ngio 0.1.6__py3-none-any.whl → 0.2.0__py3-none-any.whl
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- ngio/__init__.py +33 -5
- ngio/common/__init__.py +54 -0
- ngio/common/_array_pipe.py +265 -0
- ngio/common/_axes_transforms.py +64 -0
- ngio/common/_common_types.py +5 -0
- ngio/common/_dimensions.py +120 -0
- ngio/common/_masking_roi.py +158 -0
- ngio/common/_pyramid.py +228 -0
- ngio/common/_roi.py +165 -0
- ngio/common/_slicer.py +96 -0
- ngio/{pipes/_zoom_utils.py → common/_zoom.py} +7 -81
- ngio/hcs/__init__.py +5 -0
- ngio/hcs/plate.py +448 -0
- ngio/images/__init__.py +23 -0
- ngio/images/abstract_image.py +349 -0
- ngio/images/create.py +270 -0
- ngio/images/image.py +453 -0
- ngio/images/label.py +285 -0
- ngio/images/masked_image.py +273 -0
- ngio/images/ome_zarr_container.py +738 -0
- ngio/ome_zarr_meta/__init__.py +47 -0
- ngio/ome_zarr_meta/_meta_handlers.py +791 -0
- ngio/ome_zarr_meta/ngio_specs/__init__.py +71 -0
- ngio/ome_zarr_meta/ngio_specs/_axes.py +481 -0
- ngio/ome_zarr_meta/ngio_specs/_channels.py +389 -0
- ngio/ome_zarr_meta/ngio_specs/_dataset.py +134 -0
- ngio/ome_zarr_meta/ngio_specs/_ngio_hcs.py +377 -0
- ngio/ome_zarr_meta/ngio_specs/_ngio_image.py +489 -0
- ngio/ome_zarr_meta/ngio_specs/_pixel_size.py +116 -0
- ngio/ome_zarr_meta/v04/__init__.py +23 -0
- ngio/ome_zarr_meta/v04/_v04_spec_utils.py +485 -0
- ngio/tables/__init__.py +24 -6
- ngio/tables/_validators.py +190 -0
- ngio/tables/backends/__init__.py +8 -0
- ngio/tables/backends/_abstract_backend.py +71 -0
- ngio/tables/backends/_anndata_utils.py +198 -0
- ngio/tables/backends/_anndata_v1.py +76 -0
- ngio/tables/backends/_json_v1.py +56 -0
- ngio/tables/backends/_table_backends.py +102 -0
- ngio/tables/tables_container.py +310 -0
- ngio/tables/v1/__init__.py +5 -5
- ngio/tables/v1/_feature_table.py +182 -0
- ngio/tables/v1/_generic_table.py +160 -179
- ngio/tables/v1/_roi_table.py +366 -0
- ngio/utils/__init__.py +26 -10
- ngio/utils/_datasets.py +53 -0
- ngio/utils/_errors.py +10 -4
- ngio/utils/_fractal_fsspec_store.py +13 -0
- ngio/utils/_logger.py +3 -1
- ngio/utils/_zarr_utils.py +401 -0
- {ngio-0.1.6.dist-info → ngio-0.2.0.dist-info}/METADATA +30 -43
- ngio-0.2.0.dist-info/RECORD +54 -0
- ngio/core/__init__.py +0 -7
- ngio/core/dimensions.py +0 -122
- ngio/core/image_handler.py +0 -228
- ngio/core/image_like_handler.py +0 -549
- ngio/core/label_handler.py +0 -410
- ngio/core/ngff_image.py +0 -387
- ngio/core/roi.py +0 -92
- ngio/core/utils.py +0 -287
- ngio/io/__init__.py +0 -19
- ngio/io/_zarr.py +0 -88
- ngio/io/_zarr_array_utils.py +0 -0
- ngio/io/_zarr_group_utils.py +0 -60
- ngio/iterators/__init__.py +0 -1
- ngio/ngff_meta/__init__.py +0 -27
- ngio/ngff_meta/fractal_image_meta.py +0 -1267
- ngio/ngff_meta/meta_handler.py +0 -92
- ngio/ngff_meta/utils.py +0 -235
- ngio/ngff_meta/v04/__init__.py +0 -6
- ngio/ngff_meta/v04/specs.py +0 -158
- ngio/ngff_meta/v04/zarr_utils.py +0 -376
- ngio/pipes/__init__.py +0 -7
- ngio/pipes/_slicer_transforms.py +0 -176
- ngio/pipes/_transforms.py +0 -33
- ngio/pipes/data_pipe.py +0 -52
- ngio/tables/_ad_reader.py +0 -80
- ngio/tables/_utils.py +0 -301
- ngio/tables/tables_group.py +0 -252
- ngio/tables/v1/feature_tables.py +0 -182
- ngio/tables/v1/masking_roi_tables.py +0 -243
- ngio/tables/v1/roi_tables.py +0 -285
- ngio/utils/_common_types.py +0 -5
- ngio/utils/_pydantic_utils.py +0 -52
- ngio-0.1.6.dist-info/RECORD +0 -44
- {ngio-0.1.6.dist-info → ngio-0.2.0.dist-info}/WHEEL +0 -0
- {ngio-0.1.6.dist-info → ngio-0.2.0.dist-info}/licenses/LICENSE +0 -0
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"""Abstract class for handling OME-NGFF images."""
