spacr 0.3.81__py3-none-any.whl → 0.4.0__py3-none-any.whl

spacr/__init__.py

@@ -67,8 +67,4 @@ logging.basicConfig(filename='spacr.log', level=logging.INFO,
 
 from .utils import download_models
 
-# Check if models already exist
-#models_dir = os.path.join(os.path.dirname(__file__), 'resources', 'models', 'cp')
-#if not os.path.exists(models_dir) or not os.listdir(models_dir):
-#    print("Models not found, downloading...")
 download_models()

spacr/core.py

@@ -7,15 +7,20 @@ from IPython.display import display
 import warnings
 warnings.filterwarnings("ignore", message="3D stack used, but stitch_threshold=0 and do_3D=False, so masks are made per plane only")
 
-def preprocess_generate_masks(src, settings={}):
+def preprocess_generate_masks(settings):
 
     from .io import preprocess_img_data, _load_and_concatenate_arrays
     from .plot import plot_image_mask_overlay, plot_arrays
-    from .utils import _pivot_counts_table, check_mask_folder, adjust_cell_masks, print_progress, save_settings
+    from .utils import _pivot_counts_table, check_mask_folder, adjust_cell_masks, print_progress, save_settings, delete_intermedeate_files
     from .settings import set_default_settings_preprocess_generate_masks
-
-    if not isinstance(settings['src'], (str, list)):
-        ValueError(f'src must be a string or a list of strings')
+    
+    
+    if 'src' in settings:
+        if not isinstance(settings['src'], (str, list)):
+            ValueError(f'src must be a string or a list of strings')
+            return
+    else:
+        ValueError(f'src is a required parameter')
         return
     
     if isinstance(settings['src'], str):
@@ -27,9 +32,8 @@ def preprocess_generate_masks(src, settings={}):
             print(f'Processing folder: {source_folder}')
             settings['src'] = source_folder
             src = source_folder
-            settings = set_default_settings_preprocess_generate_masks(src, settings)
-            
-            save_settings(settings, name='gen_mask')
+            settings = set_default_settings_preprocess_generate_masks(settings)
+            save_settings(settings, name='gen_mask_settings')
 
             if not settings['pathogen_channel'] is None:
                 custom_model_ls = ['toxo_pv_lumen','toxo_cyto']
@@ -158,6 +162,10 @@ def preprocess_generate_masks(src, settings={}):
                     
             torch.cuda.empty_cache()
             gc.collect()
+            
+            if settings['delete_intermediate']:
+                delete_intermedeate_files(settings)
+
             print("Successfully completed run")
     return
 
@@ -172,8 +180,10 @@ def generate_cellpose_masks(src, settings, object_type):
     gc.collect()
     if not torch.cuda.is_available():
         print(f'Torch CUDA is not available, using CPU')
-
-    settings = set_default_settings_preprocess_generate_masks(src, settings)
+        
+    settings['src'] = src
+    
+    settings = set_default_settings_preprocess_generate_masks(settings)
 
     if settings['verbose']:
         settings_df = pd.DataFrame(list(settings.items()), columns=['setting_key', 'setting_value'])
@@ -192,11 +202,13 @@ def generate_cellpose_masks(src, settings, object_type):
         timelapse_objects = settings['timelapse_objects']
     
     batch_size = settings['batch_size']
+    
     cellprob_threshold = settings[f'{object_type}_CP_prob']
 
     flow_threshold = settings[f'{object_type}_FT']
 
     object_settings = _get_object_settings(object_type, settings)
+    
     model_name = object_settings['model_name']
     
     cellpose_channels = _get_cellpose_channels(src, settings['nucleus_channel'], settings['pathogen_channel'], settings['cell_channel'])
@@ -473,7 +485,7 @@ def generate_image_umap(settings={}):
         df, image_paths_tmp = correct_paths(df, settings['src'][i])
         all_df = pd.concat([all_df, df], axis=0)
         #image_paths.extend(image_paths_tmp)
-
+        
     all_df['cond'] = all_df['column_name'].apply(map_condition, neg=settings['neg'], pos=settings['pos'], mix=settings['mix'])
 
     if settings['exclude_conditions']:
@@ -493,7 +505,7 @@ def generate_image_umap(settings={}):
         
