@jbrowse/cli 4.3.0 → 5.0.0-beta.10
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- package/README.md +451 -72
- package/dist/base.js +0 -3
- package/dist/bin.js +2 -1
- package/dist/cliFetch.js +3 -0
- package/dist/commands/add-assembly/index.js +46 -19
- package/dist/commands/add-assembly/utils.js +204 -290
- package/dist/commands/add-connection.js +73 -79
- package/dist/commands/add-track-json.js +24 -12
- package/dist/commands/add-track-utils/adapter-utils.js +189 -288
- package/dist/commands/add-track-utils/file-operations.js +27 -11
- package/dist/commands/add-track-utils/multiwig.js +47 -0
- package/dist/commands/add-track-utils/track-config.js +66 -25
- package/dist/commands/add-track-utils/validators.js +36 -20
- package/dist/commands/add-track.js +200 -62
- package/dist/commands/admin-server/index.js +39 -50
- package/dist/commands/admin-server/utils.js +238 -126
- package/dist/commands/create.js +18 -34
- package/dist/commands/make-density/density-generator.js +180 -0
- package/dist/commands/make-density/index.js +139 -0
- package/dist/commands/make-pif/index.js +133 -11
- package/dist/commands/make-pif/pif-generator.js +243 -43
- package/dist/commands/process-utils.js +13 -2
- package/dist/commands/remove-track.js +20 -13
- package/dist/commands/set-default-session.js +35 -31
- package/dist/commands/shared/config-operations.js +3 -10
- package/dist/commands/shared/density.js +12 -0
- package/dist/commands/shared/sort-utils.js +67 -10
- package/dist/commands/shared/validators.js +38 -3
- package/dist/commands/sort-bed.js +2 -33
- package/dist/commands/sort-gff.js +2 -33
- package/dist/commands/text-index/adapter-utils.js +19 -26
- package/dist/commands/text-index/aggregate.js +40 -34
- package/dist/commands/text-index/command.js +37 -7
- package/dist/commands/text-index/config-utils.js +62 -85
- package/dist/commands/text-index/file-list.js +32 -18
- package/dist/commands/text-index/index.js +4 -4
- package/dist/commands/text-index/indexing-utils.js +44 -96
- package/dist/commands/text-index/per-track.js +44 -31
- package/dist/commands/text-index/validators.js +1 -1
- package/dist/commands/upgrade.js +20 -32
- package/dist/commands/validate/configManifest.generated.js +7613 -0
- package/dist/commands/validate/configSchema.generated.js +20400 -0
- package/dist/commands/validate/displayDefaultKeys.js +28 -0
- package/dist/commands/validate/index.js +113 -0
- package/dist/commands/validate/liftConfig.js +34 -0
- package/dist/commands/validate/markRules/aggregateFieldName.js +9 -0
- package/dist/commands/validate/markRules/autoBin.js +27 -0
- package/dist/commands/validate/markRules/colorScale.js +244 -0
- package/dist/commands/validate/markRules/cssColorsLevel4.js +162 -0
- package/dist/commands/validate/markRules/jexlStrings.js +23 -0
- package/dist/commands/validate/markRules/markProblems.js +532 -0
- package/dist/commands/validate/markRules/markRuleFacts.js +6 -0
- package/dist/commands/validate/markRules/markSpecs.js +96 -0
- package/dist/commands/validate/markRules/markVocabulary.js +57 -0
- package/dist/commands/validate/markRules/shapeNameList.js +7 -0
- package/dist/commands/validate/markRules/stepChannels.js +55 -0
- package/dist/commands/validate/schemaValidate.js +425 -0
- package/dist/commands/validate/suggest.js +35 -0
- package/dist/commands/validate/types.js +6 -0
- package/dist/commands/validate/validateConfig.js +502 -0
- package/dist/index.js +104 -50
- package/dist/types/common.js +3 -27
- package/dist/utils.js +309 -128
- package/dist/version.js +1 -1
- package/package.json +8 -14
- package/bin/run +0 -4
- package/bundle/index.js +0 -3686
- package/dist/commands/make-pif/cigar-utils.js +0 -24
- package/dist/commands/make-pif/file-utils.js +0 -30
- package/dist/commands/track-utils.js +0 -74
- package/dist/util.js +0 -35
package/README.md
CHANGED
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@@ -13,7 +13,7 @@ or by using a script file.
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A simple script that does not use @jbrowse/cli at all may just look like this
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```
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```js
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const config = JSON.parse(fs.readFileSync('config.json', 'utf8'))
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// do something with config.tracks, config.assemblies, etc.
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fs.writeFileSync('config.json', JSON.stringify(config, null, 2))
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It is likely preferable in most cases to install the tools globally with
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`npm install @jbrowse/cli -g` however
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```
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```text
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JBrowse CLI
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@@ -54,12 +54,14 @@ COMMANDS
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create Downloads and installs the latest JBrowse 2 release
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add-assembly Add an assembly to a JBrowse 2 configuration
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add-track Add a track to a JBrowse 2 configuration
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validate Check a configuration for errors, including ones JBrowse accepts silently
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text-index Make a text-indexing file for any given track(s)
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admin-server Start up a small admin server for JBrowse configuration
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upgrade Upgrades JBrowse 2 to latest version
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make-pif Creates pairwise indexed PAF (PIF), with bgzip and tabix
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sort-
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make-density Counts feature starts per bin into a bigWig density sidecar, with bedGraphToBigWig
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sort-gff Sort a GFF/GTF for tabix: sort -k1,1 -k4,4n, header kept on top
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sort-bed Sort a BED for tabix: sort -k1,1 -k2,2n, header kept on top
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add-connection Add a connection to a JBrowse 2 configuration
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add-track-json Add a track configuration directly from a JSON hunk
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remove-track Remove a track configuration from a JBrowse 2 configuration
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## jbrowse create
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```
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```text
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Downloads and installs the latest JBrowse 2 release
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Usage: jbrowse create [localPath] [options]
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-t, --tag Version of JBrowse 2 to install. Format is v1.0.0.
