AUTO-EDIT: replaced A by g at 172 (terminated, isolated, strong)"
456
+ Tag DONE 245 245 "AUTO-EDIT: replaced A by c at 245 (all_stranded, compound, strong)"
457
+ Tag AMBG 246 246 "AUTOEDIT: Check this edit stack (conflict & possible misassembly))!"
458
+ Tag DONE 269 269 "AUTO-EDIT: replaced C by t at 269 (all_stranded, isolated, strong)"
459
+ Tag DONE 373 373 "AUTO-EDIT: replaced N by a at 373 (terminated, isolated, strong)"
460
+
461
+ DNA : 22ak81h10.s1t
462
+ GTGGCATGCCTGCAGGTCAACTCTAAAAGATCCCCTGAAACACCGTCTCTACTAAAATAA
463
+ AAAGAAATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAgCCACTTGGGAAGCTGAG
464
+ GCAGGAGAATCGCTTGAACCCGGGAgGTGGAgGTTGCAGTGAGCCGAGATCACGCGACTG
465
+ CACTCCAGCCCAAcCAATAGTGTGAGACTCTGTCTCGAaaAAaAAAAAGCAgAGACaAGA
466
+ CCACTAGTACAGTACTTACAGGGTTATTATGATGATTAAaTGAGAGAATAGCTGTGAGGT
467
+ GATTGATATAGTGCTGTGCTTAATACaAACTATCATTTTATTATACGGGTTGAGTGTTTC
468
+ TAATCTGAAAATCCaaAATTAGAAATGCTCTACAGTCTGAAACTTTtTTGAGCACCGACC
469
+ TACTGTGCACAGGAAGTGCTTATTGGAGCATGATGGGTTGTTACATATTTGGGTTGGGAA
470
+ TATTCACCCAGTAAGTACTATAAAATGCAAATATTCCAACAAG
471
+
472
+ BaseQuality : 22ak81h10.s1t
473
+ 6 6 6 6 6 8 9 16 11 13 21 20 22 22 22 15 14 9 9 9 9 15 18 15 9 9 9 9 9
474
+ 15 23 36 36 36 36 34 38 40 30 28 28 28 30 32 37 37 36 38 37 44 45 45
475
+ 49 49 45 45 37 37 38 45 45 45 38 37 37 38 38 37 37 34 34 34 32 32 38
476
+ 45 49 45 45 45 45 37 33 33 27 27 18 18 30 32 36 36 36 36 36 36 36 34
477
+ 32 21 18 4 4 4 18 17 27 28 33 36 36 36 49 36 36 36 34 34 45 27 27 30
478
+ 25 27 12 20 20 34 34 38 37 37 34 34 25 25 18 18 21 29 38 45 21 28 14
479
+ 14 14 27 21 34 18 17 17 32 27 25 22 13 13 10 18 15 32 32 32 37 28 22
480
+ 22 21 14 10 10 11 23 22 28 20 14 14 8 8 11 20 20 16 12 16 16 28 21 19
481
+ 10 10 10 19 18 20 20 14 14 32 32 34 32 25 18 18 18 18 27 32 32 24 24
482
+ 23 23 14 10 10 10 9 9 10 11 12 12 12 12 17 11 11 11 10 8 6 6 8 8 10 16
483
+ 16 16 11 14 10 9 9 9 17 11 11 11 14 15 22 18 14 14 11 11 15 27 22 19
484
+ 23 27 30 28 22 18 16 11 10 10 11 9 10 11 11 12 15 20 27 27 23 21 14 10
485
+ 10 10 10 14 22 25 22 14 14 22 32 38 34 34 34 31 24 18 14 12 9 10 10 23
486
+ 18 24 16 16 18 18 18 19 18 17 21 18 18 12 14 21 27 37 34 34 28 28 34
487
+ 27 27 28 31 20 18 11 9 9 12 11 11 27 15 15 20 23 26 14 8 8 8 8 14 16
488
+ 23 19 19 28 31 31 31 25 18 11 11 18 10 10 10 10 11 12 16 16 21 23 12
489
+ 12 13 23 13 13 19 11 10 10 10 10 10 18 21 28 16 19 29 32 19 19 8 8 8
490
+ 10 10 18 18 22 21 16 18 22 21 11 11 14 14 15 14 18 7 7 7 7 7 7 14 18
491
+ 10 10 9 9 14 15 15 23 20 22 19 19 21 16 11 11 9 8 8 9 9 18 14 11 9 9
492
+ 15 8 9 10 8 9 8 8 8 14 14 25 27 16 9 8 14 8 9 17 13 18 15 8 8 7 7 7 7
493
+ 6 6 6 6 6 4 4 4 8 9 10 17 11 15 12 17 9 9 10 7 7 7 10 11 10 7 7 7 6 6
494
+ 8 6 6 8 4
495
+
496
+
497
+ Sequence : 22ak81h10.s1t
498
+ Is_read
499
+ Padded
500
+ Staden_id 5
501
+ Clipping QUAL 36 418
502
+ Align_to_SCF 1 523 1 523
503
+ SCF_File 22ak81h10.s1tSCF
504
+ Primer Universal_primer
505
+ Strand Forward
506
+ Dye Dye_terminator
507
+ Template 22ak81h10
508
+ Clone bK216E10
509
+ Sequencing_vector "m13mp18"
510
+ Seq_vec SVEC 1 35 "M13mp1"
511
+ Tag ALUS 36 216
512
+ Tag DONE 103 103 "AUTO-EDIT: replaced N by g at 103 (terminated, isolated, strong)"
513
+ Tag DONE 146 146 "AUTO-EDIT: replaced T by g at 146 (terminated, isolated, strong)"
514
+ Tag DONE 152 152 "AUTO-EDIT: replaced A by g at 152 (terminated, isolated, strong)"
515
+ Tag DONE 194 194 "AUTO-EDIT: replaced G by c at 194 (all_stranded, isolated, strong)"
516
+ Tag DONE 219 219 "AUTO-EDIT: replaced G by a at 219 (all_stranded, compound, strong)"
517
+ Tag DONE 220 220 "AUTO-EDIT: replaced C by a at 220 (all_stranded, compound, strong)"
518
+ Tag DONE 223 223 "AUTO-EDIT: replaced G by a at 223 (all_stranded, isolated, strong)"
519
+ Tag DONE 232 232 "AUTO-EDIT: replaced C by g at 232 (all_stranded, isolated, strong)"
520
+ Tag DONE 237 237 "AUTO-EDIT: replaced G by a at 237 (all_stranded, isolated, strong)"
521
+ Tag DONE 280 280 "AUTO-EDIT: replaced G by a at 280 (terminated, isolated, strong)"
522
+ Tag DONE 327 327 "AUTO-EDIT: replaced G by a at 327 (terminated, isolated, strong)"
523
+ Tag DONE 375 375 "AUTO-EDIT: replaced T by a at 375 (terminated, compound, strong)"
524
+ Tag DONE 376 376 "AUTO-EDIT: replaced C by a at 376 (terminated, compound, strong)"
525
+ Tag DONE 407 407 "AUTO-EDIT: replaced C by t at 407 (terminated, isolated, strong)"
526
+
527
+ DNA : 22ak66c6.s1t
528
+ nCGTGGCTGCCTGCAGGTCGACTCTAGAGGATCCCCACCGTCTCTACTAAAATAAAAAGA
529
+ AATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAGCCACTTGGGAAGCTGAGGCAGG
530
+ AGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCGACTGCACTC
531
+ CAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGACAAGACAACT
532
+ AGTACAGTACTTACAGGGTTATTATGATGATTAAATGAGAGAATAGCTGTGAGGTGATTG
533
+ ATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATACGGGTTGAGTGTTTCTAATC
534
+ TGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAATG
535
+ TTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGGGTTGGGAATATTC
536
+ AACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAATCTGAAACATtTC
537
+ TGGTCCTAAGCAAGCATnTTGCAAAGGGATACGCAACCTGTAGTACGTCCTTTATCATTG
538
+ TnTCAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAnCAnATTCGATTGTA
539
+ TTATT
540
+
541
+ BaseQuality : 22ak66c6.s1t
542
+ 4 4 8 4 6 10 13 21 24 25 33 33 33 30 27 21 15 27 19 30 30 33 33 30 21
543
+ 21 10 9 17 11 27 27 37 38 33 35 35 35 35 44 45 45 38 37 37 45 45 45 43
544
+ 43 43 45 45 45 45 45 45 45 21 21 23 30 30 34 37 37 38 45 32 45 37 41
545
+ 37 45 30 45 45 34 34 34 32 29 22 32 32 45 45 45 33 28 28 37 37 34 34
546
+ 34 35 34 34 34 34 34 34 37 37 40 41 37 34 37 37 37 32 24 22 27 29 25
547
+ 27 20 21 21 21 27 45 41 40 40 40 42 34 34 37 41 45 51 45 45 37 30 37
548
+ 41 41 37 36 28 30 30 30 22 33 33 35 33 33 41 51 51 45 39 39 39 30 28
549
+ 33 34 34 39 34 41 34 34 41 33 33 39 39 39 33 33 33 30 33 33 33 30 29
550
+ 19 19 24 25 32 33 45 36 36 36 36 41 41 30 30 30 37 37 43 51 51 51 51
551
+ 45 37 37 30 37 37 37 51 51 51 51 51 37 37 28 28 10 10 10 13 10 10 10 9
552
+ 9 9 21 21 28 37 37 37 33 33 33 33 33 33 34 34 34 33 33 33 33 21 25 25
553
+ 22 22 29 29 33 33 33 33 31 31 27 28 17 23 23 28 26 24 32 10 10 10 15
554
+ 15 26 32 32 37 45 45 32 45 33 26 24 32 37 37 35 34 37 35 37 34 37 37
555
+ 40 37 37 45 45 38 38 45 45 40 37 37 37 34 30 30 28 28 28 30 37 37 34
556
+ 34 34 34 34 33 31 31 25 21 19 22 21 25 30 32 32 30 22 22 25 28 24 25
557
+ 26 17 17 23 27 27 33 31 31 34 38 38 38 38 34 26 17 15 8 8 11 16 25 31
558
+ 32 33 29 26 32 29 33 34 34 34 36 33 34 34 31 27 31 34 38 45 45 36 37
559
+ 37 37 36 36 38 34 32 28 25 22 22 21 17 18 29 29 23 23 22 27 25 20 18
560
+ 11 11 15 15 16 23 21 17 19 24 24 24 31 31 31 33 33 33 31 33 33 29 29
561
+ 29 29 29 31 31 31 23 24 19 12 10 16 10 10 20 10 10 13 15 30 23 29 23
562
+ 28 18 10 10 16 21 18 19 19 24 24 12 11 9 9 10 21 23 31 31 33 28 28 14
563
+ 17 17 28 21 30 24 30 28 22 26 23 19 10 10 12 23 22 23 19 12 10 9 9 10
564
+ 19 24 29 30 34 34 34 34 34 29 25 25 32 26 31 31 32 20 17 15 12 12 4 4
565
+ 4 17 23 33 30 24 18 13 18 16 12 16 8 9 9 19 15 15 4 4 4 7 11 6 6 6 7 8
566
+ 9 17 12 13 19 23 25 13 13 13 19 21 29 32 30 26 20 20 12 9 9 8 9 8 8 15
567
+ 24 17 15 8 8 9 17 16 4 4 4 8 8 13 15 23 14 9 8 8 10 10 17 23 21 17 12
568
+ 13 22 20 19 15 11 11 9 8 8 8 8 8 8 10 9 9 7 8 8 10 12 10 10 12 4 4 4 4
569
+ 4 4 10 10 10 10 10 9 8 8 7 8 9 11 9 9 4
570
+
571
+
572
+ Sequence : 22ak66c6.s1t
573
+ Is_read
574
+ Padded
575
+ Staden_id 18
576
+ Clipping QUAL 37 548
577
+ Align_to_SCF 1 665 1 665
578
+ SCF_File 22ak66c6.s1tSCF
579
+ Primer Universal_primer
580
+ Strand Forward
581
+ Dye Dye_terminator
582
+ Template 22ak66c6
583
+ Clone bK216E10
584
+ Sequencing_vector "m13mp18"
585
+ Seq_vec SVEC 1 36 "M13mp"
586
+ Tag ALUS 38 221
587
+ Tag DONE 538 538 "AUTO-EDIT: replaced N by t at 538 (terminated, isolated, strong)"
588
+
589
+ DNA : 22ak93c1.s1lt
590
+ TTTCTTTGGGGGCAATCTTGATGCTAGCGTCGTTCAAGATGATCCACACCCAGCTCACTG
591
+ TTTTATTCTCTCCAATAATCCTAAGCAAGCTCTCAAAATATAAATATGCTTTTTAATAAT
592
+ ACAATCGAATTTGCTATACCACCTCAGAATCTGTACCACAATATATTAACTACTTAAAAC
593
+ AATGATAAAGAACGTACTACAGGTTGCGTATCCCTTTGCAAAATGCTTGCTTAGGACCAG
594
+ AAATGTTTCAGATTTCAGATTTTTTTTGGAATATTTGCATTTTATAGTACTTACTGGTTG
595
+ AATATTCCCAACCCAAAAATCTAACAACCCATAATGCTCCAATAAGCACTTCCTTTGAAC
596
+ ATTAGGTCGGTGCTCAAAAAAGTTTCAGACTGTAGAGCATTTCTAATTTTGGATTTTCAG
597
+ ATTAGAAACACTCAACCCGTATAATAAAATGATAGTTTGTATTAAGCACAGCACTATATC
598
+ AATCACCTCACAGCTATTCTCTCATTTAATCATCATAATAACCCTGTAAGTACTGTACTA
599
+ gTTGTCTTGTCTCTGCTTTTTTTTTTTCgAgACAGAGTCTCACACTATTGGTTGGGCTGG
600
+ AATGCAnTCGCGTGATCTCGGCTCACTGGCAACCTCCACCTCCCGGGG
601
+
602
+ BaseQuality : 22ak93c1.s1lt
603
+ 4 4 4 4 4 4 4 4 9 6 4 8 15 11 4 6 4 12 8 9 10 12 11 8 8 6 7 8 14 14 9
604
+ 4 4 8 8 8 8 8 4 6 8 11 9 10 8 9 9 11 10 9 9 9 9 9 9 16 16 32 23 29 21
605
+ 27 27 22 22 20 28 32 37 37 34 31 30 28 28 24 29 29 34 34 38 32 32 18
606
+ 22 18 22 22 28 29 37 37 37 37 35 35 34 34 35 34 37 37 37 37 37 37 34
607
+ 37 37 37 37 32 32 28 28 31 30 30 30 23 23 23 28 27 21 32 13 13 16 24
608
+ 32 34 31 30 28 28 28 28 28 34 34 34 34 33 25 16 9 9 10 23 32 33 41 37
609
+ 34 28 30 30 30 28 28 22 27 25 32 25 30 27 22 24 22 22 28 25 24 30 30
610
+ 30 30 30 30 24 28 13 10 10 23 20 18 9 9 9 18 23 30 30 30 28 24 19 25
611
+ 11 11 12 27 31 34 30 30 30 30 37 45 51 41 39 28 28 28 25 25 24 30 34
612
+ 39 39 39 39 39 39 36 31 13 10 9 10 10 23 23 9 9 13 23 31 31 14 14 31
613
+ 31 13 9 9 9 23 23 23 13 9 9 9 31 31 41 51 36 31 14 13 31 19 30 31 31
614
+ 22 34 25 33 33 33 30 24 29 30 18 24 24 11 11 24 24 33 39 34 34 30 30
615
+ 34 22 22 14 19 18 23 23 17 31 24 37 33 33 33 33 33 28 34 18 18 27 34
616
+ 34 38 27 20 20 24 24 30 30 39 34 34 30 22 22 24 33 33 33 33 28 24 24
617
+ 22 30 22 24 24 33 33 33 39 51 51 41 32 27 17 25 17 31 31 34 19 15 10
618
+ 10 31 14 27 21 30 30 24 24 30 34 39 39 41 36 34 30 30 30 34 31 16 9 9
619
+ 10 20 19 16 11 9 9 9 13 23 22 28 28 37 33 33 33 28 32 18 13 10 20 18
620
+ 10 32 21 31 23 14 9 9 9 16 11 9 9 10 20 25 33 30 30 28 32 28 28 37 35
621
+ 35 34 34 34 34 34 37 40 37 33 32 25 12 10 10 16 16 28 32 32 34 30 28
622
+ 28 29 31 33 32 28 30 30 25 21 16 19 19 19 27 19 14 14 22 22 28 25 29
623
+ 29 29 30 30 23 23 21 18 14 21 17 24 19 32 32 37 37 31 30 30 28 31 30
624
+ 34 34 28 28 28 28 31 31 30 28 28 28 30 30 25 32 10 10 10 23 23 27 17
625
+ 17 22 17 23 23 23 24 20 17 4 4 4 14 16 24 22 19 18 13 19 9 12 14 25 25
626
+ 33 32 29 40 38 49 49 49 34 34 17 14 8 7 7 9 9 10 8 7 7 9 10 15 17 18
627
+ 22 24 18 18 18 22 20 24 24 28 18 19 18 19 21 24 25 14 12 8 8 9 14 12 4
628
+ 4 4 17 17 17 14 9 9 9 12 21 21 18 12 10 13 10 11 9 12 12 8 7 8 8 9 10
629
+ 9 15 10 8 9 10 10 21 21 28 27 18 14 7 7
630
+
631
+
632
+ Sequence : 22ak93c1.s1lt
633
+ Is_read
634
+ Padded
635
+ Staden_id 1
636
+ Clipping QUAL 56 598
637
+ Align_to_SCF 1 648 1 648
638
+ SCF_File 22ak93c1.s1ltSCF
639
+ Primer Universal_primer
640
+ Strand Forward
641
+ Dye Dye_primer
642
+ Template 22ak93c1
643
+ Clone bK216E10
644
+ Sequencing_vector "m13mp18"
645
+ Seq_vec SVEC 1 47 "M13mp18"
646
+ Tag DONE 541 541 "AUTO-EDIT: replaced N by g at 541 (all_stranded, isolated, strong)"
647
+ Tag ALUS 559 647
648
+ Tag DONE 569 569 "AUTO-EDIT: replaced A by g at 569 (all_stranded, isolated, strong)"
649
+ Tag DONE 571 571 "AUTO-EDIT: replaced A by g at 571 (all_stranded, isolated, strong)"
650
+
651
+ DNA : 22ak64e2.s1t
652
+ GTAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTGCAAAATGCTTGCTTA
653
+ GGACCAGAAATGTTTCAGATTTCAGATTTTTTTTGGAATATTTGCATTTTATAGTACTTA
654
+ CTGGTTGAATATTCCCAACCCAAAAATCTAACAACCCATAATGCTCCAATAAGCACTTCC
655
+ TTTGAACATTAGGTCGGTGCTCAAAAAAGTTTCAGACTGTAGAGCATTTCTAATTTTGGA
656
+ TTTTCAGATTAGAAACACTCAACCCGTATAATAAAATGATAGTTTGTATTAAGCACAGCA
657
+ CTATATCAATCACCTCACAGCTATTCTCTCATTTAATCATCATAATAACCCTGTAAGTAC
658
+ TGTACTAGTTGTCTTGTCTCTGCTTTTTTTTTTTCGAGACAGAGTCTCACACTATTGGTT
659
+ GGACTGGAGTGCAATTCnCCTGAATTCGGCTCACGGCAACTTCAACCTCCCGGGTTCAAG
660
+ TGAATCTCCTC
661
+
662
+ BaseQuality : 22ak64e2.s1t
663
+ 4 4 8 10 12 15 18 18 28 30 30 30 30 26 37 34 30 30 22 22 20 23 16 11
664
+ 11 18 23 23 23 28 9 9 10 9 21 12 32 34 45 45 45 45 38 30 30 30 30 30
665
+ 28 34 30 33 33 33 33 33 43 45 45 32 18 10 11 11 25 23 10 10 22 32 38
666
+ 37 27 27 27 23 20 9 9 10 23 25 25 21 9 9 10 29 32 45 45 45 45 45 45 37
667
+ 37 37 38 45 37 41 28 29 28 28 28 28 34 34 33 33 33 28 28 34 33 34 34
668
+ 34 34 34 34 45 37 37 29 37 37 45 45 45 45 42 37 34 35 35 35 34 34 34
669
+ 34 34 37 37 37 45 45 35 35 35 35 35 35 35 37 37 37 37 45 37 42 42 33
670
+ 30 30 33 33 33 40 34 34 34 34 34 34 40 40 40 37 37 37 34 34 34 34 34
671
+ 31 31 27 28 19 21 19 28 28 30 32 34 34 31 31 31 33 39 27 20 7 7 8 8 14
672
+ 15 11 11 17 28 23 28 17 9 9 11 23 27 33 33 40 40 40 40 40 40 40 37 32
673
+ 32 31 31 28 37 30 33 25 21 13 13 14 26 22 11 11 21 21 30 33 31 31 33
674
+ 37 37 37 45 49 45 40 40 40 40 40 45 40 40 40 40 40 31 29 29 32 27 37
675
+ 37 45 45 45 40 37 37 37 37 37 33 34 33 33 30 30 34 34 36 36 36 30 30
676
+ 28 28 28 27 38 38 36 31 33 32 27 20 20 20 28 32 33 24 24 29 36 34 30
677
+ 33 30 26 26 26 30 30 26 37 36 34 33 30 31 31 28 27 27 25 24 29 33 27
678
+ 27 31 31 31 31 33 36 38 38 32 32 21 13 13 16 19 19 24 25 33 30 33 33
679
+ 33 38 39 40 44 40 40 36 33 37 40 40 40 40 49 40 36 33 31 26 23 23 21
680
+ 18 20 14 12 11 14 17 25 31 33 33 33 36 30 30 30 30 30 28 28 20 15 14 4
681
+ 4 4 12 9 9 4 4 4 7 8 7 7 7 7 4 4 4 7 8 10 7 6 9 9 9 7 7 11 17 19 9 8 7
682
+ 7 8 7 8 9 7 6 10 6 7 6 6 7 7 8 8 8 12 13 8 8 8 8 10 10 9 8 7 6 6 8 13
683
+ 7 7 7 7
684
+
685
+
686
+ Sequence : 22ak64e2.s1t
687
+ Is_read
688
+ Padded
689
+ Staden_id 14
690
+ Clipping QUAL 41 420
691
+ Align_to_SCF 1 491 1 491
692
+ SCF_File 22ak64e2.s1tSCF
693
+ Primer Universal_primer
694
+ Strand Forward
695
+ Dye Dye_terminator
696
+ Template 22ak64e2
697
+ Clone bK216E10
698
+ Sequencing_vector "m13mp18"
699
+ Seq_vec SVEC 1 40 "M13mp"
700
+
701
+ DNA : 22ak70f10.s1t
702
+ TTGGCTGCCTGCAGGTCGACTCTAGAGGATCCCCCAGGGTTATTATGATGATTAAATGAG
703
+ AGAATAGCTGTGAGGTGATTGATATAGTGCTGTGCTTAATACAAACTATCATTTTATTAT
704
+ ACGGGTTGAGTGTTTCTAATCTGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACT
705
+ TTTTTGAGCACCGACCTAATGTTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAG
706
+ ATTTTTGGGTTGGGAATATTCAACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAA
707
+ ATCTGAAATCTGAAACATTTCTGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCT
708
+ GTAGTACGTTCTTTATCATTGTTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGT
709
+ GGTATAGCAAATTCGATTGTATTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAG
710
+ GATTATTGGAGAGAATAAAACAGTGAAGCTTTGGTGTTATGAGGGAATnTTAGATAGAAA
711
+ AGTGCAGTnTTTCAGTTCATGCTCTnTCATTTnTTACTCCCTCCGGTTAAAGCTGAAGCT
712
+ CAACAAAGATATAGTGATCTCTGTGGGCATTATAATCCTGTCCC
713
+
714
+ BaseQuality : 22ak70f10.s1t
715
+ 4 4 6 8 8 9 19 23 33 33 34 33 27 21 18 27 18 27 27 34 34 31 27 23 13
716
+ 11 19 16 31 31 37 45 45 34 34 31 31 22 31 25 31 37 45 45 45 45 37 37
717
+ 37 37 37 37 45 37 37 37 45 38 29 27 27 32 32 37 45 33 37 37 45 37 45
718
+ 45 37 37 37 40 40 40 37 45 37 37 34 33 33 34 34 34 37 33 40 37 37 37
719
+ 33 33 33 33 33 33 33 33 33 32 33 41 41 35 35 35 40 40 36 36 36 34 33
720
+ 33 37 37 37 37 37 41 45 30 30 41 41 37 28 45 18 18 29 35 37 37 37 37
721
+ 41 41 51 51 51 51 51 33 33 33 33 33 33 45 45 45 41 39 39 51 51 41 45
722
+ 45 45 45 35 33 33 33 33 33 40 51 51 51 51 51 51 51 51 51 43 45 39 34
723
+ 30 30 33 33 33 33 45 45 38 38 38 38 33 30 29 14 15 27 27 30 33 51 51
724
+ 41 41 37 34 37 37 37 40 40 40 37 45 51 33 33 33 33 33 33 37 37 31 45
725
+ 33 25 22 27 21 28 33 28 28 33 41 37 45 41 33 45 33 22 22 33 33 34 44
