spacr 0.3.72__py3-none-any.whl → 0.3.81__py3-none-any.whl

spacr/gui_core.py

@@ -4,6 +4,7 @@ from tkinter import ttk
 from tkinter import filedialog
 from multiprocessing import Process, Value, Queue, set_start_method
 from tkinter import ttk
+import matplotlib
 from matplotlib.figure import Figure
 from matplotlib.backends.backend_tkagg import FigureCanvasTkAgg
 import numpy as np
@@ -169,7 +170,7 @@ def display_figure(fig):
             #flash_feedback("right")
             show_next_figure()
 
-    def zoom(event):
+    def zoom_v1(event):
         nonlocal scale_factor
 
         zoom_speed = 0.1  # Adjust the zoom speed for smoother experience
@@ -197,6 +198,70 @@ def display_figure(fig):
 
         # Redraw the figure efficiently
         canvas.draw_idle()
+        
+    def zoom_test(event):
+        if event.num == 4:  # Scroll up
+            print("zoom in")
+        elif event.num == 5: # Scroll down
+            print("zoom out")
+            
+    def zoom_2(event):
+        zoom_speed = 0.1  # Change this to control how fast you zoom
+
+        # Determine the zoom direction based on the scroll event
+        if event.num == 4 or (hasattr(event, 'delta') and event.delta > 0):  # Scroll up = zoom in
+            factor = 1 - zoom_speed
+        elif event.num == 5 or (hasattr(event, 'delta') and event.delta < 0): # Scroll down = zoom out
+            factor = 1 + zoom_speed
+        else:
+            return  # No recognized scroll direction
+
+        for ax in canvas.figure.get_axes():
+            xlim = ax.get_xlim()
+            ylim = ax.get_ylim()
+
+            x_center = (xlim[1] + xlim[0]) / 2
+            y_center = (ylim[1] + ylim[0]) / 2
+
+            x_range = (xlim[1] - xlim[0]) * factor
+            y_range = (ylim[1] - ylim[0]) * factor
+
+            # Set the new limits
+            ax.set_xlim([x_center - x_range / 2, x_center + x_range / 2])
+            ax.set_ylim([y_center - y_range / 2, y_center + y_range / 2])
+
+        # Redraw the figure efficiently
+        canvas.draw_idle()
+        
+    def zoom(event):
+        # Fixed zoom factors (adjust these if you want faster or slower zoom)
+        zoom_in_factor = 0.9   # When zooming in, ranges shrink by 10%
+        zoom_out_factor = 1.1  # When zooming out, ranges increase by 10%
+
+        # Determine the zoom direction based on the scroll event
+        if event.num == 4 or (hasattr(event, 'delta') and event.delta > 0):  # Scroll up = zoom in
+            factor = zoom_in_factor
+        elif event.num == 5 or (hasattr(event, 'delta') and event.delta < 0): # Scroll down = zoom out
+            factor = zoom_out_factor
+        else:
+            return  # No recognized scroll direction
+
+        for ax in canvas.figure.get_axes():
+            xlim = ax.get_xlim()
+            ylim = ax.get_ylim()
+
+            x_center = (xlim[1] + xlim[0]) / 2
+            y_center = (ylim[1] + ylim[0]) / 2
+
+            x_range = (xlim[1] - xlim[0]) * factor
+            y_range = (ylim[1] - ylim[0]) * factor
+
+            # Set the new limits
+            ax.set_xlim([x_center - x_range / 2, x_center + x_range / 2])
+            ax.set_ylim([y_center - y_range / 2, y_center + y_range / 2])
+
+        # Redraw the figure efficiently
+        canvas.draw_idle()
 
 
     # Bind events for hover, click interactions, and zoom
@@ -205,19 +270,20 @@ def display_figure(fig):
     canvas_widget.bind("<Button-1>", on_click)
     canvas_widget.bind("<Button-3>", on_right_click)
 
-
     # Detect the operating system and bind the appropriate mouse wheel events
     current_os = platform.system()
 
     if current_os == "Windows":
         canvas_widget.bind("<MouseWheel>", zoom)  # Windows
-    elif current_os == "Darwin":  # macOS
+    elif current_os == "Darwin":
         canvas_widget.bind("<MouseWheel>", zoom)
         canvas_widget.bind("<Button-4>", zoom)  # Scroll up
         canvas_widget.bind("<Button-5>", zoom)  # Scroll down
     elif current_os == "Linux":
         canvas_widget.bind("<Button-4>", zoom)  # Linux Scroll up
         canvas_widget.bind("<Button-5>", zoom)  # Linux Scroll down
+        
+    process_fig_queue()
 
 def clear_unused_figures():
     global figures, figure_index
@@ -230,71 +296,127 @@ def clear_unused_figures():
     figure_index = min(max(figure_index, 0), len(figures) - 1)
 
 def show_previous_figure():
-    global figure_index, figures, fig_queue
+    from .gui_elements import standardize_figure
+    global figure_index, figures, fig_queue, index_control
     
     if figure_index is not None and figure_index > 0:
         figure_index -= 1
+        index_control.set(figure_index)
+        figures[figure_index] = standardize_figure(figures[figure_index])
         display_figure(figures[figure_index])
-        clear_unused_figures()
+        #clear_unused_figures()
 
 def show_next_figure():
-    global figure_index, figures, fig_queue
+    from .gui_elements import standardize_figure
+    global figure_index, figures, fig_queue, index_control
     if figure_index is not None and figure_index < len(figures) - 1:
         figure_index += 1
+        index_control.set(figure_index)
+        index_control.set_to(len(figures) - 1)
+        figures[figure_index] = standardize_figure(figures[figure_index])
         display_figure(figures[figure_index])
-        clear_unused_figures()
+        #clear_unused_figures()
+        
     elif figure_index == len(figures) - 1 and not fig_queue.empty():
         fig = fig_queue.get_nowait()
         figures.append(fig)
         figure_index += 1
+        index_control.set(figure_index)
+        index_control.set_to(len(figures) - 1)
         display_figure(fig)
-                       
-def process_fig_queue():
-    global canvas, fig_queue, canvas_widget, parent_frame, uppdate_frequency, figures, figure_index, index_control
 
+def process_fig_queue_v1():
+    global canvas, fig_queue, canvas_widget, parent_frame, uppdate_frequency, figures, figure_index, index_control
     from .gui_elements import standardize_figure
+
+    #print("process_fig_queue called", flush=True)
     try:
         while not fig_queue.empty():
             fig = fig_queue.get_nowait()
+            if fig is None:
+                print("Warning: Retrieved a None figure from fig_queue.", flush=True)
+                continue
+
+            # Standardize the figure appearance before adding it
+            fig = standardize_figure(fig)
+            figures.append(fig)
+
+            # Update slider maximum
+            index_control.set_to(len(figures) - 1)
 
