@gmod/bam 8.4.2 → 8.5.1

package/README.md

@@ -21,18 +21,51 @@ const records = await bam.getRecordsForRange('ctgA', 0, 50000)
 ```
 
 Coordinates are 0-based half-open (not the same as `samtools view` inputs).
-`bamPath` reads a local file, so it is node-only; in the browser pass a
-filehandle or URL instead:
+`bamPath` reads a local file, so it is node-only; in the browser pass a URL or a
+generic-filehandle2 filehandle instead:
 
 ```typescript
-import { BamFile } from '@gmod/bam'
-
 const bam = new BamFile({
   bamUrl: 'https://example.com/yourfile.bam',
   baiUrl: 'https://example.com/yourfile.bam.bai',
 })
 ```
 
+Records come back unfiltered, and are shared between overlapping queries — treat
+them as read-only. Filter them yourself with the flag helpers and `getTag`,
+which decodes one tag instead of all of them:
+
+```typescript
+const records = (await bam.getRecordsForRange('chr1', 0, 100000)).filter(
+  r => r.isProperlyPaired() && !r.isSecondary() && r.getTag('RG') === 'rg1',
+)
+```
+
+## Decompressing on a worker pool
+
+BGZF decompression is 70-90% of a cold query, and BGZF blocks are independently
+inflatable. Hand `BamFile` a
+[`@gmod/bgzf-filehandle`](https://github.com/GMOD/bgzf-filehandle) worker pool
+and it inflates chunks there instead of on the calling thread — measured
+2.7-4.1x on the pool's own fixtures.
+
+```typescript
+import { getSharedWorkerPool } from '@gmod/bgzf-filehandle'
+
+const bam = new BamFile({
+  bamUrl: 'https://example.com/yourfile.bam',
+  // the pending promise is fine — it is awaited at the point of use
+  bgzfWorkerPool: getSharedWorkerPool(),
+})
+```
+
+No cross-origin isolation is needed. `getSharedWorkerPool()` gives back
+`undefined` under node, or anywhere Workers cannot be created, which keeps the
+in-process path — so this is safe to pass unconditionally. bam-js never creates
+a pool on its own: the thread budget belongs to the consumer. For worker counts,
+lifecycle and the pool's own benchmarks, see
+[bgzf-filehandle's worker pool docs](https://github.com/GMOD/bgzf-filehandle/blob/main/docs/worker-pool.md).
+
 ## Usage with htsget
 
 ```typescript
@@ -46,10 +79,8 @@ const records = await bam.getRecordsForRange('1', 2000000, 2000001)
 ```
 
 htsget fetches the server's range as-is, so `viewAsPairs`, `pairAcrossChr` and
-`maxInsertSize` are ignored.
-
-For a server that requires authentication, pass a `fetch` that adds the bearer
-token the spec calls for:
+`maxInsertSize` are ignored. For a server that requires authentication, pass a
+`fetch` that adds the bearer token:
 
 ```typescript
 const bam = new HtsgetFile({
@@ -63,227 +94,19 @@ const bam = new HtsgetFile({
 })
 ```
 
-Your `fetch` is called for the ticket request and for the data-block urls the
-ticket points at, so only attach credentials to hosts you trust — data blocks
-may live on a third-party host, and the spec has servers put whatever those need
-in each url's own `headers` field, which is applied either way.
-
-## Documentation
-
-### BamFile constructor
-
-- `bamPath`/`bamUrl`/`bamFilehandle` - local path, remote URL, or a
-  generic-filehandle2 object
-- `baiPath`/`baiUrl`/`baiFilehandle` - BAI index. Defaults to the `.bai` sibling
-  of `bamPath`/`bamUrl`
-- `csiPath`/`csiUrl`/`csiFilehandle` - CSI index, required for chromosomes
-  longer than 2^29
-- `renameRefSeqs` - `(refName: string) => string` applied to header ref names
-- `recordClass` - custom class extending `BamRecord` (see below)
-- `maxCacheBytes` - ceiling for the parsed-chunk cache, in decompressed bytes.
-  default: 1GB, `Infinity` for none. See [Caching](#caching)
-- `cacheIdleTimeoutMs` - drop a cached chunk once nothing has read it for this
-  long. default: 3 minutes; `0` disables it. See [Caching](#caching)
-
-The `path`/`url` forms are convenience wrappers for generic-filehandle2's
-`LocalFile` and `RemoteFile`.