726
+ 44 45 45 33 33 33 33 33 30 34 34 34 34 37 37 29 34 37 33 33 33 33 33
727
+ 33 34 40 33 33 33 30 30 30 37 37 37 30 30 33 30 33 33 49 49 45 45 45
728
+ 45 45 45 44 38 38 38 44 38 45 45 49 40 40 35 35 35 35 38 37 40 37 37
729
+ 34 34 34 34 34 34 34 36 36 36 34 36 33 31 31 36 33 28 28 27 23 19 17
730
+ 17 23 23 23 33 27 28 28 28 30 33 40 40 31 31 17 20 20 28 32 25 17 17
731
+ 32 27 37 37 36 33 33 33 33 32 36 34 28 30 32 31 31 14 18 10 9 9 19 21
732
+ 30 31 38 38 38 37 37 31 34 34 36 31 30 31 31 31 31 33 33 33 39 30 25
733
+ 24 24 29 33 33 34 33 33 23 23 12 23 23 30 33 33 37 37 37 38 40 37 34
734
+ 33 37 36 33 33 33 33 33 33 36 28 28 22 24 24 24 21 29 33 33 33 34 33
735
+ 33 33 33 33 33 33 33 33 27 28 28 31 31 23 27 19 15 11 20 21 33 33 31
736
+ 31 31 31 31 26 28 24 24 24 29 24 29 29 33 27 31 27 30 36 29 18 20 12
737
+ 17 9 9 9 19 19 15 8 8 13 15 14 8 9 9 9 10 12 4 4 4 9 10 11 12 24 26 18
738
+ 13 13 11 11 13 10 16 14 13 12 4 4 4 17 11 7 7 8 7 8 8 8 20 20 26 16 14
739
+ 4 4 4 8 8 10 18 4 4 4 8 8 21 11 13 10 8 10 6 6 6 6 6 8 9 12 14 17 11
740
+ 14 14 8 8 8 7 9 7 8 8 12 9 9 8 8 7 10 10 12 10 9 8 16 9 8 8 8 7 6 6 8
741
+ 6 6 6 6 6 6 6 9 12 7 7 8 8 7 7 7 12 8 7 7
742
+
743
+
744
+ Sequence : 22ak70f10.s1t
745
+ Is_read
746
+ Padded
747
+ Staden_id 2
748
+ Clipping QUAL 35 508
749
+ Align_to_SCF 1 644 1 644
750
+ SCF_File 22ak70f10.s1tSCF
751
+ Primer Universal_primer
752
+ Strand Forward
753
+ Dye Dye_terminator
754
+ Template 22ak70f10
755
+ Clone bK216E10
756
+ Sequencing_vector "m13mp18"
757
+ Seq_vec SVEC 1 34 "M13mp1"
758
+ Seq_vec SVEC 584 644 "M13mp1"
759
+
760
+ DNA : 22ak92c4.s1t
761
+ nGGGCGCTGCCTGCAGGTCGACTCTAnAGGATCCCCATTATACGGGTTGAGTGTTTCTAA
762
+ TCTGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAA
763
+ TGTTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGGGTTGGGAATAT
764
+ TCAACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAATCTGAAACATT
765
+ TCTGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCTGTAGTACGTTCTTTATCAT
766
+ TGTTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAGCAAATTCGATTG
767
+ TATTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAGGATTATTGGAGAGAATAAA
768
+ ACAGTGAAGCTTTGGTGTTATGAGGGAATTTTAGATAGAAAAGTGCAGTTTTTCAGTTCA
769
+ TGCTCTTTCATTTC
770
+
771
+ BaseQuality : 22ak92c4.s1t
772
+ 4 4 6 4 6 8 11 14 18 26 32 34 36 34 23 25 10 23 9 23 23 34 29 23 22 4
773
+ 4 4 16 14 25 24 29 33 30 33 33 33 33 40 37 40 45 28 28 25 15 18 32 32
774
+ 17 14 28 13 13 38 38 40 40 37 37 37 37 38 45 45 45 38 33 32 33 33 33
775
+ 33 38 38 38 45 32 30 45 32 34 34 34 34 40 33 33 32 32 28 28 38 45 45
776
+ 49 45 45 45 45 40 37 41 45 45 40 30 30 33 33 33 33 40 40 45 45 45 45
777
+ 32 32 28 11 12 27 25 33 33 45 41 33 32 14 14 28 33 37 37 37 37 37 45
778
+ 45 37 37 37 37 37 37 37 37 28 33 32 21 14 32 20 24 32 21 21 32 36 41
779
+ 41 45 32 32 28 13 13 33 33 45 51 51 41 37 32 32 32 32 32 32 34 34 30
780
+ 30 31 32 12 12 27 31 30 35 35 35 33 39 41 28 28 28 28 28 28 41 41 41
781
+ 30 32 30 30 30 32 51 51 51 51 45 45 45 45 45 45 45 45 51 45 45 45 45
782
+ 33 33 33 33 33 33 51 51 51 51 51 41 41 45 45 45 39 39 39 39 39 39 41
783
+ 41 41 41 40 33 33 33 33 33 33 41 45 41 41 41 41 33 33 33 33 33 33 51
784
+ 51 45 34 22 22 20 28 30 17 15 15 45 30 33 33 33 33 33 33 33 33 37 37
785
+ 32 33 33 25 25 9 10 9 14 14 33 33 34 34 41 40 40 40 40 37 40 37 37 34
786
+ 33 33 29 24 22 32 32 45 45 40 27 27 14 32 32 45 38 34 45 19 21 10 21
787
+ 21 30 37 37 34 34 37 38 38 37 37 37 37 37 35 34 36 36 40 40 49 34 34
788
+ 34 34 37 37 38 37 33 30 30 30 30 30 40 40 34 34 34 27 27 31 31 33 35
789
+ 33 30 30 21 21 12 30 28 36 40 36 36 36 33 33 26 26 21 19 20 29 30 36
790
+ 34 34 33 31 28 28 32 35 32 35 26 30 25 26 26 25 21 19 12 11 19 21 23
791
+ 21 11 13 17 17 24 29 27 29 29 28 31 20 25 18 16 12 14 23 21 11 11 16
792
+ 14 10 21 23 33 33 34 31 31 22 22 14 21 11 16 18 31 40 40 36 36 24 27
793
+ 19 19 13 16 10 8
794
+
795
+ Sequence : 22ak92c4.s1t
796
+ Is_read
797
+ Padded
798
+ Staden_id 16
799
+ Clipping QUAL 37 490
800
+ Align_to_SCF 1 494 1 494
801
+ SCF_File 22ak92c4.s1tSCF
802
+ Primer Universal_primer
803
+ Strand Forward
804
+ Dye Dye_terminator
805
+ Template 22ak92c4
806
+ Clone bK216E10
807
+ Sequencing_vector "m13mp18"
808
+ Seq_vec SVEC 1 36 "M13mp1"
809
+
810
+ DNA : 22ap08f1.s1t
811
+ GAACTATCATnnnATATACGGGTTGAGTGTnTCTAATCTGAAAATCCAAAATTAGAAATG
812
+ CTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAATGTTCAAAGGAAGTGCTTATTGGA
813
+ GCATTATGGGTTGTTAgATTTTTGGGTtGGGAATATTCAACCATTAAGCTACACACAATG
814
+ CAAATATCCCACAAACAATCTGAAATCTGAAACACTTCTGGTCCAAGCAAGCATCCGCAA
815
+ CGGATACGCAACCTGCACACGTTCTTATCATCGTTAACTCTnATATATGTGGACAAATCT
816
+ GACGTGGTACACACACTCATGTATATCACAACATATTACATTTGAAGCTGCTACGAATAT
817
+ GGAAGAATnnnnCCTGAACTTGGTGTATGACGAACTTAGATAGACAGTGACTCTTCAGTT
818
+ CAGCTCnTCATTTnTnnTCCCTCGGTTTTnCTAAnCnCnnCnnAAAAnnnnATCCnGTGG
819
+ GCATAAAnnCnGGTCCCACCGCCCCCnCCCCACAAAACCACACACTACCGACCTCCCAA
820
+
821
+ BaseQuality : 22ap08f1.s1t
822
+ 7 9 10 13 14 16 21 15 14 4 4 4 4 4 11 11 20 26 28 28 30 30 30 25 25 20
823
+ 25 23 28 4 4 4 18 18 18 21 24 31 33 34 33 34 34 40 30 31 30 24 24 24
824
+ 31 27 30 31 30 23 32 32 37 36 38 34 33 24 18 15 10 8 8 18 25 32 33 34
825
+ 31 31 33 29 21 12 9 7 8 11 13 18 22 25 25 33 33 32 29 22 27 24 25 16 9
826
+ 9 19 21 20 14 16 10 12 12 11 11 18 23 21 19 19 10 10 10 20 9 9 13 18
827
+ 18 20 21 18 17 14 16 9 9 9 10 10 10 9 9 21 25 22 18 18 14 20 21 13 13
828
+ 21 17 26 25 23 20 15 10 12 16 17 15 10 11 8 8 8 8 6 4 4 4 8 10 8 8 6 6
829
+ 8 13 15 21 21 15 11 9 12 11 7 6 6 6 6 6 6 10 6 6 6 6 10 14 10 10 9 12
830
+ 9 11 10 14 9 13 10 10 12 9 9 15 17 20 16 17 21 25 10 8 10 10 7 7 10 9
831
+ 7 8 14 7 7 8 10 8 6 6 6 8 8 10 11 14 8 9 11 19 17 13 9 9 9 8 8 10 9 8
832
+ 8 8 9 8 11 11 10 8 8 8 8 6 6 8 10 8 7 6 4 8 4 4 4 7 7 11 10 12 9 12 10
833
+ 10 10 11 6 6 9 9 14 8 7 7 7 7 12 16 11 6 6 6 6 6 6 6 6 6 8 13 9 8 8 15
834
+ 12 10 9 8 8 8 8 7 9 12 8 6 6 8 4 6 6 7 11 13 13 7 8 9 7 7 12 12 10 9 4
835
+ 4 4 6 7 7 12 14 7 7 12 6 6 6 7 4 4 4 4 4 4 8 8 14 12 13 16 16 16 10 11
836
+ 16 14 12 7 6 6 4 4 4 8 7 7 7 12 7 7 8 11 4 4 4 4 4 6 6 6 6 6 6 6 6 6 4
837
+ 4 8 6 6 12 12 12 9 8 4 4 4 6 11 6 9 4 4 4 4 4 4 9 9 9 15 9 9 6 6 6 8 4
838
+ 4 4 9 9 4 4 4 4 4 4 4 4 4 4 4 9 9 4 4 4 4 4 4 11 12 4 4 4 8 8 15 9 6 8
839
+ 9 9 9 4 4 4 4 4 4 7 10 6 8 15 8 8 4 6 14 15 17 18 4 4 4 14 8 6 6 8 8 9
840
+ 13 9 9 9 7 7 7 7 8 8 6 6 8 6 6 6 8 6 6 8 8 8 8 4
841
+
842
+ Sequence : 22ap08f1.s1t
843
+ Is_read
844
+ Padded
845
+ Staden_id 17
846
+ Clipping QUAL 17 155
847
+ Align_to_SCF 1 539 1 539
848
+ SCF_File 22ap08f1.s1tSCF
849
+ Primer Universal_primer
850
+ Strand Forward
851
+ Dye Dye_terminator
852
+ Template 22ap08f1
853
+ Clone bK216E10
854
+ Sequencing_vector "m13mp18"
855
+ Tag DONE 137 137 "AUTO-EDIT: replaced T by g at 137 (terminated, isolated, strong)"
856
+ Tag DONE 148 148 "AUTO-EDIT: replaced A by t at 148 (terminated, isolated, strong)"
857
+
858
+ DNA : 22ai01c4.s1t
859
+ GGAACATGCTGCAGGTCGACTCTAGAGGATCCCCGCTCTACAGTCTGAAACTTTTTTGAG
860
+ CACCGACCTAATGTTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGG
861
+ GTTGGGAATATTCAACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAA
862
+ TCTGAAACATTTCTGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCTGTAGTACG
863
+ TTCTTTATCATTGTTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAGC
864
+ AAATTCGATTGTATTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAGGATTATTG
865
+ GAGAGAATAAAACAGTGAAGCTTTGGTGTTATGAGGGAATTTTAGATAGAAAAGTGCAGT
866
+ TTTTCAGTTCATGCTCTTTCATTTTTTACTCCCTCAGGTTAAAGCT-GAAGCTCAACAAA
867
+ GATATAGTGATCTCTGTGGGCATTTATAATCTGGTCCAGAAGGCTCTCAAGCCTCCTCCA
868
+ ATAAAGCTCTATCGGGAAACAAATGAAACAGTGAAAACCAAGAACCGGAACTTTAATACA
869
+ AGTACAGGCGGTTTGCTTTCTGCTAGCGATACCAAAAGTCTCAGGTAGGTAAAAATGCTn
870
+ nTGTGTTA
871
+
872
+ BaseQuality : 22ai01c4.s1t
873
+ 4 4 6 8 8 18 18 25 25 30 25 21 27 16 16 27 24 27 31 37 33 33 31 31 21
874
+ 21 23 25 37 34 37 45 45 45 45 40 40 40 33 33 30 30 30 30 38 45 45 49
875
+ 45 45 45 45 45 45 45 45 38 38 30 30 30 33 33 30 37 37 40 42 42 45 38
876
+ 45 35 25 27 33 33 33 33 37 37 37 40 37 37 37 37 42 42 42 40 37 40 45
877
+ 33 33 33 33 33 33 45 45 41 41 45 37 30 41 32 41 45 37 37 41 41 45 45
878
+ 41 32 38 37 30 30 30 41 41 51 51 51 51 33 33 35 35 35 29 33 33 33 33
879
+ 33 45 37 37 37 37 37 40 40 40 40 45 43 35 33 33 33 33 33 43 45 43 32
880
+ 33 33 28 28 29 51 51 45 51 51 51 51 51 51 51 51 51 51 51 51 51 51 42
881
+ 42 42 40 39 39 51 51 45 45 45 38 38 37 37 38 33 33 33 33 33 33 37 40
882
+ 39 39 42 33 33 33 33 33 33 37 37 41 41 41 41 33 33 33 33 33 33 51 45
883
+ 41 41 39 34 34 29 31 37 34 34 45 37 38 42 33 32 32 32 32 32 37 51 32
884
+ 32 32 32 32 21 24 12 25 25 33 33 33 33 40 40 40 37 37 41 45 45 45 35
885
+ 34 34 34 34 34 41 41 45 45 45 41 37 37 41 40 45 37 37 42 33 33 22 33
886
+ 33 33 37 37 37 37 37 37 37 37 37 36 33 33 33 33 33 33 40 40 45 34 34
887
+ 34 34 34 34 37 37 40 33 33 33 33 33 36 36 40 34 37 37 37 37 38 38 36
888
+ 33 33 33 31 31 20 29 27 34 34 34 40 40 34 34 38 38 38 34 34 40 37 40
889
+ 37 30 30 30 30 30 34 36 40 40 36 36 28 33 32 27 27 18 14 14 18 23 27
890
+ 34 33 29 29 24 29 29 27 27 28 28 25 25 33 22 24 23 31 28 33 34 31 25
891
+ 25 25 25 30 31 36 32 27 18 13 13 25 25 17 23 25 31 45 45 45 38 31 31
892
+ 24 22 20 27 35 34 31 33 33 33 33 36 36 36 38 20 13 10 8 12 11 18 20 23
893
+ 34 34 30 1 29 22 19 24 22 24 28 31 31 31 27 27 23 28 10 25 20 17 10 25
894
+ 25 35 35 38 38 36 36 27 27 17 17 11 13 16 19 18 18 18 22 15 11 11 15
895
+ 27 25 24 24 18 8 9 8 7 7 8 7 8 8 15 17 21 14 10 10 19 17 20 25 24 24
896
+ 21 16 15 21 21 26 33 33 26 23 19 23 23 28 21 20 15 10 8 7 8 6 8 11 7
897
+ 10 18 17 12 9 9 7 11 9 7 6 7 6 8 9 9 9 6 10 8 10 7 8 8 7 6 6 8 7 8 8
898
+ 14 23 24 19 14 14 7 7 7 7 8 8 6 6 7 6 6 6 6 6 9 21 13 12 13 15 15 10
899
+ 11 17 16 15 8 7 7 9 9 10 10 8 8 8 8 9 8 6 8 8 8 7 7 7 7 7 7 7 7 7 7 7
900
+ 10 8 7 7 8 11 4 4 4 4 9 6 6 6 6 6
901
+
902
+
903
+ Sequence : 22ai01c4.s1t
904
+ Is_read
905
+ Padded
906
+ Staden_id 6
907
+ Clipping QUAL 35 553
908
+ Align_to_SCF 1 466 1 466
909
+ Align_to_SCF 468 668 467 667
910
+ SCF_File 22ai01c4.s1tSCF
911
+ Primer Universal_primer
912
+ Strand Forward
913
+ Dye Dye_terminator
914
+ Template 22ai01c4
915
+ Clone bK216E10
916
+ Sequencing_vector "m13mp18"
917
+ Seq_vec SVEC 1 34 "M13mp18"
918
+
919
+ DNA : 22ak93c1.s1t
920
+ nGGTGAAGCTTGCATGCCTGCAGGTCGACTCTAAAGGATCCCCACAcCAAAGCTTCACTG
921
+ TTTTATTCTCTCCAATAATCCTAAgCAAGCTCTCAAAATATAAATATGCTTTtTAATAAT
922
+ ACAATCGAATTTGCTATACCACCTCAGAATCTGTACCACAATATATTAACTACTTAAAAC
923
+ AATGATAAAGAACGTACTACAGGTTGCGTATCCCTTTGCAAAATGCTTGCTTAgGACCAG
924
+ AAATGTtTCAGATTTCAGATTTTTTTTGGAATATTTGCATTTTATAGTACTTACTGGTTG
925
+ AATATTCCCAACCCAAAaATCTAACAACCCATAATGCTCCAATAAGCACTTCCTTTGAAC
926
+ ATTATGTCGGTGCTCAAAAAAGTTTCAGACTGTAAAGCATTTCCTAATTTTGGATTTCCA
927
+ GAATAGAAACACTCAACCCGTATAATAAAATGATAATCTGTATTAAGCACAGCACTATAT
928
+ CAAGCACCTCACAGCTATTCTCTCATTTAATCATCATAATAACCCTGTAAGTACTGTACA
929
+ AGTGGTCTGGTCCCTGCTTTTATTTCTCCAAGAACGACTCTCACACTATTGGTTCGCCTG
930
+ GAGTGCnTCGCGTGATCCCCGCTCATGGCACCTCCACTCCCGGGTCCAAGCAATTCTCTT
931
+ GCTCAACTTCCAAGTGGTGGGATTAGGGCCCTGCCATAACCCACTTATTTCTTTTATTTT
932
+ AATAAAACGTTTCCCCATTTTGGCAGTGGGTTTTACCCTGACCCGTATTGCCCTTCCGGC
933
+ CCACGATTTGGGGGAACCAGTTGGGCCCCCCTTTGGGCCTCTCCTGTTCCGAGC
934
+
935
+ BaseQuality : 22ak93c1.s1t
936
+ 4 4 4 4 8 8 12 20 25 31 33 33 30 30 30 31 31 35 35 34 27 23 12 11 25
937
+ 11 14 14 28 32 20 14 6 6 6 6 8 16 16 25 28 15 15 13 17 9 10 9 16 16 18
938
+ 18 18 25 27 30 26 20 21 19 10 10 14 23 25 30 30 37 37 37 34 31 28 30
939
+ 22 18 29 18 22 18 15 11 21 12 15 15 22 24 28 37 37 37 37 32 27 10 10
940
+ 13 18 20 25 25 23 23 24 32 33 40 40 38 32 18 18 18 27 27 28 37 37 21
941
+ 21 15 15 12 22 32 23 23 30 30 45 45 37 37 32 32 23 22 22 22 27 28 31
942
+ 26 23 12 12 18 32 38 37 37 37 37 34 34 30 30 30 30 30 30 34 34 37 37
943
+ 37 34 34 31 31 31 28 27 24 22 22 22 30 30 33 30 30 32 26 24 15 15 9 7
944
+ 7 14 10 10 9 14 11 12 10 11 6 7 9 8 17 24 26 28 26 28 28 35 34 34 30
945
+ 26 26 26 28 28 31 31 33 33 31 23 20 15 13 15 18 14 6 6 8 13 13 19 9 7
946
+ 7 16 10 10 15 10 8 8 12 10 9 9 18 13 14 17 11 7 7 13 21 23 28 21 20 19
947
+ 19 22 25 27 31 32 32 28 28 25 25 25 25 25 25 31 30 19 19 13 13 13 27
948
+ 28 33 33 33 33 33 36 33 31 24 21 14 15 18 24 27 31 25 28 30 30 28 23
949
+ 23 21 17 14 8 9 9 25 25 33 25 25 28 30 30 30 22 21 20 20 22 25 28 34
950
+ 28 21 16 15 10 14 17 25 25 12 12 12 12 12 20 10 14 16 21 17 13 11 9 12
951
+ 11 7 6 6 6 8 14 8 8 6 6 11 23 21 28 28 31 31 34 23 10 7 7 7 7 7 9 8 8
952
+ 14 16 20 20 16 10 7 7 7 14 7 9 14 10 9 14 9 19 22 27 25 13 13 18 14 17
953
+ 23 16 9 8 9 7 7 7 7 9 8 8 13 16 21 19 15 15 10 10 15 11 18 25 23 21 21
954
+ 21 32 25 28 25 28 17 20 10 7 7 11 6 7 6 6 7 9 8 6 6 6 6 6 8 7 7 9 13
955
+ 11 13 13 20 21 18 15 10 10 9 9 8 8 8 9 9 13 16 16 14 14 14 25 10 11 6
956
+ 6 6 6 7 10 14 9 13 20 8 7 7 8 11 10 13 12 12 13 16 14 15 15 13 13 10
957
+ 12 7 8 9 14 11 12 12 9 10 8 8 8 10 20 12 9 9 12 6 6 6 6 8 9 6 6 6 6 7
958
+ 6 6 8 16 18 18 9 6 6 6 13 7 7 7 8 9 9 7 7 7 7 7 6 6 6 6 6 6 6 6 7 8 8
959
+ 10 9 8 8 6 7 8 4 4 4 4 4 10 7 7 9 9 8 8 4 4 4 4 4 4 8 4 9 9 12 9 8 6 6
960
+ 6 9 11 7 7 7 7 7 9 11 11 9 6 6 6 10 8 8 7 10 10 14 25 16 10 7 7 7 7 10
961
+ 10 8 8 7 7 7 8 6 6 6 6 8 11 12 7 6 13 6 7 7 7 8 7 7 7 8 8 10 8 6 6 6 4
962
+ 4 8 8 10 13 22 24 24 20 14 8 6 6 6 6 6 10 15 15 10 9 6 6 10 8 7 7 9 9
963
+ 13 13 10 11 11 11 8 7 7 6 14 6 7 8 6 6 7 7 7 7 7 7 12 7 8 10 10 13 14
964
+ 9 8 8 8 11 9 7 7 7 8 8 11 10 12 11 11 10 10 12 12 17 13 9 8 9 6 6 6 6
965
+ 6 12 8 8 8 8 9 8 8 9 8 7 7 7 9 9 8 8 6 6 8 8 11 12 8 6 8 6 6 6 6 6 7 8
966
+ 9 11 12 12 6 6 8 7 8 8 13 7 7 7 6 8 8 8 8 8 8 6 6 8 4 6 4 4 4 4
967
+
968
+ Sequence : 22ak93c1.s1t
969
+ Is_read
970
+ Padded
971
+ Staden_id 10
972
+ Clipping QUAL 44 345
973
+ Align_to_SCF 1 834 1 834
974
+ SCF_File 22ak93c1.s1tSCF
975
+ Primer Universal_primer
976
+ Strand Forward
977
+ Dye Dye_terminator
978
+ Template 22ak93c1
979
+ Clone bK216E10
980
+ Sequencing_vector "m13mp18"
981
+ Seq_vec SVEC 1 43 "M13mp1"
982
+ Tag DONE 47 47 "AUTO-EDIT: replaced T by c at 47 (terminator, isolated, strong)"
983
+ Tag DONE 85 85 "AUTO-EDIT: replaced C by g at 85 (terminated, isolated, strong)"
984
+ Tag DONE 113 113 "AUTO-EDIT: replaced A by t at 113 (terminated, isolated, strong)"
985
+ Tag DONE 234 234 "AUTO-EDIT: replaced C by g at 234 (terminated, isolated, strong)"
986
+ Tag DONE 247 247 "AUTO-EDIT: replaced C by t at 247 (terminated, isolated, strong)"
987
+ Tag DONE 318 318 "AUTO-EDIT: replaced G by a at 318 (terminated, isolated, strong)"
988
+
989
+ DNA : 22ak65e10.s1t
990
+ TGAGGGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAAAATAAATAAATAA
991
+ ATAAATTAGCTGGGTGTGATGTCACATGCCTGTGATCCCAGCTACTTGGGAGGCTGAGAC
992
+ AGAAGGATCGCTTGAGTCCAGGAGGTCAAGGCTGTAGCGAGTCATGTTCGCCCCACTGCA
993
+ CTCCAGCCTGGGCGACACAATGAGACCCTGTCTCAAAAACAACAACAACAAAAGGCATCT
994
+ CTACCTACCTGAGACCTCTTGGTATCGCTAGGCAGAAGCAAACCGCCTGTACTTGTATTA
995
+ AAGGTCCGGGTCTTGGTTTTCACTGGTTCATTTGTTTCCCGATAGAGCTTTATTGGAGGA
996
+ gGCTTGAGAGCCTTCTGGACCAGATTATaAATGCCCACAGAGATCACTATATCTTTGTTG
997
+ AGCTTC-AGCTTTAACCTGAGGGAGTAAAAAATGAAAGAGCATGAACTGAAAAACTGCAC
998
+ TTTTCTATCTAAAATTCCCTCATAACACCAAAGCTTCACTGTTTTATTCTCTCCAATAAT
999
+ CCTAAGCAAGCTCTCAAAATATAAATATGCTTTTTAATAATACAATCC
1000
+
1001
+ BaseQuality : 22ak65e10.s1t
1002
+ 4 4 8 8 9 9 14 15 31 31 28 28 28 30 30 33 33 33 30 22 20 12 22 12 18
1003
+ 18 30 31 27 23 32 10 10 14 12 32 32 37 45 45 45 45 45 38 34 29 29 29
1004
+ 29 29 29 29 32 32 30 34 34 34 34 35 34 34 32 35 29 27 32 32 32 37 45
1005
+ 32 45 37 37 37 24 24 32 32 19 22 11 21 21 31 31 34 45 45 45 28 30 30
1006