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from collections.abc import Collection
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from typing import Literal, overload
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import numpy as np
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from ngio.images.create import _create_empty_image
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from ngio.images.image import Image, ImagesContainer
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from ngio.images.label import Label, LabelsContainer
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from ngio.images.masked_image import MaskedImage, MaskedLabel
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from ngio.ome_zarr_meta import (
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NgioImageMeta,
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PixelSize,
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)
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from ngio.ome_zarr_meta.ngio_specs import (
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SpaceUnits,
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TimeUnits,
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)
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from ngio.tables import (
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FeatureTable,
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GenericRoiTable,
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MaskingRoiTable,
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RoiTable,
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Table,
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TablesContainer,
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TypedTable,
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)
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from ngio.utils import (
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AccessModeLiteral,
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NgioValidationError,
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NgioValueError,
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StoreOrGroup,
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ZarrGroupHandler,
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)
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def _default_table_container(handler: ZarrGroupHandler) -> TablesContainer | None:
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"""Return a default table container."""
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success, table_handler = handler.safe_derive_handler("tables")
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if success and isinstance(table_handler, ZarrGroupHandler):
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return TablesContainer(table_handler)
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def _default_label_container(handler: ZarrGroupHandler) -> LabelsContainer | None:
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"""Return a default label container."""
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success, label_handler = handler.safe_derive_handler("labels")
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if success and isinstance(label_handler, ZarrGroupHandler):
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return LabelsContainer(label_handler)
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class OmeZarrContainer:
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"""This class contains an OME-Zarr image and its associated tables and labels."""
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_images_container: ImagesContainer
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_labels_container: LabelsContainer | None
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_tables_container: TablesContainer | None
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def __init__(
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self,
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group_handler: ZarrGroupHandler,
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table_container: TablesContainer | None = None,
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label_container: LabelsContainer | None = None,
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validate_arrays: bool = True,
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) -> None:
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"""Initialize the OmeZarrContainer."""
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self._group_handler = group_handler
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self._images_container = ImagesContainer(self._group_handler)
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self._labels_container = label_container
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self._tables_container = table_container
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def __repr__(self) -> str:
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"""Return a string representation of the image."""
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num_labels = len(self.list_labels())
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num_tables = len(self.list_tables())
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base_str = f"OmeZarrContainer(levels={self.levels}"
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if num_labels > 0 and num_labels < 3:
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base_str += f", labels={self.list_labels()}"
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elif num_labels >= 3:
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base_str += f", #labels={num_labels}"
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if num_tables > 0 and num_tables < 3:
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base_str += f", tables={self.list_tables()}"
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elif num_tables >= 3:
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base_str += f", #tables={num_tables}"
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base_str += ")"
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return base_str
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@property
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def images_container(self) -> ImagesContainer:
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"""Return the image container."""
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return self._images_container
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@property
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def labels_container(self) -> LabelsContainer:
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"""Return the labels container."""
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if self._labels_container is None:
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self._labels_container = _default_label_container(self._group_handler)
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if self._labels_container is None:
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raise NgioValidationError("No labels found in the image.")
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return self._labels_container
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@property
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def tables_container(self) -> TablesContainer:
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"""Return the tables container."""
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if self._tables_container is None:
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self._tables_container = _default_table_container(self._group_handler)
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if self._tables_container is None:
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raise NgioValidationError("No tables found in the image.")
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return self._tables_container
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@property
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def image_meta(self) -> NgioImageMeta:
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"""Return the image metadata."""
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return self._images_container.meta
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@property
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def levels(self) -> int:
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"""Return the number of levels in the image."""
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return self._images_container.levels
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@property
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def levels_paths(self) -> list[str]:
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"""Return the paths of the levels in the image."""
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return self._images_container.levels_paths
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@property
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def is_3d(self) -> bool:
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"""Return True if the image is 3D."""
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return self.get_image().is_3d
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@property
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def is_2d(self) -> bool:
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"""Return True if the image is 2D."""
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return self.get_image().is_2d
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@property
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def is_time_series(self) -> bool:
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"""Return True if the image is a time series."""
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return self.get_image().is_time_series
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@property
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def is_2d_time_series(self) -> bool:
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"""Return True if the image is a 2D time series."""
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return self.get_image().is_2d_time_series
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@property
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def is_3d_time_series(self) -> bool:
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"""Return True if the image is a 3D time series."""
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return self.get_image().is_3d_time_series
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@property
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def is_multi_channels(self) -> bool:
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"""Return True if the image is multichannel."""