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Defaults to latest
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Examples:
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# Download latest release from github, and put in specific path
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$ jbrowse create /path/to/new/installation
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## jbrowse add-assembly
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```
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```text
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Add an assembly to a JBrowse 2 configuration
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Usage: jbrowse add-assembly <sequence> [options]
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Options:
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-t, --type type of sequence, by default inferred from sequence
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file
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indexedFasta - An index FASTA (e.g. .fa or .fasta)
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file; can optionally specify --faiLocation
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bgzipFasta - A block-gzipped and indexed FASTA
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(e.g. .fa.gz or .fasta.gz) file; can optionally
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specify --faiLocation and/or --gziLocation
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twoBit - A twoBit (e.g. .2bit) file
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chromSizes - A chromosome sizes (e.g. .chrom.sizes)
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file
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custom - Either a JSON file location or inline JSON
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that defines a custom sequence adapter; must
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provide --name if using inline JSON [choices:
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any file operations [choices: copy, symlink, move,
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inPlace]
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-f, --force Overwrite existing assembly and skip file existence
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checks
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--config Any extra config settings to add to the assembly
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name exists
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Notes:
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--config takes inline JSON (not a file path) that is merged into the generated
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assembly config, so you can set fields the dedicated flags do not cover. A
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"sequence" object in it is merged into the generated ReferenceSequenceTrack
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rather than replacing it, e.g. --config
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'{"sequence":{"formatAbout":{"hideUris":true}}}' hides file URIs in the
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assembly's About dialog.
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Examples:
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# add assembly to installation in current directory. assumes .fai file also exists, and copies GRCh38.fa and GRCh38.fa.fai to current directory
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$ jbrowse add-assembly GRCh38.fa --load copy
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# add a bgzip indexed fasta inferred by fa.gz extension. assumes .fa.gz.gzi and .fa.gz.fai files also exists
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$ jbrowse add-assembly myfile.fa.gz --load copy
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# hide file URIs in the assembly About dialog
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$ jbrowse add-assembly GRCh38.fa --load copy --config '{"sequence":{"formatAbout":{"hideUris":true}}}'
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```
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## jbrowse add-track
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```
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```text
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Add a track to a JBrowse 2 configuration
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Usage: jbrowse add-track <track> [options]
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Options:
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-h, --help
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-h, --help Show help
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-t, --trackType Type of track, by default inferred from track file
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--adapterType Adapter type, by default inferred from track file
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-n, --name Name of the track. Will be defaulted to the trackId
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if none specified
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-d, --description Optional description of the track
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-a, --assemblyNames Assembly name or names for track as comma separated
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string
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string. For pairwise synteny tracks the order is
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query,target (reverse of minimap2/nucmer argument
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order); for the multi-genome PAF adapters
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(MultiGenomePAFAdapter/MultiGenomeIndexedPAFAdapter)
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list every assembly the file covers, in any order
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--category Optional comma separated string of categories to
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group tracks
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--config Any extra config settings to add to a track
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--color Track color: a plain CSS color or a jexl callback.
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--height Track display height in pixels. Merged into
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displayDefaults
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--displayDefaults Inline JSON merged into the track displayDefaults
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(labels, mouseover, filter, etc.)
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--multiwig Build a MultiQuantitativeTrack from several BigWigs
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(in place of the positional track arg): a
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subadapter objects, each with its own name/color
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--target Path to config file in JB2 installation to write
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--skipCheck Skip check for whether file or URL exists
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--overwrite Overwrites existing track if it shares the same
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trackId
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-l, --load How to manage the track file relative to
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config.json. Required for local files, omit for
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URLs [choices: copy, symlink, move, inPlace]
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-f, --force Overwrite existing track and any existing files
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--protocol Force protocol to a specific value
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--bed2 Used only for mcscan anchors/simpleAnchors types
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--density Path or URL of a features-per-bin bigWig (jbrowse
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make-density) to attach as the adapter's
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densityAdapter. A local <file>.density.bw beside
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the track file is attached without this flag
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Notes:
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--load controls how the data file is placed relative to config.json: copy, move,
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or symlink it into the install directory, or inPlace to reference a pre-staged
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local file where it already sits. Omit --load entirely for URLs. For a local
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file the matching index is whichever conventional spelling is actually beside it
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— <file>.bai/.crai/.tbi, a <file>.csi, or the reads.bai form Picard writes
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beside reads.bam. A URL cannot be checked, so it gets <file>.bai/.tbi; pass
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--indexFile for anything else.
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--config takes inline JSON (not a file path) that is merged into the generated
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track config, so you can set fields the dedicated flags do not cover, e.g.
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--config '{"metadata":{"skipTextIndex":true}}' to exclude the track from jbrowse
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text-index.
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--color and --height set the two most common appearance settings without writing
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JSON. Wrap the value in single quotes and use double quotes inside a jexl
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callback so nothing needs escaping, e.g. --color
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'jexl:feature.strand==1?"blue":"red"'. --displayDefaults takes inline JSON for
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any other appearance setting (labels, mouseover, filter).
