@sjcrh/proteinpaint-server 2.98.2-1 → 2.99.0
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- package/package.json +4 -4
- package/routes/correlationVolcano.js +3 -9
- package/routes/termdb.DE.js +7 -18
- package/routes/termdb.singlecellSamples.js +22 -56
- package/src/app.js +47 -102
- package/utils/corr.R +4 -2
- package/utils/edge.R +13 -13
package/utils/corr.R
CHANGED
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@@ -19,7 +19,9 @@ coeffs <- c()
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pvalues <- c()
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sample_sizes <- c()
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for (i in 1:length(v1)) {
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-
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+
suppressWarnings({
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cor <- cor.test(as.numeric(unlist(v1[i])), as.numeric(unlist(v2[i])), method = input$method)
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})
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coeffs <- c(coeffs, cor$estimate)
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pvalues <- c(pvalues, cor$p.value)
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sample_sizes <- c(sample_sizes, length(as.numeric(unlist(v1[i]))))
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@@ -33,4 +35,4 @@ names(output)[2] <- "correlation"
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names(output)[3] <- "original_p_value"
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names(output)[4] <- "adjusted_p_value"
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names(output)[5] <- "sample_size"
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-
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+
toJSON(output)
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package/utils/edge.R
CHANGED
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@@ -19,7 +19,7 @@ read_json_time <- system.time({
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controls <- unlist(strsplit(input$control, ","))
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combined <- c("geneID", "geneSymbol", cases, controls)
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})
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cat("Time to read JSON: ", read_json_time[3], " seconds\n")
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#cat("Time to read JSON: ", read_json_time[3], " seconds\n")
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# Read counts data
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read_counts_time <- system.time({
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@@ -59,7 +59,7 @@ read_counts_time <- system.time({
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stop("Unknown storage type")
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}
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})
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cat("Time to read counts data: ", read_counts_time[3], " seconds\n")
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#cat("Time to read counts data: ", read_counts_time[3], " seconds\n")
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# Create conditions vector
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conditions <- c(rep("Diseased", length(cases)), rep("Control", length(controls)))
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@@ -69,19 +69,19 @@ gene_id_symbols <- paste0(geneIDs, "\t", geneSymbols)
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dge_list_time <- system.time({
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y <- DGEList(counts = read_counts, group = conditions, genes = gene_id_symbols)
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})
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cat("Time to generate DGEList: ", dge_list_time[3], " seconds\n")
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#cat("Time to generate DGEList: ", dge_list_time[3], " seconds\n")
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# Filter and normalize counts
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filter_time <- system.time({
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keep <- filterByExpr(y, min.count = input$min_count, min.total.count = input$min_total_count)
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})
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cat("Time to filter by expression: ", filter_time[3], " seconds\n")
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#cat("Time to filter by expression: ", filter_time[3], " seconds\n")
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normalization_time <- system.time({
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y <- y[keep, keep.lib.sizes = FALSE]
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y <- calcNormFactors(y, method = "TMM")
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})
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cat("Normalization time: ", normalization_time[3], " seconds\n")
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#cat("Normalization time: ", normalization_time[3], " seconds\n")
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# Differential expression analysis
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if (length(input$conf1) == 0) { # No adjustment of confounding factors
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@@ -92,33 +92,33 @@ if (length(input$conf1) == 0) { # No adjustment of confounding factors
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})
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})
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})
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cat("Dispersion time: ", dispersion_time[3], " seconds\n")
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#cat("Dispersion time: ", dispersion_time[3], " seconds\n")
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exact_test_time <- system.time({
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et <- exactTest(y)
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})
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cat("Exact test time: ", exact_test_time[3], " seconds\n")
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#cat("Exact test time: ", exact_test_time[3], " seconds\n")
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} else { # Adjusting for confounding factors
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y$samples <- data.frame(conditions = conditions, conf1 = input$conf1)
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model_gen_time <- system.time({
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design <- model.matrix(~ conf1 + conditions, data = y$samples)
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})
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cat("Time for making design matrix: ", model_gen_time[3], " seconds\n")
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#cat("Time for making design matrix: ", model_gen_time[3], " seconds\n")
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dispersion_time <- system.time({
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y <- estimateDisp(y, design)
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})
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cat("Dispersion time: ", dispersion_time[3], " seconds\n")
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#cat("Dispersion time: ", dispersion_time[3], " seconds\n")
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fit_time <- system.time({
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fit <- glmFit(y, design)
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})
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cat("Fit time: ", fit_time[3], " seconds\n")
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#cat("Fit time: ", fit_time[3], " seconds\n")
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test_statistics_time <- system.time({
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et <- glmLRT(fit, coef = 2)
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})
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cat("Test statistics time: ", test_statistics_time[3], " seconds\n")
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#cat("Test statistics time: ", test_statistics_time[3], " seconds\n")
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}
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# Multiple testing correction
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@@ -137,7 +137,7 @@ multiple_testing_correction_time <- system.time({
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names(output)[4] <- "original_p_value"
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names(output)[5] <- "adjusted_p_value"
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})
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cat("Time for multiple testing correction: ", multiple_testing_correction_time[3], " seconds\n")
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#cat("Time for multiple testing correction: ", multiple_testing_correction_time[3], " seconds\n")
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# Output results
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-
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toJSON(output)
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