AMS-BP 0.0.231__py3-none-any.whl → 0.0.251__py3-none-any.whl

AMS_BP/__init__.py

@@ -10,4 +10,4 @@ Last updated: 2024-12-16
 
 """
 
-__version__ = "0.0.231"
+__version__ = "0.0.251"

AMS_BP/optics/lasers/laser_profiles.py

@@ -96,7 +96,10 @@ class LaserParameters:
             Power in watts
         """
         if callable(self.power):
-            return self.power(t)
+            power = self.power(t)
+            if power < 0:
+                raise ValueError("Laser Power Cannot be Negative")
+            return power
         return self.power
 
     def get_position(self, t: float) -> Tuple[float, float, float]:
@@ -413,7 +416,7 @@ class WidefieldBeam(LaserProfile):
         base_intensity = power / (np.pi * self.max_radius**2)
 
         # Apply radial intensity profile with smooth falloff at edges
-        edge_width = self.max_radius * 0.1  # 10% of max radius
+        edge_width = self.max_radius * 0.00001 
         radial_profile = 0.5 * (1 - np.tanh((r - self.max_radius) / edge_width))
         # Apply DoF-based axial intensity profile
         axial_profile = self._calculate_dof_profile(z_shifted)
@@ -422,32 +425,6 @@ class WidefieldBeam(LaserProfile):
         return base_intensity * radial_profile * axial_profile
 
 
-# Example usage
-if __name__ == "__main__":
-    # Create parameters for a typical microscope objective
-    params = LaserParameters(
-        wavelength=488,  # 488 nm
-        power=0.001,  # 1 mW
-        beam_width=0.25,  # 250 nm
-        numerical_aperture=1.4,
-        refractive_index=1.518,  # Oil immersion
-    )
-
-    # Create beam object
-    beam = GaussianBeam(params)
-
-    # Get intensity map
-    result = beam.get_intensity_map(
-        volume_size=(5, 5, 10),  # 5x5x10 microns
-        voxel_size=0.1,  # 100 nm voxels
-        t=0,  # t=0 seconds
-    )
-
-    # print(f"Beam waist: {params.beam_width:.3f} µm")
-    # print(f"Rayleigh range: {params.rayleigh_range:.3f} µm")
-    # print(f"Diffraction limit: {params.diffraction_limited_width:.3f} µm")
-
-
 class HiLoBeam(LaserProfile):
     """
     Highly Inclined Laminated Optical (HiLo) illumination profile.
@@ -549,143 +526,3 @@ class HiLoBeam(LaserProfile):
         lamination_factor = np.exp(-np.abs(z_shifted) / (2 * self.axial_resolution))
 
