bio-assembly 0.0.2 → 0.1.0

data/Gemfile

@@ -3,6 +3,8 @@ source "http://rubygems.org"
 # Example:
 #   gem "activesupport", ">= 2.3.5"
 
+gem "bio", ">= 1.4.1"
+
 # Add dependencies to develop your gem here.
 # Include everything needed to run rake, tests, features, etc.
 group :development do

data/LICENSE.txt

@@ -1,4 +1,4 @@
-Copyright (c) 2010 Chase Miller
+Copyright (c) 2010 Chase Miller and Francesco Strozzi
 
 Permission is hereby granted, free of charge, to any person obtaining
 a copy of this software and associated documentation files (the

data/README.rdoc

@@ -2,14 +2,54 @@
 
 BioRuby plugin for parsing, writing, and maniuplating assembly data
 
+Currently supports ACE and CAF formats
+
 == Install
 
 gem install bio-assembly
 
 == Usage
 
-Examples on my blog:
-http://chasemiller4.blogspot.com/2010/10/bioruby-ace-parser-example.html
+ require 'bio-assembly'
+ 
+ # ace file path
+ asm = Bio::Assembly.new("data.ace", :ace)
+ 
+ # iterate through contigs (streams each contig)
+ asm.each_contig do |contig|
+  
+   # print name and consensus seq
+   puts contig.name
+   puts contig.consensus_seq
+  
+   # iterate through reads
+   contig.each_read do |read|
+      
+      # print name, sequence and orientation
+      puts read.name
+      puts read.seq
+      puts read.orientation
+     
+      # print the postion of the read
+      # in relation to the consensus seq
+      puts read.from
+      puts read.to
+     
+      # print the portion of the read
+      # that was used to create the consensus
+      puts read.clear_range_from
+      puts read.clear_range_to
+     
+   end
+  
+   # grab the reads that make up a particular region of the contig
+   reads_in_region = contig.find_reads_in_range(10, 50)
+  
+ end
+ 
+ # experimental - output ace
+ file = File.new('out.ace', 'w')
+ file.puts asm.to_ace
 
 == Contributing to bio-assembly
  
@@ -23,6 +63,6 @@ http://chasemiller4.blogspot.com/2010/10/bioruby-ace-parser-example.html
 
 == Copyright
 
-Copyright (c) 2010 Chase Miller. See LICENSE.txt for
+Copyright (c) 2011 Chase Miller and Francesco Strozzi. See LICENSE.txt for
 further details.
 

data/Rakefile

@@ -18,7 +18,7 @@ Jeweler::Tasks.new do |gem|
   gem.summary = "BioRuby Assembly plugin"
   gem.description = "bioruby plugin to parse, write, and manipulate assembly data"
   gem.email = "chmille4@gmail.com"
-  gem.authors = ["Chase Miller"]
+  gem.authors = ["Chase Miller","Francesco Strozzi"]
   # Include your dependencies below. Runtime dependencies are required when using your gem,
   # and development dependencies are only needed for development (ie running rake tasks, tests, etc)
   #  gem.add_runtime_dependency 'jabber4r', '> 0.1'

data/VERSION

@@ -1 +1 @@
-0.0.2
+0.1.0

data/bio-assembly.gemspec

@@ -5,11 +5,11 @@
 
 Gem::Specification.new do |s|
   s.name = %q{bio-assembly}
-  s.version = "0.0.2"
+  s.version = "0.1.0"
 
   s.required_rubygems_version = Gem::Requirement.new(">= 0") if s.respond_to? :required_rubygems_version=
-  s.authors = ["Chase Miller"]
-  s.date = %q{2010-12-27}
+  s.authors = ["Chase Miller", "Francesco Strozzi"]
+  s.date = %q{2011-01-12}
   s.description = %q{bioruby plugin to parse, write, and manipulate assembly data}
   s.email = %q{chmille4@gmail.com}
   s.extra_rdoc_files = [
@@ -25,13 +25,17 @@ Gem::Specification.new do |s|
     "Rakefile",
     "VERSION",
     "bio-assembly.gemspec",
+    "data/example.caf",
     "data/example1.ace",
     "lib/bio-assembly.rb",
     "lib/bio-assembly/ace.rb",
+    "lib/bio-assembly/caf.rb",
     "lib/bio-assembly/contig.rb",
+    "lib/bio-assembly/maf.rb",
     "lib/bio-assembly/read.rb",
     "test/helper.rb",
-    "test/test_bio-assembly.rb"
+    "test/test_bio-assembly-ace.rb",
+    "test/test_bio-assembly-caf.rb"
   ]
   s.homepage = %q{http://github.com/chmille4/bioruby-assembly}
   s.licenses = ["MIT"]
@@ -40,7 +44,8 @@ Gem::Specification.new do |s|
   s.summary = %q{BioRuby Assembly plugin}
   s.test_files = [
     "test/helper.rb",
-    "test/test_bio-assembly.rb"
+    "test/test_bio-assembly-ace.rb",
+    "test/test_bio-assembly-caf.rb"
   ]
 
   if s.respond_to? :specification_version then
@@ -48,12 +53,14 @@ Gem::Specification.new do |s|
     s.specification_version = 3
 
     if Gem::Version.new(Gem::VERSION) >= Gem::Version.new('1.2.0') then
+      s.add_runtime_dependency(%q<bio>, [">= 1.4.1"])
       s.add_development_dependency(%q<shoulda>, [">= 0"])
       s.add_development_dependency(%q<bundler>, ["~> 1.0.0"])
       s.add_development_dependency(%q<jeweler>, ["~> 1.5.2"])
       s.add_development_dependency(%q<rcov>, [">= 0"])
       s.add_development_dependency(%q<bio>, [">= 1.4.1"])
     else
+      s.add_dependency(%q<bio>, [">= 1.4.1"])
       s.add_dependency(%q<shoulda>, [">= 0"])
       s.add_dependency(%q<bundler>, ["~> 1.0.0"])
       s.add_dependency(%q<jeweler>, ["~> 1.5.2"])
@@ -61,6 +68,7 @@ Gem::Specification.new do |s|
       s.add_dependency(%q<bio>, [">= 1.4.1"])
     end
   else
+    s.add_dependency(%q<bio>, [">= 1.4.1"])
     s.add_dependency(%q<shoulda>, [">= 0"])
     s.add_dependency(%q<bundler>, ["~> 1.0.0"])
     s.add_dependency(%q<jeweler>, ["~> 1.5.2"])

