zarrify 0.0.1__py3-none-any.whl

zarrify/__about__.py

@@ -0,0 +1,3 @@
+# SPDX-FileCopyrightText: 2025-present Yurii Zubov <zubov452@gmail.com>
+
+__version__ = "0.0.1"

zarrify/__init__.py

@@ -0,0 +1,8 @@
+# SPDX-FileCopyrightText: 2025-present Yurii Zubov <zubov452@gmail.com>
+#
+# SPDX-License-Identifier: MIT
+
+
+from zarrify import to_zarr
+
+__all__ = ["to_zarr"]

zarrify/formats/mrc.py

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+import zarr
+import mrcfile
+import os
+from typing import Tuple
+from dask.array.core import slices_from_chunks, normalize_chunks
+from dask.distributed import Client, wait
+from toolz import partition_all
+import time
+from zarrify.utils.volume import Volume
+
+
+class Mrc3D(Volume):
+
+    def __init__(
+        self,
+        src_path: str,
+        axes: list[str],
+        scale: list[float],
+        translation: list[float],
+        units: list[str],
+    ):
+        """Construct all the necessary attributes for the proper conversion of tiff to OME-NGFF Zarr.
+
+        Args:
+            input_filepath (str): path to source tiff file.
+        """
+        super().__init__(src_path, axes, scale, translation, units)
+
+        self.memmap = mrcfile.mmap(self.src_path, mode="r")
+        self.ndim = self.memmap.data.ndim
+        self.shape = self.memmap.shape
+        self.dtype = self.memmap.data.dtype
+
+    def save_chunk(self, z_arr: zarr.Array, chunk_slice: Tuple[slice, ...]):
+        """Copies data from a particular part of the input mrc array into a specific chunk of the output zarr array.
+
+        Args:
+            z_arr (zarr.core.Array): output zarr array object
+            chunk_slice (Tuple[slice, ...]): slice of the mrc array to copy.
+        """
+        mrc_file = mrcfile.mmap(self.src_path, mode="r")
+
+        if not (mrc_file.data[chunk_slice] == 0).all():
+            z_arr[chunk_slice] = mrc_file.data[chunk_slice]
+
+    def write_to_zarr(
+        self,
+        z_arr: zarr.Array,
+        client: Client,
+    ):
+        """Use mrcfile memmap to access small parts of the mrc file and write them into zarr chunks.
+
+        Args:
+            dest_path (str): path to the zarr group where the output dataset is stored.
+            client (Client): instance of a dask client
+        """
+
+        out_slices = slices_from_chunks(
+            normalize_chunks(z_arr.chunks, shape=z_arr.shape)
+        )
+        out_slices_partitioned = tuple(partition_all(100000, out_slices))
+
+        for idx, part in enumerate(out_slices_partitioned):
+
+            print(f"{idx + 1} / {len(out_slices_partitioned)}")
+            start = time.time()
+            fut = client.map(lambda v: self.save_chunk(z_arr, v), part)
+            print(
+                f"Submitted {len(part)} tasks to the scheduler in {time.time()- start}s"
+            )
+            # wait for all the futures to complete
+            result = wait(fut)
+            print(f"Completed {len(part)} tasks in {time.time() - start}s")

zarrify/formats/n5.py

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+import zarr
+import os
+
+class N53D(Volume):
+    def __init__(
+        self,
+        src_path: str,
+        axes: list[str],
+        scale: list[float],
+        translation: list[float],
+        units: list[str],
+    ):
+        """Construct all the necessary attributes for the proper conversion of tiff to OME-NGFF Zarr.
+
+        Args:
+            input_filepath (str): path to source tiff file.
+        """
+        super().__init__(src_path, axes, scale, translation, units)
+        self.store_path, self.arr_path = self.separate_store_path(src_path, '')
+        self.n5_store = zarr.N5Store(self.store_path)
+        self.n5_arr = zarr.open(store = self.n5_store, path=self.arr_path, mode='r')
+
+        self.shape = self.n5_arr.shape
+        self.dtype = self.n5_arr.dtype
+        self.chunks = self.n5_arr.chunks
+        
+    def separate_store_path(store, path):
+        """
+        sometimes you can pass a total os path to node, leading to
+        an empty('') node.path attribute.
+        the correct way is to separate path to container(.n5, .zarr)
+        from path to array within a container.
+
+        Args:
+            store (string): path to store
+            path (string): path array/group (.n5 or .zarr)
+
+        Returns:
+            (string, string): returns regularized store and group/array path
+        """
+        new_store, path_prefix = os.path.split(store)
+        if ".n5" in path_prefix:
+            return store, path
+        return separate_store_path(new_store, os.path.join(path_prefix, path))