+ 37 40 34 33 33 28 28 28 37 45 41 41 41 41 45 41 27 27 33 37 37 32 27
1007
+ 20 20 20 30 20 20 21 24 17 38 32 30 42 37 37 37 30 28 13 12 18 25 21
1008
+ 24 14 25 25 22 28 17 28 28 18 32 36 36 32 32 21 32 32 31 31 27 27 21
1009
+ 32 32 27 13 13 32 33 38 45 36 34 30 30 21 21 23 23 23 23 30 33 33 37
1010
+ 34 41 36 41 37 37 34 29 29 19 30 30 40 37 37 36 37 25 25 26 26 30 45
1011
+ 41 51 37 33 30 28 28 33 20 18 20 20 20 20 26 20 20 18 21 21 31 31 28
1012
+ 32 14 18 20 29 33 34 45 45 40 40 40 37 37 37 37 37 32 45 30 23 23 32
1013
+ 32 37 34 35 34 34 34 34 37 37 37 37 45 37 37 32 27 23 14 12 12 14 17
1014
+ 19 27 27 28 30 37 45 45 45 45 37 37 37 42 42 40 37 37 34 33 33 40 31
1015
+ 26 28 25 27 31 34 34 34 31 27 27 27 31 27 31 23 23 18 18 18 25 25 31
1016
+ 35 31 30 27 25 27 35 33 32 30 21 21 21 23 23 27 26 21 20 16 16 17 18
1017
+ 30 30 36 36 36 34 34 36 34 26 15 12 6 6 6 12 11 23 23 26 17 17 21 32
1018
+ 35 35 40 33 33 33 38 33 27 23 23 12 12 18 18 19 10 8 8 17 19 34 33 34
1019
+ 31 34 31 31 22 22 19 18 27 27 32 22 25 24 32 32 34 34 34 37 34 30 33
1020
+ 27 27 27 31 32 27 27 18 1 12 12 11 15 15 17 27 28 28 34 34 30 25 18 13
1021
+ 19 15 15 25 21 29 26 34 24 28 25 27 21 20 23 24 24 26 29 30 26 27 22
1022
+ 29 27 33 34 34 36 38 38 38 34 31 30 24 24 24 31 33 35 35 29 33 33 31
1023
+ 31 28 28 29 26 22 14 8 9 9 9 14 9 13 18 21 21 27 23 18 15 21 21 25 33
1024
+ 33 31 31 21 21 24 27 29 25 19 19 19 25 27 27 34 34 34 14 9 7 7 9 18 23
1025
+ 27 23 19 19 23 24 21 18 8 8 9 21 20 31 24 20 13 11 16 24 32 32 25 18
1026
+ 13 13 20 20 26 21 27 21 20 13 17 15 21 12 12 18 15 15 15 15 8 8 7 10 8
1027
+ 4
1028
+
1029
+
1030
+ Sequence : 22ak65e10.s1t
1031
+ Is_read
1032
+ Padded
1033
+ Staden_id 4
1034
+ Clipping QUAL 42 575
1035
+ Align_to_SCF 1 426 1 426
1036
+ Align_to_SCF 428 588 427 587
1037
+ SCF_File 22ak65e10.s1tSCF
1038
+ Primer Universal_primer
1039
+ Strand Forward
1040
+ Dye Dye_terminator
1041
+ Template 22ak65e10
1042
+ Clone bK216E10
1043
+ Sequencing_vector "m13mp18"
1044
+ Seq_vec SVEC 1 41 "M13mp"
1045
+ Tag DONE 361 361 "AUTO-EDIT: replaced C by g at 361 (all_stranded, compound, strong)"
1046
+ Tag DONE 389 389 "AUTO-EDIT: replaced C by a at 389 (terminated, isolated, strong)"
1047
+
1048
+ DNA : 22ak93c2.s1t
1049
+ CTGGGAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCACTCATTTTCTGCCTGG
1050
+ TGTTTATTCTCATCCAAATTCGTATACATGCTCGGTGTGAATGGTTACTCATTATAGTAT
1051
+ ACAATCGACTTTCTCAAACGACCACACATGCTGAATATCTATACATGTGCTAGTTGGACC
1052
+ TCTGATTATCAACGTGAGACTGGTGGCGTATCCCTTTGCAAAATGCTAACAAAAGACCAC
1053
+ AAACGTGTCACATTTCAGAATTGCTTGGAATATTCGCGTGTATAGTACTTACTGGTTCAA
1054
+ GGGTCCAAAACATACCTCTACACACCATCCTGATTCCCTGGGACACTTCCTTTGAACATC
1055
+ ACGTCGGTGCTCAAAAAAGTGTCAGACTGTAGAGCATATCTGGTTTCGGAATGTCGAGAT
1056
+ AGAAGGTCTCAAGCCGTATAAATAAACGAGAGTCTGTATCAAGCACTGCAGGAGAATCAT
1057
+ AACCTCACAGCTATGCTCTCATGGAAACATCTCAATACACCTCGTAGTACTGTACTAATA
1058
+ GTCATGTCGCTGCTTTTTGTTnCCGACAACCAATCTCACGGATTGGTTCGGCCGGTTTGC
1059
+ ATCCCTGATTCCGATCATTGAAGGTCGAACTCCCGGTCCAAGCAATCTCCTGCCCCGGCT
1060
+ CCAGATGACGGGATTCTTGCCTTTCAACACCCCGGCTAATTCATCCTCTCCCTCGACACC
1061
+ GATTTCACGAAATGGGGGTCGGCCCCAACCCGAACATTTGTTTACTGGGTAAAACGGTTC
1062
+ CCCAGTTACCCGTTGGCCCACTCCTGGGCCATTAATTGCCTC
1063
+
1064
+ BaseQuality : 22ak93c2.s1t
1065
+ 4 4 8 8 9 12 12 22 27 30 30 30 30 33 33 35 33 37 37 33 31 30 25 25 27
1066
+ 27 18 18 32 38 32 28 28 9 9 10 10 25 21 27 38 28 25 23 16 12 10 10 11
1067
+ 10 13 12 11 15 14 8 8 11 13 18 21 21 9 7 7 6 11 8 8 8 6 6 8 8 8 8 10 9
1068
+ 9 10 9 14 9 13 11 8 8 8 8 8 11 10 11 14 14 14 21 11 10 10 10 11 17 11
1069
+ 7 7 8 8 9 9 13 14 18 15 9 9 9 8 8 10 12 12 10 10 8 9 12 12 13 14 23 10
1070
+ 10 10 12 11 11 10 10 10 11 14 14 15 15 21 22 10 10 10 10 28 10 9 9 9
1071
+ 10 12 15 18 15 15 12 12 12 12 25 21 24 27 18 13 13 13 14 12 14 12 10
1072
+ 10 10 14 18 14 9 9 11 12 12 10 10 10 10 9 14 14 8 8 8 8 6 6 9 6 6 6 12
1073
+ 18 34 36 38 38 38 38 36 36 33 33 23 23 13 13 15 23 28 20 9 6 6 4 6 10
1074
+ 8 7 7 9 10 9 13 13 10 10 9 8 8 6 6 8 8 11 12 10 9 9 9 6 4 6 6 8 6 7 11
1075
+ 10 9 7 8 17 18 21 19 17 11 8 8 9 11 11 15 12 8 8 8 9 9 15 15 10 10 9
1076
+ 10 7 7 8 12 10 10 9 9 9 9 10 11 10 8 8 8 7 12 7 7 7 8 9 8 9 10 10 9 10
1077
+ 8 6 6 6 6 7 6 9 10 10 10 7 6 6 6 8 6 6 9 10 13 12 12 9 12 15 14 14 8 8
1078
+ 8 9 10 10 9 9 9 9 8 6 6 10 8 13 6 6 6 11 16 18 12 11 8 10 12 8 7 9 7 7
1079
+ 7 7 9 9 7 7 10 10 12 10 20 9 7 7 7 8 8 8 9 7 7 7 11 9 13 19 15 12 7 8
1080
+ 7 6 6 6 6 6 6 6 10 13 8 6 6 8 6 8 10 10 12 7 7 7 7 7 7 8 10 9 9 8 7 6
1081
+ 6 8 6 6 8 9 9 10 7 7 8 8 8 8 7 7 8 7 7 7 7 7 9 8 8 15 8 8 6 6 6 6 6 6
1082
+ 7 10 9 9 8 7 8 8 10 7 7 8 8 11 11 11 8 6 6 6 6 6 12 6 6 6 6 6 7 11 12
1083
+ 9 7 10 11 8 8 7 8 11 8 7 7 7 6 8 6 6 8 6 8 7 7 8 7 7 7 8 9 7 7 7 7 7 7
1084
+ 9 7 7 7 6 13 4 7 7 7 12 23 21 14 12 9 8 9 8 4 4 4 6 6 6 7 6 8 4 4 4 6
1085
+ 6 6 6 6 6 8 6 7 10 9 9 9 8 6 4 4 4 6 8 6 6 6 6 8 6 6 8 8 8 8 8 8 6 6 8
1086
+ 11 10 7 7 7 7 9 9 9 8 7 11 8 7 7 8 9 8 8 7 7 7 7 7 7 7 7 8 7 7 7 7 8 7
1087
+ 7 8 7 7 7 7 8 7 7 7 7 7 7 6 6 7 7 6 6 6 8 9 8 7 7 7 7 8 8 12 9 7 7 7 7
1088
+ 6 7 8 7 7 7 8 8 6 6 6 6 6 6 7 7 9 12 10 14 8 9 8 6 6 6 6 6 6 9 10 10 9
1089
+ 6 6 6 6 8 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 7 7 7 6 6 6 4 6 6 6 6 8 6
1090
+ 6 6 6 6 8 8 10 7 4 7 4 4 4 6 6 6 6 6 6 6 6 6 6 6 7 7 9 9 8 8 7 9 9 7 7
1091
+ 7 8 6 6 6 6 6 6 6 6 6 6 8 8 8 8 8 8 8 7 7 6 6 6 6 7 8 9 9 9 9 7 7 7 7
1092
+ 7 7 7 6 6 6 6
1093
+
1094
+ Sequence : 22ak93c2.s1t
1095
+ Is_read
1096
+ Padded
1097
+ Staden_id 13
1098
+ Clipping QUAL 44 45
1099
+ Align_to_SCF 1 822 1 822
1100
+ SCF_File 22ak93c2.s1tSCF
1101
+ Primer Universal_primer
1102
+ Strand Forward
1103
+ Dye Dye_terminator
1104
+ Template 22ak93c2
1105
+ Clone bK216E10
1106
+ Sequencing_vector "m13mp18"
1107
+ Seq_vec SVEC 1 43 "M13mp1"
1108
+
1109
+ DNA : 22am84d9.s1
1110
+ CAACCCGCACACCTGCACGTCGACTCTAGAGGATCCCCATAAATAAATAAATtAgCTGGG
1111
+ TGTGATGTCACATGCCTGTgATCCCAGCTACTTGGGAgGCTGAGACAGAAGGATCGCTTG
1112
+ AGTCCAGGAGGTCAAGGCTGTAGCGAGTCATGTTCGCCCCACTGCACTCCAGCCTGGGCG
1113
+ ACACAATGAGACCCTGTCTCAAAAACAACAACAACAAAAGGCATCTCTACCTACCTGAGA
1114
+ CCTCTTGGTATCGCTAGGCAGAAGCAAACCGCCTGTACTTGTATTAAAGGTCCGGGTCTT
1115
+ GGTTTTCACTGGTTCATTTGTTTCCCGATAGAGCTTTATTGGAGGAGGCTTGAGAGCCTT
1116
+ CTGGACCAGATTATAAATGCCCACAGAGATCACTATATCTTTGTTGAGCTTCAAGCTTTA
1117
+ ACCTGAGGGCAGTAAAAAATGGAAGAGCATGAACTGGCAAAATGCACTTTTCTATCTAAA
1118
+ ATTCC
1119
+
1120
+ BaseQuality : 22am84d9.s1
1121
+ 4 9 9 9 9 10 10 9 9 9 9 10 10 10 8 8 6 6 10 8 11 12 15 14 13 15 11 11
1122
+ 11 12 14 10 10 10 17 23 31 31 21 20 13 21 11 10 10 16 14 14 14 23 11
1123
+ 11 10 10 10 11 11 12 32 18 23 23 32 32 34 37 34 37 37 38 27 18 18 17
1124
+ 24 30 32 32 10 10 11 23 23 38 37 37 37 37 37 37 37 37 37 37 32 32 22
1125
+ 22 22 32 28 40 37 37 37 45 32 32 28 22 22 12 16 16 32 32 37 37 37 37
1126
+ 28 28 28 32 32 30 30 27 29 29 34 37 37 37 37 37 37 37 37 37 28 22 20
1127
+ 30 30 29 37 34 37 37 37 37 37 37 37 37 37 37 37 45 45 37 37 37 37 45
1128
+ 37 37 34 34 34 33 31 33 34 37 27 27 13 13 13 21 32 34 36 31 31 33 19
1129
+ 17 14 9 9 13 24 27 27 33 33 33 33 33 33 33 33 33 36 36 37 37 37 37 37
1130
+ 45 45 36 34 34 28 31 31 34 34 37 38 34 38 38 33 30 30 30 30 30 33 34
1131
+ 37 37 37 34 34 34 34 34 35 35 33 33 33 33 33 33 34 30 30 30 30 30 30
1132
+ 33 33 30 30 30 34 34 34 34 31 31 31 36 36 34 31 17 13 8 8 15 18 27 31
1133
+ 28 28 28 28 28 30 31 35 35 34 34 34 34 33 33 33 34 34 38 38 38 37 32
1134
+ 28 22 19 19 25 27 37 30 30 30 28 28 28 38 37 34 34 37 31 31 25 24 28
1135
+ 30 30 28 28 28 28 28 28 34 34 34 33 33 31 31 27 24 18 15 18 18 23 23
1136
+ 28 27 22 18 11 11 16 21 24 33 34 23 23 8 8 9 12 14 17 20 20 20 25 25
1137
+ 27 27 23 16 11 15 14 9 9 19 17 18 22 18 11 11 11 12 17 18 31 31 31 31
1138
+ 27 27 33 29 22 18 17 17 20 20 17 13 11 11 11 6 8 14 17 22 22 25 25 20
1139
+ 20 20 17 14 4 4 4 14 15 21 22 18 11 12 7 7 7 7 7 12 21 18 15 13 9 9 15
1140
+ 12 8 6 6 6 6 9 6 6 6 12 14 13 13 17 21 21 24 21 22 18 14 18 16 14 14
1141
+ 10 6 7 6 13 8 8 7
1142
+
1143
+
1144
+ Sequence : 22am84d9.s1
1145
+ Is_read
1146
+ Padded
1147
+ Staden_id 7
1148
+ Clipping QUAL 39 427
1149
+ Align_to_SCF 1 485 1 485
1150
+ SCF_File 22am84d9.s1SCF
1151
+ Primer Universal_primer
1152
+ Strand Forward
1153
+ Dye Dye_primer
1154
+ Template 22am84d9
1155
+ Clone bK216E10
1156
+ Sequencing_vector "m13mp18"
1157
+ Seq_vec SVEC 1 38 "M13mp18"
1158
+ Tag ALUS 41 210
1159
+ Tag DONE 53 53 "AUTO-EDIT: replaced C by t at 53 (all_stranded, isolated, strong)"
1160
+ Tag DONE 55 55 "AUTO-EDIT: replaced C by g at 55 (all_stranded, isolated, strong)"
1161
+ Tag DONE 80 80 "AUTO-EDIT: replaced C by g at 80 (all_stranded, isolated, strong)"
1162
+ Tag DONE 98 98 "AUTO-EDIT: replaced C by g at 98 (all_stranded, isolated, strong)"
1163
+ Tag AMBG 338 360 "AUTOEDIT: Check this edit cluster!"
1164
+ Tag CONF 413 413 "AUTOEDIT: possible compression"
1165
+
1166
+ DNA : 22ak66f10.s1lt
1167
+ CCCGCGGGGGAGGAGGGAnGGGGGGAGAGGAGnAnAAnnnnnnnnnnnnnAGCCCCAAnn
1168
+ nAnnCCCTCTTnnnnnnnnAGACGGGGGGnGCCCTGGGnGnGCnTnnnnnnnnnnTTGGT
1169
+ TGAGGTGnCGAGCAAATCTTGCATGCCTGtcCGTCTACTCtAGAGGATCCCACAAATGAA
1170
+ CCAGTGAAAACCAAGACCCGGACCTTTAATACAAGTACAGGCGGTTTGCTTCTGCCTAGC
1171
+ GATACCAAGAGGTCTCAGGTAGGTAGAGATGCCTTTTGTTGTTGTTGTTTTTGAGACAGG
1172
+ GTCTCATTGTGTCGCCCAGGCTGGAGTGCAGTGGGGCGAACATGACTCGCTACAGCCTTG
1173
+ ACCTCCTGGACTCAAGCGATCCTTCTGTCTCAGCCTCCCAAGTAGCTGGGATCACAGGCA
1174
+ TGTGACATCACACCCAGCTAATTTATTTATTTATTTATTTTTTAAGAGACTGGATCGACT
1175
+ GGGCACAGTGGCTCATGCCTGTAATCCCAnCACTTTGGGAGGCCGAGGCAGGTGGATTAC
1176
+ CGAGGTCAGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCCCATCTCTACTAAAAA
1177
+ TACAAAATGAGCTGGGCATGGTGGTGCATGCCTGTAATCCCAGCTACTCCGGAGGCTGAG
1178
+ GCAnGAGAATCCCTTGAACCCGGGAGGCAAAAGTTGCGGTGAGCCGAAATCTCGCCATTG
1179
+ CGCTCCAGCCTGGGCAACAAAAGCGTAACTCCGTCTCCnnnnnnnnAAAAAAAGAGACGG
1180
+ TGTTTCGCTATGTTGCCTGGGCTGGGTGTCAAACTCCTGGGTCTTAAAGTAATCCCCCCC
1181
+ TGCTTTGGGTTCCCCCAAATTGCTGGGGGTGGCTGGGATTCCCGAACTTTGGGGCCACTG
1182
+ TTGCCCCAGCCGTTTTTTTGGTTTCGTTTTGGTTTTGGTTTTGTTTTAATAAAAAAATAA
1183
+ AGGGTCTCACCTATGTT
1184
+
1185
+ BaseQuality : 22ak66f10.s1lt
1186
+ 6 6 4 6 8 8 11 8 8 6 6 11 4 4 4 12 6 4 4 4 8 7 6 6 6 6 6 6 6 8 4 4 4 4
1187
+ 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 7 7 6 6 6 8 4 4 4 4 4 4 4 4 6 4 6 6
1188
+ 8 4 4 4 4 4 4 4 4 4 4 4 4 6 6 6 6 6 6 4 4 4 6 6 4 4 8 12 4 4 4 4 4 4 4
1189
+ 4 4 4 4 4 4 4 4 4 4 4 4 6 6 4 4 4 4 4 4 8 6 4 4 4 4 4 4 15 17 8 4 4 6
1190
+ 9 21 21 30 28 30 33 33 21 18 8 8 8 10 15 23 21 26 26 19 14 7 7 8 8 10
1191
+ 18 21 18 10 9 9 18 14 24 24 29 32 34 37 34 36 32 32 30 30 30 34 38 44
1192
+ 45 37 37 34 27 27 15 15 23 32 40 40 37 37 34 45 45 45 45 45 42 25 25
1193
+ 25 26 26 20 23 28 30 33 33 33 34 34 34 34 37 45 45 45 45 49 49 49 49
1194
+ 49 45 45 43 38 38 37 37 40 37 37 34 27 27 12 10 18 14 32 27 27 27 29
1195
+ 29 12 18 10 15 10 21 10 14 10 19 19 41 45 45 45 45 45 40 40 34 37 37
1196
+ 45 45 45 45 45 45 45 45 45 40 51 37 37 25 22 18 27 22 25 25 29 29 28
1197
+ 33 33 40 37 37 37 37 37 37 34 37 37 42 35 32 30 30 37 38 45 45 30 30
1198
+ 30 33 31 33 32 37 45 45 45 32 30 30 37 33 37 37 40 37 37 37 37 37 37
1199
+ 37 37 37 37 34 34 34 37 45 45 45 45 45 45 45 45 49 45 45 45 45 33 33
1200
+ 29 29 33 33 33 33 33 37 40 45 45 45 45 45 45 45 35 35 35 35 35 40 38
1201
+ 45 45 45 37 37 32 22 14 14 14 32 45 45 45 38 37 31 28 27 30 28 27 22
1202
+ 27 27 31 31 34 31 28 28 27 31 33 33 33 33 33 33 32 28 28 30 34 37 37
1203
+ 35 35 35 37 37 33 33 33 33 33 33 31 31 30 30 33 31 31 36 33 33 33 34
1204
+ 25 25 10 10 10 19 19 34 34 34 34 31 31 34 34 36 38 30 30 26 23 30 31
1205
+ 34 32 45 37 37 37 37 33 33 40 40 40 36 33 31 31 31 33 33 23 22 4 4 4
1206
+ 18 23 32 34 36 36 32 28 21 21 21 29 20 28 25 24 18 25 21 27 27 32 32
1207
+ 34 31 31 34 37 37 37 37 32 32 10 21 10 25 28 32 29 34 28 31 27 27 28
1208
+ 22 22 27 29 32 32 37 37 37 37 37 37 37 37 35 34 34 34 31 27 27 19 19
1209
+ 30 34 37 38 34 34 34 34 34 40 38 38 38 38 37 37 37 35 35 32 28 28 28
1210
+ 30 30 30 33 30 30 22 22 22 32 32 35 35 34 34 35 35 34 37 37 38 30 24
1211
+ 24 24 24 24 31 38 38 32 27 27 27 35 25 18 12 9 9 11 21 23 18 12 6 6 8
1212
+ 8 8 9 10 17 10 14 8 9 8 7 4 4 4 16 14 14 12 15 8 8 11 15 14 25 25 25
1213
+ 28 33 33 33 21 29 7 7 7 8 8 7 8 9 9 8 11 24 22 31 25 23 23 15 13 10 7
1214
+ 9 9 8 8 12 7 7 7 16 11 18 14 25 25 22 19 16 17 14 8 9 10 13 15 27 28
1215
+ 31 23 22 14 14 13 15 15 23 21 8 8 6 6 6 6 9 18 20 28 22 22 20 17 14 9
1216
+ 4 4 4 4 4 4 4 4 4 4 16 10 13 20 23 23 12 9 9 9 17 10 9 7 8 6 6 8 4 4 7
1217
+ 12 13 14 7 8 8 8 8 8 18 21 16 9 10 9 7 9 7 9 8 10 7 7 7 12 9 9 7 7 8 8
1218
+ 6 6 8 8 8 6 8 6 6 6 6 7 6 11 6 8 6 6 6 6 6 8 9 9 8 9 12 8 8 8 7 8 8 7
1219
+ 8 6 6 6 9 8 6 6 6 10 13 11 7 9 7 7 7 7 7 8 9 7 7 9 9 7 4 4 4 7 11 7 7
1220
+ 9 8 7 6 6 6 6 6 8 8 10 9 8 8 6 6 6 6 7 6 6 4 4 4 4 8 6 8 13 16 20 8 8
1221
+ 7 7 7 7 7 7 7 8 12 8 8 6 6 6 6 6 6 6 6 6 6 9 8 8 6 6 6 8 6 9 14 9 9 8
1222
+ 9 7 6 6 8 6 6 8 7 6 6 8 6 6 7 7 7 7 7 7 8 8 4 4
1223
+
1224
+
1225
+ Sequence : 22ak66f10.s1lt
1226
+ Is_read
1227
+ Padded
1228
+ Staden_id 9
1229
+ Clipping QUAL 140 640
1230
+ Align_to_SCF 1 977 1 977
1231
+ SCF_File 22ak66f10.s1ltSCF
1232
+ Primer Universal_primer
1233
+ Strand Forward
1234
+ Dye Dye_primer
1235
+ Template 22ak66f10
1236
+ Clone bK216E10
1237
+ Sequencing_vector "m13mp18"
1238
+ Seq_vec SVEC 1 3 "M13mp18"
1239
+ Tag DONE 150 150 "AUTO-EDIT: replaced C by t at 150 (all_stranded, compound, strong)"
1240
+ Tag DONE 151 151 "AUTO-EDIT: replaced G by c at 151 (all_stranded, compound, strong)"
1241
+ Tag DONE 161 161 "AUTO-EDIT: replaced A by t at 161 (all_stranded, compound, strong)"
1242
+ Tag ALUS 283 462
1243
+ Tag ALUS 477 767
1244
+
1245
+ DNA : 22ak70h4.r1t
1246
+ TGCCGCCTTAGATTACAGCTCTACACGGTACCTCTATCGGGAAACAAATGAACCAgTGAA
1247
+ AACCAAGACCCGGACCTTTAATACAAGTACAGGCGGTTTGCTTCTGCCTAGCGATACCAA
1248
+ GAGGTCTCAGGTAGGTAGAGATGCCTTTTGTTGTTGTTGTTTTTGAGACAGGGTCTCATT
1249
+ GTGTCGCCCAgGCTGGAGTGCAGTGGGGCGAACATGACTCGCTACAGCCTTGACCTCCTG
1250
+ GACTCAAGCGATCCTTCTGTCTCAGCCTCCCAAGTAGCTGGGATCACAGGCATGTGACAT
1251
+ CACACCCAGCTAATTTATTTATTTATTTATTTTTTAAGAGACTGGATCGACTGGGCACAG
1252
+ TGGCTCATGCCTGTAATCCCACCACTTTGGGAGGCCGAGGCATGTGGATTACCGAGGTTC
1253
+ GGAGTTCAAGACCAGCCTGACCCTTCTGGAGAAACCCCATCTCCACTTTAAATACCAAAT
1254
+ GAAGCTGGGCCTGGTGGTGCCTGCCTGTAATCCCCCCTTCTCAGGATGCTGAAGCAGGAC
1255
+ ATTCCTTGAACCCCGGAAGCACATGTTGCCGTGACCCAATATCCCCCCTTGTCCCTCACC
1256
+ CCGGGCCACCAAACCTTATTCCCTCCCAAATAAAAAAAAAAAAAAACGGGTTCCCTTTTT
1257
+ TGCCGGGGGGGGTTTCTACCCC
1258
+
1259
+ BaseQuality : 22ak70h4.r1t
1260
+ 4 4 4 4 4 8 6 6 8 8 12 6 7 11 13 12 6 6 6 6 6 6 9 10 10 10 21 21 26 31
1261
+ 27 27 13 14 11 11 11 12 19 20 27 18 18 23 23 16 10 10 14 28 18 18 11
1262
+ 11 9 9 9 10 10 10 21 21 27 23 18 10 9 9 14 18 32 31 37 34 28 28 22 19
1263
+ 19 27 24 21 18 17 9 9 12 19 24 28 30 30 30 33 33 30 30 37 40 40 45 37
1264
+ 41 41 44 44 45 32 42 37 32 32 32 32 37 31 31 33 21 21 11 10 22 18 32
1265
+ 24 27 27 27 27 11 14 10 23 12 23 9 10 10 18 11 25 25 34 45 37 37 34 34
1266
+ 34 34 34 34 34 33 33 33 34 34 34 34 33 34 28 31 25 21 23 21 21 13 13
1267
+ 15 15 17 23 21 26 26 31 28 32 23 23 12 22 25 21 19 9 8 8 17 21 23 23
1268
+ 20 18 17 27 23 21 27 30 38 38 28 28 17 17 13 18 19 27 25 25 28 28 28
1269
+ 28 25 31 26 26 18 15 13 9 9 15 19 23 25 31 36 36 36 36 36 36 36 36 24
1270
+ 19 12 13 18 24 31 31 29 29 31 29 29 33 36 40 44 36 33 34 34 37 36 36
1271
+ 45 45 34 30 28 28 28 22 32 24 22 22 32 32 36 37 27 27 14 25 25 29 23
1272
+ 22 19 17 19 19 24 25 27 24 18 18 16 16 16 24 22 30 30 28 28 30 27 27
1273
+ 23 20 22 25 25 25 21 22 21 16 14 19 17 17 19 20 17 11 11 11 11 12 24
1274
+ 24 22 20 34 17 17 23 23 31 34 27 27 17 13 15 27 27 34 31 31 24 22 19