+            # If no figure has been displayed yet
+            if figure_index == -1:
+                figure_index = 0
+                display_figure(figures[figure_index])
+                index_control.set(figure_index)
+
+    except Exception as e:
+        print("Exception in process_fig_queue:", e, flush=True)
+        traceback.print_exc()
+
+    finally:
+        # Schedule process_fig_queue() to run again
+        after_id = canvas_widget.after(uppdate_frequency, process_fig_queue)
+        parent_frame.after_tasks.append(after_id)
+        
+def process_fig_queue():
+    global canvas, fig_queue, canvas_widget, parent_frame, uppdate_frequency, figures, figure_index, index_control
+    from .gui_elements import standardize_figure
+
+    try:
+        while not fig_queue.empty():
+            fig = fig_queue.get_nowait()
             if fig is None:
                 print("Warning: Retrieved a None figure from fig_queue.")
-                continue  # Skip processing if the figure is None
+                continue
 
-            # Standardize the figure appearance before adding it to the list
+            # Standardize the figure appearance before adding it
             fig = standardize_figure(fig)
-
             figures.append(fig)
 
-            # Update the slider range and set the value to the latest figure index
+            # OPTIONAL: Cap the size of the figures deque at 100
+            MAX_FIGURES = 100
+            while len(figures) > MAX_FIGURES:
+                # Discard the oldest figure
+                old_fig = figures.popleft()
+                # If needed, you could also close the figure to free memory:
+                matplotlib.pyplot.close(old_fig)
+
+            # Update slider maximum
             index_control.set_to(len(figures) - 1)
 
+            # If no figure has been displayed yet
             if figure_index == -1:
-                figure_index += 1
+                figure_index = 0
                 display_figure(figures[figure_index])
                 index_control.set(figure_index)
-            
+
     except Exception as e:
+        print("Exception in process_fig_queue:", e)
         traceback.print_exc()
+
     finally:
+        # Schedule process_fig_queue() to run again
         after_id = canvas_widget.after(uppdate_frequency, process_fig_queue)
         parent_frame.after_tasks.append(after_id)
 
-def update_figure(value):
-    global figure_index, figures
 
+def update_figure(value):
+    from .gui_elements import standardize_figure
+    global figure_index, figures, index_control
+    
     # Convert the value to an integer
     index = int(value)
     
     # Check if the index is valid
     if 0 <= index < len(figures):
         figure_index = index
+        figures[figure_index] = standardize_figure(figures[figure_index])
         display_figure(figures[figure_index])
-
-    # Update the index control widget's range and value
-    index_control.set_to(len(figures) - 1)
-    index_control.set(figure_index)
-
+        index_control.set(figure_index)
+        print("update_figure called with value:", figure_index)
+        index_control.set_to(len(figures) - 1)
+        
 def setup_plot_section(vertical_container, settings_type):
     global canvas, canvas_widget, figures, figure_index, index_control
     from .gui_utils import display_media_in_plot_frame
@@ -305,29 +427,29 @@ def setup_plot_section(vertical_container, settings_type):
 
     # Initialize deque for storing figures and the current index
     figures = deque()
+    figure_index = -1  # Start with no figure displayed
 
     # Create a frame for the plot section
     plot_frame = tk.Frame(vertical_container)
     plot_frame.configure(bg=bg)
     vertical_container.add(plot_frame, stretch="always")
 
-    # Clear the plot_frame (optional, to handle cases where it may already have content)
+    # Clear the plot_frame (optional)
     for widget in plot_frame.winfo_children():
         widget.destroy()
 
-    # Create a figure and plot
+    # Create a figure and plot (initial figure)
     figure = Figure(figsize=(30, 4), dpi=100)
     plot = figure.add_subplot(111)
     plot.plot([], [])
     plot.axis('off')
 
     if settings_type == 'map_barcodes':
-        # Load and display GIF
         current_dir = os.path.dirname(__file__)
         resources_path = os.path.join(current_dir, 'resources', 'icons')
         gif_path = os.path.join(resources_path, 'dna_matrix.mp4')
-
         display_media_in_plot_frame(gif_path, plot_frame)
+
         canvas = FigureCanvasTkAgg(figure, master=plot_frame)
         canvas.get_tk_widget().configure(cursor='arrow', highlightthickness=0)
         canvas_widget = canvas.get_tk_widget()
@@ -348,10 +470,11 @@ def setup_plot_section(vertical_container, settings_type):
     # Create slider
     control_frame = tk.Frame(plot_frame, height=15*2,  bg=bg)
     control_frame.grid(row=1, column=0, sticky="ew", padx=10, pady=5)
-    control_frame.grid_propagate(False) 
+    control_frame.grid_propagate(False)
 
-    # Pass the update_figure function as the command to spacrSlider
-    index_control = spacrSlider(control_frame, from_=0, to=0, value=0, thickness=2, knob_radius=10, position="center", show_index=True, command=update_figure)
+    index_control = spacrSlider(control_frame, from_=0, to=0, value=0, thickness=2, knob_radius=10,
+                                position="center", show_index=True, command=update_figure)
+    
     index_control.grid(row=0, column=0, sticky="ew")
     control_frame.grid_columnconfigure(0, weight=1)
 
@@ -359,10 +482,17 @@ def setup_plot_section(vertical_container, settings_type):
     style = ttk.Style(vertical_container)
     _ = set_dark_style(style, containers=containers, widgets=widgets)
 
+    # Now ensure the first figure is displayed and recognized:
+    figures.append(figure)
+    figure_index = 0
+    display_figure(figures[figure_index])
+    index_control.set_to(len(figures) - 1)   # Slider max = 0 in this case, since there's only one figure
+    index_control.set(figure_index)          # Set slider to 0 to indicate the first figure
+
     return canvas, canvas_widget
 