         return intensity * lamination_factor
-
-
-class ConfocalBeam(LaserProfile):
-    """
-    Confocal microscopy beam profile with point scanning and pinhole characteristics.
-
-    Implements key optical principles of confocal microscopy:
-    - Point scanning illumination
-    - Pinhole-based rejection of out-of-focus light
-    - Depth-resolved imaging capabilities
-    """
-
-    def __init__(
-        self,
-        params: LaserParameters,
-        pinhole_diameter: float,  # Pinhole diameter in microns
-        scanning_mode: str = "point",  # 'point' or 'line'
-        line_orientation: str = "horizontal",  # 'horizontal' or 'vertical'
-    ):
-        """
-        Initialize Confocal beam profile.
-
-        Args:
-            params: LaserParameters for the beam
-            pinhole_diameter: Diameter of the detection pinhole in microns
-            scanning_mode: Scanning method ('point' or 'line')
-            line_orientation: Orientation for line scanning
-        """
-        super().__init__(params)
-
-        # Validate numerical aperture
-        if params.numerical_aperture is None:
-            raise ValueError(
-                "Numerical aperture must be specified for confocal microscopy"
-            )
-
-        # Pinhole and optical characteristics
-        self.pinhole_diameter = pinhole_diameter
-        self.scanning_mode = scanning_mode
-        self.line_orientation = line_orientation
-
-        # Calculate optical parameters
-        wavelength_microns = params.wavelength / 1000.0
-        na = params.numerical_aperture
-
-        # Theoretical resolution calculations
-        self.lateral_resolution = 0.61 * wavelength_microns / na
-        self.axial_resolution = 0.5 * wavelength_microns / (na**2)
-
-        # Pinhole transmission calculation
-        # Airy disk radius calculation
-        self.airy_radius = 1.22 * wavelength_microns / (2 * na)
-
-        # Transmission through pinhole
-        def pinhole_transmission(z):
-            """
-            Calculate pinhole transmission as a function of z-position.
-            Uses an error function to model smooth transition.
-            """
-            # Normalized z-position relative to focal plane
-            z_norm = z / self.axial_resolution
-
-            # Smooth transition function
-            return 0.5 * (1 + np.tanh(-z_norm))
-
-        self.pinhole_transmission = pinhole_transmission
-
-        # print("Confocal Microscopy Configuration:")
-        # print(f"  Scanning Mode: {scanning_mode}")
-        # print(f"  Pinhole Diameter: {pinhole_diameter:.2f} µm")
-        # print(f"  Lateral Resolution: {self.lateral_resolution:.3f} µm")
-        # print(f"  Axial Resolution: {self.axial_resolution:.3f} µm")
-        # print(f"  Airy Disk Radius: {self.airy_radius:.3f} µm")
-
-    def calculate_intensity(
-        self,
-        x: np.ndarray | float,
-        y: np.ndarray | float,
-        z: np.ndarray | float,
-        t: float,
-    ) -> np.ndarray:
-        """
-        Calculate the confocal illumination intensity distribution.
-
-        Args:
-            x: X coordinates in microns (3D array)
-            y: Y coordinates in microns (3D array)
-            z: Z coordinates in microns (3D array)
-            t: Time in seconds
-
-        Returns:
-            3D array of intensities in W/µm²
-        """
-        # Get time-dependent parameters
-        power = self.params.get_power(t)
-        pos = self.params.get_position(t)
-
-        # Shift coordinates based on current beam position
-        x_shifted = x - pos[0]
-        y_shifted = y - pos[1]
-        z_shifted = z - pos[2]
-
-        # Base beam parameters
-        w0 = self.params.beam_width  # Beam waist
-        zR = self.params.rayleigh_range  # Rayleigh range
-
-        # Calculate beam width at z
-        w_z = w0 * np.sqrt(1 + (z_shifted / zR) ** 2)
-
-        # Peak intensity calculation
-        I0 = 2 * power / (np.pi * w0**2)
-
-        # Scanning mode intensity modification
-        if self.scanning_mode == "point":
-            # Point scanning: standard Gaussian beam
-            radial_intensity = (
-                I0
-                * (w0 / w_z) ** 2
-                * np.exp(-2 * (x_shifted**2 + y_shifted**2) / w_z**2)
-            )
-        elif self.scanning_mode == "line":
-            # Line scanning: different intensity distribution
-            if self.line_orientation == "horizontal":
-                line_intensity = (
-                    I0 * (w0 / w_z) ** 2 * np.exp(-2 * y_shifted**2 / w_z**2)
-                )
-                radial_intensity = line_intensity
-            else:  # vertical line scanning
-                line_intensity = (
-                    I0 * (w0 / w_z) ** 2 * np.exp(-2 * x_shifted**2 / w_z**2)
-                )
-                radial_intensity = line_intensity
-        else:
-            raise ValueError(f"Unknown scanning mode: {self.scanning_mode}")
-
-        # Pinhole transmission effect
-        pinhole_effect = self.pinhole_transmission(z_shifted)
-
-        # Final intensity calculation
-        return radial_intensity * pinhole_effect

AMS_BP/photophysics/state_kinetics.py

@@ -27,6 +27,7 @@ class StateTransitionCalculator:
         self.current_global_time = current_global_time  # ms (oversample motion time)
         self.laser_intensity_generator = laser_intensity_generator
         self.fluorescent_state_history = {}  # {fluorescent.state.name : [delta time (seconds), laser_intensites], ...}
+        self.current_global_time_s = self.current_global_time * 1e-3
 
     def __call__(
         self,
@@ -48,7 +49,9 @@ class StateTransitionCalculator:
         time = 0
         transitions = self.flurophoreobj.state_history[self.current_global_time][2]
         final_state_name = transitions[0].from_state
-        laser_intensities = self._initialize_state_hist(self.current_global_time, time)
+        laser_intensities = self._initialize_state_hist(
+            self.current_global_time, time + self.current_global_time_s
+        )
 