data/data/example.caf

@@ -0,0 +1,2755 @@
+DNA : 22ak93c2.r1t
+GTCGCnCATAAGATTACGAGATCTCGAGCTCGGTACCCTTCAAGCGATTCTCCTGCCTCA
+GCCTCCCGAGTAGCTGGGATTATAGACTGTGCGTGCGCCACCATGCCTGGCTAATTTTTG
+TATTTTTAGTAGGGATGGGGTTTCACCATGTTGGCCAAGCTGGTCTCGAGCTCCTGACCT
+AGGATTACAGGCCTAAGCCACCGCACCCGGCATGATGGGTCTTTATTCTTCAAAGCAGGA
+GGAAGGGATCCTAgAAAAACAGAGACAAGGCCAAACATGGTAgCTCACACCTGTAATCCC
+AnCACTTTGGGAGGCCagTGCGGGTGAATCACGAAGTCAGGAGTTCAAGACCACCCTGGC
+CAACATGGTGTAACACCTGCTCTCCTAAAATTAAACAAAATTTCATGGTTTGCGTGGGCC
+GTCTTGTCTCATCACTTCACTCCTGAGGGCCGGCGCCGGAAAGATATCTTGATCTGCGGC
+GCTCCGACCGTTTTCTTTAAACCTTACAACTCCCGACCTCCTCGCCTATCCTCCCTAAAT
+CCTCGCCAGGCTCGCCTGCTTCAGCCACTCTTTCCTTCGCACCCTCCCCTCTCTTCAATA
+TACTTCACCCGCCCATCCTTCACGCCGGCACGTATCCAATCTCTTCTTATCTTTCCGTAT
+CCAAnTCCCTTCTCCCTCTGCCGCGACCTTCGCCATCCCTCTGCGCGTCCTCTTCC
+
+BaseQuality : 22ak93c2.r1t
+4 4 8 4 4 4 4 4 4 4 4 4 6 8 17 21 14 7 6 6 6 7 7 6 8 14 16 21 15 20 20
+24 26 21 18 18 14 14 19 23 10 8 8 15 20 16 29 26 34 29 39 29 31 29 31
+34 32 27 27 25 19 19 24 31 33 36 34 34 34 26 27 22 32 32 36 28 28 15
+15 15 28 28 34 30 12 12 22 27 31 31 31 31 31 23 24 27 21 24 24 29 27
+27 27 34 34 36 38 38 38 36 36 40 36 37 38 45 45 36 34 33 31 31 34 34
+33 33 28 28 27 23 24 11 11 10 10 18 25 21 20 17 17 17 20 15 24 18 24
+26 23 23 18 20 25 23 30 30 30 33 33 37 37 32 37 37 32 45 35 37 37 37
+40 36 49 49 36 36 34 33 20 15 9 9 8 7 12 22 21 28 28 30 33 36 36 36 34
+31 31 25 31 28 26 26 24 20 17 9 11 8 9 10 23 23 31 23 23 15 9 9 15 33
+26 33 33 31 25 25 22 31 24 23 12 10 12 11 9 8 9 7 7 8 8 9 18 12 9 9 18
+20 26 31 21 21 9 8 8 11 13 21 21 23 15 15 15 15 15 17 17 9 7 9 19 20
+21 21 25 25 25 25 25 23 23 9 9 9 21 16 24 24 24 24 26 33 33 33 31 31
+27 15 17 7 4 4 4 16 20 27 33 34 34 23 15 14 8 9 6 6 9 1 14 16 8 11 15
+23 25 34 36 31 33 16 16 6 6 6 9 8 14 9 11 11 9 13 13 10 8 10 9 9 7 8
+20 20 20 14 14 10 10 10 16 8 8 6 6 8 9 10 7 8 8 8 8 6 8 6 6 8 12 9 8 7
+13 10 8 8 9 8 8 8 9 6 6 6 6 8 6 6 6 6 6 10 11 10 12 12 10 7 6 6 7 6 6
+7 8 7 6 6 7 6 8 6 6 8 8 8 8 6 6 6 8 6 6 8 6 6 11 15 9 9 9 9 9 9 9 9 9
+9 8 9 9 6 6 6 7 9 6 6 6 7 7 7 8 9 7 7 9 9 11 8 11 11 11 9 10 9 8 8 8
+10 6 6 9 8 6 6 6 6 8 8 9 11 9 8 8 8 8 8 8 8 9 9 9 10 10 8 6 6 8 6 6 10
+11 10 6 6 8 6 6 6 6 8 11 8 9 8 8 8 8 8 9 6 6 9 8 8 8 6 6 13 15 12 12 8
+8 6 6 8 9 8 8 9 8 8 6 6 6 8 8 6 6 6 8 8 8 6 6 8 6 6 8 6 6 9 8 8 6 6 8
+8 6 6 8 6 7 8 9 10 11 11 10 9 9 8 10 9 10 10 8 9 13 8 8 8 13 13 11 7 7
+7 11 8 8 10 11 10 9 9 14 7 7 10 10 10 8 8 8 8 6 7 8 8 6 6 6 6 6 6 6 6
+6 8 9 10 8 8 6 6 8 8 8 8 8 8 8 8 8 8 6 6 8 8 9 10 10 4 4 4 6 7 6 6 6 6
+10 8 8 9 15 11 6 6 8 8 8 8 8 8 9 9 8 9 7 7 8 6 6 8 13 10 8 8 7 6 8 6 6
+8 6 7 8 8 8 8 8 8 8 4
+
+
+Sequence : 22ak93c2.r1t
+Is_read
+Padded
+Staden_id 11
+Clipping QUAL 39 331
+Align_to_SCF 1 43 1 43
+Align_to_SCF 44 317 45 318
+Align_to_SCF 319 716 319 716
+SCF_File 22ak93c2.r1tSCF
+Primer Universal_primer
+Strand Reverse
+Dye Dye_terminator
+Template 22ak93c2
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 38 "M13mp18"
+Tag ALUS 43 180
+Tag DONE 43 43 "AUTO-EDIT: deleted C at 43  (terminator, isolated, strong)"
+Tag DONE 254 254 "AUTO-EDIT: replaced T by g at 254 (terminator, isolated, strong)"
+Tag ALUS 269 402
+Tag DONE 283 283 "AUTO-EDIT: replaced T by g at 283 (terminated, isolated, strong)"
+Tag AMBG 298 302 "AUTOEDIT: Check this edit cluster!"
+Tag DONE 317 317 "AUTO-EDIT: replaced C by a at 317 (terminated, compound, strong)"
+Tag DONE 318 318 "AUTO-EDIT: inserted g at 318  (terminated, compound, strong)"
+
+DNA : 22ak93c1.r1ta
+GGGGGGATGATACAATTCGAGCTCGGTACCTTCAAGCGATTCTCCTGCCTCAGCCTCCCG
+AGTAGCTGGGATTATAAACTGTGCGTGCGCCACCATGCCTGGCTAATTTTTGTATTTTTA
+GTAGGGATGGGGTTTCACCATGTTGGCCAaGCTGGTCTCGAGCTCCTGACCTAGGATtAC
+AGGCCTAaGCCACCGCACCCGGCATGATGGGTCTTTATTCTTCAAAGCAgGAGGAAGGGA
+TCCTAGAAAAACAGAGACAAgGCCAAACATGGTAGCTCACACCTGTAATCCCAGCACTTT
+GGGAgGCCAgTGCGGGTGAATCACgAAGTCAGGAGTTCAAGACCATCCTGGCCAACATGG
+TGTAACACCGTCTCTCCTAGGATAAACCGAAATCACCTGGGTGTCCTGGCAAGTGCCTGT
+CATCCCTGACCACTTGGAAAGCTGAGCCGAATCCTTATCCCnGAAAGTAAGGTTACGTGA
+ACCCACATCAAACCACTGCGCTCCAGCCCAAAACATCGTGTTATACTCTGTCGCGACATT
+GATGCCCAAAAAACAGGACCGCTATTGGGAGACACTCCCGGGTAATTGGGATTGAGGAGG
+TTAAAGAAAACTCTTGATCTGAGAAAAAAGACCCTATCCTGGCGAGAAAACCACATGTGG
+ACCACACCGCTTCACTTTGTCTAAGCAAAAAACGCCCCACTTGAAGATGACCCCCCCAGC
+TGAAATCTTCCATTAATCCCCGAACATAAAGGTTTAACCCAAAATTTCCTGTTAGACCGC
+CTTCTGAAGTTTCTAAAATTCTATGGGTAGGGACAAAAGTCCCTCGTTGAATTTCTAGGA
+TACTTCCAAATATCCCCCACCTCTCCCCTGGGATCCCGATAGCTTTCTTGTCCCTAAAAA
+AAACTTTGTTCAAACGGAGAAACCACACCCGGGGAAAATTCCCCTTTTCTTGGGGTTTAG
+GTTTCTTTAAATAAATGCTGGGCACCAATCCTCAAATGAGTTCTTACCCAATCCCATATG
+TTTTTAATCCTCAACAACAACCTTATTTCCTTAAGAGACCTCTCCTCGGAAATCATTTCA
+AAACAAAAAAAACACTTAAGCCTTGGGTCTTTGGGACCCCCCAAAAAAAAAACATAAAGG
+GGTGGCACCGTATACGCCTTGCCCATCTTTCTAGTGGAAGATAACGAAACGGTAGCTGGG
+GCCAACCAGGCATCAGTGGGGGATACCGCTAAATGTTGGGGAACACCCAAAAACCCATCT
+GCAGGCACCGCGAATGCGGAAAAGCGCACGCTATCACAAATGGTATATGGGCACC
+
+BaseQuality : 22ak93c1.r1ta
+8 10 8 9 16 9 9 9 11 8 8 9 10 11 11 10 19 24 26 24 26 22 21 17 13 16
+11 11 10 10 14 14 19 22 24 26 32 32 33 29 29 29 27 27 27 27 27 32 32
+25 27 20 25 19 32 27 29 32 32 32 34 34 21 23 12 14 14 27 32 32 29 28
+27 34 31 34 34 34 37 34 32 32 30 30 28 28 28 30 30 33 33 33 30 30 34
+34 34 37 40 38 37 34 32 34 32 37 32 37 37 28 28 28 33 33 33 33 32 30
+30 29 28 21 23 14 12 30 32 45 45 41 41 30 25 22 33 33 34 23 21 18 12
+12 11 13 27 27 23 23 11 11 9 10 11 18 18 30 15 20 12 11 10 20 12 14 14
+32 32 33 28 28 18 23 18 12 12 20 12 16 13 18 14 15 12 32 23 21 10 10 9
+9 10 16 18 21 9 9 9 18 21 14 14 12 10 14 14 23 27 24 19 19 24 28 30 29
+29 34 34 29 25 17 12 12 16 14 14 23 10 10 10 11 15 21 24 18 25 11 11
+15 32 28 34 34 29 32 32 37 37 34 34 31 28 28 25 22 17 14 11 10 10 8 8
+9 14 8 8 12 17 18 15 13 18 17 17 10 10 10 22 16 24 30 24 28 27 32 19
+12 12 12 30 21 17 9 9 9 9 17 16 24 24 23 20 12 12 7 7 7 14 13 10 10 10
+23 23 22 15 17 18 24 24 15 17 11 10 8 8 8 13 20 25 17 17 17 20 20 18
+10 11 12 12 11 25 10 12 12 9 8 8 8 8 8 12 14 9 9 9 9 10 10 8 8 11 9 9
+9 14 20 20 19 15 15 15 15 11 11 6 6 9 8 10 12 12 15 15 10 9 10 11 7 7
+9 11 14 11 11 10 11 9 14 14 23 19 18 14 12 10 10 10 10 10 16 11 11 9 8
+8 9 8 11 11 9 8 8 10 9 12 13 9 9 8 8 8 8 8 6 6 11 6 6 8 11 9 9 9 6 6
+12 9 9 6 6 8 11 9 8 6 7 9 9 7 8 4 4 4 6 8 6 6 8 8 6 6 8 6 8 12 12 14
+10 9 10 11 9 8 7 7 7 7 10 8 8 6 6 8 6 6 8 10 8 6 6 10 8 10 9 10 10 9 8
+8 6 6 6 6 8 8 8 9 9 9 9 7 7 9 9 10 10 10 13 12 9 9 9 9 7 8 8 8 9 8 8 9
+9 9 8 6 6 8 6 6 6 7 7 9 9 10 8 8 7 7 7 9 10 10 8 6 6 6 6 6 10 10 8 6 6
+8 8 8 7 9 7 10 7 11 8 8 8 8 9 9 9 8 8 6 6 10 11 16 8 7 7 7 6 8 6 6 8
+10 11 9 9 9 7 7 8 7 7 7 7 7 8 11 9 16 16 24 22 15 9 8 13 13 12 12 9 9
+9 9 9 7 7 7 7 7 7 7 8 7 7 7 6 9 11 9 8 8 9 9 9 7 9 10 14 12 9 9 8 8 6
+6 6 6 8 6 6 8 6 6 6 6 6 9 8 10 10 9 10 10 13 10 11 6 7 11 6 6 8 6 6 6
+6 6 6 9 9 7 7 8 8 8 8 8 9 8 9 13 11 8 6 6 6 9 9 8 8 8 8 8 8 8 11 8 7
+10 12 7 7 9 8 6 6 8 8 7 7 10 8 8 7 7 7 8 8 8 8 7 7 7 7 7 10 9 10 12 10
+7 7 6 9 6 6 6 6 7 9 7 7 4 10 9 7 6 8 6 6 8 6 6 9 6 7 7 10 10 10 10 13
+13 8 9 8 8 8 6 7 10 11 10 9 8 6 6 6 6 6 8 8 7 9 7 7 7 7 7 7 7 10 10 10
+8 8 8 7 7 8 8 8 8 6 6 7 7 7 9 8 6 8 6 6 6 8 8 8 7 9 9 12 15 9 8 7 9 8
+8 9 8 8 6 8 12 11 7 7 7 7 7 7 9 11 11 9 9 9 10 9 9 9 8 8 8 8 8 7 7 12
+13 13 15 11 11 7 7 7 9 11 10 12 6 6 6 6 8 7 7 7 8 10 10 10 10 13 9 12
+11 11 6 6 6 6 8 8 6 7 7 12 10 13 9 8 7 7 10 7 7 15 17 15 12 17 20 15
+13 15 6 6 6 6 8 8 6 6 6 7 7 7 10 7 7 10 11 8 8 5 5 8 8 12 12 8 10 6 6
+6 6 8 8 7 7 7 12 10 7 7 9 7 7 9 9 8 9 10 10 8 8 9 11 9 8 9 9 11 11 9 9
+8 6 8 6 6 6 6 6 6 6 6 8 8 8 8 12 6 6 6 6 8 6 7 7 6 8 6 6 8 8 10 11 10
+12 7 8 8 9 12 6 6 6 6 6 6 6 6 8 9 9 9 9 9 8 7 8 7 7 7 8 7 7 7 7 9 9 9
+9 6 6 6 6 14 8 8 8 8 8 6 6 7 7 7 11 9 7 6 6 6 6 10 6 6 6 8 8 7 8 7 7 6
+7 7 8 8 12 13 15 15 21 9 10 9 8 8 10 6 6 6 6 4 4 4 8 8 8 7 6 6 6 6 6 6
+6 6 6 6 6 9 9 9 6 6 6 10 6 10 15 20 12 9 8 6 6 6 6 6 8 8 6 6 6 6 8 12
+13 12 10 9 8 11 8 10 9 7 7 4 4 6 6 8 4 4 4 6 8 6 6 6 8 8 13 10 8 9 9 9
+7 7 9 10 8 8 6 6 6 6 6 6 10 11 10 10 10 7 7 8 6 6 8 6 6 8 10 13 8 11 8
+11 10 6 6 6 7 11 14 11 11 6 6 8 8 8 9 9 9 9 7 7 10 10 6 6 6 6 6 6 8 8
+8 7 7 7 8 8 11 11 11 7 6 8 6 6 8 8 6 6 10 10 13 8 8 6 6 6 8 9 9 6 6 9
+9 8 8 6 6 8 6 6 8 8 4
+
+
+Sequence : 22ak93c1.r1ta
+Is_read
+Padded
+Staden_id 12
+Clipping QUAL 31 335
+Align_to_SCF 1 1315 1 1315
+SCF_File 22ak93c1.r1taSCF
+Primer Universal_primer
+Strand Reverse
+Dye Dye_terminator
+Template 22ak93c1
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 30 "M13mp18"
+Tag ALUS 31 172
+Tag DONE 150 150 "AUTO-EDIT: replaced T by a at 150 (terminator, isolated, strong)"
+Tag DONE 178 178 "AUTO-EDIT: replaced G by t at 178 (terminator, isolated, strong)"
+Tag DONE 188 188 "AUTO-EDIT: replaced T by a at 188 (terminator, isolated, strong)"
+Tag DONE 230 230 "AUTO-EDIT: replaced T by g at 230 (terminator, isolated, strong)"
+Tag DONE 261 261 "AUTO-EDIT: replaced C by g at 261 (terminated, isolated, strong)"
+Tag ALUS 262 446
+Tag DONE 305 305 "AUTO-EDIT: replaced T by g at 305 (terminated, isolated, strong)"
+Tag DONE 310 310 "AUTO-EDIT: inserted g at 310  (terminated, compound, strong)"
+Tag DONE 325 325 "AUTO-EDIT: replaced A by g at 325 (terminated, isolated, strong)"
+
+DNA : 22am91h5.r1t
+GGGnnATAGATACGATCCGAGCTCGGTACCCATGTTGGCCAAGCTGGTCTCGAGCTCCTG
+ACCTAGGATTACAGGCCTAAGCCACCGCACCCGGCATGATGGGTCTTTATTCTTCAAAGC
+AGGAGGAAGGGATCCTAGAAAAACAGAGACAAGGCCAAACATGGTAGCTCACACCTGTAA
+TCCCAGCACTTTGGGAGGCCAGTGCGGGTGAATCACGAAGTCAGGAGTTCAAGACCAGCC
+TGGCCAACATGGTGAAACACCGTCTCTACTAAAATAAAAAGAAATTAGCTGGGTGTCGTG
+GCAGGTGCCTGTAATCCCAGCCACTTGGGAAGCTGAGGCAGGAGAATCGCTTGAACCCGG
+GAGGTGGAGGTTGCAnTGAGCCGAGATCACGCGACTGCACTCCAGCCCAACCAATAATGT
+GAGACTCTGTCTCGAnAAnnnAAAGCnGAAAACAGACnnCTAGTACnGTACTTAACGGGG
+TAATTATAATAATTAAATGAAAAAAATAnCTGTGAGGTGATTGATATAnTGCTGTTGCTA
+AATACAACTAACATTTTAATATACCGGTGGAGTGTTTCTAATCTGAnAATCCAAATTAGA
+AATGCTCACAGTCTGAAACTTTTTGAGCACCAACTAATGTTCAAAGAATTGCTATGGACC
+ATATGGGTGGTTGATTTT
+
+BaseQuality : 22am91h5.r1t
+4 8 4 4 4 4 4 7 10 9 10 15 10 7 7 10 10 9 19 20 33 34 34 34 36 36 33
+33 31 33 33 32 31 31 26 26 27 23 30 26 26 26 26 34 34 38 38 38 37 45
+45 37 37 37 37 45 45 45 45 37 37 37 45 45 32 18 10 21 18 28 29 33 33
+33 33 45 45 42 42 37 33 33 33 36 33 33 30 30 30 30 32 36 33 33 33 33
+33 33 37 37 45 45 37 37 37 33 33 35 35 35 35 43 35 42 42 35 35 34 34
+34 29 29 22 27 27 27 37 27 37 45 33 30 30 37 28 32 32 10 10 21 32 32
+34 27 23 12 11 9 9 9 18 21 28 31 33 30 30 30 30 34 34 34 34 31 33 34
+34 33 31 33 33 33 33 33 33 45 45 40 37 37 37 33 33 30 30 30 30 33 30
+33 26 26 26 25 23 18 14 12 20 23 27 25 21 21 21 28 28 30 31 31 31 31
+32 21 21 12 6 6 9 9 14 17 19 17 10 16 13 8 8 11 13 9 13 12 14 14 21 23
+26 34 36 34 31 22 24 20 28 28 33 27 28 25 27 24 34 32 23 12 12 12 23
+23 33 28 34 34 37 32 32 15 11 11 32 32 40 37 38 18 11 9 9 18 25 45 34
+28 28 9 9 20 29 45 38 34 23 23 10 23 23 33 33 33 21 21 13 25 25 25 29
+27 33 30 33 33 34 33 30 30 19 19 30 30 30 26 26 30 27 27 23 19 15 24
+27 28 21 9 9 9 19 14 12 10 12 12 13 17 28 18 19 21 24 24 25 24 24 24
+24 18 18 13 18 8 8 8 10 10 10 7 7 7 8 8 12 15 4 4 4 25 18 21 17 15 16
+14 9 9 17 14 17 9 17 8 8 10 10 10 11 17 17 22 24 23 24 24 19 13 10 8
+15 15 10 9 9 9 16 12 12 14 19 18 26 24 22 22 24 25 21 24 24 32 19 20 9
+9 4 4 4 4 4 4 4 4 17 16 16 4 4 4 7 10 8 7 6 13 8 8 4 4 4 4 8 8 7 7 7 4
+4 4 17 23 12 9 8 7 7 8 7 10 10 8 6 6 6 6 6 10 10 7 7 7 7 9 7 8 10 7 7
+8 6 6 8 8 8 9 10 7 12 4 4 4 9 9 7 6 6 7 6 6 10 9 7 8 10 7 7 7 9 4 4 4
+14 8 8 9 7 6 6 8 8 8 7 7 6 6 9 7 7 10 13 8 9 8 8 8 14 7 7 7 7 9 8 12
+12 4 4 4 7 7 7 7 8 8 8 7 7 7 7 7 7 8 10 8 6 6 6 4 4 4 8 12 9 9 17 9 7
+7 7 7 6 6 6 6 8 10 6 6 6 8 6 6 6 6 6 6 6 6 6 6 6 6 6 13 11 9 8 7 8 7 7
+7 8 7 9 8 7 6 6 4 6 6 8 7 7 7 6 8 6 6 8 7 7 9 9 10 11 7 6 6 6 6 6 12 9
+8 8 12 11 10 8 7 7 4 4 4 7 7 8 4
+ 
+
+Sequence : 22am91h5.r1t
+Is_read
+Padded
+Staden_id 20
+Clipping QUAL 32 361
+Align_to_SCF 1 678 1 678
+SCF_File 22am91h5.r1tSCF
+Primer Universal_primer
+Strand Reverse
+Dye Dye_terminator
+Template 22am91h5
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 31 "M13mp18"
+Tag ALUS 153 443
+
+DNA : 22aq40a8.s1
+AAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCACAGAGACAAGGCCAAACATGG
+TAGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAGTGCGGGTGAATCACGAGGTCAG
+GAGTTCAAGACCAGCCTGGCCAACATGGTGAA-CACCGTCTCTACTAAAATAAAAAGAAA
+TTAGCTGGGTGTCGTGGCAGGTGCTtGTAATCCCAGCCACTTGGGAAGCTGAGGCAGGAG
+AATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCGACTGCACTCCA
+GCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGACAAGACAACTAG
+TACAGTACTTACAGGGTTATTATGATGATTAnATGAGAGAATAGCTGTGAGGTGATTGAT
+ATAGTGCTGTGCTTAAATACAACTATCAATTTTATTATACGGGGTTGAGTGTTTCCAATC
+TGAAAATCCCAAATTTGGAATGCTCTACGGTCTGAAACCTTTTTTGAGCACCGACCTAAA
+
+BaseQuality : 22aq40a8.s1
+7 9 10 11 11 23 27 25 25 9 9 9 12 14 23 27 24 21 21 16 22 22 22 24 23
+23 23 30 30 30 30 30 32 34 31 31 34 34 28 28 28 30 30 30 28 28 28 34
+34 34 34 34 34 37 34 34 34 34 34 34 37 37 37 37 37 37 37 32 34 29 29
+29 45 38 40 40 37 37 32 37 37 37 37 37 37 37 37 37 40 40 40 40 40 37
+30 30 32 22 22 22 32 32 41 45 45 41 41 45 30 32 27 32 32 40 37 37 40
+37 37 45 45 45 40 40 37 37 37 37 37 37 37 37 40 37 37 37 31 32 28 28
+28 32 32 37 34 37 38 38 38 27 27 11 1 11 10 21 27 34 34 37 37 34 34 45
+45 45 38 37 37 37 37 37 37 34 34 34 34 33 30 30 28 28 28 28 28 30 37
+37 37 37 33 36 36 31 31 31 24 24 24 24 21 21 17 12 4 4 4 14 17 26 25
+25 31 34 34 31 31 31 33 31 31 34 35 37 37 32 32 34 34 30 31 31 34 30
+33 30 30 30 33 33 30 30 23 22 22 32 32 40 34 36 34 31 26 28 26 24 16 9
+9 9 8 15 22 22 25 25 31 31 30 30 28 30 30 30 30 28 28 28 30 30 30 30
+30 37 32 27 23 18 18 27 27 34 34 31 33 30 30 28 28 28 28 28 30 34 33
+33 33 33 30 26 26 23 23 23 30 37 36 32 32 24 23 21 20 28 31 29 30 22
+21 18 13 15 23 26 34 34 36 33 30 30 30 30 25 25 25 26 23 26 25 24 24
+25 27 25 22 14 15 12 12 12 21 21 25 25 22 21 21 24 19 19 14 16 11 13
+14 20 27 30 28 28 28 30 33 34 34 23 23 16 7 7 4 4 4 18 17 21 23 21 17
+11 13 18 18 18 20 18 18 11 14 7 7 7 15 20 18 11 10 15 23 18 16 10 8 8
+13 17 18 14 14 14 13 13 14 17 18 13 13 9 9 9 14 12 18 18 11 11 5 5 5 6
+6 6 8 12 10 13 15 11 11 11 8 6 6 6 6 6 8 6 6 8 8 8 7 7 9 9 8 8 19 8 8
+14 8 8 6 6 11 9 9 8 14 6 6 7 7 8 6 7 6 6 9 9 10 15 14 12 7 7 7 7 13 15
+22 22 19 14 9 8 8 8 12 15 19 7 6 10 9 8 6 7 9 9 10 10 8 8 7 7 4 4
+
+
+Sequence : 22aq40a8.s1
+Is_read
+Padded
+Staden_id 3
+Clipping QUAL 39 388
+Align_to_SCF 1 152 1 152
+Align_to_SCF 154 540 153 539
+SCF_File 22aq40a8.s1SCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_primer
+Template 22aq40a8
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 38 "M13mp18"
+Tag ALUS 49 339
+Tag DONE 206 206 "AUTO-EDIT: replaced N by t at 206 (terminated, compound, strong)"
+
+DNA : 22an02b10.s1t
+CGTGCCTGCAGGTCGACTCTAGAGGATCCCCCCTGTAATCCCAGCACTTTGGGAGGCCAg
+TGCGGGTGAATCACgATGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACACCG
+TCTCTACTAAAATAAAAAGAAATTAGCTGGGTGTCgTGGCAGGTGCCTGTAATCCCAGCC
+ACTTGGGAAGCTGAgGCAGGAGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCC
+GAgATCACGCGACTGCACTCCAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAA
+AAAGCAGAGACAAGACAACTAGTACAGTACTTACaGGGTTATTATGATGATTAAATGAGA
+gAATAGCTGTGAgGTGATTGATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATA
+CGGGTTGAGTGTATCTAATCTGAAAATCCAAAATCAGAAATGCGCTACAGTCTGAAACTT
+TTTTGAGCACCGACCTAATGTTCCAAGGAAGTGCTTATCGGAGCACCATGGGTCGGTACA
+CATTTGGGTCGGGAATATnCCACCAGTGAGTACCATAAAATGCCAATATCCCCTCCCACC
+CCCAACA
+
+BaseQuality : 22an02b10.s1t
+7 7 9 12 10 15 16 17 13 13 21 18 23 17 28 28 37 37 37 33 26 26 17 19
+12 21 25 36 45 45 34 34 34 34 34 37 37 37 37 37 32 25 9 9 13 17 17 14
+18 18 21 29 32 19 18 18 18 21 9 9 9 23 19 30 30 37 29 37 37 32 34 23
+20 13 13 10 13 22 32 21 17 17 23 18 10 10 10 12 13 9 9 14 23 29 28 22
+22 32 33 37 34 34 34 34 34 34 34 30 37 32 37 37 41 34 33 33 33 21 21
+14 14 14 24 28 41 37 32 32 21 21 21 25 30 30 37 30 37 30 30 30 32 36
+28 27 17 27 27 37 32 37 36 37 25 23 10 10 10 32 21 23 10 15 10 23 23
+36 32 32 34 34 34 37 37 25 25 13 9 9 21 25 25 32 32 32 41 32 28 28 11
+22 22 32 32 13 13 10 11 9 10 10 12 12 14 32 32 29 22 22 21 21 17 21 27
+41 41 41 28 27 32 27 27 29 37 32 34 17 15 14 28 23 25 20 9 9 9 25 10
+12 12 14 13 10 10 9 16 13 23 23 14 14 22 25 34 28 28 22 28 28 28 19 18
+10 10 11 19 21 28 28 28 30 30 30 23 18 10 10 28 32 32 34 28 28 28 28
+28 31 34 34 25 25 12 19 11 14 14 25 21 24 24 18 18 18 22 23 13 11 10
+10 10 10 14 13 10 9 9 7 8 14 17 16 10 10 13 16 15 18 18 18 18 14 15 12
+12 9 12 9 12 12 13 9 14 10 18 19 14 9 13 11 18 14 15 15 16 20 19 18 14
+9 8 8 6 6 6 11 9 9 14 17 20 12 13 6 6 8 8 11 10 21 23 15 14 13 13 14
+21 27 28 32 29 34 28 27 23 14 10 10 12 17 15 13 11 11 11 18 28 33 24
+18 14 12 17 12 12 18 22 19 19 24 28 20 21 19 19 20 17 17 26 25 20 9 14
+7 7 7 15 21 33 27 27 18 20 19 15 15 20 18 14 14 8 8 9 9 9 7 7 7 7 7 8
+9 9 12 7 7 9 11 10 11 13 13 11 7 7 8 15 17 26 25 25 27 25 24 21 19 20
+18 13 13 14 9 9 14 18 17 12 15 16 21 20 18 8 7 6 6 6 13 20 25 18 11 14
+9 9 9 11 11 12 12 15 15 18 10 10 8 8 10 10 9 9 9 16 11 7 7 6 6 8 4 4 4
+4 4 6 10 10 13 13 12 6 6 6 6 12 15 12 11 11 4 4 4 6 6 6 6 6 8 6 6 6 8
+6 8 11 13 8 8 6 6 6 6 9 16 9 13 13 17 14 13 9 7 7 12 8 8 8 9 9 9 9 9 9
+8 8 8 10 9 7
+
+
+Sequence : 22an02b10.s1t
+Is_read
+Padded
+Staden_id 21
+Clipping QUAL 32 446
+Align_to_SCF 1 607 1 607
+SCF_File 22an02b10.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22an02b10
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 31 "M13mp18"
+Tag ALUS 32 301
+Tag DONE 60 60 "AUTO-EDIT: inserted g at 60  (terminated, compound, strong)"
+Tag DONE 75 75 "AUTO-EDIT: replaced A by g at 75 (terminated, isolated, strong)"
+Tag DONE 156 156 "AUTO-EDIT: replaced C by g at 156 (terminated, isolated, strong)"
+Tag DONE 195 195 "AUTO-EDIT: replaced A by g at 195 (terminated, isolated, strong)"
+Tag DONE 243 243 "AUTO-EDIT: replaced T by g at 243 (terminated, isolated, strong)"
+Tag DONE 335 335 "AUTO-EDIT: replaced T by a at 335 (all_stranded, isolated, strong)"
+Tag DONE 361 361 "AUTO-EDIT: replaced C by g at 361 (terminated, isolated, strong)"
+Tag DONE 373 373 "AUTO-EDIT: replaced C by g at 373 (terminated, isolated, strong)"
+
+DNA : 22ak67d6.r1t
+CGCGCCCCTCnGACTCCGGCCCTAnCACGGTACCCACTTTGGGAGGCCAGTGCGGGTGAA
+TCACGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACACCGTCTCTACTAA
+AATAAAAAGAAATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAGCCACTTGGGAAG
+CTGAgGCAGGAGAATCGCTTGAACCCGGGAgGTGGAGGTTGCAGTGAGCCGAGATCACGC
+GACTGCACTCCAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGA
+TCAGATCACTAGTACAGTACTTACGGGCTTATTATGATGATTAAATGAGAGAATACCTGT
+GAGGTGATTGATATATTGCTGTGCTTAATACAnACTATCATTTTATTATACGGGTCGAGT
+GTTTCTAATCTGACAATCCAAATTAGAAATGCTCTACAGTCTGAAACCTTTTTGAGCACC
+CACCTACTGTTCAAAGGACGTGCTTATTGGAGCATTATGGGTTGTAACATTTTTGGGTTG
+GGAATTTTCACCCATTAGTACTATAAATGCCATTTTCCAAAAAATCTGAATCTAAAATTT
+CTGGTCCTACCAGCATTTGCTAGGGATCCCACCCGTCTTCTTCCTTACCTGTTTAAGTCT
+TATAAATC
+
+BaseQuality : 22ak67d6.r1t
+6 6 6 4 8 11 15 8 4 4 4 4 8 6 6 9 7 8 9 8 4 4 4 4 4 4 10 9 9 21 19 33
+30 33 30 30 30 28 28 28 23 23 20 11 11 16 12 13 17 21 25 28 33 33 33
+38 34 34 34 33 30 22 22 12 14 21 10 12 21 21 16 14 11 13 13 14 21 21
+21 19 15 18 23 23 25 30 28 33 38 38 33 33 30 30 30 30 30 30 30 37 37
+45 45 33 33 33 33 33 33 40 42 49 42 40 40 40 34 34 31 35 34 35 37 42
+42 37 32 19 9 9 11 23 19 28 30 30 28 38 45 37 37 37 32 32 24 34 34 30
+30 26 21 20 11 27 27 40 34 30 30 28 30 30 30 23 18 17 9 9 18 16 16 24
+24 24 23 21 18 11 11 18 25 25 16 9 7 9 15 11 8 7 9 7 7 8 16 16 27 28
+25 20 16 10 10 9 13 8 8 7 12 4 4 4 14 6 6 6 7 9 6 7 7 7 7 16 10 20 15
+13 11 9 9 8 8 8 15 8 10 10 17 14 9 9 19 15 15 7 7 12 20 19 25 25 22 18
+17 7 9 12 20 21 25 21 21 20 22 28 32 30 25 25 12 12 18 32 32 36 49 49
+33 33 24 20 13 12 16 18 20 14 25 23 25 19 12 11 8 8 4 4 4 4 4 8 8 6 4
+6 8 15 17 21 19 15 8 8 8 8 10 7 7 13 9 7 11 14 6 6 4 4 4 6 12 8 13 17
+21 18 19 19 20 18 18 15 10 15 14 13 14 8 8 8 9 8 8 10 10 8 8 15 17 17
+17 17 14 13 19 17 16 12 10 14 15 17 15 14 8 8 7 7 15 19 18 12 11 16 12
+15 11 10 13 13 11 4 4 4 10 12 12 18 12 14 9 10 9 10 21 22 18 14 6 6 6
+6 6 8 4 4 4 8 8 7 8 7 9 14 7 8 7 10 16 31 17 14 4 4 4 10 11 8 10 13 17
+13 8 8 9 8 8 12 12 14 14 7 7 7 7 7 7 7 8 9 13 11 10 7 10 7 6 6 10 8 8
+23 23 12 7 7 7 7 7 10 10 18 6 6 6 6 6 6 7 7 7 6 6 6 7 6 6 4 4 4 4 8 11