         # Extract and reset the index for the column to compare
         col_to_compare = all_df[settings['col_to_compare']].reset_index(drop=True)
-        
+        print(col_to_compare)
         #if settings['only_top_features']:
         #    column_list = None
             
@@ -781,8 +793,10 @@ def generate_mediar_masks(src, settings, object_type):
     if not torch.cuda.is_available():
         print(f'Torch CUDA is not available, using CPU')
 
+    settings['src'] = src
+
     # Preprocess settings
-    settings = set_default_settings_preprocess_generate_masks(src, settings)
+    settings = set_default_settings_preprocess_generate_masks(settings)
 
     if settings['verbose']:
         settings_df = pd.DataFrame(list(settings.items()), columns=['setting_key', 'setting_value'])

spacr/deep_spacr.py

@@ -12,6 +12,7 @@ from PIL import Image
 from sklearn.metrics import auc, precision_recall_curve
 from IPython.display import display
 from multiprocessing import cpu_count
+import torch.optim as optim
 
 from torchvision import transforms
 from torch.utils.data import DataLoader
@@ -76,10 +77,11 @@ def apply_model_to_tar(settings={}):
     from .io import TarImageDataset
     from .utils import process_vision_results, print_progress
 
-    if os.path.exists(settings['dataset']):
-        tar_path = settings['dataset']
-    else:
-        tar_path = os.path.join(settings['src'], 'datasets', settings['dataset'])
+    #if os.path.exists(settings['dataset']):
+    #    tar_path = settings['dataset']
+    #else:
+    #    tar_path = os.path.join(settings['src'], 'datasets', settings['dataset'])
+    tar_path = settings['tar_path']
     model_path = settings['model_path']
     
     device = torch.device("cuda:0" if torch.cuda.is_available() else "cpu")
@@ -103,7 +105,8 @@ def apply_model_to_tar(settings={}):
     data_loader = DataLoader(dataset, batch_size=settings['batch_size'], shuffle=True, num_workers=settings['n_jobs'], pin_memory=True)
     
     model_name = os.path.splitext(os.path.basename(model_path))[0]
-    dataset_name = os.path.splitext(os.path.basename(settings['dataset']))[0]  
+    #dataset_name = os.path.splitext(os.path.basename(settings['dataset']))[0] 
+    dataset_name = os.path.splitext(os.path.basename(settings['tar_path']))[0] 
     date_name = datetime.date.today().strftime('%y%m%d')
     dst = os.path.dirname(tar_path)
     result_loc = f'{dst}/{date_name}_{dataset_name}_{model_name}_result.csv'
@@ -934,3 +937,373 @@ def deep_spacr(settings={}):
             