zarrify/formats/tiff.py

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+from tifffile import imread
+import numpy as np
+import zarr
+import os
+from dask.distributed import Client, wait
+import time
+import dask.array as da
+import copy
+from zarrify.utils.volume import Volume
+
+
+class Tiff3D(Volume):
+
+    def __init__(
+        self,
+        src_path: str,
+        axes: list[str],
+        scale: list[float],
+        translation: list[float],
+        units: list[str],
+    ):
+        """Construct all the necessary attributes for the proper conversion of tiff to OME-NGFF Zarr.
+
+        Args:
+            input_filepath (str): path to source tiff file.
+        """
+        super().__init__(src_path, axes, scale, translation, units)
+
+        self.zarr_store = imread(os.path.join(src_path), aszarr=True)
+        self.zarr_arr = zarr.open(self.zarr_store)
+
+        self.shape = self.zarr_arr.shape
+        self.dtype = self.zarr_arr.dtype
+
+    def write_to_zarr(self, zarray: zarr.Group, client: Client):
+        chunks_list = np.arange(0, zarray.shape[0], zarray.chunks[0])
+
+        src_path = copy.copy(self.src_path)
+
+        start = time.time()
+        fut = client.map(
+            lambda v: write_volume_slab_to_zarr(v, zarray, src_path), chunks_list
+        )
+        print(
+            f"Submitted {len(chunks_list)} tasks to the scheduler in {time.time()- start}s"
+        )
+
+        # wait for all the futures to complete
+        result = wait(fut)
+        print(f"Completed {len(chunks_list)} tasks in {time.time() - start}s")
+
+        return 0
+
+
+def write_volume_slab_to_zarr(chunk_num: int, zarray: zarr.Array, src_path: str):
+
+    # check if the slab is at the array boundary or not
+    if chunk_num + zarray.chunks[0] > zarray.shape[0]:
+        slab_thickness = zarray.shape[0] - chunk_num
+    else:
+        slab_thickness = zarray.chunks[0]
+
+    slab_shape = [slab_thickness] + list(zarray.shape[-2:])
+    np_slab = np.empty(slab_shape, zarray.dtype)
+
+    tiff_slab = imread(src_path, key=range(chunk_num, chunk_num + slab_thickness, 1))
+    np_slab[0 : zarray.chunks[0], :, :] = tiff_slab
+
+    # write a tiff stack slab into zarr array
+    zarray[chunk_num : chunk_num + zarray.chunks[0], :, :] = np_slab

zarrify/formats/tiff_stack.py

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+from tifffile import imread
+import numpy as np
+import zarr
+import os
+from dask.distributed import Client, wait
+import time
+import dask.array as da
+from natsort import natsorted
+from glob import glob
+from zarrify.utils.volume import Volume
+
+
+class TiffStack(Volume):
+
+    def __init__(
+        self,
+        src_path: str,
+        axes: list[str],
+        scale: list[float],
+        translation: list[float],
+        units: list[str],
+    ):
+        """Construct all the necessary attributes for the proper conversion of tiff to OME-NGFF Zarr.
+
+        Args:
+            input_filepath (str): path to source tiff file.
+        """
+        super().__init__(src_path, axes, scale, translation, units)
+
+        self.stack_list = natsorted(glob(os.path.join(src_path, "*.tif*")))
+        probe_image_store = imread(
+            os.path.join(src_path, self.stack_list[0]), aszarr=True
+        )
+        probe_image_arr = da.from_zarr(probe_image_store)
+
+        self.dtype = probe_image_arr.dtype
+        self.shape = [len(self.stack_list)] + list(probe_image_arr.shape)
+
+    def write_tile_slab_to_zarr(
+        self, chunk_num: int, zarray: zarr.Array, src_volume: list
+    ):
+
+        # check if the slab is at the array boundary or not
+        if chunk_num + zarray.chunks[0] > zarray.shape[0]:
+            slab_thickness = zarray.shape[0] - chunk_num
+        else:
+            slab_thickness = zarray.chunks[0]
+
+        slab_shape = [slab_thickness] + list(zarray.shape[-2:])
+        np_slab = np.empty(slab_shape, zarray.dtype)
+
+        # combine tiles into a slab with thickness equal to the chunk size in z direction
+        for slab_index in np.arange(chunk_num, chunk_num + slab_thickness, 1):
+            try:
+                image_tile = imread(src_volume[slab_index])
+            except:
+                print(
+                    f"Tiff tile with index {slab_index} is not present in tiff stack."
+                )
+            np_slab[slab_index - chunk_num, :, :] = image_tile
+
+        # write a tiff stack slab into a zarr array
+        zarray[chunk_num : chunk_num + zarray.chunks[0], :, :] = np_slab
+
+    # parallel writing of tiff stack into zarr array
+    def write_to_zarr(self, zarray: zarr.Array, client: Client):
+        chunks_list = np.arange(0, zarray.shape[0], zarray.chunks[0])
+
+        start = time.time()
+        fut = client.map(
+            lambda v: self.write_tile_slab_to_zarr(v, zarray, self.stack_list),
+            chunks_list,
+        )
+        print(
+            f"Submitted {len(chunks_list)} tasks to the scheduler in {time.time()- start}s"
+        )
+
+        # wait for all the futures to complete
+        result = wait(fut)
+        print(f"Completed {len(chunks_list)} tasks in {time.time() - start}s")
+
+        return 0