1275
+ 16 16 15 32 23 27 18 9 9 9 13 9 14 20 22 21 21 19 17 14 8 6 6 8 8 9 11
1276
+ 14 8 7 11 15 14 9 9 9 12 8 10 6 8 8 6 4 4 4 14 15 23 21 13 13 11 13 11
1277
+ 11 6 8 8 6 6 6 7 6 6 6 6 7 6 6 11 15 17 27 20 17 17 13 21 13 14 4 4 8
1278
+ 6 6 6 6 8 6 8 11 7 8 8 10 10 19 24 16 16 9 9 12 7 7 8 8 8 8 6 6 11 15
1279
+ 11 10 9 9 11 14 15 11 8 7 7 7 7 12 9 10 10 8 8 14 10 10 18 21 21 18 16
1280
+ 10 9 11 9 9 14 10 10 8 8 9 8 8 12 14 10 10 11 11 10 9 8 7 7 7 7 4 4 4
1281
+ 12 10 14 10 8 14 6 6 6 7 6 6 6 6 6 10 8 9 7 7 7 9 14 15 10 10 10 10 9
1282
+ 8 13 8 4 4 4 4 4 6 6 12 6 6 10 8 8 8 6 9 12 7 6 6 6 8 8 9 7 7 7 8 9 8
1283
+ 8 6 6 6 6 6 4 6 6 6 6 7 8 8 6 6 8 8 8 8 9 9 8 10 11 9 8 8 8 8 6 6 11 9
1284
+ 9 10 9 12 8 6 6 6 10 12 10 10 8 8 8 7 10 13 8 13 19 18 13 6 6 6 6 8 6
1285
+ 7 12 7 7 10 10 7 7 7 7 7 7 9 13 6 10 7 7 12 11 7 6 6 6 6 8 8 8 4
1286
+
1287
+
1288
+ Sequence : 22ak70h4.r1t
1289
+ Is_read
1290
+ Padded
1291
+ Staden_id 15
1292
+ Clipping QUAL 28 377
1293
+ Align_to_SCF 1 682 1 682
1294
+ SCF_File 22ak70h4.r1tSCF
1295
+ Primer Universal_primer
1296
+ Strand Reverse
1297
+ Dye Dye_terminator
1298
+ Template 22ak70h4
1299
+ Clone bK216E10
1300
+ Sequencing_vector "m13mp18"
1301
+ Seq_vec SVEC 1 27 "M13mp18"
1302
+ Tag DONE 56 56 "AUTO-EDIT: replaced C by g at 56 (all_stranded, isolated, strong)"
1303
+ Tag ALUS 155 334
1304
+ Tag DONE 191 191 "AUTO-EDIT: replaced T by g at 191 (all_stranded, isolated, strong)"
1305
+ Tag ALUS 349 579
1306
+
1307
+ Sequence : Contig1
1308
+ Is_contig
1309
+ Padded
1310
+ Assembled_from 22ak93c2.r1t 1 293 39 331
1311
+ Assembled_from 22ak93c1.r1ta 1 305 31 335
1312
+ Assembled_from 22am91h5.r1t 110 439 32 361
1313
+ Assembled_from 22aq40a8.s1 221 570 39 388
1314
+ Assembled_from 22an02b10.s1t 252 666 32 446
1315
+ Assembled_from 22ak67d6.r1t 260 522 30 292
1316
+ Assembled_from 22ak64b1.s1t 331 707 40 416
1317
+ Assembled_from 22ak81h10.s1t 331 713 36 418
1318
+ Assembled_from 22ak66c6.s1t 337 848 37 548
1319
+ Assembled_from 22ak93c1.s1lt 1024 482 56 598
1320
+ Assembled_from 22ak64e2.s1t 866 487 41 420
1321
+ Assembled_from 22ak70f10.s1t 554 1027 35 508
1322
+ Assembled_from 22ak92c4.s1t 635 1088 37 490
1323
+ Assembled_from 22ap08f1.s1t 639 777 17 155
1324
+ Assembled_from 22ai01c4.s1t 682 1200 35 553
1325
+ Assembled_from 22ak93c1.s1t 1036 735 44 345
1326
+ Assembled_from 22ak65e10.s1t 1499 966 42 575
1327
+ Assembled_from 22ak93c2.s1t 1036 1035 44 45
1328
+ Assembled_from 22am84d9.s1 1488 1100 39 427
1329
+ Assembled_from 22ak66f10.s1lt 1174 1674 140 640
1330
+ Assembled_from 22ak70h4.r1t 1190 1539 28 377
1331
+
1332
+ DNA : Contig1
1333
+ TTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTATAnACTGTGCGTGCGC
1334
+ CACCATGCCTGGCTAATTTTTGTATTTTTAGTAGGGATGGGGTTTCACCATGTTGGCCAA
1335
+ GCTGGTCTCGAGCTCCTGACCTAGGATTACAGGCCTAAGCCACCGCACCCGGCATGATGG
1336
+ GTCTTTATTCTTCAAAGCAGGAGGAAGGGATCCTAGAAAAACAGAGACAAGGCCAAACAT
1337
+ GGTAGCTCACACCTGTAATnnnnnCACTTTGGGAGGCCAGTGCGGGTGAATCACGAnGTC
1338
+ AGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAnCACCGTCTCTACTAAAATAAAAAGA
1339
+ AATTAGCTGGGTGTCGTGGCAGGTGCnTGTAATCCCAGCCACTTGGGAAGCTGAGGCAGG
1340
+ AGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCGACTGCACTC
1341
+ CAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGACAAGACnACT
1342
+ AGTACAGTACTTACAGGGTTATTATGATGATTAAATGAGAGAATAGCTGTGAGGTGATTG
1343
+ ATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATACGGGTTGAGTGTnTCTAATC
1344
+ TGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAATG
1345
+ TTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGGGTTGGGAATATTC
1346
+ AACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAATCTGAAACATTTC
1347
+ TGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCTGTAGTACGTTCTTTATCATTG
1348
+ TTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAGCAAATTCGATTGTA
1349
+ TTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAGGATTATTGGAGAGAATAAAAC
1350
+ AGTGAAGCTTTGGTGTTATGAGGGAATTTTAGATAGAAAAGTGCAGTTTTTCAGTTCATG
1351
+ CTCTTTCATTTTTTACTCCCTCAGGTTAAAGCTnGAAGCTCAACAAAGATATAGTGATCT
1352
+ CTGTGGGCATTTATAATCTGGTCCAGAAGGCTCTnnAnnCnnnTCCnnnnnnnCTnnAnn
1353
+ nnnnnACAAATGAACCAGTGAAAACCAAGACCCGGACCTTTAATACAAGTACAGGCGGTT
1354
+ TGCTTCTGCCTAGCGATACCAAGAGGTCTCAGGTAGGTAGAGATGCCTTTTGTTGTTGTT
1355
+ GTTTTTGAGACAGGGTCTCATTGTGTCGCCCAGGCTGGAGTGCAGTGGGGCGAACATGAC
1356
+ TCGCTACAGCCTTGACCTCCTGGACTCAAGCGATCCTTCTGTCTCAGCCTCCCAAGTAGC
1357
+ TGGGATCACAGGCATGTGACATCACACCCAGCTAATTTATTTATTTATTTATTTTTTAAG
1358
+ AGACTGGATCGACTGGGCACAGTGGCTCATGCCTGTAATCCCAnCACTTTGGGAGGCCGA
1359
+ GGCAGGTGGATTACCGAGGTCAGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCCC
1360
+ ATCTCTACTAAAAATACAAAATGAGCTGGGCATGGTGGTGCATGCCTGTAATCC
1361
+
1362
+
1363
+
1364
+ DNA : 22ak93c2.r1t
1365
+ GTCGCnCATAAGATTACGAGATCTCGAGCTCGGTACCCTTCAAGCGATTCTCCTGCCTCA
1366
+ GCCTCCCGAGTAGCTGGGATTATAGACTGTGCGTGCGCCACCATGCCTGGCTAATTTTTG
1367
+ TATTTTTAGTAGGGATGGGGTTTCACCATGTTGGCCAAGCTGGTCTCGAGCTCCTGACCT
1368
+ AGGATTACAGGCCTAAGCCACCGCACCCGGCATGATGGGTCTTTATTCTTCAAAGCAGGA
1369
+ GGAAGGGATCCTAgAAAAACAGAGACAAGGCCAAACATGGTAgCTCACACCTGTAATCCC
1370
+ AnCACTTTGGGAGGCCagTGCGGGTGAATCACGAAGTCAGGAGTTCAAGACCACCCTGGC
1371
+ CAACATGGTGTAACACCTGCTCTCCTAAAATTAAACAAAATTTCATGGTTTGCGTGGGCC
1372
+ GTCTTGTCTCATCACTTCACTCCTGAGGGCCGGCGCCGGAAAGATATCTTGATCTGCGGC
1373
+ GCTCCGACCGTTTTCTTTAAACCTTACAACTCCCGACCTCCTCGCCTATCCTCCCTAAAT
1374
+ CCTCGCCAGGCTCGCCTGCTTCAGCCACTCTTTCCTTCGCACCCTCCCCTCTCTTCAATA
1375
+ TACTTCACCCGCCCATCCTTCACGCCGGCACGTATCCAATCTCTTCTTATCTTTCCGTAT
1376
+ CCAAnTCCCTTCTCCCTCTGCCGCGACCTTCGCCATCCCTCTGCGCGTCCTCTTCC
1377
+
1378
+ BaseQuality : 22ak93c2.r1t
1379
+ 4 4 8 4 4 4 4 4 4 4 4 4 6 8 17 21 14 7 6 6 6 7 7 6 8 14 16 21 15 20 20
1380
+ 24 26 21 18 18 14 14 19 23 10 8 8 15 20 16 29 26 34 29 39 29 31 29 31
1381
+ 34 32 27 27 25 19 19 24 31 33 36 34 34 34 26 27 22 32 32 36 28 28 15
1382
+ 15 15 28 28 34 30 12 12 22 27 31 31 31 31 31 23 24 27 21 24 24 29 27
1383
+ 27 27 34 34 36 38 38 38 36 36 40 36 37 38 45 45 36 34 33 31 31 34 34
1384
+ 33 33 28 28 27 23 24 11 11 10 10 18 25 21 20 17 17 17 20 15 24 18 24
1385
+ 26 23 23 18 20 25 23 30 30 30 33 33 37 37 32 37 37 32 45 35 37 37 37
1386
+ 40 36 49 49 36 36 34 33 20 15 9 9 8 7 12 22 21 28 28 30 33 36 36 36 34
1387
+ 31 31 25 31 28 26 26 24 20 17 9 11 8 9 10 23 23 31 23 23 15 9 9 15 33
1388
+ 26 33 33 31 25 25 22 31 24 23 12 10 12 11 9 8 9 7 7 8 8 9 18 12 9 9 18
1389
+ 20 26 31 21 21 9 8 8 11 13 21 21 23 15 15 15 15 15 17 17 9 7 9 19 20
1390
+ 21 21 25 25 25 25 25 23 23 9 9 9 21 16 24 24 24 24 26 33 33 33 31 31
1391
+ 27 15 17 7 4 4 4 16 20 27 33 34 34 23 15 14 8 9 6 6 9 1 14 16 8 11 15
1392
+ 23 25 34 36 31 33 16 16 6 6 6 9 8 14 9 11 11 9 13 13 10 8 10 9 9 7 8
1393
+ 20 20 20 14 14 10 10 10 16 8 8 6 6 8 9 10 7 8 8 8 8 6 8 6 6 8 12 9 8 7
1394
+ 13 10 8 8 9 8 8 8 9 6 6 6 6 8 6 6 6 6 6 10 11 10 12 12 10 7 6 6 7 6 6
1395
+ 7 8 7 6 6 7 6 8 6 6 8 8 8 8 6 6 6 8 6 6 8 6 6 11 15 9 9 9 9 9 9 9 9 9
1396
+ 9 8 9 9 6 6 6 7 9 6 6 6 7 7 7 8 9 7 7 9 9 11 8 11 11 11 9 10 9 8 8 8
1397
+ 10 6 6 9 8 6 6 6 6 8 8 9 11 9 8 8 8 8 8 8 8 9 9 9 10 10 8 6 6 8 6 6 10
1398
+ 11 10 6 6 8 6 6 6 6 8 11 8 9 8 8 8 8 8 9 6 6 9 8 8 8 6 6 13 15 12 12 8
1399
+ 8 6 6 8 9 8 8 9 8 8 6 6 6 8 8 6 6 6 8 8 8 6 6 8 6 6 8 6 6 9 8 8 6 6 8
1400
+ 8 6 6 8 6 7 8 9 10 11 11 10 9 9 8 10 9 10 10 8 9 13 8 8 8 13 13 11 7 7
1401
+ 7 11 8 8 10 11 10 9 9 14 7 7 10 10 10 8 8 8 8 6 7 8 8 6 6 6 6 6 6 6 6
1402
+ 6 8 9 10 8 8 6 6 8 8 8 8 8 8 8 8 8 8 6 6 8 8 9 10 10 4 4 4 6 7 6 6 6 6
1403
+ 10 8 8 9 15 11 6 6 8 8 8 8 8 8 9 9 8 9 7 7 8 6 6 8 13 10 8 8 7 6 8 6 6
1404
+ 8 6 7 8 8 8 8 8 8 8 4
1405
+
1406
+
1407
+ Sequence : 22ak93c2.r1t
1408
+ Is_read
1409
+ Padded
1410
+ Staden_id 11
1411
+ Clipping QUAL 39 331
1412
+ Align_to_SCF 1 43 1 43
1413
+ Align_to_SCF 44 317 45 318
1414
+ Align_to_SCF 319 716 319 716
1415
+ SCF_File 22ak93c2.r1tSCF
1416
+ Primer Universal_primer
1417
+ Strand Reverse
1418
+ Dye Dye_terminator
1419
+ Template 22ak93c2
1420
+ Clone bK216E10
1421
+ Sequencing_vector "m13mp18"
1422
+ Seq_vec SVEC 1 38 "M13mp18"
1423
+ Tag ALUS 43 180
1424
+ Tag DONE 43 43 "AUTO-EDIT: deleted C at 43 (terminator, isolated, strong)"
1425
+ Tag DONE 254 254 "AUTO-EDIT: replaced T by g at 254 (terminator, isolated, strong)"
1426
+ Tag ALUS 269 402
1427
+ Tag DONE 283 283 "AUTO-EDIT: replaced T by g at 283 (terminated, isolated, strong)"
1428
+ Tag AMBG 298 302 "AUTOEDIT: Check this edit cluster!"
1429
+ Tag DONE 317 317 "AUTO-EDIT: replaced C by a at 317 (terminated, compound, strong)"
1430
+ Tag DONE 318 318 "AUTO-EDIT: inserted g at 318 (terminated, compound, strong)"
1431
+
1432
+ DNA : 22ak93c1.r1ta
1433
+ GGGGGGATGATACAATTCGAGCTCGGTACCTTCAAGCGATTCTCCTGCCTCAGCCTCCCG
1434
+ AGTAGCTGGGATTATAAACTGTGCGTGCGCCACCATGCCTGGCTAATTTTTGTATTTTTA
1435
+ GTAGGGATGGGGTTTCACCATGTTGGCCAaGCTGGTCTCGAGCTCCTGACCTAGGATtAC
1436
+ AGGCCTAaGCCACCGCACCCGGCATGATGGGTCTTTATTCTTCAAAGCAgGAGGAAGGGA
1437
+ TCCTAGAAAAACAGAGACAAgGCCAAACATGGTAGCTCACACCTGTAATCCCAGCACTTT
1438
+ GGGAgGCCAgTGCGGGTGAATCACgAAGTCAGGAGTTCAAGACCATCCTGGCCAACATGG
1439
+ TGTAACACCGTCTCTCCTAGGATAAACCGAAATCACCTGGGTGTCCTGGCAAGTGCCTGT
1440
+ CATCCCTGACCACTTGGAAAGCTGAGCCGAATCCTTATCCCnGAAAGTAAGGTTACGTGA
1441
+ ACCCACATCAAACCACTGCGCTCCAGCCCAAAACATCGTGTTATACTCTGTCGCGACATT
1442
+ GATGCCCAAAAAACAGGACCGCTATTGGGAGACACTCCCGGGTAATTGGGATTGAGGAGG
1443
+ TTAAAGAAAACTCTTGATCTGAGAAAAAAGACCCTATCCTGGCGAGAAAACCACATGTGG
1444
+ ACCACACCGCTTCACTTTGTCTAAGCAAAAAACGCCCCACTTGAAGATGACCCCCCCAGC
1445
+ TGAAATCTTCCATTAATCCCCGAACATAAAGGTTTAACCCAAAATTTCCTGTTAGACCGC
1446
+ CTTCTGAAGTTTCTAAAATTCTATGGGTAGGGACAAAAGTCCCTCGTTGAATTTCTAGGA
1447
+ TACTTCCAAATATCCCCCACCTCTCCCCTGGGATCCCGATAGCTTTCTTGTCCCTAAAAA
1448
+ AAACTTTGTTCAAACGGAGAAACCACACCCGGGGAAAATTCCCCTTTTCTTGGGGTTTAG
1449
+ GTTTCTTTAAATAAATGCTGGGCACCAATCCTCAAATGAGTTCTTACCCAATCCCATATG
1450
+ TTTTTAATCCTCAACAACAACCTTATTTCCTTAAGAGACCTCTCCTCGGAAATCATTTCA
1451
+ AAACAAAAAAAACACTTAAGCCTTGGGTCTTTGGGACCCCCCAAAAAAAAAACATAAAGG
1452
+ GGTGGCACCGTATACGCCTTGCCCATCTTTCTAGTGGAAGATAACGAAACGGTAGCTGGG
1453
+ GCCAACCAGGCATCAGTGGGGGATACCGCTAAATGTTGGGGAACACCCAAAAACCCATCT
1454
+ GCAGGCACCGCGAATGCGGAAAAGCGCACGCTATCACAAATGGTATATGGGCACC
1455
+
1456
+ BaseQuality : 22ak93c1.r1ta
1457
+ 8 10 8 9 16 9 9 9 11 8 8 9 10 11 11 10 19 24 26 24 26 22 21 17 13 16
1458
+ 11 11 10 10 14 14 19 22 24 26 32 32 33 29 29 29 27 27 27 27 27 32 32
1459
+ 25 27 20 25 19 32 27 29 32 32 32 34 34 21 23 12 14 14 27 32 32 29 28
1460
+ 27 34 31 34 34 34 37 34 32 32 30 30 28 28 28 30 30 33 33 33 30 30 34
1461
+ 34 34 37 40 38 37 34 32 34 32 37 32 37 37 28 28 28 33 33 33 33 32 30
1462
+ 30 29 28 21 23 14 12 30 32 45 45 41 41 30 25 22 33 33 34 23 21 18 12
1463
+ 12 11 13 27 27 23 23 11 11 9 10 11 18 18 30 15 20 12 11 10 20 12 14 14
1464
+ 32 32 33 28 28 18 23 18 12 12 20 12 16 13 18 14 15 12 32 23 21 10 10 9
1465
+ 9 10 16 18 21 9 9 9 18 21 14 14 12 10 14 14 23 27 24 19 19 24 28 30 29
1466
+ 29 34 34 29 25 17 12 12 16 14 14 23 10 10 10 11 15 21 24 18 25 11 11
1467
+ 15 32 28 34 34 29 32 32 37 37 34 34 31 28 28 25 22 17 14 11 10 10 8 8
1468
+ 9 14 8 8 12 17 18 15 13 18 17 17 10 10 10 22 16 24 30 24 28 27 32 19
1469
+ 12 12 12 30 21 17 9 9 9 9 17 16 24 24 23 20 12 12 7 7 7 14 13 10 10 10
1470
+ 23 23 22 15 17 18 24 24 15 17 11 10 8 8 8 13 20 25 17 17 17 20 20 18
1471
+ 10 11 12 12 11 25 10 12 12 9 8 8 8 8 8 12 14 9 9 9 9 10 10 8 8 11 9 9
1472
+ 9 14 20 20 19 15 15 15 15 11 11 6 6 9 8 10 12 12 15 15 10 9 10 11 7 7
1473
+ 9 11 14 11 11 10 11 9 14 14 23 19 18 14 12 10 10 10 10 10 16 11 11 9 8
1474
+ 8 9 8 11 11 9 8 8 10 9 12 13 9 9 8 8 8 8 8 6 6 11 6 6 8 11 9 9 9 6 6
1475
+ 12 9 9 6 6 8 11 9 8 6 7 9 9 7 8 4 4 4 6 8 6 6 8 8 6 6 8 6 8 12 12 14
1476
+ 10 9 10 11 9 8 7 7 7 7 10 8 8 6 6 8 6 6 8 10 8 6 6 10 8 10 9 10 10 9 8
1477
+ 8 6 6 6 6 8 8 8 9 9 9 9 7 7 9 9 10 10 10 13 12 9 9 9 9 7 8 8 8 9 8 8 9
1478
+ 9 9 8 6 6 8 6 6 6 7 7 9 9 10 8 8 7 7 7 9 10 10 8 6 6 6 6 6 10 10 8 6 6
1479
+ 8 8 8 7 9 7 10 7 11 8 8 8 8 9 9 9 8 8 6 6 10 11 16 8 7 7 7 6 8 6 6 8
1480
+ 10 11 9 9 9 7 7 8 7 7 7 7 7 8 11 9 16 16 24 22 15 9 8 13 13 12 12 9 9
1481
+ 9 9 9 7 7 7 7 7 7 7 8 7 7 7 6 9 11 9 8 8 9 9 9 7 9 10 14 12 9 9 8 8 6
1482
+ 6 6 6 8 6 6 8 6 6 6 6 6 9 8 10 10 9 10 10 13 10 11 6 7 11 6 6 8 6 6 6
1483
+ 6 6 6 9 9 7 7 8 8 8 8 8 9 8 9 13 11 8 6 6 6 9 9 8 8 8 8 8 8 8 11 8 7
1484
+ 10 12 7 7 9 8 6 6 8 8 7 7 10 8 8 7 7 7 8 8 8 8 7 7 7 7 7 10 9 10 12 10
1485
+ 7 7 6 9 6 6 6 6 7 9 7 7 4 10 9 7 6 8 6 6 8 6 6 9 6 7 7 10 10 10 10 13
1486
+ 13 8 9 8 8 8 6 7 10 11 10 9 8 6 6 6 6 6 8 8 7 9 7 7 7 7 7 7 7 10 10 10
1487
+ 8 8 8 7 7 8 8 8 8 6 6 7 7 7 9 8 6 8 6 6 6 8 8 8 7 9 9 12 15 9 8 7 9 8
1488
+ 8 9 8 8 6 8 12 11 7 7 7 7 7 7 9 11 11 9 9 9 10 9 9 9 8 8 8 8 8 7 7 12
1489
+ 13 13 15 11 11 7 7 7 9 11 10 12 6 6 6 6 8 7 7 7 8 10 10 10 10 13 9 12
1490
+ 11 11 6 6 6 6 8 8 6 7 7 12 10 13 9 8 7 7 10 7 7 15 17 15 12 17 20 15
1491
+ 13 15 6 6 6 6 8 8 6 6 6 7 7 7 10 7 7 10 11 8 8 5 5 8 8 12 12 8 10 6 6
1492
+ 6 6 8 8 7 7 7 12 10 7 7 9 7 7 9 9 8 9 10 10 8 8 9 11 9 8 9 9 11 11 9 9
1493
+ 8 6 8 6 6 6 6 6 6 6 6 8 8 8 8 12 6 6 6 6 8 6 7 7 6 8 6 6 8 8 10 11 10
1494
+ 12 7 8 8 9 12 6 6 6 6 6 6 6 6 8 9 9 9 9 9 8 7 8 7 7 7 8 7 7 7 7 9 9 9
1495
+ 9 6 6 6 6 14 8 8 8 8 8 6 6 7 7 7 11 9 7 6 6 6 6 10 6 6 6 8 8 7 8 7 7 6
1496
+ 7 7 8 8 12 13 15 15 21 9 10 9 8 8 10 6 6 6 6 4 4 4 8 8 8 7 6 6 6 6 6 6
1497
+ 6 6 6 6 6 9 9 9 6 6 6 10 6 10 15 20 12 9 8 6 6 6 6 6 8 8 6 6 6 6 8 12
1498
+ 13 12 10 9 8 11 8 10 9 7 7 4 4 6 6 8 4 4 4 6 8 6 6 6 8 8 13 10 8 9 9 9
1499
+ 7 7 9 10 8 8 6 6 6 6 6 6 10 11 10 10 10 7 7 8 6 6 8 6 6 8 10 13 8 11 8
1500
+ 11 10 6 6 6 7 11 14 11 11 6 6 8 8 8 9 9 9 9 7 7 10 10 6 6 6 6 6 6 8 8
1501
+ 8 7 7 7 8 8 11 11 11 7 6 8 6 6 8 8 6 6 10 10 13 8 8 6 6 6 8 9 9 6 6 9
1502
+ 9 8 8 6 6 8 6 6 8 8 4
1503
+
1504
+
1505
+ Sequence : 22ak93c1.r1ta
1506
+ Is_read
1507
+ Padded
1508
+ Staden_id 12
1509
+ Clipping QUAL 31 335
1510
+ Align_to_SCF 1 1315 1 1315
1511
+ SCF_File 22ak93c1.r1taSCF
1512
+ Primer Universal_primer
1513
+ Strand Reverse
1514
+ Dye Dye_terminator
1515
+ Template 22ak93c1
1516
+ Clone bK216E10
1517
+ Sequencing_vector "m13mp18"
1518
+ Seq_vec SVEC 1 30 "M13mp18"
1519
+ Tag ALUS 31 172
1520
+ Tag DONE 150 150 "AUTO-EDIT: replaced T by a at 150 (terminator, isolated, strong)"
1521
+ Tag DONE 178 178 "AUTO-EDIT: replaced G by t at 178 (terminator, isolated, strong)"
1522
+ Tag DONE 188 188 "AUTO-EDIT: replaced T by a at 188 (terminator, isolated, strong)"
1523
+ Tag DONE 230 230 "AUTO-EDIT: replaced T by g at 230 (terminator, isolated, strong)"
1524
+ Tag DONE 261 261 "AUTO-EDIT: replaced C by g at 261 (terminated, isolated, strong)"
1525
+ Tag ALUS 262 446
1526
+ Tag DONE 305 305 "AUTO-EDIT: replaced T by g at 305 (terminated, isolated, strong)"
1527
+ Tag DONE 310 310 "AUTO-EDIT: inserted g at 310 (terminated, compound, strong)"
1528
+ Tag DONE 325 325 "AUTO-EDIT: replaced A by g at 325 (terminated, isolated, strong)"
1529
+
1530
+ DNA : 22am91h5.r1t
1531
+ GGGnnATAGATACGATCCGAGCTCGGTACCCATGTTGGCCAAGCTGGTCTCGAGCTCCTG
1532
+ ACCTAGGATTACAGGCCTAAGCCACCGCACCCGGCATGATGGGTCTTTATTCTTCAAAGC
1533
+ AGGAGGAAGGGATCCTAGAAAAACAGAGACAAGGCCAAACATGGTAGCTCACACCTGTAA
1534
+ TCCCAGCACTTTGGGAGGCCAGTGCGGGTGAATCACGAAGTCAGGAGTTCAAGACCAGCC
1535
+ TGGCCAACATGGTGAAACACCGTCTCTACTAAAATAAAAAGAAATTAGCTGGGTGTCGTG
1536
+ GCAGGTGCCTGTAATCCCAGCCACTTGGGAAGCTGAGGCAGGAGAATCGCTTGAACCCGG
1537
+ GAGGTGGAGGTTGCAnTGAGCCGAGATCACGCGACTGCACTCCAGCCCAACCAATAATGT
1538
+ GAGACTCTGTCTCGAnAAnnnAAAGCnGAAAACAGACnnCTAGTACnGTACTTAACGGGG
1539
+ TAATTATAATAATTAAATGAAAAAAATAnCTGTGAGGTGATTGATATAnTGCTGTTGCTA
1540
+ AATACAACTAACATTTTAATATACCGGTGGAGTGTTTCTAATCTGAnAATCCAAATTAGA
1541
+ AATGCTCACAGTCTGAAACTTTTTGAGCACCAACTAATGTTCAAAGAATTGCTATGGACC
1542
+ ATATGGGTGGTTGATTTT
