geney 1.3.78__py2.py3-none-any.whl → 1.4.0__py2.py3-none-any.whl

geney/_survival_utils.py

@@ -0,0 +1,143 @@
+import pandas as pd
+import numpy as np
+import matplotlib.pyplot as plt
+from pathlib import Path
+# from scipy.integrate import trapezoid
+from geney.utils import unload_pickle, unload_json, contains
+from lifelines.exceptions import ConvergenceError
+from lifelines import KaplanMeierFitter
+from lifelines.statistics import logrank_test
+from lifelines import CoxPHFitter
+
+pd.set_option('display.max_columns', None)
+pd.options.mode.chained_assignment = None
+
+
+def prepare_clinical_data(df=None):
+    if df is None:
+        CLINICAL_DATA_FILE = Path('/tamir2/yoramzar/Projects/Cancer_mut/Explore_data/reports/df_p_all.pkl')
+        df = unload_pickle(CLINICAL_DATA_FILE)
+
+    df.rename(columns={'patient_uuid': 'case_id'}, inplace=True)
+    cols = list(df.columns)
+    cols_days_to_followup = [col for col in cols if 'days_to_followup' in col] + [col for col in cols if 'days_to_last_followup' in col]
+    cols_days_to_know_alive = [col for col in cols if 'days_to_know_alive' in col] + [col for col in cols if 'days_to_last_known_alive' in col]
+    cols_days_to_death = [col for col in cols if 'days_to_death' in col]
+    cols_duration = cols_days_to_followup + cols_days_to_know_alive + cols_days_to_death
+    col_vital_status = 'days_to_death'
+    event_col_label = 'event'
+    duration_col_label = 'duration'
+    df.insert(1, event_col_label, df.apply(lambda x: int(not np.isnan(x[col_vital_status])), axis=1))
+    df.insert(1, duration_col_label, df.apply(lambda x: max([x[col] for col in cols_duration if not np.isnan(x[col])], default=-1), axis=1))
+    df[duration_col_label] /= 365
+    df = df.query(f"{duration_col_label}>=0.0")[['duration', 'event', 'case_id', 'chemotherapy', 'hormone_therapy', 'immunotherapy', 'targeted_molecular_therapy', 'Proj_name']]
+    # df.to_csv('/tamir2/nicolaslynn/data/tcga_metadata/tcga_clinical_data.csv')
+    return df
+
+
+class SurvivalAnalysis:
+    def __init__(self, clindf=None):
+        self.clindf = prepare_clinical_data(clindf)
+        self.treatment_features = ['chemotherapy', 'hormone_therapy', 'immunotherapy', 'targeted_molecular_therapy']
+        self.df = self.clindf.copy()
+        self.df['group'] = 0
+        self.df.fillna(0, inplace=True)
+        self.treatment_features = ['chemotherapy', 'hormone_therapy', 'immunotherapy', 'targeted_molecular_therapy']
+
+    def generate_clinical_dataframe(self, target_cases, control_cases=None, inplace=False, features_of_interest=[]):
+        df = self.df.copy()
+        df.loc[df[df.case_id.isin(target_cases)].index, 'group'] = 2
+        if control_cases is not None:
+            df.loc[df[df.case_id.isin(control_cases)].index, 'group'] = 1
+
+        df = df[df.group > 0]
+        df.group -= 1
+        core_features = ['duration', 'event']
+        df = df[core_features + features_of_interest]
+
+        for col in self.treatment_features:
+            if col not in df:
+                continue
+            df.loc[df[col] > 0, col] = 1
+
+        df = df[core_features + [col for col in features_of_interest if
+                                 df[col].nunique() > 1]]  # and df[col].value_counts(normalize=True).min() >= 0.01]]
+        return df
+
+    def kaplan_meier_analysis(self, df, control_label='Unaffected Patients', target_label='Affected Patients', feature='group', plot=False, title=None, time_cap=False, savepath=None, figsize=(7, 3), tmb_p_value=None):
+        # Can only be performed on features with two unique values