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return self.get_image().is_multi_channels
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def initialize_channel_meta(
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self,
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labels: Collection[str] | int | None = None,
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wavelength_id: Collection[str] | None = None,
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percentiles: tuple[float, float] | None = None,
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colors: Collection[str] | None = None,
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active: Collection[bool] | None = None,
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**omero_kwargs: dict,
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) -> None:
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"""Create a ChannelsMeta object with the default unit."""
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self._images_container.initialize_channel_meta(
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labels=labels,
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wavelength_id=wavelength_id,
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percentiles=percentiles,
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colors=colors,
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active=active,
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**omero_kwargs,
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)
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def update_percentiles(
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self,
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start_percentile: float = 0.1,
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end_percentile: float = 99.9,
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) -> None:
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"""Update the percentiles of the image."""
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self._images_container.update_percentiles(
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start_percentile=start_percentile, end_percentile=end_percentile
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)
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def get_image(
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self,
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path: str | None = None,
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pixel_size: PixelSize | None = None,
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strict: bool = False,
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) -> Image:
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"""Get an image at a specific level.
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Args:
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path (str | None): The path to the image in the ome_zarr file.
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pixel_size (PixelSize | None): The pixel size of the image.
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strict (bool): Only used if the pixel size is provided. If True, the
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pixel size must match the image pixel size exactly. If False, the
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closest pixel size level will be returned.
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"""
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return self._images_container.get(
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path=path, pixel_size=pixel_size, strict=strict
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)
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def get_masked_image(
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self,
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masking_label_name: str,
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masking_table_name: str | None = None,
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path: str | None = None,
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pixel_size: PixelSize | None = None,
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strict: bool = False,
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) -> MaskedImage:
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"""Get a masked image at a specific level.
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Args:
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masking_label_name (str): The name of the label.
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masking_table_name (str | None): The name of the masking table.
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path (str | None): The path to the image in the ome_zarr file.
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pixel_size (PixelSize | None): The pixel size of the image.
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strict (bool): Only used if the pixel size is provided. If True, the
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pixel size must match the image pixel size exactly. If False, the
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closest pixel size level will be returned.
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"""
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image = self.get_image(path=path, pixel_size=pixel_size, strict=strict)
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masking_label = self.get_label(
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name=masking_label_name, path=path, pixel_size=pixel_size, strict=strict
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)
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if masking_table_name is None:
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masking_table = masking_label.build_masking_roi_table()
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else:
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masking_table = self.get_table(
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masking_table_name, check_type="masking_roi_table"
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)
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return MaskedImage(
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group_handler=image._group_handler,
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path=masking_label.path,
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meta_handler=image.meta_handler,
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label=masking_label,
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masking_roi_table=masking_table,
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)
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def derive_image(
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self,
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store: StoreOrGroup,
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ref_path: str | None = None,
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shape: Collection[int] | None = None,
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labels: Collection[str] | None = None,
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pixel_size: PixelSize | None = None,
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axes_names: Collection[str] | None = None,
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chunks: Collection[int] | None = None,
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dtype: str | None = None,
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copy_labels: bool = False,
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copy_tables: bool = False,
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overwrite: bool = False,
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) -> "OmeZarrContainer":
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"""Create an empty OME-Zarr container from an existing image.
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260
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+
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261
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+
Args:
|
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262
|
+
store (StoreOrGroup): The Zarr store or group to create the image in.
|
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263
|
+
ref_path (str | None): The path to the reference image in
|
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264
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+
the image container.
|
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265
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+
shape (Collection[int] | None): The shape of the new image.
|
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266
|
+
labels (Collection[str] | None): The labels of the new image.
|
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267
|
+
pixel_size (PixelSize | None): The pixel size of the new image.
|
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268
|
+
axes_names (Collection[str] | None): The axes names of the new image.
|
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269
|
+
chunks (Collection[int] | None): The chunk shape of the new image.
|
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270
|
+
dtype (str | None): The data type of the new image.
|
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271
|
+
copy_labels (bool): Whether to copy the labels from the reference image.
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+
copy_tables (bool): Whether to copy the tables from the reference image.
|
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|
+
overwrite (bool): Whether to overwrite an existing image.
|
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|
+
|
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+
Returns:
|
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|
+
OmeZarrContainer: The new image container.
|
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|
+
|
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|
+
"""
|
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279
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+
_ = self._images_container.derive(
|
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+
store=store,
|
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281
|
+
ref_path=ref_path,
|
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282
|
+
shape=shape,
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283
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+
labels=labels,
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+
pixel_size=pixel_size,
|
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285
|
+
axes_names=axes_names,
|
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286
|
+
chunks=chunks,
|
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287
|
+
dtype=dtype,
|
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288
|
+
overwrite=overwrite,
|
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289
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+
)
|
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290
|
+
|
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291
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+
handler = ZarrGroupHandler(
|
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+
store, cache=self._group_handler.use_cache, mode=self._group_handler.mode
|
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293
|
+
)
|
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294
|
+
|
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295
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+
new_ome_zarr = OmeZarrContainer(
|
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+
group_handler=handler,
|
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297
|
+
validate_arrays=False,
|
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298
|
+
)
|
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299
|
+
|
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300
|
+
if copy_labels:
|
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301
|
+
self.labels_container._group_handler.copy_handler(
|
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302
|
+
new_ome_zarr.labels_container._group_handler
|
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303
|
+
)
|
|
304
|
+
|
|
305
|
+
if copy_tables:
|
|
306
|
+
self.tables_container._group_handler.copy_handler(
|
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307
|
+
new_ome_zarr.tables_container._group_handler
|
|
308
|
+
)
|
|
309
|
+
return new_ome_zarr
|
|
310
|
+
|
|
311
|
+
def list_tables(self) -> list[str]:
|
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312
|
+
"""List all tables in the image."""
