biopipen 0.23.7__py3-none-any.whl → 0.24.0__py3-none-any.whl
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- biopipen/__init__.py +1 -1
- biopipen/core/proc.py +7 -0
- biopipen/ns/cellranger.py +2 -2
- biopipen/ns/scrna.py +15 -20
- biopipen/ns/tcr.py +8 -6
- biopipen/scripts/scrna/ScFGSEA.R +6 -0
- biopipen/scripts/scrna/SeuratClustering.R +102 -85
- biopipen/scripts/scrna/SeuratPreparing.R +21 -10
- biopipen/scripts/scrna/SeuratSubClustering.R +81 -97
- biopipen/scripts/scrna_metabolic_landscape/MetabolicFeaturesIntraSubset.R +5 -5
- biopipen/scripts/scrna_metabolic_landscape/MetabolicPathwayActivity.R +3 -3
- biopipen/scripts/tcr/Immunarch-basic.R +2 -3
- biopipen/scripts/tcr/Immunarch-clonality.R +2 -3
- biopipen/scripts/tcr/Immunarch-diversity.R +70 -49
- biopipen/scripts/tcr/Immunarch-geneusage.R +2 -3
- biopipen/scripts/tcr/Immunarch-kmer.R +2 -3
- biopipen/scripts/tcr/Immunarch-overlap.R +2 -3
- biopipen/scripts/tcr/Immunarch-spectratyping.R +2 -3
- biopipen/scripts/tcr/Immunarch-tracking.R +2 -3
- biopipen/scripts/tcr/Immunarch-vjjunc.R +2 -3
- biopipen/scripts/tcr/Immunarch.R +1 -1
- biopipen/scripts/tcr/ImmunarchLoading.R +2 -0
- biopipen/scripts/tcr/TCRClustering.R +6 -2
- biopipen/scripts/tcr/TESSA.R +3 -1
- biopipen/scripts/tcr/immunarch-patched.R +142 -0
- biopipen/utils/caching.R +44 -0
- {biopipen-0.23.7.dist-info → biopipen-0.24.0.dist-info}/METADATA +8 -7
- {biopipen-0.23.7.dist-info → biopipen-0.24.0.dist-info}/RECORD +30 -28
- {biopipen-0.23.7.dist-info → biopipen-0.24.0.dist-info}/WHEEL +0 -0
- {biopipen-0.23.7.dist-info → biopipen-0.24.0.dist-info}/entry_points.txt +0 -0
biopipen/scripts/tcr/TESSA.R
CHANGED
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library(immunarch)
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vis.immunr_gini <- function(.data, .by = NA, .meta = NA,
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.errorbars = c(0.025, 0.975), .errorbars.off = FALSE,
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.points = TRUE, .test = TRUE, .signif.label.size = 3.5,
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.legend = NA, .plot.type = "bar", ...) {
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# repDiversity(..., .method = "gini") generates a matrix
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.data = data.frame(Sample = rownames(.data), Value = .data[, 1])
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if (.plot.type == "bar") {
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vis_bar(
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.data = .data, .by = .by, .meta = .meta,
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.errorbars = .errorbars, .errorbars.off = .errorbars.off, .stack = FALSE,
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.points = .points, .test = .test, .signif.label.size = .signif.label.size,
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.defgroupby = "Sample", .grouping.var = "Group",
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.labs = c(NA, "Gini coefficient"),
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.title = "Gini coefficient", .subtitle = "Sample diversity estimation using the Gini coefficient",
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.legend = .legend, .leg.title = NA
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)
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} else {
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vis_box(
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.data = .data, .by = .by, .meta = .meta, .test = .test,
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.points = .points, .signif.label.size = .signif.label.size,
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.defgroupby = "Sample", .grouping.var = "Group",
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.labs = c(NA, "Gini coefficient"),
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.title = "Gini coefficient", .subtitle = "Sample diversity estimation using the Gini coefficient",
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.legend = .legend, .leg.title = NA, .melt = FALSE
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)
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}
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}
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vis.immunr_div <- function(.data, .by = NA, .meta = NA,
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.errorbars = c(0.025, 0.975), .errorbars.off = FALSE,
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.points = TRUE, .test = TRUE, .signif.label.size = 3.5,
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.legend = NA, .plot.type = "bar", ...) {
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# repDiversity(..., .method = "gini") generates a matrix
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if (.plot.type == "bar") {
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immunarch:::vis.immunr_div(.data = .data,.by = .by, .meta = .meta,
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.errorbars = .errorbars, .errorbars.off = .errorbars.off, .stack = FALSE,
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.points = .points, .test = .test, .signif.label.size = .signif.label.size,
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.legend = .legend)
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} else {
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vis_box(
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.data = .data, .by = .by, .meta = .meta, .test = .test,
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.points = .points, .signif.label.size = .signif.label.size,
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.defgroupby = "Sample", .grouping.var = "Group",
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.labs = c(NA, "Effective number of clonoypes"),
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.title = "True diversity", .subtitle = "Sample diversity estimation using the true diversity index",
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.legend = NA, .leg.title = NA, .melt = FALSE
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)
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}
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}
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vis.immunr_chao1 <- function(.data, .by = NA, .meta = NA,
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.errorbars = c(0.025, 0.975), .errorbars.off = FALSE,
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.points = TRUE, .test = TRUE, .signif.label.size = 3.5,
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.legend = NA, .plot.type = "bar", ...) {
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# repDiversity(..., .method = "gini") generates a matrix
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if (.plot.type == "bar") {
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immunarch:::vis.immunr_chao1(.data = .data,.by = .by, .meta = .meta,
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.errorbars = .errorbars, .errorbars.off = .errorbars.off, .stack = FALSE,
