canns 0.14.3__py3-none-any.whl → 0.15.0__py3-none-any.whl

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Files changed (28) hide show
  1. canns/analyzer/data/asa/__init__.py +77 -21
  2. canns/analyzer/data/asa/coho.py +97 -0
  3. canns/analyzer/data/asa/cohomap.py +408 -0
  4. canns/analyzer/data/asa/cohomap_scatter.py +10 -0
  5. canns/analyzer/data/asa/cohomap_vectors.py +311 -0
  6. canns/analyzer/data/asa/cohospace.py +173 -1153
  7. canns/analyzer/data/asa/cohospace_phase_centers.py +137 -0
  8. canns/analyzer/data/asa/cohospace_scatter.py +1220 -0
  9. canns/analyzer/data/asa/embedding.py +3 -4
  10. canns/analyzer/data/asa/plotting.py +4 -4
  11. canns/analyzer/data/cell_classification/__init__.py +10 -0
  12. canns/analyzer/data/cell_classification/core/__init__.py +4 -0
  13. canns/analyzer/data/cell_classification/core/btn.py +272 -0
  14. canns/analyzer/data/cell_classification/visualization/__init__.py +3 -0
  15. canns/analyzer/data/cell_classification/visualization/btn_plots.py +258 -0
  16. canns/analyzer/visualization/__init__.py +2 -0
  17. canns/analyzer/visualization/core/config.py +20 -0
  18. canns/analyzer/visualization/theta_sweep_plots.py +142 -0
  19. canns/pipeline/asa/runner.py +19 -19
  20. canns/pipeline/asa_gui/__init__.py +5 -3
  21. canns/pipeline/asa_gui/analysis_modes/pathcompare_mode.py +3 -1
  22. canns/pipeline/asa_gui/core/runner.py +23 -23
  23. canns/pipeline/asa_gui/views/pages/preprocess_page.py +7 -12
  24. {canns-0.14.3.dist-info → canns-0.15.0.dist-info}/METADATA +1 -1
  25. {canns-0.14.3.dist-info → canns-0.15.0.dist-info}/RECORD +28 -20
  26. {canns-0.14.3.dist-info → canns-0.15.0.dist-info}/WHEEL +0 -0
  27. {canns-0.14.3.dist-info → canns-0.15.0.dist-info}/entry_points.txt +0 -0
  28. {canns-0.14.3.dist-info → canns-0.15.0.dist-info}/licenses/LICENSE +0 -0
@@ -0,0 +1,311 @@
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+ """Stripe vectors and diagnostics for CohoMap."""
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+
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+ from __future__ import annotations
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+
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+ import os
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+ from typing import Any
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+
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+ import matplotlib.pyplot as plt
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+ import numpy as np
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+ from scipy.ndimage import rotate
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+
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+ from ...visualization.core import PlotConfig, finalize_figure
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+ from .cohomap import fit_cohomap_stripes
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+
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+
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+ def _ensure_plot_config(
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+ config: PlotConfig | None,
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+ factory,
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+ *args,
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+ **defaults,
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+ ) -> PlotConfig:
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+ if config is None:
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+ return factory(*args, **defaults)
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+ return config
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+
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+
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+ def _ensure_parent_dir(save_path: str | None) -> None:
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+ if save_path:
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+ parent = os.path.dirname(save_path)
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+ if parent:
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+ os.makedirs(parent, exist_ok=True)
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+
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+
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+ def _rot_para(params1: np.ndarray, params2: np.ndarray) -> tuple[np.ndarray, np.ndarray]:
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+ if abs(np.cos(params1[0])) < abs(np.cos(params2[0])):
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+ y = params1.copy()
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+ x = params2.copy()
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+ else:
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+ x = params1.copy()
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+ y = params2.copy()
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+
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+ alpha = y[0] - x[0]
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+ if (alpha < 0) and (abs(alpha) > np.pi / 2):
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+ x[0] += np.pi
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+ x[0] = x[0] % (2 * np.pi)
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+ elif (alpha < 0) and (abs(alpha) < np.pi / 2):
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+ x[0] += np.pi * 4 / 3
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+ x[0] = x[0] % (2 * np.pi)
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+ elif abs(alpha) > np.pi / 2:
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+ y[0] -= np.pi / 3
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+ y[0] = y[0] % (2 * np.pi)
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+
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+ if y[0] > np.pi / 2:
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+ y[0] -= np.pi / 6
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+ x[0] -= np.pi / 6
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+ x[0] = x[0] % (2 * np.pi)
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+ y[0] = y[0] % (2 * np.pi)
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+ if x[0] > np.pi / 2:
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+ y[0] += np.pi / 6
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+ x[0] += np.pi / 6
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+ x[0] = x[0] % (2 * np.pi)
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+ y[0] = y[0] % (2 * np.pi)
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+
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+ return x, y
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+
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+
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+ def cohomap_vectors(
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+ cohomap_result: dict[str, Any],
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+ *,
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+ grid_size: int | None = 151,
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+ trim: int = 25,
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+ angle_grid: int = 10,
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+ phase_grid: int = 10,
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+ spacing_grid: int = 10,
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+ spacing_range: tuple[float, float] = (1.0, 6.0),
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+ ) -> dict[str, Any]:
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+ """
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+ Fit CohoMap stripe parameters and compute parallelogram vectors (v, w).