1543
+
1544
+ BaseQuality : 22am91h5.r1t
1545
+ 4 8 4 4 4 4 4 7 10 9 10 15 10 7 7 10 10 9 19 20 33 34 34 34 36 36 33
1546
+ 33 31 33 33 32 31 31 26 26 27 23 30 26 26 26 26 34 34 38 38 38 37 45
1547
+ 45 37 37 37 37 45 45 45 45 37 37 37 45 45 32 18 10 21 18 28 29 33 33
1548
+ 33 33 45 45 42 42 37 33 33 33 36 33 33 30 30 30 30 32 36 33 33 33 33
1549
+ 33 33 37 37 45 45 37 37 37 33 33 35 35 35 35 43 35 42 42 35 35 34 34
1550
+ 34 29 29 22 27 27 27 37 27 37 45 33 30 30 37 28 32 32 10 10 21 32 32
1551
+ 34 27 23 12 11 9 9 9 18 21 28 31 33 30 30 30 30 34 34 34 34 31 33 34
1552
+ 34 33 31 33 33 33 33 33 33 45 45 40 37 37 37 33 33 30 30 30 30 33 30
1553
+ 33 26 26 26 25 23 18 14 12 20 23 27 25 21 21 21 28 28 30 31 31 31 31
1554
+ 32 21 21 12 6 6 9 9 14 17 19 17 10 16 13 8 8 11 13 9 13 12 14 14 21 23
1555
+ 26 34 36 34 31 22 24 20 28 28 33 27 28 25 27 24 34 32 23 12 12 12 23
1556
+ 23 33 28 34 34 37 32 32 15 11 11 32 32 40 37 38 18 11 9 9 18 25 45 34
1557
+ 28 28 9 9 20 29 45 38 34 23 23 10 23 23 33 33 33 21 21 13 25 25 25 29
1558
+ 27 33 30 33 33 34 33 30 30 19 19 30 30 30 26 26 30 27 27 23 19 15 24
1559
+ 27 28 21 9 9 9 19 14 12 10 12 12 13 17 28 18 19 21 24 24 25 24 24 24
1560
+ 24 18 18 13 18 8 8 8 10 10 10 7 7 7 8 8 12 15 4 4 4 25 18 21 17 15 16
1561
+ 14 9 9 17 14 17 9 17 8 8 10 10 10 11 17 17 22 24 23 24 24 19 13 10 8
1562
+ 15 15 10 9 9 9 16 12 12 14 19 18 26 24 22 22 24 25 21 24 24 32 19 20 9
1563
+ 9 4 4 4 4 4 4 4 4 17 16 16 4 4 4 7 10 8 7 6 13 8 8 4 4 4 4 8 8 7 7 7 4
1564
+ 4 4 17 23 12 9 8 7 7 8 7 10 10 8 6 6 6 6 6 10 10 7 7 7 7 9 7 8 10 7 7
1565
+ 8 6 6 8 8 8 9 10 7 12 4 4 4 9 9 7 6 6 7 6 6 10 9 7 8 10 7 7 7 9 4 4 4
1566
+ 14 8 8 9 7 6 6 8 8 8 7 7 6 6 9 7 7 10 13 8 9 8 8 8 14 7 7 7 7 9 8 12
1567
+ 12 4 4 4 7 7 7 7 8 8 8 7 7 7 7 7 7 8 10 8 6 6 6 4 4 4 8 12 9 9 17 9 7
1568
+ 7 7 7 6 6 6 6 8 10 6 6 6 8 6 6 6 6 6 6 6 6 6 6 6 6 6 13 11 9 8 7 8 7 7
1569
+ 7 8 7 9 8 7 6 6 4 6 6 8 7 7 7 6 8 6 6 8 7 7 9 9 10 11 7 6 6 6 6 6 12 9
1570
+ 8 8 12 11 10 8 7 7 4 4 4 7 7 8 4
1571
+
1572
+
1573
+ Sequence : 22am91h5.r1t
1574
+ Is_read
1575
+ Padded
1576
+ Staden_id 20
1577
+ Clipping QUAL 32 361
1578
+ Align_to_SCF 1 678 1 678
1579
+ SCF_File 22am91h5.r1tSCF
1580
+ Primer Universal_primer
1581
+ Strand Reverse
1582
+ Dye Dye_terminator
1583
+ Template 22am91h5
1584
+ Clone bK216E10
1585
+ Sequencing_vector "m13mp18"
1586
+ Seq_vec SVEC 1 31 "M13mp18"
1587
+ Tag ALUS 153 443
1588
+
1589
+ DNA : 22aq40a8.s1
1590
+ AAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCACAGAGACAAGGCCAAACATGG
1591
+ TAGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAGTGCGGGTGAATCACGAGGTCAG
1592
+ GAGTTCAAGACCAGCCTGGCCAACATGGTGAA-CACCGTCTCTACTAAAATAAAAAGAAA
1593
+ TTAGCTGGGTGTCGTGGCAGGTGCTtGTAATCCCAGCCACTTGGGAAGCTGAGGCAGGAG
1594
+ AATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCGACTGCACTCCA
1595
+ GCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGACAAGACAACTAG
1596
+ TACAGTACTTACAGGGTTATTATGATGATTAnATGAGAGAATAGCTGTGAGGTGATTGAT
1597
+ ATAGTGCTGTGCTTAAATACAACTATCAATTTTATTATACGGGGTTGAGTGTTTCCAATC
1598
+ TGAAAATCCCAAATTTGGAATGCTCTACGGTCTGAAACCTTTTTTGAGCACCGACCTAAA
1599
+
1600
+ BaseQuality : 22aq40a8.s1
1601
+ 7 9 10 11 11 23 27 25 25 9 9 9 12 14 23 27 24 21 21 16 22 22 22 24 23
1602
+ 23 23 30 30 30 30 30 32 34 31 31 34 34 28 28 28 30 30 30 28 28 28 34
1603
+ 34 34 34 34 34 37 34 34 34 34 34 34 37 37 37 37 37 37 37 32 34 29 29
1604
+ 29 45 38 40 40 37 37 32 37 37 37 37 37 37 37 37 37 40 40 40 40 40 37
1605
+ 30 30 32 22 22 22 32 32 41 45 45 41 41 45 30 32 27 32 32 40 37 37 40
1606
+ 37 37 45 45 45 40 40 37 37 37 37 37 37 37 37 40 37 37 37 31 32 28 28
1607
+ 28 32 32 37 34 37 38 38 38 27 27 11 1 11 10 21 27 34 34 37 37 34 34 45
1608
+ 45 45 38 37 37 37 37 37 37 34 34 34 34 33 30 30 28 28 28 28 28 30 37
1609
+ 37 37 37 33 36 36 31 31 31 24 24 24 24 21 21 17 12 4 4 4 14 17 26 25
1610
+ 25 31 34 34 31 31 31 33 31 31 34 35 37 37 32 32 34 34 30 31 31 34 30
1611
+ 33 30 30 30 33 33 30 30 23 22 22 32 32 40 34 36 34 31 26 28 26 24 16 9
1612
+ 9 9 8 15 22 22 25 25 31 31 30 30 28 30 30 30 30 28 28 28 30 30 30 30
1613
+ 30 37 32 27 23 18 18 27 27 34 34 31 33 30 30 28 28 28 28 28 30 34 33
1614
+ 33 33 33 30 26 26 23 23 23 30 37 36 32 32 24 23 21 20 28 31 29 30 22
1615
+ 21 18 13 15 23 26 34 34 36 33 30 30 30 30 25 25 25 26 23 26 25 24 24
1616
+ 25 27 25 22 14 15 12 12 12 21 21 25 25 22 21 21 24 19 19 14 16 11 13
1617
+ 14 20 27 30 28 28 28 30 33 34 34 23 23 16 7 7 4 4 4 18 17 21 23 21 17
1618
+ 11 13 18 18 18 20 18 18 11 14 7 7 7 15 20 18 11 10 15 23 18 16 10 8 8
1619
+ 13 17 18 14 14 14 13 13 14 17 18 13 13 9 9 9 14 12 18 18 11 11 5 5 5 6
1620
+ 6 6 8 12 10 13 15 11 11 11 8 6 6 6 6 6 8 6 6 8 8 8 7 7 9 9 8 8 19 8 8
1621
+ 14 8 8 6 6 11 9 9 8 14 6 6 7 7 8 6 7 6 6 9 9 10 15 14 12 7 7 7 7 13 15
1622
+ 22 22 19 14 9 8 8 8 12 15 19 7 6 10 9 8 6 7 9 9 10 10 8 8 7 7 4 4
1623
+
1624
+
1625
+ Sequence : 22aq40a8.s1
1626
+ Is_read
1627
+ Padded
1628
+ Staden_id 3
1629
+ Clipping QUAL 39 388
1630
+ Align_to_SCF 1 152 1 152
1631
+ Align_to_SCF 154 540 153 539
1632
+ SCF_File 22aq40a8.s1SCF
1633
+ Primer Universal_primer
1634
+ Strand Forward
1635
+ Dye Dye_primer
1636
+ Template 22aq40a8
1637
+ Clone bK216E10
1638
+ Sequencing_vector "m13mp18"
1639
+ Seq_vec SVEC 1 38 "M13mp18"
1640
+ Tag ALUS 49 339
1641
+ Tag DONE 206 206 "AUTO-EDIT: replaced N by t at 206 (terminated, compound, strong)"
1642
+
1643
+ DNA : 22an02b10.s1t
1644
+ CGTGCCTGCAGGTCGACTCTAGAGGATCCCCCCTGTAATCCCAGCACTTTGGGAGGCCAg
1645
+ TGCGGGTGAATCACgATGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACACCG
1646
+ TCTCTACTAAAATAAAAAGAAATTAGCTGGGTGTCgTGGCAGGTGCCTGTAATCCCAGCC
1647
+ ACTTGGGAAGCTGAgGCAGGAGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCC
1648
+ GAgATCACGCGACTGCACTCCAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAA
1649
+ AAAGCAGAGACAAGACAACTAGTACAGTACTTACaGGGTTATTATGATGATTAAATGAGA
1650
+ gAATAGCTGTGAgGTGATTGATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATA
1651
+ CGGGTTGAGTGTATCTAATCTGAAAATCCAAAATCAGAAATGCGCTACAGTCTGAAACTT
1652
+ TTTTGAGCACCGACCTAATGTTCCAAGGAAGTGCTTATCGGAGCACCATGGGTCGGTACA
1653
+ CATTTGGGTCGGGAATATnCCACCAGTGAGTACCATAAAATGCCAATATCCCCTCCCACC
1654
+ CCCAACA
1655
+
1656
+ BaseQuality : 22an02b10.s1t
1657
+ 7 7 9 12 10 15 16 17 13 13 21 18 23 17 28 28 37 37 37 33 26 26 17 19
1658
+ 12 21 25 36 45 45 34 34 34 34 34 37 37 37 37 37 32 25 9 9 13 17 17 14
1659
+ 18 18 21 29 32 19 18 18 18 21 9 9 9 23 19 30 30 37 29 37 37 32 34 23
1660
+ 20 13 13 10 13 22 32 21 17 17 23 18 10 10 10 12 13 9 9 14 23 29 28 22
1661
+ 22 32 33 37 34 34 34 34 34 34 34 30 37 32 37 37 41 34 33 33 33 21 21
1662
+ 14 14 14 24 28 41 37 32 32 21 21 21 25 30 30 37 30 37 30 30 30 32 36
1663
+ 28 27 17 27 27 37 32 37 36 37 25 23 10 10 10 32 21 23 10 15 10 23 23
1664
+ 36 32 32 34 34 34 37 37 25 25 13 9 9 21 25 25 32 32 32 41 32 28 28 11
1665
+ 22 22 32 32 13 13 10 11 9 10 10 12 12 14 32 32 29 22 22 21 21 17 21 27
1666
+ 41 41 41 28 27 32 27 27 29 37 32 34 17 15 14 28 23 25 20 9 9 9 25 10
1667
+ 12 12 14 13 10 10 9 16 13 23 23 14 14 22 25 34 28 28 22 28 28 28 19 18
1668
+ 10 10 11 19 21 28 28 28 30 30 30 23 18 10 10 28 32 32 34 28 28 28 28
1669
+ 28 31 34 34 25 25 12 19 11 14 14 25 21 24 24 18 18 18 22 23 13 11 10
1670
+ 10 10 10 14 13 10 9 9 7 8 14 17 16 10 10 13 16 15 18 18 18 18 14 15 12
1671
+ 12 9 12 9 12 12 13 9 14 10 18 19 14 9 13 11 18 14 15 15 16 20 19 18 14
1672
+ 9 8 8 6 6 6 11 9 9 14 17 20 12 13 6 6 8 8 11 10 21 23 15 14 13 13 14
1673
+ 21 27 28 32 29 34 28 27 23 14 10 10 12 17 15 13 11 11 11 18 28 33 24
1674
+ 18 14 12 17 12 12 18 22 19 19 24 28 20 21 19 19 20 17 17 26 25 20 9 14
1675
+ 7 7 7 15 21 33 27 27 18 20 19 15 15 20 18 14 14 8 8 9 9 9 7 7 7 7 7 8
1676
+ 9 9 12 7 7 9 11 10 11 13 13 11 7 7 8 15 17 26 25 25 27 25 24 21 19 20
1677
+ 18 13 13 14 9 9 14 18 17 12 15 16 21 20 18 8 7 6 6 6 13 20 25 18 11 14
1678
+ 9 9 9 11 11 12 12 15 15 18 10 10 8 8 10 10 9 9 9 16 11 7 7 6 6 8 4 4 4
1679
+ 4 4 6 10 10 13 13 12 6 6 6 6 12 15 12 11 11 4 4 4 6 6 6 6 6 8 6 6 6 8
1680
+ 6 8 11 13 8 8 6 6 6 6 9 16 9 13 13 17 14 13 9 7 7 12 8 8 8 9 9 9 9 9 9
1681
+ 8 8 8 10 9 7
1682
+
1683
+
1684
+ Sequence : 22an02b10.s1t
1685
+ Is_read
1686
+ Padded
1687
+ Staden_id 21
1688
+ Clipping QUAL 32 446
1689
+ Align_to_SCF 1 607 1 607
1690
+ SCF_File 22an02b10.s1tSCF
1691
+ Primer Universal_primer
1692
+ Strand Forward
1693
+ Dye Dye_terminator
1694
+ Template 22an02b10
1695
+ Clone bK216E10
1696
+ Sequencing_vector "m13mp18"
1697
+ Seq_vec SVEC 1 31 "M13mp18"
1698
+ Tag ALUS 32 301
1699
+ Tag DONE 60 60 "AUTO-EDIT: inserted g at 60 (terminated, compound, strong)"
1700
+ Tag DONE 75 75 "AUTO-EDIT: replaced A by g at 75 (terminated, isolated, strong)"
1701
+ Tag DONE 156 156 "AUTO-EDIT: replaced C by g at 156 (terminated, isolated, strong)"
1702
+ Tag DONE 195 195 "AUTO-EDIT: replaced A by g at 195 (terminated, isolated, strong)"
1703
+ Tag DONE 243 243 "AUTO-EDIT: replaced T by g at 243 (terminated, isolated, strong)"
1704
+ Tag DONE 335 335 "AUTO-EDIT: replaced T by a at 335 (all_stranded, isolated, strong)"
1705
+ Tag DONE 361 361 "AUTO-EDIT: replaced C by g at 361 (terminated, isolated, strong)"
1706
+ Tag DONE 373 373 "AUTO-EDIT: replaced C by g at 373 (terminated, isolated, strong)"
1707
+
1708
+ DNA : 22ak67d6.r1t
1709
+ CGCGCCCCTCnGACTCCGGCCCTAnCACGGTACCCACTTTGGGAGGCCAGTGCGGGTGAA
1710
+ TCACGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACACCGTCTCTACTAA
1711
+ AATAAAAAGAAATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAGCCACTTGGGAAG
1712
+ CTGAgGCAGGAGAATCGCTTGAACCCGGGAgGTGGAGGTTGCAGTGAGCCGAGATCACGC
1713
+ GACTGCACTCCAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGA
1714
+ TCAGATCACTAGTACAGTACTTACGGGCTTATTATGATGATTAAATGAGAGAATACCTGT
1715
+ GAGGTGATTGATATATTGCTGTGCTTAATACAnACTATCATTTTATTATACGGGTCGAGT
1716
+ GTTTCTAATCTGACAATCCAAATTAGAAATGCTCTACAGTCTGAAACCTTTTTGAGCACC
1717
+ CACCTACTGTTCAAAGGACGTGCTTATTGGAGCATTATGGGTTGTAACATTTTTGGGTTG
1718
+ GGAATTTTCACCCATTAGTACTATAAATGCCATTTTCCAAAAAATCTGAATCTAAAATTT
1719
+ CTGGTCCTACCAGCATTTGCTAGGGATCCCACCCGTCTTCTTCCTTACCTGTTTAAGTCT
1720
+ TATAAATC
1721
+
1722
+ BaseQuality : 22ak67d6.r1t
1723
+ 6 6 6 4 8 11 15 8 4 4 4 4 8 6 6 9 7 8 9 8 4 4 4 4 4 4 10 9 9 21 19 33
1724
+ 30 33 30 30 30 28 28 28 23 23 20 11 11 16 12 13 17 21 25 28 33 33 33
1725
+ 38 34 34 34 33 30 22 22 12 14 21 10 12 21 21 16 14 11 13 13 14 21 21
1726
+ 21 19 15 18 23 23 25 30 28 33 38 38 33 33 30 30 30 30 30 30 30 37 37
1727
+ 45 45 33 33 33 33 33 33 40 42 49 42 40 40 40 34 34 31 35 34 35 37 42
1728
+ 42 37 32 19 9 9 11 23 19 28 30 30 28 38 45 37 37 37 32 32 24 34 34 30
1729
+ 30 26 21 20 11 27 27 40 34 30 30 28 30 30 30 23 18 17 9 9 18 16 16 24
1730
+ 24 24 23 21 18 11 11 18 25 25 16 9 7 9 15 11 8 7 9 7 7 8 16 16 27 28
1731
+ 25 20 16 10 10 9 13 8 8 7 12 4 4 4 14 6 6 6 7 9 6 7 7 7 7 16 10 20 15
1732
+ 13 11 9 9 8 8 8 15 8 10 10 17 14 9 9 19 15 15 7 7 12 20 19 25 25 22 18
1733
+ 17 7 9 12 20 21 25 21 21 20 22 28 32 30 25 25 12 12 18 32 32 36 49 49
1734
+ 33 33 24 20 13 12 16 18 20 14 25 23 25 19 12 11 8 8 4 4 4 4 4 8 8 6 4
1735
+ 6 8 15 17 21 19 15 8 8 8 8 10 7 7 13 9 7 11 14 6 6 4 4 4 6 12 8 13 17
1736
+ 21 18 19 19 20 18 18 15 10 15 14 13 14 8 8 8 9 8 8 10 10 8 8 15 17 17
1737
+ 17 17 14 13 19 17 16 12 10 14 15 17 15 14 8 8 7 7 15 19 18 12 11 16 12
1738
+ 15 11 10 13 13 11 4 4 4 10 12 12 18 12 14 9 10 9 10 21 22 18 14 6 6 6
1739
+ 6 6 8 4 4 4 8 8 7 8 7 9 14 7 8 7 10 16 31 17 14 4 4 4 10 11 8 10 13 17
1740
+ 13 8 8 9 8 8 12 12 14 14 7 7 7 7 7 7 7 8 9 13 11 10 7 10 7 6 6 10 8 8
1741
+ 23 23 12 7 7 7 7 7 10 10 18 6 6 6 6 6 6 7 7 7 6 6 6 7 6 6 4 4 4 4 8 11
1742
+ 18 15 23 11 9 8 7 7 7 7 7 7 8 15 18 8 10 10 12 8 6 8 6 6 6 7 7 12 10
1743
+ 12 18 11 12 10 19 19 15 15 10 10 9 7 11 7 8 9 10 7 7 6 6 6 6 4 6 7 7 7
1744
+ 7 9 9 9 13 13 9 7 7 7 9 7 7 7 6 6 6 6 8 8 9 8 11 8 7 6 6 6 6 9 7 8 7 7
1745
+ 6 8 6 6 9 10 12 10 13 12 6 6 6 6 6 7 6 6 7 7 7 11 13 11 9 8 6 6 8 6 6
1746
+ 8 12 14 8 8 8 6 13 8 8 8 6 7 8 7 8 9 8 10 6 6 10 11 17 20 16 11 11 7 6
1747
+ 8 8 9 8 6 12 10 7 7 8 8 4
1748
+
1749
+
1750
+ Sequence : 22ak67d6.r1t
1751
+ Is_read
1752
+ Padded
1753
+ Staden_id 19
1754
+ Clipping QUAL 30 292
1755
+ Align_to_SCF 1 668 1 668
1756
+ SCF_File 22ak67d6.r1tSCF
1757
+ Primer Universal_primer
1758
+ Strand Reverse
1759
+ Dye Dye_terminator
1760
+ Template 22ak67d6
1761
+ Clone bK216E10
1762
+ Sequencing_vector "m13mp18"
1763
+ Tag ALUS 35 291
1764
+ Tag DONE 185 185 "AUTO-EDIT: replaced C by g at 185 (terminated, isolated, strong)"
1765
+ Tag DONE 211 211 "AUTO-EDIT: replaced T by g at 211 (terminated, isolated, strong)"
1766
+
1767
+ DNA : 22ak64b1.s1t
1768
+ TGGGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAACACCGTCTCTACTAAA
1769
+ ATAAAAAGAAATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAGCCACTTGGGAAGC
1770
+ TGAGGCAGGAGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAgATCACGCG
1771
+ ACTGCACTCCAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGAC
1772
+ AAGAcCACTAGTACAGTACTTACAGGGTtATTATGATGATTAAATGAGAGAATAGCTGTG
1773
+ AGGTGATTGATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATACGGGTTGAGTG
1774
+ TTTCTAATCTGAaAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACC
1775
+ GACCTAAATGTTCAAAGGGAGGTGCTTAATTGGAGCATTATGGGTTGTTAGATCTTTGGG
1776
+ TTGGGAATATTCAACC
1777
+
1778
+ BaseQuality : 22ak64b1.s1t
1779
+ 4 4 8 11 13 20 16 16 16 19 24 32 31 37 37 35 34 29 32 18 32 24 33 33
1780
+ 37 37 37 23 32 11 10 21 17 37 34 34 45 45 45 45 32 29 26 26 28 30 30
1781
+ 33 45 45 49 49 45 43 45 45 45 45 45 37 45 45 45 45 45 45 45 37 37 37
1782
+ 38 32 40 37 41 37 45 32 45 45 44 37 45 30 45 45 33 33 33 33 33 22 37
1783
+ 37 45 45 45 40 37 34 37 37 33 32 32 33 33 33 33 40 40 40 42 40 42 37
1784
+ 37 37 45 45 45 45 45 35 34 34 28 28 18 21 17 17 27 38 38 45 34 34 34
1785
+ 34 34 34 34 34 34 34 34 34 34 34 34 37 34 34 33 33 33 33 28 31 31 36
1786
+ 34 31 36 36 34 33 20 14 8 8 8 8 14 23 27 31 36 33 36 38 30 30 28 28 28
1787
+ 30 30 27 30 30 30 30 33 33 23 21 18 18 17 20 32 37 37 45 45 45 45 34
1788
+ 34 34 45 45 45 44 45 37 45 45 45 37 40 37 37 33 40 44 40 40 36 31 31
1789
+ 28 25 22 27 14 14 8 9 10 8 7 8 7 12 18 18 23 15 9 9 15 15 20 20 25 28
1790
+ 25 21 21 18 18 8 9 8 6 6 8 11 14 28 28 28 30 30 30 30 31 30 31 25 22
1791
+ 22 27 15 15 15 16 13 17 25 25 30 25 27 28 31 24 24 19 24 29 33 27 31
1792
+ 31 31 31 28 27 22 14 15 27 32 40 37 36 36 36 33 33 34 33 27 13 14 7 7
1793
+ 8 18 23 32 32 23 20 20 33 30 27 27 21 14 14 18 21 18 21 15 15 17 11 11
1794
+ 16 24 23 30 23 14 10 13 14 15 10 8 9 16 16 28 4 4 4 10 11 12 9 8 10 14
1795
+ 20 18 19 19 19 10 10 12 15 25 23 26 26 28 18 14 14 16 20 15 14 12 12
1796
+ 12 21 23 25 24 21 17 17 14 21 20 19 12 9 9 8 9 8 10 7 6 9 8 7 8 9 7 7
1797
+ 7 6 6 8 7 8 6 6 6 6 6 13 11 6 6 6 8 10 11 8 9 9 10 10 15 10 11 12 16
1798
+ 13 10 9 7 7 7 7 9 10 8 8 7 6 6 6 6 6 14 10 7 7 7 9 9 10 11 16 16 7 6 4
1799
+ 4 4 8 4
1800
+
1801
+
1802
+ Sequence : 22ak64b1.s1t
1803
+ Is_read
1804
+ Padded
1805
+ Staden_id 8
1806
+ Clipping QUAL 40 416
1807
+ Align_to_SCF 1 496 1 496
1808
+ SCF_File 22ak64b1.s1tSCF
1809
+ Primer Universal_primer
1810
+ Strand Forward
1811
+ Dye Dye_terminator
1812
+ Template 22ak64b1
1813
+ Clone bK216E10
1814
+ Sequencing_vector "m13mp18"
1815
+ Seq_vec SVEC 1 39 "M13mp"
1816
+ Tag CONF 44 44 "AUTOEDIT: possible compression"
1817
+ Tag CONF 96 96 "AUTOEDIT: possible compression"
1818
+ Tag DONE 172 172 "AUTO-EDIT: replaced A by g at 172 (terminated, isolated, strong)"
1819
+ Tag DONE 245 245 "AUTO-EDIT: replaced A by c at 245 (all_stranded, compound, strong)"
1820
+ Tag AMBG 246 246 "AUTOEDIT: Check this edit stack (conflict & possible misassembly))!"