|
|
313
|
+
return self.tables_container.list()
|
|
314
|
+
|
|
315
|
+
def list_roi_tables(self) -> list[str]:
|
|
316
|
+
"""List all ROI tables in the image."""
|
|
317
|
+
return self.tables_container.list_roi_tables()
|
|
318
|
+
|
|
319
|
+
@overload
|
|
320
|
+
def get_table(self, name: str, check_type: None) -> Table: ...
|
|
321
|
+
|
|
322
|
+
@overload
|
|
323
|
+
def get_table(self, name: str, check_type: Literal["roi_table"]) -> RoiTable: ...
|
|
324
|
+
|
|
325
|
+
@overload
|
|
326
|
+
def get_table(
|
|
327
|
+
self, name: str, check_type: Literal["masking_roi_table"]
|
|
328
|
+
) -> MaskingRoiTable: ...
|
|
329
|
+
|
|
330
|
+
@overload
|
|
331
|
+
def get_table(
|
|
332
|
+
self, name: str, check_type: Literal["feature_table"]
|
|
333
|
+
) -> FeatureTable: ...
|
|
334
|
+
|
|
335
|
+
@overload
|
|
336
|
+
def get_table(
|
|
337
|
+
self, name: str, check_type: Literal["generic_roi_table"]
|
|
338
|
+
) -> GenericRoiTable: ...
|
|
339
|
+
|
|
340
|
+
def get_table(self, name: str, check_type: TypedTable | None = None) -> Table:
|
|
341
|
+
"""Get a table from the image."""
|
|
342
|
+
table = self.tables_container.get(name)
|
|
343
|
+
match check_type:
|
|
344
|
+
case "roi_table":
|
|
345
|
+
if not isinstance(table, RoiTable):
|
|
346
|
+
raise NgioValueError(
|
|
347
|
+
f"Table '{name}' is not a ROI table. Found type: {table.type()}"
|
|
348
|
+
)
|
|
349
|
+
return table
|
|
350
|
+
case "masking_roi_table":
|
|
351
|
+
if not isinstance(table, MaskingRoiTable):
|
|
352
|
+
raise NgioValueError(
|
|
353
|
+
f"Table '{name}' is not a masking ROI table. "
|
|
354
|
+
f"Found type: {table.type()}"
|
|
355
|
+
)
|
|
356
|
+
return table
|
|
357
|
+
|
|
358
|
+
case "generic_roi_table":
|
|
359
|
+
if not isinstance(table, GenericRoiTable):
|
|
360
|
+
raise NgioValueError(
|
|
361
|
+
f"Table '{name}' is not a generic ROI table. "
|
|
362
|
+
f"Found type: {table.type()}"
|
|
363
|
+
)
|
|
364
|
+
return table
|
|
365
|
+
|
|
366
|
+
case "feature_table":
|
|
367
|
+
if not isinstance(table, FeatureTable):
|
|
368
|
+
raise NgioValueError(
|
|
369
|
+
f"Table '{name}' is not a feature table. "
|
|
370
|
+
f"Found type: {table.type()}"
|
|
371
|
+
)
|
|
372
|
+
return table
|
|
373
|
+
case None:
|
|
374
|
+
return table
|
|
375
|
+
case _:
|
|
376
|
+
raise NgioValueError(f"Unknown check_type: {check_type}")
|
|
377
|
+
|
|
378
|
+
def build_image_roi_table(self, name: str = "image") -> RoiTable:
|
|
379
|
+
"""Compute the ROI table for an image."""
|
|
380
|
+
return self.get_image().build_image_roi_table(name=name)
|
|
381
|
+
|
|
382
|
+
def build_masking_roi_table(self, label: str) -> MaskingRoiTable:
|
|
383
|
+
"""Compute the masking ROI table for a label."""
|
|
384
|
+
return self.get_label(label).build_masking_roi_table()
|
|
385
|
+
|
|
386
|
+
def add_table(
|
|
387
|
+
self,
|
|
388
|
+
name: str,
|
|
389
|
+
table: Table,
|
|
390
|
+
backend: str | None = None,
|
|
391
|
+
overwrite: bool = False,
|
|
392
|
+
) -> None:
|
|
393
|
+
"""Add a table to the image."""
