napari-tmidas 0.2.2__py3-none-any.whl → 0.2.5__py3-none-any.whl
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- napari_tmidas/__init__.py +35 -5
- napari_tmidas/_crop_anything.py +1520 -609
- napari_tmidas/_env_manager.py +76 -0
- napari_tmidas/_file_conversion.py +1646 -1131
- napari_tmidas/_file_selector.py +1455 -216
- napari_tmidas/_label_inspection.py +83 -8
- napari_tmidas/_processing_worker.py +309 -0
- napari_tmidas/_reader.py +6 -10
- napari_tmidas/_registry.py +2 -2
- napari_tmidas/_roi_colocalization.py +1221 -84
- napari_tmidas/_tests/test_crop_anything.py +123 -0
- napari_tmidas/_tests/test_env_manager.py +89 -0
- napari_tmidas/_tests/test_grid_view_overlay.py +193 -0
- napari_tmidas/_tests/test_init.py +98 -0
- napari_tmidas/_tests/test_intensity_label_filter.py +222 -0
- napari_tmidas/_tests/test_label_inspection.py +86 -0
- napari_tmidas/_tests/test_processing_basic.py +500 -0
- napari_tmidas/_tests/test_processing_worker.py +142 -0
- napari_tmidas/_tests/test_regionprops_analysis.py +547 -0
- napari_tmidas/_tests/test_registry.py +70 -2
- napari_tmidas/_tests/test_scipy_filters.py +168 -0
- napari_tmidas/_tests/test_skimage_filters.py +259 -0
- napari_tmidas/_tests/test_split_channels.py +217 -0
- napari_tmidas/_tests/test_spotiflow.py +87 -0
- napari_tmidas/_tests/test_tyx_display_fix.py +142 -0
- napari_tmidas/_tests/test_ui_utils.py +68 -0
- napari_tmidas/_tests/test_widget.py +30 -0
- napari_tmidas/_tests/test_windows_basic.py +66 -0
- napari_tmidas/_ui_utils.py +57 -0
- napari_tmidas/_version.py +16 -3
- napari_tmidas/_widget.py +41 -4
- napari_tmidas/processing_functions/basic.py +557 -20
- napari_tmidas/processing_functions/careamics_env_manager.py +72 -99
- napari_tmidas/processing_functions/cellpose_env_manager.py +415 -112
- napari_tmidas/processing_functions/cellpose_segmentation.py +132 -191
- napari_tmidas/processing_functions/colocalization.py +513 -56
- napari_tmidas/processing_functions/grid_view_overlay.py +703 -0
- napari_tmidas/processing_functions/intensity_label_filter.py +422 -0
- napari_tmidas/processing_functions/regionprops_analysis.py +1280 -0
- napari_tmidas/processing_functions/sam2_env_manager.py +53 -69
- napari_tmidas/processing_functions/sam2_mp4.py +274 -195
- napari_tmidas/processing_functions/scipy_filters.py +403 -8
- napari_tmidas/processing_functions/skimage_filters.py +424 -212
- napari_tmidas/processing_functions/spotiflow_detection.py +949 -0
- napari_tmidas/processing_functions/spotiflow_env_manager.py +591 -0
- napari_tmidas/processing_functions/timepoint_merger.py +334 -86
- {napari_tmidas-0.2.2.dist-info → napari_tmidas-0.2.5.dist-info}/METADATA +71 -30
- napari_tmidas-0.2.5.dist-info/RECORD +63 -0
- napari_tmidas/_tests/__init__.py +0 -0
- napari_tmidas-0.2.2.dist-info/RECORD +0 -40
- {napari_tmidas-0.2.2.dist-info → napari_tmidas-0.2.5.dist-info}/WHEEL +0 -0
- {napari_tmidas-0.2.2.dist-info → napari_tmidas-0.2.5.dist-info}/entry_points.txt +0 -0
- {napari_tmidas-0.2.2.dist-info → napari_tmidas-0.2.5.dist-info}/licenses/LICENSE +0 -0
- {napari_tmidas-0.2.2.dist-info → napari_tmidas-0.2.5.dist-info}/top_level.txt +0 -0
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Metadata-Version: 2.4
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Name: napari-tmidas
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Version: 0.2.