+        cap_time = df.groupby(feature).duration.max().min()
+        # df['duration'] = df['duration'].clip(upper=cap_time)
+        auc_vals = []
+        results = pd.Series()
+        count = 0
+        for val in [0, 1]:
+            g = df[df[feature] == val]
+            kmf = KaplanMeierFitter()
+            label = f"{control_label} ({len(g)} cases)" if val == 0 else f"{target_label} ({len(g)} cases)"
+            if val == 0:
+                results[control_label] = len(g)
+            else:
+                results[target_label] = len(g)
+
+            kmf.fit(g['duration'], g['event'], label=label)
+            surv_func = kmf.survival_function_
+            filtered_surv_func = surv_func[surv_func.index <= cap_time]
+            auc = np.trapz(filtered_surv_func[label], filtered_surv_func.index)
+            # auc = trapz(surv_func[label], surv_func.index)
+            auc_vals.append(auc)
+            if plot:
+                if count == 0:
+                    fig, ax = plt.subplots(figsize=figsize)
+                    kmf.plot_survival_function(ax=ax, ci_show=True, color="#2430e0", lw=2)
+                else:
+                    kmf.plot_survival_function(ax=ax, ci_show=True, color="#e60215", lw=2)
+                count += 1
+
+        p_value = self.log_rank(df[df[feature] == 1], df[df[feature] == 0])
+
+        if plot:
+            ax.text(0.6, 0.6, rf'Survival $p{{v}}$: {p_value:.3e}', transform=ax.transAxes, fontsize=10,
+                    horizontalalignment='left')
+            if tmb_p_value:
+                ax.text(0.6, 0.53, rf'TMB $p{{v}}$: {tmb_p_value:.3e}', transform=ax.transAxes, fontsize=10,
+                        horizontalalignment='left')
+            # Grid and spines
+            ax.grid(True, which="major", linestyle="--", linewidth=0.5, color="grey", alpha=0.7)
+            ax.spines['top'].set_visible(False)
+            ax.spines['right'].set_visible(False)
+            ax.tick_params(axis="both", which="major", labelsize=10)
+            if title:
+                ax.set_title(title, fontsize=12)
+            legend = ax.legend(fontsize=9, loc='best', frameon=True)
+            legend.get_frame().set_facecolor('white')  # Set the background color to white
+            legend.get_frame().set_edgecolor('black')  # Set the edge color to black
+            plt.xlabel('Time (years)')
+            plt.ylabel('Survival Probability')
+            if time_cap:
+                plt.xlim([0, cap_time])
+            plt.tight_layout()
+            if savepath is not None:
+                plt.savefig(savepath, bbox_inches='tight', dpi=300)
+            plt.show()
+
+        results['p_value'] = p_value
+        results['auc_target'] = auc_vals[-1]
+        if len(auc_vals) > 1:
+            results['auc_delta'] = auc_vals[-1] - auc_vals[0]
+            results['auc_control'] = auc_vals[0]
+
+        return results
+
+    def log_rank(self, group1, group2):
+        return logrank_test(group1['duration'], group2['duration'],
+                            event_observed_A=group1['event'],
+                            event_observed_B=group2['event']).p_value
+
+    def perform_cox_analysis(self, df, features_of_interest):
+        # Very simple... will return a series with p values for each feature
+        try:
+            return CoxPHFitter().fit(df[features_of_interest + ['duration', 'event']], 'duration', 'event').summary.p
+        except ConvergenceError:
+            print("Convergence Error")
+            return pd.Series()

geney/_tcga_utils.py

@@ -0,0 +1,405 @@
+import pandas as pd