|
|
394
|
+
self.tables_container.add(
|
|
395
|
+
name=name, table=table, backend=backend, overwrite=overwrite
|
|
396
|
+
)
|
|
397
|
+
|
|
398
|
+
def list_labels(self) -> list[str]:
|
|
399
|
+
"""List all labels in the image."""
|
|
400
|
+
return self.labels_container.list()
|
|
401
|
+
|
|
402
|
+
def get_label(
|
|
403
|
+
self,
|
|
404
|
+
name: str,
|
|
405
|
+
path: str | None = None,
|
|
406
|
+
pixel_size: PixelSize | None = None,
|
|
407
|
+
strict: bool = False,
|
|
408
|
+
) -> Label:
|
|
409
|
+
"""Get a label from the group.
|
|
410
|
+
|
|
411
|
+
Args:
|
|
412
|
+
name (str): The name of the label.
|
|
413
|
+
path (str | None): The path to the image in the ome_zarr file.
|
|
414
|
+
pixel_size (PixelSize | None): The pixel size of the image.
|
|
415
|
+
strict (bool): Only used if the pixel size is provided. If True, the
|
|
416
|
+
pixel size must match the image pixel size exactly. If False, the
|
|
417
|
+
closest pixel size level will be returned.
|
|
418
|
+
"""
|
|
419
|
+
return self.labels_container.get(
|
|
420
|
+
name=name, path=path, pixel_size=pixel_size, strict=strict
|
|
421
|
+
)
|
|
422
|
+
|
|
423
|
+
def get_masked_label(
|
|
424
|
+
self,
|
|
425
|
+
label_name: str,
|
|
426
|
+
masking_label_name: str,
|
|
427
|
+
masking_table_name: str | None = None,
|
|
428
|
+
path: str | None = None,
|
|
429
|
+
pixel_size: PixelSize | None = None,
|
|
430
|
+
strict: bool = False,
|
|
431
|
+
) -> MaskedLabel:
|
|
432
|
+
"""Get a masked image at a specific level.
|
|
433
|
+
|
|
434
|
+
Args:
|
|
435
|
+
label_name (str): The name of the label.
|
|
436
|
+
masking_label_name (str): The name of the masking label.
|
|
437
|
+
masking_table_name (str | None): The name of the masking table.
|
|
438
|
+
path (str | None): The path to the image in the ome_zarr file.
|
|
439
|
+
pixel_size (PixelSize | None): The pixel size of the image.
|
|
440
|
+
strict (bool): Only used if the pixel size is provided. If True, the
|
|
441
|
+
pixel size must match the image pixel size exactly. If False, the
|
|
442
|
+
closest pixel size level will be returned.
|
|
443
|
+
"""
|
|
444
|
+
label = self.get_label(
|
|
445
|
+
name=label_name, path=path, pixel_size=pixel_size, strict=strict
|
|
446
|
+
)
|
|
447
|
+
masking_label = self.get_label(
|
|
448
|
+
name=masking_label_name, path=path, pixel_size=pixel_size, strict=strict
|
|
449
|
+
)
|
|
450
|
+
if masking_table_name is None:
|
|
451
|
+
masking_table = masking_label.build_masking_roi_table()
|
|
452
|
+
else:
|
|
453
|
+
masking_table = self.get_table(
|
|
454
|
+
masking_table_name, check_type="masking_roi_table"
|
|
455
|
+
)
|
|
456
|
+
|
|
457
|
+
return MaskedLabel(
|
|
458
|
+
group_handler=label._group_handler,
|
|
459
|
+
path=label.path,
|
|
460
|
+
meta_handler=label.meta_handler,
|
|
461
|
+
label=masking_label,
|
|
462
|
+
masking_roi_table=masking_table,
|
|
463
|
+
)
|
|
464
|
+
|
|
465
|
+
def derive_label(
|
|
466
|
+
self,
|
|
467
|
+
name: str,
|
|
468
|
+
ref_image: Image | None = None,
|
|
469
|
+
shape: Collection[int] | None = None,
|
|
470
|
+
pixel_size: PixelSize | None = None,
|
|
471
|
+
axes_names: Collection[str] | None = None,
|
|
472
|
+
chunks: Collection[int] | None = None,
|
|
473
|
+
dtype: str | None = None,
|
|
474
|
+
overwrite: bool = False,
|
|
475
|
+
) -> "Label":
|
|
476
|
+
"""Create an empty OME-Zarr label from a reference image.
|
|
477
|
+
|
|
478
|
+
And add the label to the /labels group.
|
|
479
|
+
|
|
480
|
+
Args:
|
|
481
|
+
store (StoreOrGroup): The Zarr store or group to create the image in.
|
|
482
|
+
ref_image (Image): The reference image.
|
|
483
|
+
name (str): The name of the new image.
|
|
484
|
+
shape (Collection[int] | None): The shape of the new image.
|
|
485
|
+
pixel_size (PixelSize | None): The pixel size of the new image.
|
|
486
|
+
axes_names (Collection[str] | None): The axes names of the new image.
|
|
487
|
+
For labels, the channel axis is not allowed.
