spacr 0.1.1__py3-none-any.whl → 0.1.12__py3-none-any.whl

spacr/measure_app.py

@@ -0,0 +1,246 @@
+import sys, traceback, matplotlib, ctypes
+import tkinter as tk
+from tkinter import ttk, scrolledtext
+from matplotlib.figure import Figure
+from matplotlib.backends.backend_tkagg import FigureCanvasTkAgg
+matplotlib.use('Agg')  # Use the non-GUI Agg backend
+from multiprocessing import Process, Queue, Value
+from tkinter import filedialog
+
+try:
+    ctypes.windll.shcore.SetProcessDpiAwareness(True)
+except AttributeError:
+    pass
+
+from .logger import log_function_call
+from .gui_utils import ScrollableFrame, StdoutRedirector, CustomButton, ToggleSwitch, ToolTip
+from .gui_utils import process_stdout_stderr, set_dark_style, set_default_font, generate_fields, main_thread_update_function, create_menu_bar
+from .gui_utils import measure_variables, measure_crop_wrapper, clear_canvas, check_measure_gui_settings, read_settings_from_csv, update_settings_from_csv, style_text_boxes
+
+thread_control = {"run_thread": None, "stop_requested": False}
+
+def import_settings(scrollable_frame):
+    global vars_dict
+
+    csv_file_path = filedialog.askopenfilename(filetypes=[("CSV files", "*.csv")])
+    csv_settings = read_settings_from_csv(csv_file_path)
+    variables = measure_variables()
+    new_settings = update_settings_from_csv(variables, csv_settings)
+    vars_dict = generate_fields(new_settings, scrollable_frame)
+
+def toggle_test_mode():
+    global vars_dict
+    current_state = vars_dict['test_mode'][2].get()
+    new_state = not current_state
+    vars_dict['test_mode'][2].set(new_state)
+    if new_state:
+        test_mode_button.config(bg="blue")
+    else:
+        test_mode_button.config(bg="gray")
+
+def toggle_advanced_settings():
+    global vars_dict
+
+    timelapse_settings = ['timelapse', 'timelapse_objects']
+    misc_settings = ['representative_images', 'plot', 'plot_filtration', 'include_uninfected', 'dialate_pngs', 'dialate_png_ratios']
+    opperational_settings = ['max_workers','experiment','cells','cell_loc','pathogens','pathogen_loc','treatments','treatment_loc','channel_of_interest','compartments','measurement','nr_imgs', 'um_per_pixel']
+    
+    advanced_settings = timelapse_settings+misc_settings+opperational_settings
+
+    # Toggle visibility of advanced settings
+    for setting in advanced_settings:
+        label, widget, var = vars_dict[setting]
+        if advanced_var.get() is False:
+            label.grid_remove()  # Hide the label
+            widget.grid_remove()  # Hide the widget
+        else:
+            label.grid()  # Show the label
+            widget.grid()  # Show the widget
+
+#@log_function_call
+def run_measure_gui(q, fig_queue, stop_requested):
+    global vars_dict
+    process_stdout_stderr(q)
+    try:
+        print('hello')
+        settings = check_measure_gui_settings(vars_dict)
+        measure_crop_wrapper(settings=settings, q=q, fig_queue=fig_queue)
