sl-shared-assets 1.0.0rc8__py3-none-any.whl → 1.0.0rc10__py3-none-any.whl
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- sl_shared_assets/__init__.py +0 -36
- sl_shared_assets/ascension_tools.py +279 -0
- sl_shared_assets/cli.py +43 -3
- sl_shared_assets/data_classes.py +178 -144
- sl_shared_assets/packaging_tools.py +1 -0
- {sl_shared_assets-1.0.0rc8.dist-info → sl_shared_assets-1.0.0rc10.dist-info}/METADATA +1 -1
- sl_shared_assets-1.0.0rc10.dist-info/RECORD +14 -0
- {sl_shared_assets-1.0.0rc8.dist-info → sl_shared_assets-1.0.0rc10.dist-info}/entry_points.txt +1 -0
- sl_shared_assets/__init__.pyi +0 -87
- sl_shared_assets/cli.pyi +0 -17
- sl_shared_assets/data_classes.pyi +0 -667
- sl_shared_assets/packaging_tools.pyi +0 -52
- sl_shared_assets/server.pyi +0 -112
- sl_shared_assets/suite2p.pyi +0 -188
- sl_shared_assets/transfer_tools.pyi +0 -53
- sl_shared_assets-1.0.0rc8.dist-info/RECORD +0 -20
- {sl_shared_assets-1.0.0rc8.dist-info → sl_shared_assets-1.0.0rc10.dist-info}/WHEEL +0 -0
- {sl_shared_assets-1.0.0rc8.dist-info → sl_shared_assets-1.0.0rc10.dist-info}/licenses/LICENSE +0 -0
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from pathlib import Path
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def _calculate_file_checksum(base_directory: Path, file_path: Path) -> tuple[str, bytes]:
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"""Calculates xxHash3-128 checksum for a single file and its path relative to the base directory.
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This function is passed to parallel workers used by the calculate_directory_hash() method that iteratively
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calculates the checksum for all files inside a directory. Each call to this function returns the checksum for the
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target file, which includes both the contents of the file and its path relative to the base directory.
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Args:
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base_directory: The path to the base (root) directory which is being checksummed by the main
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'calculate_directory_checksum' function.
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file_path: The absolute path to the target file.
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Returns:
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A tuple with two elements. The first element is the path to the file relative to the base directory. The second
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element is the xxHash3-128 checksum that covers the relative path and the contents of the file.
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"""
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def calculate_directory_checksum(
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directory: Path, num_processes: int | None = None, batch: bool = False, save_checksum: bool = True
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) -> str:
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"""Calculates xxHash3-128 checksum for the input directory, which includes the data of all contained files and
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the directory structure information.
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This function is used to generate a checksum for the raw_data directory of each experiment or training session.
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Checksums are used to verify the session data integrity during transmission between the PC that acquired the data
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and long-term storage locations, such as the Synology NAS or the BioHPC server. The function can be configured to
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write the generated checksum as a hexadecimal string to the ax_checksum.txt file stored at the highest level of the
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input directory.
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Note:
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This method uses multiprocessing to efficiently parallelize checksum calculation for multiple files. In
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combination with xxHash3, this achieves a significant speedup over more common checksums, such as MD5 and
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SHA256. Note that xxHash3 is not suitable for security purposes and is only used to ensure data integrity.
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The method notifies the user about the checksum calculation process via the terminal.
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The returned checksum accounts for both the contents of each file and the layout of the input directory
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structure.
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Args:
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directory: The Path to the directory to be checksummed.
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num_processes: The number of CPU processes to use for parallelizing checksum calculation. If set to None, the
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function defaults to using (logical CPU count - 4).
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batch: Determines whether the function is called as part of batch-processing multiple directories. This is used
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to optimize progress reporting to avoid cluttering the terminal.
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save_checksum: Determines whether the checksum should be saved (written to) a .txt file.
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Returns:
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The xxHash3-128 checksum for the input directory as a hexadecimal string.
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"""
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sl_shared_assets/server.pyi
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from pathlib import Path
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from dataclasses import dataclass
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from simple_slurm import Slurm
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from paramiko.client import SSHClient as SSHClient
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from ataraxis_data_structures import YamlConfig
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def generate_server_credentials(
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output_directory: Path, username: str, password: str, host: str = "cbsuwsun.biohpc.cornell.edu"
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) -> None:
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"""Generates a new server_credentials.yaml file under the specified directory, using input information.
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This function provides a convenience interface for generating new BioHPC server credential files. Generally, this is
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only used when setting up new host-computers in the lab.
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"""
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@dataclass()
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class ServerCredentials(YamlConfig):
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"""This class stores the hostname and credentials used to log into the BioHPC cluster to run Sun lab processing
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pipelines.