1821
+ Tag DONE 269 269 "AUTO-EDIT: replaced C by t at 269 (all_stranded, isolated, strong)"
1822
+ Tag DONE 373 373 "AUTO-EDIT: replaced N by a at 373 (terminated, isolated, strong)"
1823
+
1824
+ DNA : 22ak81h10.s1t
1825
+ GTGGCATGCCTGCAGGTCAACTCTAAAAGATCCCCTGAAACACCGTCTCTACTAAAATAA
1826
+ AAAGAAATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAgCCACTTGGGAAGCTGAG
1827
+ GCAGGAGAATCGCTTGAACCCGGGAgGTGGAgGTTGCAGTGAGCCGAGATCACGCGACTG
1828
+ CACTCCAGCCCAAcCAATAGTGTGAGACTCTGTCTCGAaaAAaAAAAAGCAgAGACaAGA
1829
+ CCACTAGTACAGTACTTACAGGGTTATTATGATGATTAAaTGAGAGAATAGCTGTGAGGT
1830
+ GATTGATATAGTGCTGTGCTTAATACaAACTATCATTTTATTATACGGGTTGAGTGTTTC
1831
+ TAATCTGAAAATCCaaAATTAGAAATGCTCTACAGTCTGAAACTTTtTTGAGCACCGACC
1832
+ TACTGTGCACAGGAAGTGCTTATTGGAGCATGATGGGTTGTTACATATTTGGGTTGGGAA
1833
+ TATTCACCCAGTAAGTACTATAAAATGCAAATATTCCAACAAG
1834
+
1835
+ BaseQuality : 22ak81h10.s1t
1836
+ 6 6 6 6 6 8 9 16 11 13 21 20 22 22 22 15 14 9 9 9 9 15 18 15 9 9 9 9 9
1837
+ 15 23 36 36 36 36 34 38 40 30 28 28 28 30 32 37 37 36 38 37 44 45 45
1838
+ 49 49 45 45 37 37 38 45 45 45 38 37 37 38 38 37 37 34 34 34 32 32 38
1839
+ 45 49 45 45 45 45 37 33 33 27 27 18 18 30 32 36 36 36 36 36 36 36 34
1840
+ 32 21 18 4 4 4 18 17 27 28 33 36 36 36 49 36 36 36 34 34 45 27 27 30
1841
+ 25 27 12 20 20 34 34 38 37 37 34 34 25 25 18 18 21 29 38 45 21 28 14
1842
+ 14 14 27 21 34 18 17 17 32 27 25 22 13 13 10 18 15 32 32 32 37 28 22
1843
+ 22 21 14 10 10 11 23 22 28 20 14 14 8 8 11 20 20 16 12 16 16 28 21 19
1844
+ 10 10 10 19 18 20 20 14 14 32 32 34 32 25 18 18 18 18 27 32 32 24 24
1845
+ 23 23 14 10 10 10 9 9 10 11 12 12 12 12 17 11 11 11 10 8 6 6 8 8 10 16
1846
+ 16 16 11 14 10 9 9 9 17 11 11 11 14 15 22 18 14 14 11 11 15 27 22 19
1847
+ 23 27 30 28 22 18 16 11 10 10 11 9 10 11 11 12 15 20 27 27 23 21 14 10
1848
+ 10 10 10 14 22 25 22 14 14 22 32 38 34 34 34 31 24 18 14 12 9 10 10 23
1849
+ 18 24 16 16 18 18 18 19 18 17 21 18 18 12 14 21 27 37 34 34 28 28 34
1850
+ 27 27 28 31 20 18 11 9 9 12 11 11 27 15 15 20 23 26 14 8 8 8 8 14 16
1851
+ 23 19 19 28 31 31 31 25 18 11 11 18 10 10 10 10 11 12 16 16 21 23 12
1852
+ 12 13 23 13 13 19 11 10 10 10 10 10 18 21 28 16 19 29 32 19 19 8 8 8
1853
+ 10 10 18 18 22 21 16 18 22 21 11 11 14 14 15 14 18 7 7 7 7 7 7 14 18
1854
+ 10 10 9 9 14 15 15 23 20 22 19 19 21 16 11 11 9 8 8 9 9 18 14 11 9 9
1855
+ 15 8 9 10 8 9 8 8 8 14 14 25 27 16 9 8 14 8 9 17 13 18 15 8 8 7 7 7 7
1856
+ 6 6 6 6 6 4 4 4 8 9 10 17 11 15 12 17 9 9 10 7 7 7 10 11 10 7 7 7 6 6
1857
+ 8 6 6 8 4
1858
+
1859
+
1860
+ Sequence : 22ak81h10.s1t
1861
+ Is_read
1862
+ Padded
1863
+ Staden_id 5
1864
+ Clipping QUAL 36 418
1865
+ Align_to_SCF 1 523 1 523
1866
+ SCF_File 22ak81h10.s1tSCF
1867
+ Primer Universal_primer
1868
+ Strand Forward
1869
+ Dye Dye_terminator
1870
+ Template 22ak81h10
1871
+ Clone bK216E10
1872
+ Sequencing_vector "m13mp18"
1873
+ Seq_vec SVEC 1 35 "M13mp1"
1874
+ Tag ALUS 36 216
1875
+ Tag DONE 103 103 "AUTO-EDIT: replaced N by g at 103 (terminated, isolated, strong)"
1876
+ Tag DONE 146 146 "AUTO-EDIT: replaced T by g at 146 (terminated, isolated, strong)"
1877
+ Tag DONE 152 152 "AUTO-EDIT: replaced A by g at 152 (terminated, isolated, strong)"
1878
+ Tag DONE 194 194 "AUTO-EDIT: replaced G by c at 194 (all_stranded, isolated, strong)"
1879
+ Tag DONE 219 219 "AUTO-EDIT: replaced G by a at 219 (all_stranded, compound, strong)"
1880
+ Tag DONE 220 220 "AUTO-EDIT: replaced C by a at 220 (all_stranded, compound, strong)"
1881
+ Tag DONE 223 223 "AUTO-EDIT: replaced G by a at 223 (all_stranded, isolated, strong)"
1882
+ Tag DONE 232 232 "AUTO-EDIT: replaced C by g at 232 (all_stranded, isolated, strong)"
1883
+ Tag DONE 237 237 "AUTO-EDIT: replaced G by a at 237 (all_stranded, isolated, strong)"
1884
+ Tag DONE 280 280 "AUTO-EDIT: replaced G by a at 280 (terminated, isolated, strong)"
1885
+ Tag DONE 327 327 "AUTO-EDIT: replaced G by a at 327 (terminated, isolated, strong)"
1886
+ Tag DONE 375 375 "AUTO-EDIT: replaced T by a at 375 (terminated, compound, strong)"
1887
+ Tag DONE 376 376 "AUTO-EDIT: replaced C by a at 376 (terminated, compound, strong)"
1888
+ Tag DONE 407 407 "AUTO-EDIT: replaced C by t at 407 (terminated, isolated, strong)"
1889
+
1890
+ DNA : 22ak66c6.s1t
1891
+ nCGTGGCTGCCTGCAGGTCGACTCTAGAGGATCCCCACCGTCTCTACTAAAATAAAAAGA
1892
+ AATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAGCCACTTGGGAAGCTGAGGCAGG
1893
+ AGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCGACTGCACTC
1894
+ CAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGACAAGACAACT
1895
+ AGTACAGTACTTACAGGGTTATTATGATGATTAAATGAGAGAATAGCTGTGAGGTGATTG
1896
+ ATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATACGGGTTGAGTGTTTCTAATC
1897
+ TGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAATG
1898
+ TTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGGGTTGGGAATATTC
1899
+ AACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAATCTGAAACATtTC
1900
+ TGGTCCTAAGCAAGCATnTTGCAAAGGGATACGCAACCTGTAGTACGTCCTTTATCATTG
1901
+ TnTCAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAnCAnATTCGATTGTA
1902
+ TTATT
1903
+
1904
+ BaseQuality : 22ak66c6.s1t
1905
+ 4 4 8 4 6 10 13 21 24 25 33 33 33 30 27 21 15 27 19 30 30 33 33 30 21
1906
+ 21 10 9 17 11 27 27 37 38 33 35 35 35 35 44 45 45 38 37 37 45 45 45 43
1907
+ 43 43 45 45 45 45 45 45 45 21 21 23 30 30 34 37 37 38 45 32 45 37 41
1908
+ 37 45 30 45 45 34 34 34 32 29 22 32 32 45 45 45 33 28 28 37 37 34 34
1909
+ 34 35 34 34 34 34 34 34 37 37 40 41 37 34 37 37 37 32 24 22 27 29 25
1910
+ 27 20 21 21 21 27 45 41 40 40 40 42 34 34 37 41 45 51 45 45 37 30 37
1911
+ 41 41 37 36 28 30 30 30 22 33 33 35 33 33 41 51 51 45 39 39 39 30 28
1912
+ 33 34 34 39 34 41 34 34 41 33 33 39 39 39 33 33 33 30 33 33 33 30 29
1913
+ 19 19 24 25 32 33 45 36 36 36 36 41 41 30 30 30 37 37 43 51 51 51 51
1914
+ 45 37 37 30 37 37 37 51 51 51 51 51 37 37 28 28 10 10 10 13 10 10 10 9
1915
+ 9 9 21 21 28 37 37 37 33 33 33 33 33 33 34 34 34 33 33 33 33 21 25 25
1916
+ 22 22 29 29 33 33 33 33 31 31 27 28 17 23 23 28 26 24 32 10 10 10 15
1917
+ 15 26 32 32 37 45 45 32 45 33 26 24 32 37 37 35 34 37 35 37 34 37 37
1918
+ 40 37 37 45 45 38 38 45 45 40 37 37 37 34 30 30 28 28 28 30 37 37 34
1919
+ 34 34 34 34 33 31 31 25 21 19 22 21 25 30 32 32 30 22 22 25 28 24 25
1920
+ 26 17 17 23 27 27 33 31 31 34 38 38 38 38 34 26 17 15 8 8 11 16 25 31
1921
+ 32 33 29 26 32 29 33 34 34 34 36 33 34 34 31 27 31 34 38 45 45 36 37
1922
+ 37 37 36 36 38 34 32 28 25 22 22 21 17 18 29 29 23 23 22 27 25 20 18
1923
+ 11 11 15 15 16 23 21 17 19 24 24 24 31 31 31 33 33 33 31 33 33 29 29
1924
+ 29 29 29 31 31 31 23 24 19 12 10 16 10 10 20 10 10 13 15 30 23 29 23
1925
+ 28 18 10 10 16 21 18 19 19 24 24 12 11 9 9 10 21 23 31 31 33 28 28 14
1926
+ 17 17 28 21 30 24 30 28 22 26 23 19 10 10 12 23 22 23 19 12 10 9 9 10
1927
+ 19 24 29 30 34 34 34 34 34 29 25 25 32 26 31 31 32 20 17 15 12 12 4 4
1928
+ 4 17 23 33 30 24 18 13 18 16 12 16 8 9 9 19 15 15 4 4 4 7 11 6 6 6 7 8
1929
+ 9 17 12 13 19 23 25 13 13 13 19 21 29 32 30 26 20 20 12 9 9 8 9 8 8 15
1930
+ 24 17 15 8 8 9 17 16 4 4 4 8 8 13 15 23 14 9 8 8 10 10 17 23 21 17 12
1931
+ 13 22 20 19 15 11 11 9 8 8 8 8 8 8 10 9 9 7 8 8 10 12 10 10 12 4 4 4 4
1932
+ 4 4 10 10 10 10 10 9 8 8 7 8 9 11 9 9 4
1933
+
1934
+
1935
+ Sequence : 22ak66c6.s1t
1936
+ Is_read
1937
+ Padded
1938
+ Staden_id 18
1939
+ Clipping QUAL 37 548
1940
+ Align_to_SCF 1 665 1 665
1941
+ SCF_File 22ak66c6.s1tSCF
1942
+ Primer Universal_primer
1943
+ Strand Forward
1944
+ Dye Dye_terminator
1945
+ Template 22ak66c6
1946
+ Clone bK216E10
1947
+ Sequencing_vector "m13mp18"
1948
+ Seq_vec SVEC 1 36 "M13mp"
1949
+ Tag ALUS 38 221
1950
+ Tag DONE 538 538 "AUTO-EDIT: replaced N by t at 538 (terminated, isolated, strong)"
1951
+
1952
+ DNA : 22ak93c1.s1lt
1953
+ TTTCTTTGGGGGCAATCTTGATGCTAGCGTCGTTCAAGATGATCCACACCCAGCTCACTG
1954
+ TTTTATTCTCTCCAATAATCCTAAGCAAGCTCTCAAAATATAAATATGCTTTTTAATAAT
1955
+ ACAATCGAATTTGCTATACCACCTCAGAATCTGTACCACAATATATTAACTACTTAAAAC
1956
+ AATGATAAAGAACGTACTACAGGTTGCGTATCCCTTTGCAAAATGCTTGCTTAGGACCAG
1957
+ AAATGTTTCAGATTTCAGATTTTTTTTGGAATATTTGCATTTTATAGTACTTACTGGTTG
1958
+ AATATTCCCAACCCAAAAATCTAACAACCCATAATGCTCCAATAAGCACTTCCTTTGAAC
1959
+ ATTAGGTCGGTGCTCAAAAAAGTTTCAGACTGTAGAGCATTTCTAATTTTGGATTTTCAG
1960
+ ATTAGAAACACTCAACCCGTATAATAAAATGATAGTTTGTATTAAGCACAGCACTATATC
1961
+ AATCACCTCACAGCTATTCTCTCATTTAATCATCATAATAACCCTGTAAGTACTGTACTA
1962
+ gTTGTCTTGTCTCTGCTTTTTTTTTTTCgAgACAGAGTCTCACACTATTGGTTGGGCTGG
1963
+ AATGCAnTCGCGTGATCTCGGCTCACTGGCAACCTCCACCTCCCGGGG
1964
+
1965
+ BaseQuality : 22ak93c1.s1lt
1966
+ 4 4 4 4 4 4 4 4 9 6 4 8 15 11 4 6 4 12 8 9 10 12 11 8 8 6 7 8 14 14 9
1967
+ 4 4 8 8 8 8 8 4 6 8 11 9 10 8 9 9 11 10 9 9 9 9 9 9 16 16 32 23 29 21
1968
+ 27 27 22 22 20 28 32 37 37 34 31 30 28 28 24 29 29 34 34 38 32 32 18
1969
+ 22 18 22 22 28 29 37 37 37 37 35 35 34 34 35 34 37 37 37 37 37 37 34
1970
+ 37 37 37 37 32 32 28 28 31 30 30 30 23 23 23 28 27 21 32 13 13 16 24
1971
+ 32 34 31 30 28 28 28 28 28 34 34 34 34 33 25 16 9 9 10 23 32 33 41 37
1972
+ 34 28 30 30 30 28 28 22 27 25 32 25 30 27 22 24 22 22 28 25 24 30 30
1973
+ 30 30 30 30 24 28 13 10 10 23 20 18 9 9 9 18 23 30 30 30 28 24 19 25
1974
+ 11 11 12 27 31 34 30 30 30 30 37 45 51 41 39 28 28 28 25 25 24 30 34
1975
+ 39 39 39 39 39 39 36 31 13 10 9 10 10 23 23 9 9 13 23 31 31 14 14 31
1976
+ 31 13 9 9 9 23 23 23 13 9 9 9 31 31 41 51 36 31 14 13 31 19 30 31 31
1977
+ 22 34 25 33 33 33 30 24 29 30 18 24 24 11 11 24 24 33 39 34 34 30 30
1978
+ 34 22 22 14 19 18 23 23 17 31 24 37 33 33 33 33 33 28 34 18 18 27 34
1979
+ 34 38 27 20 20 24 24 30 30 39 34 34 30 22 22 24 33 33 33 33 28 24 24
1980
+ 22 30 22 24 24 33 33 33 39 51 51 41 32 27 17 25 17 31 31 34 19 15 10
1981
+ 10 31 14 27 21 30 30 24 24 30 34 39 39 41 36 34 30 30 30 34 31 16 9 9
1982
+ 10 20 19 16 11 9 9 9 13 23 22 28 28 37 33 33 33 28 32 18 13 10 20 18
1983
+ 10 32 21 31 23 14 9 9 9 16 11 9 9 10 20 25 33 30 30 28 32 28 28 37 35
1984
+ 35 34 34 34 34 34 37 40 37 33 32 25 12 10 10 16 16 28 32 32 34 30 28
1985
+ 28 29 31 33 32 28 30 30 25 21 16 19 19 19 27 19 14 14 22 22 28 25 29
1986
+ 29 29 30 30 23 23 21 18 14 21 17 24 19 32 32 37 37 31 30 30 28 31 30
1987
+ 34 34 28 28 28 28 31 31 30 28 28 28 30 30 25 32 10 10 10 23 23 27 17
1988
+ 17 22 17 23 23 23 24 20 17 4 4 4 14 16 24 22 19 18 13 19 9 12 14 25 25
1989
+ 33 32 29 40 38 49 49 49 34 34 17 14 8 7 7 9 9 10 8 7 7 9 10 15 17 18
1990
+ 22 24 18 18 18 22 20 24 24 28 18 19 18 19 21 24 25 14 12 8 8 9 14 12 4
1991
+ 4 4 17 17 17 14 9 9 9 12 21 21 18 12 10 13 10 11 9 12 12 8 7 8 8 9 10
1992
+ 9 15 10 8 9 10 10 21 21 28 27 18 14 7 7
1993
+
1994
+
1995
+ Sequence : 22ak93c1.s1lt
1996
+ Is_read
1997
+ Padded
1998
+ Staden_id 1
1999
+ Clipping QUAL 56 598
2000
+ Align_to_SCF 1 648 1 648
2001
+ SCF_File 22ak93c1.s1ltSCF
2002
+ Primer Universal_primer
2003
+ Strand Forward
2004
+ Dye Dye_primer
2005
+ Template 22ak93c1
2006
+ Clone bK216E10
2007
+ Sequencing_vector "m13mp18"
2008
+ Seq_vec SVEC 1 47 "M13mp18"
2009
+ Tag DONE 541 541 "AUTO-EDIT: replaced N by g at 541 (all_stranded, isolated, strong)"
2010
+ Tag ALUS 559 647
2011
+ Tag DONE 569 569 "AUTO-EDIT: replaced A by g at 569 (all_stranded, isolated, strong)"
2012
+ Tag DONE 571 571 "AUTO-EDIT: replaced A by g at 571 (all_stranded, isolated, strong)"
2013
+
2014
+ DNA : 22ak64e2.s1t
2015
+ GTAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTGCAAAATGCTTGCTTA
2016
+ GGACCAGAAATGTTTCAGATTTCAGATTTTTTTTGGAATATTTGCATTTTATAGTACTTA
2017
+ CTGGTTGAATATTCCCAACCCAAAAATCTAACAACCCATAATGCTCCAATAAGCACTTCC
2018
+ TTTGAACATTAGGTCGGTGCTCAAAAAAGTTTCAGACTGTAGAGCATTTCTAATTTTGGA
2019
+ TTTTCAGATTAGAAACACTCAACCCGTATAATAAAATGATAGTTTGTATTAAGCACAGCA
2020
+ CTATATCAATCACCTCACAGCTATTCTCTCATTTAATCATCATAATAACCCTGTAAGTAC
2021
+ TGTACTAGTTGTCTTGTCTCTGCTTTTTTTTTTTCGAGACAGAGTCTCACACTATTGGTT
2022
+ GGACTGGAGTGCAATTCnCCTGAATTCGGCTCACGGCAACTTCAACCTCCCGGGTTCAAG
2023
+ TGAATCTCCTC
2024
+
2025
+ BaseQuality : 22ak64e2.s1t
2026
+ 4 4 8 10 12 15 18 18 28 30 30 30 30 26 37 34 30 30 22 22 20 23 16 11
2027
+ 11 18 23 23 23 28 9 9 10 9 21 12 32 34 45 45 45 45 38 30 30 30 30 30
2028
+ 28 34 30 33 33 33 33 33 43 45 45 32 18 10 11 11 25 23 10 10 22 32 38
2029
+ 37 27 27 27 23 20 9 9 10 23 25 25 21 9 9 10 29 32 45 45 45 45 45 45 37
2030
+ 37 37 38 45 37 41 28 29 28 28 28 28 34 34 33 33 33 28 28 34 33 34 34
2031
+ 34 34 34 34 45 37 37 29 37 37 45 45 45 45 42 37 34 35 35 35 34 34 34
2032
+ 34 34 37 37 37 45 45 35 35 35 35 35 35 35 37 37 37 37 45 37 42 42 33
2033
+ 30 30 33 33 33 40 34 34 34 34 34 34 40 40 40 37 37 37 34 34 34 34 34
2034
+ 31 31 27 28 19 21 19 28 28 30 32 34 34 31 31 31 33 39 27 20 7 7 8 8 14
2035
+ 15 11 11 17 28 23 28 17 9 9 11 23 27 33 33 40 40 40 40 40 40 40 37 32
2036
+ 32 31 31 28 37 30 33 25 21 13 13 14 26 22 11 11 21 21 30 33 31 31 33
2037
+ 37 37 37 45 49 45 40 40 40 40 40 45 40 40 40 40 40 31 29 29 32 27 37
2038
+ 37 45 45 45 40 37 37 37 37 37 33 34 33 33 30 30 34 34 36 36 36 30 30
2039
+ 28 28 28 27 38 38 36 31 33 32 27 20 20 20 28 32 33 24 24 29 36 34 30
2040
+ 33 30 26 26 26 30 30 26 37 36 34 33 30 31 31 28 27 27 25 24 29 33 27
2041
+ 27 31 31 31 31 33 36 38 38 32 32 21 13 13 16 19 19 24 25 33 30 33 33
2042
+ 33 38 39 40 44 40 40 36 33 37 40 40 40 40 49 40 36 33 31 26 23 23 21
2043
+ 18 20 14 12 11 14 17 25 31 33 33 33 36 30 30 30 30 30 28 28 20 15 14 4
2044
+ 4 4 12 9 9 4 4 4 7 8 7 7 7 7 4 4 4 7 8 10 7 6 9 9 9 7 7 11 17 19 9 8 7
2045
+ 7 8 7 8 9 7 6 10 6 7 6 6 7 7 8 8 8 12 13 8 8 8 8 10 10 9 8 7 6 6 8 13
2046
+ 7 7 7 7
2047
+
2048
+
2049
+ Sequence : 22ak64e2.s1t
2050
+ Is_read
2051
+ Padded
2052
+ Staden_id 14
2053
+ Clipping QUAL 41 420
2054
+ Align_to_SCF 1 491 1 491
2055
+ SCF_File 22ak64e2.s1tSCF
2056
+ Primer Universal_primer
2057
+ Strand Forward
2058
+ Dye Dye_terminator
2059
+ Template 22ak64e2
2060
+ Clone bK216E10
2061
+ Sequencing_vector "m13mp18"
2062
+ Seq_vec SVEC 1 40 "M13mp"
2063
+
2064
+ DNA : 22ak70f10.s1t
2065
+ TTGGCTGCCTGCAGGTCGACTCTAGAGGATCCCCCAGGGTTATTATGATGATTAAATGAG
2066
+ AGAATAGCTGTGAGGTGATTGATATAGTGCTGTGCTTAATACAAACTATCATTTTATTAT
2067
+ ACGGGTTGAGTGTTTCTAATCTGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACT
2068
+ TTTTTGAGCACCGACCTAATGTTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAG
2069
+ ATTTTTGGGTTGGGAATATTCAACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAA
2070
+ ATCTGAAATCTGAAACATTTCTGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCT
2071
+ GTAGTACGTTCTTTATCATTGTTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGT
2072
+ GGTATAGCAAATTCGATTGTATTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAG
2073
+ GATTATTGGAGAGAATAAAACAGTGAAGCTTTGGTGTTATGAGGGAATnTTAGATAGAAA
2074
+ AGTGCAGTnTTTCAGTTCATGCTCTnTCATTTnTTACTCCCTCCGGTTAAAGCTGAAGCT
2075
+ CAACAAAGATATAGTGATCTCTGTGGGCATTATAATCCTGTCCC
2076
+
2077
+ BaseQuality : 22ak70f10.s1t
2078
+ 4 4 6 8 8 9 19 23 33 33 34 33 27 21 18 27 18 27 27 34 34 31 27 23 13
2079
+ 11 19 16 31 31 37 45 45 34 34 31 31 22 31 25 31 37 45 45 45 45 37 37
2080
+ 37 37 37 37 45 37 37 37 45 38 29 27 27 32 32 37 45 33 37 37 45 37 45
2081
+ 45 37 37 37 40 40 40 37 45 37 37 34 33 33 34 34 34 37 33 40 37 37 37
2082
+ 33 33 33 33 33 33 33 33 33 32 33 41 41 35 35 35 40 40 36 36 36 34 33
2083
+ 33 37 37 37 37 37 41 45 30 30 41 41 37 28 45 18 18 29 35 37 37 37 37
2084
+ 41 41 51 51 51 51 51 33 33 33 33 33 33 45 45 45 41 39 39 51 51 41 45
2085
+ 45 45 45 35 33 33 33 33 33 40 51 51 51 51 51 51 51 51 51 43 45 39 34
2086
+ 30 30 33 33 33 33 45 45 38 38 38 38 33 30 29 14 15 27 27 30 33 51 51
2087
+ 41 41 37 34 37 37 37 40 40 40 37 45 51 33 33 33 33 33 33 37 37 31 45
2088
+ 33 25 22 27 21 28 33 28 28 33 41 37 45 41 33 45 33 22 22 33 33 34 44
2089
+ 44 45 45 33 33 33 33 33 30 34 34 34 34 37 37 29 34 37 33 33 33 33 33
2090
+ 33 34 40 33 33 33 30 30 30 37 37 37 30 30 33 30 33 33 49 49 45 45 45
2091
+ 45 45 45 44 38 38 38 44 38 45 45 49 40 40 35 35 35 35 38 37 40 37 37
2092
+ 34 34 34 34 34 34 34 36 36 36 34 36 33 31 31 36 33 28 28 27 23 19 17
2093
+ 17 23 23 23 33 27 28 28 28 30 33 40 40 31 31 17 20 20 28 32 25 17 17
2094
+ 32 27 37 37 36 33 33 33 33 32 36 34 28 30 32 31 31 14 18 10 9 9 19 21
2095
+ 30 31 38 38 38 37 37 31 34 34 36 31 30 31 31 31 31 33 33 33 39 30 25
2096
+ 24 24 29 33 33 34 33 33 23 23 12 23 23 30 33 33 37 37 37 38 40 37 34
2097
+ 33 37 36 33 33 33 33 33 33 36 28 28 22 24 24 24 21 29 33 33 33 34 33
2098
+ 33 33 33 33 33 33 33 33 27 28 28 31 31 23 27 19 15 11 20 21 33 33 31
2099
+ 31 31 31 31 26 28 24 24 24 29 24 29 29 33 27 31 27 30 36 29 18 20 12
2100
+ 17 9 9 9 19 19 15 8 8 13 15 14 8 9 9 9 10 12 4 4 4 9 10 11 12 24 26 18
2101
+ 13 13 11 11 13 10 16 14 13 12 4 4 4 17 11 7 7 8 7 8 8 8 20 20 26 16 14
2102
+ 4 4 4 8 8 10 18 4 4 4 8 8 21 11 13 10 8 10 6 6 6 6 6 8 9 12 14 17 11
2103
+ 14 14 8 8 8 7 9 7 8 8 12 9 9 8 8 7 10 10 12 10 9 8 16 9 8 8 8 7 6 6 8
2104
+ 6 6 6 6 6 6 6 9 12 7 7 8 8 7 7 7 12 8 7 7
2105
+
2106
+
2107
+ Sequence : 22ak70f10.s1t
2108
+ Is_read
2109
+ Padded
2110
+ Staden_id 2
2111
+ Clipping QUAL 35 508
2112
+ Align_to_SCF 1 644 1 644
2113
+ SCF_File 22ak70f10.s1tSCF
2114
+ Primer Universal_primer
2115
+ Strand Forward
2116
+ Dye Dye_terminator
2117
+ Template 22ak70f10
2118
+ Clone bK216E10
2119
+ Sequencing_vector "m13mp18"
2120
+ Seq_vec SVEC 1 34 "M13mp1"
2121
+ Seq_vec SVEC 584 644 "M13mp1"
2122
+
2123
+ DNA : 22ak92c4.s1t
2124
+ nGGGCGCTGCCTGCAGGTCGACTCTAnAGGATCCCCATTATACGGGTTGAGTGTTTCTAA
2125
+ TCTGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAA
2126
+ TGTTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGGGTTGGGAATAT
2127
+ TCAACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAATCTGAAACATT
2128
+ TCTGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCTGTAGTACGTTCTTTATCAT
2129
+ TGTTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAGCAAATTCGATTG
2130
+ TATTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAGGATTATTGGAGAGAATAAA
2131
+ ACAGTGAAGCTTTGGTGTTATGAGGGAATTTTAGATAGAAAAGTGCAGTTTTTCAGTTCA
2132
+ TGCTCTTTCATTTC
2133
+
2134
+ BaseQuality : 22ak92c4.s1t
2135
+ 4 4 6 4 6 8 11 14 18 26 32 34 36 34 23 25 10 23 9 23 23 34 29 23 22 4
2136
+ 4 4 16 14 25 24 29 33 30 33 33 33 33 40 37 40 45 28 28 25 15 18 32 32
2137
+ 17 14 28 13 13 38 38 40 40 37 37 37 37 38 45 45 45 38 33 32 33 33 33
2138
+ 33 38 38 38 45 32 30 45 32 34 34 34 34 40 33 33 32 32 28 28 38 45 45
2139
+ 49 45 45 45 45 40 37 41 45 45 40 30 30 33 33 33 33 40 40 45 45 45 45
2140
+ 32 32 28 11 12 27 25 33 33 45 41 33 32 14 14 28 33 37 37 37 37 37 45
2141
+ 45 37 37 37 37 37 37 37 37 28 33 32 21 14 32 20 24 32 21 21 32 36 41
2142
+ 41 45 32 32 28 13 13 33 33 45 51 51 41 37 32 32 32 32 32 32 34 34 30
2143
+ 30 31 32 12 12 27 31 30 35 35 35 33 39 41 28 28 28 28 28 28 41 41 41
2144
+ 30 32 30 30 30 32 51 51 51 51 45 45 45 45 45 45 45 45 51 45 45 45 45
2145
+ 33 33 33 33 33 33 51 51 51 51 51 41 41 45 45 45 39 39 39 39 39 39 41
2146
+ 41 41 41 40 33 33 33 33 33 33 41 45 41 41 41 41 33 33 33 33 33 33 51
2147
+ 51 45 34 22 22 20 28 30 17 15 15 45 30 33 33 33 33 33 33 33 33 37 37
2148
+ 32 33 33 25 25 9 10 9 14 14 33 33 34 34 41 40 40 40 40 37 40 37 37 34
2149
+ 33 33 29 24 22 32 32 45 45 40 27 27 14 32 32 45 38 34 45 19 21 10 21
2150
+ 21 30 37 37 34 34 37 38 38 37 37 37 37 37 35 34 36 36 40 40 49 34 34
2151
+ 34 34 37 37 38 37 33 30 30 30 30 30 40 40 34 34 34 27 27 31 31 33 35
2152
+ 33 30 30 21 21 12 30 28 36 40 36 36 36 33 33 26 26 21 19 20 29 30 36
2153
+ 34 34 33 31 28 28 32 35 32 35 26 30 25 26 26 25 21 19 12 11 19 21 23
2154
+ 21 11 13 17 17 24 29 27 29 29 28 31 20 25 18 16 12 14 23 21 11 11 16
2155
+ 14 10 21 23 33 33 34 31 31 22 22 14 21 11 16 18 31 40 40 36 36 24 27
2156
+ 19 19 13 16 10 8
2157
+
2158
+ Sequence : 22ak92c4.s1t
2159
+ Is_read
2160
+ Padded
2161
+ Staden_id 16
2162
+ Clipping QUAL 37 490
2163
+ Align_to_SCF 1 494 1 494
2164
+ SCF_File 22ak92c4.s1tSCF
2165
+ Primer Universal_primer
2166
+ Strand Forward
2167
+ Dye Dye_terminator
2168
+ Template 22ak92c4
2169
+ Clone bK216E10
2170
+ Sequencing_vector "m13mp18"
2171
+ Seq_vec SVEC 1 36 "M13mp1"
2172
+
2173
+ DNA : 22ap08f1.s1t
2174
+ GAACTATCATnnnATATACGGGTTGAGTGTnTCTAATCTGAAAATCCAAAATTAGAAATG
2175
+ CTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAATGTTCAAAGGAAGTGCTTATTGGA