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--multiwig bundles several BigWigs into one MultiQuantitativeTrack, in place of
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the positional track argument: pass a comma-separated list of BigWig files/URLs,
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or a .json file with an array of BigWig locations or subadapter objects (each
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carrying its own name/color/group). With --load, local list entries are copied
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like any other track file.
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--density attaches a features-per-bin bigWig (jbrowse make-density) as the
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adapter's densityAdapter, the band a display draws where the region is too large
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to fetch features. A local <file>.density.bw beside the track file is attached
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without the flag; a URL cannot be probed, so a remote sidecar needs it. The
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adapters carrying the slot are BamAdapter, CramAdapter, HtsgetBamAdapter,
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Gff3TabixAdapter, GtfTabixAdapter, BedTabixAdapter, BigBedAdapter,
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VcfTabixAdapter and SplitVcfTabixAdapter.
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For pairwise synteny adapters (PAF/Delta/Chain) --assemblyNames is query,target
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— the reverse of the minimap2/nucmer input order. For the multi-genome PAF
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adapters (MultiGenomePAFAdapter, MultiGenomeIndexedPAFAdapter) it is instead the
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full list of assemblies the file covers, in any order.
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Examples:
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# copy /path/to/my.bam and /path/to/my.bam.bai to current directory and adds track to config.json
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$ jbrowse add-track /path/to/my.bam --load copy
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# --load inPlace adds a track without doing file operations
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$ jbrowse add-track /url/relative/path.bam --load inPlace
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# color a track by strand and set its height (no escaping: single-quote the value, double-quote inside the jexl)
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$ jbrowse add-track genes.gff3.gz --load copy --color 'jexl:feature.strand==1?"blue":"red"' --height 200
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# bundle several BigWigs into one MultiQuantitativeTrack (no positional track arg)
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$ jbrowse add-track --multiwig a.bw,b.bw,c.bw --load copy --name "Coverage"
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# ...or from a sources.json carrying per-row name/color for each BigWig
|
|
386
|
+
$ jbrowse add-track --multiwig sources.json --name "CATlas ATAC"
|
|
387
|
+
|
|
388
|
+
# attach a remote density sidecar (a local genes.gff3.density.bw needs no flag)
|
|
389
|
+
$ jbrowse add-track https://mywebsite.com/genes.gff3.gz --density https://mywebsite.com/genes.gff3.density.bw
|
|
299
390
|
```
|
|
300
391
|
|
|
301
392
|
|
|
302
|
-
## jbrowse
|
|
393
|
+
## jbrowse validate
|
|
394
|
+
|
|
395
|
+
```text
|
|
396
|
+
Check a JBrowse configuration for errors, including the ones JBrowse itself
|
|
397
|
+
accepts silently
|
|
398
|
+
|
|
399
|
+
Usage: jbrowse validate [config.json] [options]
|
|
400
|
+
|
|
401
|
+
Options:
|
|
402
|
+
--json Output the findings as JSON instead of text
|
|
403
|
+
|
|
404
|
+
-q, --quiet Only print errors, suppressing warnings and notes
|
|
405
|
+
|
|
406
|
+
-h, --help Show help
|
|
407
|
+
|
|
408
|
+
Notes:
|
|
409
|
+
|
|
410
|
+
A config key JBrowse does not recognize is ignored rather than reported, so a
|
|
411
|
+
misspelled slot leaves the track loading normally with the setting doing
|
|
412
|
+
nothing. That is what this command is mainly for.
|
|
413
|
+
|
|
414
|
+
Two levels are reported:
|
|
415
|
+
|
|
416
|
+
error JBrowse silently does the wrong thing, or refuses to load it — an
|
|
417
|
+
unknown slot, a key a defaultSession view or display does not
|
|
418
|
+
declare, a track naming an assembly the config never defines, a
|
|
419
|
+
defaultSession naming a trackId that does not exist, a duplicate
|
|
420
|
+
trackId. Exits 1.
|
|
421
|
+
|
|
422
|
+
warning JBrowse will complain by itself on load, or handles it — a type name
|
|
423
|
+
the core plugins do not register (expected if one of your plugins
|
|
424
|
+
registers it), or a legacy key a migration rewrites, such as a view
|
|
425
|
+
nesting its settings under "init". Never fails the run.
|
|
426
|
+
|
|
427
|
+
A LinearMarkDisplay loads whenever its keys and value types are right and says
|
|
428
|
+
what it cannot draw in a corner notice rather than refusing the track. This
|
|
429
|
+
command runs the display's own rule list over every "marks" list in the file: an
|
|
430
|
+
error where a mark draws nothing, never draws or a step cannot run, and a
|
|
431
|
+
warning where a slot waits unread or the marks draw in an arrangement the author
|
|
432
|
+
may not have meant. With --json each finding carries the stable "rule" id of the
|
|
433
|
+
rule that made it.
|
|
434
|
+
|
|
435
|
+
Types registered by plugins are not known to this command, so they come through
|
|
436
|
+
as warnings rather than errors.
|
|
437
|
+
|
|
438
|
+
The same checks are published as a JSON Schema at
|
|
439
|
+
https://jbrowse.org/jb2/schema/v5/config.json: a "$schema" line naming it at the
|
|
440
|
+
top of a config.json gives an editor completion and validation for every slot.
|
|
441
|
+
This command runs that schema first, then the cross-references a schema cannot
|
|
442
|
+
express — a trackId a session names that no track defines, an assembly a track
|
|
443
|
+
names that none defines.