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Primarily, this is used as part of the sl-experiment library runtime to start data processing once it is
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transferred to the BioHPC server during preprocessing.
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"""
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username: str = ...
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password: str = ...
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host: str = ...
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class Server:
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"""Encapsulates access to the Sun lab BioHPC processing server.
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This class provides the API that allows accessing the BioHPC server and creating and submitting various
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SLURM-managed jobs to the server. It functions as the central interface used by all processing pipelines in the
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lab to execute costly data processing on the server.
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Notes:
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All lab processing pipelines expect the data to be stored on the server and all processing logic to be packaged
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and installed into dedicated conda environments on the server.
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Args:
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credentials_path: The path to the.yaml file containing the server hostname and access credentials.
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Attributes:
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_open: Tracks whether the connection to the server is open or not.
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_client: Stores the initialized SSHClient instance used to interface with the server.
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"""
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_open: bool
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_credentials: ServerCredentials
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_client: SSHClient
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def __init__(self, credentials_path: Path) -> None: ...
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def __del__(self) -> None:
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"""If the instance is connected to the server, terminates the connection before the instance is destroyed."""
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@staticmethod
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def generate_slurm_header(
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job_name: str, output_log: Path, error_log: Path, cpus_to_use: int = 20, ram_gb: int = 4, time_limit: int = 60
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) -> Slurm:
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"""Creates a SLURM command object and fills it with initial job configuration data.
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This method is used to generate the initial SLURM command object and fill it with job (SLURM) configuration and
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(general!) conda initialization data. It is used by all processing pipelines in the lab as the initial
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configuration point when writing job shell scripts.
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Notes:
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The command header generated by this method does not contain the command to initialize the specific conda
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environment to be used during processing. This has to be provided as part of the additional command
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configuration, typically by adding the "source activate {ENV_NAME}" subcommand to the end of the header
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returned by this method.
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job_name: The descriptive name of the SLURM job to be created.
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output_log: The path to the .txt file on the processing server, where to store the standard output of the
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job.
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error_log: The path to the .txt file on the processing server, where to store the standard error of the
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job.
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cpus_to_use: The number of CPUs to use for the job.
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ram_gb: The amount of RAM to allocate for the job in Gigabytes.
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time_limit: The maximum time limit for the job, in minutes. It is highly advised to set an adequate maximum
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runtime limit to prevent jobs from hogging the server for a long period of time.
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"""
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def submit_job(self, slurm_command: Slurm, working_directory: Path) -> str:
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"""Submits the input SLURM command to the managed BioHPC server via the shell script.
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This method submits various commands for execution via SLURM-managed BioHPC cluster. As part of its runtime, the
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method translates the Slurm object into the shell script, moves the script to the target working directory on
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the server, and instructs the server to execute the shell script (via SLURM).
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Args:
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slurm_command: The Slurm (command) object containing the job configuration and individual commands to run
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as part of the processing pipeline.
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working_directory: The path to the working directory on the server where the shell script is moved
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and executed.
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The job ID assigned to the job by SLURM manager if the command submission is successful.
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Raises:
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RuntimeError: If the command submission to the server fails.
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"""
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def job_complete(self, job_id: str) -> bool:
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"""Returns True if the job with the given ID has been completed or terminated its runtime due to an error.
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If the job is still running or is waiting inside the execution queue, returns False.
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job_id: The numeric ID of the job to check, assigned by SLURM.
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"""
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def close(self) -> None:
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"""Closes the SSH connection to the server.
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This method has to be called before destroying the class instance to ensure proper resource cleanup.
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"""
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sl_shared_assets/suite2p.pyi
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from typing import Any
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from dataclasses import field, dataclass
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from _typeshed import Incomplete
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from ataraxis_data_structures import YamlConfig
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@dataclass
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class Main:
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"""Stores global settings used to broadly define the suite2p processing configuration."""
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nplanes: int = ...
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nchannels: int = ...
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functional_chan: int = ...
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tau: float = ...
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force_sktiff: bool = ...
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fs: float = ...
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do_bidiphase: bool = ...
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bidiphase: int = ...
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bidi_corrected: bool = ...
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frames_include: int = ...
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multiplane_parallel: bool = ...
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ignore_flyback: list[int] = field(default_factory=list)
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@dataclass
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class FileIO:
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"""Stores I/O settings used to specify input data file locations, formats, and output storage options."""
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fast_disk: list[str] = field(default_factory=list)
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delete_bin: bool = ...
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mesoscan: bool = ...
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bruker: bool = ...
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bruker_bidirectional: bool = ...
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h5py: list[str] = field(default_factory=list)
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h5py_key: str = ...
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nwb_file: str = ...
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nwb_driver: str = ...
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nwb_series: str = ...
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save_path0: list[str] = field(default_factory=list)
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save_folder: list[str] = field(default_factory=list)
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look_one_level_down: bool = ...