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.points = .points, .test = .test, .signif.label.size = .signif.label.size,
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.legend = .legend)
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} else {
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.data <- data.frame(Sample = row.names(.data), Value = .data[, 1])
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vis_box(
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.data = .data, .by = .by, .meta = .meta, .test = .test,
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.points = .points, .signif.label.size = .signif.label.size,
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.defgroupby = "Sample", .grouping.var = "Group",
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.labs = c(NA, "Chao1"),
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.title = "Chao1", .subtitle = "Sample diversity estimation using Chao1",
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.legend = NA, .leg.title = NA, .melt = FALSE
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)
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}
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}
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vis.immunr_ginisimp <- function(.data, .by = NA, .meta = NA,
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.errorbars = c(0.025, 0.975), .errorbars.off = FALSE,
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.points = TRUE, .test = TRUE, .signif.label.size = 3.5,
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.legend = NA, .plot.type = "bar", ...) {
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# repDiversity(..., .method = "gini") generates a matrix
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if (.plot.type == "bar") {
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immunarch:::vis.immunr_ginisimp(.data = .data,.by = .by, .meta = .meta,
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.errorbars = .errorbars, .errorbars.off = .errorbars.off, .stack = FALSE,
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.points = .points, .test = .test, .signif.label.size = .signif.label.size,
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.legend = .legend)
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} else {
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vis_box(
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.data = .data, .by = .by, .meta = .meta, .test = .test,
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.points = .points, .signif.label.size = .signif.label.size,
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.defgroupby = "Sample", .grouping.var = "Group",
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.labs = c(NA, "Gini-Simpson index"),
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.title = "Gini-Simpson index", .subtitle = "Sample diversity estimation using the Gini-Simpson index",
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.legend = .legend, .leg.title = NA, .melt = FALSE
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)
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}
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}
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vis.immunr_invsimp <- function(.data, .by = NA, .meta = NA,
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.errorbars = c(0.025, 0.975), .errorbars.off = FALSE,
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.points = TRUE, .test = TRUE, .signif.label.size = 3.5,
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.legend = NA, .plot.type = "bar", ...) {
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# repDiversity(..., .method = "gini") generates a matrix
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if (.plot.type == "bar") {
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immunarch:::vis.immunr_invsimp(.data = .data,.by = .by, .meta = .meta,
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.errorbars = .errorbars, .errorbars.off = .errorbars.off, .stack = FALSE,
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.points = .points, .test = .test, .signif.label.size = .signif.label.size,
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.legend = .legend)
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} else {
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vis_box(
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.data = .data, .by = .by, .meta = .meta, .test = .test,
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.points = .points, .signif.label.size = .signif.label.size,
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.defgroupby = "Sample", .grouping.var = "Group",
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.labs = c(NA, "Inverse Simpson index"),
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.title = "Inverse Simpson index", .subtitle = "Sample diversity estimation using the inverse Simpson index",
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.legend = .legend, .leg.title = NA, .melt = FALSE
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)
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}
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}
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vis.immunr_dxx <- function(.data, .by = NA, .meta = NA,
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.errorbars = c(0.025, 0.975), .errorbars.off = FALSE,
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.points = TRUE, .test = TRUE, .signif.label.size = 3.5,
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.legend = NA, .plot.type = "bar", ...) {
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# repDiversity(..., .method = "gini") generates a matrix
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if (.plot.type == "bar") {
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immunarch:::vis.immunr_dxx(.data = .data,.by = .by, .meta = .meta,
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.errorbars = .errorbars, .errorbars.off = .errorbars.off, .stack = FALSE,
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.points = .points, .test = .test, .signif.label.size = .signif.label.size,
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.legend = .legend)
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} else {
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perc_value <- round(.data[1, 2][1])
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.data <- data.frame(Sample = row.names(.data), Value = .data[, 1])
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vis_box(
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.data = .data, .by = .by, .meta = .meta, .test = .test,
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.points = .points, .signif.label.size = .signif.label.size,
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.defgroupby = "Sample", .grouping.var = "Group",
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.labs = c(NA, paste0("D", perc_value)),
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.title = paste0("D", perc_value, " diversity index"), .subtitle = paste0("Number of clonotypes occupying the ", perc_value, "% of repertoires"),