|
|
303
444
|
|
|
445
|
+
Examples:
|
|
446
|
+
|
|
447
|
+
# check the config.json in the current directory
|
|
448
|
+
$ jbrowse validate
|
|
449
|
+
|
|
450
|
+
# check a specific config or saved session
|
|
451
|
+
$ jbrowse validate /path/to/config.json
|
|
452
|
+
$ jbrowse validate mysession.jbrowse
|
|
453
|
+
|
|
454
|
+
# machine-readable output
|
|
455
|
+
$ jbrowse validate config.json --json
|
|
304
456
|
```
|
|
457
|
+
|
|
458
|
+
|
|
459
|
+
## jbrowse text-index
|
|
460
|
+
|
|
461
|
+
```text
|
|
305
462
|
Make a text-indexing file for any given track(s).
|
|
306
463
|
|
|
307
464
|
Usage: jbrowse text-index [options]
|
|
@@ -314,7 +471,9 @@ Options:
|
|
|
314
471
|
available tracks
|
|
315
472
|
|
|
316
473
|
--excludeTracks Specific tracks to exclude from indexing, formatted
|
|
317
|
-
as comma separated trackIds
|
|
474
|
+
as comma separated trackIds. To exclude a track
|
|
475
|
+
permanently, set metadata.skipTextIndex on it in
|
|
476
|
+
config.json instead (see Notes)
|
|
318
477
|
|
|
319
478
|
--target Path to config file in JB2 installation directory
|
|
320
479
|
to read from.
|
|
@@ -322,7 +481,7 @@ Options:
|
|
|
322
481
|
--out Synonym for target
|
|
323
482
|
|
|
324
483
|
--attributes Comma separated list of attributes to index
|
|
325
|
-
[default: Name,ID]
|
|
484
|
+
[default: Name,ID,symbol]
|
|
326
485
|
|
|
327
486
|
-a, --assemblies Specify the assembl(ies) to create an index for. If
|
|
328
487
|
unspecified, creates an index for each assembly in
|
|
@@ -335,8 +494,13 @@ Options:
|
|
|
335
494
|
|
|
336
495
|
--perTrack If set, creates an index per track [default: false]
|
|
337
496
|
|
|
338
|
-
--exclude
|
|
339
|
-
CDS,exon]
|
|
497
|
+
--exclude Comma separated list of feature types to exclude
|
|
498
|
+
from indexing [default: CDS,exon]
|
|
499
|
+
|
|
500
|
+
--include Comma separated list of feature types to index,
|
|
501
|
+
dropping every other type. Unset by default, which
|
|
502
|
+
indexes every type --exclude does not name. GFF3
|
|
503
|
+
only
|
|
340
504
|
|
|
341
505
|
--prefixSize Specify the prefix size for the ixx index. We
|
|
342
506
|
attempt to automatically calculate this, but you
|
|
@@ -355,6 +519,34 @@ Options:
|
|
|
355
519
|
--dryrun Just print out tracks that will be indexed by the
|
|
356
520
|
process, without doing any indexing
|
|
357
521
|
|
|
522
|
+
Notes:
|
|
523
|
+
|
|
524
|
+
Individual tracks in config.json can be permanently excluded from indexing by
|
|
525
|
+
setting "metadata": { "skipTextIndex": true } on the track. Such tracks are
|
|
526
|
+
skipped even when indexing all tracks or a whole assembly, so you do not have to
|
|
527
|
+
pass --excludeTracks on every run.
|
|
528
|
+
|
|
529
|
+
Only tracks with an indexable adapter type (Gff3Adapter, Gff3TabixAdapter,
|
|
530
|
+
GtfAdapter, GtfTabixAdapter, VcfAdapter, VcfTabixAdapter) are indexed; tracks
|
|
531
|
+
with other adapter types are skipped automatically.
|
|
532
|
+
|
|
533
|
+
GTF has no Name/ID attributes, so the default --attributes also match their GTF
|
|
534
|
+
spellings (gene_name, transcript_name, gene_id, transcript_id). A GFF3 row with
|
|
535
|
+
no Name, as in GENCODE, is named by its own gene_name or transcript_name.
|
|
536
|
+
|
|
537
|
+
--exclude names types not to index; --include names the only types to index.
|
|
538
|
+
Reach for --include when the file draws from a vocabulary you do not control: an
|
|
539
|
+
NCBI RefSeq GFF3 uses 115 feature types, 80 of them leaf records with no name to
|
|
540
|
+
search (a match is labelled with a bare UUID, a cDNA_match with an MD5, every
|
|
541
|
+
biological_region with the string "biological region"), so a deny list leaks
|
|
542
|
+
whichever type is added next while the allow list — gene, pseudogene and the
|
|
543
|
+
transcript types — does not grow. Both may be given: --include admits, --exclude
|
|
544
|
+
then narrows. Either can also be set per track in config.json as
|
|
545
|
+
textSearching.indexingFeatureTypesToInclude / indexingFeatureTypesToExclude,
|
|
546
|
+
which takes precedence over the flag.