-def set_globals(thread_control_var, q_var, console_output_var, parent_frame_var, vars_dict_var, canvas_var, canvas_widget_var, scrollable_frame_var, fig_queue_var, figures_var, figure_index_var, index_control_var, progress_bar_var, usage_bars_var):
-    global thread_control, q, console_output, parent_frame, vars_dict, canvas, canvas_widget, scrollable_frame, fig_queue, figures, figure_index, progress_bar, usage_bars, index_control
+def set_globals(thread_control_var, q_var, console_output_var, parent_frame_var, vars_dict_var, canvas_var, canvas_widget_var, scrollable_frame_var, fig_queue_var, progress_bar_var, usage_bars_var):
+    global thread_control, q, console_output, parent_frame, vars_dict, canvas, canvas_widget, scrollable_frame, fig_queue, progress_bar, usage_bars
     thread_control = thread_control_var
     q = q_var
     console_output = console_output_var
@@ -372,11 +502,11 @@ def set_globals(thread_control_var, q_var, console_output_var, parent_frame_var,
     canvas_widget = canvas_widget_var
     scrollable_frame = scrollable_frame_var
     fig_queue = fig_queue_var
-    figures = figures_var
-    figure_index = figure_index_var
+    #figures = figures_var
+    #figure_index = figure_index_var
+    #index_control = index_control_var
     progress_bar = progress_bar_var
     usage_bars = usage_bars_var
-    index_control = index_control_var
 
 def import_settings(settings_type='mask'):
     global vars_dict, scrollable_frame, button_scrollable_frame
@@ -606,6 +736,7 @@ def setup_button_section(horizontal_container, settings_type='mask', run=True, a
         widgets.append(import_button)
         btn_row += 1
 
+    btn_row += 1
     # Add the batch progress bar
     progress_bar = spacrProgressBar(button_scrollable_frame.scrollable_frame, orient='horizontal', mode='determinate')
     progress_bar.grid(row=btn_row, column=0, columnspan=7, pady=5, padx=5, sticky='ew')
@@ -853,7 +984,8 @@ def process_console_queue():
                     if progress_bar:
                         progress_bar['maximum'] = total_progress
                         progress_bar['value'] = unique_progress_count
-
+                        #print("Current progress bar value:", progress_bar['value']) # Debugg
+                        
                     # Store operation type and additional info
                     if operation_type:
                         progress_bar.operation_type = operation_type
@@ -955,7 +1087,7 @@ def initiate_root(parent, settings_type='mask'):
         else:
             usage_bars = []
 
-        set_globals(thread_control, q, console_output, parent_frame, vars_dict, canvas, canvas_widget, scrollable_frame, fig_queue, figures, figure_index, index_control, progress_bar, usage_bars)
+        set_globals(thread_control, q, console_output, parent_frame, vars_dict, canvas, canvas_widget, scrollable_frame, fig_queue, progress_bar, usage_bars)
         description_text = descriptions.get(settings_type, "No description available for this module.")
         
         q.put(f"Console")

spacr/gui_elements.py

@@ -17,8 +17,7 @@ from skimage.draw import polygon, line
 from skimage.transform import resize
 from scipy.ndimage import binary_fill_holes, label
 from tkinter import ttk, scrolledtext
-from skimage.color import rgb2gray 
-
+from skimage.color import rgb2gray
 fig = None
 
 def create_menu_bar(root):
@@ -667,7 +666,7 @@ class spacrProgressBar(ttk.Progressbar):
         # Remove any borders and ensure the active color fills the entire space
         self.style.configure(
             "spacr.Horizontal.TProgressbar",
-            troughcolor=self.inactive_color,       # Set the trough to bg color
+            troughcolor=self.inactive_color, # Set the trough to bg color
             background=self.active_color,    # Active part is the active color
             borderwidth=0,                   # Remove border width
             pbarrelief="flat",               # Flat relief for the progress bar

spacr/ml.py

@@ -27,6 +27,9 @@ from sklearn.linear_model import Lasso, Ridge
 from sklearn.preprocessing import FunctionTransformer
 from patsy import dmatrices
 
+from sklearn.metrics import classification_report, accuracy_score
+from sklearn.model_selection import StratifiedKFold, cross_val_score
+from sklearn.feature_selection import SelectKBest, f_classif
 from sklearn.model_selection import train_test_split
 from sklearn.ensemble import RandomForestClassifier, HistGradientBoostingClassifier
 from sklearn.linear_model import LogisticRegression
@@ -1165,21 +1168,29 @@ def generate_ml_scores(settings):
 
     settings = set_default_analyze_screen(settings)
 
-    src = settings['src']
+    srcs = settings['src']
 
     settings_df = pd.DataFrame(list(settings.items()), columns=['Key', 'Value'])
     display(settings_df)
-
-    db_loc = [src+'/measurements/measurements.db']
-    tables = ['cell', 'nucleus', 'pathogen','cytoplasm']
-
-    nuclei_limit, pathogen_limit = settings['nuclei_limit'], settings['pathogen_limit']
     
-    df, _ = _read_and_merge_data(db_loc, 
-                                 tables,
-                                 settings['verbose'],
-                                 nuclei_limit,
-                                 pathogen_limit)
+    if isinstance(srcs, str):
+        srcs = [srcs]
+    
+    df = pd.DataFrame()
+    for idx, src in enumerate(srcs):
+        
+        if idx == 0:
+            src1 = src
+
+        db_loc = [src+'/measurements/measurements.db']
+        tables = ['cell', 'nucleus', 'pathogen','cytoplasm']
+        
+        dft, _ = _read_and_merge_data(db_loc, 
+                                    tables,
+                                    settings['verbose'],
+                                    nuclei_limit=settings['nuclei_limit'],
+                                    pathogen_limit=settings['pathogen_limit'])
+        df = pd.concat([df, dft])
     
     if settings['annotation_column'] is not None:
 
@@ -1191,6 +1202,7 @@ def generate_ml_scores(settings):
         annotated_df = png_list_df[['prcfo', settings['annotation_column']]].set_index('prcfo')
         df = annotated_df.merge(df, left_index=True, right_index=True)
         unique_values = df[settings['annotation_column']].dropna().unique()
+        
         if len(unique_values) == 1:
             unannotated_rows = df[df[settings['annotation_column']].isna()].index
             existing_value = unique_values[0]
@@ -1213,8 +1225,8 @@ def generate_ml_scores(settings):
             df[settings['annotation_column']] = df[settings['annotation_column']].apply(str)
     
     if settings['channel_of_interest'] in [0,1,2,3]:
-
-        df['recruitment'] = df[f"pathogen_channel_{settings['channel_of_interest']}_mean_intensity"]/df[f"cytoplasm_channel_{settings['channel_of_interest']}_mean_intensity"]
+        if f"pathogen_channel_{settings['channel_of_interest']}_mean_intensity" and f"cytoplasm_channel_{settings['channel_of_interest']}_mean_intensity" in df.columns:
+            df['recruitment'] = df[f"pathogen_channel_{settings['channel_of_interest']}_mean_intensity"]/df[f"cytoplasm_channel_{settings['channel_of_interest']}_mean_intensity"]
     
     output, figs = ml_analysis(df,
                                settings['channel_of_interest'],
@@ -1224,18 +1236,24 @@ def generate_ml_scores(settings):
                                settings['exclude'],
                                settings['n_repeats'],
                                settings['top_features'],
+                               settings['reg_alpha'],
+                               settings['reg_lambda'],
+                               settings['learning_rate'],                               
                                settings['n_estimators'],
                                settings['test_size'],
                                settings['model_type_ml'],
                                settings['n_jobs'],
                                settings['remove_low_variance_features'],
                                settings['remove_highly_correlated_features'],
+                               settings['prune_features'],
+                               settings['cross_validation'],
                                settings['verbose'])
     
     shap_fig = shap_analysis(output[3], output[4], output[5])
 
     features = output[0].select_dtypes(include=[np.number]).columns.tolist()
-
+    train_features_df = pd.DataFrame(output[9], columns=['feature'])
+    
     if not settings['heatmap_feature'] in features:
         raise ValueError(f"Variable {settings['heatmap_feature']} not found in the dataframe. Please choose one of the following: {features}")
     