+18 15 23 11 9 8 7 7 7 7 7 7 8 15 18 8 10 10 12 8 6 8 6 6 6 7 7 12 10
+12 18 11 12 10 19 19 15 15 10 10 9 7 11 7 8 9 10 7 7 6 6 6 6 4 6 7 7 7
+7 9 9 9 13 13 9 7 7 7 9 7 7 7 6 6 6 6 8 8 9 8 11 8 7 6 6 6 6 9 7 8 7 7
+6 8 6 6 9 10 12 10 13 12 6 6 6 6 6 7 6 6 7 7 7 11 13 11 9 8 6 6 8 6 6
+8 12 14 8 8 8 6 13 8 8 8 6 7 8 7 8 9 8 10 6 6 10 11 17 20 16 11 11 7 6
+8 8 9 8 6 12 10 7 7 8 8 4
+
+
+Sequence : 22ak67d6.r1t
+Is_read
+Padded
+Staden_id 19
+Clipping QUAL 30 292
+Align_to_SCF 1 668 1 668
+SCF_File 22ak67d6.r1tSCF
+Primer Universal_primer
+Strand Reverse
+Dye Dye_terminator
+Template 22ak67d6
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Tag ALUS 35 291
+Tag DONE 185 185 "AUTO-EDIT: replaced C by g at 185 (terminated, isolated, strong)"
+Tag DONE 211 211 "AUTO-EDIT: replaced T by g at 211 (terminated, isolated, strong)"
+
+DNA : 22ak64b1.s1t
+TGGGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAACACCGTCTCTACTAAA
+ATAAAAAGAAATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAGCCACTTGGGAAGC
+TGAGGCAGGAGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAgATCACGCG
+ACTGCACTCCAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGAC
+AAGAcCACTAGTACAGTACTTACAGGGTtATTATGATGATTAAATGAGAGAATAGCTGTG
+AGGTGATTGATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATACGGGTTGAGTG
+TTTCTAATCTGAaAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACC
+GACCTAAATGTTCAAAGGGAGGTGCTTAATTGGAGCATTATGGGTTGTTAGATCTTTGGG
+TTGGGAATATTCAACC
+
+BaseQuality : 22ak64b1.s1t
+4 4 8 11 13 20 16 16 16 19 24 32 31 37 37 35 34 29 32 18 32 24 33 33
+37 37 37 23 32 11 10 21 17 37 34 34 45 45 45 45 32 29 26 26 28 30 30
+33 45 45 49 49 45 43 45 45 45 45 45 37 45 45 45 45 45 45 45 37 37 37
+38 32 40 37 41 37 45 32 45 45 44 37 45 30 45 45 33 33 33 33 33 22 37
+37 45 45 45 40 37 34 37 37 33 32 32 33 33 33 33 40 40 40 42 40 42 37
+37 37 45 45 45 45 45 35 34 34 28 28 18 21 17 17 27 38 38 45 34 34 34
+34 34 34 34 34 34 34 34 34 34 34 34 37 34 34 33 33 33 33 28 31 31 36
+34 31 36 36 34 33 20 14 8 8 8 8 14 23 27 31 36 33 36 38 30 30 28 28 28
+30 30 27 30 30 30 30 33 33 23 21 18 18 17 20 32 37 37 45 45 45 45 34
+34 34 45 45 45 44 45 37 45 45 45 37 40 37 37 33 40 44 40 40 36 31 31
+28 25 22 27 14 14 8 9 10 8 7 8 7 12 18 18 23 15 9 9 15 15 20 20 25 28
+25 21 21 18 18 8 9 8 6 6 8 11 14 28 28 28 30 30 30 30 31 30 31 25 22
+22 27 15 15 15 16 13 17 25 25 30 25 27 28 31 24 24 19 24 29 33 27 31
+31 31 31 28 27 22 14 15 27 32 40 37 36 36 36 33 33 34 33 27 13 14 7 7
+8 18 23 32 32 23 20 20 33 30 27 27 21 14 14 18 21 18 21 15 15 17 11 11
+16 24 23 30 23 14 10 13 14 15 10 8 9 16 16 28 4 4 4 10 11 12 9 8 10 14
+20 18 19 19 19 10 10 12 15 25 23 26 26 28 18 14 14 16 20 15 14 12 12
+12 21 23 25 24 21 17 17 14 21 20 19 12 9 9 8 9 8 10 7 6 9 8 7 8 9 7 7
+7 6 6 8 7 8 6 6 6 6 6 13 11 6 6 6 8 10 11 8 9 9 10 10 15 10 11 12 16
+13 10 9 7 7 7 7 9 10 8 8 7 6 6 6 6 6 14 10 7 7 7 9 9 10 11 16 16 7 6 4
+4 4 8 4
+
+
+Sequence : 22ak64b1.s1t
+Is_read
+Padded
+Staden_id 8
+Clipping QUAL 40 416
+Align_to_SCF 1 496 1 496
+SCF_File 22ak64b1.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak64b1
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 39 "M13mp"
+Tag CONF 44 44 "AUTOEDIT: possible compression"
+Tag CONF 96 96 "AUTOEDIT: possible compression"
+Tag DONE 172 172 "AUTO-EDIT: replaced A by g at 172 (terminated, isolated, strong)"
+Tag DONE 245 245 "AUTO-EDIT: replaced A by c at 245 (all_stranded, compound, strong)"
+Tag AMBG 246 246 "AUTOEDIT: Check this edit stack (conflict & possible misassembly))!"
+Tag DONE 269 269 "AUTO-EDIT: replaced C by t at 269 (all_stranded, isolated, strong)"
+Tag DONE 373 373 "AUTO-EDIT: replaced N by a at 373 (terminated, isolated, strong)"
+
+DNA : 22ak81h10.s1t
+GTGGCATGCCTGCAGGTCAACTCTAAAAGATCCCCTGAAACACCGTCTCTACTAAAATAA
+AAAGAAATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAgCCACTTGGGAAGCTGAG
+GCAGGAGAATCGCTTGAACCCGGGAgGTGGAgGTTGCAGTGAGCCGAGATCACGCGACTG
+CACTCCAGCCCAAcCAATAGTGTGAGACTCTGTCTCGAaaAAaAAAAAGCAgAGACaAGA
+CCACTAGTACAGTACTTACAGGGTTATTATGATGATTAAaTGAGAGAATAGCTGTGAGGT
+GATTGATATAGTGCTGTGCTTAATACaAACTATCATTTTATTATACGGGTTGAGTGTTTC
+TAATCTGAAAATCCaaAATTAGAAATGCTCTACAGTCTGAAACTTTtTTGAGCACCGACC
+TACTGTGCACAGGAAGTGCTTATTGGAGCATGATGGGTTGTTACATATTTGGGTTGGGAA
+TATTCACCCAGTAAGTACTATAAAATGCAAATATTCCAACAAG
+
+BaseQuality : 22ak81h10.s1t
+6 6 6 6 6 8 9 16 11 13 21 20 22 22 22 15 14 9 9 9 9 15 18 15 9 9 9 9 9
+15 23 36 36 36 36 34 38 40 30 28 28 28 30 32 37 37 36 38 37 44 45 45
+49 49 45 45 37 37 38 45 45 45 38 37 37 38 38 37 37 34 34 34 32 32 38
+45 49 45 45 45 45 37 33 33 27 27 18 18 30 32 36 36 36 36 36 36 36 34
+32 21 18 4 4 4 18 17 27 28 33 36 36 36 49 36 36 36 34 34 45 27 27 30
+25 27 12 20 20 34 34 38 37 37 34 34 25 25 18 18 21 29 38 45 21 28 14
+14 14 27 21 34 18 17 17 32 27 25 22 13 13 10 18 15 32 32 32 37 28 22
+22 21 14 10 10 11 23 22 28 20 14 14 8 8 11 20 20 16 12 16 16 28 21 19
+10 10 10 19 18 20 20 14 14 32 32 34 32 25 18 18 18 18 27 32 32 24 24
+23 23 14 10 10 10 9 9 10 11 12 12 12 12 17 11 11 11 10 8 6 6 8 8 10 16
+16 16 11 14 10 9 9 9 17 11 11 11 14 15 22 18 14 14 11 11 15 27 22 19
+23 27 30 28 22 18 16 11 10 10 11 9 10 11 11 12 15 20 27 27 23 21 14 10
+10 10 10 14 22 25 22 14 14 22 32 38 34 34 34 31 24 18 14 12 9 10 10 23
+18 24 16 16 18 18 18 19 18 17 21 18 18 12 14 21 27 37 34 34 28 28 34
+27 27 28 31 20 18 11 9 9 12 11 11 27 15 15 20 23 26 14 8 8 8 8 14 16
+23 19 19 28 31 31 31 25 18 11 11 18 10 10 10 10 11 12 16 16 21 23 12
+12 13 23 13 13 19 11 10 10 10 10 10 18 21 28 16 19 29 32 19 19 8 8 8
+10 10 18 18 22 21 16 18 22 21 11 11 14 14 15 14 18 7 7 7 7 7 7 14 18
+10 10 9 9 14 15 15 23 20 22 19 19 21 16 11 11 9 8 8 9 9 18 14 11 9 9
+15 8 9 10 8 9 8 8 8 14 14 25 27 16 9 8 14 8 9 17 13 18 15 8 8 7 7 7 7
+6 6 6 6 6 4 4 4 8 9 10 17 11 15 12 17 9 9 10 7 7 7 10 11 10 7 7 7 6 6
+8 6 6 8 4
+
+
+Sequence : 22ak81h10.s1t
+Is_read
+Padded
+Staden_id 5
+Clipping QUAL 36 418
+Align_to_SCF 1 523 1 523
+SCF_File 22ak81h10.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak81h10
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 35 "M13mp1"
+Tag ALUS 36 216
+Tag DONE 103 103 "AUTO-EDIT: replaced N by g at 103 (terminated, isolated, strong)"
+Tag DONE 146 146 "AUTO-EDIT: replaced T by g at 146 (terminated, isolated, strong)"
+Tag DONE 152 152 "AUTO-EDIT: replaced A by g at 152 (terminated, isolated, strong)"
+Tag DONE 194 194 "AUTO-EDIT: replaced G by c at 194 (all_stranded, isolated, strong)"
+Tag DONE 219 219 "AUTO-EDIT: replaced G by a at 219 (all_stranded, compound, strong)"
+Tag DONE 220 220 "AUTO-EDIT: replaced C by a at 220 (all_stranded, compound, strong)"
+Tag DONE 223 223 "AUTO-EDIT: replaced G by a at 223 (all_stranded, isolated, strong)"
+Tag DONE 232 232 "AUTO-EDIT: replaced C by g at 232 (all_stranded, isolated, strong)"
+Tag DONE 237 237 "AUTO-EDIT: replaced G by a at 237 (all_stranded, isolated, strong)"
+Tag DONE 280 280 "AUTO-EDIT: replaced G by a at 280 (terminated, isolated, strong)"
+Tag DONE 327 327 "AUTO-EDIT: replaced G by a at 327 (terminated, isolated, strong)"
+Tag DONE 375 375 "AUTO-EDIT: replaced T by a at 375 (terminated, compound, strong)"
+Tag DONE 376 376 "AUTO-EDIT: replaced C by a at 376 (terminated, compound, strong)"
+Tag DONE 407 407 "AUTO-EDIT: replaced C by t at 407 (terminated, isolated, strong)"
+
+DNA : 22ak66c6.s1t
+nCGTGGCTGCCTGCAGGTCGACTCTAGAGGATCCCCACCGTCTCTACTAAAATAAAAAGA
+AATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAGCCACTTGGGAAGCTGAGGCAGG
+AGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCGACTGCACTC
+CAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGACAAGACAACT
+AGTACAGTACTTACAGGGTTATTATGATGATTAAATGAGAGAATAGCTGTGAGGTGATTG
+ATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATACGGGTTGAGTGTTTCTAATC
+TGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAATG
+TTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGGGTTGGGAATATTC
+AACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAATCTGAAACATtTC
+TGGTCCTAAGCAAGCATnTTGCAAAGGGATACGCAACCTGTAGTACGTCCTTTATCATTG
+TnTCAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAnCAnATTCGATTGTA
+TTATT
+
+BaseQuality : 22ak66c6.s1t
+4 4 8 4 6 10 13 21 24 25 33 33 33 30 27 21 15 27 19 30 30 33 33 30 21
+21 10 9 17 11 27 27 37 38 33 35 35 35 35 44 45 45 38 37 37 45 45 45 43
+43 43 45 45 45 45 45 45 45 21 21 23 30 30 34 37 37 38 45 32 45 37 41
+37 45 30 45 45 34 34 34 32 29 22 32 32 45 45 45 33 28 28 37 37 34 34
+34 35 34 34 34 34 34 34 37 37 40 41 37 34 37 37 37 32 24 22 27 29 25
+27 20 21 21 21 27 45 41 40 40 40 42 34 34 37 41 45 51 45 45 37 30 37
+41 41 37 36 28 30 30 30 22 33 33 35 33 33 41 51 51 45 39 39 39 30 28
+33 34 34 39 34 41 34 34 41 33 33 39 39 39 33 33 33 30 33 33 33 30 29
+19 19 24 25 32 33 45 36 36 36 36 41 41 30 30 30 37 37 43 51 51 51 51
+45 37 37 30 37 37 37 51 51 51 51 51 37 37 28 28 10 10 10 13 10 10 10 9
+9 9 21 21 28 37 37 37 33 33 33 33 33 33 34 34 34 33 33 33 33 21 25 25
+22 22 29 29 33 33 33 33 31 31 27 28 17 23 23 28 26 24 32 10 10 10 15
+15 26 32 32 37 45 45 32 45 33 26 24 32 37 37 35 34 37 35 37 34 37 37
+40 37 37 45 45 38 38 45 45 40 37 37 37 34 30 30 28 28 28 30 37 37 34
+34 34 34 34 33 31 31 25 21 19 22 21 25 30 32 32 30 22 22 25 28 24 25
+26 17 17 23 27 27 33 31 31 34 38 38 38 38 34 26 17 15 8 8 11 16 25 31
+32 33 29 26 32 29 33 34 34 34 36 33 34 34 31 27 31 34 38 45 45 36 37
+37 37 36 36 38 34 32 28 25 22 22 21 17 18 29 29 23 23 22 27 25 20 18
+11 11 15 15 16 23 21 17 19 24 24 24 31 31 31 33 33 33 31 33 33 29 29
+29 29 29 31 31 31 23 24 19 12 10 16 10 10 20 10 10 13 15 30 23 29 23
+28 18 10 10 16 21 18 19 19 24 24 12 11 9 9 10 21 23 31 31 33 28 28 14
+17 17 28 21 30 24 30 28 22 26 23 19 10 10 12 23 22 23 19 12 10 9 9 10
+19 24 29 30 34 34 34 34 34 29 25 25 32 26 31 31 32 20 17 15 12 12 4 4
+4 17 23 33 30 24 18 13 18 16 12 16 8 9 9 19 15 15 4 4 4 7 11 6 6 6 7 8
+9 17 12 13 19 23 25 13 13 13 19 21 29 32 30 26 20 20 12 9 9 8 9 8 8 15
+24 17 15 8 8 9 17 16 4 4 4 8 8 13 15 23 14 9 8 8 10 10 17 23 21 17 12
+13 22 20 19 15 11 11 9 8 8 8 8 8 8 10 9 9 7 8 8 10 12 10 10 12 4 4 4 4
+4 4 10 10 10 10 10 9 8 8 7 8 9 11 9 9 4
+
+
+Sequence : 22ak66c6.s1t
+Is_read
+Padded
+Staden_id 18
+Clipping QUAL 37 548
+Align_to_SCF 1 665 1 665
+SCF_File 22ak66c6.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak66c6
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 36 "M13mp"
+Tag ALUS 38 221
+Tag DONE 538 538 "AUTO-EDIT: replaced N by t at 538 (terminated, isolated, strong)"
+
+DNA : 22ak93c1.s1lt
+TTTCTTTGGGGGCAATCTTGATGCTAGCGTCGTTCAAGATGATCCACACCCAGCTCACTG
+TTTTATTCTCTCCAATAATCCTAAGCAAGCTCTCAAAATATAAATATGCTTTTTAATAAT
+ACAATCGAATTTGCTATACCACCTCAGAATCTGTACCACAATATATTAACTACTTAAAAC
+AATGATAAAGAACGTACTACAGGTTGCGTATCCCTTTGCAAAATGCTTGCTTAGGACCAG
+AAATGTTTCAGATTTCAGATTTTTTTTGGAATATTTGCATTTTATAGTACTTACTGGTTG
+AATATTCCCAACCCAAAAATCTAACAACCCATAATGCTCCAATAAGCACTTCCTTTGAAC
+ATTAGGTCGGTGCTCAAAAAAGTTTCAGACTGTAGAGCATTTCTAATTTTGGATTTTCAG
+ATTAGAAACACTCAACCCGTATAATAAAATGATAGTTTGTATTAAGCACAGCACTATATC
+AATCACCTCACAGCTATTCTCTCATTTAATCATCATAATAACCCTGTAAGTACTGTACTA
+gTTGTCTTGTCTCTGCTTTTTTTTTTTCgAgACAGAGTCTCACACTATTGGTTGGGCTGG
+AATGCAnTCGCGTGATCTCGGCTCACTGGCAACCTCCACCTCCCGGGG
+
+BaseQuality : 22ak93c1.s1lt
+4 4 4 4 4 4 4 4 9 6 4 8 15 11 4 6 4 12 8 9 10 12 11 8 8 6 7 8 14 14 9
+4 4 8 8 8 8 8 4 6 8 11 9 10 8 9 9 11 10 9 9 9 9 9 9 16 16 32 23 29 21
+27 27 22 22 20 28 32 37 37 34 31 30 28 28 24 29 29 34 34 38 32 32 18
+22 18 22 22 28 29 37 37 37 37 35 35 34 34 35 34 37 37 37 37 37 37 34
+37 37 37 37 32 32 28 28 31 30 30 30 23 23 23 28 27 21 32 13 13 16 24
+32 34 31 30 28 28 28 28 28 34 34 34 34 33 25 16 9 9 10 23 32 33 41 37
+34 28 30 30 30 28 28 22 27 25 32 25 30 27 22 24 22 22 28 25 24 30 30
+30 30 30 30 24 28 13 10 10 23 20 18 9 9 9 18 23 30 30 30 28 24 19 25
+11 11 12 27 31 34 30 30 30 30 37 45 51 41 39 28 28 28 25 25 24 30 34
+39 39 39 39 39 39 36 31 13 10 9 10 10 23 23 9 9 13 23 31 31 14 14 31
+31 13 9 9 9 23 23 23 13 9 9 9 31 31 41 51 36 31 14 13 31 19 30 31 31
+22 34 25 33 33 33 30 24 29 30 18 24 24 11 11 24 24 33 39 34 34 30 30
+34 22 22 14 19 18 23 23 17 31 24 37 33 33 33 33 33 28 34 18 18 27 34
+34 38 27 20 20 24 24 30 30 39 34 34 30 22 22 24 33 33 33 33 28 24 24
+22 30 22 24 24 33 33 33 39 51 51 41 32 27 17 25 17 31 31 34 19 15 10
+10 31 14 27 21 30 30 24 24 30 34 39 39 41 36 34 30 30 30 34 31 16 9 9
+10 20 19 16 11 9 9 9 13 23 22 28 28 37 33 33 33 28 32 18 13 10 20 18
+10 32 21 31 23 14 9 9 9 16 11 9 9 10 20 25 33 30 30 28 32 28 28 37 35
+35 34 34 34 34 34 37 40 37 33 32 25 12 10 10 16 16 28 32 32 34 30 28
+28 29 31 33 32 28 30 30 25 21 16 19 19 19 27 19 14 14 22 22 28 25 29
+29 29 30 30 23 23 21 18 14 21 17 24 19 32 32 37 37 31 30 30 28 31 30
+34 34 28 28 28 28 31 31 30 28 28 28 30 30 25 32 10 10 10 23 23 27 17
+17 22 17 23 23 23 24 20 17 4 4 4 14 16 24 22 19 18 13 19 9 12 14 25 25
+33 32 29 40 38 49 49 49 34 34 17 14 8 7 7 9 9 10 8 7 7 9 10 15 17 18
+22 24 18 18 18 22 20 24 24 28 18 19 18 19 21 24 25 14 12 8 8 9 14 12 4
+4 4 17 17 17 14 9 9 9 12 21 21 18 12 10 13 10 11 9 12 12 8 7 8 8 9 10
+9 15 10 8 9 10 10 21 21 28 27 18 14 7 7
+
+
+Sequence : 22ak93c1.s1lt
+Is_read
+Padded
+Staden_id 1
+Clipping QUAL 56 598
+Align_to_SCF 1 648 1 648
+SCF_File 22ak93c1.s1ltSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_primer
+Template 22ak93c1
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 47 "M13mp18"
+Tag DONE 541 541 "AUTO-EDIT: replaced N by g at 541 (all_stranded, isolated, strong)"
+Tag ALUS 559 647
+Tag DONE 569 569 "AUTO-EDIT: replaced A by g at 569 (all_stranded, isolated, strong)"
+Tag DONE 571 571 "AUTO-EDIT: replaced A by g at 571 (all_stranded, isolated, strong)"
+
+DNA : 22ak64e2.s1t
+GTAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTGCAAAATGCTTGCTTA
+GGACCAGAAATGTTTCAGATTTCAGATTTTTTTTGGAATATTTGCATTTTATAGTACTTA
+CTGGTTGAATATTCCCAACCCAAAAATCTAACAACCCATAATGCTCCAATAAGCACTTCC
+TTTGAACATTAGGTCGGTGCTCAAAAAAGTTTCAGACTGTAGAGCATTTCTAATTTTGGA
+TTTTCAGATTAGAAACACTCAACCCGTATAATAAAATGATAGTTTGTATTAAGCACAGCA
+CTATATCAATCACCTCACAGCTATTCTCTCATTTAATCATCATAATAACCCTGTAAGTAC
+TGTACTAGTTGTCTTGTCTCTGCTTTTTTTTTTTCGAGACAGAGTCTCACACTATTGGTT
+GGACTGGAGTGCAATTCnCCTGAATTCGGCTCACGGCAACTTCAACCTCCCGGGTTCAAG
+TGAATCTCCTC
+
+BaseQuality : 22ak64e2.s1t
+4 4 8 10 12 15 18 18 28 30 30 30 30 26 37 34 30 30 22 22 20 23 16 11
+11 18 23 23 23 28 9 9 10 9 21 12 32 34 45 45 45 45 38 30 30 30 30 30
+28 34 30 33 33 33 33 33 43 45 45 32 18 10 11 11 25 23 10 10 22 32 38
+37 27 27 27 23 20 9 9 10 23 25 25 21 9 9 10 29 32 45 45 45 45 45 45 37
+37 37 38 45 37 41 28 29 28 28 28 28 34 34 33 33 33 28 28 34 33 34 34
+34 34 34 34 45 37 37 29 37 37 45 45 45 45 42 37 34 35 35 35 34 34 34
+34 34 37 37 37 45 45 35 35 35 35 35 35 35 37 37 37 37 45 37 42 42 33
+30 30 33 33 33 40 34 34 34 34 34 34 40 40 40 37 37 37 34 34 34 34 34
+31 31 27 28 19 21 19 28 28 30 32 34 34 31 31 31 33 39 27 20 7 7 8 8 14
+15 11 11 17 28 23 28 17 9 9 11 23 27 33 33 40 40 40 40 40 40 40 37 32
+32 31 31 28 37 30 33 25 21 13 13 14 26 22 11 11 21 21 30 33 31 31 33
+37 37 37 45 49 45 40 40 40 40 40 45 40 40 40 40 40 31 29 29 32 27 37
+37 45 45 45 40 37 37 37 37 37 33 34 33 33 30 30 34 34 36 36 36 30 30
+28 28 28 27 38 38 36 31 33 32 27 20 20 20 28 32 33 24 24 29 36 34 30
+33 30 26 26 26 30 30 26 37 36 34 33 30 31 31 28 27 27 25 24 29 33 27
+27 31 31 31 31 33 36 38 38 32 32 21 13 13 16 19 19 24 25 33 30 33 33
+33 38 39 40 44 40 40 36 33 37 40 40 40 40 49 40 36 33 31 26 23 23 21
+18 20 14 12 11 14 17 25 31 33 33 33 36 30 30 30 30 30 28 28 20 15 14 4
+4 4 12 9 9 4 4 4 7 8 7 7 7 7 4 4 4 7 8 10 7 6 9 9 9 7 7 11 17 19 9 8 7
+7 8 7 8 9 7 6 10 6 7 6 6 7 7 8 8 8 12 13 8 8 8 8 10 10 9 8 7 6 6 8 13
+7 7 7 7
+
+
+Sequence : 22ak64e2.s1t
+Is_read
+Padded
+Staden_id 14
+Clipping QUAL 41 420
+Align_to_SCF 1 491 1 491
+SCF_File 22ak64e2.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak64e2
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 40 "M13mp"
+
+DNA : 22ak70f10.s1t
+TTGGCTGCCTGCAGGTCGACTCTAGAGGATCCCCCAGGGTTATTATGATGATTAAATGAG
+AGAATAGCTGTGAGGTGATTGATATAGTGCTGTGCTTAATACAAACTATCATTTTATTAT
+ACGGGTTGAGTGTTTCTAATCTGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACT
+TTTTTGAGCACCGACCTAATGTTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAG
+ATTTTTGGGTTGGGAATATTCAACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAA
+ATCTGAAATCTGAAACATTTCTGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCT
+GTAGTACGTTCTTTATCATTGTTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGT
+GGTATAGCAAATTCGATTGTATTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAG
+GATTATTGGAGAGAATAAAACAGTGAAGCTTTGGTGTTATGAGGGAATnTTAGATAGAAA
+AGTGCAGTnTTTCAGTTCATGCTCTnTCATTTnTTACTCCCTCCGGTTAAAGCTGAAGCT
+CAACAAAGATATAGTGATCTCTGTGGGCATTATAATCCTGTCCC
+
+BaseQuality : 22ak70f10.s1t
+4 4 6 8 8 9 19 23 33 33 34 33 27 21 18 27 18 27 27 34 34 31 27 23 13
+11 19 16 31 31 37 45 45 34 34 31 31 22 31 25 31 37 45 45 45 45 37 37
+37 37 37 37 45 37 37 37 45 38 29 27 27 32 32 37 45 33 37 37 45 37 45
+45 37 37 37 40 40 40 37 45 37 37 34 33 33 34 34 34 37 33 40 37 37 37
+33 33 33 33 33 33 33 33 33 32 33 41 41 35 35 35 40 40 36 36 36 34 33
+33 37 37 37 37 37 41 45 30 30 41 41 37 28 45 18 18 29 35 37 37 37 37
+41 41 51 51 51 51 51 33 33 33 33 33 33 45 45 45 41 39 39 51 51 41 45
+45 45 45 35 33 33 33 33 33 40 51 51 51 51 51 51 51 51 51 43 45 39 34
+30 30 33 33 33 33 45 45 38 38 38 38 33 30 29 14 15 27 27 30 33 51 51
+41 41 37 34 37 37 37 40 40 40 37 45 51 33 33 33 33 33 33 37 37 31 45
+33 25 22 27 21 28 33 28 28 33 41 37 45 41 33 45 33 22 22 33 33 34 44
+44 45 45 33 33 33 33 33 30 34 34 34 34 37 37 29 34 37 33 33 33 33 33
+33 34 40 33 33 33 30 30 30 37 37 37 30 30 33 30 33 33 49 49 45 45 45
+45 45 45 44 38 38 38 44 38 45 45 49 40 40 35 35 35 35 38 37 40 37 37
+34 34 34 34 34 34 34 36 36 36 34 36 33 31 31 36 33 28 28 27 23 19 17
+17 23 23 23 33 27 28 28 28 30 33 40 40 31 31 17 20 20 28 32 25 17 17
+32 27 37 37 36 33 33 33 33 32 36 34 28 30 32 31 31 14 18 10 9 9 19 21
+30 31 38 38 38 37 37 31 34 34 36 31 30 31 31 31 31 33 33 33 39 30 25
+24 24 29 33 33 34 33 33 23 23 12 23 23 30 33 33 37 37 37 38 40 37 34
+33 37 36 33 33 33 33 33 33 36 28 28 22 24 24 24 21 29 33 33 33 34 33
+33 33 33 33 33 33 33 33 27 28 28 31 31 23 27 19 15 11 20 21 33 33 31
+31 31 31 31 26 28 24 24 24 29 24 29 29 33 27 31 27 30 36 29 18 20 12
+17 9 9 9 19 19 15 8 8 13 15 14 8 9 9 9 10 12 4 4 4 9 10 11 12 24 26 18
+13 13 11 11 13 10 16 14 13 12 4 4 4 17 11 7 7 8 7 8 8 8 20 20 26 16 14
+4 4 4 8 8 10 18 4 4 4 8 8 21 11 13 10 8 10 6 6 6 6 6 8 9 12 14 17 11
+14 14 8 8 8 7 9 7 8 8 12 9 9 8 8 7 10 10 12 10 9 8 16 9 8 8 8 7 6 6 8
+6 6 6 6 6 6 6 9 12 7 7 8 8 7 7 7 12 8 7 7
+
+
+Sequence : 22ak70f10.s1t
+Is_read
+Padded
+Staden_id 2
+Clipping QUAL 35 508
+Align_to_SCF 1 644 1 644
+SCF_File 22ak70f10.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak70f10
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 34 "M13mp1"
+Seq_vec SVEC 584 644 "M13mp1"
+
+DNA : 22ak92c4.s1t
+nGGGCGCTGCCTGCAGGTCGACTCTAnAGGATCCCCATTATACGGGTTGAGTGTTTCTAA
+TCTGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAA
+TGTTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGGGTTGGGAATAT
+TCAACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAATCTGAAACATT
+TCTGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCTGTAGTACGTTCTTTATCAT
+TGTTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAGCAAATTCGATTG
+TATTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAGGATTATTGGAGAGAATAAA
+ACAGTGAAGCTTTGGTGTTATGAGGGAATTTTAGATAGAAAAGTGCAGTTTTTCAGTTCA
+TGCTCTTTCATTTC
+
+BaseQuality : 22ak92c4.s1t
+4 4 6 4 6 8 11 14 18 26 32 34 36 34 23 25 10 23 9 23 23 34 29 23 22 4
+4 4 16 14 25 24 29 33 30 33 33 33 33 40 37 40 45 28 28 25 15 18 32 32
+17 14 28 13 13 38 38 40 40 37 37 37 37 38 45 45 45 38 33 32 33 33 33
+33 38 38 38 45 32 30 45 32 34 34 34 34 40 33 33 32 32 28 28 38 45 45
+49 45 45 45 45 40 37 41 45 45 40 30 30 33 33 33 33 40 40 45 45 45 45
+32 32 28 11 12 27 25 33 33 45 41 33 32 14 14 28 33 37 37 37 37 37 45
+45 37 37 37 37 37 37 37 37 28 33 32 21 14 32 20 24 32 21 21 32 36 41
+41 45 32 32 28 13 13 33 33 45 51 51 41 37 32 32 32 32 32 32 34 34 30
+30 31 32 12 12 27 31 30 35 35 35 33 39 41 28 28 28 28 28 28 41 41 41
+30 32 30 30 30 32 51 51 51 51 45 45 45 45 45 45 45 45 51 45 45 45 45
