biopipen 0.22.0__py3-none-any.whl → 0.22.2__py3-none-any.whl
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- biopipen/__init__.py +1 -1
- biopipen/core/config.toml +6 -0
- biopipen/core/filters.py +12 -0
- biopipen/ns/cellranger.py +101 -0
- biopipen/ns/scrna.py +2 -0
- biopipen/ns/tcr.py +30 -10
- biopipen/reports/cellranger/CellRangerCount.svelte +16 -0
- biopipen/reports/cellranger/CellRangerVdj.svelte +16 -0
- biopipen/scripts/cellranger/CellRangerCount.py +79 -0
- biopipen/scripts/cellranger/CellRangerVdj.py +79 -0
- biopipen/scripts/scrna/CellTypeAnnotation-direct.R +31 -24
- biopipen/scripts/scrna/CellsDistribution.R +9 -8
- biopipen/scripts/scrna/MarkersFinder.R +106 -28
- biopipen/scripts/scrna/SeuratClusterStats-features.R +2 -2
- biopipen/scripts/scrna/SeuratMetadataMutater.R +13 -1
- biopipen/scripts/tcr/Attach2Seurat.R +2 -1
- biopipen/scripts/tcr/CDR3AAPhyschem.R +1 -1
- biopipen/scripts/tcr/Immunarch.R +3 -0
- biopipen/scripts/tcr/ImmunarchLoading.R +22 -23
- biopipen/scripts/tcr/TCRClustering.R +8 -9
- biopipen/scripts/tcr/TESSA.R +23 -30
- biopipen/utils/common_docstrs.py +3 -0
- biopipen/utils/mutate_helpers.R +110 -106
- {biopipen-0.22.0.dist-info → biopipen-0.22.2.dist-info}/METADATA +1 -1
- {biopipen-0.22.0.dist-info → biopipen-0.22.2.dist-info}/RECORD +27 -22
- {biopipen-0.22.0.dist-info → biopipen-0.22.2.dist-info}/entry_points.txt +1 -0
- {biopipen-0.22.0.dist-info → biopipen-0.22.2.dist-info}/WHEEL +0 -0
biopipen/utils/mutate_helpers.R
CHANGED
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@@ -1,6 +1,7 @@
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suppressPackageStartupMessages(library(rlang))
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suppressPackageStartupMessages(library(tidyselect))
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suppressPackageStartupMessages(library(dplyr))
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+
suppressPackageStartupMessages(library(tidyr))
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#' Get expanded, collapsed, emerged or vanished clones from a meta data frame
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#'
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@@ -15,6 +16,8 @@ suppressPackageStartupMessages(library(dplyr))
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#' be used as `ident_2`.
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#' @param subset An expression to subset the cells, will be passed to
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#' `dplyr::filter()`. Default is `TRUE` (no filtering).
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#' @param each A column name (without quotes) in metadata to split the cells.
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#' Each comparison will be done for each value in this column.
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#' @param id The column name (without quotes) in metadata for the
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#' group ids (i.e. `CDR3.aa`)
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#' @param compare Either a (numeric) column name (i.e. `Clones`, without quotes)
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#' @param uniq Whether to return unique ids or not. Default is `TRUE`.
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#' If `FALSE`, you can mutate the meta data frame with the returned ids.
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#' For example, `df %>% mutate(expanded = expanded(...))`.
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#' @param debug Return the transformed data frame with counts, predicates, sum, and diff.
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#' @param order The order of the returned ids. It could be `sum` or `diff`,
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#' which is the sum or diff of the `compare` between idents. Two kinds of
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#' modifiers can be added, including `desc` and `abs`. For example,
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id,
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compare,
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fun,
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each,
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uniq,
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order
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order,
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debug
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) {
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if (length(idents) == 1) {
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ident_1 <- idents[1]
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if (!compare_is_count && !compare_label %in% colnames(df)) {
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stop(paste0(
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"`compare` must be either a column name in df, or 'count'/'n'. ",
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"`compare` must be either a column name in df, or 'count'/'.n'. ",
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'Got "',
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compare_label,
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'"'
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))
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}
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predicate <- function(
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predicate <- function(ident_1, ident_2) {
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if (fun == "expanded") {
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ident_1 > ident_2 && ident_2 > 0
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} else if (fun == "expanded+") {
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ident_1 > ident_2
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} else if (fun == "collapsed") {
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ident_1 < ident_2 && ident_1 > 0
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} else if (fun == "collapsed+") {
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-
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ident_1 < ident_2
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} else if (fun == "emerged") {
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ident_1 > 0 && ident_2 == 0
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} else if (fun == "vanished") {
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ident_1 == 0 && ident_2 > 0
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}
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}
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# subset the data frame
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trans <- df %>%
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#
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trans <- df %>%
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dplyr::filter(!!subset) %>%
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drop_na(!!id) %>%
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# # remove NA values in group.by column
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# dplyr::filter(!is.na(!!group.by)) %>%
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# mark the group.by column (as .group) as ident_1 or ident_2 or NA
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mutate(
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.group = if_else(
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!!group.by == ident_1,
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"ident_1",
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if_else(ident_2 != "<NULL>" & !!group.by != ident_2, NA, "ident_2")
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)
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) %>%
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# remove NA values in ..group column
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d$..group == "ident_1", "ident_2", "ident_1"
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),
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..compare = 0
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)
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}
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d
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}) %>%
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# make sure ident_1 and ident_2 are in order
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arrange(..group, .by_group = TRUE) %>%
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drop_na(.group)
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if (is.null(each)) {
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trans <- trans %>% group_by(!!id, .group)
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} else {
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trans <- trans %>% group_by(!!each, !!id, .group)
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}
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if (compare_is_count) {
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trans <- trans %>% summarise(.n = n(), .groups = "drop")
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} else {
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trans <- trans %>% summarise(.n = first(!!compare), .groups = "drop")
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}
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trans <- trans %>% pivot_wider(names_from = .group, values_from = .n) %>%
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replace_na(list(ident_1 = 0, ident_2 = 0)) %>%
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rowwise() %>%
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# add the predicates, sums and diffs
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mutate(
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.predicate = predicate(ident_1, ident_2),
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.sum = ident_1 + ident_2,
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.diff = ident_1 - ident_2
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) %>%
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ungroup() %>%