|
|
488
|
+
chunks (Collection[int] | None): The chunk shape of the new image.
|
|
489
|
+
dtype (str | None): The data type of the new image.
|
|
490
|
+
overwrite (bool): Whether to overwrite an existing image.
|
|
491
|
+
|
|
492
|
+
Returns:
|
|
493
|
+
Label: The new label.
|
|
494
|
+
|
|
495
|
+
"""
|
|
496
|
+
if ref_image is None:
|
|
497
|
+
ref_image = self.get_image()
|
|
498
|
+
return self.labels_container.derive(
|
|
499
|
+
name=name,
|
|
500
|
+
ref_image=ref_image,
|
|
501
|
+
shape=shape,
|
|
502
|
+
pixel_size=pixel_size,
|
|
503
|
+
axes_names=axes_names,
|
|
504
|
+
chunks=chunks,
|
|
505
|
+
dtype=dtype,
|
|
506
|
+
overwrite=overwrite,
|
|
507
|
+
)
|
|
508
|
+
|
|
509
|
+
|
|
510
|
+
def open_ome_zarr_container(
|
|
511
|
+
store: StoreOrGroup,
|
|
512
|
+
cache: bool = False,
|
|
513
|
+
mode: AccessModeLiteral = "r+",
|
|
514
|
+
validate_arrays: bool = True,
|
|
515
|
+
) -> OmeZarrContainer:
|
|
516
|
+
"""Open an OME-Zarr image."""
|
|
517
|
+
handler = ZarrGroupHandler(store=store, cache=cache, mode=mode)
|
|
518
|
+
return OmeZarrContainer(
|
|
519
|
+
group_handler=handler,
|
|
520
|
+
validate_arrays=validate_arrays,
|
|
521
|
+
)
|
|
522
|
+
|
|
523
|
+
|
|
524
|
+
def open_image(
|
|
525
|
+
store: StoreOrGroup,
|
|
526
|
+
path: str | None = None,
|
|
527
|
+
pixel_size: PixelSize | None = None,
|
|
528
|
+
strict: bool = True,
|
|
529
|
+
cache: bool = False,
|
|
530
|
+
mode: AccessModeLiteral = "r+",
|
|
531
|
+
) -> Image:
|
|
532
|
+
"""Open a single level image from an OME-Zarr image.
|
|
533
|
+
|
|
534
|
+
Args:
|
|
535
|
+
store (StoreOrGroup): The Zarr store or group to create the image in.
|
|
536
|
+
path (str | None): The path to the image in the ome_zarr file.
|
|
537
|
+
pixel_size (PixelSize | None): The pixel size of the image.
|
|
538
|
+
strict (bool): Only used if the pixel size is provided. If True, the
|
|
539
|
+
pixel size must match the image pixel size exactly. If False, the
|
|
540
|
+
closest pixel size level will be returned.
|
|
541
|
+
cache (bool): Whether to use a cache for the zarr group metadata.
|
|
542
|
+
mode (AccessModeLiteral): The
|
|
543
|
+
access mode for the image. Defaults to "r+".
|
|
544
|
+
"""
|
|
545
|
+
group_handler = ZarrGroupHandler(store, cache, mode)
|
|
546
|
+
images_container = ImagesContainer(group_handler)
|
|
547
|
+
return images_container.get(
|
|
548
|
+
path=path,
|
|
549
|
+
pixel_size=pixel_size,
|
|
550
|
+
strict=strict,
|
|
551
|
+
)
|
|
552
|
+
|
|
553
|
+
|
|
554
|
+
def create_empty_ome_zarr(
|
|
555
|
+
store: StoreOrGroup,
|
|
556
|
+
shape: Collection[int],
|
|
557
|
+
xy_pixelsize: float,
|
|
558
|
+
z_spacing: float = 1.0,
|
|
559
|
+
time_spacing: float = 1.0,
|
|
560
|
+
levels: int | list[str] = 5,
|
|
561
|
+
xy_scaling_factor: float = 2,
|
|
562
|
+
z_scaling_factor: float = 1.0,
|
|
563
|
+
space_unit: SpaceUnits | str | None = None,
|
|
564
|
+
time_unit: TimeUnits | str | None = None,
|
|
565
|
+
axes_names: Collection[str] | None = None,
|
|
566
|
+
name: str | None = None,
|
|
567
|
+
chunks: Collection[int] | None = None,
|
|
568
|
+
dtype: str = "uint16",
|
|
569
|
+
channel_labels: list[str] | None = None,
|
|
570
|
+
channel_wavelengths: list[str] | None = None,
|
|
571
|
+
percentiles: tuple[float, float] | None = None,
|
|
572
|
+
channel_colors: Collection[str] | None = None,
|
|
573
|
+
channel_active: Collection[bool] | None = None,
|
|
574
|
+
overwrite: bool = False,
|
|
575
|
+
version: str = "0.4",
|
|
576
|
+
) -> OmeZarrContainer:
|
|
577
|
+
"""Create an empty OME-Zarr image with the given shape and metadata.