+import random
+from pathlib import Path
+from tqdm import tqdm
+
+class TCGACase:
+    def __init__(self, df):
+        # Here we get a dataframe of mutations within a gene
+        self.df = df
+        self.calculate_vaf()
+        self.space_variants(spacer_size=50)
+        self.case_id = df.case_id.tolist()[0]
+
+    def space_variants(self, spacer_size=100, group_likelihood_threshold=0):
+        df = self.df
+        if df.empty:
+            df['group'] = 0
+            return self
+        values = sorted(df.Start_Position.unique().tolist())
+        # groups = [list(group) for key, group in groupby(values, key=lambda x: (x - values[values.index(x) - 1] >
+        # spacer_size) if values.index(x) > 0 else False)] Initialize variables
+        groups = []
+        current_group = []
+
+        # Iterate through the values
+        for i in range(len(values)):
+            if i == 0:
+                current_group.append(values[i])
+            else:
+                if values[i] - values[i - 1] <= spacer_size:
+                    current_group.append(values[i])
+                else:
+                    groups.append(current_group)
+                    current_group = [values[i]]
+
+        # Append the last group if it's not empty
+        if current_group:
+            groups.append(current_group)
+
+        df.loc[:, 'group'] = 0
+        for i, g in enumerate(groups):
+            df.loc[df.Start_Position.isin(g), 'group'] = i
+        self.df = df
+        return self
+
+    def calculate_vaf(self):
+        df = self.df
+        df = df[df.t_depth > 0]
+        df.loc[:, 'vaf'] = df.apply(lambda row: row.t_alt_count / row.t_depth, axis=1)
+        self.df = df
+        return self
+
+    def find_overlayed_variants(self):
+        df = self.df
+        mut_counts = df.mut_id.value_counts()
+        mut_counts = mut_counts[mut_counts > 1].index
+
+        small_df = df.groupby('mut_id', as_index=False).agg({
+            't_depth': 'sum',
+            't_alt_count': 'sum',
+            't_ref_count': 'sum',
+        })
+
+        df = df.drop_duplicates(subset='mut_id', keep='first')
+
+        small_df = small_df[small_df.t_depth > 0]
+        small_df['vaf'] = small_df.t_alt_count / small_df.t_depth
+
+        small_df = small_df.set_index('mut_id')
+        df.set_index('mut_id', inplace=True)
+        df.update(small_df)
+        df.reset_index(inplace=True)
+        self.df = df
+        return self
+
+    def find_epistasis(self, pth=3, rth=0):
+        df = self.df
+        if df.empty:
+            return None
+        # df = df[df.t_alt_count > rth].sort_values('Start_Position', ascending=True)
+        df = df[(df.t_alt_count > df.t_ref_count / pth) & (df.t_alt_count >= rth)].sort_values('Start_Position',
+                                                                                               ascending=True)
+
+        # display(df[['mut_id', 't_alt_count', 't_ref_count']])
+
+        # Group by the group_key
+        grouped = df.groupby('group').agg({
+            'mut_id': lambda x: '|'.join(x),
+            't_alt_count': 'mean',
+            't_ref_count': 'mean',
+            'case_id': 'first'
+        }).reset_index(drop=True)
+
+        # Drop the group_key column
+        return grouped[grouped.mut_id.str.contains('\|')][['mut_id', 't_alt_count', 't_ref_count', 'case_id']]
+
+
+class TCGAGene:
+    def __init__(self, gene, cancer_path=Path('/tamir2/cancer_proj/gdc_db/data/filtered_feb_2021/AllGenes/'),
+                 valid_cases=None, extra_cols=[], exclude_filters=None, include_filter=None):