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subfolders: list[str] = field(default_factory=list)
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move_bin: bool = ...
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class Output:
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"""Stores I/O settings used to define the output format and organization of the processing results."""
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preclassify: float = ...
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save_nwb: bool = ...
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save_mat: bool = ...
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combined: bool = ...
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aspect: float = ...
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report_time: bool = ...
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@dataclass
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class Registration:
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"""Stores rigid registration settings used for correcting motion artifacts between frames."""
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do_registration: bool = ...
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align_by_chan: int = ...
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nimg_init: int = ...
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batch_size: int = ...
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maxregshift: float = ...
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smooth_sigma: float = ...
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smooth_sigma_time: float = ...
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keep_movie_raw: bool = ...
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two_step_registration: bool = ...
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reg_tif: bool = ...
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reg_tif_chan2: bool = ...
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subpixel: int = ...
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th_badframes: float = ...
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norm_frames: bool = ...
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force_refImg: bool = ...
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class OnePRegistration:
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"""Stores additional pre-registration processing settings used to improve the registration of 1-photon datasets."""
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class NonRigid:
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"""Stores non-rigid registration settings used to improve motion registration in complex datasets."""
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block_size: list[int] = field(default_factory=Incomplete)
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class ROIDetection:
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"""Stores ROI detection and extraction settings used to identify cells and their activity signals."""
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threshold_scaling: float = ...
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class CellposeDetection:
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"""Stores Cellpose algorithm settings used for cell detection."""
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cellprob_threshold: float = ...
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flow_threshold: float = ...
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@dataclass
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class SignalExtraction:
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"""Stores settings used to extract fluorescence signals from ROIs and surrounding neuropil regions."""
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neuropil_extract: bool = ...
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allow_overlap: bool = ...
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lam_percentile: int = ...
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@dataclass
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class SpikeDeconvolution:
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"""Stores settings used to deconvolve calcium signals to infer spike trains."""
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neucoeff: float = ...
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baseline: str = ...
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@dataclass
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class Classification:
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"""Stores settings used to classify detected ROIs as real cells or artifacts."""
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classifier_path: str = ...
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@dataclass
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class Channel2:
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"""Stores settings for processing the second channel in multichannel datasets."""
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chan2_thres: float = ...
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@dataclass
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class Suite2PConfiguration(YamlConfig):
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This class is used during processing to instruct suite2p on how to process the data. Specifically, it provides a
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user-friendly way of specifying all user-addressable parameters through a .YAML file. The sl-forgery library then
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loads the data from .yaml file and uses it to configure the single-day suite2p pipeline and the multiday suite2p
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pipeline.
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Notes:
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The .YAML file uses section names that match the suite2p documentation sections. This way, users can always
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consult the suite2p documentation for information on the purpose of each field inside every subsection.
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"""
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main: Main = field(default_factory=Main)
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file_io: FileIO = field(default_factory=FileIO)
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output: Output = field(default_factory=Output)
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registration: Registration = field(default_factory=Registration)
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one_p_registration: OnePRegistration = field(default_factory=OnePRegistration)
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non_rigid: NonRigid = field(default_factory=NonRigid)
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roi_detection: ROIDetection = field(default_factory=ROIDetection)
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cellpose_detection: CellposeDetection = field(default_factory=CellposeDetection)
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signal_extraction: SignalExtraction = field(default_factory=SignalExtraction)
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spike_deconvolution: SpikeDeconvolution = field(default_factory=SpikeDeconvolution)
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classification: Classification = field(default_factory=Classification)
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channel2: Channel2 = field(default_factory=Channel2)
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def to_ops(self) -> dict[str, Any]:
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"""Converts the class instance to a dictionary and returns it to caller.
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This dictionary can be passed to suite2p functions either as an 'ops' or 'db' argument to control the
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processing runtime.
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"""
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from pathlib import Path
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be preserved in the destination directory.
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def transfer_directory(source: Path, destination: Path, num_threads: int = 1, verify_integrity: bool = True) -> None:
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structure.
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This function is used to assemble the experimental data from all remote machines used in the acquisition process on
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the VRPC before the data is preprocessed. It is also used to transfer the preprocessed data from the VRPC to the
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SynologyNAS and the Sun lab BioHPC server.
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Notes:
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This method recreates the moved directory hierarchy on the destination if the hierarchy does not exist. This is
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done before copying the files.
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The method executes a multithreading copy operation. It does not clean up the source files. That job is handed
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to the specific preprocessing function from the sl_experiment or sl-forgery libraries that calls this function.
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If the method is configured to verify transferred file integrity, it reruns the xxHash3-128 checksum calculation
|
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and compares the returned checksum to the one stored in the source directory. The method assumes that all input
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directories contain the 'ax_checksum.txt' file that stores the 'source' directory checksum at the highest level
|
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of the input directory tree.