         if os.path.exists(settings['model_path']):
             apply_model_to_tar(settings)
+            
+def model_knowledge_transfer(
+    teacher_paths,
+    student_save_path,
+    data_loader,                 # A DataLoader with (images, labels)
+    device='cpu',
+    student_model_name='maxvit_t',
+    pretrained=True,
+    dropout_rate=None,
+    use_checkpoint=False,
+    alpha=0.5,
+    temperature=2.0,
+    lr=1e-4,
+    epochs=10
+):
+    """
+    Performs multi-teacher knowledge distillation on a new labeled dataset,
+    producing a single student TorchModel that combines (distills) the 
+    teachers' knowledge plus the labeled data.
+
+    Usage:
+        student = model_knowledge_transfer(
+            teacher_paths=[
+                'teacherA.pth', 
+                'teacherB.pth', 
+                ...
+            ],
+            student_save_path='distilled_student.pth',
+            data_loader=my_data_loader, 
+            device='cuda', 
+            student_model_name='maxvit_t',
+            alpha=0.5,
+            temperature=2.0,
+            lr=1e-4,
+            epochs=10
+        )
+
+    Then load it via:
+        fused_student = torch.load('distilled_student.pth')
+        # fused_student is a TorchModel instance, ready for inference.
+
+    Args:
+        teacher_paths (list[str]): List of paths to teacher models (TorchModel 
+            or dict with 'model' in it). They must have the same architecture 
+            or at least produce the same dimension of output.
+        student_save_path (str): Destination path to save the final student 
+            TorchModel. 
+        data_loader (DataLoader): Yields (images, labels) from the new dataset.
+        device (str): 'cpu' or 'cuda'.
+        student_model_name (str): Architecture name for the student TorchModel.
+        pretrained (bool): If the student should be initialized as pretrained.
+        dropout_rate (float): If needed by your TorchModel init.
+        use_checkpoint (bool): If needed by your TorchModel init.
+        alpha (float): Weight balancing real-label CE vs. distillation loss 
+            (0..1). 
+        temperature (float): Distillation temperature (>1 typically).
+        lr (float): Learning rate for the student.
+        epochs (int): Number of training epochs.
+
+    Returns:
+        TorchModel: The final, trained student model.
+    """
+    from spacr.utils import TorchModel  # Adapt if needed
+
+    # Adjust filename to reflect knowledge-distillation if desired
+    if student_save_path.endswith('.pth'):
+        base, ext = os.path.splitext(student_save_path)
+    else:
+        base = student_save_path
+    student_save_path = base + '_KD.pth'
+
+    # -- 1. Load teacher models --
+    teachers = []
+    print("Loading teacher models:")
+    for path in teacher_paths:
+        print(f"  Loading teacher: {path}")
+        ckpt = torch.load(path, map_location=device)
+        if isinstance(ckpt, TorchModel):
+            teacher = ckpt.to(device)
+        elif isinstance(ckpt, dict):
+            # If it's a dict with 'model' inside
+            # We might need to check if it has 'model_name', etc. 
+            # But let's keep it simple: same architecture as the student
+            teacher = TorchModel(
+                model_name=ckpt.get('model_name', student_model_name),
+                pretrained=ckpt.get('pretrained', pretrained),
+                dropout_rate=ckpt.get('dropout_rate', dropout_rate),
+                use_checkpoint=ckpt.get('use_checkpoint', use_checkpoint)
+            ).to(device)
+            teacher.load_state_dict(ckpt['model'])
+        else:
+            raise ValueError(f"Unsupported checkpoint type at {path} (must be TorchModel or dict).")
+
+        teacher.eval()  # For consistent batchnorm, dropout
+        teachers.append(teacher)
+
+    # -- 2. Initialize the student TorchModel --
+    student_model = TorchModel(
+        model_name=student_model_name,
+        pretrained=pretrained,
+        dropout_rate=dropout_rate,
+        use_checkpoint=use_checkpoint
+    ).to(device)
+
+    # You could load a partial checkpoint into the student here if desired.
+
+    # -- 3. Optimizer --
+    optimizer = optim.Adam(student_model.parameters(), lr=lr)
+
+    # Distillation training loop
+    for epoch in range(epochs):
+        student_model.train()
+        running_loss = 0.0
+
+        for images, labels in data_loader:
+            images, labels = images.to(device), labels.to(device)
+
+            # Forward pass student
+            logits_s = student_model(images)         # shape: (B, num_classes)
+            logits_s_temp = logits_s / temperature   # scale by T
+
+            # Distillation from teachers
+            with torch.no_grad():
+                # We'll average teacher probabilities
+                teacher_probs_list = []
+                for tm in teachers:
+                    logits_t = tm(images) / temperature
+                    # convert to probabilities