-
-### Caching
-
-Parsed chunks — the unit the BAM index hands out — are cached, so overlapping
-and adjacent queries reuse decompressed records instead of re-fetching them. Two
-options bound that cache, and they answer different questions.
-
-**`maxCacheBytes` is a ceiling under load, not a limit on what you can ask
-for.** Nothing is ever refused for being too large: a chunk bigger than the
-whole budget is still cached, reads in flight are never evicted, and eviction
-only drops a value that has already been returned once. The worst a budget can
-cost you is a re-read. It can make a query slower; it can never make one fail or
-come back short.
-
-**It binds less often than its size suggests.** On the deepest data we measure —
-1000x coverage long reads, 240 windows over six laps — the cache settles at
-573MB across 60 entries and eviction never runs at the 1GB default. Treat it as
-a backstop against a session that pans forever, not as an operating constraint.
-
-**Don't pick a number between one query and several.** Below one query's working
-set the cache inverts: each chunk is evicted before the next pan can reuse it,
-so the hit rate is zero, the full re-decompress is paid every time, and the
-unevictable entries retain the memory anyway. At a 200MB budget on that same
-file the cache holds exactly one entry, because one chunk there decompresses to
-181MB. Either size it above the working set or pass `Infinity` and bound memory
-some other way.
-
-**`cacheIdleTimeoutMs` is the only thing that gives memory back.**
-`maxCacheBytes` is enforced when a read settles, so an idle cache sits at
-whatever it reached and never lowers — and for a page that holds a `BamFile` for
-the life of a track, that resting level is the number that actually matters. The
-idle sweep is what makes a generous ceiling affordable, by turning it into a
-peak under panning rather than a level a parked tab holds indefinitely. The
-clock runs from the last _read_ of a chunk, or from its parse landing if nothing
-has read it since, so panning back and forth over one region never expires it
-and a slow chunk still gets the full timeout to be reused in. Measured on a pan
-that held 331MB: 0MB once idle.
-
-**Neither bounds peak memory**, and on deep data the gap is not small:
-
-- Up to six chunk reads run at once and an in-flight read is never evicted. At
-  181MB a chunk, that is ~476MB in flight before retention holds anything.
-- A query holds every chunk it parsed until it returns, whether or not the cache
-  still does.
-- An entry is weighed once, when its read settles, and records grow after that.
-  `end`, `CIGAR` and `tags` each memoize onto the record the first time they are
-  read, which is what a renderer does to every visible read — measured at +38%
-  over the weighed size.
-
-So these are bounds on retained decompressed bytes, not on the heap. Size
-against what you want to keep, and bound total memory at a level that can see
-the whole process.
-
-### HtsgetFile constructor
-
-- `baseUrl` - htsget reads endpoint, e.g. `https://htsget.example.com/reads`
-- `trackId` - id of the resource under `baseUrl`
-- `fetch` - `fetch` replacement for adding auth headers (see above)
-- `recordClass` - custom class extending `BamRecord` (see below)
-
-### async getRecordsForRange(refName, start, end, opts?)
-
-- `refName` - chromosome to fetch from
-- `start`/`end` - 0-based half-open coordinates
-- `opts.signal` - `AbortSignal` to stop processing
-- `opts.viewAsPairs` - re-dispatch requests to find mate pairs. default: false
-- `opts.pairAcrossChr` - let `viewAsPairs` pair across chromosomes. default:
-  false
-- `opts.maxInsertSize` - distance limit for `viewAsPairs` within a chromosome.
-  default: 200kb
-- `opts.onProgress` - `(bytesDownloaded, totalBytes?) => void`, called per BGZF
-  chunk for a determinate progress bar
-
-Returned records are cached and shared between overlapping queries, so treat
-them as read-only — attaching your own fields to a record mutates it for every
-other query holding it.
-
-Records come back unfiltered. Filter them yourself with the flag helpers and
-`getTag`, which decodes one tag instead of all of them:
-
-```typescript
-const records = (await bam.getRecordsForRange('chr1', 0, 100000)).filter(
-  r => r.isProperlyPaired() && !r.isSecondary() && r.getTag('RG') === 'rg1',
-)
-```
-
-### async getHeader(opts?)