@@ -1247,15 +1265,16 @@ def generate_ml_scores(settings):
                                 min_count=settings['minimum_cell_count'],
                                 verbose=settings['verbose'])
 
-    data_path, permutation_path, feature_importance_path, model_metricks_path, permutation_fig_path, feature_importance_fig_path, shap_fig_path, plate_heatmap_path, settings_csv = get_ml_results_paths(src, settings['model_type_ml'], settings['channel_of_interest'])
-    df, permutation_df, feature_importance_df, _, _, _, _, _, metrics_df = output
+    data_path, permutation_path, feature_importance_path, model_metricks_path, permutation_fig_path, feature_importance_fig_path, shap_fig_path, plate_heatmap_path, settings_csv, ml_features = get_ml_results_paths(src1, settings['model_type_ml'], settings['channel_of_interest'])
+    df, permutation_df, feature_importance_df, _, _, _, _, _, metrics_df, _ = output
 
     settings_df.to_csv(settings_csv, index=False)
     df.to_csv(data_path, mode='w', encoding='utf-8')
     permutation_df.to_csv(permutation_path, mode='w', encoding='utf-8')
     feature_importance_df.to_csv(feature_importance_path, mode='w', encoding='utf-8')
+    train_features_df.to_csv(ml_features, mode='w', encoding='utf-8')
     metrics_df.to_csv(model_metricks_path, mode='w', encoding='utf-8')
-    
+
     plate_heatmap.savefig(plate_heatmap_path, format='pdf')
     figs[0].savefig(permutation_fig_path, format='pdf')
     figs[1].savefig(feature_importance_fig_path, format='pdf')
@@ -1263,7 +1282,7 @@ def generate_ml_scores(settings):
 
     if settings['save_to_db']:
         settings['csv_path'] = data_path
-        settings['db_path'] = os.path.join(src, 'measurements', 'measurements.db')
+        settings['db_path'] = os.path.join(src1, 'measurements', 'measurements.db')
         settings['table_name'] = 'png_list'
         settings['update_column'] = 'predictions'
         settings['match_column'] = 'prcfo'
@@ -1271,7 +1290,7 @@ def generate_ml_scores(settings):
 
     return [output, plate_heatmap]
 
-def ml_analysis(df, channel_of_interest=3, location_column='column_name', positive_control='c2', negative_control='c1', exclude=None, n_repeats=10, top_features=30, n_estimators=100, test_size=0.2, model_type='xgboost', n_jobs=-1, remove_low_variance_features=True, remove_highly_correlated_features=True, verbose=False):
+def ml_analysis(df, channel_of_interest=3, location_column='column_name', positive_control='c2', negative_control='c1', exclude=None, n_repeats=10, top_features=30, reg_alpha=0.1, reg_lambda=1.0, learning_rate=0.00001, n_estimators=1000, test_size=0.2, model_type='xgboost', n_jobs=-1, remove_low_variance_features=True, remove_highly_correlated_features=True, prune_features=False, cross_validation=False, verbose=False):
     
     """
     Calculates permutation importance for numerical features in the dataframe,
@@ -1313,7 +1332,8 @@ def ml_analysis(df, channel_of_interest=3, location_column='column_name', positi
     if verbose:
         print(f'Found {len(features)} numerical features in the dataframe')
         print(f'Features used in training: {features}')
-
+        print(f'Features: {features}')
+        
     df = pd.concat([df, df_metadata[location_column]], axis=1)
 
     # Subset the dataframe based on specified column values
@@ -1327,14 +1347,26 @@ def ml_analysis(df, channel_of_interest=3, location_column='column_name', positi
     # Combine the subsets for analysis
     combined_df = pd.concat([df1, df2])
     combined_df = combined_df.drop(columns=[location_column])
+    
     if verbose:
         print(f'Found {len(df1)} samples for {negative_control} and {len(df2)} samples for {positive_control}. Total: {len(combined_df)}')
-
+    
     X = combined_df[features]
     y = combined_df['target']
-
-    print(X)
-    print(y)
+    
+    if prune_features:
+        before_pruning = len(X.columns)
+        selector = SelectKBest(score_func=f_classif, k=top_features)
+        X_selected = selector.fit_transform(X, y)
+        
+        # Get the selected feature names
+        selected_features = X.columns[selector.get_support()]
+        X = pd.DataFrame(X_selected, columns=selected_features, index=X.index)
+        
+        features = selected_features.tolist()
+        
+        after_pruning = len(X.columns)
+        print(f"Removed {before_pruning - after_pruning} features using SelectKBest")
 
     # Split the data into training and testing sets
     X_train, X_test, y_train, y_test = train_test_split(X, y, test_size=test_size, random_state=random_state)
@@ -1353,12 +1385,102 @@ def ml_analysis(df, channel_of_interest=3, location_column='column_name', positi
     elif model_type == 'gradient_boosting':
         model = HistGradientBoostingClassifier(max_iter=n_estimators, random_state=random_state)  # Supports n_jobs internally
     elif model_type == 'xgboost':
-        model = XGBClassifier(n_estimators=n_estimators, random_state=random_state, nthread=n_jobs, use_label_encoder=False, eval_metric='logloss')
+        model = XGBClassifier(reg_alpha=reg_alpha, reg_lambda=reg_lambda, learning_rate=learning_rate, n_estimators=n_estimators, random_state=random_state, nthread=n_jobs, use_label_encoder=False, eval_metric='logloss')
+        
     else:
         raise ValueError(f"Unsupported model_type: {model_type}")
 