+                    teacher_probs_list.append(F.softmax(logits_t, dim=1))
+                # average them
+                teacher_probs_ensemble = torch.mean(torch.stack(teacher_probs_list), dim=0)
+
+            # Student probabilities (log-softmax)
+            student_log_probs = F.log_softmax(logits_s_temp, dim=1)
+
+            # Distillation loss => KLDiv
+            loss_distill = F.kl_div(
+                student_log_probs,
+                teacher_probs_ensemble,
+                reduction='batchmean'
+            ) * (temperature ** 2)
+
+            # Real label loss => cross-entropy
+            # We can compute this on the raw logits or scaled. Typically raw logits is standard:
+            loss_ce = F.cross_entropy(logits_s, labels)
+
+            # Weighted sum
+            loss = alpha * loss_ce + (1 - alpha) * loss_distill
+
+            optimizer.zero_grad()
+            loss.backward()
+            optimizer.step()
+
+            running_loss += loss.item()
+
+        avg_loss = running_loss / len(data_loader)
+        print(f"Epoch [{epoch+1}/{epochs}] - Loss: {avg_loss:.4f}")
+
+    # -- 4. Save final student as a TorchModel --
+    torch.save(student_model, student_save_path)
+    print(f"Knowledge-distilled student saved to: {student_save_path}")
+
+    return student_model
+            
+def model_fusion(model_paths,
+                 save_path,
+                 device='cpu',
+                 model_name='maxvit_t',
+                 pretrained=True,
+                 dropout_rate=None,
+                 use_checkpoint=False,
+                 aggregator='mean'):
+    """
+    Fuses an arbitrary number of TorchModel instances by combining their weights 
+    (using mean, geomean, median, sum, max, or min) and saves the entire fused 
+    model object. 
+
+    You can later load the fused model with:
+        model = torch.load('fused_model.pth')
+
+    which returns a ready-to-use TorchModel instance.
+
+    Parameters:
+        model_paths (list of str): Paths to the model checkpoints to fuse.
+                                   Each checkpoint can be:
+                                     - A dict with keys ['model', 'model_name', ...]
+                                     - A TorchModel instance
+        save_path (str): Destination path to save the fused model.
+        device (str): 'cpu' or 'cuda' for loading weights and final model device.
+        model_name (str): Default model name (used if not in checkpoint).
+        pretrained (bool): Default if not in checkpoint.
+        dropout_rate (float): Default if not in checkpoint.
+        use_checkpoint (bool): Default if not in checkpoint.
+        aggregator (str): How to combine weights across models:
+                            'mean', 'geomean', 'median', 'sum', 'max', or 'min'.
+
+    Returns:
+        fused_model (TorchModel): The final fused TorchModel instance 
+                                  with combined weights.
+    """
+    from spacr.utils import TorchModel
+    
+    if save_path.endswith('.pth'):
+        save_path_part1, ext = os.path.splitext(save_path)
+    else:
+        save_path_part1 = save_path
+    
+    save_path = save_path_part1 + f'_{aggregator}.pth'
+
+    valid_aggregators = {'mean', 'geomean', 'median', 'sum', 'max', 'min'}
+    if aggregator not in valid_aggregators:
+        raise ValueError(f"Invalid aggregator '{aggregator}'. "
+                         f"Must be one of {valid_aggregators}.")
+
+    # --- 1. Load the first checkpoint to figure out architecture & hyperparams ---
+    print(f"Loading the first model from: {model_paths[0]} to derive architecture")
+    first_ckpt = torch.load(model_paths[0], map_location=device)
+
+    if isinstance(first_ckpt, dict):
+        # It's a dict with state_dict + possibly metadata
+        # Use any stored metadata if present
+        model_name = first_ckpt.get('model_name', model_name)
+        pretrained = first_ckpt.get('pretrained', pretrained)
+        dropout_rate = first_ckpt.get('dropout_rate', dropout_rate)
+        use_checkpoint = first_ckpt.get('use_checkpoint', use_checkpoint)
+
+        # Initialize the fused model
+        fused_model = TorchModel(
+            model_name=model_name,
+            pretrained=pretrained,
+            dropout_rate=dropout_rate,
+            use_checkpoint=use_checkpoint
+        ).to(device)
+
+        # We'll collect state dicts in a list
+        state_dicts = [first_ckpt['model']]  # the actual weights
+    elif isinstance(first_ckpt, TorchModel):
+        # The checkpoint is directly a TorchModel instance
+        fused_model = first_ckpt.to(device)
+        state_dicts = [fused_model.state_dict()]
+    else:
+        raise ValueError("Unsupported checkpoint format. Must be a dict or a TorchModel instance.")
+
+    # --- 2. Load the rest of the checkpoints ---
+    for path in model_paths[1:]:
+        print(f"Loading model from: {path}")
+        ckpt = torch.load(path, map_location=device)
+        if isinstance(ckpt, dict):