+33 33 33 33 33 33 51 51 51 51 51 41 41 45 45 45 39 39 39 39 39 39 41
+41 41 41 40 33 33 33 33 33 33 41 45 41 41 41 41 33 33 33 33 33 33 51
+51 45 34 22 22 20 28 30 17 15 15 45 30 33 33 33 33 33 33 33 33 37 37
+32 33 33 25 25 9 10 9 14 14 33 33 34 34 41 40 40 40 40 37 40 37 37 34
+33 33 29 24 22 32 32 45 45 40 27 27 14 32 32 45 38 34 45 19 21 10 21
+21 30 37 37 34 34 37 38 38 37 37 37 37 37 35 34 36 36 40 40 49 34 34
+34 34 37 37 38 37 33 30 30 30 30 30 40 40 34 34 34 27 27 31 31 33 35
+33 30 30 21 21 12 30 28 36 40 36 36 36 33 33 26 26 21 19 20 29 30 36
+34 34 33 31 28 28 32 35 32 35 26 30 25 26 26 25 21 19 12 11 19 21 23
+21 11 13 17 17 24 29 27 29 29 28 31 20 25 18 16 12 14 23 21 11 11 16
+14 10 21 23 33 33 34 31 31 22 22 14 21 11 16 18 31 40 40 36 36 24 27
+19 19 13 16 10 8
+
+Sequence : 22ak92c4.s1t
+Is_read
+Padded
+Staden_id 16
+Clipping QUAL 37 490
+Align_to_SCF 1 494 1 494
+SCF_File 22ak92c4.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak92c4
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 36 "M13mp1"
+
+DNA : 22ap08f1.s1t
+GAACTATCATnnnATATACGGGTTGAGTGTnTCTAATCTGAAAATCCAAAATTAGAAATG
+CTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAATGTTCAAAGGAAGTGCTTATTGGA
+GCATTATGGGTTGTTAgATTTTTGGGTtGGGAATATTCAACCATTAAGCTACACACAATG
+CAAATATCCCACAAACAATCTGAAATCTGAAACACTTCTGGTCCAAGCAAGCATCCGCAA
+CGGATACGCAACCTGCACACGTTCTTATCATCGTTAACTCTnATATATGTGGACAAATCT
+GACGTGGTACACACACTCATGTATATCACAACATATTACATTTGAAGCTGCTACGAATAT
+GGAAGAATnnnnCCTGAACTTGGTGTATGACGAACTTAGATAGACAGTGACTCTTCAGTT
+CAGCTCnTCATTTnTnnTCCCTCGGTTTTnCTAAnCnCnnCnnAAAAnnnnATCCnGTGG
+GCATAAAnnCnGGTCCCACCGCCCCCnCCCCACAAAACCACACACTACCGACCTCCCAA
+
+BaseQuality : 22ap08f1.s1t
+7 9 10 13 14 16 21 15 14 4 4 4 4 4 11 11 20 26 28 28 30 30 30 25 25 20
+25 23 28 4 4 4 18 18 18 21 24 31 33 34 33 34 34 40 30 31 30 24 24 24
+31 27 30 31 30 23 32 32 37 36 38 34 33 24 18 15 10 8 8 18 25 32 33 34
+31 31 33 29 21 12 9 7 8 11 13 18 22 25 25 33 33 32 29 22 27 24 25 16 9
+9 19 21 20 14 16 10 12 12 11 11 18 23 21 19 19 10 10 10 20 9 9 13 18
+18 20 21 18 17 14 16 9 9 9 10 10 10 9 9 21 25 22 18 18 14 20 21 13 13
+21 17 26 25 23 20 15 10 12 16 17 15 10 11 8 8 8 8 6 4 4 4 8 10 8 8 6 6
+8 13 15 21 21 15 11 9 12 11 7 6 6 6 6 6 6 10 6 6 6 6 10 14 10 10 9 12
+9 11 10 14 9 13 10 10 12 9 9 15 17 20 16 17 21 25 10 8 10 10 7 7 10 9
+7 8 14 7 7 8 10 8 6 6 6 8 8 10 11 14 8 9 11 19 17 13 9 9 9 8 8 10 9 8
+8 8 9 8 11 11 10 8 8 8 8 6 6 8 10 8 7 6 4 8 4 4 4 7 7 11 10 12 9 12 10
+10 10 11 6 6 9 9 14 8 7 7 7 7 12 16 11 6 6 6 6 6 6 6 6 6 8 13 9 8 8 15
+12 10 9 8 8 8 8 7 9 12 8 6 6 8 4 6 6 7 11 13 13 7 8 9 7 7 12 12 10 9 4
+4 4 6 7 7 12 14 7 7 12 6 6 6 7 4 4 4 4 4 4 8 8 14 12 13 16 16 16 10 11
+16 14 12 7 6 6 4 4 4 8 7 7 7 12 7 7 8 11 4 4 4 4 4 6 6 6 6 6 6 6 6 6 4
+4 8 6 6 12 12 12 9 8 4 4 4 6 11 6 9 4 4 4 4 4 4 9 9 9 15 9 9 6 6 6 8 4
+4 4 9 9 4 4 4 4 4 4 4 4 4 4 4 9 9 4 4 4 4 4 4 11 12 4 4 4 8 8 15 9 6 8
+9 9 9 4 4 4 4 4 4 7 10 6 8 15 8 8 4 6 14 15 17 18 4 4 4 14 8 6 6 8 8 9
+13 9 9 9 7 7 7 7 8 8 6 6 8 6 6 6 8 6 6 8 8 8 8 4
+
+Sequence : 22ap08f1.s1t
+Is_read
+Padded
+Staden_id 17
+Clipping QUAL 17 155
+Align_to_SCF 1 539 1 539
+SCF_File 22ap08f1.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ap08f1
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Tag DONE 137 137 "AUTO-EDIT: replaced T by g at 137 (terminated, isolated, strong)"
+Tag DONE 148 148 "AUTO-EDIT: replaced A by t at 148 (terminated, isolated, strong)"
+
+DNA : 22ai01c4.s1t
+GGAACATGCTGCAGGTCGACTCTAGAGGATCCCCGCTCTACAGTCTGAAACTTTTTTGAG
+CACCGACCTAATGTTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGG
+GTTGGGAATATTCAACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAA
+TCTGAAACATTTCTGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCTGTAGTACG
+TTCTTTATCATTGTTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAGC
+AAATTCGATTGTATTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAGGATTATTG
+GAGAGAATAAAACAGTGAAGCTTTGGTGTTATGAGGGAATTTTAGATAGAAAAGTGCAGT
+TTTTCAGTTCATGCTCTTTCATTTTTTACTCCCTCAGGTTAAAGCT-GAAGCTCAACAAA
+GATATAGTGATCTCTGTGGGCATTTATAATCTGGTCCAGAAGGCTCTCAAGCCTCCTCCA
+ATAAAGCTCTATCGGGAAACAAATGAAACAGTGAAAACCAAGAACCGGAACTTTAATACA
+AGTACAGGCGGTTTGCTTTCTGCTAGCGATACCAAAAGTCTCAGGTAGGTAAAAATGCTn
+nTGTGTTA
+
+BaseQuality : 22ai01c4.s1t
+4 4 6 8 8 18 18 25 25 30 25 21 27 16 16 27 24 27 31 37 33 33 31 31 21
+21 23 25 37 34 37 45 45 45 45 40 40 40 33 33 30 30 30 30 38 45 45 49
+45 45 45 45 45 45 45 45 38 38 30 30 30 33 33 30 37 37 40 42 42 45 38
+45 35 25 27 33 33 33 33 37 37 37 40 37 37 37 37 42 42 42 40 37 40 45
+33 33 33 33 33 33 45 45 41 41 45 37 30 41 32 41 45 37 37 41 41 45 45
+41 32 38 37 30 30 30 41 41 51 51 51 51 33 33 35 35 35 29 33 33 33 33
+33 45 37 37 37 37 37 40 40 40 40 45 43 35 33 33 33 33 33 43 45 43 32
+33 33 28 28 29 51 51 45 51 51 51 51 51 51 51 51 51 51 51 51 51 51 42
+42 42 40 39 39 51 51 45 45 45 38 38 37 37 38 33 33 33 33 33 33 37 40
+39 39 42 33 33 33 33 33 33 37 37 41 41 41 41 33 33 33 33 33 33 51 45
+41 41 39 34 34 29 31 37 34 34 45 37 38 42 33 32 32 32 32 32 37 51 32
+32 32 32 32 21 24 12 25 25 33 33 33 33 40 40 40 37 37 41 45 45 45 35
+34 34 34 34 34 41 41 45 45 45 41 37 37 41 40 45 37 37 42 33 33 22 33
+33 33 37 37 37 37 37 37 37 37 37 36 33 33 33 33 33 33 40 40 45 34 34
+34 34 34 34 37 37 40 33 33 33 33 33 36 36 40 34 37 37 37 37 38 38 36
+33 33 33 31 31 20 29 27 34 34 34 40 40 34 34 38 38 38 34 34 40 37 40
+37 30 30 30 30 30 34 36 40 40 36 36 28 33 32 27 27 18 14 14 18 23 27
+34 33 29 29 24 29 29 27 27 28 28 25 25 33 22 24 23 31 28 33 34 31 25
+25 25 25 30 31 36 32 27 18 13 13 25 25 17 23 25 31 45 45 45 38 31 31
+24 22 20 27 35 34 31 33 33 33 33 36 36 36 38 20 13 10 8 12 11 18 20 23
+34 34 30 1 29 22 19 24 22 24 28 31 31 31 27 27 23 28 10 25 20 17 10 25
+25 35 35 38 38 36 36 27 27 17 17 11 13 16 19 18 18 18 22 15 11 11 15
+27 25 24 24 18 8 9 8 7 7 8 7 8 8 15 17 21 14 10 10 19 17 20 25 24 24
+21 16 15 21 21 26 33 33 26 23 19 23 23 28 21 20 15 10 8 7 8 6 8 11 7
+10 18 17 12 9 9 7 11 9 7 6 7 6 8 9 9 9 6 10 8 10 7 8 8 7 6 6 8 7 8 8
+14 23 24 19 14 14 7 7 7 7 8 8 6 6 7 6 6 6 6 6 9 21 13 12 13 15 15 10
+11 17 16 15 8 7 7 9 9 10 10 8 8 8 8 9 8 6 8 8 8 7 7 7 7 7 7 7 7 7 7 7
+10 8 7 7 8 11 4 4 4 4 9 6 6 6 6 6
+
+
+Sequence : 22ai01c4.s1t
+Is_read
+Padded
+Staden_id 6
+Clipping QUAL 35 553
+Align_to_SCF 1 466 1 466
+Align_to_SCF 468 668 467 667
+SCF_File 22ai01c4.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ai01c4
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 34 "M13mp18"
+
+DNA : 22ak93c1.s1t
+nGGTGAAGCTTGCATGCCTGCAGGTCGACTCTAAAGGATCCCCACAcCAAAGCTTCACTG
+TTTTATTCTCTCCAATAATCCTAAgCAAGCTCTCAAAATATAAATATGCTTTtTAATAAT
+ACAATCGAATTTGCTATACCACCTCAGAATCTGTACCACAATATATTAACTACTTAAAAC
+AATGATAAAGAACGTACTACAGGTTGCGTATCCCTTTGCAAAATGCTTGCTTAgGACCAG
+AAATGTtTCAGATTTCAGATTTTTTTTGGAATATTTGCATTTTATAGTACTTACTGGTTG
+AATATTCCCAACCCAAAaATCTAACAACCCATAATGCTCCAATAAGCACTTCCTTTGAAC
+ATTATGTCGGTGCTCAAAAAAGTTTCAGACTGTAAAGCATTTCCTAATTTTGGATTTCCA
+GAATAGAAACACTCAACCCGTATAATAAAATGATAATCTGTATTAAGCACAGCACTATAT
+CAAGCACCTCACAGCTATTCTCTCATTTAATCATCATAATAACCCTGTAAGTACTGTACA
+AGTGGTCTGGTCCCTGCTTTTATTTCTCCAAGAACGACTCTCACACTATTGGTTCGCCTG
+GAGTGCnTCGCGTGATCCCCGCTCATGGCACCTCCACTCCCGGGTCCAAGCAATTCTCTT
+GCTCAACTTCCAAGTGGTGGGATTAGGGCCCTGCCATAACCCACTTATTTCTTTTATTTT
+AATAAAACGTTTCCCCATTTTGGCAGTGGGTTTTACCCTGACCCGTATTGCCCTTCCGGC
+CCACGATTTGGGGGAACCAGTTGGGCCCCCCTTTGGGCCTCTCCTGTTCCGAGC
+
+BaseQuality : 22ak93c1.s1t
+4 4 4 4 8 8 12 20 25 31 33 33 30 30 30 31 31 35 35 34 27 23 12 11 25
+11 14 14 28 32 20 14 6 6 6 6 8 16 16 25 28 15 15 13 17 9 10 9 16 16 18
+18 18 25 27 30 26 20 21 19 10 10 14 23 25 30 30 37 37 37 34 31 28 30
+22 18 29 18 22 18 15 11 21 12 15 15 22 24 28 37 37 37 37 32 27 10 10
+13 18 20 25 25 23 23 24 32 33 40 40 38 32 18 18 18 27 27 28 37 37 21
+21 15 15 12 22 32 23 23 30 30 45 45 37 37 32 32 23 22 22 22 27 28 31
+26 23 12 12 18 32 38 37 37 37 37 34 34 30 30 30 30 30 30 34 34 37 37
+37 34 34 31 31 31 28 27 24 22 22 22 30 30 33 30 30 32 26 24 15 15 9 7
+7 14 10 10 9 14 11 12 10 11 6 7 9 8 17 24 26 28 26 28 28 35 34 34 30
+26 26 26 28 28 31 31 33 33 31 23 20 15 13 15 18 14 6 6 8 13 13 19 9 7
+7 16 10 10 15 10 8 8 12 10 9 9 18 13 14 17 11 7 7 13 21 23 28 21 20 19
+19 22 25 27 31 32 32 28 28 25 25 25 25 25 25 31 30 19 19 13 13 13 27
+28 33 33 33 33 33 36 33 31 24 21 14 15 18 24 27 31 25 28 30 30 28 23
+23 21 17 14 8 9 9 25 25 33 25 25 28 30 30 30 22 21 20 20 22 25 28 34
+28 21 16 15 10 14 17 25 25 12 12 12 12 12 20 10 14 16 21 17 13 11 9 12
+11 7 6 6 6 8 14 8 8 6 6 11 23 21 28 28 31 31 34 23 10 7 7 7 7 7 9 8 8
+14 16 20 20 16 10 7 7 7 14 7 9 14 10 9 14 9 19 22 27 25 13 13 18 14 17
+23 16 9 8 9 7 7 7 7 9 8 8 13 16 21 19 15 15 10 10 15 11 18 25 23 21 21
+21 32 25 28 25 28 17 20 10 7 7 11 6 7 6 6 7 9 8 6 6 6 6 6 8 7 7 9 13
+11 13 13 20 21 18 15 10 10 9 9 8 8 8 9 9 13 16 16 14 14 14 25 10 11 6
+6 6 6 7 10 14 9 13 20 8 7 7 8 11 10 13 12 12 13 16 14 15 15 13 13 10
+12 7 8 9 14 11 12 12 9 10 8 8 8 10 20 12 9 9 12 6 6 6 6 8 9 6 6 6 6 7
+6 6 8 16 18 18 9 6 6 6 13 7 7 7 8 9 9 7 7 7 7 7 6 6 6 6 6 6 6 6 7 8 8
+10 9 8 8 6 7 8 4 4 4 4 4 10 7 7 9 9 8 8 4 4 4 4 4 4 8 4 9 9 12 9 8 6 6
+6 9 11 7 7 7 7 7 9 11 11 9 6 6 6 10 8 8 7 10 10 14 25 16 10 7 7 7 7 10
+10 8 8 7 7 7 8 6 6 6 6 8 11 12 7 6 13 6 7 7 7 8 7 7 7 8 8 10 8 6 6 6 4
+4 8 8 10 13 22 24 24 20 14 8 6 6 6 6 6 10 15 15 10 9 6 6 10 8 7 7 9 9
+13 13 10 11 11 11 8 7 7 6 14 6 7 8 6 6 7 7 7 7 7 7 12 7 8 10 10 13 14
+9 8 8 8 11 9 7 7 7 8 8 11 10 12 11 11 10 10 12 12 17 13 9 8 9 6 6 6 6
+6 12 8 8 8 8 9 8 8 9 8 7 7 7 9 9 8 8 6 6 8 8 11 12 8 6 8 6 6 6 6 6 7 8
+9 11 12 12 6 6 8 7 8 8 13 7 7 7 6 8 8 8 8 8 8 6 6 8 4 6 4 4 4 4
+
+Sequence : 22ak93c1.s1t
+Is_read
+Padded
+Staden_id 10
+Clipping QUAL 44 345
+Align_to_SCF 1 834 1 834
+SCF_File 22ak93c1.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak93c1
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 43 "M13mp1"
+Tag DONE 47 47 "AUTO-EDIT: replaced T by c at 47 (terminator, isolated, strong)"
+Tag DONE 85 85 "AUTO-EDIT: replaced C by g at 85 (terminated, isolated, strong)"
+Tag DONE 113 113 "AUTO-EDIT: replaced A by t at 113 (terminated, isolated, strong)"
+Tag DONE 234 234 "AUTO-EDIT: replaced C by g at 234 (terminated, isolated, strong)"
+Tag DONE 247 247 "AUTO-EDIT: replaced C by t at 247 (terminated, isolated, strong)"
+Tag DONE 318 318 "AUTO-EDIT: replaced G by a at 318 (terminated, isolated, strong)"
+
+DNA : 22ak65e10.s1t
+TGAGGGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAAAATAAATAAATAA
+ATAAATTAGCTGGGTGTGATGTCACATGCCTGTGATCCCAGCTACTTGGGAGGCTGAGAC
+AGAAGGATCGCTTGAGTCCAGGAGGTCAAGGCTGTAGCGAGTCATGTTCGCCCCACTGCA
+CTCCAGCCTGGGCGACACAATGAGACCCTGTCTCAAAAACAACAACAACAAAAGGCATCT
+CTACCTACCTGAGACCTCTTGGTATCGCTAGGCAGAAGCAAACCGCCTGTACTTGTATTA
+AAGGTCCGGGTCTTGGTTTTCACTGGTTCATTTGTTTCCCGATAGAGCTTTATTGGAGGA
+gGCTTGAGAGCCTTCTGGACCAGATTATaAATGCCCACAGAGATCACTATATCTTTGTTG
+AGCTTC-AGCTTTAACCTGAGGGAGTAAAAAATGAAAGAGCATGAACTGAAAAACTGCAC
+TTTTCTATCTAAAATTCCCTCATAACACCAAAGCTTCACTGTTTTATTCTCTCCAATAAT
+CCTAAGCAAGCTCTCAAAATATAAATATGCTTTTTAATAATACAATCC
+
+BaseQuality : 22ak65e10.s1t
+4 4 8 8 9 9 14 15 31 31 28 28 28 30 30 33 33 33 30 22 20 12 22 12 18
+18 30 31 27 23 32 10 10 14 12 32 32 37 45 45 45 45 45 38 34 29 29 29
+29 29 29 29 32 32 30 34 34 34 34 35 34 34 32 35 29 27 32 32 32 37 45
+32 45 37 37 37 24 24 32 32 19 22 11 21 21 31 31 34 45 45 45 28 30 30
+37 40 34 33 33 28 28 28 37 45 41 41 41 41 45 41 27 27 33 37 37 32 27
+20 20 20 30 20 20 21 24 17 38 32 30 42 37 37 37 30 28 13 12 18 25 21
+24 14 25 25 22 28 17 28 28 18 32 36 36 32 32 21 32 32 31 31 27 27 21
+32 32 27 13 13 32 33 38 45 36 34 30 30 21 21 23 23 23 23 30 33 33 37
+34 41 36 41 37 37 34 29 29 19 30 30 40 37 37 36 37 25 25 26 26 30 45
+41 51 37 33 30 28 28 33 20 18 20 20 20 20 26 20 20 18 21 21 31 31 28
+32 14 18 20 29 33 34 45 45 40 40 40 37 37 37 37 37 32 45 30 23 23 32
+32 37 34 35 34 34 34 34 37 37 37 37 45 37 37 32 27 23 14 12 12 14 17
+19 27 27 28 30 37 45 45 45 45 37 37 37 42 42 40 37 37 34 33 33 40 31
+26 28 25 27 31 34 34 34 31 27 27 27 31 27 31 23 23 18 18 18 25 25 31
+35 31 30 27 25 27 35 33 32 30 21 21 21 23 23 27 26 21 20 16 16 17 18
+30 30 36 36 36 34 34 36 34 26 15 12 6 6 6 12 11 23 23 26 17 17 21 32
+35 35 40 33 33 33 38 33 27 23 23 12 12 18 18 19 10 8 8 17 19 34 33 34
+31 34 31 31 22 22 19 18 27 27 32 22 25 24 32 32 34 34 34 37 34 30 33
+27 27 27 31 32 27 27 18 1 12 12 11 15 15 17 27 28 28 34 34 30 25 18 13
+19 15 15 25 21 29 26 34 24 28 25 27 21 20 23 24 24 26 29 30 26 27 22
+29 27 33 34 34 36 38 38 38 34 31 30 24 24 24 31 33 35 35 29 33 33 31
+31 28 28 29 26 22 14 8 9 9 9 14 9 13 18 21 21 27 23 18 15 21 21 25 33
+33 31 31 21 21 24 27 29 25 19 19 19 25 27 27 34 34 34 14 9 7 7 9 18 23
+27 23 19 19 23 24 21 18 8 8 9 21 20 31 24 20 13 11 16 24 32 32 25 18
+13 13 20 20 26 21 27 21 20 13 17 15 21 12 12 18 15 15 15 15 8 8 7 10 8
+4
+
+
+Sequence : 22ak65e10.s1t
+Is_read
+Padded
+Staden_id 4
+Clipping QUAL 42 575
+Align_to_SCF 1 426 1 426
+Align_to_SCF 428 588 427 587
+SCF_File 22ak65e10.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak65e10
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 41 "M13mp"
+Tag DONE 361 361 "AUTO-EDIT: replaced C by g at 361 (all_stranded, compound, strong)"
+Tag DONE 389 389 "AUTO-EDIT: replaced C by a at 389 (terminated, isolated, strong)"
+
+DNA : 22ak93c2.s1t
+CTGGGAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCACTCATTTTCTGCCTGG
+TGTTTATTCTCATCCAAATTCGTATACATGCTCGGTGTGAATGGTTACTCATTATAGTAT
+ACAATCGACTTTCTCAAACGACCACACATGCTGAATATCTATACATGTGCTAGTTGGACC
+TCTGATTATCAACGTGAGACTGGTGGCGTATCCCTTTGCAAAATGCTAACAAAAGACCAC
+AAACGTGTCACATTTCAGAATTGCTTGGAATATTCGCGTGTATAGTACTTACTGGTTCAA
+GGGTCCAAAACATACCTCTACACACCATCCTGATTCCCTGGGACACTTCCTTTGAACATC
+ACGTCGGTGCTCAAAAAAGTGTCAGACTGTAGAGCATATCTGGTTTCGGAATGTCGAGAT
+AGAAGGTCTCAAGCCGTATAAATAAACGAGAGTCTGTATCAAGCACTGCAGGAGAATCAT
+AACCTCACAGCTATGCTCTCATGGAAACATCTCAATACACCTCGTAGTACTGTACTAATA
+GTCATGTCGCTGCTTTTTGTTnCCGACAACCAATCTCACGGATTGGTTCGGCCGGTTTGC
+ATCCCTGATTCCGATCATTGAAGGTCGAACTCCCGGTCCAAGCAATCTCCTGCCCCGGCT
+CCAGATGACGGGATTCTTGCCTTTCAACACCCCGGCTAATTCATCCTCTCCCTCGACACC
+GATTTCACGAAATGGGGGTCGGCCCCAACCCGAACATTTGTTTACTGGGTAAAACGGTTC
+CCCAGTTACCCGTTGGCCCACTCCTGGGCCATTAATTGCCTC
+
+BaseQuality : 22ak93c2.s1t
+4 4 8 8 9 12 12 22 27 30 30 30 30 33 33 35 33 37 37 33 31 30 25 25 27
+27 18 18 32 38 32 28 28 9 9 10 10 25 21 27 38 28 25 23 16 12 10 10 11
+10 13 12 11 15 14 8 8 11 13 18 21 21 9 7 7 6 11 8 8 8 6 6 8 8 8 8 10 9
+9 10 9 14 9 13 11 8 8 8 8 8 11 10 11 14 14 14 21 11 10 10 10 11 17 11
+7 7 8 8 9 9 13 14 18 15 9 9 9 8 8 10 12 12 10 10 8 9 12 12 13 14 23 10
+10 10 12 11 11 10 10 10 11 14 14 15 15 21 22 10 10 10 10 28 10 9 9 9
+10 12 15 18 15 15 12 12 12 12 25 21 24 27 18 13 13 13 14 12 14 12 10
+10 10 14 18 14 9 9 11 12 12 10 10 10 10 9 14 14 8 8 8 8 6 6 9 6 6 6 12
+18 34 36 38 38 38 38 36 36 33 33 23 23 13 13 15 23 28 20 9 6 6 4 6 10
+8 7 7 9 10 9 13 13 10 10 9 8 8 6 6 8 8 11 12 10 9 9 9 6 4 6 6 8 6 7 11
+10 9 7 8 17 18 21 19 17 11 8 8 9 11 11 15 12 8 8 8 9 9 15 15 10 10 9
+10 7 7 8 12 10 10 9 9 9 9 10 11 10 8 8 8 7 12 7 7 7 8 9 8 9 10 10 9 10
+8 6 6 6 6 7 6 9 10 10 10 7 6 6 6 8 6 6 9 10 13 12 12 9 12 15 14 14 8 8
+8 9 10 10 9 9 9 9 8 6 6 10 8 13 6 6 6 11 16 18 12 11 8 10 12 8 7 9 7 7
+7 7 9 9 7 7 10 10 12 10 20 9 7 7 7 8 8 8 9 7 7 7 11 9 13 19 15 12 7 8
+7 6 6 6 6 6 6 6 10 13 8 6 6 8 6 8 10 10 12 7 7 7 7 7 7 8 10 9 9 8 7 6
+6 8 6 6 8 9 9 10 7 7 8 8 8 8 7 7 8 7 7 7 7 7 9 8 8 15 8 8 6 6 6 6 6 6
+7 10 9 9 8 7 8 8 10 7 7 8 8 11 11 11 8 6 6 6 6 6 12 6 6 6 6 6 7 11 12
+9 7 10 11 8 8 7 8 11 8 7 7 7 6 8 6 6 8 6 8 7 7 8 7 7 7 8 9 7 7 7 7 7 7
+9 7 7 7 6 13 4 7 7 7 12 23 21 14 12 9 8 9 8 4 4 4 6 6 6 7 6 8 4 4 4 6
+6 6 6 6 6 8 6 7 10 9 9 9 8 6 4 4 4 6 8 6 6 6 6 8 6 6 8 8 8 8 8 8 6 6 8
+11 10 7 7 7 7 9 9 9 8 7 11 8 7 7 8 9 8 8 7 7 7 7 7 7 7 7 8 7 7 7 7 8 7
+7 8 7 7 7 7 8 7 7 7 7 7 7 6 6 7 7 6 6 6 8 9 8 7 7 7 7 8 8 12 9 7 7 7 7
+6 7 8 7 7 7 8 8 6 6 6 6 6 6 7 7 9 12 10 14 8 9 8 6 6 6 6 6 6 9 10 10 9
+6 6 6 6 8 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 7 7 7 6 6 6 4 6 6 6 6 8 6
+6 6 6 6 8 8 10 7 4 7 4 4 4 6 6 6 6 6 6 6 6 6 6 6 7 7 9 9 8 8 7 9 9 7 7
+7 8 6 6 6 6 6 6 6 6 6 6 8 8 8 8 8 8 8 7 7 6 6 6 6 7 8 9 9 9 9 7 7 7 7
+7 7 7 6 6 6 6
+
+Sequence : 22ak93c2.s1t
+Is_read
+Padded
+Staden_id 13
+Clipping QUAL 44 45
+Align_to_SCF 1 822 1 822
+SCF_File 22ak93c2.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak93c2
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 43 "M13mp1"
+
+DNA : 22am84d9.s1
+CAACCCGCACACCTGCACGTCGACTCTAGAGGATCCCCATAAATAAATAAATtAgCTGGG
+TGTGATGTCACATGCCTGTgATCCCAGCTACTTGGGAgGCTGAGACAGAAGGATCGCTTG
+AGTCCAGGAGGTCAAGGCTGTAGCGAGTCATGTTCGCCCCACTGCACTCCAGCCTGGGCG
+ACACAATGAGACCCTGTCTCAAAAACAACAACAACAAAAGGCATCTCTACCTACCTGAGA
+CCTCTTGGTATCGCTAGGCAGAAGCAAACCGCCTGTACTTGTATTAAAGGTCCGGGTCTT
+GGTTTTCACTGGTTCATTTGTTTCCCGATAGAGCTTTATTGGAGGAGGCTTGAGAGCCTT
+CTGGACCAGATTATAAATGCCCACAGAGATCACTATATCTTTGTTGAGCTTCAAGCTTTA
+ACCTGAGGGCAGTAAAAAATGGAAGAGCATGAACTGGCAAAATGCACTTTTCTATCTAAA
+ATTCC
+
+BaseQuality : 22am84d9.s1
+4 9 9 9 9 10 10 9 9 9 9 10 10 10 8 8 6 6 10 8 11 12 15 14 13 15 11 11
+11 12 14 10 10 10 17 23 31 31 21 20 13 21 11 10 10 16 14 14 14 23 11
+11 10 10 10 11 11 12 32 18 23 23 32 32 34 37 34 37 37 38 27 18 18 17
+24 30 32 32 10 10 11 23 23 38 37 37 37 37 37 37 37 37 37 37 32 32 22
+22 22 32 28 40 37 37 37 45 32 32 28 22 22 12 16 16 32 32 37 37 37 37
+28 28 28 32 32 30 30 27 29 29 34 37 37 37 37 37 37 37 37 37 28 22 20
+30 30 29 37 34 37 37 37 37 37 37 37 37 37 37 37 45 45 37 37 37 37 45
+37 37 34 34 34 33 31 33 34 37 27 27 13 13 13 21 32 34 36 31 31 33 19
+17 14 9 9 13 24 27 27 33 33 33 33 33 33 33 33 33 36 36 37 37 37 37 37
+45 45 36 34 34 28 31 31 34 34 37 38 34 38 38 33 30 30 30 30 30 33 34
+37 37 37 34 34 34 34 34 35 35 33 33 33 33 33 33 34 30 30 30 30 30 30
+33 33 30 30 30 34 34 34 34 31 31 31 36 36 34 31 17 13 8 8 15 18 27 31
+28 28 28 28 28 30 31 35 35 34 34 34 34 33 33 33 34 34 38 38 38 37 32
+28 22 19 19 25 27 37 30 30 30 28 28 28 38 37 34 34 37 31 31 25 24 28
+30 30 28 28 28 28 28 28 34 34 34 33 33 31 31 27 24 18 15 18 18 23 23
+28 27 22 18 11 11 16 21 24 33 34 23 23 8 8 9 12 14 17 20 20 20 25 25
+27 27 23 16 11 15 14 9 9 19 17 18 22 18 11 11 11 12 17 18 31 31 31 31
+27 27 33 29 22 18 17 17 20 20 17 13 11 11 11 6 8 14 17 22 22 25 25 20
+20 20 17 14 4 4 4 14 15 21 22 18 11 12 7 7 7 7 7 12 21 18 15 13 9 9 15
+12 8 6 6 6 6 9 6 6 6 12 14 13 13 17 21 21 24 21 22 18 14 18 16 14 14
+10 6 7 6 13 8 8 7
+
+
+Sequence : 22am84d9.s1
+Is_read
+Padded
+Staden_id 7
+Clipping QUAL 39 427
+Align_to_SCF 1 485 1 485
+SCF_File 22am84d9.s1SCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_primer
+Template 22am84d9
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 38 "M13mp18"
+Tag ALUS 41 210
+Tag DONE 53 53 "AUTO-EDIT: replaced C by t at 53 (all_stranded, isolated, strong)"
+Tag DONE 55 55 "AUTO-EDIT: replaced C by g at 55 (all_stranded, isolated, strong)"
+Tag DONE 80 80 "AUTO-EDIT: replaced C by g at 80 (all_stranded, isolated, strong)"
+Tag DONE 98 98 "AUTO-EDIT: replaced C by g at 98 (all_stranded, isolated, strong)"
+Tag AMBG 338 360 "AUTOEDIT: Check this edit cluster!"
+Tag CONF 413 413 "AUTOEDIT: possible compression"
+
+DNA : 22ak66f10.s1lt
+CCCGCGGGGGAGGAGGGAnGGGGGGAGAGGAGnAnAAnnnnnnnnnnnnnAGCCCCAAnn
+nAnnCCCTCTTnnnnnnnnAGACGGGGGGnGCCCTGGGnGnGCnTnnnnnnnnnnTTGGT
+TGAGGTGnCGAGCAAATCTTGCATGCCTGtcCGTCTACTCtAGAGGATCCCACAAATGAA
+CCAGTGAAAACCAAGACCCGGACCTTTAATACAAGTACAGGCGGTTTGCTTCTGCCTAGC
+GATACCAAGAGGTCTCAGGTAGGTAGAGATGCCTTTTGTTGTTGTTGTTTTTGAGACAGG
+GTCTCATTGTGTCGCCCAGGCTGGAGTGCAGTGGGGCGAACATGACTCGCTACAGCCTTG
+ACCTCCTGGACTCAAGCGATCCTTCTGTCTCAGCCTCCCAAGTAGCTGGGATCACAGGCA
+TGTGACATCACACCCAGCTAATTTATTTATTTATTTATTTTTTAAGAGACTGGATCGACT
+GGGCACAGTGGCTCATGCCTGTAATCCCAnCACTTTGGGAGGCCGAGGCAGGTGGATTAC
+CGAGGTCAGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCCCATCTCTACTAAAAA
+TACAAAATGAGCTGGGCATGGTGGTGCATGCCTGTAATCCCAGCTACTCCGGAGGCTGAG
+GCAnGAGAATCCCTTGAACCCGGGAGGCAAAAGTTGCGGTGAGCCGAAATCTCGCCATTG
+CGCTCCAGCCTGGGCAACAAAAGCGTAACTCCGTCTCCnnnnnnnnAAAAAAAGAGACGG
+TGTTTCGCTATGTTGCCTGGGCTGGGTGTCAAACTCCTGGGTCTTAAAGTAATCCCCCCC
+TGCTTTGGGTTCCCCCAAATTGCTGGGGGTGGCTGGGATTCCCGAACTTTGGGGCCACTG
+TTGCCCCAGCCGTTTTTTTGGTTTCGTTTTGGTTTTGGTTTTGTTTTAATAAAAAAATAA
+AGGGTCTCACCTATGTT
+
+BaseQuality : 22ak66f10.s1lt