+        file_path = cancer_path / gene / 'GeneMutTble.txt'
+        if not file_path.exists():
+            self.df = pd.DataFrame()
+
+        else:
+            df = pd.read_csv(file_path,
+                             usecols=['Variant_Type', 'FILTER', 'vcf_tumor_gt', 'vcf_normal_gt',
+                                      'COSMIC', 't_depth', 't_ref_count', 't_alt_count', 'Proj_name',
+                                      'HGVSc', 'Chromosome', 'Start_Position', 'Reference_Allele',
+                                      'Tumor_Seq_Allele2', 'case_id', 'Gene_name', 'Variant_Type',
+                                      'Variant_Classification'] + extra_cols,
+                             low_memory=False).sort_values('Start_Position', ascending=True)
+
+            df['attention'] = True
+
+            if df.empty:
+                self.df = df
+
+            else:
+                df = df[df.Variant_Type.isin(['SNP', 'INS', 'DEL'])]
+                df = df.astype({'Start_Position': int})
+
+                if include_filter is not None:
+                    # df = df[df.FILTER == include_filter]
+                    df.loc[~df['FILTER'].str.contains(include_filter), 'attention'] = False
+
+                elif exclude_filters is not None:
+                    for exclude_filter in exclude_filters:
+                        # df = df[~df.FILTER.str.contains(exclude_filter)]
+                        df.loc[df['FILTER'].str.contains(exclude_filter), 'attention'] = False
+
+                if valid_cases is not None:
+                    # df = df[df.case_id.isin(valid_cases)]
+                    df.loc[~df.case_id.isin(valid_cases), 'attention'] = False
+
+                df['mut_id'] = df.apply(lambda
+                                            row: f"{row.Gene_name}:{row.Chromosome.replace('chr', '')}:{row.Start_Position}:{row.Reference_Allele}:{row.Tumor_Seq_Allele2}",
+                                        axis=1)
+                df['mut_id_yoram'] = df.apply(lambda
+                                                  row: f"{row.Gene_name}:{row.Chromosome}:{row.Variant_Classification}:{row.Start_Position}:{row.Reference_Allele}:{row.Tumor_Seq_Allele2}",
+                                              axis=1)
+                silent_mut_classes = ["3'Flank", "3'UTR", "Silent", "Splice_Site", "Splice_Region", "Intron", "5'Flank",
+                                      "3'Flank"]
+                df['silent'] = df.apply(lambda row: row.Variant_Classification in silent_mut_classes, axis=1)
+                df['ratio'] = df.t_alt_count + df.t_ref_count
+                df = df[df.ratio > 0]
+                df['ratio'] = df.t_alt_count / df.ratio
+                self.df = df
+
+    def __repr__(self):
+        return repr(self.df[self.df.attention])
+
+    @property
+    def data(self):
+        return self.df[self.df.attention]
+
+    def affected_cases(self, mut_id=None, read_ratio=0, filters=[]):
+        if mut_id is None:
+            return self.df.case_id.unique().tolist()
+        df = self.df
+        df = df[(df.mut_id == mut_id) & (df.ratio >= read_ratio)]
+        for filter in filters:
+            df = df[~df.FILTER.str.contains(filter)]
+        return df.case_id.unique().tolist()
+
+    def get_patient_muts(self, case_id=None, read_ratio=0, exclude_filters=None):
+        if case_id is None:
+            case_id = random.choice(self.affected_cases())
+        return self.df[self.df.case_id == case_id]
+
+    def get_patients_affected(self, mut_id, read_ratio=0, exclude_filters=None):