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Version: 0.2.5
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Summary: A plugin for batch processing of confocal and whole-slide microscopy images of biological tissues
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Author: Marco Meer
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Author-email: marco.meer@pm.me
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Classifier: Framework :: napari
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Classifier: Intended Audience :: Developers
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Classifier: License :: OSI Approved :: BSD License
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Classifier: Operating System ::
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Classifier: Operating System :: MacOS
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Classifier: Operating System :: POSIX :: Linux
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Classifier: Programming Language :: Python
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Classifier: Programming Language :: Python :: 3
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Classifier: Programming Language :: Python :: 3.11
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Classifier: Topic :: Scientific/Engineering :: Image Processing
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License-File: LICENSE
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Requires-Dist: napari-ome-zarr
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Provides-Extra: clustering
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Provides-Extra: deep-learning
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Requires-Dist: napari-tmidas[clustering,deep-learning,testing]; extra == "all"
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# napari-tmidas
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[](https://github.com/macromeer/napari-tmidas/raw/main/LICENSE)
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[](https://pypi.org/project/napari-tmidas)
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[](https://python.org)
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[](https://pepy.tech/project/napari-tmidas)
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[](https://zenodo.org/badge/latestdoi/698257324)
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[](https://github.com/macromeer/napari-tmidas/actions)
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The `napari-tmidas` plugin consists of a growing collection of pipelines for fast batch processing of confocal and whole slide microscopy images of biological tissues. This is a WIP and based on the CLI version of [T-MIDAS](https://github.com/MercaderLabAnatomy/T-MIDAS).
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This napari plugin consists of a growing collection of pipelines for fast batch processing of confocal and whole slide microscopy images of biological tissues. This is a WIP and based on the [T-MIDAS terminal](https://github.com/MercaderLabAnatomy/T-MIDAS).
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## Features
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Currently, napari-tmidas provides pipelines as widgets for batch image conversion
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Currently, **napari-tmidas** provides pipelines as widgets for batch image conversion and processing, object cropping, label image inspection and ROI colocalization (cf. [usage](#usage) below). You can request new batch image processing features in [issues](https://github.com/MercaderLabAnatomy/napari-tmidas/issues).
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## Installation
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pip install napari-tmidas
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**For deep learning features** (Batch Crop Anything with SAM2, Spotiflow, Careamics, Trackastra), also install:
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pip install 'napari-tmidas[deep-learning]'
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Or install everything at once:
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pip install 'napari-tmidas[all]'
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It is recommended though to install the **latest development version**. Please also execute this command from time to time in the activated environment to benefit from newly added features:
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pip install git+https://github.com/
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pip install git+https://github.com/MercaderLabAnatomy/napari-tmidas.git
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### Additional Setup for Batch Crop Anything
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To use the Batch Crop Anything pipeline with SAM2, you need to install SAM2 separately:
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cd /opt # if the folder does not exist: mkdir /opt && cd /opt
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git clone https://github.com/facebookresearch/sam2.git && cd sam2
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~~sudo apt-get install zstd~~ # Pre-installed on Linux :man_shrugging:
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brew install zstd # for macOS (requires Homebrew)
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And you are done!
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And you are done!
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## Usage
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### Microscopy Image Conversion
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Converts `.lif, .nd2, .czi, .ndpi` and Acquifer data to TIF or OME-Zarr formats. Scan a folder, select files, and export with preserved spatial metadata.
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**Supported Formats:**
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- **TIF** - Standard format for compatibility
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- **OME-Zarr** - Recommended for large datasets, [spec v0.5](https://ngff.openmicroscopy.org/latest/) compliant with automatic physical metadata extraction (voxel sizes, spacing)
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<img src="https://github.com/user-attachments/assets/e377ca71-2f30-447d-825e-d2feebf7061b" alt="Microscopy Image Conversion Widget" style="width:75%; height:auto;">
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### Image Processing
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1. You start with entering the path to the folder containing the images to be processed (currently supports TIF, later also ZARR) and optionally a filter for filename suffix
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2. After indexing the files, a table appears with the found images. You can click on them to inspect them in the viewer.
