biopipen 0.34.1__py3-none-any.whl → 0.34.2__py3-none-any.whl
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
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- biopipen/__init__.py +1 -1
- biopipen/ns/scrna.py +243 -31
- biopipen/ns/tcr.py +4 -4
- biopipen/scripts/scrna/MarkersFinder.R +34 -28
- biopipen/scripts/scrna/PseudoBulkDEG.R +592 -0
- biopipen/scripts/scrna/ScFGSEA.R +35 -35
- biopipen/scripts/scrna/TopExpressingGenes.R +6 -6
- biopipen/scripts/tcr/ScRepCombiningExpression.R +2 -2
- {biopipen-0.34.1.dist-info → biopipen-0.34.2.dist-info}/METADATA +1 -1
- {biopipen-0.34.1.dist-info → biopipen-0.34.2.dist-info}/RECORD +12 -12
- biopipen/reports/scrna/TopExpressingGenes.svelte +0 -17
- {biopipen-0.34.1.dist-info → biopipen-0.34.2.dist-info}/WHEEL +0 -0
- {biopipen-0.34.1.dist-info → biopipen-0.34.2.dist-info}/entry_points.txt +0 -0
biopipen/scripts/scrna/ScFGSEA.R
CHANGED
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@@ -7,9 +7,9 @@ srtfile <- {{in.srtobj | r}} # nolint
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outdir <- {{out.outdir | r}} # nolint
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joboutdir <- {{job.outdir | r}} # nolint
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mutaters <- {{envs.mutaters | r}} # nolint
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group_by <- {{envs.group_by | default: envs["group-by"] | default: None | r}} # nolint
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ident_1 <- {{envs.ident_1 | default: envs["ident-1"] | default: None | r}} # nolint
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ident_2 <- {{envs.ident_2 | default: envs["ident-2"] | default: None | r}} # nolint
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each <- {{envs.each | r}} # nolint
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subset <- {{envs.subset | r}} # nolint
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gmtfile <- {{envs.gmtfile | r}} # nolint
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@@ -18,8 +18,8 @@ top <- {{envs.top | r}} # nolint
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minsize <- {{envs.minSize | default: envs.minsize | r}} # nolint
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maxsize <- {{envs.maxSize | default: envs.maxsize | r}} # nolint
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eps <- {{envs.eps | r}} # nolint
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-
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-
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alleach_plots_defaults <- {{envs.alleach_plots_defaults | r}} # nolint
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alleach_plots <- {{envs.alleach_plots | r}} #
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ncores <- {{envs.ncores | r}} # nolint
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rest <- {{envs.rest | r: todot="-"}} # nolint
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cases <- {{envs.cases | r: todot="-"}} # nolint
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@@ -27,8 +27,8 @@ cases <- {{envs.cases | r: todot="-"}} # nolint
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log <- get_logger()
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reporter <- get_reporter()
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-
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list_update(
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alleach_plots <- lapply(alleach_plots, function(x) {
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list_update(alleach_plots_defaults, x)
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})
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log$info("Reading Seurat object ...")
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@@ -43,9 +43,9 @@ if (!is.null(mutaters) && length(mutaters) > 0) {
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}
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defaults <- list(
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group_by = group_by,
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ident_1 = ident_1,
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ident_2 = ident_2,
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each = each,
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subset = subset,
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gmtfile = gmtfile,
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@@ -54,8 +54,8 @@ defaults <- list(
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minsize = minsize,
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maxsize = maxsize,
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eps = eps,
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alleach_plots_defaults = alleach_plots_defaults,
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alleach_plots = alleach_plots,
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ncores = ncores,
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rest = rest
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)
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@@ -63,11 +63,11 @@ defaults <- list(
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expand_each <- function(name, case) {
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outcases <- list()
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case$
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case$group_by <- case$group_by %||% "Identity"
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if (is.null(case$each) || is.na(case$each) || nchar(case$each) == 0 || isFALSE(each)) {
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if (length(case$
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stop("Cannot perform `
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if (length(case$alleach_plots) > 0) {
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stop("Cannot perform `alleach_plots` without `each` defined.")