         while time < self.time_duration:
             stateTransitionMatrixR = [

AMS_BP/sim_microscopy.py

@@ -73,7 +73,9 @@ class VirtualMicroscope:
     def _set_laser_powers(self, laser_power: Dict[str, float]) -> None:
         if laser_power is not None:
             for laser in laser_power.keys():
-                if isinstance(self.lasers[laser].params.power, float):
+                if isinstance(self.lasers[laser].params.power, float) and isinstance(
+                    laser_power[laser], float
+                ):
                     if laser_power[laser] > self.lasers[laser].params.max_power:
                         raise ValueError(
                             "Provided laser power for laser: {} nm, is larger than the maximum power: {}".format(

{ams_bp-0.0.231.dist-info → ams_bp-0.0.251.dist-info}/METADATA

@@ -1,6 +1,6 @@
 Metadata-Version: 2.4
 Name: AMS_BP
-Version: 0.0.231
+Version: 0.0.251
 Summary: Advanced Microscopy Simulations developed for the Weber Lab by Baljyot Singh Parmar
 Project-URL: Documentation, https://joemans3.github.io/AMS_BP/
 Project-URL: Source code, https://github.com/joemans3/AMS_BP
@@ -171,3 +171,5 @@ frames, metadata = function_exp(microscope=microscope, config=config_exp)
 from AMS_BP.configio.saving import save_config_frames
 save_config_frames(metadata, frames, setup_config["base_config"].OutputParameters)
 ```
+
+> A more detailed example is provided in the jupyter notebook in the examples. For starters refer to the [VisualizingIndividualModules](examples/VisualizingIndividualModules/modules_explained.ipynb). Then head over to the [laser modulation module](examples/VisualizingIndividualModules/laser_modulation.ipynb) which will show how to change the laser power over time in the simulations. Then view an example of a complex experiment setup for [FRAP](examples/QuantitativeExperiments/FRAP_methods.ipynb) which is possible by the use of compositions of modules in this simulation library.

{ams_bp-0.0.231.dist-info → ams_bp-0.0.251.dist-info}/RECORD

@@ -1,7 +1,7 @@
-AMS_BP/__init__.py,sha256=rqdkAwhLvzoA4UNC7EgJOgze5vTlp72Cm62WfiIINCc,328
+AMS_BP/__init__.py,sha256=qv5C9LGYuQzZP9NQ_ST5KH4IuqGwi8PVl44IypUo97w,328
 AMS_BP/run_cell_simulation.py,sha256=7InopFikjo0HfaLO2siXskBIbyCIte9avG4YXjjaWCI,7420
 AMS_BP/sim_config.toml,sha256=3IqOQIJYmP5g4okk15nqQiNZb3ij7Pt63HbpI-5tySw,11672
-AMS_BP/sim_microscopy.py,sha256=0UZfyT44nrB4JdfnFnRPTVBm3tPbCyOnPXiBBZs8xIc,18617
+AMS_BP/sim_microscopy.py,sha256=u60ApTA6MTUmqSAd7EsAxweKya_Typput8NumDq9fp8,18697
 AMS_BP/cells/__init__.py,sha256=yWFScBC1uOGDkeC8i1m1ZBtIREcyt4JHxYa72LxbBZU,177
 AMS_BP/cells/base_cell.py,sha256=FIPB9J8F40tb53vv7C6qG-SaAFLOI8-MGIk1mmZ-gnI,1503