+6 6 4 6 8 8 11 8 8 6 6 11 4 4 4 12 6 4 4 4 8 7 6 6 6 6 6 6 6 8 4 4 4 4
+4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 7 7 6 6 6 8 4 4 4 4 4 4 4 4 6 4 6 6
+8 4 4 4 4 4 4 4 4 4 4 4 4 6 6 6 6 6 6 4 4 4 6 6 4 4 8 12 4 4 4 4 4 4 4
+4 4 4 4 4 4 4 4 4 4 4 4 6 6 4 4 4 4 4 4 8 6 4 4 4 4 4 4 15 17 8 4 4 6
+9 21 21 30 28 30 33 33 21 18 8 8 8 10 15 23 21 26 26 19 14 7 7 8 8 10
+18 21 18 10 9 9 18 14 24 24 29 32 34 37 34 36 32 32 30 30 30 34 38 44
+45 37 37 34 27 27 15 15 23 32 40 40 37 37 34 45 45 45 45 45 42 25 25
+25 26 26 20 23 28 30 33 33 33 34 34 34 34 37 45 45 45 45 49 49 49 49
+49 45 45 43 38 38 37 37 40 37 37 34 27 27 12 10 18 14 32 27 27 27 29
+29 12 18 10 15 10 21 10 14 10 19 19 41 45 45 45 45 45 40 40 34 37 37
+45 45 45 45 45 45 45 45 45 40 51 37 37 25 22 18 27 22 25 25 29 29 28
+33 33 40 37 37 37 37 37 37 34 37 37 42 35 32 30 30 37 38 45 45 30 30
+30 33 31 33 32 37 45 45 45 32 30 30 37 33 37 37 40 37 37 37 37 37 37
+37 37 37 37 34 34 34 37 45 45 45 45 45 45 45 45 49 45 45 45 45 33 33
+29 29 33 33 33 33 33 37 40 45 45 45 45 45 45 45 35 35 35 35 35 40 38
+45 45 45 37 37 32 22 14 14 14 32 45 45 45 38 37 31 28 27 30 28 27 22
+27 27 31 31 34 31 28 28 27 31 33 33 33 33 33 33 32 28 28 30 34 37 37
+35 35 35 37 37 33 33 33 33 33 33 31 31 30 30 33 31 31 36 33 33 33 34
+25 25 10 10 10 19 19 34 34 34 34 31 31 34 34 36 38 30 30 26 23 30 31
+34 32 45 37 37 37 37 33 33 40 40 40 36 33 31 31 31 33 33 23 22 4 4 4
+18 23 32 34 36 36 32 28 21 21 21 29 20 28 25 24 18 25 21 27 27 32 32
+34 31 31 34 37 37 37 37 32 32 10 21 10 25 28 32 29 34 28 31 27 27 28
+22 22 27 29 32 32 37 37 37 37 37 37 37 37 35 34 34 34 31 27 27 19 19
+30 34 37 38 34 34 34 34 34 40 38 38 38 38 37 37 37 35 35 32 28 28 28
+30 30 30 33 30 30 22 22 22 32 32 35 35 34 34 35 35 34 37 37 38 30 24
+24 24 24 24 31 38 38 32 27 27 27 35 25 18 12 9 9 11 21 23 18 12 6 6 8
+8 8 9 10 17 10 14 8 9 8 7 4 4 4 16 14 14 12 15 8 8 11 15 14 25 25 25
+28 33 33 33 21 29 7 7 7 8 8 7 8 9 9 8 11 24 22 31 25 23 23 15 13 10 7
+9 9 8 8 12 7 7 7 16 11 18 14 25 25 22 19 16 17 14 8 9 10 13 15 27 28
+31 23 22 14 14 13 15 15 23 21 8 8 6 6 6 6 9 18 20 28 22 22 20 17 14 9
+4 4 4 4 4 4 4 4 4 4 16 10 13 20 23 23 12 9 9 9 17 10 9 7 8 6 6 8 4 4 7
+12 13 14 7 8 8 8 8 8 18 21 16 9 10 9 7 9 7 9 8 10 7 7 7 12 9 9 7 7 8 8
+6 6 8 8 8 6 8 6 6 6 6 7 6 11 6 8 6 6 6 6 6 8 9 9 8 9 12 8 8 8 7 8 8 7
+8 6 6 6 9 8 6 6 6 10 13 11 7 9 7 7 7 7 7 8 9 7 7 9 9 7 4 4 4 7 11 7 7
+9 8 7 6 6 6 6 6 8 8 10 9 8 8 6 6 6 6 7 6 6 4 4 4 4 8 6 8 13 16 20 8 8
+7 7 7 7 7 7 7 8 12 8 8 6 6 6 6 6 6 6 6 6 6 9 8 8 6 6 6 8 6 9 14 9 9 8
+9 7 6 6 8 6 6 8 7 6 6 8 6 6 7 7 7 7 7 7 8 8 4 4
+
+
+Sequence : 22ak66f10.s1lt
+Is_read
+Padded
+Staden_id 9
+Clipping QUAL 140 640
+Align_to_SCF 1 977 1 977
+SCF_File 22ak66f10.s1ltSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_primer
+Template 22ak66f10
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 3 "M13mp18"
+Tag DONE 150 150 "AUTO-EDIT: replaced C by t at 150 (all_stranded, compound, strong)"
+Tag DONE 151 151 "AUTO-EDIT: replaced G by c at 151 (all_stranded, compound, strong)"
+Tag DONE 161 161 "AUTO-EDIT: replaced A by t at 161 (all_stranded, compound, strong)"
+Tag ALUS 283 462
+Tag ALUS 477 767
+
+DNA : 22ak70h4.r1t
+TGCCGCCTTAGATTACAGCTCTACACGGTACCTCTATCGGGAAACAAATGAACCAgTGAA
+AACCAAGACCCGGACCTTTAATACAAGTACAGGCGGTTTGCTTCTGCCTAGCGATACCAA
+GAGGTCTCAGGTAGGTAGAGATGCCTTTTGTTGTTGTTGTTTTTGAGACAGGGTCTCATT
+GTGTCGCCCAgGCTGGAGTGCAGTGGGGCGAACATGACTCGCTACAGCCTTGACCTCCTG
+GACTCAAGCGATCCTTCTGTCTCAGCCTCCCAAGTAGCTGGGATCACAGGCATGTGACAT
+CACACCCAGCTAATTTATTTATTTATTTATTTTTTAAGAGACTGGATCGACTGGGCACAG
+TGGCTCATGCCTGTAATCCCACCACTTTGGGAGGCCGAGGCATGTGGATTACCGAGGTTC
+GGAGTTCAAGACCAGCCTGACCCTTCTGGAGAAACCCCATCTCCACTTTAAATACCAAAT
+GAAGCTGGGCCTGGTGGTGCCTGCCTGTAATCCCCCCTTCTCAGGATGCTGAAGCAGGAC
+ATTCCTTGAACCCCGGAAGCACATGTTGCCGTGACCCAATATCCCCCCTTGTCCCTCACC
+CCGGGCCACCAAACCTTATTCCCTCCCAAATAAAAAAAAAAAAAAACGGGTTCCCTTTTT
+TGCCGGGGGGGGTTTCTACCCC
+
+BaseQuality : 22ak70h4.r1t
+4 4 4 4 4 8 6 6 8 8 12 6 7 11 13 12 6 6 6 6 6 6 9 10 10 10 21 21 26 31
+27 27 13 14 11 11 11 12 19 20 27 18 18 23 23 16 10 10 14 28 18 18 11
+11 9 9 9 10 10 10 21 21 27 23 18 10 9 9 14 18 32 31 37 34 28 28 22 19
+19 27 24 21 18 17 9 9 12 19 24 28 30 30 30 33 33 30 30 37 40 40 45 37
+41 41 44 44 45 32 42 37 32 32 32 32 37 31 31 33 21 21 11 10 22 18 32
+24 27 27 27 27 11 14 10 23 12 23 9 10 10 18 11 25 25 34 45 37 37 34 34
+34 34 34 34 34 33 33 33 34 34 34 34 33 34 28 31 25 21 23 21 21 13 13
+15 15 17 23 21 26 26 31 28 32 23 23 12 22 25 21 19 9 8 8 17 21 23 23
+20 18 17 27 23 21 27 30 38 38 28 28 17 17 13 18 19 27 25 25 28 28 28
+28 25 31 26 26 18 15 13 9 9 15 19 23 25 31 36 36 36 36 36 36 36 36 24
+19 12 13 18 24 31 31 29 29 31 29 29 33 36 40 44 36 33 34 34 37 36 36
+45 45 34 30 28 28 28 22 32 24 22 22 32 32 36 37 27 27 14 25 25 29 23
+22 19 17 19 19 24 25 27 24 18 18 16 16 16 24 22 30 30 28 28 30 27 27
+23 20 22 25 25 25 21 22 21 16 14 19 17 17 19 20 17 11 11 11 11 12 24
+24 22 20 34 17 17 23 23 31 34 27 27 17 13 15 27 27 34 31 31 24 22 19
+16 16 15 32 23 27 18 9 9 9 13 9 14 20 22 21 21 19 17 14 8 6 6 8 8 9 11
+14 8 7 11 15 14 9 9 9 12 8 10 6 8 8 6 4 4 4 14 15 23 21 13 13 11 13 11
+11 6 8 8 6 6 6 7 6 6 6 6 7 6 6 11 15 17 27 20 17 17 13 21 13 14 4 4 8
+6 6 6 6 8 6 8 11 7 8 8 10 10 19 24 16 16 9 9 12 7 7 8 8 8 8 6 6 11 15
+11 10 9 9 11 14 15 11 8 7 7 7 7 12 9 10 10 8 8 14 10 10 18 21 21 18 16
+10 9 11 9 9 14 10 10 8 8 9 8 8 12 14 10 10 11 11 10 9 8 7 7 7 7 4 4 4
+12 10 14 10 8 14 6 6 6 7 6 6 6 6 6 10 8 9 7 7 7 9 14 15 10 10 10 10 9
+8 13 8 4 4 4 4 4 6 6 12 6 6 10 8 8 8 6 9 12 7 6 6 6 8 8 9 7 7 7 8 9 8
+8 6 6 6 6 6 4 6 6 6 6 7 8 8 6 6 8 8 8 8 9 9 8 10 11 9 8 8 8 8 6 6 11 9
+9 10 9 12 8 6 6 6 10 12 10 10 8 8 8 7 10 13 8 13 19 18 13 6 6 6 6 8 6
+7 12 7 7 10 10 7 7 7 7 7 7 9 13 6 10 7 7 12 11 7 6 6 6 6 8 8 8 4
+
+
+Sequence : 22ak70h4.r1t
+Is_read
+Padded
+Staden_id 15
+Clipping QUAL 28 377
+Align_to_SCF 1 682 1 682
+SCF_File 22ak70h4.r1tSCF
+Primer Universal_primer
+Strand Reverse
+Dye Dye_terminator
+Template 22ak70h4
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 27 "M13mp18"
+Tag DONE 56 56 "AUTO-EDIT: replaced C by g at 56 (all_stranded, isolated, strong)"
+Tag ALUS 155 334
+Tag DONE 191 191 "AUTO-EDIT: replaced T by g at 191 (all_stranded, isolated, strong)"
+Tag ALUS 349 579
+
+Sequence : Contig1
+Is_contig
+Padded
+Assembled_from 22ak93c2.r1t 1 293 39 331
+Assembled_from 22ak93c1.r1ta 1 305 31 335
+Assembled_from 22am91h5.r1t 110 439 32 361
+Assembled_from 22aq40a8.s1 221 570 39 388
+Assembled_from 22an02b10.s1t 252 666 32 446
+Assembled_from 22ak67d6.r1t 260 522 30 292
+Assembled_from 22ak64b1.s1t 331 707 40 416
+Assembled_from 22ak81h10.s1t 331 713 36 418
+Assembled_from 22ak66c6.s1t 337 848 37 548
+Assembled_from 22ak93c1.s1lt 1024 482 56 598
+Assembled_from 22ak64e2.s1t 866 487 41 420
+Assembled_from 22ak70f10.s1t 554 1027 35 508
+Assembled_from 22ak92c4.s1t 635 1088 37 490
+Assembled_from 22ap08f1.s1t 639 777 17 155
+Assembled_from 22ai01c4.s1t 682 1200 35 553
+Assembled_from 22ak93c1.s1t 1036 735 44 345
+Assembled_from 22ak65e10.s1t 1499 966 42 575
+Assembled_from 22ak93c2.s1t 1036 1035 44 45
+Assembled_from 22am84d9.s1 1488 1100 39 427
+Assembled_from 22ak66f10.s1lt 1174 1674 140 640
+Assembled_from 22ak70h4.r1t 1190 1539 28 377
+
+DNA : Contig1
+TTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTATAnACTGTGCGTGCGC
+CACCATGCCTGGCTAATTTTTGTATTTTTAGTAGGGATGGGGTTTCACCATGTTGGCCAA
+GCTGGTCTCGAGCTCCTGACCTAGGATTACAGGCCTAAGCCACCGCACCCGGCATGATGG
+GTCTTTATTCTTCAAAGCAGGAGGAAGGGATCCTAGAAAAACAGAGACAAGGCCAAACAT
+GGTAGCTCACACCTGTAATnnnnnCACTTTGGGAGGCCAGTGCGGGTGAATCACGAnGTC
+AGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAnCACCGTCTCTACTAAAATAAAAAGA
+AATTAGCTGGGTGTCGTGGCAGGTGCnTGTAATCCCAGCCACTTGGGAAGCTGAGGCAGG
+AGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCGACTGCACTC
+CAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGACAAGACnACT
+AGTACAGTACTTACAGGGTTATTATGATGATTAAATGAGAGAATAGCTGTGAGGTGATTG
+ATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATACGGGTTGAGTGTnTCTAATC
+TGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAATG
+TTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGGGTTGGGAATATTC
+AACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAATCTGAAACATTTC
+TGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCTGTAGTACGTTCTTTATCATTG
+TTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAGCAAATTCGATTGTA
+TTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAGGATTATTGGAGAGAATAAAAC
+AGTGAAGCTTTGGTGTTATGAGGGAATTTTAGATAGAAAAGTGCAGTTTTTCAGTTCATG
+CTCTTTCATTTTTTACTCCCTCAGGTTAAAGCTnGAAGCTCAACAAAGATATAGTGATCT
+CTGTGGGCATTTATAATCTGGTCCAGAAGGCTCTnnAnnCnnnTCCnnnnnnnCTnnAnn
+nnnnnACAAATGAACCAGTGAAAACCAAGACCCGGACCTTTAATACAAGTACAGGCGGTT
+TGCTTCTGCCTAGCGATACCAAGAGGTCTCAGGTAGGTAGAGATGCCTTTTGTTGTTGTT
+GTTTTTGAGACAGGGTCTCATTGTGTCGCCCAGGCTGGAGTGCAGTGGGGCGAACATGAC
+TCGCTACAGCCTTGACCTCCTGGACTCAAGCGATCCTTCTGTCTCAGCCTCCCAAGTAGC
+TGGGATCACAGGCATGTGACATCACACCCAGCTAATTTATTTATTTATTTATTTTTTAAG
+AGACTGGATCGACTGGGCACAGTGGCTCATGCCTGTAATCCCAnCACTTTGGGAGGCCGA
+GGCAGGTGGATTACCGAGGTCAGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCCC
+ATCTCTACTAAAAATACAAAATGAGCTGGGCATGGTGGTGCATGCCTGTAATCC
+
+
+
+DNA : 22ak93c2.r1t
+GTCGCnCATAAGATTACGAGATCTCGAGCTCGGTACCCTTCAAGCGATTCTCCTGCCTCA
+GCCTCCCGAGTAGCTGGGATTATAGACTGTGCGTGCGCCACCATGCCTGGCTAATTTTTG
+TATTTTTAGTAGGGATGGGGTTTCACCATGTTGGCCAAGCTGGTCTCGAGCTCCTGACCT
+AGGATTACAGGCCTAAGCCACCGCACCCGGCATGATGGGTCTTTATTCTTCAAAGCAGGA
+GGAAGGGATCCTAgAAAAACAGAGACAAGGCCAAACATGGTAgCTCACACCTGTAATCCC
+AnCACTTTGGGAGGCCagTGCGGGTGAATCACGAAGTCAGGAGTTCAAGACCACCCTGGC
+CAACATGGTGTAACACCTGCTCTCCTAAAATTAAACAAAATTTCATGGTTTGCGTGGGCC
+GTCTTGTCTCATCACTTCACTCCTGAGGGCCGGCGCCGGAAAGATATCTTGATCTGCGGC
+GCTCCGACCGTTTTCTTTAAACCTTACAACTCCCGACCTCCTCGCCTATCCTCCCTAAAT
+CCTCGCCAGGCTCGCCTGCTTCAGCCACTCTTTCCTTCGCACCCTCCCCTCTCTTCAATA
+TACTTCACCCGCCCATCCTTCACGCCGGCACGTATCCAATCTCTTCTTATCTTTCCGTAT
+CCAAnTCCCTTCTCCCTCTGCCGCGACCTTCGCCATCCCTCTGCGCGTCCTCTTCC
+
+BaseQuality : 22ak93c2.r1t
+4 4 8 4 4 4 4 4 4 4 4 4 6 8 17 21 14 7 6 6 6 7 7 6 8 14 16 21 15 20 20
+24 26 21 18 18 14 14 19 23 10 8 8 15 20 16 29 26 34 29 39 29 31 29 31
+34 32 27 27 25 19 19 24 31 33 36 34 34 34 26 27 22 32 32 36 28 28 15
+15 15 28 28 34 30 12 12 22 27 31 31 31 31 31 23 24 27 21 24 24 29 27
+27 27 34 34 36 38 38 38 36 36 40 36 37 38 45 45 36 34 33 31 31 34 34
+33 33 28 28 27 23 24 11 11 10 10 18 25 21 20 17 17 17 20 15 24 18 24
+26 23 23 18 20 25 23 30 30 30 33 33 37 37 32 37 37 32 45 35 37 37 37
+40 36 49 49 36 36 34 33 20 15 9 9 8 7 12 22 21 28 28 30 33 36 36 36 34
+31 31 25 31 28 26 26 24 20 17 9 11 8 9 10 23 23 31 23 23 15 9 9 15 33
+26 33 33 31 25 25 22 31 24 23 12 10 12 11 9 8 9 7 7 8 8 9 18 12 9 9 18
+20 26 31 21 21 9 8 8 11 13 21 21 23 15 15 15 15 15 17 17 9 7 9 19 20
+21 21 25 25 25 25 25 23 23 9 9 9 21 16 24 24 24 24 26 33 33 33 31 31
+27 15 17 7 4 4 4 16 20 27 33 34 34 23 15 14 8 9 6 6 9 1 14 16 8 11 15
+23 25 34 36 31 33 16 16 6 6 6 9 8 14 9 11 11 9 13 13 10 8 10 9 9 7 8
+20 20 20 14 14 10 10 10 16 8 8 6 6 8 9 10 7 8 8 8 8 6 8 6 6 8 12 9 8 7
+13 10 8 8 9 8 8 8 9 6 6 6 6 8 6 6 6 6 6 10 11 10 12 12 10 7 6 6 7 6 6
+7 8 7 6 6 7 6 8 6 6 8 8 8 8 6 6 6 8 6 6 8 6 6 11 15 9 9 9 9 9 9 9 9 9
+9 8 9 9 6 6 6 7 9 6 6 6 7 7 7 8 9 7 7 9 9 11 8 11 11 11 9 10 9 8 8 8
+10 6 6 9 8 6 6 6 6 8 8 9 11 9 8 8 8 8 8 8 8 9 9 9 10 10 8 6 6 8 6 6 10
+11 10 6 6 8 6 6 6 6 8 11 8 9 8 8 8 8 8 9 6 6 9 8 8 8 6 6 13 15 12 12 8
+8 6 6 8 9 8 8 9 8 8 6 6 6 8 8 6 6 6 8 8 8 6 6 8 6 6 8 6 6 9 8 8 6 6 8
+8 6 6 8 6 7 8 9 10 11 11 10 9 9 8 10 9 10 10 8 9 13 8 8 8 13 13 11 7 7
+7 11 8 8 10 11 10 9 9 14 7 7 10 10 10 8 8 8 8 6 7 8 8 6 6 6 6 6 6 6 6
+6 8 9 10 8 8 6 6 8 8 8 8 8 8 8 8 8 8 6 6 8 8 9 10 10 4 4 4 6 7 6 6 6 6
+10 8 8 9 15 11 6 6 8 8 8 8 8 8 9 9 8 9 7 7 8 6 6 8 13 10 8 8 7 6 8 6 6
+8 6 7 8 8 8 8 8 8 8 4
+
+
+Sequence : 22ak93c2.r1t
+Is_read
+Padded
+Staden_id 11
+Clipping QUAL 39 331
+Align_to_SCF 1 43 1 43
+Align_to_SCF 44 317 45 318
+Align_to_SCF 319 716 319 716
+SCF_File 22ak93c2.r1tSCF
+Primer Universal_primer
+Strand Reverse
+Dye Dye_terminator
+Template 22ak93c2
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 38 "M13mp18"
+Tag ALUS 43 180
+Tag DONE 43 43 "AUTO-EDIT: deleted C at 43  (terminator, isolated, strong)"
+Tag DONE 254 254 "AUTO-EDIT: replaced T by g at 254 (terminator, isolated, strong)"
+Tag ALUS 269 402
+Tag DONE 283 283 "AUTO-EDIT: replaced T by g at 283 (terminated, isolated, strong)"
+Tag AMBG 298 302 "AUTOEDIT: Check this edit cluster!"
+Tag DONE 317 317 "AUTO-EDIT: replaced C by a at 317 (terminated, compound, strong)"
+Tag DONE 318 318 "AUTO-EDIT: inserted g at 318  (terminated, compound, strong)"
+
+DNA : 22ak93c1.r1ta
+GGGGGGATGATACAATTCGAGCTCGGTACCTTCAAGCGATTCTCCTGCCTCAGCCTCCCG
+AGTAGCTGGGATTATAAACTGTGCGTGCGCCACCATGCCTGGCTAATTTTTGTATTTTTA
+GTAGGGATGGGGTTTCACCATGTTGGCCAaGCTGGTCTCGAGCTCCTGACCTAGGATtAC
+AGGCCTAaGCCACCGCACCCGGCATGATGGGTCTTTATTCTTCAAAGCAgGAGGAAGGGA
+TCCTAGAAAAACAGAGACAAgGCCAAACATGGTAGCTCACACCTGTAATCCCAGCACTTT
+GGGAgGCCAgTGCGGGTGAATCACgAAGTCAGGAGTTCAAGACCATCCTGGCCAACATGG
+TGTAACACCGTCTCTCCTAGGATAAACCGAAATCACCTGGGTGTCCTGGCAAGTGCCTGT
+CATCCCTGACCACTTGGAAAGCTGAGCCGAATCCTTATCCCnGAAAGTAAGGTTACGTGA
+ACCCACATCAAACCACTGCGCTCCAGCCCAAAACATCGTGTTATACTCTGTCGCGACATT
+GATGCCCAAAAAACAGGACCGCTATTGGGAGACACTCCCGGGTAATTGGGATTGAGGAGG
+TTAAAGAAAACTCTTGATCTGAGAAAAAAGACCCTATCCTGGCGAGAAAACCACATGTGG
+ACCACACCGCTTCACTTTGTCTAAGCAAAAAACGCCCCACTTGAAGATGACCCCCCCAGC
+TGAAATCTTCCATTAATCCCCGAACATAAAGGTTTAACCCAAAATTTCCTGTTAGACCGC
+CTTCTGAAGTTTCTAAAATTCTATGGGTAGGGACAAAAGTCCCTCGTTGAATTTCTAGGA
+TACTTCCAAATATCCCCCACCTCTCCCCTGGGATCCCGATAGCTTTCTTGTCCCTAAAAA
+AAACTTTGTTCAAACGGAGAAACCACACCCGGGGAAAATTCCCCTTTTCTTGGGGTTTAG
+GTTTCTTTAAATAAATGCTGGGCACCAATCCTCAAATGAGTTCTTACCCAATCCCATATG
+TTTTTAATCCTCAACAACAACCTTATTTCCTTAAGAGACCTCTCCTCGGAAATCATTTCA
+AAACAAAAAAAACACTTAAGCCTTGGGTCTTTGGGACCCCCCAAAAAAAAAACATAAAGG
+GGTGGCACCGTATACGCCTTGCCCATCTTTCTAGTGGAAGATAACGAAACGGTAGCTGGG
+GCCAACCAGGCATCAGTGGGGGATACCGCTAAATGTTGGGGAACACCCAAAAACCCATCT
+GCAGGCACCGCGAATGCGGAAAAGCGCACGCTATCACAAATGGTATATGGGCACC
+
+BaseQuality : 22ak93c1.r1ta
+8 10 8 9 16 9 9 9 11 8 8 9 10 11 11 10 19 24 26 24 26 22 21 17 13 16
+11 11 10 10 14 14 19 22 24 26 32 32 33 29 29 29 27 27 27 27 27 32 32
+25 27 20 25 19 32 27 29 32 32 32 34 34 21 23 12 14 14 27 32 32 29 28
+27 34 31 34 34 34 37 34 32 32 30 30 28 28 28 30 30 33 33 33 30 30 34
+34 34 37 40 38 37 34 32 34 32 37 32 37 37 28 28 28 33 33 33 33 32 30
+30 29 28 21 23 14 12 30 32 45 45 41 41 30 25 22 33 33 34 23 21 18 12
+12 11 13 27 27 23 23 11 11 9 10 11 18 18 30 15 20 12 11 10 20 12 14 14
+32 32 33 28 28 18 23 18 12 12 20 12 16 13 18 14 15 12 32 23 21 10 10 9
+9 10 16 18 21 9 9 9 18 21 14 14 12 10 14 14 23 27 24 19 19 24 28 30 29
+29 34 34 29 25 17 12 12 16 14 14 23 10 10 10 11 15 21 24 18 25 11 11
+15 32 28 34 34 29 32 32 37 37 34 34 31 28 28 25 22 17 14 11 10 10 8 8
+9 14 8 8 12 17 18 15 13 18 17 17 10 10 10 22 16 24 30 24 28 27 32 19
+12 12 12 30 21 17 9 9 9 9 17 16 24 24 23 20 12 12 7 7 7 14 13 10 10 10
+23 23 22 15 17 18 24 24 15 17 11 10 8 8 8 13 20 25 17 17 17 20 20 18
+10 11 12 12 11 25 10 12 12 9 8 8 8 8 8 12 14 9 9 9 9 10 10 8 8 11 9 9
+9 14 20 20 19 15 15 15 15 11 11 6 6 9 8 10 12 12 15 15 10 9 10 11 7 7
+9 11 14 11 11 10 11 9 14 14 23 19 18 14 12 10 10 10 10 10 16 11 11 9 8
+8 9 8 11 11 9 8 8 10 9 12 13 9 9 8 8 8 8 8 6 6 11 6 6 8 11 9 9 9 6 6
+12 9 9 6 6 8 11 9 8 6 7 9 9 7 8 4 4 4 6 8 6 6 8 8 6 6 8 6 8 12 12 14
+10 9 10 11 9 8 7 7 7 7 10 8 8 6 6 8 6 6 8 10 8 6 6 10 8 10 9 10 10 9 8
+8 6 6 6 6 8 8 8 9 9 9 9 7 7 9 9 10 10 10 13 12 9 9 9 9 7 8 8 8 9 8 8 9
+9 9 8 6 6 8 6 6 6 7 7 9 9 10 8 8 7 7 7 9 10 10 8 6 6 6 6 6 10 10 8 6 6
+8 8 8 7 9 7 10 7 11 8 8 8 8 9 9 9 8 8 6 6 10 11 16 8 7 7 7 6 8 6 6 8
+10 11 9 9 9 7 7 8 7 7 7 7 7 8 11 9 16 16 24 22 15 9 8 13 13 12 12 9 9
+9 9 9 7 7 7 7 7 7 7 8 7 7 7 6 9 11 9 8 8 9 9 9 7 9 10 14 12 9 9 8 8 6
+6 6 6 8 6 6 8 6 6 6 6 6 9 8 10 10 9 10 10 13 10 11 6 7 11 6 6 8 6 6 6
+6 6 6 9 9 7 7 8 8 8 8 8 9 8 9 13 11 8 6 6 6 9 9 8 8 8 8 8 8 8 11 8 7
+10 12 7 7 9 8 6 6 8 8 7 7 10 8 8 7 7 7 8 8 8 8 7 7 7 7 7 10 9 10 12 10
+7 7 6 9 6 6 6 6 7 9 7 7 4 10 9 7 6 8 6 6 8 6 6 9 6 7 7 10 10 10 10 13
+13 8 9 8 8 8 6 7 10 11 10 9 8 6 6 6 6 6 8 8 7 9 7 7 7 7 7 7 7 10 10 10
+8 8 8 7 7 8 8 8 8 6 6 7 7 7 9 8 6 8 6 6 6 8 8 8 7 9 9 12 15 9 8 7 9 8
+8 9 8 8 6 8 12 11 7 7 7 7 7 7 9 11 11 9 9 9 10 9 9 9 8 8 8 8 8 7 7 12
+13 13 15 11 11 7 7 7 9 11 10 12 6 6 6 6 8 7 7 7 8 10 10 10 10 13 9 12
+11 11 6 6 6 6 8 8 6 7 7 12 10 13 9 8 7 7 10 7 7 15 17 15 12 17 20 15
+13 15 6 6 6 6 8 8 6 6 6 7 7 7 10 7 7 10 11 8 8 5 5 8 8 12 12 8 10 6 6
+6 6 8 8 7 7 7 12 10 7 7 9 7 7 9 9 8 9 10 10 8 8 9 11 9 8 9 9 11 11 9 9
+8 6 8 6 6 6 6 6 6 6 6 8 8 8 8 12 6 6 6 6 8 6 7 7 6 8 6 6 8 8 10 11 10
+12 7 8 8 9 12 6 6 6 6 6 6 6 6 8 9 9 9 9 9 8 7 8 7 7 7 8 7 7 7 7 9 9 9
+9 6 6 6 6 14 8 8 8 8 8 6 6 7 7 7 11 9 7 6 6 6 6 10 6 6 6 8 8 7 8 7 7 6
+7 7 8 8 12 13 15 15 21 9 10 9 8 8 10 6 6 6 6 4 4 4 8 8 8 7 6 6 6 6 6 6
+6 6 6 6 6 9 9 9 6 6 6 10 6 10 15 20 12 9 8 6 6 6 6 6 8 8 6 6 6 6 8 12
+13 12 10 9 8 11 8 10 9 7 7 4 4 6 6 8 4 4 4 6 8 6 6 6 8 8 13 10 8 9 9 9
+7 7 9 10 8 8 6 6 6 6 6 6 10 11 10 10 10 7 7 8 6 6 8 6 6 8 10 13 8 11 8
+11 10 6 6 6 7 11 14 11 11 6 6 8 8 8 9 9 9 9 7 7 10 10 6 6 6 6 6 6 8 8
+8 7 7 7 8 8 11 11 11 7 6 8 6 6 8 8 6 6 10 10 13 8 8 6 6 6 8 9 9 6 6 9
+9 8 8 6 6 8 6 6 8 8 4
+
+
+Sequence : 22ak93c1.r1ta
+Is_read
+Padded
+Staden_id 12
+Clipping QUAL 31 335
+Align_to_SCF 1 1315 1 1315
+SCF_File 22ak93c1.r1taSCF
+Primer Universal_primer
+Strand Reverse
+Dye Dye_terminator
+Template 22ak93c1
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 30 "M13mp18"
+Tag ALUS 31 172
+Tag DONE 150 150 "AUTO-EDIT: replaced T by a at 150 (terminator, isolated, strong)"
+Tag DONE 178 178 "AUTO-EDIT: replaced G by t at 178 (terminator, isolated, strong)"
+Tag DONE 188 188 "AUTO-EDIT: replaced T by a at 188 (terminator, isolated, strong)"
+Tag DONE 230 230 "AUTO-EDIT: replaced T by g at 230 (terminator, isolated, strong)"
+Tag DONE 261 261 "AUTO-EDIT: replaced C by g at 261 (terminated, isolated, strong)"
+Tag ALUS 262 446
+Tag DONE 305 305 "AUTO-EDIT: replaced T by g at 305 (terminated, isolated, strong)"
+Tag DONE 310 310 "AUTO-EDIT: inserted g at 310  (terminated, compound, strong)"
+Tag DONE 325 325 "AUTO-EDIT: replaced A by g at 325 (terminated, isolated, strong)"
+
+DNA : 22am91h5.r1t
+GGGnnATAGATACGATCCGAGCTCGGTACCCATGTTGGCCAAGCTGGTCTCGAGCTCCTG
+ACCTAGGATTACAGGCCTAAGCCACCGCACCCGGCATGATGGGTCTTTATTCTTCAAAGC
+AGGAGGAAGGGATCCTAGAAAAACAGAGACAAGGCCAAACATGGTAGCTCACACCTGTAA
+TCCCAGCACTTTGGGAGGCCAGTGCGGGTGAATCACGAAGTCAGGAGTTCAAGACCAGCC
+TGGCCAACATGGTGAAACACCGTCTCTACTAAAATAAAAAGAAATTAGCTGGGTGTCGTG
+GCAGGTGCCTGTAATCCCAGCCACTTGGGAAGCTGAGGCAGGAGAATCGCTTGAACCCGG
+GAGGTGGAGGTTGCAnTGAGCCGAGATCACGCGACTGCACTCCAGCCCAACCAATAATGT
+GAGACTCTGTCTCGAnAAnnnAAAGCnGAAAACAGACnnCTAGTACnGTACTTAACGGGG
+TAATTATAATAATTAAATGAAAAAAATAnCTGTGAGGTGATTGATATAnTGCTGTTGCTA
+AATACAACTAACATTTTAATATACCGGTGGAGTGTTTCTAATCTGAnAATCCAAATTAGA
+AATGCTCACAGTCTGAAACTTTTTGAGCACCAACTAATGTTCAAAGAATTGCTATGGACC
+ATATGGGTGGTTGATTTT
+
+BaseQuality : 22am91h5.r1t
+4 8 4 4 4 4 4 7 10 9 10 15 10 7 7 10 10 9 19 20 33 34 34 34 36 36 33
+33 31 33 33 32 31 31 26 26 27 23 30 26 26 26 26 34 34 38 38 38 37 45
+45 37 37 37 37 45 45 45 45 37 37 37 45 45 32 18 10 21 18 28 29 33 33
+33 33 45 45 42 42 37 33 33 33 36 33 33 30 30 30 30 32 36 33 33 33 33
+33 33 37 37 45 45 37 37 37 33 33 35 35 35 35 43 35 42 42 35 35 34 34
+34 29 29 22 27 27 27 37 27 37 45 33 30 30 37 28 32 32 10 10 21 32 32
+34 27 23 12 11 9 9 9 18 21 28 31 33 30 30 30 30 34 34 34 34 31 33 34
+34 33 31 33 33 33 33 33 33 45 45 40 37 37 37 33 33 30 30 30 30 33 30
+33 26 26 26 25 23 18 14 12 20 23 27 25 21 21 21 28 28 30 31 31 31 31
+32 21 21 12 6 6 9 9 14 17 19 17 10 16 13 8 8 11 13 9 13 12 14 14 21 23
+26 34 36 34 31 22 24 20 28 28 33 27 28 25 27 24 34 32 23 12 12 12 23
+23 33 28 34 34 37 32 32 15 11 11 32 32 40 37 38 18 11 9 9 18 25 45 34
+28 28 9 9 20 29 45 38 34 23 23 10 23 23 33 33 33 21 21 13 25 25 25 29
+27 33 30 33 33 34 33 30 30 19 19 30 30 30 26 26 30 27 27 23 19 15 24
+27 28 21 9 9 9 19 14 12 10 12 12 13 17 28 18 19 21 24 24 25 24 24 24
+24 18 18 13 18 8 8 8 10 10 10 7 7 7 8 8 12 15 4 4 4 25 18 21 17 15 16
+14 9 9 17 14 17 9 17 8 8 10 10 10 11 17 17 22 24 23 24 24 19 13 10 8
