@teselagen/sequence-utils 0.1.22 → 0.1.24

package/src/addGapsToSeqReads.test.js

@@ -0,0 +1,358 @@
+import addGapsToSeqReads from "./addGapsToSeqReads.js";
+
+describe("cigar strings to gapped alignments", () => {
+  it("adds gaps into sequencing reads before starting bp pos and from own deletions & other seq reads' insertions", () => {
+    const refSeq = { name: "ref seq", sequence: "GGGAGACACC" };
+    const seqReads = [
+      { name: "r1", seq: "GATTGAC", pos: 3, cigar: "2M2I3M", reversed: false },
+      { name: "r2", seq: "GAGAGAC", pos: 3, cigar: "7M", reversed: false },
+      {
+        name: "r3",
+        seq: "GGGAGATCAC",
+        pos: 1,
+        cigar: "6M1I3M",
+        reversed: false
+      },
+      { name: "r4", seq: "GATTGAC", pos: 3, cigar: "2M2I3M", reversed: false },
+      { name: "r5", seq: "GAGC", pos: 3, cigar: "3M1D1M", reversed: false },
+      {
+        name: "r6",
+        seq: "GAGCTTACC",
+        pos: 3,
+        cigar: "3M1D1M2I3M",
+        reversed: true
+      },
+      {
+        name: "r7",
+        seq: "GGCATTTCC",
+        pos: 2,
+        cigar: "2M3D2M3I2M",
+        reversed: true
+      },
+      {
+        name: "r8",
+        seq: "GGATTGACATT",
+        pos: 1,
+        cigar: "1D3M2I4M2I2D",
+        reversed: true
+      },
+      {
+        name: "r9",
+        seq: "GGTTTGACCTTT",
+        pos: 1,
+        cigar: "2M3I2D1M2D3M3I",
+        reversed: true
+      }
+    ];
+    const result = addGapsToSeqReads(refSeq, seqReads);
+    expect(result).toEqual([
+      // ref seq first
+      { name: "ref seq", sequence: "GG---GA--GA-C--A---CC---" },
+      // then seq reads
+      {
+        name: "r1",
+        sequence: "-----GATTGA-C-----------",
+        cigar: "2M2I3M",
+        reversed: false
+      },
+      {
+        name: "r2",
+        sequence: "-----GA--GA-G--A---C----",
+        cigar: "7M",
+        reversed: false
+      },
+      {
+        name: "r3",
+        sequence: "GG---GA--GATC--A---C----",
+        cigar: "6M1I3M",
+        reversed: false
+      },
+      {
+        name: "r4",
+        sequence: "-----GATTGA-C-----------",
+        cigar: "2M2I3M",
+        reversed: false
+      },
+      {
+        name: "r5",
+        sequence: "-----GA--G--C-----------",
+        cigar: "3M1D1M",
+        reversed: false
+      },
+      {
+        name: "r6",
+        sequence: "-----GA--G--CTTA---CC---",
+        cigar: "3M1D1M2I3M",
+        reversed: true
+      },
+      {
+        name: "r7",
+        sequence: "-G---G------C--ATTTCC---",
+        cigar: "2M3D2M3I2M",
+        reversed: true
+      },
+      {
+        name: "r8",
+        sequence: "-G---GATTGA-C--A-TT-----",
+        cigar: "1D3M2I4M2I2D",
+        reversed: true
+      },
+      {
+        name: "r9",
+        sequence: "GGTTT----G-----A---CCTTT",
+        cigar: "2M3I2D1M2D3M3I",
+        reversed: true
+      }
+    ]);
+  });
+
+  it("removes unaligned seq reads (seqRead.pos = 0, seqRead.cigar = null)", () => {
+    const refSeq = { name: "ref seq", sequence: "GGACCGGAACAGGAAGCAAGGGACAG" };
+    const seqReads = [
+      {
+        name: "r1",
+        seq: "GAAGCAAGGGACSSSSS",
+        pos: 13,
+        cigar: "12M5S",
+        reversed: false
+      },
+      { name: "r2", seq: "ZZZZZ", pos: 0, cigar: null, reversed: false }
+    ];
+    const result = addGapsToSeqReads(refSeq, seqReads);
+    expect(result).toEqual([
+      // ref seq first
+      { name: "ref seq", sequence: "GGACCGGAACAGGAAGCAAGGGACAG---" },
+      // then seq reads