+    except Exception as e:
+        q.put(f"Error during processing: {e}")
+        traceback.print_exc()
+    finally:
+        stop_requested.value = 1
+
+#@log_function_call
+def start_process(q, fig_queue):
+    global thread_control
+    if thread_control.get("run_thread") is not None:
+        initiate_abort()
+
+    stop_requested = Value('i', 0)  # multiprocessing shared value for inter-process communication
+    thread_control["stop_requested"] = stop_requested
+    thread_control["run_thread"] = Process(target=run_measure_gui, args=(q, fig_queue, stop_requested))
+    thread_control["run_thread"].start()
+
+#@log_function_call
+def initiate_abort():
+    global thread_control
+    if thread_control.get("stop_requested") is not None:
+        thread_control["stop_requested"].value = 1
+
+    if thread_control.get("run_thread") is not None:
+        thread_control["run_thread"].join(timeout=5)
+        if thread_control["run_thread"].is_alive():
+            thread_control["run_thread"].terminate()
+        thread_control["run_thread"] = None
+
+#@log_function_call
+def initiate_measure_root(parent_frame):
+    global vars_dict, q, canvas, fig_queue, canvas_widget, thread_control, variables, advanced_var, scrollable_frame
+    
+    style = ttk.Style(parent_frame)
+    set_dark_style(style)
+    style_text_boxes(style)
+    set_default_font(parent_frame, font_name="Helvetica", size=8)
+
+    parent_frame.configure(bg='black')
+    parent_frame.grid_rowconfigure(0, weight=1)
+    parent_frame.grid_columnconfigure(0, weight=1)
+
+    fig_queue = Queue()
+    
+    # Initialize after_tasks if not already done
+    if not hasattr(parent_frame, 'after_tasks'):
+        parent_frame.after_tasks = []
+
+    def _process_fig_queue():
+        global canvas
+        try:
+            while not fig_queue.empty():
+                clear_canvas(canvas)
+                fig = fig_queue.get_nowait()
+                for ax in fig.get_axes():
+                    ax.set_xticks([])  # Remove x-axis ticks
+                    ax.set_yticks([])  # Remove y-axis ticks
+                    ax.xaxis.set_visible(False)  # Hide the x-axis
+                    ax.yaxis.set_visible(False)  # Hide the y-axis
+                fig.tight_layout()
+                fig.set_facecolor('black')
+                canvas.figure = fig
+                fig_width, fig_height = canvas_widget.winfo_width(), canvas_widget.winfo_height()
+                fig.set_size_inches(fig_width / fig.dpi, fig_height / fig.dpi, forward=True)
+                canvas.draw_idle()
+        except Exception as e:
+            traceback.print_exc()
+        finally:
+            after_id = canvas_widget.after(100, _process_fig_queue)
+            parent_frame.after_tasks.append(after_id)
+
+    def _process_console_queue():
+        while not q.empty():
+            message = q.get_nowait()
+            console_output.insert(tk.END, message)