+        return self.data[self.data.mut_id == mut_id].case_id.unique().tolist()
+
+
+    def get_patients_unaffected(self, mut_id, must_contain_all=False, read_ratio=0, exclude_filters=None):
+        # returns all patients not affected by ALL the mutation in mut id (patients containg individual mutations only allowed) unless must_contain_all= True
+        pass
+
+    def split_patients(self, mut_id, strict=True):
+        # returns two lists: all patients affected by a mutation and all patients with none of the mutations (or the mutations but not togehter)
+        pass
+
+    def arrange_patients_by_project(self, mut_id):
+        # returns all the patients affected by a mutation grouped by cancer project
+        pass
+
+    def total_prevalence(self, mut_id):
+        pass
+
+    def project_prevalence(self, mut_id, df_p_proc):
+        mut_prevalence = {}
+        for i, g in tqdm(self.data.groupby(['mut_id', 'Transcript_ID'])):
+            mut_prevalence[i] = series_to_pretty_string((df_p_proc[g.case_id].value_counts() / project_counts).dropna())
+        return pd.Series(mut_prevalence)
+
+    def project_counts(self, mut_id):
+        pass
+
+    def filter_silent_muts(self):
+        self.df.loc[self.df.silent, 'attention'] = False
+        return self
+
+
+def series_to_pretty_string(series):
+    # Format each index-value pair, applying scientific notation to floats with 3 significant figures
+    pretty_str = "\n".join([
+        f"{index}: {value:.3e}" if isinstance(value, float) else f"{index}: {value}"
+        for index, value in series.items()
+    ])
+    return pretty_str
+
+
+# CLINICAL_DATA_FILE = Path('/tamir2/nicolaslynn/data/TCGA/cancer_reports/new_df_p_proc.pkl')
+# CLINICAL_DATA_FILE = Path('/tamir2/yoramzar/Projects/Cancer_mut/Explore_data/reports/df_p_all.pkl')
+# CANCER_DATA_PATH = Path('/tamir2/cancer_proj/gdc_db/data/filtered_feb_2021/AllGenes')
+# MAF_FILE_NAME = 'GeneMutTble.txt'
+# CASE_TRACKER = pd.read_csv('/tamir2/nicolaslynn/projects/TCGAParsed/case2proj.csv', index_col=0)
+# PROJ_COUNTS = CASE_TRACKER.proj.value_counts()
+# OKGP_DATA_FILE = Path('/tamir2/nicolaslynn/projects/1000GenomesProjMutations/parsed_1kgp_mutations_in_target_genes.csv')
+# MUTATION_FREQ_DF = pd.read_csv(OKGP_DATA_FILE, index_col=0)
+# PROTEIN_ANNOTATIONS = pd.read_csv('/tamir2/nicolaslynn/data/BioMart/protein_annotations.csv').rename(columns={'Interpro start': 'start', 'Interpro end': 'end', 'Interpro Short Description': 'name'})[['Gene stable ID', 'Transcript stable ID', 'start', 'end', 'name']]
+# PROTEIN_ANNOTATIONS['length'] = PROTEIN_ANNOTATIONS.apply(lambda row: abs(row.start - row.end), axis=1)
+
+# def prepare_gene_sets():
+#     # gene_annotations_file = Path('/tamir2/nicolaslynn/data/COSMIC/cancer_gene_roles.csv')
+#     # GENE_DF = pd.read_csv(gene_annotations_file, index_col=0)
+#     # all_oncogenes = GENE_DF[GENE_DF.OG==True].index.tolist()
+#     # all_oncogenes = list(set(all_oncogenes))
+#     return [], [], []
+#
+# CLIN_DF = prepare_clinical_data()
+# TSGS, ONCOGENES, CANCER_GENES = prepare_gene_sets()
+#
+#
+# def generate_survival_quantitative(affected_df, nonaffected_df):
+#     if affected_df.empty or nonaffected_df.empty:
+#         return np.nan, np.nan, np.nan
+#     results = logrank_test(affected_df['duration'], nonaffected_df['duration'],
+#                            event_observed_A=affected_df['event'],
+#                            event_observed_B=nonaffected_df['event'])
+#     p_value = results.p_value
+#     kmf = KaplanMeierFitter()
+#     kmf.fit(affected_df['duration'], affected_df['event'], label=f'With Epistasis ({len(affected_df)})')