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.legend = .legend, .leg.title = NA, .melt = FALSE
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)
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}
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}
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biopipen/utils/caching.R
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library(digest)
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#' Get signatures and cached data
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#'
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#' @param x An object to infer signature from
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#' @param kind A string indicating the kind of the object
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#' Used as part of the filename of the cached file
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#' @param cache_dir A string indicating the directory to store cached files
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#'
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#' @return A list containing the signature, digested signature and cached data
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get_cached <- function(x, kind, cache_dir) {
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if (is.null(cache_dir) || isFALSE(cache_dir)) {
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return(list(sig = NULL, dig = NULL, data = NULL))
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}
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# Get signature of an object
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sig <- capture.output(str(x))
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dig <- digest::digest(sig, algo = "md5")
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dig <- substr(dig, 1, 8)
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cached_file <- file.path(cache_dir, paste0(dig, ".", kind, ".RDS"))
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if (!file.exists(cached_file)) {
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return(list(sig = sig, dig = dig, data = NULL))
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}
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list(sig = sig, dig = dig, data = readRDS(cached_file))
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}
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#' Save an object to cache
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#'
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#' @param to_cache An list to cache,
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#' including the signature, digested signature and data
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#' @param kind A string indicating the kind of the object
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#' Used as part of the filename of the cached file
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#' @param cache_dir A string indicating the directory to store cached files
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save_to_cache <- function(to_cache, kind, cache_dir) {
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if (is.null(cache_dir) || isFALSE(cache_dir)) { return() }
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dig <- to_cache$dig
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sig <- to_cache$sig
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data <- to_cache$data
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# Save an object to cache
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sig_file <- file.path(cache_dir, paste0(dig, ".", kind , ".signature.txt"))
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writeLines(c(as.character(Sys.time()), "", sig), sig_file)
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cached_file <- file.path(cache_dir, paste0(dig, ".", kind, ".RDS"))
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saveRDS(data, cached_file)
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}
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Metadata-Version: 2.1
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Name: biopipen
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Version: 0.
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Version: 0.24.0
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Summary: Bioinformatics processes/pipelines that can be run from `pipen run`
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License: MIT
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Author: pwwang
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Classifier: Programming Language :: Python :: 3.11
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Classifier: Programming Language :: Python :: 3.12
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Provides-Extra: runinfo
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Requires-Dist: datar[pandas] (>=0.15.
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Requires-Dist: datar[pandas] (>=0.15.3,<0.16.0)
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Requires-Dist: pipen-board[report] (>=0.14,<0.15)
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Requires-Dist: pipen-cli-run (>=0.12,<0.13)
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Requires-Dist: pipen-filters (>=0.11,<0.12)
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Requires-Dist: pipen-poplog (>=0.0.2,<0.0.3)
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Requires-Dist: pipen-runinfo (>=0.5,<0.6) ; extra == "runinfo"
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Requires-Dist: pipen-verbose (>=0.10,<0.11)
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biopipen/__init__.py,sha256=
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biopipen/__init__.py,sha256=DxtMZD542lg_xb6icrE2d5JOY8oUi-v34i2Ar63ddvs,23
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biopipen/core/__init__.py,sha256=47DEQpj8HBSa-_TImW-5JCeuQeRkm5NMpJWZG3hSuFU,0
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biopipen/core/config.py,sha256=edK5xnDhM8j27srDzsxubi934NMrglLoKrdcC8qsEPk,1069
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biopipen/core/config.toml,sha256=Rn7Cta7WsMtmQkKGC4h9d5dU_STaIVBgR8UliiGgL6o,1757
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biopipen/core/defaults.py,sha256=yPeehPLk_OYCf71IgRVCWuQRxLAMixDF81Ium0HtPKI,344
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biopipen/core/filters.py,sha256=HLrjXGsvvjRtTWIAmg_f4IMymWaRD769HlDwsCTh170,12424
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biopipen/core/proc.py,sha256=
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biopipen/core/proc.py,sha256=60lUP3PcUAaKbDETo9N5PEIoeOYrLgcSmuytmrhcx8g,912
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biopipen/core/testing.py,sha256=5vR15kkCjfXM7Bx0HBzabNLtDLAEX4uU94TskCkPni8,1447
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biopipen/ns/__init__.py,sha256=47DEQpj8HBSa-_TImW-5JCeuQeRkm5NMpJWZG3hSuFU,0
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biopipen/ns/bam.py,sha256=5AsYrB0mtr_mH6mCL6gjJ5rC4NywpjFkpFjUrBGp7Fk,9301
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