+            console_output.see(tk.END)
+        after_id = console_output.after(100, _process_console_queue)
+        parent_frame.after_tasks.append(after_id)
+
+    # Clear previous content if any
+    for widget in parent_frame.winfo_children():
+        widget.destroy()
+
+    vertical_container = tk.PanedWindow(parent_frame, orient=tk.HORIZONTAL)
+    vertical_container.grid(row=0, column=0, sticky=tk.NSEW)
+    parent_frame.grid_rowconfigure(0, weight=1)
+    parent_frame.grid_columnconfigure(0, weight=1)
+
+    # Settings Section
+    settings_frame = tk.Frame(vertical_container, bg='black')
+    vertical_container.add(settings_frame, stretch="always")
+    settings_label = ttk.Label(settings_frame, text="Settings", background="black", foreground="white")
+    settings_label.grid(row=0, column=0, pady=10, padx=10)
+    scrollable_frame = ScrollableFrame(settings_frame, width=500)
+    scrollable_frame.grid(row=1, column=0, sticky="nsew")
+    settings_frame.grid_rowconfigure(1, weight=1)
+    settings_frame.grid_columnconfigure(0, weight=1)
+    
+    # Create advanced settings checkbox
+    advanced_var = tk.BooleanVar(value=False)
+    advanced_Toggle = ToggleSwitch(scrollable_frame.scrollable_frame, text="Advanced Settings", variable=advanced_var, command=toggle_advanced_settings)
+    advanced_Toggle.grid(row=48, column=0, pady=10, padx=10)
+    variables = measure_variables()
+    vars_dict = generate_fields(variables, scrollable_frame)
+    toggle_advanced_settings()
+    vars_dict['Test mode'] = (None, None, tk.BooleanVar(value=False))
+    
+    # Button section
+    test_mode_button = CustomButton(scrollable_frame.scrollable_frame, text="Test Mode", command=toggle_test_mode)
+    test_mode_button.grid(row=47, column=1, pady=10, padx=10)
+    import_btn = CustomButton(scrollable_frame.scrollable_frame, text="Import", command=lambda: import_settings(scrollable_frame), font=('Helvetica', 10))
+    import_btn.grid(row=47, column=0, pady=20, padx=20)
+    run_button = CustomButton(scrollable_frame.scrollable_frame, text="Run", command=lambda: start_process(q, fig_queue), font=('Helvetica', 10))
+    run_button.grid(row=45, column=0, pady=20, padx=20)
+    abort_button = CustomButton(scrollable_frame.scrollable_frame, text="Abort", command=initiate_abort, font=('Helvetica', 10))
+    abort_button.grid(row=45, column=1, pady=20, padx=20)
+    progress_label = ttk.Label(scrollable_frame.scrollable_frame, text="Processing: 0%", background="black", foreground="white") # Create progress field
+    progress_label.grid(row=50, column=0, columnspan=2, sticky="ew", pady=(5, 0), padx=10)
+
+    # Plot Canvas Section
+    plot_frame = tk.PanedWindow(vertical_container, orient=tk.VERTICAL)
+    vertical_container.add(plot_frame, stretch="always")
+    figure = Figure(figsize=(30, 4), dpi=100, facecolor='black')
+    plot = figure.add_subplot(111)
+    plot.plot([], [])
+    plot.axis('off')