+            state_dicts.append(ckpt['model'])  # Just the state dict portion
+        elif isinstance(ckpt, TorchModel):
+            state_dicts.append(ckpt.state_dict())
+        else:
+            raise ValueError(f"Unsupported checkpoint format in {path} (must be dict or TorchModel).")
+
+    # --- 3. Verify all state dicts have the same keys ---
+    fused_sd = fused_model.state_dict()
+    for sd in state_dicts:
+        if fused_sd.keys() != sd.keys():
+            raise ValueError("All models must have identical architecture/state_dict keys.")
+
+    # --- 4. Define aggregator logic ---
+    def combine_tensors(tensor_list, mode='mean'):
+        """Given a list of Tensors, combine them using the chosen aggregator."""
+        # stack along new dimension => shape (num_models, *tensor.shape)
+        stacked = torch.stack(tensor_list, dim=0).float()
+
+        if mode == 'mean':
+            return stacked.mean(dim=0)
+        elif mode == 'geomean':
+            # geometric mean = exp(mean(log(tensor))) 
+            # caution: requires all > 0
+            return torch.exp(torch.log(stacked).mean(dim=0))
+        elif mode == 'median':
+            return stacked.median(dim=0).values
+        elif mode == 'sum':
+            return stacked.sum(dim=0)
+        elif mode == 'max':
+            return stacked.max(dim=0).values
+        elif mode == 'min':
+            return stacked.min(dim=0).values
+        else:
+            raise ValueError(f"Unsupported aggregator: {mode}")
+
+    # --- 5. Combine the weights ---
+    for key in fused_sd.keys():
+        # gather all versions of this tensor
+        all_tensors = [sd[key] for sd in state_dicts]
+        fused_sd[key] = combine_tensors(all_tensors, mode=aggregator)
+
+    # Load combined weights into the fused model
+    fused_model.load_state_dict(fused_sd)
+
+    # --- 6. Save the entire TorchModel object ---
+    torch.save(fused_model, save_path)
+    print(f"Fused model (aggregator='{aggregator}') saved as a full TorchModel to: {save_path}")
+
+    return fused_model
+
+def annotate_filter_vision(settings):
+    
+    from .utils import annotate_conditions
+    
+    def filter_csv_by_png(csv_file):
+        """
+        Filters a DataFrame by removing rows that match PNG filenames in a folder.
+
+        Parameters:
+            csv_file (str): Path to the CSV file.
+
+        Returns:
+            pd.DataFrame: Filtered DataFrame.
+        """
+        # Split the path to identify the datasets folder and build the training folder path
+        before_datasets, after_datasets = csv_file.split(os.sep + "datasets" + os.sep, 1)
+        train_fldr = os.path.join(before_datasets, 'datasets', 'training', 'train')
+
+        # Paths for train/nc and train/pc
+        nc_folder = os.path.join(train_fldr, 'nc')
+        pc_folder = os.path.join(train_fldr, 'pc')
+
+        # Load the CSV file into a DataFrame
+        df = pd.read_csv(csv_file)
+
+        # Collect PNG filenames from train/nc and train/pc
+        png_files = set()
+        for folder in [nc_folder, pc_folder]:
+            if os.path.exists(folder):  # Ensure the folder exists
+                png_files.update({file for file in os.listdir(folder) if file.endswith(".png")})
+
+        # Filter the DataFrame by excluding rows where filenames match PNG files
+        filtered_df = df[~df['path'].isin(png_files)]
+
+        return filtered_df
+    
+    if isinstance(settings['src'], str):
+        settings['src'] = [settings['src']]
+    
+    for src in settings['src']:
+        ann_src, ext = os.path.splitext(src)
+        output_csv = ann_src+'_annotated_filtered.csv'
+        print(output_csv)
+
+        df = pd.read_csv(src)
+        
+        if 'column_name' not in df.columns:
+            df['column_name'] = df['column']
+            
+        df = annotate_conditions(df, 
+                            cells=settings['cells'],
+                            cell_loc=settings['cell_loc'],
+                            pathogens=settings['pathogens'],
+                            pathogen_loc=settings['pathogen_loc'],
+                            treatments=settings['treatments'],
+                            treatment_loc=settings['treatment_loc'])
+        
+        if not settings['filter_column'] is None:
+            if settings['filter_column'] in df.columns:
+                filtered_df = df[(df[settings['filter_column']] > settings['upper_threshold']) | (df[settings['filter_column']] < settings['lower_threshold'])]
+                print(f'Filtered DataFrame with {len(df)} rows to {len(filtered_df)} rows.')
+            else:
+                print(f"{settings['filter_column']} not in DataFrame columns.")
+                filtered_df = df
+        else:
+            filtered_df = df
+                
+        filtered_df.to_csv(output_csv, index=False)
+        
+        if settings['remove_train']:
+            df = filter_csv_by_png(output_csv)
+            df.to_csv(output_csv, index=False)