|
|
547
|
+
|
|
548
|
+
Examples:
|
|
549
|
+
|
|
358
550
|
# indexes all tracks that it can find in the current directory's config.json
|
|
359
551
|
$ jbrowse text-index
|
|
360
552
|
|
|
@@ -362,7 +554,7 @@ $ jbrowse text-index
|
|
|
362
554
|
$ jbrowse text-index --tracks=track1,track2,track3
|
|
363
555
|
|
|
364
556
|
# indexes all tracks except specific trackIds
|
|
365
|
-
$ jbrowse text-index --
|
|
557
|
+
$ jbrowse text-index --excludeTracks=track1,track2,track3
|
|
366
558
|
|
|
367
559
|
# indexes all tracks in a directory's config.json or in a specific config file
|
|
368
560
|
$ jbrowse text-index --out /path/to/jb2/
|
|
@@ -377,29 +569,54 @@ $ jbrowse text-index --file myfile.gff3.gz --file myfile.vcfgz --out indexes
|
|
|
377
569
|
|
|
378
570
|
## jbrowse admin-server
|
|
379
571
|
|
|
380
|
-
```
|
|
572
|
+
```text
|
|
381
573
|
Start up a small admin server for JBrowse configuration
|
|
382
574
|
|
|
383
575
|
Usage: jbrowse admin-server [options]
|
|
384
576
|
|
|
385
577
|
Options:
|
|
386
|
-
-h, --help
|
|
578
|
+
-h, --help Show help
|
|
387
579
|
|
|
388
580
|
-p, --port Specified port to start the server on (default:
|
|
389
581
|
9090)
|
|
390
582
|
|
|
583
|
+
--host Interface to bind to (default: 127.0.0.1). Use
|
|
584
|
+
0.0.0.0 to accept connections from other machines
|
|
585
|
+
|
|
391
586
|
--root Path to the root of the JB2 installation
|
|
392
587
|
|
|
393
588
|
--bodySizeLimit Size limit of the update message (default: 25mb)
|
|
394
589
|
|
|
590
|
+
Notes:
|
|
591
|
+
|
|
592
|
+
The admin-server lets a browser session write changes back to config.json on
|
|
593
|
+
disk, authorized by a one-time key printed in the startup URL. It binds to
|
|
594
|
+
127.0.0.1 so only this machine can reach it. The key gates the config routes,
|
|
595
|
+
but the rest of the JBrowse directory is served unauthenticated, so widen --host
|
|
596
|
+
only on a trusted network.
|
|
597
|
+
|
|
598
|
+
Examples:
|
|
599
|
+
|
|
600
|
+
# start the admin server for the JBrowse install in the current directory
|
|
395
601
|
$ jbrowse admin-server
|
|
602
|
+
|
|
603
|
+
# start on a specific port
|
|
396
604
|
$ jbrowse admin-server -p 8888
|
|
605
|
+
|
|
606
|
+
# point at a specific JBrowse installation directory
|
|
607
|
+
$ jbrowse admin-server --root /path/to/jb2/
|
|
608
|
+
|
|
609
|
+
# raise the body size limit for very large config updates
|
|
610
|
+
$ jbrowse admin-server --bodySizeLimit 100mb
|
|
611
|
+
|
|
612
|
+
# accept connections from other machines on the network
|
|
613
|
+
$ jbrowse admin-server --host 0.0.0.0
|
|
397
614
|
```
|
|
398
615
|
|
|
399
616
|
|
|
400
617
|
## jbrowse upgrade
|
|
401
618
|
|
|
402
|
-
```
|
|
619
|
+
```text
|
|
403
620
|
Upgrades JBrowse 2 to latest version
|
|
404
621
|
|
|
405
622
|
Usage: jbrowse upgrade [localPath] [options]
|
|
@@ -423,6 +640,8 @@ Options:
|
|
|
423
640
|
|
|
424
641
|
-u, --url A direct URL to a JBrowse 2 release
|
|
425
642
|
|
|
643
|
+
Examples:
|
|
644
|
+
|
|
426
645
|
# Upgrades current directory to latest jbrowse release
|
|
427
646
|
$ jbrowse upgrade
|
|
428
647
|
|
|
@@ -445,34 +664,135 @@ $ jbrowse upgrade --nightly
|
|
|
445
664
|
|
|
446
665
|
## jbrowse make-pif
|
|
447
666
|
|
|
448
|
-
```
|
|
667
|
+
```text
|
|
449
668
|
creates pairwise indexed PAF (PIF), with bgzip and tabix
|
|
450
669
|
|
|
451
670
|
Usage: jbrowse make-pif <file> [options]
|
|
452
671
|
|
|
453
672
|
Options:
|
|
454
|
-
-h, --help
|
|
673
|
+
-h, --help Show help
|
|
455
674
|
|
|
456
675
|
--out Where to write the output file. will write
|
|
457
676
|
${file}.pif.gz and ${file}.pif.gz.tbi
|
|
458
677
|
|
|
459
678
|
--csi Create a CSI index for the PIF file instead of TBI
|
|
460
679
|
|
|
461
|
-
|
|
680
|
+
--coarse Accuracy bound (bp) of the coarse tier. A coarse
|
|
681
|
+
row replaces its CIGAR with a coarse CIGAR (cr:Z:)
|
|
682
|
+
that keeps every indel longer than half this length
|
|
683
|
+
and folds the rest into runs, each run staying
|
|
684
|
+
within this many bp of the real alignment. Defaults
|
|
685
|
+
to 10000. The coarse tier (prefix T/Q) is emitted
|
|
686
|
+
alongside the per-row CIGAR fine tier by default so
|
|
687
|
+
whole-genome synteny views can auto-switch to it;
|
|
688
|
+
pass --no-coarse to omit it.
|
|
689
|
+
|
|
690
|
+
--no-coarse Do not emit the coarse tier; write only the per-row
|
|
691
|
+
CIGAR fine tier.
|
|
692
|
+
|
|
693
|
+
--threads Compression threads for bgzip. Defaults to 4. Raise
|
|
694
|
+
it on a machine with cores to spare, or set 1 to
|
|
695
|
+
leave the rest of the machine alone.
|
|
696
|
+
|
|
697
|
+
Notes:
|
|
698
|
+
|
|
699
|
+
Use --csi for assemblies containing sequences longer than ~512 Mb. The default
|
|
700
|
+
TBI index cannot address coordinates beyond 2^29 (~536 Mb), so a CSI index is
|
|
701
|
+
required for large chromosomes (e.g. some plant and amphibian genomes). Requires
|
|
702
|
+
sh, sort, bgzip, and tabix on the PATH.