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The image processing capabilities are powered by several excellent open-source tools:
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- [Cellpose 4](https://github.com/MouseLand/cellpose): Advanced cell segmentation
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- [Trackastra](https://github.com/weigertlab/trackastra): Cell tracking and analysis
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- [VisCy](https://github.com/mehta-lab/VisCy): Virtual staining using deep learning
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- [CAREamics](https://github.com/CAREamics/careamics): Content-aware image restoration and enhancement
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- [Spotiflow](https://github.com/weigertlab/spotiflow): Accurate and efficient spot detection for fluorescence microscopy
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#### Processing Function Documentation
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Detailed documentation for specific processing functions:
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**Core Processing**
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- [Basic Processing Functions](docs/basic_processing.md) - Label and intensity operations, channel splitting/merging, time series
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- [Cellpose Segmentation](docs/cellpose_segmentation.md) - Deep learning cell/nucleus segmentation
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- [TrackAstra Tracking](docs/trackastra_tracking.md) - Cell tracking across time-lapse data
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- [VisCy Virtual Staining](docs/viscy_virtual_staining.md) - Virtual staining of phase/DIC images using deep learning
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**Analysis and Quality Control**
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- [Grid View: Intensity + Labels Overlay](docs/grid_view_overlay.md) - Visual QC for segmentation results
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- [Intensity-Based Label Filtering](docs/intensity_label_filter.md) - Filter labels by signal intensity
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- [Regionprops Analysis](docs/regionprops_analysis.md) - Extract quantitative properties from labels
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**Advanced Processing**
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- [Advanced Processing Functions](docs/advanced_processing.md) - Denoising (CAREamics), spot detection (Spotiflow), SciPy/scikit-image filters, compression, colocalization
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### Batch Label Inspection
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If you have already segmented a folder full of images and now you want to maybe inspect and edit each label image, you can use the `Plugins > T-MIDAS > Batch Label Inspection`, which automatically saves your changes to the existing label image once you click the `Save Changes and Continue` button (bottom right).
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<img src="https://github.com/user-attachments/assets/0bf8c6ae-4212-449d-8183-e91b23ba740e" alt="Batch Label Inspection Widget" style="width:75%; height:auto;">
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### Crop Anything
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This pipeline combines the Segment Anything Model (SAM2; supports YX, ZYX and TYX data) for automatic object detection with an interactive interface for selecting and cropping multiple objects from images. To launch the widget, open `Plugins > T-MIDAS > Batch Crop Anything`. Cropping works like this: Enter 2D view and go to the first z slice where the object to be cropped is appearing. Activate/select the points layer and click on the object. Terminal shows progress. You can then proceed to select another object (always do this in 2D mode)
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<img src="https://github.com/user-attachments/assets/6d72c2a2-1064-4a27-b398-a9b86fcbc443" alt="Crop Anything Widget" style="width:75%; height:auto;">
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This pipeline quantifies colocalization between labeled regions of interest (ROIs) across multiple image channels. It determines the extent of overlap between ROIs in a reference channel and those in one or two other channels. The output is a table of colocalization counts. Optionally, the size of reference channel ROIs, as well as the total or median size of colocalizing ROIs in the other channels, can be included. Colocalization is determined using Boolean masking. The number of colocalizing instances is determined by counting unique label IDs within the overlapping regions. Typically, the reference channel contains larger structures, while other channels contain smaller, potentially nested, structures. For example, the reference channel might contain cell bodies, with the second and third channels containing nuclei and sub-nuclear objects, respectively.
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<img src="https://github.com/user-attachments/assets/2f9022a0-7b88-4588-a448-250f07a634d7" alt="ROI Colocalization Widget" style="width:75%; height:auto;">
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napari_tmidas/_crop_anything.py,sha256=aNrwZiIu9RIyo1wq_R0V24nCEgCFLFPLj0rvwqB6TZw,140754
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napari_tmidas/_processing_worker.py,sha256=vkaxjte4YJxlSCTCAtKJe85EeftVaBW7ym9OUQVZ_xU,10654
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