|
|
578
|
+
|
|
579
|
+
Args:
|
|
580
|
+
store (StoreOrGroup): The Zarr store or group to create the image in.
|
|
581
|
+
shape (Collection[int]): The shape of the image.
|
|
582
|
+
xy_pixelsize (float): The pixel size in x and y dimensions.
|
|
583
|
+
z_spacing (float, optional): The spacing between z slices. Defaults to 1.0.
|
|
584
|
+
time_spacing (float, optional): The spacing between time points.
|
|
585
|
+
Defaults to 1.0.
|
|
586
|
+
levels (int | list[str], optional): The number of levels in the pyramid or a
|
|
587
|
+
list of level names. Defaults to 5.
|
|
588
|
+
xy_scaling_factor (float, optional): The down-scaling factor in x and y
|
|
589
|
+
dimensions. Defaults to 2.0.
|
|
590
|
+
z_scaling_factor (float, optional): The down-scaling factor in z dimension.
|
|
591
|
+
Defaults to 1.0.
|
|
592
|
+
space_unit (SpaceUnits | str | None, optional): The unit of space. Defaults to
|
|
593
|
+
None.
|
|
594
|
+
time_unit (TimeUnits | str | None, optional): The unit of time. Defaults to
|
|
595
|
+
None.
|
|
596
|
+
axes_names (Collection[str] | None, optional): The names of the axes.
|
|
597
|
+
If None the canonical names are used. Defaults to None.
|
|
598
|
+
name (str | None, optional): The name of the image. Defaults to None.
|
|
599
|
+
chunks (Collection[int] | None, optional): The chunk shape. If None the shape
|
|
600
|
+
is used. Defaults to None.
|
|
601
|
+
dtype (str, optional): The data type of the image. Defaults to "uint16".
|
|
602
|
+
channel_labels (list[str] | None, optional): The labels of the channels.
|
|
603
|
+
Defaults to None.
|
|
604
|
+
channel_wavelengths (list[str] | None, optional): The wavelengths of the
|
|
605
|
+
channels. Defaults to None.
|
|
606
|
+
percentiles (tuple[float, float] | None, optional): The percentiles of the
|
|
607
|
+
channels. Defaults to None.
|
|
608
|
+
channel_colors (Collection[str] | None, optional): The colors of the channels.
|
|
609
|
+
Defaults to None.
|
|
610
|
+
channel_active (Collection[bool] | None, optional): Whether the channels are
|
|
611
|
+
active. Defaults to None.
|
|
612
|
+
overwrite (bool, optional): Whether to overwrite an existing image.
|
|
613
|
+
Defaults to True.
|
|
614
|
+
version (str, optional): The version of the OME-Zarr specification.
|
|
615
|
+
Defaults to "0.4".
|
|
616
|
+
"""
|
|
617
|
+
handler = _create_empty_image(
|
|
618
|
+
store=store,
|
|
619
|
+
shape=shape,
|
|
620
|
+
pixelsize=xy_pixelsize,
|
|
621
|
+
z_spacing=z_spacing,
|
|
622
|
+
time_spacing=time_spacing,
|
|
623
|
+
levels=levels,
|
|
624
|
+
yx_scaling_factor=xy_scaling_factor,
|
|
625
|
+
z_scaling_factor=z_scaling_factor,
|
|
626
|
+
space_unit=space_unit,
|
|
627
|
+
time_unit=time_unit,
|
|
628
|
+
axes_names=axes_names,
|
|
629
|
+
name=name,
|
|
630
|
+
chunks=chunks,
|
|
631
|
+
dtype=dtype,
|
|
632
|
+
overwrite=overwrite,
|
|
633
|
+
version=version,
|
|
634
|
+
)
|
|
635
|
+
|
|
636
|
+
ome_zarr = OmeZarrContainer(group_handler=handler)
|
|
637
|
+
ome_zarr.initialize_channel_meta(
|
|
638
|
+
labels=channel_labels,
|
|
639
|
+
wavelength_id=channel_wavelengths,
|
|
640
|
+
percentiles=percentiles,
|
|
641
|
+
colors=channel_colors,
|
|
642
|
+
active=channel_active,
|
|
643
|
+
)
|
|
644
|
+
return ome_zarr
|
|
645
|
+
|
|
646
|
+
|
|
647
|
+
def create_ome_zarr_from_array(
|
|
648
|
+
store: StoreOrGroup,
|
|
649
|
+
array: np.ndarray,
|
|
650
|
+
xy_pixelsize: float,
|
|
651
|
+
z_spacing: float = 1.0,
|
|
652
|
+
time_spacing: float = 1.0,
|
|
653
|
+
levels: int | list[str] = 5,
|
|
654
|
+
xy_scaling_factor: float = 2.0,
|
|
655
|
+
z_scaling_factor: float = 1.0,
|
|
656
|
+
space_unit: SpaceUnits | str | None = None,
|
|
657
|
+
time_unit: TimeUnits | str | None = None,
|
|
658
|
+
axes_names: Collection[str] | None = None,
|
|
659
|
+
channel_labels: list[str] | None = None,
|
|
660
|
+
channel_wavelengths: list[str] | None = None,
|
|
661
|
+
percentiles: tuple[float, float] | None = (0.1, 99.9),
|
|
662
|
+
channel_colors: Collection[str] | None = None,
|
|
663
|
+
channel_active: Collection[bool] | None = None,
|
|
664
|
+
name: str | None = None,
|
|
665
|
+
chunks: Collection[int] | None = None,
|
|
666
|
+
overwrite: bool = False,
|
|
667
|
+
version: str = "0.4",
|
|
668
|
+
) -> OmeZarrContainer:
|
|
669
|
+
"""Create an OME-Zarr image from a numpy array.