|
|
703
|
+
|
|
704
|
+
Examples:
|
|
705
|
+
|
|
706
|
+
# creates input.pif.gz and input.pif.gz.tbi in the same directory
|
|
707
|
+
$ jbrowse make-pif input.paf
|
|
462
708
|
|
|
463
|
-
|
|
709
|
+
# specify the output file, also creates output.pif.gz.tbi
|
|
710
|
+
$ jbrowse make-pif input.paf --out output.pif.gz
|
|
711
|
+
|
|
712
|
+
# use a CSI index for assemblies with chromosomes longer than ~512 Mb
|
|
713
|
+
$ jbrowse make-pif input.paf --csi
|
|
714
|
+
|
|
715
|
+
# a looser coarse tier: runs within 50kb of the alignment, indels over 25kb kept
|
|
716
|
+
$ jbrowse make-pif input.paf --coarse 50000
|
|
717
|
+
|
|
718
|
+
# emit only the per-row CIGAR fine tier, skipping the coarse tier
|
|
719
|
+
$ jbrowse make-pif input.paf --no-coarse
|
|
720
|
+
|
|
721
|
+
# give bgzip more (or fewer) compression threads than the default 4
|
|
722
|
+
$ jbrowse make-pif input.paf --threads 8
|
|
464
723
|
```
|
|
465
724
|
|
|
466
725
|
|
|
467
|
-
## jbrowse
|
|
726
|
+
## jbrowse make-density
|
|
727
|
+
|
|
728
|
+
```text
|
|
729
|
+
Counts feature starts per bin into a bigWig, the density sidecar a track draws
|
|
730
|
+
where the region is too large to fetch its features
|
|
731
|
+
|
|
732
|
+
Usage: jbrowse make-density <file> [options]
|
|
733
|
+
|
|
734
|
+
Options:
|
|
735
|
+
-h, --help Show help
|
|
736
|
+
|
|
737
|
+
--bin Width of each bin, in bp [default: 1000]
|
|
738
|
+
|
|
739
|
+
--chrom-sizes Two-column reference name and length table for the
|
|
740
|
+
assembly the file is on
|
|
741
|
+
|
|
742
|
+
--assembly FASTA of the assembly the file is on; its .fai
|
|
743
|
+
supplies the reference lengths, in place of
|
|
744
|
+
--chrom-sizes
|
|
745
|
+
|
|
746
|
+
--out Where to write the bigWig. Defaults to
|
|
747
|
+
<file>.density.bw beside the input, which is where
|
|
748
|
+
add-track looks for it
|
|
749
|
+
|
|
750
|
+
Notes:
|
|
751
|
+
|
|
752
|
+
The sidecar is attached to a track through its adapter's densityAdapter slot,
|
|
753
|
+
which BamAdapter, CramAdapter, HtsgetBamAdapter, Gff3TabixAdapter,
|
|
754
|
+
GtfTabixAdapter, BedTabixAdapter, BigBedAdapter, VcfTabixAdapter and
|
|
755
|
+
SplitVcfTabixAdapter declare. add-track attaches it on its own when the default
|
|
756
|
+
<file>.density.bw sits beside the track file, so building it before adding the
|
|
757
|
+
track is all it takes; --density attaches one by path or URL.
|
|
758
|
+
|
|
759
|
+
GFF3 counts only top-level features — a line whose attributes carry no Parent= —
|
|
760
|
+
so a gene is one count rather than one per exon. GTF, BED and VCF have no parent
|
|
761
|
+
link to follow, so every record counts.
|
|
468
762
|
|
|
763
|
+
BAM and CRAM are out of scope: read depth is not feature density, and the tools
|
|
764
|
+
that compute it already write bigWig. Point add-track --density at what they
|
|
765
|
+
produce.
|
|
766
|
+
|
|
767
|
+
Requires bedGraphToBigWig (UCSC) on the PATH.