2176
+ GCATTATGGGTTGTTAgATTTTTGGGTtGGGAATATTCAACCATTAAGCTACACACAATG
2177
+ CAAATATCCCACAAACAATCTGAAATCTGAAACACTTCTGGTCCAAGCAAGCATCCGCAA
2178
+ CGGATACGCAACCTGCACACGTTCTTATCATCGTTAACTCTnATATATGTGGACAAATCT
2179
+ GACGTGGTACACACACTCATGTATATCACAACATATTACATTTGAAGCTGCTACGAATAT
2180
+ GGAAGAATnnnnCCTGAACTTGGTGTATGACGAACTTAGATAGACAGTGACTCTTCAGTT
2181
+ CAGCTCnTCATTTnTnnTCCCTCGGTTTTnCTAAnCnCnnCnnAAAAnnnnATCCnGTGG
2182
+ GCATAAAnnCnGGTCCCACCGCCCCCnCCCCACAAAACCACACACTACCGACCTCCCAA
2183
+
2184
+ BaseQuality : 22ap08f1.s1t
2185
+ 7 9 10 13 14 16 21 15 14 4 4 4 4 4 11 11 20 26 28 28 30 30 30 25 25 20
2186
+ 25 23 28 4 4 4 18 18 18 21 24 31 33 34 33 34 34 40 30 31 30 24 24 24
2187
+ 31 27 30 31 30 23 32 32 37 36 38 34 33 24 18 15 10 8 8 18 25 32 33 34
2188
+ 31 31 33 29 21 12 9 7 8 11 13 18 22 25 25 33 33 32 29 22 27 24 25 16 9
2189
+ 9 19 21 20 14 16 10 12 12 11 11 18 23 21 19 19 10 10 10 20 9 9 13 18
2190
+ 18 20 21 18 17 14 16 9 9 9 10 10 10 9 9 21 25 22 18 18 14 20 21 13 13
2191
+ 21 17 26 25 23 20 15 10 12 16 17 15 10 11 8 8 8 8 6 4 4 4 8 10 8 8 6 6
2192
+ 8 13 15 21 21 15 11 9 12 11 7 6 6 6 6 6 6 10 6 6 6 6 10 14 10 10 9 12
2193
+ 9 11 10 14 9 13 10 10 12 9 9 15 17 20 16 17 21 25 10 8 10 10 7 7 10 9
2194
+ 7 8 14 7 7 8 10 8 6 6 6 8 8 10 11 14 8 9 11 19 17 13 9 9 9 8 8 10 9 8
2195
+ 8 8 9 8 11 11 10 8 8 8 8 6 6 8 10 8 7 6 4 8 4 4 4 7 7 11 10 12 9 12 10
2196
+ 10 10 11 6 6 9 9 14 8 7 7 7 7 12 16 11 6 6 6 6 6 6 6 6 6 8 13 9 8 8 15
2197
+ 12 10 9 8 8 8 8 7 9 12 8 6 6 8 4 6 6 7 11 13 13 7 8 9 7 7 12 12 10 9 4
2198
+ 4 4 6 7 7 12 14 7 7 12 6 6 6 7 4 4 4 4 4 4 8 8 14 12 13 16 16 16 10 11
2199
+ 16 14 12 7 6 6 4 4 4 8 7 7 7 12 7 7 8 11 4 4 4 4 4 6 6 6 6 6 6 6 6 6 4
2200
+ 4 8 6 6 12 12 12 9 8 4 4 4 6 11 6 9 4 4 4 4 4 4 9 9 9 15 9 9 6 6 6 8 4
2201
+ 4 4 9 9 4 4 4 4 4 4 4 4 4 4 4 9 9 4 4 4 4 4 4 11 12 4 4 4 8 8 15 9 6 8
2202
+ 9 9 9 4 4 4 4 4 4 7 10 6 8 15 8 8 4 6 14 15 17 18 4 4 4 14 8 6 6 8 8 9
2203
+ 13 9 9 9 7 7 7 7 8 8 6 6 8 6 6 6 8 6 6 8 8 8 8 4
2204
+
2205
+ Sequence : 22ap08f1.s1t
2206
+ Is_read
2207
+ Padded
2208
+ Staden_id 17
2209
+ Clipping QUAL 17 155
2210
+ Align_to_SCF 1 539 1 539
2211
+ SCF_File 22ap08f1.s1tSCF
2212
+ Primer Universal_primer
2213
+ Strand Forward
2214
+ Dye Dye_terminator
2215
+ Template 22ap08f1
2216
+ Clone bK216E10
2217
+ Sequencing_vector "m13mp18"
2218
+ Tag DONE 137 137 "AUTO-EDIT: replaced T by g at 137 (terminated, isolated, strong)"
2219
+ Tag DONE 148 148 "AUTO-EDIT: replaced A by t at 148 (terminated, isolated, strong)"
2220
+
2221
+ DNA : 22ai01c4.s1t
2222
+ GGAACATGCTGCAGGTCGACTCTAGAGGATCCCCGCTCTACAGTCTGAAACTTTTTTGAG
2223
+ CACCGACCTAATGTTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGG
2224
+ GTTGGGAATATTCAACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAA
2225
+ TCTGAAACATTTCTGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCTGTAGTACG
2226
+ TTCTTTATCATTGTTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAGC
2227
+ AAATTCGATTGTATTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAGGATTATTG
2228
+ GAGAGAATAAAACAGTGAAGCTTTGGTGTTATGAGGGAATTTTAGATAGAAAAGTGCAGT
2229
+ TTTTCAGTTCATGCTCTTTCATTTTTTACTCCCTCAGGTTAAAGCT-GAAGCTCAACAAA
2230
+ GATATAGTGATCTCTGTGGGCATTTATAATCTGGTCCAGAAGGCTCTCAAGCCTCCTCCA
2231
+ ATAAAGCTCTATCGGGAAACAAATGAAACAGTGAAAACCAAGAACCGGAACTTTAATACA
2232
+ AGTACAGGCGGTTTGCTTTCTGCTAGCGATACCAAAAGTCTCAGGTAGGTAAAAATGCTn
2233
+ nTGTGTTA
2234
+
2235
+ BaseQuality : 22ai01c4.s1t
2236
+ 4 4 6 8 8 18 18 25 25 30 25 21 27 16 16 27 24 27 31 37 33 33 31 31 21
2237
+ 21 23 25 37 34 37 45 45 45 45 40 40 40 33 33 30 30 30 30 38 45 45 49
2238
+ 45 45 45 45 45 45 45 45 38 38 30 30 30 33 33 30 37 37 40 42 42 45 38
2239
+ 45 35 25 27 33 33 33 33 37 37 37 40 37 37 37 37 42 42 42 40 37 40 45
2240
+ 33 33 33 33 33 33 45 45 41 41 45 37 30 41 32 41 45 37 37 41 41 45 45
2241
+ 41 32 38 37 30 30 30 41 41 51 51 51 51 33 33 35 35 35 29 33 33 33 33
2242
+ 33 45 37 37 37 37 37 40 40 40 40 45 43 35 33 33 33 33 33 43 45 43 32
2243
+ 33 33 28 28 29 51 51 45 51 51 51 51 51 51 51 51 51 51 51 51 51 51 42
2244
+ 42 42 40 39 39 51 51 45 45 45 38 38 37 37 38 33 33 33 33 33 33 37 40
2245
+ 39 39 42 33 33 33 33 33 33 37 37 41 41 41 41 33 33 33 33 33 33 51 45
2246
+ 41 41 39 34 34 29 31 37 34 34 45 37 38 42 33 32 32 32 32 32 37 51 32
2247
+ 32 32 32 32 21 24 12 25 25 33 33 33 33 40 40 40 37 37 41 45 45 45 35
2248
+ 34 34 34 34 34 41 41 45 45 45 41 37 37 41 40 45 37 37 42 33 33 22 33
2249
+ 33 33 37 37 37 37 37 37 37 37 37 36 33 33 33 33 33 33 40 40 45 34 34
2250
+ 34 34 34 34 37 37 40 33 33 33 33 33 36 36 40 34 37 37 37 37 38 38 36
2251
+ 33 33 33 31 31 20 29 27 34 34 34 40 40 34 34 38 38 38 34 34 40 37 40
2252
+ 37 30 30 30 30 30 34 36 40 40 36 36 28 33 32 27 27 18 14 14 18 23 27
2253
+ 34 33 29 29 24 29 29 27 27 28 28 25 25 33 22 24 23 31 28 33 34 31 25
2254
+ 25 25 25 30 31 36 32 27 18 13 13 25 25 17 23 25 31 45 45 45 38 31 31
2255
+ 24 22 20 27 35 34 31 33 33 33 33 36 36 36 38 20 13 10 8 12 11 18 20 23
2256
+ 34 34 30 1 29 22 19 24 22 24 28 31 31 31 27 27 23 28 10 25 20 17 10 25
2257
+ 25 35 35 38 38 36 36 27 27 17 17 11 13 16 19 18 18 18 22 15 11 11 15
2258
+ 27 25 24 24 18 8 9 8 7 7 8 7 8 8 15 17 21 14 10 10 19 17 20 25 24 24
2259
+ 21 16 15 21 21 26 33 33 26 23 19 23 23 28 21 20 15 10 8 7 8 6 8 11 7
2260
+ 10 18 17 12 9 9 7 11 9 7 6 7 6 8 9 9 9 6 10 8 10 7 8 8 7 6 6 8 7 8 8
2261
+ 14 23 24 19 14 14 7 7 7 7 8 8 6 6 7 6 6 6 6 6 9 21 13 12 13 15 15 10
2262
+ 11 17 16 15 8 7 7 9 9 10 10 8 8 8 8 9 8 6 8 8 8 7 7 7 7 7 7 7 7 7 7 7
2263
+ 10 8 7 7 8 11 4 4 4 4 9 6 6 6 6 6
2264
+
2265
+
2266
+ Sequence : 22ai01c4.s1t
2267
+ Is_read
2268
+ Padded
2269
+ Staden_id 6
2270
+ Clipping QUAL 35 553
2271
+ Align_to_SCF 1 466 1 466
2272
+ Align_to_SCF 468 668 467 667
2273
+ SCF_File 22ai01c4.s1tSCF
2274
+ Primer Universal_primer
2275
+ Strand Forward
2276
+ Dye Dye_terminator
2277
+ Template 22ai01c4
2278
+ Clone bK216E10
2279
+ Sequencing_vector "m13mp18"
2280
+ Seq_vec SVEC 1 34 "M13mp18"
2281
+
2282
+ DNA : 22ak93c1.s1t
2283
+ nGGTGAAGCTTGCATGCCTGCAGGTCGACTCTAAAGGATCCCCACAcCAAAGCTTCACTG
2284
+ TTTTATTCTCTCCAATAATCCTAAgCAAGCTCTCAAAATATAAATATGCTTTtTAATAAT
2285
+ ACAATCGAATTTGCTATACCACCTCAGAATCTGTACCACAATATATTAACTACTTAAAAC
2286
+ AATGATAAAGAACGTACTACAGGTTGCGTATCCCTTTGCAAAATGCTTGCTTAgGACCAG
2287
+ AAATGTtTCAGATTTCAGATTTTTTTTGGAATATTTGCATTTTATAGTACTTACTGGTTG
2288
+ AATATTCCCAACCCAAAaATCTAACAACCCATAATGCTCCAATAAGCACTTCCTTTGAAC
2289
+ ATTATGTCGGTGCTCAAAAAAGTTTCAGACTGTAAAGCATTTCCTAATTTTGGATTTCCA
2290
+ GAATAGAAACACTCAACCCGTATAATAAAATGATAATCTGTATTAAGCACAGCACTATAT
2291
+ CAAGCACCTCACAGCTATTCTCTCATTTAATCATCATAATAACCCTGTAAGTACTGTACA
2292
+ AGTGGTCTGGTCCCTGCTTTTATTTCTCCAAGAACGACTCTCACACTATTGGTTCGCCTG
2293
+ GAGTGCnTCGCGTGATCCCCGCTCATGGCACCTCCACTCCCGGGTCCAAGCAATTCTCTT
2294
+ GCTCAACTTCCAAGTGGTGGGATTAGGGCCCTGCCATAACCCACTTATTTCTTTTATTTT
2295
+ AATAAAACGTTTCCCCATTTTGGCAGTGGGTTTTACCCTGACCCGTATTGCCCTTCCGGC
2296
+ CCACGATTTGGGGGAACCAGTTGGGCCCCCCTTTGGGCCTCTCCTGTTCCGAGC
2297
+
2298
+ BaseQuality : 22ak93c1.s1t
2299
+ 4 4 4 4 8 8 12 20 25 31 33 33 30 30 30 31 31 35 35 34 27 23 12 11 25
2300
+ 11 14 14 28 32 20 14 6 6 6 6 8 16 16 25 28 15 15 13 17 9 10 9 16 16 18
2301
+ 18 18 25 27 30 26 20 21 19 10 10 14 23 25 30 30 37 37 37 34 31 28 30
2302
+ 22 18 29 18 22 18 15 11 21 12 15 15 22 24 28 37 37 37 37 32 27 10 10
2303
+ 13 18 20 25 25 23 23 24 32 33 40 40 38 32 18 18 18 27 27 28 37 37 21
2304
+ 21 15 15 12 22 32 23 23 30 30 45 45 37 37 32 32 23 22 22 22 27 28 31
2305
+ 26 23 12 12 18 32 38 37 37 37 37 34 34 30 30 30 30 30 30 34 34 37 37
2306
+ 37 34 34 31 31 31 28 27 24 22 22 22 30 30 33 30 30 32 26 24 15 15 9 7
2307
+ 7 14 10 10 9 14 11 12 10 11 6 7 9 8 17 24 26 28 26 28 28 35 34 34 30
2308
+ 26 26 26 28 28 31 31 33 33 31 23 20 15 13 15 18 14 6 6 8 13 13 19 9 7
2309
+ 7 16 10 10 15 10 8 8 12 10 9 9 18 13 14 17 11 7 7 13 21 23 28 21 20 19
2310
+ 19 22 25 27 31 32 32 28 28 25 25 25 25 25 25 31 30 19 19 13 13 13 27
2311
+ 28 33 33 33 33 33 36 33 31 24 21 14 15 18 24 27 31 25 28 30 30 28 23
2312
+ 23 21 17 14 8 9 9 25 25 33 25 25 28 30 30 30 22 21 20 20 22 25 28 34
2313
+ 28 21 16 15 10 14 17 25 25 12 12 12 12 12 20 10 14 16 21 17 13 11 9 12
2314
+ 11 7 6 6 6 8 14 8 8 6 6 11 23 21 28 28 31 31 34 23 10 7 7 7 7 7 9 8 8
2315
+ 14 16 20 20 16 10 7 7 7 14 7 9 14 10 9 14 9 19 22 27 25 13 13 18 14 17
2316
+ 23 16 9 8 9 7 7 7 7 9 8 8 13 16 21 19 15 15 10 10 15 11 18 25 23 21 21
2317
+ 21 32 25 28 25 28 17 20 10 7 7 11 6 7 6 6 7 9 8 6 6 6 6 6 8 7 7 9 13
2318
+ 11 13 13 20 21 18 15 10 10 9 9 8 8 8 9 9 13 16 16 14 14 14 25 10 11 6
2319
+ 6 6 6 7 10 14 9 13 20 8 7 7 8 11 10 13 12 12 13 16 14 15 15 13 13 10
2320
+ 12 7 8 9 14 11 12 12 9 10 8 8 8 10 20 12 9 9 12 6 6 6 6 8 9 6 6 6 6 7
2321
+ 6 6 8 16 18 18 9 6 6 6 13 7 7 7 8 9 9 7 7 7 7 7 6 6 6 6 6 6 6 6 7 8 8
2322
+ 10 9 8 8 6 7 8 4 4 4 4 4 10 7 7 9 9 8 8 4 4 4 4 4 4 8 4 9 9 12 9 8 6 6
2323
+ 6 9 11 7 7 7 7 7 9 11 11 9 6 6 6 10 8 8 7 10 10 14 25 16 10 7 7 7 7 10
2324
+ 10 8 8 7 7 7 8 6 6 6 6 8 11 12 7 6 13 6 7 7 7 8 7 7 7 8 8 10 8 6 6 6 4
2325
+ 4 8 8 10 13 22 24 24 20 14 8 6 6 6 6 6 10 15 15 10 9 6 6 10 8 7 7 9 9
2326
+ 13 13 10 11 11 11 8 7 7 6 14 6 7 8 6 6 7 7 7 7 7 7 12 7 8 10 10 13 14
2327
+ 9 8 8 8 11 9 7 7 7 8 8 11 10 12 11 11 10 10 12 12 17 13 9 8 9 6 6 6 6
2328
+ 6 12 8 8 8 8 9 8 8 9 8 7 7 7 9 9 8 8 6 6 8 8 11 12 8 6 8 6 6 6 6 6 7 8
2329
+ 9 11 12 12 6 6 8 7 8 8 13 7 7 7 6 8 8 8 8 8 8 6 6 8 4 6 4 4 4 4
2330
+
2331
+ Sequence : 22ak93c1.s1t
2332
+ Is_read
2333
+ Padded
2334
+ Staden_id 10
2335
+ Clipping QUAL 44 345
2336
+ Align_to_SCF 1 834 1 834
2337
+ SCF_File 22ak93c1.s1tSCF
2338
+ Primer Universal_primer
2339
+ Strand Forward
2340
+ Dye Dye_terminator
2341
+ Template 22ak93c1
2342
+ Clone bK216E10
2343
+ Sequencing_vector "m13mp18"
2344
+ Seq_vec SVEC 1 43 "M13mp1"
2345
+ Tag DONE 47 47 "AUTO-EDIT: replaced T by c at 47 (terminator, isolated, strong)"
2346
+ Tag DONE 85 85 "AUTO-EDIT: replaced C by g at 85 (terminated, isolated, strong)"
2347
+ Tag DONE 113 113 "AUTO-EDIT: replaced A by t at 113 (terminated, isolated, strong)"
2348
+ Tag DONE 234 234 "AUTO-EDIT: replaced C by g at 234 (terminated, isolated, strong)"
2349
+ Tag DONE 247 247 "AUTO-EDIT: replaced C by t at 247 (terminated, isolated, strong)"
2350
+ Tag DONE 318 318 "AUTO-EDIT: replaced G by a at 318 (terminated, isolated, strong)"
2351
+
2352
+ DNA : 22ak65e10.s1t
2353
+ TGAGGGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAAAATAAATAAATAA
2354
+ ATAAATTAGCTGGGTGTGATGTCACATGCCTGTGATCCCAGCTACTTGGGAGGCTGAGAC
2355
+ AGAAGGATCGCTTGAGTCCAGGAGGTCAAGGCTGTAGCGAGTCATGTTCGCCCCACTGCA
2356
+ CTCCAGCCTGGGCGACACAATGAGACCCTGTCTCAAAAACAACAACAACAAAAGGCATCT
2357
+ CTACCTACCTGAGACCTCTTGGTATCGCTAGGCAGAAGCAAACCGCCTGTACTTGTATTA
2358
+ AAGGTCCGGGTCTTGGTTTTCACTGGTTCATTTGTTTCCCGATAGAGCTTTATTGGAGGA
2359
+ gGCTTGAGAGCCTTCTGGACCAGATTATaAATGCCCACAGAGATCACTATATCTTTGTTG
2360
+ AGCTTC-AGCTTTAACCTGAGGGAGTAAAAAATGAAAGAGCATGAACTGAAAAACTGCAC
2361
+ TTTTCTATCTAAAATTCCCTCATAACACCAAAGCTTCACTGTTTTATTCTCTCCAATAAT
2362
+ CCTAAGCAAGCTCTCAAAATATAAATATGCTTTTTAATAATACAATCC
2363
+
2364
+ BaseQuality : 22ak65e10.s1t
2365
+ 4 4 8 8 9 9 14 15 31 31 28 28 28 30 30 33 33 33 30 22 20 12 22 12 18
2366
+ 18 30 31 27 23 32 10 10 14 12 32 32 37 45 45 45 45 45 38 34 29 29 29
2367
+ 29 29 29 29 32 32 30 34 34 34 34 35 34 34 32 35 29 27 32 32 32 37 45
2368
+ 32 45 37 37 37 24 24 32 32 19 22 11 21 21 31 31 34 45 45 45 28 30 30
2369
+ 37 40 34 33 33 28 28 28 37 45 41 41 41 41 45 41 27 27 33 37 37 32 27
2370
+ 20 20 20 30 20 20 21 24 17 38 32 30 42 37 37 37 30 28 13 12 18 25 21
2371
+ 24 14 25 25 22 28 17 28 28 18 32 36 36 32 32 21 32 32 31 31 27 27 21
2372
+ 32 32 27 13 13 32 33 38 45 36 34 30 30 21 21 23 23 23 23 30 33 33 37
2373
+ 34 41 36 41 37 37 34 29 29 19 30 30 40 37 37 36 37 25 25 26 26 30 45
2374
+ 41 51 37 33 30 28 28 33 20 18 20 20 20 20 26 20 20 18 21 21 31 31 28
2375
+ 32 14 18 20 29 33 34 45 45 40 40 40 37 37 37 37 37 32 45 30 23 23 32
2376
+ 32 37 34 35 34 34 34 34 37 37 37 37 45 37 37 32 27 23 14 12 12 14 17
2377
+ 19 27 27 28 30 37 45 45 45 45 37 37 37 42 42 40 37 37 34 33 33 40 31
2378
+ 26 28 25 27 31 34 34 34 31 27 27 27 31 27 31 23 23 18 18 18 25 25 31
2379
+ 35 31 30 27 25 27 35 33 32 30 21 21 21 23 23 27 26 21 20 16 16 17 18
2380
+ 30 30 36 36 36 34 34 36 34 26 15 12 6 6 6 12 11 23 23 26 17 17 21 32
2381
+ 35 35 40 33 33 33 38 33 27 23 23 12 12 18 18 19 10 8 8 17 19 34 33 34
2382
+ 31 34 31 31 22 22 19 18 27 27 32 22 25 24 32 32 34 34 34 37 34 30 33
2383
+ 27 27 27 31 32 27 27 18 1 12 12 11 15 15 17 27 28 28 34 34 30 25 18 13
2384
+ 19 15 15 25 21 29 26 34 24 28 25 27 21 20 23 24 24 26 29 30 26 27 22
2385
+ 29 27 33 34 34 36 38 38 38 34 31 30 24 24 24 31 33 35 35 29 33 33 31
2386
+ 31 28 28 29 26 22 14 8 9 9 9 14 9 13 18 21 21 27 23 18 15 21 21 25 33
2387
+ 33 31 31 21 21 24 27 29 25 19 19 19 25 27 27 34 34 34 14 9 7 7 9 18 23
2388
+ 27 23 19 19 23 24 21 18 8 8 9 21 20 31 24 20 13 11 16 24 32 32 25 18
2389
+ 13 13 20 20 26 21 27 21 20 13 17 15 21 12 12 18 15 15 15 15 8 8 7 10 8
2390
+ 4
2391
+
2392
+
2393
+ Sequence : 22ak65e10.s1t
2394
+ Is_read
2395
+ Padded
2396
+ Staden_id 4
2397
+ Clipping QUAL 42 575
2398
+ Align_to_SCF 1 426 1 426
2399
+ Align_to_SCF 428 588 427 587
2400
+ SCF_File 22ak65e10.s1tSCF
2401
+ Primer Universal_primer
2402
+ Strand Forward
2403
+ Dye Dye_terminator
2404
+ Template 22ak65e10
2405
+ Clone bK216E10
2406
+ Sequencing_vector "m13mp18"
2407
+ Seq_vec SVEC 1 41 "M13mp"
2408
+ Tag DONE 361 361 "AUTO-EDIT: replaced C by g at 361 (all_stranded, compound, strong)"
2409
+ Tag DONE 389 389 "AUTO-EDIT: replaced C by a at 389 (terminated, isolated, strong)"
2410
+
2411
+ DNA : 22ak93c2.s1t
2412
+ CTGGGAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCACTCATTTTCTGCCTGG
2413
+ TGTTTATTCTCATCCAAATTCGTATACATGCTCGGTGTGAATGGTTACTCATTATAGTAT
2414
+ ACAATCGACTTTCTCAAACGACCACACATGCTGAATATCTATACATGTGCTAGTTGGACC
2415
+ TCTGATTATCAACGTGAGACTGGTGGCGTATCCCTTTGCAAAATGCTAACAAAAGACCAC
2416
+ AAACGTGTCACATTTCAGAATTGCTTGGAATATTCGCGTGTATAGTACTTACTGGTTCAA
2417
+ GGGTCCAAAACATACCTCTACACACCATCCTGATTCCCTGGGACACTTCCTTTGAACATC
2418
+ ACGTCGGTGCTCAAAAAAGTGTCAGACTGTAGAGCATATCTGGTTTCGGAATGTCGAGAT
2419
+ AGAAGGTCTCAAGCCGTATAAATAAACGAGAGTCTGTATCAAGCACTGCAGGAGAATCAT
2420
+ AACCTCACAGCTATGCTCTCATGGAAACATCTCAATACACCTCGTAGTACTGTACTAATA
2421
+ GTCATGTCGCTGCTTTTTGTTnCCGACAACCAATCTCACGGATTGGTTCGGCCGGTTTGC
2422
+ ATCCCTGATTCCGATCATTGAAGGTCGAACTCCCGGTCCAAGCAATCTCCTGCCCCGGCT
2423
+ CCAGATGACGGGATTCTTGCCTTTCAACACCCCGGCTAATTCATCCTCTCCCTCGACACC
2424
+ GATTTCACGAAATGGGGGTCGGCCCCAACCCGAACATTTGTTTACTGGGTAAAACGGTTC
2425
+ CCCAGTTACCCGTTGGCCCACTCCTGGGCCATTAATTGCCTC
2426
+
2427
+ BaseQuality : 22ak93c2.s1t
2428
+ 4 4 8 8 9 12 12 22 27 30 30 30 30 33 33 35 33 37 37 33 31 30 25 25 27
2429
+ 27 18 18 32 38 32 28 28 9 9 10 10 25 21 27 38 28 25 23 16 12 10 10 11
2430
+ 10 13 12 11 15 14 8 8 11 13 18 21 21 9 7 7 6 11 8 8 8 6 6 8 8 8 8 10 9
2431
+ 9 10 9 14 9 13 11 8 8 8 8 8 11 10 11 14 14 14 21 11 10 10 10 11 17 11
2432
+ 7 7 8 8 9 9 13 14 18 15 9 9 9 8 8 10 12 12 10 10 8 9 12 12 13 14 23 10
2433
+ 10 10 12 11 11 10 10 10 11 14 14 15 15 21 22 10 10 10 10 28 10 9 9 9
2434
+ 10 12 15 18 15 15 12 12 12 12 25 21 24 27 18 13 13 13 14 12 14 12 10
2435
+ 10 10 14 18 14 9 9 11 12 12 10 10 10 10 9 14 14 8 8 8 8 6 6 9 6 6 6 12
2436
+ 18 34 36 38 38 38 38 36 36 33 33 23 23 13 13 15 23 28 20 9 6 6 4 6 10
2437
+ 8 7 7 9 10 9 13 13 10 10 9 8 8 6 6 8 8 11 12 10 9 9 9 6 4 6 6 8 6 7 11
2438
+ 10 9 7 8 17 18 21 19 17 11 8 8 9 11 11 15 12 8 8 8 9 9 15 15 10 10 9
2439
+ 10 7 7 8 12 10 10 9 9 9 9 10 11 10 8 8 8 7 12 7 7 7 8 9 8 9 10 10 9 10
2440
+ 8 6 6 6 6 7 6 9 10 10 10 7 6 6 6 8 6 6 9 10 13 12 12 9 12 15 14 14 8 8
2441
+ 8 9 10 10 9 9 9 9 8 6 6 10 8 13 6 6 6 11 16 18 12 11 8 10 12 8 7 9 7 7
2442
+ 7 7 9 9 7 7 10 10 12 10 20 9 7 7 7 8 8 8 9 7 7 7 11 9 13 19 15 12 7 8
2443
+ 7 6 6 6 6 6 6 6 10 13 8 6 6 8 6 8 10 10 12 7 7 7 7 7 7 8 10 9 9 8 7 6
2444
+ 6 8 6 6 8 9 9 10 7 7 8 8 8 8 7 7 8 7 7 7 7 7 9 8 8 15 8 8 6 6 6 6 6 6
2445
+ 7 10 9 9 8 7 8 8 10 7 7 8 8 11 11 11 8 6 6 6 6 6 12 6 6 6 6 6 7 11 12
2446
+ 9 7 10 11 8 8 7 8 11 8 7 7 7 6 8 6 6 8 6 8 7 7 8 7 7 7 8 9 7 7 7 7 7 7
2447
+ 9 7 7 7 6 13 4 7 7 7 12 23 21 14 12 9 8 9 8 4 4 4 6 6 6 7 6 8 4 4 4 6
2448
+ 6 6 6 6 6 8 6 7 10 9 9 9 8 6 4 4 4 6 8 6 6 6 6 8 6 6 8 8 8 8 8 8 6 6 8
2449
+ 11 10 7 7 7 7 9 9 9 8 7 11 8 7 7 8 9 8 8 7 7 7 7 7 7 7 7 8 7 7 7 7 8 7
2450
+ 7 8 7 7 7 7 8 7 7 7 7 7 7 6 6 7 7 6 6 6 8 9 8 7 7 7 7 8 8 12 9 7 7 7 7
2451
+ 6 7 8 7 7 7 8 8 6 6 6 6 6 6 7 7 9 12 10 14 8 9 8 6 6 6 6 6 6 9 10 10 9
2452
+ 6 6 6 6 8 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 7 7 7 6 6 6 4 6 6 6 6 8 6
2453
+ 6 6 6 6 8 8 10 7 4 7 4 4 4 6 6 6 6 6 6 6 6 6 6 6 7 7 9 9 8 8 7 9 9 7 7
2454
+ 7 8 6 6 6 6 6 6 6 6 6 6 8 8 8 8 8 8 8 7 7 6 6 6 6 7 8 9 9 9 9 7 7 7 7
2455
+ 7 7 7 6 6 6 6
2456
+
2457
+ Sequence : 22ak93c2.s1t
2458
+ Is_read
2459
+ Padded
2460
+ Staden_id 13
2461
+ Clipping QUAL 44 45
2462
+ Align_to_SCF 1 822 1 822
2463
+ SCF_File 22ak93c2.s1tSCF
2464
+ Primer Universal_primer
2465
+ Strand Forward
2466
+ Dye Dye_terminator
2467
+ Template 22ak93c2
2468
+ Clone bK216E10
2469
+ Sequencing_vector "m13mp18"
2470
+ Seq_vec SVEC 1 43 "M13mp1"
2471
+
2472
+ DNA : 22am84d9.s1
2473
+ CAACCCGCACACCTGCACGTCGACTCTAGAGGATCCCCATAAATAAATAAATtAgCTGGG
2474
+ TGTGATGTCACATGCCTGTgATCCCAGCTACTTGGGAgGCTGAGACAGAAGGATCGCTTG
2475
+ AGTCCAGGAGGTCAAGGCTGTAGCGAGTCATGTTCGCCCCACTGCACTCCAGCCTGGGCG
2476
+ ACACAATGAGACCCTGTCTCAAAAACAACAACAACAAAAGGCATCTCTACCTACCTGAGA
2477
+ CCTCTTGGTATCGCTAGGCAGAAGCAAACCGCCTGTACTTGTATTAAAGGTCCGGGTCTT
2478
+ GGTTTTCACTGGTTCATTTGTTTCCCGATAGAGCTTTATTGGAGGAGGCTTGAGAGCCTT
2479
+ CTGGACCAGATTATAAATGCCCACAGAGATCACTATATCTTTGTTGAGCTTCAAGCTTTA
2480
+ ACCTGAGGGCAGTAAAAAATGGAAGAGCATGAACTGGCAAAATGCACTTTTCTATCTAAA
2481
+ ATTCC
2482
+
2483
+ BaseQuality : 22am84d9.s1
2484
+ 4 9 9 9 9 10 10 9 9 9 9 10 10 10 8 8 6 6 10 8 11 12 15 14 13 15 11 11
2485
+ 11 12 14 10 10 10 17 23 31 31 21 20 13 21 11 10 10 16 14 14 14 23 11
2486
+ 11 10 10 10 11 11 12 32 18 23 23 32 32 34 37 34 37 37 38 27 18 18 17
2487
+ 24 30 32 32 10 10 11 23 23 38 37 37 37 37 37 37 37 37 37 37 32 32 22
2488
+ 22 22 32 28 40 37 37 37 45 32 32 28 22 22 12 16 16 32 32 37 37 37 37
2489
+ 28 28 28 32 32 30 30 27 29 29 34 37 37 37 37 37 37 37 37 37 28 22 20
2490
+ 30 30 29 37 34 37 37 37 37 37 37 37 37 37 37 37 45 45 37 37 37 37 45
2491
+ 37 37 34 34 34 33 31 33 34 37 27 27 13 13 13 21 32 34 36 31 31 33 19
2492
+ 17 14 9 9 13 24 27 27 33 33 33 33 33 33 33 33 33 36 36 37 37 37 37 37
2493
+ 45 45 36 34 34 28 31 31 34 34 37 38 34 38 38 33 30 30 30 30 30 33 34
2494
+ 37 37 37 34 34 34 34 34 35 35 33 33 33 33 33 33 34 30 30 30 30 30 30
2495
+ 33 33 30 30 30 34 34 34 34 31 31 31 36 36 34 31 17 13 8 8 15 18 27 31
2496
+ 28 28 28 28 28 30 31 35 35 34 34 34 34 33 33 33 34 34 38 38 38 37 32
2497
+ 28 22 19 19 25 27 37 30 30 30 28 28 28 38 37 34 34 37 31 31 25 24 28
2498
+ 30 30 28 28 28 28 28 28 34 34 34 33 33 31 31 27 24 18 15 18 18 23 23
2499
+ 28 27 22 18 11 11 16 21 24 33 34 23 23 8 8 9 12 14 17 20 20 20 25 25
2500
+ 27 27 23 16 11 15 14 9 9 19 17 18 22 18 11 11 11 12 17 18 31 31 31 31
2501
+ 27 27 33 29 22 18 17 17 20 20 17 13 11 11 11 6 8 14 17 22 22 25 25 20
2502
+ 20 20 17 14 4 4 4 14 15 21 22 18 11 12 7 7 7 7 7 12 21 18 15 13 9 9 15
2503
+ 12 8 6 6 6 6 9 6 6 6 12 14 13 13 17 21 21 24 21 22 18 14 18 16 14 14
2504
+ 10 6 7 6 13 8 8 7
2505
+
2506
+
2507
+ Sequence : 22am84d9.s1
2508
+ Is_read
2509
+ Padded
2510
+ Staden_id 7
2511
+ Clipping QUAL 39 427
2512
+ Align_to_SCF 1 485 1 485
2513
+ SCF_File 22am84d9.s1SCF
2514
+ Primer Universal_primer
2515
+ Strand Forward
2516
+ Dye Dye_primer
2517
+ Template 22am84d9
2518
+ Clone bK216E10
2519
+ Sequencing_vector "m13mp18"
2520
+ Seq_vec SVEC 1 38 "M13mp18"
2521
+ Tag ALUS 41 210
2522
+ Tag DONE 53 53 "AUTO-EDIT: replaced C by t at 53 (all_stranded, isolated, strong)"
2523
+ Tag DONE 55 55 "AUTO-EDIT: replaced C by g at 55 (all_stranded, isolated, strong)"
2524
+ Tag DONE 80 80 "AUTO-EDIT: replaced C by g at 80 (all_stranded, isolated, strong)"
2525
+ Tag DONE 98 98 "AUTO-EDIT: replaced C by g at 98 (all_stranded, isolated, strong)"
2526
+ Tag AMBG 338 360 "AUTOEDIT: Check this edit cluster!"