zarrify/to_zarr.py

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+import zarr
+from numcodecs import Zstd
+import os
+import click
+import sys
+from dask.distributed import Client
+import time
+from zarrify.formats.tiff_stack import TiffStack
+from zarrify.formats.tiff import Tiff3D
+from zarrify.formats.mrc import Mrc3D
+from zarrify.formats.n5 import N53D
+from zarrify.utils.dask_utils import initialize_dask_client
+
+
+# @click.command("zarrify")
+# @click.option(
+#     "--src",
+#     "-s",
+#     type=click.Path(exists=True),
+#     help="Input file/directory location",
+# )
+# @click.option("--dest", "-s", type=click.STRING, help="Output .zarr file path.")
+# @click.option(
+#     "--num_workers", "-w", default=100, type=click.INT, help="Number of dask workers"
+# )
+# @click.option(
+#     "--cluster",
+#     "-c",
+#     default="",
+#     type=click.STRING,
+#     help="Which instance of dask client to use. Local client - 'local', cluster 'lsf'",
+# )
+# @click.option(
+#     "--zarr_chunks",
+#     "-zc",
+#     nargs=3,
+#     default=(64, 128, 128),
+#     type=click.INT,
+#     help="Chunk size for (z, y, x) axis order. z-axis is normal to the tiff stack plane. Default (64, 128, 128)",
+# )
+# @click.option(
+#     "--axes",
+#     "-a",
+#     nargs=3,
+#     default=("z", "y", "x"),
+#     type=str,
+#     help="Metadata axis names. Order matters. \n Example: -a z y x",
+# )
+# @click.option(
+#     "--translation",
+#     "-t",
+#     nargs=3,
+#     default=(0.0, 0.0, 0.0),
+#     type=float,
+#     help="Metadata translation(offset) value. Order matters. \n Example: -t 1.0 2.0 3.0",
+# )
+# @click.option(
+#     "--scale",
+#     "-s",
+#     nargs=3,
+#     default=(1.0, 1.0, 1.0),
+#     type=float,
+#     help="Metadata scale value. Order matters. \n Example: -s 1.0 2.0 3.0",
+# )
+# @click.option(
+#     "--units",
+#     "-u",
+#     nargs=3,
+#     default=("nanometer", "nanometer", "nanometer"),
+#     type=str,
+#     help="Metadata unit names. Order matters. \n Example: -t nanometer nanometer nanometer",
+# )
+# def cli(src, dest, num_workers, cluster, zarr_chunks, axes, translation, scale, units):
+
+    # create a dask client to submit tasks
+#client = initialize_dask_client(cluster)
+
+def to_zarr(src : str,
+            dest: str,
+            client : Client,
+            num_workers : int = 20,
+            zarr_chunks : list[int] = [128]*3,
+            axes : list[str] = ('z', 'y', 'x'), 
+            scale : list[float] = [1.0]*3,
+            translation : list[float] = [0.0]*3,
+            units: list[str] = ['nanometer']*3):   
+    if '.n5' in src:
+        dataset = N53D(src, axes, scale, translation, units)
+    if src.endswith(".mrc"):
+        dataset = Mrc3D(src, axes, scale, translation, units)
+    elif src.endswith(".tif") or src.endswith(".tiff"):
+        dataset = Tiff3D(src, axes, scale, translation, units)
+    if os.path.isdir(src):
+        dataset = TiffStack(src, axes, scale, translation, units)
+
+    z_store = zarr.NestedDirectoryStore(dest)
+    z_root = zarr.open(store=z_store, mode="a")
+    z_arr = z_root.require_dataset(
+        name="s0",
+        shape=dataset.shape,
+        dtype=dataset.dtype,
+        chunks=zarr_chunks,
+        compressor=Zstd(level=6),
+    )
+
+    # write in parallel to zarr using dask
+    client.cluster.scale(num_workers)
+    dataset.write_to_zarr(z_arr, client)
+    client.cluster.scale(0)
+    # populate zarr metadata
+    dataset.add_ome_metadata(z_root)
+
+
+# if __name__ == "__main__":
+#     cli()