|
|
768
|
+
|
|
769
|
+
Examples:
|
|
770
|
+
|
|
771
|
+
# writes genes.gff3.density.bw beside the input, in 1kb bins
|
|
772
|
+
$ jbrowse make-density genes.gff3.gz --chrom-sizes hg38.chrom.sizes
|
|
773
|
+
|
|
774
|
+
# take the reference lengths from the assembly FASTA index instead
|
|
775
|
+
$ jbrowse make-density variants.vcf.gz --assembly hg38.fa
|
|
776
|
+
|
|
777
|
+
# coarser bins, and a name of your own
|
|
778
|
+
$ jbrowse make-density genes.gff3.gz --assembly hg38.fa --bin 10000 --out genes.10kb.bw
|
|
469
779
|
```
|
|
470
|
-
|
|
780
|
+
|
|
781
|
+
|
|
782
|
+
## jbrowse sort-gff
|
|
783
|
+
|
|
784
|
+
```text
|
|
785
|
+
Sort a GFF or GTF for tabix — the two share a refname/start column layout. It is
|
|
786
|
+
`sort -k1,1 -k4,4n` with LC_ALL=C and a tab separator, plus every line starting
|
|
787
|
+
with # kept at the top rather than sorted into the data. Takes a file, or the
|
|
788
|
+
same data on stdin
|
|
471
789
|
|
|
472
790
|
Usage: jbrowse sort-gff [file] [options]
|
|
473
791
|
|
|
474
792
|
Options:
|
|
475
|
-
-h, --help
|
|
793
|
+
-h, --help Show help
|
|
794
|
+
|
|
795
|
+
Examples:
|
|
476
796
|
|
|
477
797
|
# sort gff and pipe to bgzip
|
|
478
798
|
$ jbrowse sort-gff input.gff | bgzip > sorted.gff.gz
|
|
@@ -480,18 +800,26 @@ $ tabix sorted.gff.gz
|
|
|
480
800
|
|
|
481
801
|
# sort gff from stdin
|
|
482
802
|
$ cat input.gff | jbrowse sort-gff | bgzip > sorted.gff.gz
|
|
803
|
+
|
|
804
|
+
# also works on GTF
|
|
805
|
+
$ jbrowse sort-gff input.gtf | bgzip > sorted.gtf.gz
|
|
806
|
+
$ tabix -p gff sorted.gtf.gz
|
|
483
807
|
```
|
|
484
808
|
|
|
485
809
|
|
|
486
810
|
## jbrowse sort-bed
|
|
487
811
|
|
|
488
|
-
```
|
|
489
|
-
|
|
812
|
+
```text
|
|
813
|
+
Sort a BED file for tabix. It is `sort -k1,1 -k2,2n` with LC_ALL=C and a tab
|
|
814
|
+
separator, plus every line starting with # kept at the top rather than sorted
|
|
815
|
+
into the data. Takes a file, or the same data on stdin
|
|
490
816
|
|
|
491
817
|
Usage: jbrowse sort-bed [file] [options]
|
|
492
818
|
|
|
493
819
|
Options:
|
|
494
|
-
-h, --help
|
|
820
|
+
-h, --help Show help
|
|
821
|
+
|
|
822
|
+
Examples:
|
|
495
823
|
|
|
496
824
|
# sort bed and pipe to bgzip
|
|
497
825
|
$ jbrowse sort-bed input.bed | bgzip > sorted.bed.gz
|
|
@@ -503,13 +831,13 @@ $ tabix sorted.bed.gz
|
|
|
503
831
|
|
|
504
832
|
## jbrowse add-connection
|
|
505
833
|
|
|
506
|
-
```
|
|
834
|
+
```text
|
|
507
835
|
Add a connection to a JBrowse 2 configuration
|
|
508
836
|
|
|
509
837
|
Usage: jbrowse add-connection <connectionUrlOrPath> [options]
|
|
510
838
|
|
|
511
839
|
Options:
|
|
512
|
-
-h, --help
|
|
840
|
+
-h, --help Show help
|
|
513
841
|
|
|
514
842
|
-t, --type Type of connection (e.g. JBrowse1Connection,
|
|
515
843
|
UCSCTrackHubConnection, custom)
|
|
@@ -532,31 +860,41 @@ Options:
|
|
|
532
860
|
|
|
533
861
|
--out Synonym for target
|
|
534
862
|
|
|
535
|
-
|
|
863
|
+
-f, --force Overwrite existing connection if one with the same
|
|
864
|
+
id exists
|
|
536
865
|
|
|
537
|
-
|
|
538
|
-
connection id
|
|
866
|
+
Examples:
|
|
539
867
|
|
|
540
|
-
|
|
868
|
+
# add a JBrowse 1 data directory connection (type inferred from the jbrowse/data path)
|
|
869
|
+
$ jbrowse add-connection https://mysite.com/jbrowse/data/ -a hg19
|
|
541
870
|
|
|
542
|
-
|
|
543
|
-
$ jbrowse add-connection
|
|
544
|
-
|
|
545
|
-
|
|
546
|
-
$ jbrowse add-connection
|
|
871
|
+
# force the JBrowse1Connection type for a non-standard data folder path
|
|
872
|
+
$ jbrowse add-connection https://mysite.com/jbrowse/custom_data_folder/ --type JBrowse1Connection -a hg38
|
|
873
|
+
|
|
874
|
+
# add a UCSC track hub (type inferred from the hub.txt filename)
|
|
875
|
+
$ jbrowse add-connection https://mysite.com/path/to/hub.txt
|
|
876
|
+
|
|
877
|
+
# force the UCSCTrackHubConnection type for a hub file not named hub.txt
|
|
878
|
+
$ jbrowse add-connection https://mysite.com/path/to/custom_hub_name.txt --type UCSCTrackHubConnection
|
|
879
|
+
|
|
880
|
+
# add a custom connection type with extra config
|
|
881
|
+
$ jbrowse add-connection https://mysite.com/path/to/custom --type custom --config '{"uri":{"url":"https://mysite.com/path/to/custom"}, "locationType": "UriLocation"}' -a hg19
|
|
882
|
+
|
|
883
|
+
# set an explicit id/name and write to a specific config file
|
|
547
884
|
$ jbrowse add-connection https://mysite.com/path/to/hub.txt --connectionId newId --name newName --target /path/to/jb2/installation/config.json
|
|
548
885
|
```
|
|
549
886
|
|
|
550
887
|
|
|
551
888
|
## jbrowse add-track-json
|
|
552
889
|
|
|
553
|
-
```
|
|
554
|
-
Add a track configuration directly from a JSON hunk to the JBrowse 2
|
|
890
|
+
```text
|
|
891
|
+
Add a track configuration directly from a JSON hunk to the JBrowse 2
|
|
892
|
+
configuration
|
|
555
893
|
|
|
556
894
|
Usage: jbrowse add-track-json <track> [options]
|
|
557
895
|
|
|
558
896
|
Options:
|
|
559
|
-
-h, --help
|
|
897
|
+
-h, --help Show help
|
|
560
898
|
|
|
561
899
|
-u, --update Update the contents of an existing track, matched
|
|
562
900
|
based on trackId
|
|
@@ -566,44 +904,74 @@ Options:
|
|
|
566
904
|
|
|
567
905
|
--out Synonym for target
|
|
568
906
|
|
|
907
|
+
Notes:
|
|
908
|
+
|
|
909
|
+
The track argument is a JSON file, inline JSON, or "-" to read the JSON from
|
|
910
|
+
stdin, so a track config can be generated and piped straight in.