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arrange(!!order)
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order_desc <- grepl("desc", order)
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order_abs <- grepl("abs", order)
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if (order_sum && !order_desc) {
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out <- trans %>% arrange(..sum) %>% pull(!!id)
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} else if (order_sum) {
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out <- trans %>% arrange(desc(..sum)) %>% pull(!!id)
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} else if (order_diff && !order_desc && !order_abs) {
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out <- trans %>% arrange(..diff) %>% pull(!!id)
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} else if (order_diff && !order_desc && order_abs) {
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out <- trans %>% arrange(abs(..diff)) %>% pull(!!id)
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} else if (order_diff && order_desc && !order_abs) {
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out <- trans %>% arrange(desc(..diff)) %>% pull(!!id)
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} else if (order_diff && order_desc && order_abs) {
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out <- trans %>% arrange(desc(abs(..diff))) %>% pull(!!id)
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} else {
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out <- trans %>% pull(!!id)
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if (debug) {
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return(trans)
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}
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uniq_ids <- trans %>% filter(.predicate) %>% pull(!!id) %>% as.vector() %>% unique()
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if (uniq) {
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return(uniq_ids)
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}
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out <- df %>% pull(!!id)
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out[!out %in% uniq_ids] <- NA
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out
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}
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#' @export
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group.by, # nolint
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subset = TRUE,
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each = NULL,
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id = CDR3.aa,
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compare =
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compare = .n,
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uniq = TRUE,
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debug = FALSE,
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order = desc(.sum),
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include_emerged = FALSE
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) {
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}
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fun = if (include_emerged) "expanded+" else "expanded"
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.size_compare(
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df,
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enquo(group.by),
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idents,
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enquo(id),
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enquo(compare),
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fun,
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df = df,
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group.by = enquo(group.by),
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idents = idents,
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subset = enquo(subset),
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id = enquo(id),
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compare = enquo(compare),
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fun = fun,
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each = tryCatch(enquo(each), error = function(e) NULL),
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uniq = uniq,
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order = order
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order = enexpr(order),
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debug = debug
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)
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}
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idents,
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subset = TRUE,
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each = NULL,
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id = CDR3.aa,
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compare =
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compare = .n,
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uniq = TRUE,
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debug = FALSE,
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order = desc(.sum),
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include_vanished = FALSE
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) {
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lbl <- as_label(enquo(df))
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fun = if (include_vanished) "collapsed+" else "collapsed"
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.size_compare(
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df,
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enquo(group.by),
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idents,
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enquo(subset),
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enquo(id),
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enquo(compare),
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fun,
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df = df,
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group.by = enquo(group.by),
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idents = idents,
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subset = enquo(subset),
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id = enquo(id),
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compare = enquo(compare),
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fun = fun,
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each = tryCatch(enquo(each), error = function(e) NULL),
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uniq = uniq,
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order = order
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order = enexpr(order),
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debug = debug
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)
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}
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subset = TRUE,
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each = NULL,
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id = CDR3.aa,
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compare =
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compare = .n,
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uniq = TRUE,
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debug = FALSE,
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order = desc(.sum)
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) {
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lbl <- as_label(enquo(df))
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if (length(lbl) == 1 && lbl == ".") {
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df <- across(everything())
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}
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.size_compare(
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df,
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enquo(group.by),
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idents,
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enquo(subset),
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enquo(id),
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enquo(compare),
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"emerged",
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df = df,
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group.by = enquo(group.by),
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idents = idents,
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subset = enquo(subset),
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id = enquo(id),
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compare = enquo(compare),
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fun = "emerged",
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each = tryCatch(enquo(each), error = function(e) NULL),
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uniq = uniq,
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order = order
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order = enexpr(order),
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debug = debug
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}
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@@ -309,25 +309,29 @@ vanished <- function(
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group.by, # nolint
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idents,
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subset = TRUE,
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each = NULL,
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id = CDR3.aa,
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compare =
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compare = .n,
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uniq = TRUE,
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-
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debug = FALSE,
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