+    canvas = FigureCanvasTkAgg(figure, master=plot_frame)
+    canvas.get_tk_widget().configure(cursor='arrow', background='black', highlightthickness=0)
+    canvas_widget = canvas.get_tk_widget()
+    plot_frame.add(canvas_widget, stretch="always")
+    canvas.draw()
+    canvas.figure = figure
+
+    # Console Section
+    console_frame = tk.Frame(vertical_container, bg='black')
+    vertical_container.add(console_frame, stretch="always")
+    console_label = ttk.Label(console_frame, text="Console", background="black", foreground="white")
+    console_label.grid(row=0, column=0, pady=10, padx=10)
+    console_output = scrolledtext.ScrolledText(console_frame, height=10, bg='black', fg='white', insertbackground='white')
+    console_output.grid(row=1, column=0, sticky="nsew")
+    console_frame.grid_rowconfigure(1, weight=1)
+    console_frame.grid_columnconfigure(0, weight=1)
+
+    q = Queue()
+    sys.stdout = StdoutRedirector(console_output)
+    sys.stderr = StdoutRedirector(console_output)
+    
+    _process_console_queue()
+    _process_fig_queue()
+    
+    after_id = parent_frame.after(100, lambda: main_thread_update_function(parent_frame, q, fig_queue, canvas_widget, progress_label))
+    parent_frame.after_tasks.append(after_id)
+    
+    return parent_frame, vars_dict
+
+def gui_measure():
+    root = tk.Tk()
+    width = root.winfo_screenwidth()
+    height = root.winfo_screenheight()
+    root.geometry(f"{width}x{height}") 
+    root.title("SpaCr: measure objects")
+    
+    # Clear previous content if any
+    if hasattr(root, 'content_frame'):
+        for widget in root.content_frame.winfo_children():
+            widget.destroy()
+        root.content_frame.grid_forget()
+    else:
+        root.content_frame = tk.Frame(root)
+        root.content_frame.grid(row=1, column=0, sticky="nsew")
+        root.grid_rowconfigure(1, weight=1)
+        root.grid_columnconfigure(0, weight=1)
+    
+    initiate_measure_root(root.content_frame)
+    create_menu_bar(root)
+    root.mainloop()
+
+if __name__ == "__main__":
+    gui_measure()

{spacr-0.1.1.dist-info → spacr-0.1.12.dist-info}/METADATA

@@ -1,6 +1,6 @@
 Metadata-Version: 2.1
 Name: spacr
-Version: 0.1.1
+Version: 0.1.12
 Summary: Spatial phenotype analysis of crisp screens (SpaCr)
 Home-page: https://github.com/EinarOlafsson/spacr
 Author: Einar Birnir Olafsson
@@ -40,6 +40,10 @@ Requires-Dist: ttf-opensans >=2020.10.30
 Requires-Dist: customtkinter <6.0,>=5.2.2
 Requires-Dist: biopython <2.0,>=1.80
 Requires-Dist: lxml <6.0,>=5.1.0
+Requires-Dist: qtpy <2.5,>=2.4.1
+Requires-Dist: superqt <0.7,>=0.6.7
+Requires-Dist: pyqt6 <6.8,>=6.7.1
+Requires-Dist: pyqtgraph <0.14,>=0.13.7
 Provides-Extra: dev
 Requires-Dist: pytest >=3.9 ; extra == 'dev'
 Provides-Extra: full

spacr-0.1.12.dist-info/RECORD

@@ -0,0 +1,54 @@
+spacr/__init__.py,sha256=xu2zNv8A523YvDACynkThH2CYqN5mafsu9lfKGjMmIM,1278
+spacr/__main__.py,sha256=bkAJJD2kjIqOP-u1kLvct9jQQCeUXzlEjdgitwi1Lm8,75