-    model.fit(X_train, y_train)
+    # Perform k-fold cross-validation
+    if cross_validation:
+        
+        # Cross-validation setup
+        kfold = StratifiedKFold(n_splits=5, shuffle=True, random_state=random_state)
+        fold_metrics = []
+
+        for fold_idx, (train_index, test_index) in enumerate(kfold.split(X, y), start=1):
+            X_train, X_test = X.iloc[train_index], X.iloc[test_index]
+            y_train, y_test = y.iloc[train_index], y.iloc[test_index]
+
+            # Train the model
+            model.fit(X_train, y_train)
+
+            # Predict for the current test set
+            predictions_test = model.predict(X_test)
+            combined_df.loc[X_test.index, 'predictions'] = predictions_test
+
+            # Get prediction probabilities for the test set
+            prediction_probabilities_test = model.predict_proba(X_test)
 
+            # Find the optimal threshold
+            optimal_threshold = find_optimal_threshold(y_test, prediction_probabilities_test[:, 1])
+            if verbose:
+                print(f'Fold {fold_idx} - Optimal threshold: {optimal_threshold}')
+
+            # Assign predictions and probabilities to the test set in the DataFrame
+            df.loc[X_test.index, 'predictions'] = predictions_test
+            for i in range(prediction_probabilities_test.shape[1]):
+                df.loc[X_test.index, f'prediction_probability_class_{i}'] = prediction_probabilities_test[:, i]
+
+            # Evaluate performance for the current fold
+            fold_report = classification_report(y_test, predictions_test, output_dict=True)
+            fold_metrics.append(pd.DataFrame(fold_report).transpose())
+
+            if verbose:
+                print(f"Fold {fold_idx} Classification Report:")
+                print(classification_report(y_test, predictions_test))
+
+        # Aggregate metrics across all folds
+        metrics_df = pd.concat(fold_metrics).groupby(level=0).mean()
+
+        # Re-train on full data (X, y) and then apply to entire df
+        model.fit(X, y)  
+        all_predictions = model.predict(df[features])  # Predict on entire df
+        df['predictions'] = all_predictions
+
+        # Get prediction probabilities for all rows in df
+        prediction_probabilities = model.predict_proba(df[features])
+        for i in range(prediction_probabilities.shape[1]):
+            df[f'prediction_probability_class_{i}'] = prediction_probabilities[:, i]
+
+        if verbose:
+            print("\nFinal Classification Report on Full Dataset:")
+            print(classification_report(y, all_predictions))
+
+        # Generate metrics DataFrame
+        final_report_dict = classification_report(y, all_predictions, output_dict=True)
+        metrics_df = pd.DataFrame(final_report_dict).transpose()
+    
+    else:
+        model.fit(X_train, y_train)
+        # Predicting the target variable for the test set
+        predictions_test = model.predict(X_test)
+        combined_df.loc[X_test.index, 'predictions'] = predictions_test
+
+        # Get prediction probabilities for the test set
+        prediction_probabilities_test = model.predict_proba(X_test)
+
+        # Find the optimal threshold
+        optimal_threshold = find_optimal_threshold(y_test, prediction_probabilities_test[:, 1])
+        if verbose:
+            print(f'Optimal threshold: {optimal_threshold}')
+
+        # Predicting the target variable for all other rows in the dataframe
+        X_all = df[features]
+        all_predictions = model.predict(X_all)
+        df['predictions'] = all_predictions
+
+        # Get prediction probabilities for all rows in the dataframe
+        prediction_probabilities = model.predict_proba(X_all)
+        for i in range(prediction_probabilities.shape[1]):
+            df[f'prediction_probability_class_{i}'] = prediction_probabilities[:, i]
+            
+        if verbose:
+            print("\nClassification Report:")
+            print(classification_report(y_test, predictions_test))
+            
+        report_dict = classification_report(y_test, predictions_test, output_dict=True)
+        metrics_df = pd.DataFrame(report_dict).transpose()
+        
     perm_importance = permutation_importance(model, X_train, y_train, n_repeats=n_repeats, random_state=random_state, n_jobs=n_jobs)
 
     # Create a DataFrame for permutation importances
@@ -1387,40 +1509,13 @@ def ml_analysis(df, channel_of_interest=3, location_column='column_name', positi
     else:
         feature_importance_df = pd.DataFrame()
 
-    # Predicting the target variable for the test set
-    predictions_test = model.predict(X_test)
-    combined_df.loc[X_test.index, 'predictions'] = predictions_test
-
-    # Get prediction probabilities for the test set
-    prediction_probabilities_test = model.predict_proba(X_test)
-
-    # Find the optimal threshold
-    optimal_threshold = find_optimal_threshold(y_test, prediction_probabilities_test[:, 1])
-    if verbose:
-        print(f'Optimal threshold: {optimal_threshold}')
-
-    # Predicting the target variable for all other rows in the dataframe
-    X_all = df[features]
-    all_predictions = model.predict(X_all)
-    df['predictions'] = all_predictions
-
-    # Get prediction probabilities for all rows in the dataframe
-    prediction_probabilities = model.predict_proba(X_all)
-    for i in range(prediction_probabilities.shape[1]):
-        df[f'prediction_probability_class_{i}'] = prediction_probabilities[:, i]
-    if verbose:
-        print("\nClassification Report:")
-        print(classification_report(y_test, predictions_test))
-    report_dict = classification_report(y_test, predictions_test, output_dict=True)
-    metrics_df = pd.DataFrame(report_dict).transpose()
-
     df = _calculate_similarity(df, features, location_column, positive_control, negative_control)
 
     df['prcfo'] = df.index.astype(str)
     df[['plate', 'row_name', 'column_name', 'field', 'object']] = df['prcfo'].str.split('_', expand=True)
     df['prc'] = df['plate'] + '_' + df['row_name'] + '_' + df['column_name']
     
-    return [df, permutation_df, feature_importance_df, model, X_train, X_test, y_train, y_test, metrics_df], [permutation_fig, feature_importance_fig]
+    return [df, permutation_df, feature_importance_df, model, X_train, X_test, y_train, y_test, metrics_df, features], [permutation_fig, feature_importance_fig]
 
 def shap_analysis(model, X_train, X_test):
     
@@ -1495,9 +1590,9 @@ def _calculate_similarity(df, features, col_to_compare, val1, val2):
         inv_cov_matrix = np.linalg.inv(cov_matrix + np.eye(cov_matrix.shape[0]) * epsilon)
         