 AMS_BP/cells/rectangular_cell.py,sha256=5yGxvTXYvgldLXyWXpE_SD9Zx2NLerC-I2j02reHsJ0,2515
@@ -29,12 +29,12 @@ AMS_BP/optics/filters/filters.py,sha256=-iw7eqmDO77SEqlFTv5jJNVwpA8y93TLsjy5hhsA
 AMS_BP/optics/filters/channels/__init__.py,sha256=47DEQpj8HBSa-_TImW-5JCeuQeRkm5NMpJWZG3hSuFU,0
 AMS_BP/optics/filters/channels/channelschema.py,sha256=SConyA5yVdfnI_8sgcxVC8SV7S8tGUJYPPC6jn7lglU,906
 AMS_BP/optics/lasers/__init__.py,sha256=T7dHohhyLf_pBw4TidarYHWmiwxVXGE71-Bf1aeBbuc,564
-AMS_BP/optics/lasers/laser_profiles.py,sha256=J9czY646XcW8GzXx9Eb16mG7tQdWw4oVYveOrihZCeY,22745
+AMS_BP/optics/lasers/laser_profiles.py,sha256=DM3YDh6JccgQk8fjBRh6J3bDpTChm8Im_AE5z9EK3EI,17101
 AMS_BP/optics/psf/__init__.py,sha256=ezrKPgpTeR4gTHOvF0mhF6u2zMMTd8Bgp8PGeOf11fA,121
 AMS_BP/optics/psf/psf_engine.py,sha256=Do54D1jMbSrj5uljdTrrEttCvxq3qbVT74acRuOk15c,9434
 AMS_BP/photophysics/__init__.py,sha256=47DEQpj8HBSa-_TImW-5JCeuQeRkm5NMpJWZG3hSuFU,0
 AMS_BP/photophysics/photon_physics.py,sha256=QRG_QIZ4csJ3g5qGP9Wtk7kzqm8_MUbVHfFef6cMtHQ,6671
-AMS_BP/photophysics/state_kinetics.py,sha256=0cc7Vc4LtAbEdGDeg22IJmRGLsONOty4c32hXHO-TSU,5281
+AMS_BP/photophysics/state_kinetics.py,sha256=IdZtlHCLs--iSjLwDu2IQA617qXC4la8VpqosrM-vgQ,5401
 AMS_BP/probabilityfuncs/__init__.py,sha256=47DEQpj8HBSa-_TImW-5JCeuQeRkm5NMpJWZG3hSuFU,0
 AMS_BP/probabilityfuncs/markov_chain.py,sha256=LV6KGr8Lv4NIvBPJqsR0CEynssa_mPH30qLaK85GObA,4339
 AMS_BP/probabilityfuncs/probability_functions.py,sha256=j_rIxrupGBf_FKkQBh1TvEa34A44jAasaZQRg2u3FuY,11793
@@ -48,8 +48,8 @@ AMS_BP/utils/decorators.py,sha256=4qFdvzPJne0dhkhD1znPxRln1Rfr5NX8rdcCDcbATRU,62
 AMS_BP/utils/errors.py,sha256=7BOd-L4_YeKmWn3Q4EOdTnNF3Bj_exDa3eg5X0yCZrc,759
 AMS_BP/utils/maskMaker.py,sha256=2ca3n2nc8rFtUh1LurKXOJJsUmhrOpWbRnVX7fjRVvs,335
 AMS_BP/utils/util_functions.py,sha256=jI6WBh09_khdABnEoVK7SK1WRvCLHuw40f5ALyflzlc,9478
-ams_bp-0.0.231.dist-info/METADATA,sha256=Rs9gQc6T1-ZcguRTLWfsl42ZsTmJCtVgXRn-pcL7noY,5284
-ams_bp-0.0.231.dist-info/WHEEL,sha256=qtCwoSJWgHk21S1Kb4ihdzI2rlJ1ZKaIurTj_ngOhyQ,87
-ams_bp-0.0.231.dist-info/entry_points.txt,sha256=MFUK9bZWW61djfsavqopMqiVPVn4lJtt6v8qzyEFyNM,76
-ams_bp-0.0.231.dist-info/licenses/LICENSE,sha256=k_-JV1DQKvO0FR8WjvOisqdTl0kp6VJ7RFM3YZhao0c,1071
-ams_bp-0.0.231.dist-info/RECORD,,
+ams_bp-0.0.251.dist-info/METADATA,sha256=lSmQVE_Aaw13hf692nC4MuTZplqmg5vBqjap8PTnow0,5870
+ams_bp-0.0.251.dist-info/WHEEL,sha256=qtCwoSJWgHk21S1Kb4ihdzI2rlJ1ZKaIurTj_ngOhyQ,87
+ams_bp-0.0.251.dist-info/entry_points.txt,sha256=MFUK9bZWW61djfsavqopMqiVPVn4lJtt6v8qzyEFyNM,76
+ams_bp-0.0.251.dist-info/licenses/LICENSE,sha256=k_-JV1DQKvO0FR8WjvOisqdTl0kp6VJ7RFM3YZhao0c,1071
+ams_bp-0.0.251.dist-info/RECORD,,