+15 15 10 9 9 9 16 12 12 14 19 18 26 24 22 22 24 25 21 24 24 32 19 20 9
+9 4 4 4 4 4 4 4 4 17 16 16 4 4 4 7 10 8 7 6 13 8 8 4 4 4 4 8 8 7 7 7 4
+4 4 17 23 12 9 8 7 7 8 7 10 10 8 6 6 6 6 6 10 10 7 7 7 7 9 7 8 10 7 7
+8 6 6 8 8 8 9 10 7 12 4 4 4 9 9 7 6 6 7 6 6 10 9 7 8 10 7 7 7 9 4 4 4
+14 8 8 9 7 6 6 8 8 8 7 7 6 6 9 7 7 10 13 8 9 8 8 8 14 7 7 7 7 9 8 12
+12 4 4 4 7 7 7 7 8 8 8 7 7 7 7 7 7 8 10 8 6 6 6 4 4 4 8 12 9 9 17 9 7
+7 7 7 6 6 6 6 8 10 6 6 6 8 6 6 6 6 6 6 6 6 6 6 6 6 6 13 11 9 8 7 8 7 7
+7 8 7 9 8 7 6 6 4 6 6 8 7 7 7 6 8 6 6 8 7 7 9 9 10 11 7 6 6 6 6 6 12 9
+8 8 12 11 10 8 7 7 4 4 4 7 7 8 4
+ 
+
+Sequence : 22am91h5.r1t
+Is_read
+Padded
+Staden_id 20
+Clipping QUAL 32 361
+Align_to_SCF 1 678 1 678
+SCF_File 22am91h5.r1tSCF
+Primer Universal_primer
+Strand Reverse
+Dye Dye_terminator
+Template 22am91h5
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 31 "M13mp18"
+Tag ALUS 153 443
+
+DNA : 22aq40a8.s1
+AAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCACAGAGACAAGGCCAAACATGG
+TAGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAGTGCGGGTGAATCACGAGGTCAG
+GAGTTCAAGACCAGCCTGGCCAACATGGTGAA-CACCGTCTCTACTAAAATAAAAAGAAA
+TTAGCTGGGTGTCGTGGCAGGTGCTtGTAATCCCAGCCACTTGGGAAGCTGAGGCAGGAG
+AATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCGACTGCACTCCA
+GCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGACAAGACAACTAG
+TACAGTACTTACAGGGTTATTATGATGATTAnATGAGAGAATAGCTGTGAGGTGATTGAT
+ATAGTGCTGTGCTTAAATACAACTATCAATTTTATTATACGGGGTTGAGTGTTTCCAATC
+TGAAAATCCCAAATTTGGAATGCTCTACGGTCTGAAACCTTTTTTGAGCACCGACCTAAA
+
+BaseQuality : 22aq40a8.s1
+7 9 10 11 11 23 27 25 25 9 9 9 12 14 23 27 24 21 21 16 22 22 22 24 23
+23 23 30 30 30 30 30 32 34 31 31 34 34 28 28 28 30 30 30 28 28 28 34
+34 34 34 34 34 37 34 34 34 34 34 34 37 37 37 37 37 37 37 32 34 29 29
+29 45 38 40 40 37 37 32 37 37 37 37 37 37 37 37 37 40 40 40 40 40 37
+30 30 32 22 22 22 32 32 41 45 45 41 41 45 30 32 27 32 32 40 37 37 40
+37 37 45 45 45 40 40 37 37 37 37 37 37 37 37 40 37 37 37 31 32 28 28
+28 32 32 37 34 37 38 38 38 27 27 11 1 11 10 21 27 34 34 37 37 34 34 45
+45 45 38 37 37 37 37 37 37 34 34 34 34 33 30 30 28 28 28 28 28 30 37
+37 37 37 33 36 36 31 31 31 24 24 24 24 21 21 17 12 4 4 4 14 17 26 25
+25 31 34 34 31 31 31 33 31 31 34 35 37 37 32 32 34 34 30 31 31 34 30
+33 30 30 30 33 33 30 30 23 22 22 32 32 40 34 36 34 31 26 28 26 24 16 9
+9 9 8 15 22 22 25 25 31 31 30 30 28 30 30 30 30 28 28 28 30 30 30 30
+30 37 32 27 23 18 18 27 27 34 34 31 33 30 30 28 28 28 28 28 30 34 33
+33 33 33 30 26 26 23 23 23 30 37 36 32 32 24 23 21 20 28 31 29 30 22
+21 18 13 15 23 26 34 34 36 33 30 30 30 30 25 25 25 26 23 26 25 24 24
+25 27 25 22 14 15 12 12 12 21 21 25 25 22 21 21 24 19 19 14 16 11 13
+14 20 27 30 28 28 28 30 33 34 34 23 23 16 7 7 4 4 4 18 17 21 23 21 17
+11 13 18 18 18 20 18 18 11 14 7 7 7 15 20 18 11 10 15 23 18 16 10 8 8
+13 17 18 14 14 14 13 13 14 17 18 13 13 9 9 9 14 12 18 18 11 11 5 5 5 6
+6 6 8 12 10 13 15 11 11 11 8 6 6 6 6 6 8 6 6 8 8 8 7 7 9 9 8 8 19 8 8
+14 8 8 6 6 11 9 9 8 14 6 6 7 7 8 6 7 6 6 9 9 10 15 14 12 7 7 7 7 13 15
+22 22 19 14 9 8 8 8 12 15 19 7 6 10 9 8 6 7 9 9 10 10 8 8 7 7 4 4
+
+
+Sequence : 22aq40a8.s1
+Is_read
+Padded
+Staden_id 3
+Clipping QUAL 39 388
+Align_to_SCF 1 152 1 152
+Align_to_SCF 154 540 153 539
+SCF_File 22aq40a8.s1SCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_primer
+Template 22aq40a8
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 38 "M13mp18"
+Tag ALUS 49 339
+Tag DONE 206 206 "AUTO-EDIT: replaced N by t at 206 (terminated, compound, strong)"
+
+DNA : 22an02b10.s1t
+CGTGCCTGCAGGTCGACTCTAGAGGATCCCCCCTGTAATCCCAGCACTTTGGGAGGCCAg
+TGCGGGTGAATCACgATGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACACCG
+TCTCTACTAAAATAAAAAGAAATTAGCTGGGTGTCgTGGCAGGTGCCTGTAATCCCAGCC
+ACTTGGGAAGCTGAgGCAGGAGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCC
+GAgATCACGCGACTGCACTCCAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAA
+AAAGCAGAGACAAGACAACTAGTACAGTACTTACaGGGTTATTATGATGATTAAATGAGA
+gAATAGCTGTGAgGTGATTGATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATA
+CGGGTTGAGTGTATCTAATCTGAAAATCCAAAATCAGAAATGCGCTACAGTCTGAAACTT
+TTTTGAGCACCGACCTAATGTTCCAAGGAAGTGCTTATCGGAGCACCATGGGTCGGTACA
+CATTTGGGTCGGGAATATnCCACCAGTGAGTACCATAAAATGCCAATATCCCCTCCCACC
+CCCAACA
+
+BaseQuality : 22an02b10.s1t
+7 7 9 12 10 15 16 17 13 13 21 18 23 17 28 28 37 37 37 33 26 26 17 19
+12 21 25 36 45 45 34 34 34 34 34 37 37 37 37 37 32 25 9 9 13 17 17 14
+18 18 21 29 32 19 18 18 18 21 9 9 9 23 19 30 30 37 29 37 37 32 34 23
+20 13 13 10 13 22 32 21 17 17 23 18 10 10 10 12 13 9 9 14 23 29 28 22
+22 32 33 37 34 34 34 34 34 34 34 30 37 32 37 37 41 34 33 33 33 21 21
+14 14 14 24 28 41 37 32 32 21 21 21 25 30 30 37 30 37 30 30 30 32 36
+28 27 17 27 27 37 32 37 36 37 25 23 10 10 10 32 21 23 10 15 10 23 23
+36 32 32 34 34 34 37 37 25 25 13 9 9 21 25 25 32 32 32 41 32 28 28 11
+22 22 32 32 13 13 10 11 9 10 10 12 12 14 32 32 29 22 22 21 21 17 21 27
+41 41 41 28 27 32 27 27 29 37 32 34 17 15 14 28 23 25 20 9 9 9 25 10
+12 12 14 13 10 10 9 16 13 23 23 14 14 22 25 34 28 28 22 28 28 28 19 18
+10 10 11 19 21 28 28 28 30 30 30 23 18 10 10 28 32 32 34 28 28 28 28
+28 31 34 34 25 25 12 19 11 14 14 25 21 24 24 18 18 18 22 23 13 11 10
+10 10 10 14 13 10 9 9 7 8 14 17 16 10 10 13 16 15 18 18 18 18 14 15 12
+12 9 12 9 12 12 13 9 14 10 18 19 14 9 13 11 18 14 15 15 16 20 19 18 14
+9 8 8 6 6 6 11 9 9 14 17 20 12 13 6 6 8 8 11 10 21 23 15 14 13 13 14
+21 27 28 32 29 34 28 27 23 14 10 10 12 17 15 13 11 11 11 18 28 33 24
+18 14 12 17 12 12 18 22 19 19 24 28 20 21 19 19 20 17 17 26 25 20 9 14
+7 7 7 15 21 33 27 27 18 20 19 15 15 20 18 14 14 8 8 9 9 9 7 7 7 7 7 8
+9 9 12 7 7 9 11 10 11 13 13 11 7 7 8 15 17 26 25 25 27 25 24 21 19 20
+18 13 13 14 9 9 14 18 17 12 15 16 21 20 18 8 7 6 6 6 13 20 25 18 11 14
+9 9 9 11 11 12 12 15 15 18 10 10 8 8 10 10 9 9 9 16 11 7 7 6 6 8 4 4 4
+4 4 6 10 10 13 13 12 6 6 6 6 12 15 12 11 11 4 4 4 6 6 6 6 6 8 6 6 6 8
+6 8 11 13 8 8 6 6 6 6 9 16 9 13 13 17 14 13 9 7 7 12 8 8 8 9 9 9 9 9 9
+8 8 8 10 9 7
+
+
+Sequence : 22an02b10.s1t
+Is_read
+Padded
+Staden_id 21
+Clipping QUAL 32 446
+Align_to_SCF 1 607 1 607
+SCF_File 22an02b10.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22an02b10
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 31 "M13mp18"
+Tag ALUS 32 301
+Tag DONE 60 60 "AUTO-EDIT: inserted g at 60  (terminated, compound, strong)"
+Tag DONE 75 75 "AUTO-EDIT: replaced A by g at 75 (terminated, isolated, strong)"
+Tag DONE 156 156 "AUTO-EDIT: replaced C by g at 156 (terminated, isolated, strong)"
+Tag DONE 195 195 "AUTO-EDIT: replaced A by g at 195 (terminated, isolated, strong)"
+Tag DONE 243 243 "AUTO-EDIT: replaced T by g at 243 (terminated, isolated, strong)"
+Tag DONE 335 335 "AUTO-EDIT: replaced T by a at 335 (all_stranded, isolated, strong)"
+Tag DONE 361 361 "AUTO-EDIT: replaced C by g at 361 (terminated, isolated, strong)"
+Tag DONE 373 373 "AUTO-EDIT: replaced C by g at 373 (terminated, isolated, strong)"
+
+DNA : 22ak67d6.r1t
+CGCGCCCCTCnGACTCCGGCCCTAnCACGGTACCCACTTTGGGAGGCCAGTGCGGGTGAA
+TCACGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACACCGTCTCTACTAA
+AATAAAAAGAAATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAGCCACTTGGGAAG
+CTGAgGCAGGAGAATCGCTTGAACCCGGGAgGTGGAGGTTGCAGTGAGCCGAGATCACGC
+GACTGCACTCCAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGA
+TCAGATCACTAGTACAGTACTTACGGGCTTATTATGATGATTAAATGAGAGAATACCTGT
+GAGGTGATTGATATATTGCTGTGCTTAATACAnACTATCATTTTATTATACGGGTCGAGT
+GTTTCTAATCTGACAATCCAAATTAGAAATGCTCTACAGTCTGAAACCTTTTTGAGCACC
+CACCTACTGTTCAAAGGACGTGCTTATTGGAGCATTATGGGTTGTAACATTTTTGGGTTG
+GGAATTTTCACCCATTAGTACTATAAATGCCATTTTCCAAAAAATCTGAATCTAAAATTT
+CTGGTCCTACCAGCATTTGCTAGGGATCCCACCCGTCTTCTTCCTTACCTGTTTAAGTCT
+TATAAATC
+
+BaseQuality : 22ak67d6.r1t
+6 6 6 4 8 11 15 8 4 4 4 4 8 6 6 9 7 8 9 8 4 4 4 4 4 4 10 9 9 21 19 33
+30 33 30 30 30 28 28 28 23 23 20 11 11 16 12 13 17 21 25 28 33 33 33
+38 34 34 34 33 30 22 22 12 14 21 10 12 21 21 16 14 11 13 13 14 21 21
+21 19 15 18 23 23 25 30 28 33 38 38 33 33 30 30 30 30 30 30 30 37 37
+45 45 33 33 33 33 33 33 40 42 49 42 40 40 40 34 34 31 35 34 35 37 42
+42 37 32 19 9 9 11 23 19 28 30 30 28 38 45 37 37 37 32 32 24 34 34 30
+30 26 21 20 11 27 27 40 34 30 30 28 30 30 30 23 18 17 9 9 18 16 16 24
+24 24 23 21 18 11 11 18 25 25 16 9 7 9 15 11 8 7 9 7 7 8 16 16 27 28
+25 20 16 10 10 9 13 8 8 7 12 4 4 4 14 6 6 6 7 9 6 7 7 7 7 16 10 20 15
+13 11 9 9 8 8 8 15 8 10 10 17 14 9 9 19 15 15 7 7 12 20 19 25 25 22 18
+17 7 9 12 20 21 25 21 21 20 22 28 32 30 25 25 12 12 18 32 32 36 49 49
+33 33 24 20 13 12 16 18 20 14 25 23 25 19 12 11 8 8 4 4 4 4 4 8 8 6 4
+6 8 15 17 21 19 15 8 8 8 8 10 7 7 13 9 7 11 14 6 6 4 4 4 6 12 8 13 17
+21 18 19 19 20 18 18 15 10 15 14 13 14 8 8 8 9 8 8 10 10 8 8 15 17 17
+17 17 14 13 19 17 16 12 10 14 15 17 15 14 8 8 7 7 15 19 18 12 11 16 12
+15 11 10 13 13 11 4 4 4 10 12 12 18 12 14 9 10 9 10 21 22 18 14 6 6 6
+6 6 8 4 4 4 8 8 7 8 7 9 14 7 8 7 10 16 31 17 14 4 4 4 10 11 8 10 13 17
+13 8 8 9 8 8 12 12 14 14 7 7 7 7 7 7 7 8 9 13 11 10 7 10 7 6 6 10 8 8
+23 23 12 7 7 7 7 7 10 10 18 6 6 6 6 6 6 7 7 7 6 6 6 7 6 6 4 4 4 4 8 11
+18 15 23 11 9 8 7 7 7 7 7 7 8 15 18 8 10 10 12 8 6 8 6 6 6 7 7 12 10
+12 18 11 12 10 19 19 15 15 10 10 9 7 11 7 8 9 10 7 7 6 6 6 6 4 6 7 7 7
+7 9 9 9 13 13 9 7 7 7 9 7 7 7 6 6 6 6 8 8 9 8 11 8 7 6 6 6 6 9 7 8 7 7
+6 8 6 6 9 10 12 10 13 12 6 6 6 6 6 7 6 6 7 7 7 11 13 11 9 8 6 6 8 6 6
+8 12 14 8 8 8 6 13 8 8 8 6 7 8 7 8 9 8 10 6 6 10 11 17 20 16 11 11 7 6
+8 8 9 8 6 12 10 7 7 8 8 4
+
+
+Sequence : 22ak67d6.r1t
+Is_read
+Padded
+Staden_id 19
+Clipping QUAL 30 292
+Align_to_SCF 1 668 1 668
+SCF_File 22ak67d6.r1tSCF
+Primer Universal_primer
+Strand Reverse
+Dye Dye_terminator
+Template 22ak67d6
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Tag ALUS 35 291
+Tag DONE 185 185 "AUTO-EDIT: replaced C by g at 185 (terminated, isolated, strong)"
+Tag DONE 211 211 "AUTO-EDIT: replaced T by g at 211 (terminated, isolated, strong)"
+
+DNA : 22ak64b1.s1t
+TGGGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAACACCGTCTCTACTAAA
+ATAAAAAGAAATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAGCCACTTGGGAAGC
+TGAGGCAGGAGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAgATCACGCG
+ACTGCACTCCAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGAC
+AAGAcCACTAGTACAGTACTTACAGGGTtATTATGATGATTAAATGAGAGAATAGCTGTG
+AGGTGATTGATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATACGGGTTGAGTG
+TTTCTAATCTGAaAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACC
+GACCTAAATGTTCAAAGGGAGGTGCTTAATTGGAGCATTATGGGTTGTTAGATCTTTGGG
+TTGGGAATATTCAACC
+
+BaseQuality : 22ak64b1.s1t
+4 4 8 11 13 20 16 16 16 19 24 32 31 37 37 35 34 29 32 18 32 24 33 33
+37 37 37 23 32 11 10 21 17 37 34 34 45 45 45 45 32 29 26 26 28 30 30
+33 45 45 49 49 45 43 45 45 45 45 45 37 45 45 45 45 45 45 45 37 37 37
+38 32 40 37 41 37 45 32 45 45 44 37 45 30 45 45 33 33 33 33 33 22 37
+37 45 45 45 40 37 34 37 37 33 32 32 33 33 33 33 40 40 40 42 40 42 37
+37 37 45 45 45 45 45 35 34 34 28 28 18 21 17 17 27 38 38 45 34 34 34
+34 34 34 34 34 34 34 34 34 34 34 34 37 34 34 33 33 33 33 28 31 31 36
+34 31 36 36 34 33 20 14 8 8 8 8 14 23 27 31 36 33 36 38 30 30 28 28 28
+30 30 27 30 30 30 30 33 33 23 21 18 18 17 20 32 37 37 45 45 45 45 34
+34 34 45 45 45 44 45 37 45 45 45 37 40 37 37 33 40 44 40 40 36 31 31
+28 25 22 27 14 14 8 9 10 8 7 8 7 12 18 18 23 15 9 9 15 15 20 20 25 28
+25 21 21 18 18 8 9 8 6 6 8 11 14 28 28 28 30 30 30 30 31 30 31 25 22
+22 27 15 15 15 16 13 17 25 25 30 25 27 28 31 24 24 19 24 29 33 27 31
+31 31 31 28 27 22 14 15 27 32 40 37 36 36 36 33 33 34 33 27 13 14 7 7
+8 18 23 32 32 23 20 20 33 30 27 27 21 14 14 18 21 18 21 15 15 17 11 11
+16 24 23 30 23 14 10 13 14 15 10 8 9 16 16 28 4 4 4 10 11 12 9 8 10 14
+20 18 19 19 19 10 10 12 15 25 23 26 26 28 18 14 14 16 20 15 14 12 12
+12 21 23 25 24 21 17 17 14 21 20 19 12 9 9 8 9 8 10 7 6 9 8 7 8 9 7 7
+7 6 6 8 7 8 6 6 6 6 6 13 11 6 6 6 8 10 11 8 9 9 10 10 15 10 11 12 16
+13 10 9 7 7 7 7 9 10 8 8 7 6 6 6 6 6 14 10 7 7 7 9 9 10 11 16 16 7 6 4
+4 4 8 4
+
+
+Sequence : 22ak64b1.s1t
+Is_read
+Padded
+Staden_id 8
+Clipping QUAL 40 416
+Align_to_SCF 1 496 1 496
+SCF_File 22ak64b1.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak64b1
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 39 "M13mp"
+Tag CONF 44 44 "AUTOEDIT: possible compression"
+Tag CONF 96 96 "AUTOEDIT: possible compression"
+Tag DONE 172 172 "AUTO-EDIT: replaced A by g at 172 (terminated, isolated, strong)"
+Tag DONE 245 245 "AUTO-EDIT: replaced A by c at 245 (all_stranded, compound, strong)"
+Tag AMBG 246 246 "AUTOEDIT: Check this edit stack (conflict & possible misassembly))!"
+Tag DONE 269 269 "AUTO-EDIT: replaced C by t at 269 (all_stranded, isolated, strong)"
+Tag DONE 373 373 "AUTO-EDIT: replaced N by a at 373 (terminated, isolated, strong)"
+
+DNA : 22ak81h10.s1t
+GTGGCATGCCTGCAGGTCAACTCTAAAAGATCCCCTGAAACACCGTCTCTACTAAAATAA
+AAAGAAATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAgCCACTTGGGAAGCTGAG
+GCAGGAGAATCGCTTGAACCCGGGAgGTGGAgGTTGCAGTGAGCCGAGATCACGCGACTG
+CACTCCAGCCCAAcCAATAGTGTGAGACTCTGTCTCGAaaAAaAAAAAGCAgAGACaAGA
+CCACTAGTACAGTACTTACAGGGTTATTATGATGATTAAaTGAGAGAATAGCTGTGAGGT
+GATTGATATAGTGCTGTGCTTAATACaAACTATCATTTTATTATACGGGTTGAGTGTTTC
+TAATCTGAAAATCCaaAATTAGAAATGCTCTACAGTCTGAAACTTTtTTGAGCACCGACC
+TACTGTGCACAGGAAGTGCTTATTGGAGCATGATGGGTTGTTACATATTTGGGTTGGGAA
+TATTCACCCAGTAAGTACTATAAAATGCAAATATTCCAACAAG
+
+BaseQuality : 22ak81h10.s1t
+6 6 6 6 6 8 9 16 11 13 21 20 22 22 22 15 14 9 9 9 9 15 18 15 9 9 9 9 9
+15 23 36 36 36 36 34 38 40 30 28 28 28 30 32 37 37 36 38 37 44 45 45
+49 49 45 45 37 37 38 45 45 45 38 37 37 38 38 37 37 34 34 34 32 32 38
+45 49 45 45 45 45 37 33 33 27 27 18 18 30 32 36 36 36 36 36 36 36 34
+32 21 18 4 4 4 18 17 27 28 33 36 36 36 49 36 36 36 34 34 45 27 27 30
+25 27 12 20 20 34 34 38 37 37 34 34 25 25 18 18 21 29 38 45 21 28 14
+14 14 27 21 34 18 17 17 32 27 25 22 13 13 10 18 15 32 32 32 37 28 22
+22 21 14 10 10 11 23 22 28 20 14 14 8 8 11 20 20 16 12 16 16 28 21 19
+10 10 10 19 18 20 20 14 14 32 32 34 32 25 18 18 18 18 27 32 32 24 24
+23 23 14 10 10 10 9 9 10 11 12 12 12 12 17 11 11 11 10 8 6 6 8 8 10 16
+16 16 11 14 10 9 9 9 17 11 11 11 14 15 22 18 14 14 11 11 15 27 22 19
+23 27 30 28 22 18 16 11 10 10 11 9 10 11 11 12 15 20 27 27 23 21 14 10
+10 10 10 14 22 25 22 14 14 22 32 38 34 34 34 31 24 18 14 12 9 10 10 23
+18 24 16 16 18 18 18 19 18 17 21 18 18 12 14 21 27 37 34 34 28 28 34
+27 27 28 31 20 18 11 9 9 12 11 11 27 15 15 20 23 26 14 8 8 8 8 14 16
+23 19 19 28 31 31 31 25 18 11 11 18 10 10 10 10 11 12 16 16 21 23 12
+12 13 23 13 13 19 11 10 10 10 10 10 18 21 28 16 19 29 32 19 19 8 8 8
+10 10 18 18 22 21 16 18 22 21 11 11 14 14 15 14 18 7 7 7 7 7 7 14 18
+10 10 9 9 14 15 15 23 20 22 19 19 21 16 11 11 9 8 8 9 9 18 14 11 9 9
+15 8 9 10 8 9 8 8 8 14 14 25 27 16 9 8 14 8 9 17 13 18 15 8 8 7 7 7 7
+6 6 6 6 6 4 4 4 8 9 10 17 11 15 12 17 9 9 10 7 7 7 10 11 10 7 7 7 6 6
+8 6 6 8 4
+
+
+Sequence : 22ak81h10.s1t
+Is_read
+Padded
+Staden_id 5
+Clipping QUAL 36 418
+Align_to_SCF 1 523 1 523
+SCF_File 22ak81h10.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak81h10
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 35 "M13mp1"
+Tag ALUS 36 216
+Tag DONE 103 103 "AUTO-EDIT: replaced N by g at 103 (terminated, isolated, strong)"
+Tag DONE 146 146 "AUTO-EDIT: replaced T by g at 146 (terminated, isolated, strong)"
+Tag DONE 152 152 "AUTO-EDIT: replaced A by g at 152 (terminated, isolated, strong)"
+Tag DONE 194 194 "AUTO-EDIT: replaced G by c at 194 (all_stranded, isolated, strong)"
+Tag DONE 219 219 "AUTO-EDIT: replaced G by a at 219 (all_stranded, compound, strong)"
+Tag DONE 220 220 "AUTO-EDIT: replaced C by a at 220 (all_stranded, compound, strong)"
+Tag DONE 223 223 "AUTO-EDIT: replaced G by a at 223 (all_stranded, isolated, strong)"
+Tag DONE 232 232 "AUTO-EDIT: replaced C by g at 232 (all_stranded, isolated, strong)"
+Tag DONE 237 237 "AUTO-EDIT: replaced G by a at 237 (all_stranded, isolated, strong)"
+Tag DONE 280 280 "AUTO-EDIT: replaced G by a at 280 (terminated, isolated, strong)"
+Tag DONE 327 327 "AUTO-EDIT: replaced G by a at 327 (terminated, isolated, strong)"
+Tag DONE 375 375 "AUTO-EDIT: replaced T by a at 375 (terminated, compound, strong)"
+Tag DONE 376 376 "AUTO-EDIT: replaced C by a at 376 (terminated, compound, strong)"
+Tag DONE 407 407 "AUTO-EDIT: replaced C by t at 407 (terminated, isolated, strong)"
+
+DNA : 22ak66c6.s1t
+nCGTGGCTGCCTGCAGGTCGACTCTAGAGGATCCCCACCGTCTCTACTAAAATAAAAAGA
+AATTAGCTGGGTGTCGTGGCAGGTGCCTGTAATCCCAGCCACTTGGGAAGCTGAGGCAGG
+AGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCGACTGCACTC
+CAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGACAAGACAACT
+AGTACAGTACTTACAGGGTTATTATGATGATTAAATGAGAGAATAGCTGTGAGGTGATTG
+ATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATACGGGTTGAGTGTTTCTAATC
+TGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAATG
+TTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGGGTTGGGAATATTC
+AACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAATCTGAAACATtTC
+TGGTCCTAAGCAAGCATnTTGCAAAGGGATACGCAACCTGTAGTACGTCCTTTATCATTG
+TnTCAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAnCAnATTCGATTGTA
+TTATT
+
+BaseQuality : 22ak66c6.s1t
+4 4 8 4 6 10 13 21 24 25 33 33 33 30 27 21 15 27 19 30 30 33 33 30 21
+21 10 9 17 11 27 27 37 38 33 35 35 35 35 44 45 45 38 37 37 45 45 45 43
+43 43 45 45 45 45 45 45 45 21 21 23 30 30 34 37 37 38 45 32 45 37 41
+37 45 30 45 45 34 34 34 32 29 22 32 32 45 45 45 33 28 28 37 37 34 34
+34 35 34 34 34 34 34 34 37 37 40 41 37 34 37 37 37 32 24 22 27 29 25
+27 20 21 21 21 27 45 41 40 40 40 42 34 34 37 41 45 51 45 45 37 30 37
+41 41 37 36 28 30 30 30 22 33 33 35 33 33 41 51 51 45 39 39 39 30 28
+33 34 34 39 34 41 34 34 41 33 33 39 39 39 33 33 33 30 33 33 33 30 29
+19 19 24 25 32 33 45 36 36 36 36 41 41 30 30 30 37 37 43 51 51 51 51
+45 37 37 30 37 37 37 51 51 51 51 51 37 37 28 28 10 10 10 13 10 10 10 9
+9 9 21 21 28 37 37 37 33 33 33 33 33 33 34 34 34 33 33 33 33 21 25 25
+22 22 29 29 33 33 33 33 31 31 27 28 17 23 23 28 26 24 32 10 10 10 15
+15 26 32 32 37 45 45 32 45 33 26 24 32 37 37 35 34 37 35 37 34 37 37
+40 37 37 45 45 38 38 45 45 40 37 37 37 34 30 30 28 28 28 30 37 37 34
+34 34 34 34 33 31 31 25 21 19 22 21 25 30 32 32 30 22 22 25 28 24 25
+26 17 17 23 27 27 33 31 31 34 38 38 38 38 34 26 17 15 8 8 11 16 25 31
+32 33 29 26 32 29 33 34 34 34 36 33 34 34 31 27 31 34 38 45 45 36 37
+37 37 36 36 38 34 32 28 25 22 22 21 17 18 29 29 23 23 22 27 25 20 18
+11 11 15 15 16 23 21 17 19 24 24 24 31 31 31 33 33 33 31 33 33 29 29
+29 29 29 31 31 31 23 24 19 12 10 16 10 10 20 10 10 13 15 30 23 29 23
+28 18 10 10 16 21 18 19 19 24 24 12 11 9 9 10 21 23 31 31 33 28 28 14
+17 17 28 21 30 24 30 28 22 26 23 19 10 10 12 23 22 23 19 12 10 9 9 10
+19 24 29 30 34 34 34 34 34 29 25 25 32 26 31 31 32 20 17 15 12 12 4 4
+4 17 23 33 30 24 18 13 18 16 12 16 8 9 9 19 15 15 4 4 4 7 11 6 6 6 7 8
+9 17 12 13 19 23 25 13 13 13 19 21 29 32 30 26 20 20 12 9 9 8 9 8 8 15
+24 17 15 8 8 9 17 16 4 4 4 8 8 13 15 23 14 9 8 8 10 10 17 23 21 17 12
+13 22 20 19 15 11 11 9 8 8 8 8 8 8 10 9 9 7 8 8 10 12 10 10 12 4 4 4 4
+4 4 10 10 10 10 10 9 8 8 7 8 9 11 9 9 4
+
+
+Sequence : 22ak66c6.s1t
+Is_read
+Padded
+Staden_id 18
+Clipping QUAL 37 548
+Align_to_SCF 1 665 1 665
+SCF_File 22ak66c6.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak66c6
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 36 "M13mp"
+Tag ALUS 38 221
+Tag DONE 538 538 "AUTO-EDIT: replaced N by t at 538 (terminated, isolated, strong)"
+
+DNA : 22ak93c1.s1lt
+TTTCTTTGGGGGCAATCTTGATGCTAGCGTCGTTCAAGATGATCCACACCCAGCTCACTG
+TTTTATTCTCTCCAATAATCCTAAGCAAGCTCTCAAAATATAAATATGCTTTTTAATAAT
+ACAATCGAATTTGCTATACCACCTCAGAATCTGTACCACAATATATTAACTACTTAAAAC
+AATGATAAAGAACGTACTACAGGTTGCGTATCCCTTTGCAAAATGCTTGCTTAGGACCAG
+AAATGTTTCAGATTTCAGATTTTTTTTGGAATATTTGCATTTTATAGTACTTACTGGTTG
+AATATTCCCAACCCAAAAATCTAACAACCCATAATGCTCCAATAAGCACTTCCTTTGAAC
+ATTAGGTCGGTGCTCAAAAAAGTTTCAGACTGTAGAGCATTTCTAATTTTGGATTTTCAG
+ATTAGAAACACTCAACCCGTATAATAAAATGATAGTTTGTATTAAGCACAGCACTATATC
+AATCACCTCACAGCTATTCTCTCATTTAATCATCATAATAACCCTGTAAGTACTGTACTA
+gTTGTCTTGTCTCTGCTTTTTTTTTTTCgAgACAGAGTCTCACACTATTGGTTGGGCTGG