-
-Returns the parsed SAM header. Called automatically by the query methods and
-cached, so you only need it when you want the header itself.
-`getHeaderText(opts?)` returns the raw header string.
-
-### async indexCov(refName, start?, end?)
-
-Returns `{start, end, score}` features estimating read density over 16kb
-windows, derived from the BAI linear index. CSI has no linear index, so a
-CSI-indexed file returns `[]`.
+Your `fetch` is called for the ticket request _and_ for the data-block urls the
+ticket points at, which may live on a third-party host — so only attach
+credentials to hosts you trust.
 
-### async lineCount(refName)
-
-Number of records on `refName` from the index's pseudo-bin (bin 37450 in BAI,
-`n_mapped` in the SAM spec), or 0 if `refName` is absent.
-
-### async hasRefSeq(refName)
-
-Whether `refName` is present in the file.
-
-### async estimatedBytesForRegions(regions, opts?)
-
-Compressed bytes the given `{refName, start, end}[]` would fetch — useful for
-warning before a large query.
-
-### clearFeatureCache()
-
-Drops the parsed-chunk cache immediately, rather than waiting for
-`maxCacheBytes` or `cacheIdleTimeoutMs` to reclaim it. See [Caching](#caching).
-
-### BamRecord
-
-```typescript
-// Core alignment fields
-record.fileOffset // "file offset" based id -- not a true file offset
-record.ref_id // numerical sequence id from SAM header
-record.start // 0-based start coordinate
-record.end // 0-based end coordinate
-record.name // QNAME
-record.seq // sequence string
-record.qual // Uint8Array of quality scores (null if SEQ is empty)
-record.CIGAR // CIGAR string e.g. "50M2I48M"
-record.flags // SAM flags integer
-record.mq // mapping quality (undefined if 255)
-record.strand // 1 or -1
-record.template_length // TLEN
+## Docs
 
-// Mate info
-record.next_refid
-record.next_pos
-
-// Auxiliary data
-record.tags // all aux tags e.g. {MD: "100", NM: 0}
-record.getTag('MD') // one tag, without decoding the rest
-record.getTagRaw('MD') // string tag as Uint8Array, skipping string conversion
-
-// Typed-array views, for rendering without allocating strings
-record.NUMERIC_MD // MD tag as Uint8Array
-record.NUMERIC_CIGAR // Uint32Array of packed CIGAR operations
-record.NUMERIC_SEQ // Uint8Array of 4-bit encoded sequence
-
-// Flag methods
-record.isPaired()
-record.isProperlyPaired()
-record.isSegmentUnmapped()
-record.isMateUnmapped()
-record.isReverseComplemented()
-record.isMateReverseComplemented()
-record.isRead1()
-record.isRead2()
-record.isSecondary()
-record.isFailedQc()
-record.isDuplicate()
-record.isSupplementary()
-
-// Utility
-record.seqAt(idx) // single base at position
-record.toJSON()
-```
-
-### Custom BamRecord class
-
-```typescript
-import { BamFile, BamRecord } from '@gmod/bam'
-
-class CustomBamRecord extends BamRecord {
-  get customProperty() {
-    return `custom-${this.name}`
-  }
-}
-
-const bam = new BamFile<CustomBamRecord>({
-  bamPath: 'test.bam',
-  recordClass: CustomBamRecord,
-})
-
-// records are typed as CustomBamRecord[]
-const records = await bam.getRecordsForRange('ctgA', 0, 50000)
-console.log(records[0].customProperty)
-```
+- [docs/api.md](docs/api.md) — every constructor option, method and `BamRecord`
+  field, plus custom record classes
+- [docs/caching.md](docs/caching.md) — sizing the parsed-chunk cache
+- [agent-docs/adr/](agent-docs/adr/) — the measurements behind the performance
+  and caching decisions
+- [CONTRIBUTING.md](CONTRIBUTING.md) — development and release steps
 
 ## License
 
 MIT © [Colin Diesh](https://github.com/cmdcolin)
-
-## Publishing
-
-[Trusted publishing](https://docs.npmjs.com/about-trusted-publishing) via GitHub
-Actions.