2527
+ Tag CONF 413 413 "AUTOEDIT: possible compression"
2528
+
2529
+ DNA : 22ak66f10.s1lt
2530
+ CCCGCGGGGGAGGAGGGAnGGGGGGAGAGGAGnAnAAnnnnnnnnnnnnnAGCCCCAAnn
2531
+ nAnnCCCTCTTnnnnnnnnAGACGGGGGGnGCCCTGGGnGnGCnTnnnnnnnnnnTTGGT
2532
+ TGAGGTGnCGAGCAAATCTTGCATGCCTGtcCGTCTACTCtAGAGGATCCCACAAATGAA
2533
+ CCAGTGAAAACCAAGACCCGGACCTTTAATACAAGTACAGGCGGTTTGCTTCTGCCTAGC
2534
+ GATACCAAGAGGTCTCAGGTAGGTAGAGATGCCTTTTGTTGTTGTTGTTTTTGAGACAGG
2535
+ GTCTCATTGTGTCGCCCAGGCTGGAGTGCAGTGGGGCGAACATGACTCGCTACAGCCTTG
2536
+ ACCTCCTGGACTCAAGCGATCCTTCTGTCTCAGCCTCCCAAGTAGCTGGGATCACAGGCA
2537
+ TGTGACATCACACCCAGCTAATTTATTTATTTATTTATTTTTTAAGAGACTGGATCGACT
2538
+ GGGCACAGTGGCTCATGCCTGTAATCCCAnCACTTTGGGAGGCCGAGGCAGGTGGATTAC
2539
+ CGAGGTCAGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCCCATCTCTACTAAAAA
2540
+ TACAAAATGAGCTGGGCATGGTGGTGCATGCCTGTAATCCCAGCTACTCCGGAGGCTGAG
2541
+ GCAnGAGAATCCCTTGAACCCGGGAGGCAAAAGTTGCGGTGAGCCGAAATCTCGCCATTG
2542
+ CGCTCCAGCCTGGGCAACAAAAGCGTAACTCCGTCTCCnnnnnnnnAAAAAAAGAGACGG
2543
+ TGTTTCGCTATGTTGCCTGGGCTGGGTGTCAAACTCCTGGGTCTTAAAGTAATCCCCCCC
2544
+ TGCTTTGGGTTCCCCCAAATTGCTGGGGGTGGCTGGGATTCCCGAACTTTGGGGCCACTG
2545
+ TTGCCCCAGCCGTTTTTTTGGTTTCGTTTTGGTTTTGGTTTTGTTTTAATAAAAAAATAA
2546
+ AGGGTCTCACCTATGTT
2547
+
2548
+ BaseQuality : 22ak66f10.s1lt
2549
+ 6 6 4 6 8 8 11 8 8 6 6 11 4 4 4 12 6 4 4 4 8 7 6 6 6 6 6 6 6 8 4 4 4 4
2550
+ 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 7 7 6 6 6 8 4 4 4 4 4 4 4 4 6 4 6 6
2551
+ 8 4 4 4 4 4 4 4 4 4 4 4 4 6 6 6 6 6 6 4 4 4 6 6 4 4 8 12 4 4 4 4 4 4 4
2552
+ 4 4 4 4 4 4 4 4 4 4 4 4 6 6 4 4 4 4 4 4 8 6 4 4 4 4 4 4 15 17 8 4 4 6
2553
+ 9 21 21 30 28 30 33 33 21 18 8 8 8 10 15 23 21 26 26 19 14 7 7 8 8 10
2554
+ 18 21 18 10 9 9 18 14 24 24 29 32 34 37 34 36 32 32 30 30 30 34 38 44
2555
+ 45 37 37 34 27 27 15 15 23 32 40 40 37 37 34 45 45 45 45 45 42 25 25
2556
+ 25 26 26 20 23 28 30 33 33 33 34 34 34 34 37 45 45 45 45 49 49 49 49
2557
+ 49 45 45 43 38 38 37 37 40 37 37 34 27 27 12 10 18 14 32 27 27 27 29
2558
+ 29 12 18 10 15 10 21 10 14 10 19 19 41 45 45 45 45 45 40 40 34 37 37
2559
+ 45 45 45 45 45 45 45 45 45 40 51 37 37 25 22 18 27 22 25 25 29 29 28
2560
+ 33 33 40 37 37 37 37 37 37 34 37 37 42 35 32 30 30 37 38 45 45 30 30
2561
+ 30 33 31 33 32 37 45 45 45 32 30 30 37 33 37 37 40 37 37 37 37 37 37
2562
+ 37 37 37 37 34 34 34 37 45 45 45 45 45 45 45 45 49 45 45 45 45 33 33
2563
+ 29 29 33 33 33 33 33 37 40 45 45 45 45 45 45 45 35 35 35 35 35 40 38
2564
+ 45 45 45 37 37 32 22 14 14 14 32 45 45 45 38 37 31 28 27 30 28 27 22
2565
+ 27 27 31 31 34 31 28 28 27 31 33 33 33 33 33 33 32 28 28 30 34 37 37
2566
+ 35 35 35 37 37 33 33 33 33 33 33 31 31 30 30 33 31 31 36 33 33 33 34
2567
+ 25 25 10 10 10 19 19 34 34 34 34 31 31 34 34 36 38 30 30 26 23 30 31
2568
+ 34 32 45 37 37 37 37 33 33 40 40 40 36 33 31 31 31 33 33 23 22 4 4 4
2569
+ 18 23 32 34 36 36 32 28 21 21 21 29 20 28 25 24 18 25 21 27 27 32 32
2570
+ 34 31 31 34 37 37 37 37 32 32 10 21 10 25 28 32 29 34 28 31 27 27 28
2571
+ 22 22 27 29 32 32 37 37 37 37 37 37 37 37 35 34 34 34 31 27 27 19 19
2572
+ 30 34 37 38 34 34 34 34 34 40 38 38 38 38 37 37 37 35 35 32 28 28 28
2573
+ 30 30 30 33 30 30 22 22 22 32 32 35 35 34 34 35 35 34 37 37 38 30 24
2574
+ 24 24 24 24 31 38 38 32 27 27 27 35 25 18 12 9 9 11 21 23 18 12 6 6 8
2575
+ 8 8 9 10 17 10 14 8 9 8 7 4 4 4 16 14 14 12 15 8 8 11 15 14 25 25 25
2576
+ 28 33 33 33 21 29 7 7 7 8 8 7 8 9 9 8 11 24 22 31 25 23 23 15 13 10 7
2577
+ 9 9 8 8 12 7 7 7 16 11 18 14 25 25 22 19 16 17 14 8 9 10 13 15 27 28
2578
+ 31 23 22 14 14 13 15 15 23 21 8 8 6 6 6 6 9 18 20 28 22 22 20 17 14 9
2579
+ 4 4 4 4 4 4 4 4 4 4 16 10 13 20 23 23 12 9 9 9 17 10 9 7 8 6 6 8 4 4 7
2580
+ 12 13 14 7 8 8 8 8 8 18 21 16 9 10 9 7 9 7 9 8 10 7 7 7 12 9 9 7 7 8 8
2581
+ 6 6 8 8 8 6 8 6 6 6 6 7 6 11 6 8 6 6 6 6 6 8 9 9 8 9 12 8 8 8 7 8 8 7
2582
+ 8 6 6 6 9 8 6 6 6 10 13 11 7 9 7 7 7 7 7 8 9 7 7 9 9 7 4 4 4 7 11 7 7
2583
+ 9 8 7 6 6 6 6 6 8 8 10 9 8 8 6 6 6 6 7 6 6 4 4 4 4 8 6 8 13 16 20 8 8
2584
+ 7 7 7 7 7 7 7 8 12 8 8 6 6 6 6 6 6 6 6 6 6 9 8 8 6 6 6 8 6 9 14 9 9 8
2585
+ 9 7 6 6 8 6 6 8 7 6 6 8 6 6 7 7 7 7 7 7 8 8 4 4
2586
+
2587
+
2588
+ Sequence : 22ak66f10.s1lt
2589
+ Is_read
2590
+ Padded
2591
+ Staden_id 9
2592
+ Clipping QUAL 140 640
2593
+ Align_to_SCF 1 977 1 977
2594
+ SCF_File 22ak66f10.s1ltSCF
2595
+ Primer Universal_primer
2596
+ Strand Forward
2597
+ Dye Dye_primer
2598
+ Template 22ak66f10
2599
+ Clone bK216E10
2600
+ Sequencing_vector "m13mp18"
2601
+ Seq_vec SVEC 1 3 "M13mp18"
2602
+ Tag DONE 150 150 "AUTO-EDIT: replaced C by t at 150 (all_stranded, compound, strong)"
2603
+ Tag DONE 151 151 "AUTO-EDIT: replaced G by c at 151 (all_stranded, compound, strong)"
2604
+ Tag DONE 161 161 "AUTO-EDIT: replaced A by t at 161 (all_stranded, compound, strong)"
2605
+ Tag ALUS 283 462
2606
+ Tag ALUS 477 767
2607
+
2608
+ DNA : 22ak70h4.r1t
2609
+ TGCCGCCTTAGATTACAGCTCTACACGGTACCTCTATCGGGAAACAAATGAACCAgTGAA
2610
+ AACCAAGACCCGGACCTTTAATACAAGTACAGGCGGTTTGCTTCTGCCTAGCGATACCAA
2611
+ GAGGTCTCAGGTAGGTAGAGATGCCTTTTGTTGTTGTTGTTTTTGAGACAGGGTCTCATT
2612
+ GTGTCGCCCAgGCTGGAGTGCAGTGGGGCGAACATGACTCGCTACAGCCTTGACCTCCTG
2613
+ GACTCAAGCGATCCTTCTGTCTCAGCCTCCCAAGTAGCTGGGATCACAGGCATGTGACAT
2614
+ CACACCCAGCTAATTTATTTATTTATTTATTTTTTAAGAGACTGGATCGACTGGGCACAG
2615
+ TGGCTCATGCCTGTAATCCCACCACTTTGGGAGGCCGAGGCATGTGGATTACCGAGGTTC
2616
+ GGAGTTCAAGACCAGCCTGACCCTTCTGGAGAAACCCCATCTCCACTTTAAATACCAAAT
2617
+ GAAGCTGGGCCTGGTGGTGCCTGCCTGTAATCCCCCCTTCTCAGGATGCTGAAGCAGGAC
2618
+ ATTCCTTGAACCCCGGAAGCACATGTTGCCGTGACCCAATATCCCCCCTTGTCCCTCACC
2619
+ CCGGGCCACCAAACCTTATTCCCTCCCAAATAAAAAAAAAAAAAAACGGGTTCCCTTTTT
2620
+ TGCCGGGGGGGGTTTCTACCCC
2621
+
2622
+ BaseQuality : 22ak70h4.r1t
2623
+ 4 4 4 4 4 8 6 6 8 8 12 6 7 11 13 12 6 6 6 6 6 6 9 10 10 10 21 21 26 31
2624
+ 27 27 13 14 11 11 11 12 19 20 27 18 18 23 23 16 10 10 14 28 18 18 11
2625
+ 11 9 9 9 10 10 10 21 21 27 23 18 10 9 9 14 18 32 31 37 34 28 28 22 19
2626
+ 19 27 24 21 18 17 9 9 12 19 24 28 30 30 30 33 33 30 30 37 40 40 45 37
2627
+ 41 41 44 44 45 32 42 37 32 32 32 32 37 31 31 33 21 21 11 10 22 18 32
2628
+ 24 27 27 27 27 11 14 10 23 12 23 9 10 10 18 11 25 25 34 45 37 37 34 34
2629
+ 34 34 34 34 34 33 33 33 34 34 34 34 33 34 28 31 25 21 23 21 21 13 13
2630
+ 15 15 17 23 21 26 26 31 28 32 23 23 12 22 25 21 19 9 8 8 17 21 23 23
2631
+ 20 18 17 27 23 21 27 30 38 38 28 28 17 17 13 18 19 27 25 25 28 28 28
2632
+ 28 25 31 26 26 18 15 13 9 9 15 19 23 25 31 36 36 36 36 36 36 36 36 24
2633
+ 19 12 13 18 24 31 31 29 29 31 29 29 33 36 40 44 36 33 34 34 37 36 36
2634
+ 45 45 34 30 28 28 28 22 32 24 22 22 32 32 36 37 27 27 14 25 25 29 23
2635
+ 22 19 17 19 19 24 25 27 24 18 18 16 16 16 24 22 30 30 28 28 30 27 27
2636
+ 23 20 22 25 25 25 21 22 21 16 14 19 17 17 19 20 17 11 11 11 11 12 24
2637
+ 24 22 20 34 17 17 23 23 31 34 27 27 17 13 15 27 27 34 31 31 24 22 19
2638
+ 16 16 15 32 23 27 18 9 9 9 13 9 14 20 22 21 21 19 17 14 8 6 6 8 8 9 11
2639
+ 14 8 7 11 15 14 9 9 9 12 8 10 6 8 8 6 4 4 4 14 15 23 21 13 13 11 13 11
2640
+ 11 6 8 8 6 6 6 7 6 6 6 6 7 6 6 11 15 17 27 20 17 17 13 21 13 14 4 4 8
2641
+ 6 6 6 6 8 6 8 11 7 8 8 10 10 19 24 16 16 9 9 12 7 7 8 8 8 8 6 6 11 15
2642
+ 11 10 9 9 11 14 15 11 8 7 7 7 7 12 9 10 10 8 8 14 10 10 18 21 21 18 16
2643
+ 10 9 11 9 9 14 10 10 8 8 9 8 8 12 14 10 10 11 11 10 9 8 7 7 7 7 4 4 4
2644
+ 12 10 14 10 8 14 6 6 6 7 6 6 6 6 6 10 8 9 7 7 7 9 14 15 10 10 10 10 9
2645
+ 8 13 8 4 4 4 4 4 6 6 12 6 6 10 8 8 8 6 9 12 7 6 6 6 8 8 9 7 7 7 8 9 8
2646
+ 8 6 6 6 6 6 4 6 6 6 6 7 8 8 6 6 8 8 8 8 9 9 8 10 11 9 8 8 8 8 6 6 11 9
2647
+ 9 10 9 12 8 6 6 6 10 12 10 10 8 8 8 7 10 13 8 13 19 18 13 6 6 6 6 8 6
2648
+ 7 12 7 7 10 10 7 7 7 7 7 7 9 13 6 10 7 7 12 11 7 6 6 6 6 8 8 8 4
2649
+
2650
+
2651
+ Sequence : 22ak70h4.r1t
2652
+ Is_read
2653
+ Padded
2654
+ Staden_id 15
2655
+ Clipping QUAL 28 377
2656
+ Align_to_SCF 1 682 1 682
2657
+ SCF_File 22ak70h4.r1tSCF
2658
+ Primer Universal_primer
2659
+ Strand Reverse
2660
+ Dye Dye_terminator
2661
+ Template 22ak70h4
2662
+ Clone bK216E10
2663
+ Sequencing_vector "m13mp18"
2664
+ Seq_vec SVEC 1 27 "M13mp18"
2665
+ Tag DONE 56 56 "AUTO-EDIT: replaced C by g at 56 (all_stranded, isolated, strong)"
2666
+ Tag ALUS 155 334
2667
+ Tag DONE 191 191 "AUTO-EDIT: replaced T by g at 191 (all_stranded, isolated, strong)"
2668
+ Tag ALUS 349 579
2669
+
2670
+ Sequence : Contig2
2671
+ Is_contig
2672
+ Padded
2673
+ Assembled_from 22ak93c2.r1t 1 293 39 331
2674
+ Assembled_from 22ak93c1.r1ta 1 305 31 335
2675
+ Assembled_from 22am91h5.r1t 110 439 32 361
2676
+ Assembled_from 22aq40a8.s1 221 570 39 388
2677
+ Assembled_from 22an02b10.s1t 252 666 32 446
2678
+ Assembled_from 22ak67d6.r1t 260 522 30 292
2679
+ Assembled_from 22ak64b1.s1t 331 707 40 416
2680
+ Assembled_from 22ak81h10.s1t 331 713 36 418
2681
+ Assembled_from 22ak66c6.s1t 337 848 37 548
2682
+ Assembled_from 22ak93c1.s1lt 1024 482 56 598
2683
+ Assembled_from 22ak64e2.s1t 866 487 41 420
2684
+ Assembled_from 22ak70f10.s1t 554 1027 35 508
2685
+ Assembled_from 22ak92c4.s1t 635 1088 37 490
2686
+ Assembled_from 22ap08f1.s1t 639 777 17 155
2687
+ Assembled_from 22ai01c4.s1t 682 1200 35 553
2688
+ Assembled_from 22ak93c1.s1t 1036 735 44 345
2689
+ Assembled_from 22ak65e10.s1t 1499 966 42 575
2690
+ Assembled_from 22ak93c2.s1t 1036 1035 44 45
2691
+ Assembled_from 22am84d9.s1 1488 1100 39 427
2692
+ Assembled_from 22ak66f10.s1lt 1174 1674 140 640
2693
+ Assembled_from 22ak70h4.r1t 1190 1539 28 377
2694
+
2695
+ DNA : Contig2
2696
+ TTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTATAnACTGTGCGTGCGC
2697
+ CACCATGCCTGGCTAATTTTTGTATTTTTAGTAGGGATGGGGTTTCACCATGTTGGCCAA
2698
+ GCTGGTCTCGAGCTCCTGACCTAGGATTACAGGCCTAAGCCACCGCACCCGGCATGATGG
2699
+ GTCTTTATTCTTCAAAGCAGGAGGAAGGGATCCTAGAAAAACAGAGACAAGGCCAAACAT
2700
+ GGTAGCTCACACCTGTAATnnnnnCACTTTGGGAGGCCAGTGCGGGTGAATCACGAnGTC
2701
+ AGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAnCACCGTCTCTACTAAAATAAAAAGA
2702
+ AATTAGCTGGGTGTCGTGGCAGGTGCnTGTAATCCCAGCCACTTGGGAAGCTGAGGCAGG
2703
+ AGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCGACTGCACTC
2704
+ CAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGACAAGACnACT
2705
+ AGTACAGTACTTACAGGGTTATTATGATGATTAAATGAGAGAATAGCTGTGAGGTGATTG
2706
+ ATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATACGGGTTGAGTGTnTCTAATC
2707
+ TGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAATG
2708
+ TTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGGGTTGGGAATATTC
2709
+ AACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAATCTGAAACATTTC
2710
+ TGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCTGTAGTACGTTCTTTATCATTG
2711
+ TTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAGCAAATTCGATTGTA
2712
+ TTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAGGATTATTGGAGAGAATAAAAC
2713
+ AGTGAAGCTTTGGTGTTATGAGGGAATTTTAGATAGAAAAGTGCAGTTTTTCAGTTCATG
2714
+ CTCTTTCATTTTTTACTCCCTCAGGTTAAAGCTnGAAGCTCAACAAAGATATAGTGATCT
2715
+ CTGTGGGCATTTATAATCTGGTCCAGAAGGCTCTnnAnnCnnnTCCnnnnnnnCTnnAnn
2716
+ nnnnnACAAATGAACCAGTGAAAACCAAGACCCGGACCTTTAATACAAGTACAGGCGGTT
2717
+ TGCTTCTGCCTAGCGATACCAAGAGGTCTCAGGTAGGTAGAGATGCCTTTTGTTGTTGTT
2718
+ GTTTTTGAGACAGGGTCTCATTGTGTCGCCCAGGCTGGAGTGCAGTGGGGCGAACATGAC
2719
+ TCGCTACAGCCTTGACCTCCTGGACTCAAGCGATCCTTCTGTCTCAGCCTCCCAAGTAGC
2720
+ TGGGATCACAGGCATGTGACATCACACCCAGCTAATTTATTTATTTATTTATTTTTTAAG
2721
+ AGACTGGATCGACTGGGCACAGTGGCTCATGCCTGTAATCCCAnCACTTTGGGAGGCCGA
2722
+ GGCAGGTGGATTACCGAGGTCAGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCCC
2723
+ ATCTCTACTAAAAATACAAAATGAGCTGGGCATGGTGGTGCATGCCTGTAATCC
2724
+
2725
+ BaseQuality : Contig2
2726
+ 4 4 8 4 6 10 13 21 24 25 33 33 33 30 27 21 15 27 19 30 30 33 33 30 21
2727
+ 21 10 9 17 11 27 27 37 38 33 35 35 35 35 44 45 45 38 37 37 45 45 45 43
2728
+ 43 43 45 45 45 45 45 45 45 21 21 23 30 30 34 37 37 38 45 32 45 37 41
2729
+ 37 45 30 45 45 34 34 34 32 29 22 32 32 45 45 45 33 28 28 37 37 34 34
2730
+ 34 35 34 34 34 34 34 34 37 37 40 41 37 34 37 37 37 32 24 22 27 29 25
2731
+ 27 20 21 21 21 27 45 41 40 40 40 42 34 34 37 41 45 51 45 45 37 30 37
2732
+ 41 41 37 36 28 30 30 30 22 33 33 35 33 33 41 51 51 45 39 39 39 30 28
2733
+ 33 34 34 39 34 41 34 34 41 33 33 39 39 39 33 33 33 30 33 33 33 30 29
2734
+ 19 19 24 25 32 33 45 36 36 36 36 41 41 30 30 30 37 37 43 51 51 51 51
2735
+ 45 37 37 30 37 37 37 51 51 51 51 51 37 37 28 28 10 10 10 13 10 10 10 9
2736
+ 9 9 21 21 28 37 37 37 33 33 33 33 33 33 34 34 34 33 33 33 33 21 25 25
2737
+ 22 22 29 29 33 33 33 33 31 31 27 28 17 23 23 28 26 24 32 10 10 10 15
2738
+ 15 26 32 32 37 45 45 32 45 33 26 24 32 37 37 35 34 37 35 37 34 37 37
2739
+ 40 37 37 45 45 38 38 45 45 40 37 37 37 34 30 30 28 28 28 30 37 37 34
2740
+ 34 34 34 34 33 31 31 25 21 19 22 21 25 30 32 32 30 22 22 25 28 24 25
2741
+ 26 17 17 23 27 27 33 31 31 34 38 38 38 38 34 26 17 15 8 8 11 16 25 31
2742
+ 32 33 29 26 32 29 33 34 34 34 36 33 34 34 31 27 31 34 38 45 45 36 37
2743
+ 37 37 36 36 38 34 32 28 25 22 22 21 17 18 29 29 23 23 22 27 25 20 18
2744
+ 11 11 15 15 16 23 21 17 19 24 24 24 31 31 31 33 33 33 31 33 33 29 29
2745
+ 29 29 29 31 31 31 23 24 19 12 10 16 10 10 20 10 10 13 15 30 23 29 23
2746
+ 28 18 10 10 16 21 18 19 19 24 24 12 11 9 9 10 21 23 31 31 33 28 28 14
2747
+ 17 17 28 21 30 24 30 28 22 26 23 19 10 10 12 23 22 23 19 12 10 9 9 10
2748
+ 19 24 29 30 34 34 34 34 34 29 25 25 32 26 31 31 32 20 17 15 12 12 4 4
2749
+ 4 17 23 33 30 24 18 13 18 16 12 16 8 9 9 19 15 15 4 4 4 7 11 6 6 6 7 8
2750
+ 9 17 12 13 19 23 25 13 13 13 19 21 29 32 30 26 20 20 12 9 9 8 9 8 8 15
2751
+ 24 17 15 8 8 9 17 16 4 4 4 8 8 13 15 23 14 9 8 8 10 10 17 23 21 17 12
2752
+ 13 22 20 19 15 11 11 9 8 8 8 8 8 8 10 9 9 7 8 8 10 12 10 10 12 4 4 4 4
2753
+ 4 4 10 10 10 10 10 9 8 8 7 8 9 11 9 9 4
2754
+
2755
+