+      {
+        name: "r1",
+        sequence: "------------GAAGCAAGGGACSSSSS",
+        cigar: "12M5S",
+        reversed: false
+      }
+    ]);
+  });
+
+  it("adjusts bp pos of alignment with the ref seq (seqRead.pos) if there are soft-clipped reads at the beginning of a seq read (#S at start of seqRead.cigar)...seq read aligned near the beginning of the ref seq", () => {
+    const refSeq = { name: "ref seq", sequence: "GGGAGACACC" };
+    const seqReads = [
+      {
+        name: "r1",
+        seq: "SSGATTGAC",
+        pos: 3,
+        cigar: "2S2M2I3M",
+        reversed: false
+      }
+    ];
+    const result = addGapsToSeqReads(refSeq, seqReads);
+    expect(result).toEqual([
+      // ref seq first
+      { name: "ref seq", sequence: "GGGA--GACACC" },
+      // then seq reads
+      {
+        name: "r1",
+        sequence: "SSGATTGAC---",
+        cigar: "2S2M2I3M",
+        reversed: false
+      }
+    ]);
+  });
+
+  it("adjusts bp pos of alignment with the ref seq (seqRead.pos) if there are soft-clipped reads at the beginning of a seq read (#S at start of seqRead.cigar)...seq read aligned near the middle of the ref seq", () => {
+    const refSeq = { name: "ref seq", sequence: "GGACCGGAACAGGAAGCAAGGGACAG" };
+    const seqReads = [
+      {
+        name: "r1",
+        seq: "SSSGAAGCAAG",
+        pos: 13,
+        cigar: "3S8M",
+        reversed: false
+      }
+    ];
+    const result = addGapsToSeqReads(refSeq, seqReads);
+    expect(result).toEqual([
+      // ref seq first
+      { name: "ref seq", sequence: "GGACCGGAACAGGAAGCAAGGGACAG" },
+      // then seq reads
+      {
+        name: "r1",
+        sequence: "---------SSSGAAGCAAG------",
+        cigar: "3S8M",
+        reversed: false
+      }
+    ]);
+  });
+
+  it("adjusts bp pos of alignment with the ref seq (seqRead.pos) if there are soft-clipped reads at the beginning of a seq read (#S at start of seqRead.cigar)...multiple seq reads with #S at the start", () => {
+    const refSeq = { name: "ref seq", sequence: "GGACCGGAACAGGAAGCAAGGGACAG" };
+    const seqReads = [
+      {
+        name: "r1",
+        seq: "SSACTTCGGAACAGGAAG",
+        pos: 3,
+        cigar: "2S2M2I12M",
+        reversed: false
+      },
+      {
+        name: "r2",
+        seq: "SSSGAAGCAAG",
+        pos: 13,
+        cigar: "3S8M",
+        reversed: false
+      }
+    ];
+    const result = addGapsToSeqReads(refSeq, seqReads);
+    expect(result).toEqual([
+      // ref seq first
+      { name: "ref seq", sequence: "GGAC--CGGAACAGGAAGCAAGGGACAG" },
+      // then seq reads
+      {
+        name: "r1",
+        sequence: "SSACTTCGGAACAGGAAG----------",
+        cigar: "2S2M2I12M",
+        reversed: false
+      },
+      {
+        name: "r2",
+        sequence: "-----------SSSGAAGCAAG------",
+        cigar: "3S8M",
+        reversed: false
+      }
+    ]);
+  });
+
+  it("adjusts bp pos of alignment with the ref seq (seqRead.pos) if there are soft-clipped reads at the beginning of a seq read (#S at start of seqRead.cigar)...soft-clipped reads before the beginning of the ref seq", () => {
+    const refSeq = { name: "ref seq", sequence: "GGGAGACACC" };
+    const seqReads = [
+      {
+        name: "r1",
+        seq: "SSSGGGATTGAC",
+        pos: 1,
+        cigar: "3S4M2I3M",
+        reversed: false
+      }
+    ];
+    const result = addGapsToSeqReads(refSeq, seqReads);
+    expect(result).toEqual([
+      // ref seq first
+      { name: "ref seq", sequence: "---GGGA--GACACC" },
+      // then seq reads
+      {