+#     times, surv_probs = kmf.survival_function_.index.values, kmf.survival_function_.values.flatten()
+#     auc1 = np.trapz(surv_probs, times)
+#     kmf.fit(nonaffected_df['duration'], nonaffected_df['event'], label=f'Without Epistasis ({len(nonaffected_df)})')
+#     times, surv_probs = kmf.survival_function_.index.values, kmf.survival_function_.values.flatten()
+#     auc2 = np.trapz(surv_probs, times)
+#     return p_value, auc1, auc2
+#
+# def generate_survival_pvalue(affected_df, unaffected_df):
+#     results = logrank_test(affected_df['duration'], unaffected_df['duration'],
+#                            event_observed_A=affected_df['event'],
+#                            event_observed_B=unaffected_df['event'])
+#
+#     p_value = results.p_value
+#     kmf = KaplanMeierFitter()
+#     # Fit data
+#     kmf.fit(affected_df['duration'], affected_df['event'], label=f'Without Epistasis ({len(affected_df)})')
+#     ax = kmf.plot()
+#
+#     kmf.fit(unaffected_df['duration'], unaffected_df['event'], label=f'With Epistasis ({len(unaffected_df)})')
+#     kmf.plot(ax=ax)
+#     plt.text(5, 0.95, f'pval: {p_value:.3e}')
+#     plt.show()
+#     return p_value
+#
+# def get_project_prevalence(cases_affected):
+#     ca = [c for c in cases_affected if c in CASE_TRACKER.index]
+#     prevalences = CASE_TRACKER.loc[ca].proj.value_counts() / PROJ_COUNTS
+#     prevalences.fillna(0, inplace=True)
+#     prevalences = prevalences[[i for i in prevalences.index if 'TCGA' in i]]
+#     prevalences.index = [s.replace('TCGA', 'prev') for s in prevalences.index]
+#     return prevalences
+#
+# def get_project_counts(cases_affected):
+#     ca = [c for c in cases_affected if c in CASE_TRACKER.index]
+#     prevalences = CASE_TRACKER.loc[ca].proj.value_counts()
+#     prevalences = prevalences[[i for i in prevalences.index if 'TCGA' in i]]
+#     prevalences.index = [s.replace('TCGA_', '') for s in prevalences.index]
+#     return prevalences
+#
+# def get_event_consequence(df):
+#     assert df.Transcript_ID.nunique() == 1, 'Too many transcripts to return a single consequenc.'
+#     return df.iloc[0].Consequence
+#
+# def get_dbSNP_id(df):
+#     return df.iloc[0].dbSNP_RS
+#
+# def load_variant_file(gene):
+#     df = pd.read_csv(CANCER_DATA_PATH / gene / MAF_FILE_NAME, low_memory=False)
+#     df['mut_id'] = df.apply(lambda row: f"{row.Gene_name}:{row.Chromosome.replace('chr', '')}:{row.Start_Position}:{row.Reference_Allele}:{row.Tumor_Seq_Allele2}", axis=1)
+#     return df
+#
+# def find_event_data(event):
+#     df = load_variant_file(event.gene)
+#     if df.empty:
+#         return None
+#
+#     df = df.query \
+#         ('Chromosome == @event.chromosome & Start_Position == @event.start & Reference_Allele == @event.ref & Tumor_Seq_Allele2 == @event.alt')
+#
+#     if df.empty:
+#         return None
+#
+#     if event.transcript_id is not None:
+#         df = df[df.Transcript_ID == event.transcript_id]
+#     df['mut_id'] = event.event_id
+#     return df
+#
+#
+# class GEvent:
+#     def __init__(self, event_id, transcript_id=None):
+#         self.gene, self.chromosome, self.start, self.ref, self.alt = event_id.split(':')
+#         self.transcript_id = transcript_id
+#         self.chromosome = f'chr{self.chromosome}'
+#         self.start = int(self.start)
+#         self.event_id = event_id
+#
+#
+#
+# def get_okgp_mutation_frequency(mut_id):
+#     if mut_id in MUTATION_FREQ_DF.index:
+#         return MUTATION_FREQ_DF.loc[mut_id].cases_affected
+#     else:
+#         return 0
+#