zarrify/utils/dask_utils.py

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+from dask_jobqueue import LSFCluster
+from dask.distributed import Client, LocalCluster
+import os
+import sys
+
+
+def initialize_dask_client(cluster_type: str) -> Client:
+    """Initialize dask client.
+
+    Args:
+        cluster_type (str): type of the cluster, either local or lsf
+
+    Returns:
+        (Client): instance of a dask client
+    """
+    if cluster_type == "":
+        print("Did not specify which instance of the dask client to use!")
+        sys.exit(0)
+    elif cluster_type == "lsf":
+        num_cores = 1
+        cluster = LSFCluster(
+            cores=num_cores,
+            processes=num_cores,
+            memory=f"{15 * num_cores}GB",
+            ncpus=num_cores,
+            mem=15 * num_cores,
+            walltime="48:00",
+            local_directory="/scratch/$USER/",
+        )
+    elif cluster_type == "local":
+        cluster = LocalCluster()
+
+    client = Client(cluster)
+    with open(
+        os.path.join(os.getcwd(), "dask_dashboard_link" + ".txt"), "w"
+    ) as text_file:
+        text_file.write(str(client.dashboard_link))
+    print(client.dashboard_link)
+    return client

zarrify/utils/volume.py

@@ -0,0 +1,54 @@
+import zarr
+
+
+class Volume:
+
+    def __init__(
+        self,
+        src_path: str,
+        axes: list[str],
+        scale: list[float],
+        translation: list[float],
+        units: list[str],
+    ):
+        self.src_path = src_path
+        self.metadata = {
+            "axes": axes,
+            "translation": translation,
+            "scale": scale,
+            "units": units,
+        }
+
+    def add_ome_metadata(self, root: zarr.Group):
+        """Add selected tiff metadata to zarr attributes file (.zattrs).
+
+        Args:
+            root (zarr.Group): root group of the output zarr array
+        """
+        # json template for a multiscale structure
+        z_attrs: dict = {"multiscales": [{}]}
+        z_attrs["multiscales"][0]["axes"] = [
+            {"name": axis, "type": "space", "unit": unit}
+            for axis, unit in zip(self.metadata["axes"], self.metadata["units"])
+        ]
+        z_attrs["multiscales"][0]["coordinateTransformations"] = [
+            {"scale": [1.0, 1.0, 1.0], "type": "scale"}
+        ]
+        z_attrs["multiscales"][0]["datasets"] = [
+            {
+                "coordinateTransformations": [
+                    {"scale": self.metadata["scale"], "type": "scale"},
+                    {
+                        "translation": self.metadata["translation"],
+                        "type": "translation",
+                    },
+                ],
+                "path": list(root.array_keys())[0],
+            }
+        ]
+
+        z_attrs["multiscales"][0]["name"] = "/" if root.path == "" else root.path
+        z_attrs["multiscales"][0]["version"] = "0.4"
+
+        # add multiscale template to .attrs
+        root.attrs["multiscales"] = z_attrs["multiscales"]

zarrify-0.0.1.dist-info/LICENSE.txt

@@ -0,0 +1,28 @@
+BSD 3-Clause License
+
+Copyright (c) 2024, Howard Hughes Medical Institute
+
+Redistribution and use in source and binary forms, with or without
+modification, are permitted provided that the following conditions are met:
+
+1. Redistributions of source code must retain the above copyright notice, this
+   list of conditions and the following disclaimer.
+
+2. Redistributions in binary form must reproduce the above copyright notice,
+   this list of conditions and the following disclaimer in the documentation
+   and/or other materials provided with the distribution.
+
+3. Neither the name of the copyright holder nor the names of its
+   contributors may be used to endorse or promote products derived from
+   this software without specific prior written permission.
+
+THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS"
+AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE
+IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
+DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR CONTRIBUTORS BE LIABLE
+FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL
+DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR
+SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER
+CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY,
+OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE
+OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