+        name: "r1",
+        sequence: "SSSGGGATTGAC---",
+        cigar: "3S4M2I3M",
+        reversed: false
+      }
+    ]);
+  });
+
+  it("works with soft-clipped reads at the end of a seq read (#S at end of seqRead.cigar)", () => {
+    const refSeq = { name: "ref seq", sequence: "GGACCGGAACAGGAAGCAAGGGACAG" };
+    const seqReads = [
+      {
+        name: "r1",
+        seq: "GAAGCAAGSSS",
+        pos: 13,
+        cigar: "12M5S",
+        reversed: false
+      }
+    ];
+    const result = addGapsToSeqReads(refSeq, seqReads);
+    expect(result).toEqual([
+      // ref seq first
+      { name: "ref seq", sequence: "GGACCGGAACAGGAAGCAAGGGACAG" },
+      // then seq reads
+      {
+        name: "r1",
+        sequence: "------------GAAGCAAGSSS---",
+        cigar: "12M5S",
+        reversed: false
+      }
+    ]);
+  });
+
+  it("accounts for soft-clipped reads at the end of a seq read (#S at end of seqRead.cigar) that make seqRead.sequence longer than refSeq.sequence, by making ref seq & seq reads all the same/longest length", () => {
+    const refSeq = { name: "ref seq", sequence: "GGACCGGAACAGGAAGCAAGGGACAG" };
+    const seqReads = [
+      {
+        name: "r1",
+        seq: "GAAGCAAGGGACSSSSS",
+        pos: 13,
+        cigar: "12M5S",
+        reversed: false
+      },
+      { name: "r2", seq: "GCAAG", pos: 16, cigar: "5M", reversed: false }
+    ];
+    const result = addGapsToSeqReads(refSeq, seqReads);
+    expect(result).toEqual([
+      // ref seq first
+      { name: "ref seq", sequence: "GGACCGGAACAGGAAGCAAGGGACAG---" },
+      // then seq reads
+      {
+        name: "r1",
+        sequence: "------------GAAGCAAGGGACSSSSS",
+        cigar: "12M5S",
+        reversed: false
+      },
+      {
+        name: "r2",
+        sequence: "---------------GCAAG---------",
+        cigar: "5M",
+        reversed: false
+      }
+    ]);
+  });
+
+  it("adjusts bp pos of alignment with the ref seq (seqRead.pos) if there are soft-clipped reads at the beginning of a seq read (#S at start of seqRead.cigar)...soft-clipped reads before the beginning of the ref seq", () => {
+    const refSeq = { name: "ref seq", sequence: "GGGAGACACC" };
+    const seqReads = [
+      {
+        name: "r1",
+        seq: "SSSGGGATTGAC",
+        pos: 1,
+        cigar: "3S4M2I3M",
+        reversed: false
+      },
+      { name: "r2", seq: "GGAGAC", pos: 2, cigar: "6M", reversed: false },
+      {
+        name: "r3",
+        seq: "SSGGGATTGAC",
+        pos: 1,
+        cigar: "2S4M2I3M",
+        reversed: false
+      },
+      { name: "r4", seq: "SSCAC", pos: 7, cigar: "2S3M", reversed: false },
+      { name: "r5", seq: "SSSSSCAC", pos: 7, cigar: "5S3M", reversed: false }
+    ];
+    const result = addGapsToSeqReads(refSeq, seqReads);
+    expect(result).toEqual([
+      // ref seq first
+      { name: "ref seq", sequence: "---GGGA--GACACC" },
+      // then seq reads
+      {
+        name: "r1",
+        sequence: "SSSGGGATTGAC---",
+        cigar: "3S4M2I3M",
+        reversed: false
+      },
+      { name: "r2", sequence: "----GGA--GAC---", cigar: "6M", reversed: false },
+      {
+        name: "r3",
+        sequence: "-SSGGGATTGAC---",
+        cigar: "2S4M2I3M",
+        reversed: false
+      },
+      {
+        name: "r4",
+        sequence: "---------SSCAC-",
+        cigar: "2S3M",
+        reversed: false
+      },
+      {
+        name: "r5",
+        sequence: "----SSS--SSCAC-",
+        cigar: "5S3M",
+        reversed: false
+      }
+    ]);
+  });
+});