spacr/gui_core.py

@@ -324,40 +324,6 @@ def show_next_figure():
         index_control.set(figure_index)
         index_control.set_to(len(figures) - 1)
         display_figure(fig)
-
-def process_fig_queue_v1():
-    global canvas, fig_queue, canvas_widget, parent_frame, uppdate_frequency, figures, figure_index, index_control
-    from .gui_elements import standardize_figure
-
-    #print("process_fig_queue called", flush=True)
-    try:
-        while not fig_queue.empty():
-            fig = fig_queue.get_nowait()
-            if fig is None:
-                print("Warning: Retrieved a None figure from fig_queue.", flush=True)
-                continue
-
-            # Standardize the figure appearance before adding it
-            fig = standardize_figure(fig)
-            figures.append(fig)
-
-            # Update slider maximum
-            index_control.set_to(len(figures) - 1)
-
-            # If no figure has been displayed yet
-            if figure_index == -1:
-                figure_index = 0
-                display_figure(figures[figure_index])
-                index_control.set(figure_index)
-
-    except Exception as e:
-        print("Exception in process_fig_queue:", e, flush=True)
-        traceback.print_exc()
-
-    finally:
-        # Schedule process_fig_queue() to run again
-        after_id = canvas_widget.after(uppdate_frequency, process_fig_queue)
-        parent_frame.after_tasks.append(after_id)
         
 def process_fig_queue():
     global canvas, fig_queue, canvas_widget, parent_frame, uppdate_frequency, figures, figure_index, index_control
@@ -544,7 +510,7 @@ def import_settings(settings_type='mask'):
     #vars_dict = hide_all_settings(vars_dict, categories=None)
     csv_settings = read_settings_from_csv(csv_file_path)
     if settings_type == 'mask':
-        settings = set_default_settings_preprocess_generate_masks(src='path', settings={})
+        settings = set_default_settings_preprocess_generate_masks(settings={})
     elif settings_type == 'measure':
         settings = get_measure_crop_settings(settings={})
     elif settings_type == 'classify':
@@ -596,7 +562,7 @@ def setup_settings_panel(vertical_container, settings_type='mask'):
     settings_frame.grid_columnconfigure(0, weight=1)
 
     if settings_type == 'mask':
-        settings = set_default_settings_preprocess_generate_masks(src='path', settings={})
+        settings = set_default_settings_preprocess_generate_masks(settings={})
     elif settings_type == 'measure':
         settings = get_measure_crop_settings(settings={})
     elif settings_type == 'classify':
@@ -912,7 +878,7 @@ def start_process(q=None, fig_queue=None, settings_type='mask'):
         q.put(f"Error: {e}")
         return
 