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}
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outcases[[name]] <- case
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newcase$each_name <- case$each
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newcase$each <- each
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newcase$
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newcase$
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newcase$alleach_plots_defaults <- NULL
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newcase$alleach_plots <- NULL
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if (!is.null(case$subset)) {
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newcase$subset <- paste0(case$subset, " & ", bQuote(case$each), " == '", each, "'")
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outcases[[newname]] <- newcase
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}
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if (length(case$
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if (length(case$alleach_plots) > 0) {
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newcase <- case
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newcase$gseas <- list()
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newcase$
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newcase$
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function(x) { list_update(newcase$
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newcase$alleach_plots <- lapply(
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newcase$alleach_plots,
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function(x) { list_update(newcase$alleach_plots_defaults, x) }
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)
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outcases[[paste0(name, " (all ", case$each,")")]] <- newcase
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}))
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gseas[[case$each]] <- factor(gseas[[case$each]], levels = each_levels)
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for (plotname in names(case$
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plotargs <- case$
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for (plotname in names(case$alleach_plots)) {
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plotargs <- case$alleach_plots[[plotname]]
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plotargs <- extract_vars(plotargs, "devpars")
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plotargs$gsea_results <- gseas
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plotargs$group_by <- case$each
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allow_empty = !is.null(case$each)
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# prepare expression matrix
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log$info(" Preparing expression matrix...")
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sobj <- ensure_sobj({ srtobj %>% filter(!is.na(!!sym(case$
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sobj <- ensure_sobj({ srtobj %>% filter(!is.na(!!sym(case$group_by))) }, allow_empty)
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if (is.null(sobj)) {
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reporter$add2(
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list(
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kind = "error",
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content = paste0("No cells with non-NA `", case$
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content = paste0("No cells with non-NA `", case$group_by, "` in the Seurat object.")
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),
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hs = c(info$section, info$name)
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)
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reporter$add2(
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list(
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kind = "error",
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content = paste0("No cells with non-NA `", case$
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content = paste0("No cells with non-NA `", case$group_by, "` in the Seurat object.")
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),
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hs = c(info$section, info$name)
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)
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return(NULL)
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}
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}
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if (!is.null(case$
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sobj <- ensure_sobj({ sobj %>% filter(!!sym(case$
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if (!is.null(case$ident_2)) {
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sobj <- ensure_sobj({ sobj %>% filter(!!sym(case$group_by) %in% c(case$ident_1, case$ident_2)) }, allow_empty)
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if (is.null(sobj)) {
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reporter$add2(
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list(
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kind = "error",
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content = paste0("No cells with non-NA `", case$
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content = paste0("No cells with non-NA `", case$group_by, "` in the Seurat object.")
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),
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hs = c(info$section, info$name)
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)
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}
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}
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allclasses <- sobj@meta.data[, case$
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if (is.null(case$
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case$
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allclasses[allclasses != case$
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allclasses <- sobj@meta.data[, case$group_by, drop = TRUE]
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if (is.null(case$ident_2)) {
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case$ident_2 <- "Other"