-
-```bash
-pnpm version patch  # or minor/major
-```

package/dist/bamFile.d.ts

@@ -4,6 +4,7 @@ import CSI from './csi.ts';
 import BAMFeature from './record.ts';
 import type Chunk from './chunk.ts';
 import type { BamOpts, BaseOpts } from './util.ts';
+import type { BgzfWorkerPool } from '@gmod/bgzf-filehandle';
 import type { SharedBudget } from '@gmod/shared-read-cache';
 import type { GenericFilehandle } from 'generic-filehandle2';
 export interface BamRecordLike {
@@ -43,7 +44,9 @@ export default class BamFile<T extends BamRecordLike = BAMFeature> {
     private headerCache;
     chunkFeatureCache: SharedReadCache<Chunk, ChunkEntry<T>>;
     private RecordClass;
-    constructor({ bamFilehandle, bamPath, bamUrl, baiPath, baiFilehandle, baiUrl, csiPath, csiFilehandle, csiUrl, htsget, renameRefSeqs, recordClass, maxCacheBytes, cacheIdleTimeoutMs, cacheBudget, }: {
+    /** see the constructor option of the same name */
+    private bgzfWorkerPool?;
+    constructor({ bamFilehandle, bamPath, bamUrl, baiPath, baiFilehandle, baiUrl, csiPath, csiFilehandle, csiUrl, htsget, renameRefSeqs, recordClass, maxCacheBytes, cacheIdleTimeoutMs, cacheBudget, bgzfWorkerPool, }: {
         bamFilehandle?: GenericFilehandle;
         bamPath?: string;
         bamUrl?: string;
@@ -116,6 +119,22 @@ export default class BamFile<T extends BamRecordLike = BAMFeature> {
          * space to the one being panned.
          */
         cacheBudget?: SharedBudget;
+        /**
+         * A `@gmod/bgzf-filehandle` worker pool to inflate this file's chunks on,
+         * instead of inflating them on the calling thread.
+         *
+         * BGZF decompression is 70-90% of a cold query (ADR 0003), and BGZF blocks
+         * are independently inflatable, so this is the one remaining lever of that
+         * size. Measured 2.7-4.1x on the pool's own fixtures.
+         *
+         * Accepts the promise `getSharedWorkerPool()` returns as well as a pool,
+         * so a caller whose own construction is synchronous can hand the pending
+         * pool straight over — it is awaited at the point of use, by which time it
+         * has long since resolved. `undefined` (which is what that helper gives
+         * back under node, or anywhere Workers cannot be created) keeps the
+         * in-process path, so this is safe to pass unconditionally.
+         */
+        bgzfWorkerPool?: BgzfWorkerPool | Promise<BgzfWorkerPool | undefined> | undefined;
     });
     getHeaderPre(opts?: BaseOpts): Promise<{
         tag: string;

package/dist/bamFile.js

@@ -110,8 +110,11 @@ class BamFile {
     // than racing to decompress it twice.
     chunkFeatureCache;
     RecordClass;
-    constructor({ bamFilehandle, bamPath, bamUrl, baiPath, baiFilehandle, baiUrl, csiPath, csiFilehandle, csiUrl, htsget, renameRefSeqs = n => n, recordClass, maxCacheBytes = exports.DEFAULT_MAX_CACHE_BYTES, cacheIdleTimeoutMs = exports.DEFAULT_CACHE_IDLE_TIMEOUT_MS, cacheBudget, }) {
+    /** see the constructor option of the same name */
+    bgzfWorkerPool;
+    constructor({ bamFilehandle, bamPath, bamUrl, baiPath, baiFilehandle, baiUrl, csiPath, csiFilehandle, csiUrl, htsget, renameRefSeqs = n => n, recordClass, maxCacheBytes = exports.DEFAULT_MAX_CACHE_BYTES, cacheIdleTimeoutMs = exports.DEFAULT_CACHE_IDLE_TIMEOUT_MS, cacheBudget, bgzfWorkerPool, }) {
         this.renameRefSeq = renameRefSeqs;
+        this.bgzfWorkerPool = bgzfWorkerPool;
         this.RecordClass = (recordClass ?? record_ts_1.default);
         this.chunkFeatureCache = new shared_read_cache_1.SharedReadCache({
             maxSize: maxCacheBytes,
@@ -466,7 +469,11 @@ class BamFile {
         // caller left to be denied the result. @gmod/cram checks in the same place
         // and for the same reason, before the decode loop in `_fetchRecords`.