     # Calculate similarity scores
-    def safe_similarity(func, row, control):
+    def safe_similarity(func, row, control, *args, **kwargs):
         try:
-            return func(row, control)
+            return func(row, control, *args, **kwargs)
         except Exception:
             return np.nan
         

spacr/settings.py

@@ -283,7 +283,10 @@ def set_default_analyze_screen(settings):
     settings.setdefault('cmap','viridis')
     settings.setdefault('channel_of_interest',3)
     settings.setdefault('minimum_cell_count',25)
-    settings.setdefault('n_estimators',100)
+    settings.setdefault('reg_alpha',0.1)
+    settings.setdefault('reg_lambda',1.0)
+    settings.setdefault('learning_rate',0.001)
+    settings.setdefault('n_estimators',1000)
     settings.setdefault('test_size',0.2)
     settings.setdefault('location_column','column_name')
     settings.setdefault('positive_control','c2')
@@ -296,6 +299,8 @@ def set_default_analyze_screen(settings):
     settings.setdefault('remove_low_variance_features',True)
     settings.setdefault('remove_highly_correlated_features',True)
     settings.setdefault('n_jobs',-1)
+    settings.setdefault('prune_features',False)
+    settings.setdefault('cross_validation',True)
     settings.setdefault('verbose',True)
     return settings
 
@@ -872,10 +877,40 @@ expected_types = {
     "target_layer":str,
     "save_to_db":bool,
     "test_mode":bool,
+    "test_images":int,
+    "remove_background_cell":bool,
+    "remove_background_nucleus":bool,
+    "remove_background_pathogen":bool,
+    "figuresize":int,
+    "cmap":str,
+    "pathogen_model":str,
     "normalize_input":bool,
+    "filter_column":str,
+    "target_unique_count":int,
+    "threshold_multiplier":int,
+    "threshold_method":str,
+    "count_data":list,
+    "score_data":list,
+    "min_n":int,
+    "controls":list,
+    "toxo":bool,
+    "volcano":str,
+    "metadata_files":list,
+    "filter_value":list,
+    "split_axis_lims":str,
+    "x_lim":(list,None),
+    "log_x":bool,
+    "log_y":bool,
+    "reg_alpha":(int,float),
+    "reg_lambda":(int,float),
+    "prune_features":bool,
+    "cross_validation":bool,
+    "offset_start":int,
+    "chunk_size":int,
+    "single_direction":str,
 }
 
-categories = {"Paths":[ "src", "grna", "barcodes", "custom_model_path", "dataset","model_path","grna_csv","row_csv","column_csv"],
+categories = {"Paths":[ "src", "grna", "barcodes", "custom_model_path", "dataset","model_path","grna_csv","row_csv","column_csv", "metadata_files", "score_data","count_data"],
              "General": ["metadata_type", "custom_regex", "experiment", "channels", "magnification", "channel_dims", "apply_model_to_dataset", "generate_training_dataset", "train_DL_model", "segmentation_mode"],
              "Cellpose":["fill_in","from_scratch", "n_epochs", "width_height", "model_name", "custom_model", "resample", "rescale", "CP_prob", "flow_threshold", "percentiles", "invert", "diameter", "grayscale", "Signal_to_noise", "resize", "target_height", "target_width"],
              "Cell": ["cell_intensity_range", "cell_size_range", "cell_chann_dim", "cell_channel", "cell_background", "cell_Signal_to_noise", "cell_CP_prob", "cell_FT", "remove_background_cell", "cell_min_size", "cell_mask_dim", "cytoplasm", "cytoplasm_min_size", "uninfected", "merge_edge_pathogen_cells", "adjust_cells", "cells", "cell_loc"],
@@ -883,18 +918,18 @@ categories = {"Paths":[ "src", "grna", "barcodes", "custom_model_path", "dataset
              "Pathogen": ["pathogen_intensity_range", "pathogen_size_range", "pathogen_chann_dim", "pathogen_channel", "pathogen_background", "pathogen_Signal_to_noise", "pathogen_CP_prob", "pathogen_FT", "pathogen_model", "remove_background_pathogen", "pathogen_min_size", "pathogen_mask_dim", "pathogens", "pathogen_loc", "pathogen_types", "pathogen_plate_metadata", ],
              "Measurements": ["remove_image_canvas", "remove_highly_correlated", "homogeneity", "homogeneity_distances", "radial_dist", "calculate_correlation", "manders_thresholds", "save_measurements", "tables", "image_nr", "dot_size", "filter_by", "remove_highly_correlated_features", "remove_low_variance_features", "channel_of_interest"],
              "Object Image": ["save_png", "dialate_pngs", "dialate_png_ratios", "png_size", "png_dims", "save_arrays", "normalize_by", "crop_mode", "normalize", "use_bounding_box"],
-             "Sequencing": ["signal_direction","mode","comp_level","comp_type","save_h5","expected_end","offset","target_sequence","regex", "highlight"],
+             "Sequencing": ["offset_start","chunk_size","single_direction", "signal_direction","mode","comp_level","comp_type","save_h5","expected_end","offset","target_sequence","regex", "highlight"],
              "Generate Dataset":["save_to_db","file_metadata","class_metadata", "annotation_column","annotated_classes", "dataset_mode", "metadata_type_by","custom_measurement", "sample", "size"],
              "Hyperparamiters (Training)": ["png_type", "score_threshold","file_type", "train_channels", "epochs", "loss_type", "optimizer_type","image_size","val_split","learning_rate","weight_decay","dropout_rate", "init_weights", "train", "classes", "augment", "amsgrad","use_checkpoint","gradient_accumulation","gradient_accumulation_steps","intermedeate_save","pin_memory"],
              "Hyperparamiters (Embedding)": ["visualize","n_neighbors","min_dist","metric","resnet_features","reduction_method","embedding_by_controls","col_to_compare","log_data"],
              "Hyperparamiters (Clustering)": ["eps","min_samples","analyze_clusters","clustering","remove_cluster_noise"],
-             "Hyperparamiters (Regression)":["cov_type", "class_1_threshold", "plate", "other", "fraction_threshold", "alpha", "random_row_column_effects", "regression_type", "min_cell_count", "agg_type", "transform", "dependent_variable"],
+             "Hyperparamiters (Regression)":["cross_validation","prune_features","reg_lambda","reg_alpha","cov_type", "class_1_threshold", "plate", "other", "fraction_threshold", "alpha", "random_row_column_effects", "regression_type", "min_cell_count", "agg_type", "transform", "dependent_variable"],
              "Hyperparamiters (Activation)":["cam_type", "overlay", "correlation", "target_layer", "normalize_input"],
-             "Annotation": ["nc_loc", "pc_loc", "nc", "pc", "cell_plate_metadata","treatment_plate_metadata", "metadata_types", "cell_types", "target","positive_control","negative_control", "location_column", "treatment_loc", "channel_of_interest", "measurement", "treatments", "um_per_pixel", "nr_imgs", "exclude", "exclude_conditions", "mix", "pos", "neg"],
-             "Plot": ["plot", "plot_control", "plot_nr", "examples_to_plot", "normalize_plots", "cmap", "figuresize", "plot_cluster_grids", "img_zoom", "row_limit", "color_by", "plot_images", "smooth_lines", "plot_points", "plot_outlines", "black_background", "plot_by_cluster", "heatmap_feature","grouping","min_max","cmap","save_figure"],
+             "Annotation": ["filter_column", "filter_value","volcano", "toxo", "controls", "nc_loc", "pc_loc", "nc", "pc", "cell_plate_metadata","treatment_plate_metadata", "metadata_types", "cell_types", "target","positive_control","negative_control", "location_column", "treatment_loc", "channel_of_interest", "measurement", "treatments", "um_per_pixel", "nr_imgs", "exclude", "exclude_conditions", "mix", "pos", "neg"],
+             "Plot": ["plot", "split_axis_lims", "x_lim","log_x","log_y", "plot_control", "plot_nr", "examples_to_plot", "normalize_plots", "cmap", "figuresize", "plot_cluster_grids", "img_zoom", "row_limit", "color_by", "plot_images", "smooth_lines", "plot_points", "plot_outlines", "black_background", "plot_by_cluster", "heatmap_feature","grouping","min_max","cmap","save_figure"],
              "Test": ["test_mode", "test_images", "random_test", "test_nr", "test", "test_split"],
              "Timelapse": ["timelapse", "fps", "timelapse_displacement", "timelapse_memory", "timelapse_frame_limits", "timelapse_remove_transient", "timelapse_mode", "timelapse_objects", "compartments"],
-             "Advanced": ["shuffle", "target_intensity_min", "cells_per_well", "nuclei_limit", "pathogen_limit", "background", "backgrounds", "schedule", "test_size","exclude","n_repeats","top_features", "model_type_ml", "model_type","minimum_cell_count","n_estimators","preprocess", "remove_background", "normalize", "lower_percentile", "merge_pathogens", "batch_size", "filter", "save", "masks", "verbose", "randomize", "n_jobs"],
+             "Advanced": ["target_unique_count","threshold_multiplier", "threshold_method", "min_n","shuffle", "target_intensity_min", "cells_per_well", "nuclei_limit", "pathogen_limit", "background", "backgrounds", "schedule", "test_size","exclude","n_repeats","top_features", "model_type_ml", "model_type","minimum_cell_count","n_estimators","preprocess", "remove_background", "normalize", "lower_percentile", "merge_pathogens", "batch_size", "filter", "save", "masks", "verbose", "randomize", "n_jobs"],
              "Miscellaneous": ["all_to_mip", "pick_slice", "skip_mode", "upscale", "upscale_factor"]
              }
 