+# def get_df_filter_info(df):
+#     filter_artifact_values: list = ["oxog", "bPcr", "bSeq"]
+#     MuTect2_filters: list = ['Germline risk', 't_lod_fstar', 'alt_allele_in_normal', 'panel_of_normals', 'clustered_events',
+#                              'str_contraction', 'multi_event_alt_allele_in_normal', 'homologous_mapping_event', 'triallelic_site']
+#     filter_col_name: str = "FILTER_info"  # column name to add to the dataframe
+#     filter_info_list: list = []
+#     f_cnr_info = {}
+#
+#     for j, (prj, df_prj) in enumerate(df.groupby('Proj_name')):
+#         filter_vals = list(df_prj['FILTER'])
+#         num_pass, num_artifacts, num_mutect2_filters = 0, 0, 0
+#         for filter_val in filter_vals:
+#             num_pass += ('PASS' in filter_val)
+#             num_artifacts += any([x in filter_val for x in filter_artifact_values])
+#             num_mutect2_filters += any([x in filter_val for x in MuTect2_filters])
+#         num_rest = max(0, (len(filter_vals) - num_pass - num_artifacts - num_mutect2_filters))
+#         f_cnr_info[str(prj)[5:]] = (num_pass, num_mutect2_filters, num_artifacts, num_rest)
+#     return f_cnr_info
+#
+# def yoram_mutid(row):
+#     return f'{row.Gene_name}:{row.Chromosome}:{row.Consequence}:{row.Start_Position}:{row.Reference_Allele}:{row.Tumor_Seq_Allele2}'
+#
+#
+# def annotate_level_two(mut_id, tid):
+#     mut = GEvent(mut_id, tid)
+#     df = find_event_data(mut)
+#
+#     if df.empty or df is None:
+#         return None
+#
+#     patients_affected = df.cases_affected.unique().tolist()
+#     p_val, auc_a, auc_n = generate_survival_quantitative(CLIN_DF[CLIN_DF.case_id.isin(patients_affected)], CLIN_DF[~CLIN_DF.case_id.isin(patients_affected)])
+#     project_prevalences = get_project_prevalence(patients_affected)
+#     prev_dict = project_prevalences.to_dict().sort()
+#     project_counts = get_project_counts(patients_affected)
+#
+#     s = pd.Series({
+#         'mut_id': mut_id,
+#         'yoram_mut_id': yoram_mutid(df.iloc[0]),
+#         'transcript_id': tid,
+#         'affected_cases': len(patients_affected),
+#         'dbSNP_id': get_dbSNP_id(df),
+#         'consequence': get_event_consequence(df),
+#         'survival_p_value': p_val,
+#         'auc_affected': auc_a,
+#         'auc_nonaffected': auc_n,
+#         'TSG': contains(TSGS, mut.gene),
+#         'oncogene': contains(ONCOGENES, mut.gene),
+#         'cases_1kgp': get_okgp_mutation_frequency(mut.event_id),
+#         'filter_inf': get_df_filter_info(df),
+#         'strand': df.Strand.unique().tolist()[0],
+#         'prevalences': prev_dict
+#     })
+#
+#     s['max_prev'] = project_prevalences.max()
+#     s['rel_proj'] = ','.join([c.split('_')[-1] for c in project_prevalences[project_prevalences == project_prevalences.max()].index.tolist()])
+#     s = pd.concat([s, project_prevalences, project_counts])
+#     del df
+#     return s
+#
+# def get_mut_counts():
+#     cases = unload_json('/tamir2/nicolaslynn/projects/TCGAParsed/recurring_single_muts_tcga.json')
+#     cases = pd.Series(cases)
+#     cases.name = 'num_cases'
+#     cases.index.name = 'mut_id'
+#     cases = cases.to_frame()
+#     cases.reset_index(inplace=True)
+#     return cases
+#
+#
+def create_mut_id(row):
+    return f"{row.Gene_name}:{row['Chromosome']}:{row['Start_Position']}:{row['Reference_Allele']}:{row['Tumor_Seq_Allele2']}"
+#
+#
+# def is_in_exon(mut_id, tid):
+#     from geney.Gene import Gene
+#     transcript = Gene(mut_id.split(':')[0]).generate_transcript(tid)
+#     return int(mut_id.split(':')[2]) in transcript.exonic_indices