+spacr/alpha.py,sha256=Y95sLEfpK2OSYKRn3M8eUOU33JJeXfV8zhrC4KnwSTY,35244
+spacr/annotate_app.py,sha256=imQ7ZEXDyM6ce1dxZ1xUS1-KequuF_NCI4xCaPLjvco,29275
+spacr/annotate_app_v2.py,sha256=imQ7ZEXDyM6ce1dxZ1xUS1-KequuF_NCI4xCaPLjvco,29275
+spacr/app_annotate.py,sha256=I0c-Q5WYkA2lCKyNByACM1YzSdLC17X2mNJ2XAnWgNQ,23619
+spacr/app_classify.py,sha256=052t5MeUsP6IdJTA_uWY4AmyBHTkf8_KdslfDWtP7pk,8428
+spacr/app_make_masks.py,sha256=fIlWG13PMrafvwj92el1Puo48CJy4Q3iINOXa5mW3oI,45136
+spacr/app_make_masks_v2.py,sha256=-HhEgi0ber1Jyjp5RgqUcZM4vPvGXPKh1pv3SLyVZoU,30574
+spacr/app_mask.py,sha256=kp0Zvw9uA-Z8FfuKChrXo1DA01_H7fdKCsFZzmKKrtI,10731
+spacr/app_measure.py,sha256=85ux3Udw0GRjr7h2XoID752wvQCTtdHsfFSQOwWOkV0,10623
+spacr/chris.py,sha256=YlBjSgeZaY8HPy6jkrT_ISAnCMAKVfvCxF0I9eAZLFM,2418
+spacr/classify_app.py,sha256=Zi15ryc1ocYitRF4kyxlC27XxGyzfSPdvj2d6ZrSh7E,8446
+spacr/cli.py,sha256=507jfOOEV8BoL4eeUcblvH-iiDHdBrEVJLu1ghAAPSc,1800
+spacr/core.py,sha256=m9fsk-qDPow4AzOYpTIsd4jT7PF_L_4y5xillR5eRdk,160253
+spacr/deep_spacr.py,sha256=N0o7ILD2p1FTfU4DFxnpjs00xjLhwib-ev0XGqA6muU,37035
+spacr/foldseek.py,sha256=YIP1d4Ci6CeA9jSyiv-HTDbNmAmcSM9Y_DaOs7wYzLY,33546
+spacr/get_alfafold_structures.py,sha256=ehx_MQgb12k3hFecP6cYVlm5TLO8iWjgevy8ESyS3cw,3544
+spacr/graph_learning.py,sha256=1tR-ZxvXE3dBz1Saw7BeVFcrsUFu9OlUZeZVifih9eo,13070
+spacr/gui.py,sha256=nwRqK3DLYrJQh4iCoyfJMpwSuxCn1xc4zHLmQ8cW8Is,6519
+spacr/gui_2.py,sha256=ZAI5quQYbhQJ40vK0NCqU_UMSPLkpfeQpomBWUSM0fc,6946
+spacr/gui_annotate.py,sha256=ugBksLGOHdtOLlEuRyyc59TrkYKu3rDf8JxEgiBSVao,6536
+spacr/gui_classify_app.py,sha256=Zi15ryc1ocYitRF4kyxlC27XxGyzfSPdvj2d6ZrSh7E,8446
+spacr/gui_make_masks_app.py,sha256=tl4M4Q2WQgrrwjRBJVevxJxpNowqzPhWkdCOm2UfRbw,45053
+spacr/gui_make_masks_app_v2.py,sha256=X3izTBXdCZDlkVe-fbG-jmCQtcAbmK0OIivjyWaLhug,30576
+spacr/gui_mask_app.py,sha256=mhTl_XzXLFl8Tx3WYEMpdYB_qw9u5JJa0EdkvlcIzAE,10706
+spacr/gui_measure_app.py,sha256=_C1-XFL5HSquUEEbM_NcxdvHx-socPFCx85MBG4d6xo,10598
+spacr/gui_sim_app.py,sha256=47DEQpj8HBSa-_TImW-5JCeuQeRkm5NMpJWZG3hSuFU,0
+spacr/gui_utils.py,sha256=n_juaV_-DspNab9NNncWauB9wKCd7lPcvoKsjKfWFrk,56436
+spacr/io.py,sha256=IoERqSwoxJrInYl-E0WfwFOEDZXFdJofk5DmpbyLGWM,112077
+spacr/logger.py,sha256=7Zqr3TuuOQLWT32gYr2q1qvv7x0a2JhLANmZcnBXAW8,670
+spacr/make_masks_app.py,sha256=iGaTwhowoe2JMOSOf8bJwQZTooRhLQx7KO0ewnAmqDY,45138
+spacr/make_masks_app_v2.py,sha256=X3izTBXdCZDlkVe-fbG-jmCQtcAbmK0OIivjyWaLhug,30576
+spacr/mask_app.py,sha256=mhTl_XzXLFl8Tx3WYEMpdYB_qw9u5JJa0EdkvlcIzAE,10706
+spacr/measure.py,sha256=0FRsHF5ftar4JZ0B_6Nq-NlyP5t6aiO0IrskyikIBEE,55000
+spacr/measure_app.py,sha256=_C1-XFL5HSquUEEbM_NcxdvHx-socPFCx85MBG4d6xo,10598
+spacr/old_code.py,sha256=jw67DAGoLBd7mWofVzRJSEmCI1Qrff26zIo65SEkV00,13817
+spacr/plot.py,sha256=lrwU51OTWfby1wx73XGyjYmTjLVia7WOmGH5LZZ-4jM,67145
+spacr/sequencing.py,sha256=U_TBJGNfOBfokGegUe950W_KPfm51VOgpfibXoZ8RMQ,83974
+spacr/settings.py,sha256=Tr2fo2I75FGfmEVQOONOpGwqXMzFCrYMz4NAxav3ckg,21183