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allclasses[allclasses != case$ident_1] <- "Other"
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}
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exprs <- GetAssayData(sobj, layer = "data")
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# get preranks
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log$info(" Getting preranks...")
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ranks <- RunGSEAPreRank(exprs, allclasses, case$
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ranks <- RunGSEAPreRank(exprs, allclasses, case$ident_1, case$ident_2, case$method)
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write.table(
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as.data.frame(ranks),
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file.path(info$prefix, "fgsea.rank.txt"),
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reporter$add2(
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list(
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name = paste0("Table (", case$
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name = paste0("Table (", case$ident_1, " vs ", case$ident_2, ")"),
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contents = list(
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list(kind = "descr", content = paste0(
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"Showing top 50 pathways by padj in descending order. ",
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joboutdir <- {{job.outdir | r}}
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mutaters <- {{ envs.mutaters | r }}
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ident <- {{ envs.ident | r }}
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group_by <- {{ envs.group_by | default: envs["group-by"] | default: None | r }} # nolint
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each <- {{ envs.each | r }}
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dbs <- {{ envs.dbs | r }}
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n <- {{ envs.n | r }}
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defaults <- list(
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ident = ident,
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group_by = group_by,
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each = each,
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dbs = dbs,
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n = n,
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} else {
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subobj <- srtobj
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}
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case$
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case$group_by <- case$group_by %||% "Identity"
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if (is.null(case$ident)) {
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case$ident <- as.character(unique(subobj@meta.data[[case$
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case$ident <- as.character(unique(subobj@meta.data[[case$group_by]]))
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}
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avgexpr <- AverageExpression(
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subobj,
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group_by = case$group_by,
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assays = assay
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)[[assay]]
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# https://github.com/satijalab/seurat/issues/7893
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colnames(avgexpr) <- as.character(unique(subobj@meta.data[[case$
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colnames(avgexpr) <- as.character(unique(subobj@meta.data[[case$group_by]]))
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avgexpr <- avgexpr[, case$ident, drop = FALSE]
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for (idt in case$ident) {
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@@ -7,7 +7,7 @@ srtobjfile <- {{in.srtobj | r}}
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outfile <- {{out.outfile | r}}
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cloneCall <- {{envs.cloneCall | r}}
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chain <- {{envs.chain | r}}
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group_by <- {{envs.group_by | default: envs["group-by"] | default: None | r}}
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proportion <- {{envs.proportion | r}}
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filterNA <- {{envs.filterNA | r}}
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cloneSize <- {{envs.cloneSize | r}}
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sc.data = srtobj,
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cloneCall = cloneCall,
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chain = chain,
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group.by =
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group.by = group_by,
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proportion = proportion,
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filterNA = filterNA,
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cloneSize = unlist(cloneSize),
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biopipen/__init__.py,sha256=
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biopipen/__init__.py,sha256=NtTwQ_23yhWzaTLoJ9fdSclacR1T9pyG3ntupTpaz6g,23
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biopipen/core/__init__.py,sha256=47DEQpj8HBSa-_TImW-5JCeuQeRkm5NMpJWZG3hSuFU,0
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biopipen/core/config.py,sha256=edK5xnDhM8j27srDzsxubi934NMrglLoKrdcC8qsEPk,1069
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biopipen/core/config.toml,sha256=lZV_vbYWk6uqm19ZWJcsZCcSNqAdIfN2fOfamzxZpg4,2148
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@@ -22,12 +22,12 @@ biopipen/ns/plot.py,sha256=N41_izb6zi-XArUly5WhLebapNXbTNSgGlOCCwtrDlY,18282
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biopipen/ns/protein.py,sha256=YJtlKoHI2p5yHdxKeQnNtm5QrbxDGOq1UXOdt_7tlTs,6391
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biopipen/ns/regulatory.py,sha256=gJjGVpJrdv-rg2t5UjK4AGuvtLNymaNYNvoD8PhlbvE,15929
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biopipen/ns/rnaseq.py,sha256=bKAa6friFWof4yDTWZQahm1MS-lrdetO1GqDKdfxXYc,7708