spacr/utils.py

@@ -4277,6 +4277,12 @@ def filter_dataframe_features(df, channel_of_interest, exclude=None, remove_low_
     
     if remove_highly_correlated_features:
         df = remove_highly_correlated_columns(df, threshold=0.95, verbose=verbose)
+        
+    # Remove columns with NaN values
+    before_drop_NaN = len(df.columns)
+    df = df.dropna(axis=1)
+    after_drop_NaN = len(df.columns)
+    print(f"Dropped {before_drop_NaN - after_drop_NaN} columns with NaN values")
 
     # Select numerical features
     features = df.select_dtypes(include=[np.number]).columns.tolist()
@@ -4759,7 +4765,8 @@ def get_ml_results_paths(src, model_type='xgboost', channel_of_interest=1):
     shap_fig_path = os.path.join(res_fldr, 'shap.pdf')
     plate_heatmap_path = os.path.join(res_fldr, 'plate_heatmap.pdf')
     settings_csv = os.path.join(res_fldr, 'ml_settings.csv')
-    return data_path, permutation_path, feature_importance_path, model_metricks_path, permutation_fig_path, feature_importance_fig_path, shap_fig_path, plate_heatmap_path, settings_csv
+    ml_features = os.path.join(res_fldr, 'ml_features.csv')
+    return data_path, permutation_path, feature_importance_path, model_metricks_path, permutation_fig_path, feature_importance_fig_path, shap_fig_path, plate_heatmap_path, settings_csv, ml_features
 
 def augment_image(image):
     """
@@ -5038,19 +5045,22 @@ def generate_cytoplasm_mask(nucleus_mask, cell_mask):
     return cytoplasm_mask
 
 def add_column_to_database(settings):
-    """
-    Adds a new column to the database table by matching on a common column from the DataFrame.
-    If the column already exists in the database, it adds the column with a suffix.
-    NaN values will remain as NULL in the database.
-    
-    Parameters:
-    - settings: A dictionary containing the following keys:
-        - 'csv_path': Path to the CSV file with the data to be added.
-        - 'db_path': Path to the SQLite database (or connection string for other databases).
-        - 'table_name': The name of the table in the database.
-        - 'update_column': The name of the new column in the DataFrame to add to the database.
-        - 'match_column': The common column used to match rows.
-    """
+    #"""
+    #Adds a new column to the database table by matching on a common column from the DataFrame.
+    #If the column already exists in the database, it adds the column with a suffix.
+    #NaN values will remain as NULL in the database.
+
+    #Parameters:
+    #    settings (dict): A dictionary containing the following keys:
+    #    csv_path (str): Path to the CSV file with the data to be added.
+    #    db_path (str): Path to the SQLite database (or connection string for other databases).
+    #    table_name (str): The name of the table in the database.
+    #    update_column (str): The name of the new column in the DataFrame to add to the database.
+    #    match_column (str): The common column used to match rows.
+
+    #Returns:
+    #    None
+    #"""
 
     # Read the DataFrame from the provided CSV path
     df = pd.read_csv(settings['csv_path'])

{spacr-0.3.72.dist-info → spacr-0.3.81.dist-info}/METADATA

@@ -1,6 +1,6 @@
 Metadata-Version: 2.1
 Name: spacr
-Version: 0.3.72
+Version: 0.3.81
 Summary: Spatial phenotype analysis of crisp screens (SpaCr)
 Home-page: https://github.com/EinarOlafsson/spacr
 Author: Einar Birnir Olafsson