+spacr/sim.py,sha256=FveaVgBi3eypO2oVB5Dx-v0CC1Ny7UPfXkJiiRRodAk,71212
+spacr/sim_app.py,sha256=47DEQpj8HBSa-_TImW-5JCeuQeRkm5NMpJWZG3hSuFU,0
+spacr/timelapse.py,sha256=KMYCgHzf9LTZe-lWl5mvH2EjbKRE6OhpwdY13wEumGc,39504
+spacr/utils.py,sha256=O7dpCF3bU95d2v0UuPFeJtzXYrkh0r-6aLxaqkKkFwY,184619
+spacr/version.py,sha256=axH5tnGwtgSnJHb5IDhiu4Zjk5GhLyAEDRe-rnaoFOA,409
+spacr/models/cp/toxo_plaque_cyto_e25000_X1120_Y1120.CP_model,sha256=z8BbHWZPRnE9D_BHO0fBREE85c1vkltDs-incs2ytXQ,26566572
+spacr/models/cp/toxo_plaque_cyto_e25000_X1120_Y1120.CP_model_settings.csv,sha256=fBAGuL_B8ERVdVizO3BHozTDSbZUh1yFzsYK3wkQN68,420
+spacr/models/cp/toxo_pv_lumen.CP_model,sha256=2y_CindYhmTvVwBH39SNILF3rI3x9SsRn6qrMxHy3l0,26562451
+spacr-0.1.12.dist-info/LICENSE,sha256=SR-2MeGc6SCM1UORJYyarSWY_A-JaOMFDj7ReSs9tRM,1083
+spacr-0.1.12.dist-info/METADATA,sha256=ppvYUfj-bd4G-fel8rkLsj8tR7J-7Fkh6sQupjufIqQ,5301
+spacr-0.1.12.dist-info/WHEEL,sha256=GJ7t_kWBFywbagK5eo9IoUwLW6oyOeTKmQ-9iHFVNxQ,92
+spacr-0.1.12.dist-info/entry_points.txt,sha256=PxmdWbth1nQzQfE0s2M3XST_of47tBtlBPH20KJrvkE,354
+spacr-0.1.12.dist-info/top_level.txt,sha256=GJPU8FgwRXGzKeut6JopsSRY2R8T3i9lDgya42tLInY,6
+spacr-0.1.12.dist-info/RECORD,,

{spacr-0.1.1.dist-info → spacr-0.1.12.dist-info}/entry_points.txt

@@ -1,9 +1,9 @@
 [console_scripts]
-annotate = spacr.annotate_app:gui_annotation
+annotate = spacr.annotate_app_v2:gui_annotate
 classify = spacr.gui_classify_app:gui_classify
 gui = spacr.gui:gui_app
-gui2 = spacr.gui_2:gui_app
-make_masks = spacr.mask_app:gui_make_masks
+make_masks = spacr.gui_make_mask_app:gui_make_masks
+make_masks2 = spacr.gui_make_mask_app_v2:gui_make_masks
 mask = spacr.gui_mask_app:gui_mask
 measure = spacr.gui_measure_app:gui_measure
 sim = spacr.gui_sim_app:gui_sim

spacr-0.1.1.dist-info/RECORD

@@ -1,40 +0,0 @@
-spacr/__init__.py,sha256=rnb_oYH6HmC1KvJmc7ymrdtHvmMW5t7bn8tJa03cxcA,1286
-spacr/__main__.py,sha256=bkAJJD2kjIqOP-u1kLvct9jQQCeUXzlEjdgitwi1Lm8,75
-spacr/alpha.py,sha256=Y95sLEfpK2OSYKRn3M8eUOU33JJeXfV8zhrC4KnwSTY,35244
-spacr/annotate_app.py,sha256=2X_xnXFN_w19RG99awsTPLzQfQZyQdwbaT-lcRxyV-w,20670
-spacr/annotate_app_v2.py,sha256=kvikj_QbN4EHdyYwB0kjEepEuq2uVwfAF-VJ531qO3Q,22647
-spacr/chris.py,sha256=YlBjSgeZaY8HPy6jkrT_ISAnCMAKVfvCxF0I9eAZLFM,2418
-spacr/cli.py,sha256=507jfOOEV8BoL4eeUcblvH-iiDHdBrEVJLu1ghAAPSc,1800
-spacr/core.py,sha256=m9fsk-qDPow4AzOYpTIsd4jT7PF_L_4y5xillR5eRdk,160253
-spacr/deep_spacr.py,sha256=N0o7ILD2p1FTfU4DFxnpjs00xjLhwib-ev0XGqA6muU,37035
-spacr/foldseek.py,sha256=YIP1d4Ci6CeA9jSyiv-HTDbNmAmcSM9Y_DaOs7wYzLY,33546
-spacr/get_alfafold_structures.py,sha256=ehx_MQgb12k3hFecP6cYVlm5TLO8iWjgevy8ESyS3cw,3544
-spacr/graph_learning.py,sha256=1tR-ZxvXE3dBz1Saw7BeVFcrsUFu9OlUZeZVifih9eo,13070
-spacr/gui.py,sha256=ugBksLGOHdtOLlEuRyyc59TrkYKu3rDf8JxEgiBSVao,6536
-spacr/gui_2.py,sha256=ZAI5quQYbhQJ40vK0NCqU_UMSPLkpfeQpomBWUSM0fc,6946
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