         (0, util_ts_1.throwIfAborted)(opts.signal);
-        const { buffer: data, cpositions, dpositions, } = await (0, bgzf_filehandle_1.unzipChunkSlice)(buf, chunk);
+        // Awaited rather than stored resolved: the pool is commonly handed over
+        // as the pending promise from getSharedWorkerPool(), and awaiting an
+        // already-settled promise costs a microtask.
+        const pool = await this.bgzfWorkerPool;
+        const { buffer: data, cpositions, dpositions, } = await (0, bgzf_filehandle_1.unzipChunkSlice)(buf, chunk, pool);
         return {
             features: this.readBamFeatures(data, cpositions, dpositions, chunk),
             bytes: data.byteLength,

package/dist/bamFile.js.map

@@ -1 +1 @@
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package/esm/bamFile.d.ts

@@ -4,6 +4,7 @@ import CSI from './csi.ts';
 import BAMFeature from './record.ts';
 import type Chunk from './chunk.ts';
 import type { BamOpts, BaseOpts } from './util.ts';
+import type { BgzfWorkerPool } from '@gmod/bgzf-filehandle';
 import type { SharedBudget } from '@gmod/shared-read-cache';
 import type { GenericFilehandle } from 'generic-filehandle2';
 export interface BamRecordLike {
@@ -43,7 +44,9 @@ export default class BamFile<T extends BamRecordLike = BAMFeature> {
     private headerCache;
     chunkFeatureCache: SharedReadCache<Chunk, ChunkEntry<T>>;
     private RecordClass;
-    constructor({ bamFilehandle, bamPath, bamUrl, baiPath, baiFilehandle, baiUrl, csiPath, csiFilehandle, csiUrl, htsget, renameRefSeqs, recordClass, maxCacheBytes, cacheIdleTimeoutMs, cacheBudget, }: {
+    /** see the constructor option of the same name */
+    private bgzfWorkerPool?;
+    constructor({ bamFilehandle, bamPath, bamUrl, baiPath, baiFilehandle, baiUrl, csiPath, csiFilehandle, csiUrl, htsget, renameRefSeqs, recordClass, maxCacheBytes, cacheIdleTimeoutMs, cacheBudget, bgzfWorkerPool, }: {
         bamFilehandle?: GenericFilehandle;
         bamPath?: string;
         bamUrl?: string;
@@ -116,6 +119,22 @@ export default class BamFile<T extends BamRecordLike = BAMFeature> {
          * space to the one being panned.
          */
         cacheBudget?: SharedBudget;
+        /**
+         * A `@gmod/bgzf-filehandle` worker pool to inflate this file's chunks on,
+         * instead of inflating them on the calling thread.
+         *
+         * BGZF decompression is 70-90% of a cold query (ADR 0003), and BGZF blocks
+         * are independently inflatable, so this is the one remaining lever of that
+         * size. Measured 2.7-4.1x on the pool's own fixtures.
+         *
+         * Accepts the promise `getSharedWorkerPool()` returns as well as a pool,
+         * so a caller whose own construction is synchronous can hand the pending
+         * pool straight over — it is awaited at the point of use, by which time it
+         * has long since resolved. `undefined` (which is what that helper gives
+         * back under node, or anywhere Workers cannot be created) keeps the
+         * in-process path, so this is safe to pass unconditionally.
+         */
+        bgzfWorkerPool?: BgzfWorkerPool | Promise<BgzfWorkerPool | undefined> | undefined;
     });
     getHeaderPre(opts?: BaseOpts): Promise<{
         tag: string;

package/esm/bamFile.js

@@ -104,8 +104,11 @@ export default class BamFile {
     // than racing to decompress it twice.