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+
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+ Returns a dict containing the stripe fit, rotated parameters, vector components,
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+ and angle (deg) following GridCellTorus conventions.
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+ """
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+ phase_map1 = np.asarray(cohomap_result["phase_map1"])
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+ phase_map2 = np.asarray(cohomap_result["phase_map2"])
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+ x_edge = np.asarray(cohomap_result["x_edge"])
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+ y_edge = np.asarray(cohomap_result["y_edge"])
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+
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+ p1, f1 = fit_cohomap_stripes(
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+ phase_map1,
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+ grid_size=grid_size,
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+ trim=trim,
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+ angle_grid=angle_grid,
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+ phase_grid=phase_grid,
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+ spacing_grid=spacing_grid,
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+ spacing_range=spacing_range,
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+ )
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+ p2, f2 = fit_cohomap_stripes(
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+ phase_map2,
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+ grid_size=grid_size,
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+ trim=trim,
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+ angle_grid=angle_grid,
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+ phase_grid=phase_grid,
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+ spacing_grid=spacing_grid,
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+ spacing_range=spacing_range,
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+ )
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+
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+ x_params, y_params = _rot_para(p1, p2)
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+
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+ x_edge_shift = x_edge - float(x_edge.min())
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+ y_edge_shift = y_edge - float(y_edge.min())
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+ xmax = float(x_edge_shift.max())
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+ ymax = float(y_edge_shift.max())
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+
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+ v = np.array(
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+ [
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+ (1 / x_params[2]) * np.cos(x_params[0]) * xmax,
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+ (1 / x_params[2]) * np.sin(x_params[0]) * xmax,
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+ ],
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+ dtype=float,
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+ )
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+ w = np.array(
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+ [
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+ (1 / y_params[2]) * np.cos(y_params[0]) * ymax,
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+ (1 / y_params[2]) * np.sin(y_params[0]) * ymax,
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+ ],
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+ dtype=float,
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+ )
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+
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+ angle_deg = float(((y_params[0] - x_params[0]) / (2 * np.pi) * 360.0) % 360.0)
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+
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+ return {
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+ "params1": p1,
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+ "params2": p2,
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+ "fit_error1": float(f1),
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+ "fit_error2": float(f2),
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+ "x_params": x_params,
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+ "y_params": y_params,
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+ "x_edge": x_edge,
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+ "y_edge": y_edge,
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+ "x_range": xmax,
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+ "y_range": ymax,
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+ "v": v,
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+ "w": w,
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+ "len_v": float(1 / x_params[2] * xmax),
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+ "len_w": float(1 / y_params[2] * ymax),
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+ "angle_deg": angle_deg,
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+ "grid_size": grid_size,
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+ "trim": trim,
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+ }
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+
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+
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+ def plot_cohomap_vectors(
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+ cohomap_vectors_result: dict[str, Any],
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+ *,
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+ config: PlotConfig | None = None,
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+ save_path: str | None = None,
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+ show: bool = False,
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+ figsize: tuple[int, int] = (5, 5),
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+ color: str = "#f28e2b",
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+ ) -> plt.Figure:
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+ """
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+ Plot v/w vectors and the parallelogram in spatial coordinates.
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+ """
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+ config = _ensure_plot_config(
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+ config,
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+ PlotConfig.for_static_plot,
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+ title="CohoMap Vectors",
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+ xlabel="",
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+ ylabel="",
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+ figsize=figsize,
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+ save_path=save_path,
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+ show=show,
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+ )
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+
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+ vmax = float(cohomap_vectors_result["x_range"])
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+ wmax = float(cohomap_vectors_result["y_range"])
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+ v = np.asarray(cohomap_vectors_result["v"], dtype=float)
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+ w = np.asarray(cohomap_vectors_result["w"], dtype=float)
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+
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+ fig, ax = plt.subplots(1, 1, figsize=config.figsize)
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+
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+ ax.plot([0, 0], [0, wmax], "--", color="0.6", lw=1)
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+ ax.plot([vmax, vmax], [0, wmax], "--", color="0.6", lw=1)
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+ ax.plot([0, vmax], [0, 0], "--", color="0.6", lw=1)
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+ ax.plot([0, vmax], [wmax, wmax], "--", color="0.6", lw=1)
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+
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+ ax.plot([0, v[0]], [0, v[1]], color=color, lw=3)
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+ ax.plot([0, w[0]], [0, w[1]], color=color, lw=3)
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+ ax.plot([v[0], v[0] + w[0]], [v[1], v[1] + w[1]], color=color, lw=3)
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+ ax.plot([w[0], v[0] + w[0]], [w[1], v[1] + w[1]], color=color, lw=3)
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+
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+ pad_x = 0.05 * vmax if vmax > 0 else 1.0
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+ pad_y = 0.05 * wmax if wmax > 0 else 1.0
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+ ax.set_xlim(-pad_x, vmax + pad_x)
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+ ax.set_ylim(-pad_y, wmax + pad_y)
