microlive 1.0.19__py3-none-any.whl → 1.0.20__py3-none-any.whl

microlive/__init__.py

@@ -23,7 +23,7 @@ Authors:
     Nathan L. Nowling, Brian Munsky, Ning Zhao
 """
 
-__version__ = "1.0.19"
+__version__ = "1.0.20"
 __author__ = "Luis U. Aguilera, William S. Raymond, Rhiannon M. Sears, Nathan L. Nowling, Brian Munsky, Ning Zhao"
 
 # Package name (for backward compatibility)

microlive/gui/app.py

@@ -7859,9 +7859,12 @@ class GUI(QMainWindow):
                 z_spot_size_in_px=z_spot_size,
                 use_3d=use_3d
             )
-            threshold = auto_thresh.calculate()
+            threshold_raw = auto_thresh.calculate()
             method_used = auto_thresh.method_used
             
+            # Reduce threshold by 10% to improve spot coverage (auto-threshold tends to overestimate)
+            threshold = threshold_raw * 0.9
+            
             # Store per-channel
             self.auto_threshold_per_channel[channel] = threshold
             

{microlive-1.0.19.dist-info → microlive-1.0.20.dist-info}/METADATA

@@ -1,6 +1,6 @@
 Metadata-Version: 2.4
 Name: microlive
-Version: 1.0.19
+Version: 1.0.20
 Summary: Live-cell microscopy image analysis and single-molecule measurements
 Project-URL: Homepage, https://github.com/ningzhaoAnschutz/microlive
 Project-URL: Documentation, https://github.com/ningzhaoAnschutz/microlive/blob/main/docs/user_guide.md

{microlive-1.0.19.dist-info → microlive-1.0.20.dist-info}/RECORD

@@ -1,4 +1,4 @@
-microlive/__init__.py,sha256=x5itFGRF8M9JE08BfNbfCkp9JerbxKsTXr7mskW6CUE,1385
+microlive/__init__.py,sha256=doE9QrxZz6Z6QDuG_-XE5waL5t5mZX0E1bu0yx4Cs1E,1385
 microlive/imports.py,sha256=wMJNmtG06joCJNPryktCwEKz1HCJhfGcm3et3boINuc,7676
 microlive/microscopy.py,sha256=OFqf0JXJW4-2cLHvXnwwp_SfMFsUXwp5lDKbkCRR4ok,710841
 microlive/ml_spot_detection.py,sha256=pVbOSGNJ0WWMuPRML42rFwvjKVZ0B1fJux1179OIbAg,10603
@@ -7,7 +7,7 @@ microlive/data/icons/__init__.py,sha256=47DEQpj8HBSa-_TImW-5JCeuQeRkm5NMpJWZG3hS
 microlive/data/icons/icon_micro.png,sha256=b5tFv4E6vUmLwYmYeM4PJuxLV_XqEzN14ueolekTFW0,370236
 microlive/data/models/__init__.py,sha256=47DEQpj8HBSa-_TImW-5JCeuQeRkm5NMpJWZG3hSuFU,0
 microlive/gui/__init__.py,sha256=tB-CdDC7x5OwYFAQxLOUvfVnUThaXKXVRsB68YP0Y6Q,28
-microlive/gui/app.py,sha256=tQTFbTkbT_NRMFVdw8fnsA5krrUczZANEscQOcuZRGQ,848759
+microlive/gui/app.py,sha256=hQbAiLeb_3FyXXptqNVvo_4V_oh-Z0C2wnvdXHF7wPc,848922
 microlive/gui/main.py,sha256=b66W_2V-pclGKOozfs75pwrCGbL_jkVU3kFt8RFMZIc,2520
 microlive/gui/micro_mac.command,sha256=TkxYOO_5A2AiNJMz3_--1geBYfl77THpOLFZnV4J2ac,444
 microlive/gui/micro_windows.bat,sha256=DJUKPhDbCO4HToLwSMT-QTYRe9Kr1wn5A2Ijy2klIrw,773
@@ -20,8 +20,8 @@ microlive/utils/device.py,sha256=tcPMU8UiXL-DuGwhudUgrbjW1lgIK_EUKIOeOn0U6q4,253
 microlive/utils/model_downloader.py,sha256=EruviTEh75YBekpznn1RZ1Nj8lnDmeC4TKEnFLOow6Y,9448
 microlive/utils/resources.py,sha256=Jz7kPI75xMLCBJMyX7Y_3ixKi_UgydfQkF0BlFtLCKs,1753
 microlive/data/models/spot_detection_cnn.pth,sha256=Np7vpPJIbKQmuKY0Hx-4IkeEDsnks_QEgs7TqaYgZmI,8468580
-microlive-1.0.19.dist-info/METADATA,sha256=XJvzWLd4-xz5XX-D67zxOWPrE_-XM76g5GScvzUyubw,12462
-microlive-1.0.19.dist-info/WHEEL,sha256=WLgqFyCfm_KASv4WHyYy0P3pM_m7J5L9k2skdKLirC8,87
-microlive-1.0.19.dist-info/entry_points.txt,sha256=Zqp2vixyD8lngcfEmOi8fkCj7vPhesz5xlGBI-EubRw,54
-microlive-1.0.19.dist-info/licenses/LICENSE,sha256=ixuiBLtpoK3iv89l7ylKkg9rs2GzF9ukPH7ynZYzK5s,35148
-microlive-1.0.19.dist-info/RECORD,,
+microlive-1.0.20.dist-info/METADATA,sha256=60aFE4XiBUslLfHS3k-BxN4XhHe4UR7BTl6-4E7Z5Ec,12462
+microlive-1.0.20.dist-info/WHEEL,sha256=WLgqFyCfm_KASv4WHyYy0P3pM_m7J5L9k2skdKLirC8,87
+microlive-1.0.20.dist-info/entry_points.txt,sha256=Zqp2vixyD8lngcfEmOi8fkCj7vPhesz5xlGBI-EubRw,54
+microlive-1.0.20.dist-info/licenses/LICENSE,sha256=ixuiBLtpoK3iv89l7ylKkg9rs2GzF9ukPH7ynZYzK5s,35148
+microlive-1.0.20.dist-info/RECORD,,