|
|
911
|
+
|
|
912
|
+
Examples:
|
|
913
|
+
|
|
914
|
+
# add a track from a JSON file
|
|
569
915
|
$ jbrowse add-track-json track.json
|
|
916
|
+
|
|
917
|
+
# update an existing track (matched by trackId) with new JSON contents
|
|
570
918
|
$ jbrowse add-track-json track.json --update
|
|
919
|
+
|
|
920
|
+
# pass the track config inline instead of via a file
|
|
921
|
+
$ jbrowse add-track-json '{"type":"FeatureTrack","trackId":"genes","assemblyNames":["hg38"],"adapter":{"type":"Gff3TabixAdapter","gffGzLocation":{"uri":"genes.gff.gz"}}}'
|
|
922
|
+
|
|
923
|
+
# pipe the track config in from another program
|
|
924
|
+
$ jq '.tracks[0]' other/config.json | jbrowse add-track-json -
|
|
925
|
+
|
|
926
|
+
# write to a config.json in a specific installation directory
|
|
927
|
+
$ jbrowse add-track-json track.json --out /path/to/jb2/
|
|
571
928
|
```
|
|
572
929
|
|
|
573
930
|
|
|
574
931
|
## jbrowse remove-track
|
|
575
932
|
|
|
576
|
-
```
|
|
577
|
-
Remove a track configuration from a JBrowse 2 configuration. Be aware that this
|
|
933
|
+
```text
|
|
934
|
+
Remove a track configuration from a JBrowse 2 configuration. Be aware that this
|
|
935
|
+
can cause crashes in saved sessions that refer to this track!
|
|
578
936
|
|
|
579
937
|
Usage: jbrowse remove-track <trackId> [options]
|
|
580
938
|
|
|
581
939
|
Options:
|
|
582
|
-
-h, --help
|
|
940
|
+
-h, --help Show help
|
|
583
941
|
|
|
584
942
|
--target Path to config file in JB2 installation directory
|
|
585
943
|
to write out to
|
|
586
944
|
|
|
587
945
|
--out Synonym for target
|
|
588
946
|
|
|
589
|
-
|
|
947
|
+
Examples:
|
|
948
|
+
|
|
949
|
+
# remove a track from the config.json in the current directory
|
|
950
|
+
$ jbrowse remove-track my_track_id
|
|
951
|
+
|
|
952
|
+
# remove a track from a config.json in a specific installation directory
|
|
953
|
+
$ jbrowse remove-track my_track_id --out /path/to/jb2/
|
|
954
|
+
|
|
955
|
+
# remove a track from a specific config file
|
|
956
|
+
$ jbrowse remove-track my_track_id --target /path/to/jb2/config.json
|
|
590
957
|
```
|
|
591
958
|
|
|
592
959
|
|
|
593
960
|
## jbrowse set-default-session
|
|
594
961
|
|
|
595
|
-
```
|
|
962
|
+
```text
|
|
596
963
|
Set a default session with views and tracks
|
|
597
964
|
|
|
598
|
-
Usage: jbrowse
|
|
965
|
+
Usage: jbrowse set-default-session [options]
|
|
599
966
|
|
|
600
967
|
Options:
|
|
601
968
|
-s, --session set path to a file containing session in json
|
|
602
|
-
format (required,
|
|
603
|
-
delete/currentSession flags)
|
|
969
|
+
format, or "-" to read it from stdin (required,
|
|
970
|
+
unless using delete/currentSession flags)
|
|
604
971
|
|
|
605
|
-
-n, --name Give a name for the default session
|
|
606
|
-
Default
|
|
972
|
+
-n, --name Give a name for the default session (overrides any
|
|
973
|
+
name in the session file; defaults to "New Default
|
|
974
|
+
Session")
|
|
607
975
|
|
|
608
976
|
-c, --currentSession List out the current default session
|
|
609
977
|
|
|
@@ -616,14 +984,25 @@ Options:
|
|
|
616
984
|
|
|
617
985
|
-h, --help Show help
|
|
618
986
|
|
|
987
|
+
Examples:
|
|
988
|
+
|
|
619
989
|
# set default session for the config.json in your current directory
|
|
620
990
|
$ jbrowse set-default-session --session /path/to/default/session.json
|
|
621
991
|
|
|
622
992
|
# make session.json the defaultSession on the specified target config.json file
|
|
623
993
|
$ jbrowse set-default-session --target /path/to/jb2/installation/config.json --session session.json
|
|
624
994
|
|
|
625
|
-
#
|
|
626
|
-
$ jbrowse set-default-session --
|
|
995
|
+
# override the name stored in the session file
|
|
996
|
+
$ jbrowse set-default-session --session session.json --name "My default view"
|
|
997
|
+
|
|
998
|
+
# pipe a session in from another program
|
|
999
|
+
$ jq '.defaultSession' other/config.json | jbrowse set-default-session --session -
|
|
1000
|
+
|
|
1001
|
+
# print the current default session
|
|
1002
|
+
$ jbrowse set-default-session --currentSession
|
|
1003
|
+
|
|
1004
|
+
# remove the existing default session
|
|
1005
|
+
$ jbrowse set-default-session --delete
|
|
627
1006
|
```
|
|
628
1007
|
|
|
629
1008
|
|