+AATGCAnTCGCGTGATCTCGGCTCACTGGCAACCTCCACCTCCCGGGG
+
+BaseQuality : 22ak93c1.s1lt
+4 4 4 4 4 4 4 4 9 6 4 8 15 11 4 6 4 12 8 9 10 12 11 8 8 6 7 8 14 14 9
+4 4 8 8 8 8 8 4 6 8 11 9 10 8 9 9 11 10 9 9 9 9 9 9 16 16 32 23 29 21
+27 27 22 22 20 28 32 37 37 34 31 30 28 28 24 29 29 34 34 38 32 32 18
+22 18 22 22 28 29 37 37 37 37 35 35 34 34 35 34 37 37 37 37 37 37 34
+37 37 37 37 32 32 28 28 31 30 30 30 23 23 23 28 27 21 32 13 13 16 24
+32 34 31 30 28 28 28 28 28 34 34 34 34 33 25 16 9 9 10 23 32 33 41 37
+34 28 30 30 30 28 28 22 27 25 32 25 30 27 22 24 22 22 28 25 24 30 30
+30 30 30 30 24 28 13 10 10 23 20 18 9 9 9 18 23 30 30 30 28 24 19 25
+11 11 12 27 31 34 30 30 30 30 37 45 51 41 39 28 28 28 25 25 24 30 34
+39 39 39 39 39 39 36 31 13 10 9 10 10 23 23 9 9 13 23 31 31 14 14 31
+31 13 9 9 9 23 23 23 13 9 9 9 31 31 41 51 36 31 14 13 31 19 30 31 31
+22 34 25 33 33 33 30 24 29 30 18 24 24 11 11 24 24 33 39 34 34 30 30
+34 22 22 14 19 18 23 23 17 31 24 37 33 33 33 33 33 28 34 18 18 27 34
+34 38 27 20 20 24 24 30 30 39 34 34 30 22 22 24 33 33 33 33 28 24 24
+22 30 22 24 24 33 33 33 39 51 51 41 32 27 17 25 17 31 31 34 19 15 10
+10 31 14 27 21 30 30 24 24 30 34 39 39 41 36 34 30 30 30 34 31 16 9 9
+10 20 19 16 11 9 9 9 13 23 22 28 28 37 33 33 33 28 32 18 13 10 20 18
+10 32 21 31 23 14 9 9 9 16 11 9 9 10 20 25 33 30 30 28 32 28 28 37 35
+35 34 34 34 34 34 37 40 37 33 32 25 12 10 10 16 16 28 32 32 34 30 28
+28 29 31 33 32 28 30 30 25 21 16 19 19 19 27 19 14 14 22 22 28 25 29
+29 29 30 30 23 23 21 18 14 21 17 24 19 32 32 37 37 31 30 30 28 31 30
+34 34 28 28 28 28 31 31 30 28 28 28 30 30 25 32 10 10 10 23 23 27 17
+17 22 17 23 23 23 24 20 17 4 4 4 14 16 24 22 19 18 13 19 9 12 14 25 25
+33 32 29 40 38 49 49 49 34 34 17 14 8 7 7 9 9 10 8 7 7 9 10 15 17 18
+22 24 18 18 18 22 20 24 24 28 18 19 18 19 21 24 25 14 12 8 8 9 14 12 4
+4 4 17 17 17 14 9 9 9 12 21 21 18 12 10 13 10 11 9 12 12 8 7 8 8 9 10
+9 15 10 8 9 10 10 21 21 28 27 18 14 7 7
+
+
+Sequence : 22ak93c1.s1lt
+Is_read
+Padded
+Staden_id 1
+Clipping QUAL 56 598
+Align_to_SCF 1 648 1 648
+SCF_File 22ak93c1.s1ltSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_primer
+Template 22ak93c1
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 47 "M13mp18"
+Tag DONE 541 541 "AUTO-EDIT: replaced N by g at 541 (all_stranded, isolated, strong)"
+Tag ALUS 559 647
+Tag DONE 569 569 "AUTO-EDIT: replaced A by g at 569 (all_stranded, isolated, strong)"
+Tag DONE 571 571 "AUTO-EDIT: replaced A by g at 571 (all_stranded, isolated, strong)"
+
+DNA : 22ak64e2.s1t
+GTAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTGCAAAATGCTTGCTTA
+GGACCAGAAATGTTTCAGATTTCAGATTTTTTTTGGAATATTTGCATTTTATAGTACTTA
+CTGGTTGAATATTCCCAACCCAAAAATCTAACAACCCATAATGCTCCAATAAGCACTTCC
+TTTGAACATTAGGTCGGTGCTCAAAAAAGTTTCAGACTGTAGAGCATTTCTAATTTTGGA
+TTTTCAGATTAGAAACACTCAACCCGTATAATAAAATGATAGTTTGTATTAAGCACAGCA
+CTATATCAATCACCTCACAGCTATTCTCTCATTTAATCATCATAATAACCCTGTAAGTAC
+TGTACTAGTTGTCTTGTCTCTGCTTTTTTTTTTTCGAGACAGAGTCTCACACTATTGGTT
+GGACTGGAGTGCAATTCnCCTGAATTCGGCTCACGGCAACTTCAACCTCCCGGGTTCAAG
+TGAATCTCCTC
+
+BaseQuality : 22ak64e2.s1t
+4 4 8 10 12 15 18 18 28 30 30 30 30 26 37 34 30 30 22 22 20 23 16 11
+11 18 23 23 23 28 9 9 10 9 21 12 32 34 45 45 45 45 38 30 30 30 30 30
+28 34 30 33 33 33 33 33 43 45 45 32 18 10 11 11 25 23 10 10 22 32 38
+37 27 27 27 23 20 9 9 10 23 25 25 21 9 9 10 29 32 45 45 45 45 45 45 37
+37 37 38 45 37 41 28 29 28 28 28 28 34 34 33 33 33 28 28 34 33 34 34
+34 34 34 34 45 37 37 29 37 37 45 45 45 45 42 37 34 35 35 35 34 34 34
+34 34 37 37 37 45 45 35 35 35 35 35 35 35 37 37 37 37 45 37 42 42 33
+30 30 33 33 33 40 34 34 34 34 34 34 40 40 40 37 37 37 34 34 34 34 34
+31 31 27 28 19 21 19 28 28 30 32 34 34 31 31 31 33 39 27 20 7 7 8 8 14
+15 11 11 17 28 23 28 17 9 9 11 23 27 33 33 40 40 40 40 40 40 40 37 32
+32 31 31 28 37 30 33 25 21 13 13 14 26 22 11 11 21 21 30 33 31 31 33
+37 37 37 45 49 45 40 40 40 40 40 45 40 40 40 40 40 31 29 29 32 27 37
+37 45 45 45 40 37 37 37 37 37 33 34 33 33 30 30 34 34 36 36 36 30 30
+28 28 28 27 38 38 36 31 33 32 27 20 20 20 28 32 33 24 24 29 36 34 30
+33 30 26 26 26 30 30 26 37 36 34 33 30 31 31 28 27 27 25 24 29 33 27
+27 31 31 31 31 33 36 38 38 32 32 21 13 13 16 19 19 24 25 33 30 33 33
+33 38 39 40 44 40 40 36 33 37 40 40 40 40 49 40 36 33 31 26 23 23 21
+18 20 14 12 11 14 17 25 31 33 33 33 36 30 30 30 30 30 28 28 20 15 14 4
+4 4 12 9 9 4 4 4 7 8 7 7 7 7 4 4 4 7 8 10 7 6 9 9 9 7 7 11 17 19 9 8 7
+7 8 7 8 9 7 6 10 6 7 6 6 7 7 8 8 8 12 13 8 8 8 8 10 10 9 8 7 6 6 8 13
+7 7 7 7
+
+
+Sequence : 22ak64e2.s1t
+Is_read
+Padded
+Staden_id 14
+Clipping QUAL 41 420
+Align_to_SCF 1 491 1 491
+SCF_File 22ak64e2.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak64e2
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 40 "M13mp"
+
+DNA : 22ak70f10.s1t
+TTGGCTGCCTGCAGGTCGACTCTAGAGGATCCCCCAGGGTTATTATGATGATTAAATGAG
+AGAATAGCTGTGAGGTGATTGATATAGTGCTGTGCTTAATACAAACTATCATTTTATTAT
+ACGGGTTGAGTGTTTCTAATCTGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACT
+TTTTTGAGCACCGACCTAATGTTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAG
+ATTTTTGGGTTGGGAATATTCAACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAA
+ATCTGAAATCTGAAACATTTCTGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCT
+GTAGTACGTTCTTTATCATTGTTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGT
+GGTATAGCAAATTCGATTGTATTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAG
+GATTATTGGAGAGAATAAAACAGTGAAGCTTTGGTGTTATGAGGGAATnTTAGATAGAAA
+AGTGCAGTnTTTCAGTTCATGCTCTnTCATTTnTTACTCCCTCCGGTTAAAGCTGAAGCT
+CAACAAAGATATAGTGATCTCTGTGGGCATTATAATCCTGTCCC
+
+BaseQuality : 22ak70f10.s1t
+4 4 6 8 8 9 19 23 33 33 34 33 27 21 18 27 18 27 27 34 34 31 27 23 13
+11 19 16 31 31 37 45 45 34 34 31 31 22 31 25 31 37 45 45 45 45 37 37
+37 37 37 37 45 37 37 37 45 38 29 27 27 32 32 37 45 33 37 37 45 37 45
+45 37 37 37 40 40 40 37 45 37 37 34 33 33 34 34 34 37 33 40 37 37 37
+33 33 33 33 33 33 33 33 33 32 33 41 41 35 35 35 40 40 36 36 36 34 33
+33 37 37 37 37 37 41 45 30 30 41 41 37 28 45 18 18 29 35 37 37 37 37
+41 41 51 51 51 51 51 33 33 33 33 33 33 45 45 45 41 39 39 51 51 41 45
+45 45 45 35 33 33 33 33 33 40 51 51 51 51 51 51 51 51 51 43 45 39 34
+30 30 33 33 33 33 45 45 38 38 38 38 33 30 29 14 15 27 27 30 33 51 51
+41 41 37 34 37 37 37 40 40 40 37 45 51 33 33 33 33 33 33 37 37 31 45
+33 25 22 27 21 28 33 28 28 33 41 37 45 41 33 45 33 22 22 33 33 34 44
+44 45 45 33 33 33 33 33 30 34 34 34 34 37 37 29 34 37 33 33 33 33 33
+33 34 40 33 33 33 30 30 30 37 37 37 30 30 33 30 33 33 49 49 45 45 45
+45 45 45 44 38 38 38 44 38 45 45 49 40 40 35 35 35 35 38 37 40 37 37
+34 34 34 34 34 34 34 36 36 36 34 36 33 31 31 36 33 28 28 27 23 19 17
+17 23 23 23 33 27 28 28 28 30 33 40 40 31 31 17 20 20 28 32 25 17 17
+32 27 37 37 36 33 33 33 33 32 36 34 28 30 32 31 31 14 18 10 9 9 19 21
+30 31 38 38 38 37 37 31 34 34 36 31 30 31 31 31 31 33 33 33 39 30 25
+24 24 29 33 33 34 33 33 23 23 12 23 23 30 33 33 37 37 37 38 40 37 34
+33 37 36 33 33 33 33 33 33 36 28 28 22 24 24 24 21 29 33 33 33 34 33
+33 33 33 33 33 33 33 33 27 28 28 31 31 23 27 19 15 11 20 21 33 33 31
+31 31 31 31 26 28 24 24 24 29 24 29 29 33 27 31 27 30 36 29 18 20 12
+17 9 9 9 19 19 15 8 8 13 15 14 8 9 9 9 10 12 4 4 4 9 10 11 12 24 26 18
+13 13 11 11 13 10 16 14 13 12 4 4 4 17 11 7 7 8 7 8 8 8 20 20 26 16 14
+4 4 4 8 8 10 18 4 4 4 8 8 21 11 13 10 8 10 6 6 6 6 6 8 9 12 14 17 11
+14 14 8 8 8 7 9 7 8 8 12 9 9 8 8 7 10 10 12 10 9 8 16 9 8 8 8 7 6 6 8
+6 6 6 6 6 6 6 9 12 7 7 8 8 7 7 7 12 8 7 7
+
+
+Sequence : 22ak70f10.s1t
+Is_read
+Padded
+Staden_id 2
+Clipping QUAL 35 508
+Align_to_SCF 1 644 1 644
+SCF_File 22ak70f10.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak70f10
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 34 "M13mp1"
+Seq_vec SVEC 584 644 "M13mp1"
+
+DNA : 22ak92c4.s1t
+nGGGCGCTGCCTGCAGGTCGACTCTAnAGGATCCCCATTATACGGGTTGAGTGTTTCTAA
+TCTGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAA
+TGTTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGGGTTGGGAATAT
+TCAACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAATCTGAAACATT
+TCTGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCTGTAGTACGTTCTTTATCAT
+TGTTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAGCAAATTCGATTG
+TATTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAGGATTATTGGAGAGAATAAA
+ACAGTGAAGCTTTGGTGTTATGAGGGAATTTTAGATAGAAAAGTGCAGTTTTTCAGTTCA
+TGCTCTTTCATTTC
+
+BaseQuality : 22ak92c4.s1t
+4 4 6 4 6 8 11 14 18 26 32 34 36 34 23 25 10 23 9 23 23 34 29 23 22 4
+4 4 16 14 25 24 29 33 30 33 33 33 33 40 37 40 45 28 28 25 15 18 32 32
+17 14 28 13 13 38 38 40 40 37 37 37 37 38 45 45 45 38 33 32 33 33 33
+33 38 38 38 45 32 30 45 32 34 34 34 34 40 33 33 32 32 28 28 38 45 45
+49 45 45 45 45 40 37 41 45 45 40 30 30 33 33 33 33 40 40 45 45 45 45
+32 32 28 11 12 27 25 33 33 45 41 33 32 14 14 28 33 37 37 37 37 37 45
+45 37 37 37 37 37 37 37 37 28 33 32 21 14 32 20 24 32 21 21 32 36 41
+41 45 32 32 28 13 13 33 33 45 51 51 41 37 32 32 32 32 32 32 34 34 30
+30 31 32 12 12 27 31 30 35 35 35 33 39 41 28 28 28 28 28 28 41 41 41
+30 32 30 30 30 32 51 51 51 51 45 45 45 45 45 45 45 45 51 45 45 45 45
+33 33 33 33 33 33 51 51 51 51 51 41 41 45 45 45 39 39 39 39 39 39 41
+41 41 41 40 33 33 33 33 33 33 41 45 41 41 41 41 33 33 33 33 33 33 51
+51 45 34 22 22 20 28 30 17 15 15 45 30 33 33 33 33 33 33 33 33 37 37
+32 33 33 25 25 9 10 9 14 14 33 33 34 34 41 40 40 40 40 37 40 37 37 34
+33 33 29 24 22 32 32 45 45 40 27 27 14 32 32 45 38 34 45 19 21 10 21
+21 30 37 37 34 34 37 38 38 37 37 37 37 37 35 34 36 36 40 40 49 34 34
+34 34 37 37 38 37 33 30 30 30 30 30 40 40 34 34 34 27 27 31 31 33 35
+33 30 30 21 21 12 30 28 36 40 36 36 36 33 33 26 26 21 19 20 29 30 36
+34 34 33 31 28 28 32 35 32 35 26 30 25 26 26 25 21 19 12 11 19 21 23
+21 11 13 17 17 24 29 27 29 29 28 31 20 25 18 16 12 14 23 21 11 11 16
+14 10 21 23 33 33 34 31 31 22 22 14 21 11 16 18 31 40 40 36 36 24 27
+19 19 13 16 10 8
+
+Sequence : 22ak92c4.s1t
+Is_read
+Padded
+Staden_id 16
+Clipping QUAL 37 490
+Align_to_SCF 1 494 1 494
+SCF_File 22ak92c4.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak92c4
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 36 "M13mp1"
+
+DNA : 22ap08f1.s1t
+GAACTATCATnnnATATACGGGTTGAGTGTnTCTAATCTGAAAATCCAAAATTAGAAATG
+CTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAATGTTCAAAGGAAGTGCTTATTGGA
+GCATTATGGGTTGTTAgATTTTTGGGTtGGGAATATTCAACCATTAAGCTACACACAATG
+CAAATATCCCACAAACAATCTGAAATCTGAAACACTTCTGGTCCAAGCAAGCATCCGCAA
+CGGATACGCAACCTGCACACGTTCTTATCATCGTTAACTCTnATATATGTGGACAAATCT
+GACGTGGTACACACACTCATGTATATCACAACATATTACATTTGAAGCTGCTACGAATAT
+GGAAGAATnnnnCCTGAACTTGGTGTATGACGAACTTAGATAGACAGTGACTCTTCAGTT
+CAGCTCnTCATTTnTnnTCCCTCGGTTTTnCTAAnCnCnnCnnAAAAnnnnATCCnGTGG
+GCATAAAnnCnGGTCCCACCGCCCCCnCCCCACAAAACCACACACTACCGACCTCCCAA
+
+BaseQuality : 22ap08f1.s1t
+7 9 10 13 14 16 21 15 14 4 4 4 4 4 11 11 20 26 28 28 30 30 30 25 25 20
+25 23 28 4 4 4 18 18 18 21 24 31 33 34 33 34 34 40 30 31 30 24 24 24
+31 27 30 31 30 23 32 32 37 36 38 34 33 24 18 15 10 8 8 18 25 32 33 34
+31 31 33 29 21 12 9 7 8 11 13 18 22 25 25 33 33 32 29 22 27 24 25 16 9
+9 19 21 20 14 16 10 12 12 11 11 18 23 21 19 19 10 10 10 20 9 9 13 18
+18 20 21 18 17 14 16 9 9 9 10 10 10 9 9 21 25 22 18 18 14 20 21 13 13
+21 17 26 25 23 20 15 10 12 16 17 15 10 11 8 8 8 8 6 4 4 4 8 10 8 8 6 6
+8 13 15 21 21 15 11 9 12 11 7 6 6 6 6 6 6 10 6 6 6 6 10 14 10 10 9 12
+9 11 10 14 9 13 10 10 12 9 9 15 17 20 16 17 21 25 10 8 10 10 7 7 10 9
+7 8 14 7 7 8 10 8 6 6 6 8 8 10 11 14 8 9 11 19 17 13 9 9 9 8 8 10 9 8
+8 8 9 8 11 11 10 8 8 8 8 6 6 8 10 8 7 6 4 8 4 4 4 7 7 11 10 12 9 12 10
+10 10 11 6 6 9 9 14 8 7 7 7 7 12 16 11 6 6 6 6 6 6 6 6 6 8 13 9 8 8 15
+12 10 9 8 8 8 8 7 9 12 8 6 6 8 4 6 6 7 11 13 13 7 8 9 7 7 12 12 10 9 4
+4 4 6 7 7 12 14 7 7 12 6 6 6 7 4 4 4 4 4 4 8 8 14 12 13 16 16 16 10 11
+16 14 12 7 6 6 4 4 4 8 7 7 7 12 7 7 8 11 4 4 4 4 4 6 6 6 6 6 6 6 6 6 4
+4 8 6 6 12 12 12 9 8 4 4 4 6 11 6 9 4 4 4 4 4 4 9 9 9 15 9 9 6 6 6 8 4
+4 4 9 9 4 4 4 4 4 4 4 4 4 4 4 9 9 4 4 4 4 4 4 11 12 4 4 4 8 8 15 9 6 8
+9 9 9 4 4 4 4 4 4 7 10 6 8 15 8 8 4 6 14 15 17 18 4 4 4 14 8 6 6 8 8 9
+13 9 9 9 7 7 7 7 8 8 6 6 8 6 6 6 8 6 6 8 8 8 8 4
+
+Sequence : 22ap08f1.s1t
+Is_read
+Padded
+Staden_id 17
+Clipping QUAL 17 155
+Align_to_SCF 1 539 1 539
+SCF_File 22ap08f1.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ap08f1
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Tag DONE 137 137 "AUTO-EDIT: replaced T by g at 137 (terminated, isolated, strong)"
+Tag DONE 148 148 "AUTO-EDIT: replaced A by t at 148 (terminated, isolated, strong)"
+
+DNA : 22ai01c4.s1t
+GGAACATGCTGCAGGTCGACTCTAGAGGATCCCCGCTCTACAGTCTGAAACTTTTTTGAG
+CACCGACCTAATGTTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGG
+GTTGGGAATATTCAACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAA
+TCTGAAACATTTCTGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCTGTAGTACG
+TTCTTTATCATTGTTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAGC
+AAATTCGATTGTATTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAGGATTATTG
+GAGAGAATAAAACAGTGAAGCTTTGGTGTTATGAGGGAATTTTAGATAGAAAAGTGCAGT
+TTTTCAGTTCATGCTCTTTCATTTTTTACTCCCTCAGGTTAAAGCT-GAAGCTCAACAAA
+GATATAGTGATCTCTGTGGGCATTTATAATCTGGTCCAGAAGGCTCTCAAGCCTCCTCCA
+ATAAAGCTCTATCGGGAAACAAATGAAACAGTGAAAACCAAGAACCGGAACTTTAATACA
+AGTACAGGCGGTTTGCTTTCTGCTAGCGATACCAAAAGTCTCAGGTAGGTAAAAATGCTn
+nTGTGTTA
+
+BaseQuality : 22ai01c4.s1t
+4 4 6 8 8 18 18 25 25 30 25 21 27 16 16 27 24 27 31 37 33 33 31 31 21
+21 23 25 37 34 37 45 45 45 45 40 40 40 33 33 30 30 30 30 38 45 45 49
+45 45 45 45 45 45 45 45 38 38 30 30 30 33 33 30 37 37 40 42 42 45 38
+45 35 25 27 33 33 33 33 37 37 37 40 37 37 37 37 42 42 42 40 37 40 45
+33 33 33 33 33 33 45 45 41 41 45 37 30 41 32 41 45 37 37 41 41 45 45
+41 32 38 37 30 30 30 41 41 51 51 51 51 33 33 35 35 35 29 33 33 33 33
+33 45 37 37 37 37 37 40 40 40 40 45 43 35 33 33 33 33 33 43 45 43 32
+33 33 28 28 29 51 51 45 51 51 51 51 51 51 51 51 51 51 51 51 51 51 42
+42 42 40 39 39 51 51 45 45 45 38 38 37 37 38 33 33 33 33 33 33 37 40
+39 39 42 33 33 33 33 33 33 37 37 41 41 41 41 33 33 33 33 33 33 51 45
+41 41 39 34 34 29 31 37 34 34 45 37 38 42 33 32 32 32 32 32 37 51 32
+32 32 32 32 21 24 12 25 25 33 33 33 33 40 40 40 37 37 41 45 45 45 35
+34 34 34 34 34 41 41 45 45 45 41 37 37 41 40 45 37 37 42 33 33 22 33
+33 33 37 37 37 37 37 37 37 37 37 36 33 33 33 33 33 33 40 40 45 34 34
+34 34 34 34 37 37 40 33 33 33 33 33 36 36 40 34 37 37 37 37 38 38 36
+33 33 33 31 31 20 29 27 34 34 34 40 40 34 34 38 38 38 34 34 40 37 40
+37 30 30 30 30 30 34 36 40 40 36 36 28 33 32 27 27 18 14 14 18 23 27
+34 33 29 29 24 29 29 27 27 28 28 25 25 33 22 24 23 31 28 33 34 31 25
+25 25 25 30 31 36 32 27 18 13 13 25 25 17 23 25 31 45 45 45 38 31 31
+24 22 20 27 35 34 31 33 33 33 33 36 36 36 38 20 13 10 8 12 11 18 20 23
+34 34 30 1 29 22 19 24 22 24 28 31 31 31 27 27 23 28 10 25 20 17 10 25
+25 35 35 38 38 36 36 27 27 17 17 11 13 16 19 18 18 18 22 15 11 11 15
+27 25 24 24 18 8 9 8 7 7 8 7 8 8 15 17 21 14 10 10 19 17 20 25 24 24
+21 16 15 21 21 26 33 33 26 23 19 23 23 28 21 20 15 10 8 7 8 6 8 11 7
+10 18 17 12 9 9 7 11 9 7 6 7 6 8 9 9 9 6 10 8 10 7 8 8 7 6 6 8 7 8 8
+14 23 24 19 14 14 7 7 7 7 8 8 6 6 7 6 6 6 6 6 9 21 13 12 13 15 15 10
+11 17 16 15 8 7 7 9 9 10 10 8 8 8 8 9 8 6 8 8 8 7 7 7 7 7 7 7 7 7 7 7
+10 8 7 7 8 11 4 4 4 4 9 6 6 6 6 6
+
+
+Sequence : 22ai01c4.s1t
+Is_read
+Padded
+Staden_id 6
+Clipping QUAL 35 553
+Align_to_SCF 1 466 1 466
+Align_to_SCF 468 668 467 667
+SCF_File 22ai01c4.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ai01c4
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 34 "M13mp18"
+
+DNA : 22ak93c1.s1t
+nGGTGAAGCTTGCATGCCTGCAGGTCGACTCTAAAGGATCCCCACAcCAAAGCTTCACTG
+TTTTATTCTCTCCAATAATCCTAAgCAAGCTCTCAAAATATAAATATGCTTTtTAATAAT
+ACAATCGAATTTGCTATACCACCTCAGAATCTGTACCACAATATATTAACTACTTAAAAC
+AATGATAAAGAACGTACTACAGGTTGCGTATCCCTTTGCAAAATGCTTGCTTAgGACCAG
+AAATGTtTCAGATTTCAGATTTTTTTTGGAATATTTGCATTTTATAGTACTTACTGGTTG
+AATATTCCCAACCCAAAaATCTAACAACCCATAATGCTCCAATAAGCACTTCCTTTGAAC
+ATTATGTCGGTGCTCAAAAAAGTTTCAGACTGTAAAGCATTTCCTAATTTTGGATTTCCA
+GAATAGAAACACTCAACCCGTATAATAAAATGATAATCTGTATTAAGCACAGCACTATAT
+CAAGCACCTCACAGCTATTCTCTCATTTAATCATCATAATAACCCTGTAAGTACTGTACA
+AGTGGTCTGGTCCCTGCTTTTATTTCTCCAAGAACGACTCTCACACTATTGGTTCGCCTG
+GAGTGCnTCGCGTGATCCCCGCTCATGGCACCTCCACTCCCGGGTCCAAGCAATTCTCTT
+GCTCAACTTCCAAGTGGTGGGATTAGGGCCCTGCCATAACCCACTTATTTCTTTTATTTT
+AATAAAACGTTTCCCCATTTTGGCAGTGGGTTTTACCCTGACCCGTATTGCCCTTCCGGC
+CCACGATTTGGGGGAACCAGTTGGGCCCCCCTTTGGGCCTCTCCTGTTCCGAGC
+
+BaseQuality : 22ak93c1.s1t
+4 4 4 4 8 8 12 20 25 31 33 33 30 30 30 31 31 35 35 34 27 23 12 11 25
+11 14 14 28 32 20 14 6 6 6 6 8 16 16 25 28 15 15 13 17 9 10 9 16 16 18
+18 18 25 27 30 26 20 21 19 10 10 14 23 25 30 30 37 37 37 34 31 28 30
+22 18 29 18 22 18 15 11 21 12 15 15 22 24 28 37 37 37 37 32 27 10 10
+13 18 20 25 25 23 23 24 32 33 40 40 38 32 18 18 18 27 27 28 37 37 21
+21 15 15 12 22 32 23 23 30 30 45 45 37 37 32 32 23 22 22 22 27 28 31
+26 23 12 12 18 32 38 37 37 37 37 34 34 30 30 30 30 30 30 34 34 37 37
+37 34 34 31 31 31 28 27 24 22 22 22 30 30 33 30 30 32 26 24 15 15 9 7
+7 14 10 10 9 14 11 12 10 11 6 7 9 8 17 24 26 28 26 28 28 35 34 34 30
+26 26 26 28 28 31 31 33 33 31 23 20 15 13 15 18 14 6 6 8 13 13 19 9 7
+7 16 10 10 15 10 8 8 12 10 9 9 18 13 14 17 11 7 7 13 21 23 28 21 20 19
+19 22 25 27 31 32 32 28 28 25 25 25 25 25 25 31 30 19 19 13 13 13 27
+28 33 33 33 33 33 36 33 31 24 21 14 15 18 24 27 31 25 28 30 30 28 23
+23 21 17 14 8 9 9 25 25 33 25 25 28 30 30 30 22 21 20 20 22 25 28 34
+28 21 16 15 10 14 17 25 25 12 12 12 12 12 20 10 14 16 21 17 13 11 9 12
+11 7 6 6 6 8 14 8 8 6 6 11 23 21 28 28 31 31 34 23 10 7 7 7 7 7 9 8 8
+14 16 20 20 16 10 7 7 7 14 7 9 14 10 9 14 9 19 22 27 25 13 13 18 14 17
+23 16 9 8 9 7 7 7 7 9 8 8 13 16 21 19 15 15 10 10 15 11 18 25 23 21 21
+21 32 25 28 25 28 17 20 10 7 7 11 6 7 6 6 7 9 8 6 6 6 6 6 8 7 7 9 13
+11 13 13 20 21 18 15 10 10 9 9 8 8 8 9 9 13 16 16 14 14 14 25 10 11 6
+6 6 6 7 10 14 9 13 20 8 7 7 8 11 10 13 12 12 13 16 14 15 15 13 13 10
+12 7 8 9 14 11 12 12 9 10 8 8 8 10 20 12 9 9 12 6 6 6 6 8 9 6 6 6 6 7
+6 6 8 16 18 18 9 6 6 6 13 7 7 7 8 9 9 7 7 7 7 7 6 6 6 6 6 6 6 6 7 8 8
+10 9 8 8 6 7 8 4 4 4 4 4 10 7 7 9 9 8 8 4 4 4 4 4 4 8 4 9 9 12 9 8 6 6
+6 9 11 7 7 7 7 7 9 11 11 9 6 6 6 10 8 8 7 10 10 14 25 16 10 7 7 7 7 10
+10 8 8 7 7 7 8 6 6 6 6 8 11 12 7 6 13 6 7 7 7 8 7 7 7 8 8 10 8 6 6 6 4
+4 8 8 10 13 22 24 24 20 14 8 6 6 6 6 6 10 15 15 10 9 6 6 10 8 7 7 9 9
+13 13 10 11 11 11 8 7 7 6 14 6 7 8 6 6 7 7 7 7 7 7 12 7 8 10 10 13 14
+9 8 8 8 11 9 7 7 7 8 8 11 10 12 11 11 10 10 12 12 17 13 9 8 9 6 6 6 6
+6 12 8 8 8 8 9 8 8 9 8 7 7 7 9 9 8 8 6 6 8 8 11 12 8 6 8 6 6 6 6 6 7 8
+9 11 12 12 6 6 8 7 8 8 13 7 7 7 6 8 8 8 8 8 8 6 6 8 4 6 4 4 4 4
+
+Sequence : 22ak93c1.s1t
+Is_read
+Padded
+Staden_id 10
+Clipping QUAL 44 345
+Align_to_SCF 1 834 1 834
+SCF_File 22ak93c1.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak93c1
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 43 "M13mp1"
+Tag DONE 47 47 "AUTO-EDIT: replaced T by c at 47 (terminator, isolated, strong)"
+Tag DONE 85 85 "AUTO-EDIT: replaced C by g at 85 (terminated, isolated, strong)"
+Tag DONE 113 113 "AUTO-EDIT: replaced A by t at 113 (terminated, isolated, strong)"
+Tag DONE 234 234 "AUTO-EDIT: replaced C by g at 234 (terminated, isolated, strong)"
+Tag DONE 247 247 "AUTO-EDIT: replaced C by t at 247 (terminated, isolated, strong)"
+Tag DONE 318 318 "AUTO-EDIT: replaced G by a at 318 (terminated, isolated, strong)"
+
+DNA : 22ak65e10.s1t
+TGAGGGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAAAATAAATAAATAA
+ATAAATTAGCTGGGTGTGATGTCACATGCCTGTGATCCCAGCTACTTGGGAGGCTGAGAC
+AGAAGGATCGCTTGAGTCCAGGAGGTCAAGGCTGTAGCGAGTCATGTTCGCCCCACTGCA
+CTCCAGCCTGGGCGACACAATGAGACCCTGTCTCAAAAACAACAACAACAAAAGGCATCT
+CTACCTACCTGAGACCTCTTGGTATCGCTAGGCAGAAGCAAACCGCCTGTACTTGTATTA
+AAGGTCCGGGTCTTGGTTTTCACTGGTTCATTTGTTTCCCGATAGAGCTTTATTGGAGGA
+gGCTTGAGAGCCTTCTGGACCAGATTATaAATGCCCACAGAGATCACTATATCTTTGTTG
+AGCTTC-AGCTTTAACCTGAGGGAGTAAAAAATGAAAGAGCATGAACTGAAAAACTGCAC
+TTTTCTATCTAAAATTCCCTCATAACACCAAAGCTTCACTGTTTTATTCTCTCCAATAAT
+CCTAAGCAAGCTCTCAAAATATAAATATGCTTTTTAATAATACAATCC
+
+BaseQuality : 22ak65e10.s1t
+4 4 8 8 9 9 14 15 31 31 28 28 28 30 30 33 33 33 30 22 20 12 22 12 18