package/src/adjustAnnotationsToInsert.js

@@ -0,0 +1,19 @@
+import {adjustRangeToInsert} from "@teselagen/range-utils";
+import {map} from "lodash";
+
+export default function adjustAnnotationsToInsert(
+  annotationsToBeAdjusted,
+  insertStart,
+  insertLength
+) {
+  return map(annotationsToBeAdjusted, annotation => {
+    return {
+      ...adjustRangeToInsert(annotation, insertStart, insertLength),
+      ...(annotation.locations && {
+        locations: annotation.locations.map(loc =>
+          adjustRangeToInsert(loc, insertStart, insertLength)
+        )
+      })
+    };
+  });
+};

package/src/adjustBpsToReplaceOrInsert.js

@@ -0,0 +1,50 @@
+import {
+  splitRangeIntoTwoPartsIfItIsCircular,
+  getSequenceWithinRange,
+  getRangeLength,
+  invertRange,
+  isPositionWithinRange,
+} from "@teselagen/range-utils";
+
+import spliceString from "string-splice";
+
+export default function adjustBpsToReplaceOrInsert(
+  bpString,
+  insertString = "",
+  caretPositionOrRange,
+) {
+  let stringToReturn = bpString;
+
+  if (caretPositionOrRange && caretPositionOrRange.start > -1) {
+    if (
+      getRangeLength(caretPositionOrRange, bpString.length) === bpString.length
+    ) {
+      return insertString;
+    }
+    const ranges = splitRangeIntoTwoPartsIfItIsCircular(
+      invertRange(caretPositionOrRange, bpString.length)
+    );
+    stringToReturn = "";
+    ranges.forEach((range, index) => {
+      stringToReturn += getSequenceWithinRange(range, bpString);
+      if (ranges.length === 1) {
+        if (isPositionWithinRange(0, range, bpString.length, true, true)) {
+          stringToReturn = stringToReturn + insertString;
+        } else {
+          stringToReturn = insertString + stringToReturn;
+        }
+      } else {
+        if (index === 0) stringToReturn += insertString;
+      }
+    });
+  } else {
+    //caretPosition Passed
+    stringToReturn = spliceString(
+      bpString,
+      caretPositionOrRange,
+      0,
+      insertString
+    );
+  }
+  return stringToReturn;
+};

package/src/adjustBpsToReplaceOrInsert.test.js

@@ -0,0 +1,59 @@
+//tnr: half finished test.
+
+
+import chai from "chai";
+import chaiSubset from "chai-subset";
+
+import adjustBpsToReplaceOrInsert from "./adjustBpsToReplaceOrInsert";
+
+chai.should();
+chai.use(chaiSubset);
+
+describe("adjustBpsToReplaceOrInsert", () => {
+  it("inserts characters at correct caret position", () => {
+    adjustBpsToReplaceOrInsert("tttgggaaaccc", "xxx", 0).should.equal(
+      "xxxtttgggaaaccc"
+    );
+  });
+  it("inserts characters at correct caret position", () => {
+    adjustBpsToReplaceOrInsert("tttgggaaaccc", "xxx", 3).should.equal(
+      "tttxxxgggaaaccc"
+    );
+  });
+
+  it("inserts characters at correct caret position", () => {
+    adjustBpsToReplaceOrInsert("tttgggaaaccc", "xxx", 12).should.equal(
+      "tttgggaaacccxxx"
+    );
+  });
+  it("can replace whole sequence with upper case", () => {
+    adjustBpsToReplaceOrInsert("tttgggaaaccc", "TTTGGGAAACCC", {
+      start: 0,
+      end: 11
+    }).should.equal("TTTGGGAAACCC");
+  });
+  it("can replace whole sequence with just a couple chars", () => {
+    adjustBpsToReplaceOrInsert("tttgggaaaccc", "xx", {
+      start: 0,
+      end: 11
+    }).should.equal("xx");
+  });
+  it("inserts characters at correct range 0 0", () => {
+    adjustBpsToReplaceOrInsert("tttgggaaaccc", "xxx", {
+      start: 0,
+      end: 0
+    }).should.equal("xxxttgggaaaccc");
+  });
+  it("inserts characters at correct range 11 11", () => {
+    adjustBpsToReplaceOrInsert("tttgggaaaccc", "xxx", {
+      start: 11,
+      end: 11
+    }).should.equal("tttgggaaaccxxx");
+  });
+  it("inserts characters at correct range 11 0", () => {
+    adjustBpsToReplaceOrInsert("tttgggaaaccc", "xxx", {
+      start: 11,
+      end: 0
+    }).should.equal("xxxttgggaaacc");
+  });
+});