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-
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-
Args:
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source: The path to the directory that needs to be moved.
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destination: The path to the destination directory where to move the contents of the source directory.
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-
num_threads: The number of threads to use for parallel file transfer. This number should be set depending on the
|
|
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|
-
type of transfer (local or remote) and is not guaranteed to provide improved transfer performance. For local
|
|
45
|
-
transfers, setting this number above 1 will likely provide a performance boost. For remote transfers using
|
|
46
|
-
a single TCP / IP socket (such as non-multichannel SMB protocol), the number should be set to 1.
|
|
47
|
-
verify_integrity: Determines whether to perform integrity verification for the transferred files. Note,
|
|
48
|
-
integrity verification is a time-consuming process and generally would not be a concern for most runtimes.
|
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|
-
Therefore, it is often fine to disable this option to optimize method runtime speed.
|
|
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|
-
|
|
51
|
-
Raises:
|
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|
-
RuntimeError: If the transferred files do not pass the xxHas3-128 checksum integrity verification.
|
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|
-
"""
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|
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sl_shared_assets/__init__.py,sha256=V7EvTTSB_GhetCbyYPg2RoiG1etDVeML5EBWgGvUo7E,2227
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sl_shared_assets/__init__.pyi,sha256=U5Sma4zenITe0-eI_heTUYh_9P0puhgM3hAdcf-qozk,2532
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sl_shared_assets/cli.py,sha256=CjfuXXj7CeDA2pbCwe5Rad6RDjIqGDud14IUDMdzx_w,2639
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sl_shared_assets/cli.pyi,sha256=X5UdXpkzUw71_ftaIXMsnttIeR15SPVLiECuPge_zw8,1032
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sl_shared_assets/data_classes.py,sha256=Gilip0hQJqNS7SJSDK0crXy4eiD6Ws_OO5cBGInqaGw,87076
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sl_shared_assets/data_classes.pyi,sha256=5l2snWnlqTXGiJldGLvI4sc2G2_08aw9bFIOi_RmgjE,32240
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sl_shared_assets/packaging_tools.py,sha256=3kAXFK37Lv4JA1YhjcoBz1x2Ell8ObCqe9pwxAts4m4,6709
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sl_shared_assets/packaging_tools.pyi,sha256=hlAP9AxF7NHtFIPKjj5ehm8Vr9qIn6xDk4VvL0JuAmk,3055
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sl_shared_assets/py.typed,sha256=47DEQpj8HBSa-_TImW-5JCeuQeRkm5NMpJWZG3hSuFU,0
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sl_shared_assets/server.py,sha256=VtFwS4PEy24n_pGz9W56zufkZEf_PKxIllP2ZnF5Zgc,13269
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sl_shared_assets/server.pyi,sha256=Qa-pziy3fAoQKYJn1H4Bh_7oKgJu5ZmWBMogHQLUKZs,5550
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sl_shared_assets/suite2p.py,sha256=sQ5Zj0TJFD-gUHqtWnRvapBpr8QgmaiVil123cWxGxc,20511
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sl_shared_assets/suite2p.pyi,sha256=Uyv8ov--etwJIc6e2UVgs0jYXwnK2CD-kICfXo5KpcI,6331
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|
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sl_shared_assets/transfer_tools.py,sha256=J26kwOp_NpPSY0-xu5FTw9udte-rm_mW1FJyaTNoqQI,6606
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sl_shared_assets/transfer_tools.pyi,sha256=FoH7eYZe7guGHfPr0MK5ggO62uXKwD2aJ7h1Bu7PaEE,3294
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sl_shared_assets-1.0.0rc8.dist-info/METADATA,sha256=6Omp-WLRWXVniIkMLG6SyujEEiJuptEk-2f1SQgA4m0,47806
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17
|
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sl_shared_assets-1.0.0rc8.dist-info/WHEEL,sha256=qtCwoSJWgHk21S1Kb4ihdzI2rlJ1ZKaIurTj_ngOhyQ,87
|
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18
|
-
sl_shared_assets-1.0.0rc8.dist-info/entry_points.txt,sha256=3VPr5RkWBkusNN9OhWXtC-DN0utu7uMrUulazIK2VNA,166
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19
|
-
sl_shared_assets-1.0.0rc8.dist-info/licenses/LICENSE,sha256=OXLcl0T2SZ8Pmy2_dmlvKuetivmyPd5m1q-Gyd-zaYY,35149
|
|
20
|
-
sl_shared_assets-1.0.0rc8.dist-info/RECORD,,
|
|
File without changes
|
{sl_shared_assets-1.0.0rc8.dist-info → sl_shared_assets-1.0.0rc10.dist-info}/licenses/LICENSE
RENAMED
|
File without changes
|