+18 30 31 27 23 32 10 10 14 12 32 32 37 45 45 45 45 45 38 34 29 29 29
+29 29 29 29 32 32 30 34 34 34 34 35 34 34 32 35 29 27 32 32 32 37 45
+32 45 37 37 37 24 24 32 32 19 22 11 21 21 31 31 34 45 45 45 28 30 30
+37 40 34 33 33 28 28 28 37 45 41 41 41 41 45 41 27 27 33 37 37 32 27
+20 20 20 30 20 20 21 24 17 38 32 30 42 37 37 37 30 28 13 12 18 25 21
+24 14 25 25 22 28 17 28 28 18 32 36 36 32 32 21 32 32 31 31 27 27 21
+32 32 27 13 13 32 33 38 45 36 34 30 30 21 21 23 23 23 23 30 33 33 37
+34 41 36 41 37 37 34 29 29 19 30 30 40 37 37 36 37 25 25 26 26 30 45
+41 51 37 33 30 28 28 33 20 18 20 20 20 20 26 20 20 18 21 21 31 31 28
+32 14 18 20 29 33 34 45 45 40 40 40 37 37 37 37 37 32 45 30 23 23 32
+32 37 34 35 34 34 34 34 37 37 37 37 45 37 37 32 27 23 14 12 12 14 17
+19 27 27 28 30 37 45 45 45 45 37 37 37 42 42 40 37 37 34 33 33 40 31
+26 28 25 27 31 34 34 34 31 27 27 27 31 27 31 23 23 18 18 18 25 25 31
+35 31 30 27 25 27 35 33 32 30 21 21 21 23 23 27 26 21 20 16 16 17 18
+30 30 36 36 36 34 34 36 34 26 15 12 6 6 6 12 11 23 23 26 17 17 21 32
+35 35 40 33 33 33 38 33 27 23 23 12 12 18 18 19 10 8 8 17 19 34 33 34
+31 34 31 31 22 22 19 18 27 27 32 22 25 24 32 32 34 34 34 37 34 30 33
+27 27 27 31 32 27 27 18 1 12 12 11 15 15 17 27 28 28 34 34 30 25 18 13
+19 15 15 25 21 29 26 34 24 28 25 27 21 20 23 24 24 26 29 30 26 27 22
+29 27 33 34 34 36 38 38 38 34 31 30 24 24 24 31 33 35 35 29 33 33 31
+31 28 28 29 26 22 14 8 9 9 9 14 9 13 18 21 21 27 23 18 15 21 21 25 33
+33 31 31 21 21 24 27 29 25 19 19 19 25 27 27 34 34 34 14 9 7 7 9 18 23
+27 23 19 19 23 24 21 18 8 8 9 21 20 31 24 20 13 11 16 24 32 32 25 18
+13 13 20 20 26 21 27 21 20 13 17 15 21 12 12 18 15 15 15 15 8 8 7 10 8
+4
+
+
+Sequence : 22ak65e10.s1t
+Is_read
+Padded
+Staden_id 4
+Clipping QUAL 42 575
+Align_to_SCF 1 426 1 426
+Align_to_SCF 428 588 427 587
+SCF_File 22ak65e10.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak65e10
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 41 "M13mp"
+Tag DONE 361 361 "AUTO-EDIT: replaced C by g at 361 (all_stranded, compound, strong)"
+Tag DONE 389 389 "AUTO-EDIT: replaced C by a at 389 (terminated, isolated, strong)"
+
+DNA : 22ak93c2.s1t
+CTGGGAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCACTCATTTTCTGCCTGG
+TGTTTATTCTCATCCAAATTCGTATACATGCTCGGTGTGAATGGTTACTCATTATAGTAT
+ACAATCGACTTTCTCAAACGACCACACATGCTGAATATCTATACATGTGCTAGTTGGACC
+TCTGATTATCAACGTGAGACTGGTGGCGTATCCCTTTGCAAAATGCTAACAAAAGACCAC
+AAACGTGTCACATTTCAGAATTGCTTGGAATATTCGCGTGTATAGTACTTACTGGTTCAA
+GGGTCCAAAACATACCTCTACACACCATCCTGATTCCCTGGGACACTTCCTTTGAACATC
+ACGTCGGTGCTCAAAAAAGTGTCAGACTGTAGAGCATATCTGGTTTCGGAATGTCGAGAT
+AGAAGGTCTCAAGCCGTATAAATAAACGAGAGTCTGTATCAAGCACTGCAGGAGAATCAT
+AACCTCACAGCTATGCTCTCATGGAAACATCTCAATACACCTCGTAGTACTGTACTAATA
+GTCATGTCGCTGCTTTTTGTTnCCGACAACCAATCTCACGGATTGGTTCGGCCGGTTTGC
+ATCCCTGATTCCGATCATTGAAGGTCGAACTCCCGGTCCAAGCAATCTCCTGCCCCGGCT
+CCAGATGACGGGATTCTTGCCTTTCAACACCCCGGCTAATTCATCCTCTCCCTCGACACC
+GATTTCACGAAATGGGGGTCGGCCCCAACCCGAACATTTGTTTACTGGGTAAAACGGTTC
+CCCAGTTACCCGTTGGCCCACTCCTGGGCCATTAATTGCCTC
+
+BaseQuality : 22ak93c2.s1t
+4 4 8 8 9 12 12 22 27 30 30 30 30 33 33 35 33 37 37 33 31 30 25 25 27
+27 18 18 32 38 32 28 28 9 9 10 10 25 21 27 38 28 25 23 16 12 10 10 11
+10 13 12 11 15 14 8 8 11 13 18 21 21 9 7 7 6 11 8 8 8 6 6 8 8 8 8 10 9
+9 10 9 14 9 13 11 8 8 8 8 8 11 10 11 14 14 14 21 11 10 10 10 11 17 11
+7 7 8 8 9 9 13 14 18 15 9 9 9 8 8 10 12 12 10 10 8 9 12 12 13 14 23 10
+10 10 12 11 11 10 10 10 11 14 14 15 15 21 22 10 10 10 10 28 10 9 9 9
+10 12 15 18 15 15 12 12 12 12 25 21 24 27 18 13 13 13 14 12 14 12 10
+10 10 14 18 14 9 9 11 12 12 10 10 10 10 9 14 14 8 8 8 8 6 6 9 6 6 6 12
+18 34 36 38 38 38 38 36 36 33 33 23 23 13 13 15 23 28 20 9 6 6 4 6 10
+8 7 7 9 10 9 13 13 10 10 9 8 8 6 6 8 8 11 12 10 9 9 9 6 4 6 6 8 6 7 11
+10 9 7 8 17 18 21 19 17 11 8 8 9 11 11 15 12 8 8 8 9 9 15 15 10 10 9
+10 7 7 8 12 10 10 9 9 9 9 10 11 10 8 8 8 7 12 7 7 7 8 9 8 9 10 10 9 10
+8 6 6 6 6 7 6 9 10 10 10 7 6 6 6 8 6 6 9 10 13 12 12 9 12 15 14 14 8 8
+8 9 10 10 9 9 9 9 8 6 6 10 8 13 6 6 6 11 16 18 12 11 8 10 12 8 7 9 7 7
+7 7 9 9 7 7 10 10 12 10 20 9 7 7 7 8 8 8 9 7 7 7 11 9 13 19 15 12 7 8
+7 6 6 6 6 6 6 6 10 13 8 6 6 8 6 8 10 10 12 7 7 7 7 7 7 8 10 9 9 8 7 6
+6 8 6 6 8 9 9 10 7 7 8 8 8 8 7 7 8 7 7 7 7 7 9 8 8 15 8 8 6 6 6 6 6 6
+7 10 9 9 8 7 8 8 10 7 7 8 8 11 11 11 8 6 6 6 6 6 12 6 6 6 6 6 7 11 12
+9 7 10 11 8 8 7 8 11 8 7 7 7 6 8 6 6 8 6 8 7 7 8 7 7 7 8 9 7 7 7 7 7 7
+9 7 7 7 6 13 4 7 7 7 12 23 21 14 12 9 8 9 8 4 4 4 6 6 6 7 6 8 4 4 4 6
+6 6 6 6 6 8 6 7 10 9 9 9 8 6 4 4 4 6 8 6 6 6 6 8 6 6 8 8 8 8 8 8 6 6 8
+11 10 7 7 7 7 9 9 9 8 7 11 8 7 7 8 9 8 8 7 7 7 7 7 7 7 7 8 7 7 7 7 8 7
+7 8 7 7 7 7 8 7 7 7 7 7 7 6 6 7 7 6 6 6 8 9 8 7 7 7 7 8 8 12 9 7 7 7 7
+6 7 8 7 7 7 8 8 6 6 6 6 6 6 7 7 9 12 10 14 8 9 8 6 6 6 6 6 6 9 10 10 9
+6 6 6 6 8 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 7 7 7 6 6 6 4 6 6 6 6 8 6
+6 6 6 6 8 8 10 7 4 7 4 4 4 6 6 6 6 6 6 6 6 6 6 6 7 7 9 9 8 8 7 9 9 7 7
+7 8 6 6 6 6 6 6 6 6 6 6 8 8 8 8 8 8 8 7 7 6 6 6 6 7 8 9 9 9 9 7 7 7 7
+7 7 7 6 6 6 6
+
+Sequence : 22ak93c2.s1t
+Is_read
+Padded
+Staden_id 13
+Clipping QUAL 44 45
+Align_to_SCF 1 822 1 822
+SCF_File 22ak93c2.s1tSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_terminator
+Template 22ak93c2
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 43 "M13mp1"
+
+DNA : 22am84d9.s1
+CAACCCGCACACCTGCACGTCGACTCTAGAGGATCCCCATAAATAAATAAATtAgCTGGG
+TGTGATGTCACATGCCTGTgATCCCAGCTACTTGGGAgGCTGAGACAGAAGGATCGCTTG
+AGTCCAGGAGGTCAAGGCTGTAGCGAGTCATGTTCGCCCCACTGCACTCCAGCCTGGGCG
+ACACAATGAGACCCTGTCTCAAAAACAACAACAACAAAAGGCATCTCTACCTACCTGAGA
+CCTCTTGGTATCGCTAGGCAGAAGCAAACCGCCTGTACTTGTATTAAAGGTCCGGGTCTT
+GGTTTTCACTGGTTCATTTGTTTCCCGATAGAGCTTTATTGGAGGAGGCTTGAGAGCCTT
+CTGGACCAGATTATAAATGCCCACAGAGATCACTATATCTTTGTTGAGCTTCAAGCTTTA
+ACCTGAGGGCAGTAAAAAATGGAAGAGCATGAACTGGCAAAATGCACTTTTCTATCTAAA
+ATTCC
+
+BaseQuality : 22am84d9.s1
+4 9 9 9 9 10 10 9 9 9 9 10 10 10 8 8 6 6 10 8 11 12 15 14 13 15 11 11
+11 12 14 10 10 10 17 23 31 31 21 20 13 21 11 10 10 16 14 14 14 23 11
+11 10 10 10 11 11 12 32 18 23 23 32 32 34 37 34 37 37 38 27 18 18 17
+24 30 32 32 10 10 11 23 23 38 37 37 37 37 37 37 37 37 37 37 32 32 22
+22 22 32 28 40 37 37 37 45 32 32 28 22 22 12 16 16 32 32 37 37 37 37
+28 28 28 32 32 30 30 27 29 29 34 37 37 37 37 37 37 37 37 37 28 22 20
+30 30 29 37 34 37 37 37 37 37 37 37 37 37 37 37 45 45 37 37 37 37 45
+37 37 34 34 34 33 31 33 34 37 27 27 13 13 13 21 32 34 36 31 31 33 19
+17 14 9 9 13 24 27 27 33 33 33 33 33 33 33 33 33 36 36 37 37 37 37 37
+45 45 36 34 34 28 31 31 34 34 37 38 34 38 38 33 30 30 30 30 30 33 34
+37 37 37 34 34 34 34 34 35 35 33 33 33 33 33 33 34 30 30 30 30 30 30
+33 33 30 30 30 34 34 34 34 31 31 31 36 36 34 31 17 13 8 8 15 18 27 31
+28 28 28 28 28 30 31 35 35 34 34 34 34 33 33 33 34 34 38 38 38 37 32
+28 22 19 19 25 27 37 30 30 30 28 28 28 38 37 34 34 37 31 31 25 24 28
+30 30 28 28 28 28 28 28 34 34 34 33 33 31 31 27 24 18 15 18 18 23 23
+28 27 22 18 11 11 16 21 24 33 34 23 23 8 8 9 12 14 17 20 20 20 25 25
+27 27 23 16 11 15 14 9 9 19 17 18 22 18 11 11 11 12 17 18 31 31 31 31
+27 27 33 29 22 18 17 17 20 20 17 13 11 11 11 6 8 14 17 22 22 25 25 20
+20 20 17 14 4 4 4 14 15 21 22 18 11 12 7 7 7 7 7 12 21 18 15 13 9 9 15
+12 8 6 6 6 6 9 6 6 6 12 14 13 13 17 21 21 24 21 22 18 14 18 16 14 14
+10 6 7 6 13 8 8 7
+
+
+Sequence : 22am84d9.s1
+Is_read
+Padded
+Staden_id 7
+Clipping QUAL 39 427
+Align_to_SCF 1 485 1 485
+SCF_File 22am84d9.s1SCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_primer
+Template 22am84d9
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 38 "M13mp18"
+Tag ALUS 41 210
+Tag DONE 53 53 "AUTO-EDIT: replaced C by t at 53 (all_stranded, isolated, strong)"
+Tag DONE 55 55 "AUTO-EDIT: replaced C by g at 55 (all_stranded, isolated, strong)"
+Tag DONE 80 80 "AUTO-EDIT: replaced C by g at 80 (all_stranded, isolated, strong)"
+Tag DONE 98 98 "AUTO-EDIT: replaced C by g at 98 (all_stranded, isolated, strong)"
+Tag AMBG 338 360 "AUTOEDIT: Check this edit cluster!"
+Tag CONF 413 413 "AUTOEDIT: possible compression"
+
+DNA : 22ak66f10.s1lt
+CCCGCGGGGGAGGAGGGAnGGGGGGAGAGGAGnAnAAnnnnnnnnnnnnnAGCCCCAAnn
+nAnnCCCTCTTnnnnnnnnAGACGGGGGGnGCCCTGGGnGnGCnTnnnnnnnnnnTTGGT
+TGAGGTGnCGAGCAAATCTTGCATGCCTGtcCGTCTACTCtAGAGGATCCCACAAATGAA
+CCAGTGAAAACCAAGACCCGGACCTTTAATACAAGTACAGGCGGTTTGCTTCTGCCTAGC
+GATACCAAGAGGTCTCAGGTAGGTAGAGATGCCTTTTGTTGTTGTTGTTTTTGAGACAGG
+GTCTCATTGTGTCGCCCAGGCTGGAGTGCAGTGGGGCGAACATGACTCGCTACAGCCTTG
+ACCTCCTGGACTCAAGCGATCCTTCTGTCTCAGCCTCCCAAGTAGCTGGGATCACAGGCA
+TGTGACATCACACCCAGCTAATTTATTTATTTATTTATTTTTTAAGAGACTGGATCGACT
+GGGCACAGTGGCTCATGCCTGTAATCCCAnCACTTTGGGAGGCCGAGGCAGGTGGATTAC
+CGAGGTCAGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCCCATCTCTACTAAAAA
+TACAAAATGAGCTGGGCATGGTGGTGCATGCCTGTAATCCCAGCTACTCCGGAGGCTGAG
+GCAnGAGAATCCCTTGAACCCGGGAGGCAAAAGTTGCGGTGAGCCGAAATCTCGCCATTG
+CGCTCCAGCCTGGGCAACAAAAGCGTAACTCCGTCTCCnnnnnnnnAAAAAAAGAGACGG
+TGTTTCGCTATGTTGCCTGGGCTGGGTGTCAAACTCCTGGGTCTTAAAGTAATCCCCCCC
+TGCTTTGGGTTCCCCCAAATTGCTGGGGGTGGCTGGGATTCCCGAACTTTGGGGCCACTG
+TTGCCCCAGCCGTTTTTTTGGTTTCGTTTTGGTTTTGGTTTTGTTTTAATAAAAAAATAA
+AGGGTCTCACCTATGTT
+
+BaseQuality : 22ak66f10.s1lt
+6 6 4 6 8 8 11 8 8 6 6 11 4 4 4 12 6 4 4 4 8 7 6 6 6 6 6 6 6 8 4 4 4 4
+4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 7 7 6 6 6 8 4 4 4 4 4 4 4 4 6 4 6 6
+8 4 4 4 4 4 4 4 4 4 4 4 4 6 6 6 6 6 6 4 4 4 6 6 4 4 8 12 4 4 4 4 4 4 4
+4 4 4 4 4 4 4 4 4 4 4 4 6 6 4 4 4 4 4 4 8 6 4 4 4 4 4 4 15 17 8 4 4 6
+9 21 21 30 28 30 33 33 21 18 8 8 8 10 15 23 21 26 26 19 14 7 7 8 8 10
+18 21 18 10 9 9 18 14 24 24 29 32 34 37 34 36 32 32 30 30 30 34 38 44
+45 37 37 34 27 27 15 15 23 32 40 40 37 37 34 45 45 45 45 45 42 25 25
+25 26 26 20 23 28 30 33 33 33 34 34 34 34 37 45 45 45 45 49 49 49 49
+49 45 45 43 38 38 37 37 40 37 37 34 27 27 12 10 18 14 32 27 27 27 29
+29 12 18 10 15 10 21 10 14 10 19 19 41 45 45 45 45 45 40 40 34 37 37
+45 45 45 45 45 45 45 45 45 40 51 37 37 25 22 18 27 22 25 25 29 29 28
+33 33 40 37 37 37 37 37 37 34 37 37 42 35 32 30 30 37 38 45 45 30 30
+30 33 31 33 32 37 45 45 45 32 30 30 37 33 37 37 40 37 37 37 37 37 37
+37 37 37 37 34 34 34 37 45 45 45 45 45 45 45 45 49 45 45 45 45 33 33
+29 29 33 33 33 33 33 37 40 45 45 45 45 45 45 45 35 35 35 35 35 40 38
+45 45 45 37 37 32 22 14 14 14 32 45 45 45 38 37 31 28 27 30 28 27 22
+27 27 31 31 34 31 28 28 27 31 33 33 33 33 33 33 32 28 28 30 34 37 37
+35 35 35 37 37 33 33 33 33 33 33 31 31 30 30 33 31 31 36 33 33 33 34
+25 25 10 10 10 19 19 34 34 34 34 31 31 34 34 36 38 30 30 26 23 30 31
+34 32 45 37 37 37 37 33 33 40 40 40 36 33 31 31 31 33 33 23 22 4 4 4
+18 23 32 34 36 36 32 28 21 21 21 29 20 28 25 24 18 25 21 27 27 32 32
+34 31 31 34 37 37 37 37 32 32 10 21 10 25 28 32 29 34 28 31 27 27 28
+22 22 27 29 32 32 37 37 37 37 37 37 37 37 35 34 34 34 31 27 27 19 19
+30 34 37 38 34 34 34 34 34 40 38 38 38 38 37 37 37 35 35 32 28 28 28
+30 30 30 33 30 30 22 22 22 32 32 35 35 34 34 35 35 34 37 37 38 30 24
+24 24 24 24 31 38 38 32 27 27 27 35 25 18 12 9 9 11 21 23 18 12 6 6 8
+8 8 9 10 17 10 14 8 9 8 7 4 4 4 16 14 14 12 15 8 8 11 15 14 25 25 25
+28 33 33 33 21 29 7 7 7 8 8 7 8 9 9 8 11 24 22 31 25 23 23 15 13 10 7
+9 9 8 8 12 7 7 7 16 11 18 14 25 25 22 19 16 17 14 8 9 10 13 15 27 28
+31 23 22 14 14 13 15 15 23 21 8 8 6 6 6 6 9 18 20 28 22 22 20 17 14 9
+4 4 4 4 4 4 4 4 4 4 16 10 13 20 23 23 12 9 9 9 17 10 9 7 8 6 6 8 4 4 7
+12 13 14 7 8 8 8 8 8 18 21 16 9 10 9 7 9 7 9 8 10 7 7 7 12 9 9 7 7 8 8
+6 6 8 8 8 6 8 6 6 6 6 7 6 11 6 8 6 6 6 6 6 8 9 9 8 9 12 8 8 8 7 8 8 7
+8 6 6 6 9 8 6 6 6 10 13 11 7 9 7 7 7 7 7 8 9 7 7 9 9 7 4 4 4 7 11 7 7
+9 8 7 6 6 6 6 6 8 8 10 9 8 8 6 6 6 6 7 6 6 4 4 4 4 8 6 8 13 16 20 8 8
+7 7 7 7 7 7 7 8 12 8 8 6 6 6 6 6 6 6 6 6 6 9 8 8 6 6 6 8 6 9 14 9 9 8
+9 7 6 6 8 6 6 8 7 6 6 8 6 6 7 7 7 7 7 7 8 8 4 4
+
+
+Sequence : 22ak66f10.s1lt
+Is_read
+Padded
+Staden_id 9
+Clipping QUAL 140 640
+Align_to_SCF 1 977 1 977
+SCF_File 22ak66f10.s1ltSCF
+Primer Universal_primer
+Strand Forward
+Dye Dye_primer
+Template 22ak66f10
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 3 "M13mp18"
+Tag DONE 150 150 "AUTO-EDIT: replaced C by t at 150 (all_stranded, compound, strong)"
+Tag DONE 151 151 "AUTO-EDIT: replaced G by c at 151 (all_stranded, compound, strong)"
+Tag DONE 161 161 "AUTO-EDIT: replaced A by t at 161 (all_stranded, compound, strong)"
+Tag ALUS 283 462
+Tag ALUS 477 767
+
+DNA : 22ak70h4.r1t
+TGCCGCCTTAGATTACAGCTCTACACGGTACCTCTATCGGGAAACAAATGAACCAgTGAA
+AACCAAGACCCGGACCTTTAATACAAGTACAGGCGGTTTGCTTCTGCCTAGCGATACCAA
+GAGGTCTCAGGTAGGTAGAGATGCCTTTTGTTGTTGTTGTTTTTGAGACAGGGTCTCATT
+GTGTCGCCCAgGCTGGAGTGCAGTGGGGCGAACATGACTCGCTACAGCCTTGACCTCCTG
+GACTCAAGCGATCCTTCTGTCTCAGCCTCCCAAGTAGCTGGGATCACAGGCATGTGACAT
+CACACCCAGCTAATTTATTTATTTATTTATTTTTTAAGAGACTGGATCGACTGGGCACAG
+TGGCTCATGCCTGTAATCCCACCACTTTGGGAGGCCGAGGCATGTGGATTACCGAGGTTC
+GGAGTTCAAGACCAGCCTGACCCTTCTGGAGAAACCCCATCTCCACTTTAAATACCAAAT
+GAAGCTGGGCCTGGTGGTGCCTGCCTGTAATCCCCCCTTCTCAGGATGCTGAAGCAGGAC
+ATTCCTTGAACCCCGGAAGCACATGTTGCCGTGACCCAATATCCCCCCTTGTCCCTCACC
+CCGGGCCACCAAACCTTATTCCCTCCCAAATAAAAAAAAAAAAAAACGGGTTCCCTTTTT
+TGCCGGGGGGGGTTTCTACCCC
+
+BaseQuality : 22ak70h4.r1t
+4 4 4 4 4 8 6 6 8 8 12 6 7 11 13 12 6 6 6 6 6 6 9 10 10 10 21 21 26 31
+27 27 13 14 11 11 11 12 19 20 27 18 18 23 23 16 10 10 14 28 18 18 11
+11 9 9 9 10 10 10 21 21 27 23 18 10 9 9 14 18 32 31 37 34 28 28 22 19
+19 27 24 21 18 17 9 9 12 19 24 28 30 30 30 33 33 30 30 37 40 40 45 37
+41 41 44 44 45 32 42 37 32 32 32 32 37 31 31 33 21 21 11 10 22 18 32
+24 27 27 27 27 11 14 10 23 12 23 9 10 10 18 11 25 25 34 45 37 37 34 34
+34 34 34 34 34 33 33 33 34 34 34 34 33 34 28 31 25 21 23 21 21 13 13
+15 15 17 23 21 26 26 31 28 32 23 23 12 22 25 21 19 9 8 8 17 21 23 23
+20 18 17 27 23 21 27 30 38 38 28 28 17 17 13 18 19 27 25 25 28 28 28
+28 25 31 26 26 18 15 13 9 9 15 19 23 25 31 36 36 36 36 36 36 36 36 24
+19 12 13 18 24 31 31 29 29 31 29 29 33 36 40 44 36 33 34 34 37 36 36
+45 45 34 30 28 28 28 22 32 24 22 22 32 32 36 37 27 27 14 25 25 29 23
+22 19 17 19 19 24 25 27 24 18 18 16 16 16 24 22 30 30 28 28 30 27 27
+23 20 22 25 25 25 21 22 21 16 14 19 17 17 19 20 17 11 11 11 11 12 24
+24 22 20 34 17 17 23 23 31 34 27 27 17 13 15 27 27 34 31 31 24 22 19
+16 16 15 32 23 27 18 9 9 9 13 9 14 20 22 21 21 19 17 14 8 6 6 8 8 9 11
+14 8 7 11 15 14 9 9 9 12 8 10 6 8 8 6 4 4 4 14 15 23 21 13 13 11 13 11
+11 6 8 8 6 6 6 7 6 6 6 6 7 6 6 11 15 17 27 20 17 17 13 21 13 14 4 4 8
+6 6 6 6 8 6 8 11 7 8 8 10 10 19 24 16 16 9 9 12 7 7 8 8 8 8 6 6 11 15
+11 10 9 9 11 14 15 11 8 7 7 7 7 12 9 10 10 8 8 14 10 10 18 21 21 18 16
+10 9 11 9 9 14 10 10 8 8 9 8 8 12 14 10 10 11 11 10 9 8 7 7 7 7 4 4 4
+12 10 14 10 8 14 6 6 6 7 6 6 6 6 6 10 8 9 7 7 7 9 14 15 10 10 10 10 9
+8 13 8 4 4 4 4 4 6 6 12 6 6 10 8 8 8 6 9 12 7 6 6 6 8 8 9 7 7 7 8 9 8
+8 6 6 6 6 6 4 6 6 6 6 7 8 8 6 6 8 8 8 8 9 9 8 10 11 9 8 8 8 8 6 6 11 9
+9 10 9 12 8 6 6 6 10 12 10 10 8 8 8 7 10 13 8 13 19 18 13 6 6 6 6 8 6
+7 12 7 7 10 10 7 7 7 7 7 7 9 13 6 10 7 7 12 11 7 6 6 6 6 8 8 8 4
+
+
+Sequence : 22ak70h4.r1t
+Is_read
+Padded
+Staden_id 15
+Clipping QUAL 28 377
+Align_to_SCF 1 682 1 682
+SCF_File 22ak70h4.r1tSCF
+Primer Universal_primer
+Strand Reverse
+Dye Dye_terminator
+Template 22ak70h4
+Clone bK216E10
+Sequencing_vector "m13mp18"
+Seq_vec SVEC 1 27 "M13mp18"
+Tag DONE 56 56 "AUTO-EDIT: replaced C by g at 56 (all_stranded, isolated, strong)"
+Tag ALUS 155 334
+Tag DONE 191 191 "AUTO-EDIT: replaced T by g at 191 (all_stranded, isolated, strong)"
+Tag ALUS 349 579
+
+Sequence : Contig2
+Is_contig
+Padded
+Assembled_from 22ak93c2.r1t 1 293 39 331
+Assembled_from 22ak93c1.r1ta 1 305 31 335
+Assembled_from 22am91h5.r1t 110 439 32 361
+Assembled_from 22aq40a8.s1 221 570 39 388
+Assembled_from 22an02b10.s1t 252 666 32 446
+Assembled_from 22ak67d6.r1t 260 522 30 292
+Assembled_from 22ak64b1.s1t 331 707 40 416
+Assembled_from 22ak81h10.s1t 331 713 36 418
+Assembled_from 22ak66c6.s1t 337 848 37 548
+Assembled_from 22ak93c1.s1lt 1024 482 56 598
+Assembled_from 22ak64e2.s1t 866 487 41 420
+Assembled_from 22ak70f10.s1t 554 1027 35 508
+Assembled_from 22ak92c4.s1t 635 1088 37 490
+Assembled_from 22ap08f1.s1t 639 777 17 155
+Assembled_from 22ai01c4.s1t 682 1200 35 553
+Assembled_from 22ak93c1.s1t 1036 735 44 345
+Assembled_from 22ak65e10.s1t 1499 966 42 575
+Assembled_from 22ak93c2.s1t 1036 1035 44 45
+Assembled_from 22am84d9.s1 1488 1100 39 427
+Assembled_from 22ak66f10.s1lt 1174 1674 140 640
+Assembled_from 22ak70h4.r1t 1190 1539 28 377
+
+DNA : Contig2
+TTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTATAnACTGTGCGTGCGC
+CACCATGCCTGGCTAATTTTTGTATTTTTAGTAGGGATGGGGTTTCACCATGTTGGCCAA
+GCTGGTCTCGAGCTCCTGACCTAGGATTACAGGCCTAAGCCACCGCACCCGGCATGATGG
+GTCTTTATTCTTCAAAGCAGGAGGAAGGGATCCTAGAAAAACAGAGACAAGGCCAAACAT
+GGTAGCTCACACCTGTAATnnnnnCACTTTGGGAGGCCAGTGCGGGTGAATCACGAnGTC
+AGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAnCACCGTCTCTACTAAAATAAAAAGA
+AATTAGCTGGGTGTCGTGGCAGGTGCnTGTAATCCCAGCCACTTGGGAAGCTGAGGCAGG
+AGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCGACTGCACTC
+CAGCCCAACCAATAGTGTGAGACTCTGTCTCGAAAAAAAAAAAGCAGAGACAAGACnACT
+AGTACAGTACTTACAGGGTTATTATGATGATTAAATGAGAGAATAGCTGTGAGGTGATTG
+ATATAGTGCTGTGCTTAATACAAACTATCATTTTATTATACGGGTTGAGTGTnTCTAATC
+TGAAAATCCAAAATTAGAAATGCTCTACAGTCTGAAACTTTTTTGAGCACCGACCTAATG
+TTCAAAGGAAGTGCTTATTGGAGCATTATGGGTTGTTAGATTTTTGGGTTGGGAATATTC
+AACCAGTAAGTACTATAAAATGCAAATATTCCAAAAAAAATCTGAAATCTGAAACATTTC
+TGGTCCTAAGCAAGCATTTTGCAAAGGGATACGCAACCTGTAGTACGTTCTTTATCATTG
+TTTTAAGTAGTTAATATATTGTGGTACAGATTCTGAGGTGGTATAGCAAATTCGATTGTA
+TTATTAAAAAGCATATTTATATTTTGAGAGCTTGCTTAGGATTATTGGAGAGAATAAAAC
+AGTGAAGCTTTGGTGTTATGAGGGAATTTTAGATAGAAAAGTGCAGTTTTTCAGTTCATG
+CTCTTTCATTTTTTACTCCCTCAGGTTAAAGCTnGAAGCTCAACAAAGATATAGTGATCT
+CTGTGGGCATTTATAATCTGGTCCAGAAGGCTCTnnAnnCnnnTCCnnnnnnnCTnnAnn
+nnnnnACAAATGAACCAGTGAAAACCAAGACCCGGACCTTTAATACAAGTACAGGCGGTT
+TGCTTCTGCCTAGCGATACCAAGAGGTCTCAGGTAGGTAGAGATGCCTTTTGTTGTTGTT
+GTTTTTGAGACAGGGTCTCATTGTGTCGCCCAGGCTGGAGTGCAGTGGGGCGAACATGAC
+TCGCTACAGCCTTGACCTCCTGGACTCAAGCGATCCTTCTGTCTCAGCCTCCCAAGTAGC
+TGGGATCACAGGCATGTGACATCACACCCAGCTAATTTATTTATTTATTTATTTTTTAAG
+AGACTGGATCGACTGGGCACAGTGGCTCATGCCTGTAATCCCAnCACTTTGGGAGGCCGA
+GGCAGGTGGATTACCGAGGTCAGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCCC
+ATCTCTACTAAAAATACAAAATGAGCTGGGCATGGTGGTGCATGCCTGTAATCC
+
+BaseQuality : Contig2
+4 4 8 4 6 10 13 21 24 25 33 33 33 30 27 21 15 27 19 30 30 33 33 30 21
+21 10 9 17 11 27 27 37 38 33 35 35 35 35 44 45 45 38 37 37 45 45 45 43
+43 43 45 45 45 45 45 45 45 21 21 23 30 30 34 37 37 38 45 32 45 37 41
+37 45 30 45 45 34 34 34 32 29 22 32 32 45 45 45 33 28 28 37 37 34 34
+34 35 34 34 34 34 34 34 37 37 40 41 37 34 37 37 37 32 24 22 27 29 25
+27 20 21 21 21 27 45 41 40 40 40 42 34 34 37 41 45 51 45 45 37 30 37
+41 41 37 36 28 30 30 30 22 33 33 35 33 33 41 51 51 45 39 39 39 30 28
+33 34 34 39 34 41 34 34 41 33 33 39 39 39 33 33 33 30 33 33 33 30 29
+19 19 24 25 32 33 45 36 36 36 36 41 41 30 30 30 37 37 43 51 51 51 51
+45 37 37 30 37 37 37 51 51 51 51 51 37 37 28 28 10 10 10 13 10 10 10 9
+9 9 21 21 28 37 37 37 33 33 33 33 33 33 34 34 34 33 33 33 33 21 25 25
+22 22 29 29 33 33 33 33 31 31 27 28 17 23 23 28 26 24 32 10 10 10 15
+15 26 32 32 37 45 45 32 45 33 26 24 32 37 37 35 34 37 35 37 34 37 37
+40 37 37 45 45 38 38 45 45 40 37 37 37 34 30 30 28 28 28 30 37 37 34
+34 34 34 34 33 31 31 25 21 19 22 21 25 30 32 32 30 22 22 25 28 24 25
+26 17 17 23 27 27 33 31 31 34 38 38 38 38 34 26 17 15 8 8 11 16 25 31
+32 33 29 26 32 29 33 34 34 34 36 33 34 34 31 27 31 34 38 45 45 36 37
+37 37 36 36 38 34 32 28 25 22 22 21 17 18 29 29 23 23 22 27 25 20 18
+11 11 15 15 16 23 21 17 19 24 24 24 31 31 31 33 33 33 31 33 33 29 29
+29 29 29 31 31 31 23 24 19 12 10 16 10 10 20 10 10 13 15 30 23 29 23
+28 18 10 10 16 21 18 19 19 24 24 12 11 9 9 10 21 23 31 31 33 28 28 14
+17 17 28 21 30 24 30 28 22 26 23 19 10 10 12 23 22 23 19 12 10 9 9 10
+19 24 29 30 34 34 34 34 34 29 25 25 32 26 31 31 32 20 17 15 12 12 4 4
+4 17 23 33 30 24 18 13 18 16 12 16 8 9 9 19 15 15 4 4 4 7 11 6 6 6 7 8
+9 17 12 13 19 23 25 13 13 13 19 21 29 32 30 26 20 20 12 9 9 8 9 8 8 15
+24 17 15 8 8 9 17 16 4 4 4 8 8 13 15 23 14 9 8 8 10 10 17 23 21 17 12
+13 22 20 19 15 11 11 9 8 8 8 8 8 8 10 9 9 7 8 8 10 12 10 10 12 4 4 4 4
+4 4 10 10 10 10 10 9 8 8 7 8 9 11 9 9 4
+
+