zarrify-0.0.1.dist-info/METADATA

@@ -0,0 +1,23 @@
+Metadata-Version: 2.3
+Name: zarrify
+Version: 0.0.1
+Summary: 
+Author: Yurii Zubov
+Author-email: zubov452@gmail.com
+Requires-Python: >=3.11
+Classifier: Programming Language :: Python :: 3
+Classifier: Programming Language :: Python :: 3.11
+Classifier: Programming Language :: Python :: 3.12
+Classifier: Programming Language :: Python :: 3.13
+Requires-Dist: click (>=8.1.8,<9.0.0)
+Requires-Dist: colorama (>=0.4.6,<0.5.0)
+Requires-Dist: dask (>=2024.12.1,<2025.0.0)
+Requires-Dist: dask-jobqueue (==0.8.2)
+Requires-Dist: imagecodecs (>=2024.12.30,<2025.0.0)
+Requires-Dist: mrcfile (>=1.5.3,<2.0.0)
+Requires-Dist: natsort (>=8.4.0,<9.0.0)
+Requires-Dist: tifffile (>=2025.1.10,<2026.0.0)
+Requires-Dist: zarr (==2.16.1)
+Description-Content-Type: text/markdown
+
+

zarrify-0.0.1.dist-info/RECORD

@@ -0,0 +1,14 @@
+zarrify/__about__.py,sha256=HPktDE8PNXBOjJb_lSg6ji4H2nkvEFYUlj4x6RAUgvE,95
+zarrify/__init__.py,sha256=qQat7kir84KIAzcPbg_YDcmVt8AX2M1jhi6EYM7MTG0,157
+zarrify/formats/mrc.py,sha256=-_jpEd9cz86ydZJPY8RPrN0RYcJl4GjeGKd006tYJBo,2495
+zarrify/formats/n5.py,sha256=90HLdCC3IqgY78WEf-AMClqDKwzfXjpn512BYhXl0Zs,1521
+zarrify/formats/tiff.py,sha256=QuNcdtcOsg1iGdEMMHl1uNkY8lcx-Xv25QKolZmxkOU,2144
+zarrify/formats/tiff_stack.py,sha256=NtHn-2XIzNyRBH6MrxtXCdfi1C3wV_7JKdn_2cOb6eI,2761
+zarrify/to_zarr.py,sha256=1fZyU6d1hPMS_g4V3XhN08b9daR7uFn5G9iIRwR9wgs,3385
+zarrify/utils/dask_utils.py,sha256=BC3M5Fd0tOEoSaanHL0WCGpwxNkboxQDUTr9ROBiq_o,1108
+zarrify/utils/volume.py,sha256=dTSkodL2utp_zVDBxcS4HfidE80_ISl32x7Vp4M0s3g,1717
+zarrify-0.0.1.dist-info/LICENSE.txt,sha256=CGTDUwAd3nAjOp8LJaPGfTzWEzTnqfLYCulsMyw8B_s,1517
+zarrify-0.0.1.dist-info/METADATA,sha256=H7Gadh1uFyFIywlfAQCGv962i1Bhqp245qZ377Tp1iE,754
+zarrify-0.0.1.dist-info/WHEEL,sha256=RaoafKOydTQ7I_I3JTrPCg6kUmTgtm4BornzOqyEfJ8,88
+zarrify-0.0.1.dist-info/entry_points.txt,sha256=bLnFtFGlpKYvDMeVDTU3LSM3SEEPhWyuU2sx8-k5vKg,47
+zarrify-0.0.1.dist-info/RECORD,,

zarrify-0.0.1.dist-info/WHEEL

@@ -0,0 +1,4 @@
+Wheel-Version: 1.0
+Generator: poetry-core 2.0.0
+Root-Is-Purelib: true
+Tag: py3-none-any

zarrify-0.0.1.dist-info/entry_points.txt

@@ -0,0 +1,3 @@
+[console_scripts]
+zarrify=zarrify.to_zarr:cli
+