-    if thread_control.get("run_thread") is not None:
+    if isinstance(thread_control, dict) and thread_control.get("run_thread") is not None:
         initiate_abort()
     
     stop_requested = Value('i', 0)
@@ -1018,6 +984,66 @@ def main_thread_update_function(root, q, fig_queue, canvas_widget):
         print(f"Error updating GUI canvas: {e}")
     finally:
         root.after(uppdate_frequency, lambda: main_thread_update_function(root, q, fig_queue, canvas_widget))
+        
+def cleanup_previous_instance():
+    """
+    Cleans up resources from the previous application instance.
+    """
+    global parent_frame, usage_bars, figures, figure_index, thread_control, canvas, q, fig_queue
+
+    # 1. Destroy all widgets in the parent frame
+    if parent_frame is not None:
+        for widget in parent_frame.winfo_children():
+            try:
+                widget.destroy()
+            except Exception as e:
+                print(f"Error destroying widget: {e}")
+        parent_frame.update_idletasks()
+        parent_frame = None
+
+    # 2. Cancel all pending `after` tasks
+    if parent_frame is not None:
+        parent_window = parent_frame.winfo_toplevel()
+        if hasattr(parent_window, 'after_tasks'):
+            for after_id in parent_window.after_tasks:
+                parent_window.after_cancel(after_id)
+            parent_window.after_tasks = []
+
+    # 3. Clear global queues
+    if q is not None:
+        while not q.empty():
+            q.get()
+        q = None
+
+    if fig_queue is not None:
+        while not fig_queue.empty():
+            fig_queue.get()
+        fig_queue = None
+
+    # 4. Stop and reset global thread control
+    if thread_control is not None:
+        thread_control['stop'] = True
+        #thread_control = None
+
+    # 5. Reset usage bars, figures, and indices
+    usage_bars = []
+    figures = deque()
+    figure_index = -1
+
+    # 6. Clear canvas or other visualizations
+    if canvas is not None:
+        try:
+            if hasattr(canvas, 'figure'):  # Check if it's a FigureCanvasTkAgg
+                canvas.figure.clear()  # Clear the Matplotlib figure
+                canvas.get_tk_widget().destroy()  # Destroy the Tkinter widget
+            else:
+                # Assume it's a standard Tkinter Canvas
+                canvas.delete("all")
+        except Exception as e:
+            print(f"Error clearing canvas: {e}")
+        canvas = None
+
+    print("Previous instance cleaned up successfully.")
 
 def initiate_root(parent, settings_type='mask'):
     """
@@ -1033,7 +1059,11 @@ def initiate_root(parent, settings_type='mask'):
     
     global q, fig_queue, thread_control, parent_frame, scrollable_frame, button_frame, vars_dict, canvas, canvas_widget, button_scrollable_frame, progress_bar, uppdate_frequency, figures, figure_index, index_control, usage_bars
     
-    from .gui_utils import setup_frame, get_screen_dimensions
+    # Clean up any previous instance
+    cleanup_previous_instance()
+    
+    from .gui_utils import setup_frame
+    from .gui_elements import create_menu_bar
     from .settings import descriptions
     #from .openai import Chatbot
 
@@ -1096,6 +1126,7 @@ def initiate_root(parent, settings_type='mask'):
         
         process_console_queue()
         process_fig_queue()
+        create_menu_bar(parent)
         after_id = parent_window.after(uppdate_frequency, lambda: main_thread_update_function(parent_window, q, fig_queue, canvas_widget))
         parent_window.after_tasks.append(after_id)