|
|
670
|
+
|
|
671
|
+
Args:
|
|
672
|
+
store (StoreOrGroup): The Zarr store or group to create the image in.
|
|
673
|
+
array (np.ndarray): The image data.
|
|
674
|
+
xy_pixelsize (float): The pixel size in x and y dimensions.
|
|
675
|
+
z_spacing (float, optional): The spacing between z slices. Defaults to 1.0.
|
|
676
|
+
time_spacing (float, optional): The spacing between time points.
|
|
677
|
+
Defaults to 1.0.
|
|
678
|
+
levels (int | list[str], optional): The number of levels in the pyramid or a
|
|
679
|
+
list of level names. Defaults to 5.
|
|
680
|
+
xy_scaling_factor (float, optional): The down-scaling factor in x and y
|
|
681
|
+
dimensions. Defaults to 2.0.
|
|
682
|
+
z_scaling_factor (float, optional): The down-scaling factor in z dimension.
|
|
683
|
+
Defaults to 1.0.
|
|
684
|
+
space_unit (SpaceUnits | str | None, optional): The unit of space. Defaults to
|
|
685
|
+
None.
|
|
686
|
+
time_unit (TimeUnits | str | None, optional): The unit of time. Defaults to
|
|
687
|
+
None.
|
|
688
|
+
axes_names (Collection[str] | None, optional): The names of the axes.
|
|
689
|
+
If None the canonical names are used. Defaults to None.
|
|
690
|
+
name (str | None, optional): The name of the image. Defaults to None.
|
|
691
|
+
chunks (Collection[int] | None, optional): The chunk shape. If None the shape
|
|
692
|
+
is used. Defaults to None.
|
|
693
|
+
channel_labels (list[str] | None, optional): The labels of the channels.
|
|
694
|
+
Defaults to None.
|
|
695
|
+
channel_wavelengths (list[str] | None, optional): The wavelengths of the
|
|
696
|
+
channels. Defaults to None.
|
|
697
|
+
percentiles (tuple[float, float] | None, optional): The percentiles of the
|
|
698
|
+
channels. Defaults to None.
|
|
699
|
+
channel_colors (Collection[str] | None, optional): The colors of the channels.
|
|
700
|
+
Defaults to None.
|
|
701
|
+
channel_active (Collection[bool] | None, optional): Whether the channels are
|
|
702
|
+
active. Defaults to None.
|
|
703
|
+
overwrite (bool, optional): Whether to overwrite an existing image.
|
|
704
|
+
Defaults to True.
|
|
705
|
+
version (str, optional): The version of the OME-Zarr specification.
|
|
706
|
+
Defaults to "0.4".
|
|
707
|
+
"""
|
|
708
|
+
handler = _create_empty_image(
|
|
709
|
+
store=store,
|
|
710
|
+
shape=array.shape,
|
|
711
|
+
pixelsize=xy_pixelsize,
|
|
712
|
+
z_spacing=z_spacing,
|
|
713
|
+
time_spacing=time_spacing,
|
|
714
|
+
levels=levels,
|
|
715
|
+
yx_scaling_factor=xy_scaling_factor,
|
|
716
|
+
z_scaling_factor=z_scaling_factor,
|
|
717
|
+
space_unit=space_unit,
|
|
718
|
+
time_unit=time_unit,
|
|
719
|
+
axes_names=axes_names,
|
|
720
|
+
name=name,
|
|
721
|
+
chunks=chunks,
|
|
722
|
+
dtype=array.dtype,
|
|
723
|
+
overwrite=overwrite,
|
|
724
|
+
version=version,
|
|
725
|
+
)
|
|
726
|
+
|
|
727
|
+
ome_zarr = OmeZarrContainer(group_handler=handler)
|
|
728
|
+
image = ome_zarr.get_image()
|
|
729
|
+
image.set_array(array)
|
|
730
|
+
image.consolidate()
|
|
731
|
+
ome_zarr.initialize_channel_meta(
|
|
732
|
+
labels=channel_labels,
|
|
733
|
+
wavelength_id=channel_wavelengths,
|
|
734
|
+
percentiles=percentiles,
|
|
735
|
+
colors=channel_colors,
|
|
736
|
+
active=channel_active,
|
|
737
|
+
)
|
|
738
|
+
return ome_zarr
|