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+ ax.set_aspect("equal", "box")
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+ ax.set_xticks([])
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+ ax.set_yticks([])
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+
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+ fig.tight_layout()
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+ _ensure_parent_dir(config.save_path)
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+ finalize_figure(fig, config)
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+ return fig
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+
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+
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+ def _resolve_grid_size(phase_map: np.ndarray, grid_size: int | None, trim: int) -> int:
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+ if grid_size is None:
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+ return int(phase_map.shape[0]) + 2 * trim + 1
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+ return int(grid_size)
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+
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+
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+ def _stripe_fit_map(params: np.ndarray, grid_size: int, trim: int) -> np.ndarray:
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+ numangsint = grid_size
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+ x, _ = np.meshgrid(
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+ np.linspace(0, 3 * np.pi, numangsint - 1),
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+ np.linspace(0, 3 * np.pi, numangsint - 1),
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+ )
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+ x_rot = rotate(x, params[0] * 360.0 / (2 * np.pi), reshape=False)
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+ return np.cos(params[2] * x_rot[trim:-trim, trim:-trim] + params[1])
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+
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+
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+ def plot_cohomap_stripes(
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+ cohomap_result: dict[str, Any],
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+ *,
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+ cohomap_vectors_result: dict[str, Any] | None = None,
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+ grid_size: int | None = 151,
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+ trim: int = 25,
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+ angle_grid: int = 10,
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+ phase_grid: int = 10,
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+ spacing_grid: int = 10,
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+ spacing_range: tuple[float, float] = (1.0, 6.0),
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+ config: PlotConfig | None = None,
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+ save_path: str | None = None,
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+ show: bool = False,
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+ figsize: tuple[int, int] = (10, 6),
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+ cmap: str = "viridis",
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+ ) -> plt.Figure:
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+ """
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+ Plot stripe fit diagnostics for CohoMap (observed vs fitted stripes).
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+ """
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+ if cohomap_vectors_result is None:
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+ cohomap_vectors_result = cohomap_vectors(
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+ cohomap_result,
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+ grid_size=grid_size,
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+ trim=trim,
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+ angle_grid=angle_grid,
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+ phase_grid=phase_grid,
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+ spacing_grid=spacing_grid,
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+ spacing_range=spacing_range,
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+ )
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+
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+ if "grid_size" in cohomap_vectors_result:
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+ grid_size = cohomap_vectors_result["grid_size"]
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+ if "trim" in cohomap_vectors_result:
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+ trim = cohomap_vectors_result["trim"]
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+
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+ config = _ensure_plot_config(
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+ config,
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+ PlotConfig.for_static_plot,
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+ title="CohoMap Stripe Fit",
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+ xlabel="",
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+ ylabel="",
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+ figsize=figsize,
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+ save_path=save_path,
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+ show=show,
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+ )
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+
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+ phase_map1 = np.asarray(cohomap_result["phase_map1"])
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+ phase_map2 = np.asarray(cohomap_result["phase_map2"])
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+ x_edge = np.asarray(cohomap_result["x_edge"])
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+ y_edge = np.asarray(cohomap_result["y_edge"])
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+
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+ grid_size = _resolve_grid_size(phase_map1, grid_size, trim)
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+ expected = grid_size - 1 - 2 * trim
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+ if phase_map1.shape[0] != expected or phase_map2.shape[0] != expected:
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+ raise ValueError("phase_map shape does not match grid_size/trim")
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+
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+ p1 = np.asarray(cohomap_vectors_result["params1"])
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+ p2 = np.asarray(cohomap_vectors_result["params2"])
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+ fit1 = _stripe_fit_map(p1, grid_size, trim)
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+ fit2 = _stripe_fit_map(p2, grid_size, trim)
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+
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+ obs1 = np.cos(phase_map1)
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+ obs2 = np.cos(phase_map2)
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+
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+ fig, axes = plt.subplots(2, 2, figsize=config.figsize)
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+ panels = [
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+ (obs1, "Phase Map 1 (cos)"),
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+ (fit1, "Stripe Fit 1"),
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+ (obs2, "Phase Map 2 (cos)"),
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+ (fit2, "Stripe Fit 2"),
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+ ]
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+
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+ for ax, (img, title) in zip(axes.flat, panels, strict=True):
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+ ax.imshow(
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+ img,
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+ origin="lower",
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+ extent=[x_edge[0], x_edge[-1], y_edge[0], y_edge[-1]],
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+ cmap=cmap,
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+ vmin=-1.0,
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+ vmax=1.0,
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+ )
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+ ax.set_title(title, fontsize=10)
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+ ax.set_aspect("equal", "box")
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+ ax.set_xticks([])
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+ ax.set_yticks([])
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+
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+ fig.tight_layout()
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+ _ensure_parent_dir(config.save_path)
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+ finalize_figure(fig, config)
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+ return fig