{spacr-0.3.72.dist-info → spacr-0.3.81.dist-info}/RECORD

@@ -12,24 +12,24 @@ spacr/chat_bot.py,sha256=n3Fhqg3qofVXHmh3H9sUcmfYy9MmgRnr48663MVdY9E,1244
 spacr/core.py,sha256=3u2qKmPmTlswvE1uKTF4gi7KQ3sJBHV9No_ysgk7JCU,48487
 spacr/deep_spacr.py,sha256=V3diLyxX-0_F5UxhX_b94ROOvL9eoLvnoUmF3nMBqPQ,43250
 spacr/gui.py,sha256=ARyn9Q_g8HoP-cXh1nzMLVFCKqthY4v2u9yORyaQqQE,8230
-spacr/gui_core.py,sha256=N7R7yvfK_dJhOReM_kW3Ci8Bokhi1OzsxeKqvSGdvV4,41460
-spacr/gui_elements.py,sha256=EKlvEg_4_je7jciEdR3NTgPrcTraowa2e2RUt-xqd6M,138254
+spacr/gui_core.py,sha256=3S1S7rOtGfm5Z-Fb-jk1HxjsLZo6IboBxtXtsDMw1ME,46578
+spacr/gui_elements.py,sha256=fc9z-GP_PmtJy2lIsfi3fx5r5puTJ-zz8ntB9pC53Gc,138246
 spacr/gui_utils.py,sha256=u9RoIOWpAXFEOnUlLpMQZrc1pWSg6omZsJMIhJdRv_g,41211
 spacr/io.py,sha256=LF6lpphw7GSeuoHQijPykjKNF56wNTFEWFZuDQp3O6Q,145739
 spacr/logger.py,sha256=lJhTqt-_wfAunCPl93xE65Wr9Y1oIHJWaZMjunHUeIw,1538
 spacr/measure.py,sha256=2lK-ZcTxLM-MpXV1oZnucRD9iz5aprwahRKw9IEqshg,55085
 spacr/mediar.py,sha256=FwLvbLQW5LQzPgvJZG8Lw7GniA2vbZx6Jv6vIKu7I5c,14743
-spacr/ml.py,sha256=h0IrXoNnyNzZLPYbtZPFI6c4Qeu1gH8R3iUz_O7-ar0,78114
+spacr/ml.py,sha256=x19S8OsR5omb8e6MU9I99Nz95J_QvM5siyk-zaAU3p8,82866
 spacr/openai.py,sha256=5vBZ3Jl2llYcW3oaTEXgdyCB2aJujMUIO5K038z7w_A,1246
 spacr/plot.py,sha256=gXC7y3uT4sx8KRODeSFWQG_A1CylsuJ5B7HYe_un6so,165177
 spacr/sequencing.py,sha256=ClUfwPPK6rNUbUuiEkzcwakzVyDKKUMv9ricrxT8qQY,25227
-spacr/settings.py,sha256=14PFxw3YK9tUqbaC6BqfbrWk3sN7gyTZAAI8KNy5KBA,80461
+spacr/settings.py,sha256=3bgBWBotIO7TZx2nh6JoEaqHNmwbChAiz1gW4xmURQs,81788
 spacr/sim.py,sha256=1xKhXimNU3ukzIw-3l9cF3Znc_brW8h20yv8fSTzvss,71173
 spacr/stats.py,sha256=mbhwsyIqt5upsSD346qGjdCw7CFBa0tIS7zHU9e0jNI,9536
 spacr/submodules.py,sha256=SK8YEs850LAx30YAiwap7ecLpp1_p-bci6H-Or0GLoA,55500
 spacr/timelapse.py,sha256=KGfG4L4-QnFfgbF7L6C5wL_3gd_rqr05Foje6RsoTBg,39603
 spacr/toxo.py,sha256=z2nT5aAze3NUIlwnBQcnkARihDwoPfqOgQIVoUluyK0,25087
-spacr/utils.py,sha256=LX2Hu6QC-yG9ZVBiM2dkSN9yytCB0eTTRGfExiZzYzE,221940
+spacr/utils.py,sha256=jjZIqzJKl9nnWgj2eJiLFT27gED8hO6rAEsJLlimm-E,222298
 spacr/version.py,sha256=axH5tnGwtgSnJHb5IDhiu4Zjk5GhLyAEDRe-rnaoFOA,409
 spacr/resources/MEDIAR/.gitignore,sha256=Ff1q9Nme14JUd-4Q3jZ65aeQ5X4uttptssVDgBVHYo8,152
 spacr/resources/MEDIAR/LICENSE,sha256=yEj_TRDLUfDpHDNM0StALXIt6mLqSgaV2hcCwa6_TcY,1065
@@ -152,9 +152,9 @@ spacr/resources/icons/umap.png,sha256=dOLF3DeLYy9k0nkUybiZMe1wzHQwLJFRmgccppw-8b
 spacr/resources/images/plate1_E01_T0001F001L01A01Z01C02.tif,sha256=Tl0ZUfZ_AYAbu0up_nO0tPRtF1BxXhWQ3T3pURBCCRo,7958528
 spacr/resources/images/plate1_E01_T0001F001L01A02Z01C01.tif,sha256=m8N-V71rA1TT4dFlENNg8s0Q0YEXXs8slIn7yObmZJQ,7958528
 spacr/resources/images/plate1_E01_T0001F001L01A03Z01C03.tif,sha256=Pbhk7xn-KUP6RSIhJsxQcrHFImBm3GEpLkzx7WOc-5M,7958528
-spacr-0.3.72.dist-info/LICENSE,sha256=SR-2MeGc6SCM1UORJYyarSWY_A-JaOMFDj7ReSs9tRM,1083
-spacr-0.3.72.dist-info/METADATA,sha256=Kt166mcmw6Hb0u47_tZVq1EiZuK3Z_aDC0T7jE41dnI,6032
-spacr-0.3.72.dist-info/WHEEL,sha256=HiCZjzuy6Dw0hdX5R3LCFPDmFS4BWl8H-8W39XfmgX4,91
-spacr-0.3.72.dist-info/entry_points.txt,sha256=BMC0ql9aNNpv8lUZ8sgDLQMsqaVnX5L535gEhKUP5ho,296
-spacr-0.3.72.dist-info/top_level.txt,sha256=GJPU8FgwRXGzKeut6JopsSRY2R8T3i9lDgya42tLInY,6
-spacr-0.3.72.dist-info/RECORD,,
+spacr-0.3.81.dist-info/LICENSE,sha256=SR-2MeGc6SCM1UORJYyarSWY_A-JaOMFDj7ReSs9tRM,1083
+spacr-0.3.81.dist-info/METADATA,sha256=TneYaiWWfNHUuqpSQpRAEzWzYia6KIksYDzNVDcsimw,6032
+spacr-0.3.81.dist-info/WHEEL,sha256=HiCZjzuy6Dw0hdX5R3LCFPDmFS4BWl8H-8W39XfmgX4,91
+spacr-0.3.81.dist-info/entry_points.txt,sha256=BMC0ql9aNNpv8lUZ8sgDLQMsqaVnX5L535gEhKUP5ho,296
+spacr-0.3.81.dist-info/top_level.txt,sha256=GJPU8FgwRXGzKeut6JopsSRY2R8T3i9lDgya42tLInY,6
+spacr-0.3.81.dist-info/RECORD,,