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biopipen/ns/scrna.py,sha256=
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biopipen/ns/scrna.py,sha256=1z-PUPkQ6FpfC8RHWtuG8Mvw7w6zmI-Z3shkzO8cekY,143767
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biopipen/ns/scrna_metabolic_landscape.py,sha256=Q95KkHg5jC6eUMSUH-wioPxOzuArP59j3CPsfDTCBM0,22096
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biopipen/ns/snp.py,sha256=iXWrw7Lmhf4_ct57HGT7JGTClCXUD4sZ2FzOgsC2pTg,28123
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biopipen/ns/stats.py,sha256=DlPyK5Vsg6ZEkV9SDS3aAw21eXzvOHgqeZDkXPhg7go,20509
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biopipen/ns/tcgamaf.py,sha256=AFbUJIxiMSvsVY3RcHgjRFuMnNh2DG3Mr5slLNEyz6o,1455
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biopipen/ns/tcr.py,sha256=
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biopipen/ns/tcr.py,sha256=dw6zEm5FNzf5ViVNxX81YwlzmXSAwWszxk6OL4Yh5p0,99036
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biopipen/ns/vcf.py,sha256=zjOH2qiSJsHACLmBqV-Tew-mn-peZgvYLAWjTLh7uTI,23823
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biopipen/ns/web.py,sha256=8VY4Xsb8UrzS4IkGUX_84GQP1JG6NcTZrV7f9tA1tUI,5458
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biopipen/reports/bam/CNAClinic.svelte,sha256=D4IxQcgDCPQZMbXog-aZP5iJEQTK2N4i0C60e_iXyfs,213
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@@ -51,7 +51,6 @@ biopipen/reports/scrna/DimPlots.svelte,sha256=ubIx8dgppzSB8WS_B4LN2T3bOTerP4Ck6o
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biopipen/reports/scrna/MarkersFinder.svelte,sha256=77rD1psj0VJykPDhfwS-B8mubvaasREAE6RYR2atTN4,444
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biopipen/reports/scrna/MetaMarkers.svelte,sha256=iIFRKjvVYrM1AtDWqq8UfeS8q23R8FKg2yepKAw2KSE,508
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biopipen/reports/scrna/RadarPlots.svelte,sha256=g_fp9d3vdnzk-egXPhkhhfWXOeG569Rj8rYLRIKmlLc,396
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biopipen/reports/scrna/TopExpressingGenes.svelte,sha256=tt5_Vjym4coFT8Bvz0s6ZcCioTOIwCj83jdCGqPCmUw,491
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biopipen/reports/scrna_metabolic_landscape/MetabolicFeatures.svelte,sha256=1RC-FuYr_M1xInPaNrEGyzPQGy2d1rZjYdKPfLAOPUs,2346
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biopipen/reports/scrna_metabolic_landscape/MetabolicPathwayActivity.svelte,sha256=VTU-D8iELO7zzK5cJg7oZTna2wu4O_gJ8d7G8N7Veg8,5473
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biopipen/reports/scrna_metabolic_landscape/MetabolicPathwayHeterogeneity.svelte,sha256=Fr9_DJPuv2hzac-zzqtVBir-FXwN-g2fyi7Le_7xfPs,2828
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biopipen/scripts/scrna/ExprImputation-scimpute.R,sha256=MI_bYfvCDKJsuGntUxfx_-NdrssBoQgL95-DGwJVE5s,1191
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biopipen/scripts/scrna/ExprImputation.R,sha256=GcdZJpkDpq88hRQjtLZY5-byp8V43stEFm5T-pQbU6A,319
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biopipen/scripts/scrna/LoomTo10X.R,sha256=c6F0p1udsL5UOlb84-53K5BsjSDWkdFyYTt5NQmlIec,1059
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biopipen/scripts/scrna/MarkersFinder.R,sha256=
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biopipen/scripts/scrna/MarkersFinder.R,sha256=P6BgseCrXTeJR8X52hzD16qBUuCeHmPc96h5pKE_-qY,24207
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biopipen/scripts/scrna/MetaMarkers.R,sha256=BgYaWYEj6obwqaZaDWqNPtxb1IEEAnXAeBE0Ji9PvBA,12426
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biopipen/scripts/scrna/ModuleScoreCalculator.R,sha256=-tByCPk7i070LynAb0z2ANeRxr1QqiKP0dfrJm52jH4,4198
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biopipen/scripts/scrna/PseudoBulkDEG.R,sha256=32Hd3x2WyTFv175Os4bxf6goAcIq7QN8m1i7i7emnMI,22308
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biopipen/scripts/scrna/RadarPlots.R,sha256=Kn1E-hpczuujpgNjR8MqeIIVN-S3PbpmfcKWGKcNCVY,14546
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biopipen/scripts/scrna/SCImpute.R,sha256=dSJOHhmJ3x_72LBRXT72dbCti5oiB85CJ-OjWtqONbk,2958
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biopipen/scripts/scrna/SCP-plot.R,sha256=QcR2zOjRlSA_z4L8l89FWPU7TGxpXlKUe4kPdZU9MuY,787291
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biopipen/scripts/scrna/ScSimulation.R,sha256=q0-dXD9px1cApc_TxGmR-OdNHE8W1VSVWfSI57B96bo,1697
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biopipen/scripts/scrna/ScVelo.py,sha256=SPUZFgZW1Zhw-bnjJo98RK0vpuNFODQ8Q3eTguNc84k,21359
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biopipen/scripts/scrna/Seurat2AnnData.R,sha256=F8g5n2CqX4-KBggxd8ittz8TejYuqqNLMudAHdFt1QM,184
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biopipen/scripts/scrna/Slingshot.R,sha256=wo1zq2Wl6u1HODNzZGjjQLcqKeh9sh7FXPs_iKu6tqw,1750
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biopipen/scripts/scrna/Subset10X.R,sha256=dT1QY5mHaDcqOMgAtTfyU1FRBNFtfg3nMGCubvBJcSQ,2671
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biopipen/scripts/scrna/celltypist-wrapper.py,sha256=upyh035IqDHxljbTaoXwdDmctcx-fDwN56kGvC2xsbw,1776
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biopipen/scripts/scrna/sctype.R,sha256=NaUJkABwF5G1UVm1CCtcMbwLSj94Mo24mbYCKFqo1Bw,6524
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biopipen/scripts/scrna/seurat_anndata_conversion.py,sha256=Ya0Wn2TLg1j66N41PdiXXGE8LtE51eC8XnkGi_q2ey8,2437
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biopipen/scripts/tcr/ImmunarchSplitIdents.R,sha256=FGCeGV0uSmFU91lKkldUAeV4A2m3hHw5X4GNi8ffGzI,1873
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biopipen/scripts/tcr/SampleDiversity.R,sha256=oipN4-2nQZe8bYjI0lZ0SvZ7T8GZ_FWkpkobi1cwmWE,2664
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biopipen/scripts/tcr/ScRepLoading.R,sha256=eqJXTXn_HyLRo98Hv6xI_AmYp5l2LxHsyrMSXWZ_HmM,5167
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biopipen/scripts/tcr/TCRClusterStats.R,sha256=ns3S95DVDBuhSe1YgTZ1OksbfBgREO2Tnp1d4QzbTw0,13530
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biopipen/scripts/tcr/TCRClustering.R,sha256=b-IZeE3Rcue4Ntn9H7FLljELzycbWCyKP17LlBCuMW0,9658
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biopipen/utils/reporter.py,sha256=VwLl6xyVDWnGY7NEXyqBlkW8expKJoNQ5iTyZSELf5c,4922
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{% from "utils/misc.liq" import report_jobs -%}
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{% from "utils/gsea.liq" import enrichr_report -%}
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