     chunkFeatureCache;
     RecordClass;
-    constructor({ bamFilehandle, bamPath, bamUrl, baiPath, baiFilehandle, baiUrl, csiPath, csiFilehandle, csiUrl, htsget, renameRefSeqs = n => n, recordClass, maxCacheBytes = DEFAULT_MAX_CACHE_BYTES, cacheIdleTimeoutMs = DEFAULT_CACHE_IDLE_TIMEOUT_MS, cacheBudget, }) {
+    /** see the constructor option of the same name */
+    bgzfWorkerPool;
+    constructor({ bamFilehandle, bamPath, bamUrl, baiPath, baiFilehandle, baiUrl, csiPath, csiFilehandle, csiUrl, htsget, renameRefSeqs = n => n, recordClass, maxCacheBytes = DEFAULT_MAX_CACHE_BYTES, cacheIdleTimeoutMs = DEFAULT_CACHE_IDLE_TIMEOUT_MS, cacheBudget, bgzfWorkerPool, }) {
         this.renameRefSeq = renameRefSeqs;
+        this.bgzfWorkerPool = bgzfWorkerPool;
         this.RecordClass = (recordClass ?? BAMFeature);
         this.chunkFeatureCache = new SharedReadCache({
             maxSize: maxCacheBytes,
@@ -460,7 +463,11 @@ export default class BamFile {
         // caller left to be denied the result. @gmod/cram checks in the same place
         // and for the same reason, before the decode loop in `_fetchRecords`.
         throwIfAborted(opts.signal);
-        const { buffer: data, cpositions, dpositions, } = await unzipChunkSlice(buf, chunk);
+        // Awaited rather than stored resolved: the pool is commonly handed over
+        // as the pending promise from getSharedWorkerPool(), and awaiting an
+        // already-settled promise costs a microtask.
+        const pool = await this.bgzfWorkerPool;
+        const { buffer: data, cpositions, dpositions, } = await unzipChunkSlice(buf, chunk, pool);
         return {
             features: this.readBamFeatures(data, cpositions, dpositions, chunk),
             bytes: data.byteLength,

package/esm/bamFile.js.map

@@ -1 +1 @@
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package/package.json

@@ -1,6 +1,6 @@
 {
   "name": "@gmod/bam",
-  "version": "8.4.2",
+  "version": "8.5.1",
   "packageManager": "pnpm@11.15.1",
   "description": "Parser for BAM and BAM index (bai) files",
   "license": "MIT",
@@ -51,7 +51,7 @@
     "genomics"
   ],
   "dependencies": {
-    "@gmod/bgzf-filehandle": "^6.3.2",
+    "@gmod/bgzf-filehandle": "^6.4.0",
     "@gmod/shared-read-cache": "^1.5.1",
     "@jbrowse/quick-lru": "^7.3.5",
     "crc": "^4.3.2",

package/src/bamFile.ts

@@ -17,6 +17,7 @@ import {
 
 import type Chunk from './chunk.ts'
 import type { BamOpts, BaseOpts } from './util.ts'
+import type { BgzfWorkerPool } from '@gmod/bgzf-filehandle'
 import type { SharedBudget } from '@gmod/shared-read-cache'
 import type { GenericFilehandle } from 'generic-filehandle2'
 
@@ -169,6 +170,10 @@ export default class BamFile<T extends BamRecordLike = BAMFeature> {
 
   private RecordClass: BamRecordClass<T>
 
+  /** see the constructor option of the same name */
+  private bgzfWorkerPool?:
+    BgzfWorkerPool | Promise<BgzfWorkerPool | undefined> | undefined
+
   constructor({
     bamFilehandle,
     bamPath,
@@ -185,6 +190,7 @@ export default class BamFile<T extends BamRecordLike = BAMFeature> {
     maxCacheBytes = DEFAULT_MAX_CACHE_BYTES,
     cacheIdleTimeoutMs = DEFAULT_CACHE_IDLE_TIMEOUT_MS,
     cacheBudget,
+    bgzfWorkerPool,
   }: {
     bamFilehandle?: GenericFilehandle
     bamPath?: string
@@ -258,8 +264,26 @@ export default class BamFile<T extends BamRecordLike = BAMFeature> {
      * space to the one being panned.
      */
     cacheBudget?: SharedBudget
+    /**
+     * A `@gmod/bgzf-filehandle` worker pool to inflate this file's chunks on,
+     * instead of inflating them on the calling thread.
+     *
+     * BGZF decompression is 70-90% of a cold query (ADR 0003), and BGZF blocks
+     * are independently inflatable, so this is the one remaining lever of that
+     * size. Measured 2.7-4.1x on the pool's own fixtures.
+     *
+     * Accepts the promise `getSharedWorkerPool()` returns as well as a pool,
+     * so a caller whose own construction is synchronous can hand the pending
+     * pool straight over — it is awaited at the point of use, by which time it
+     * has long since resolved. `undefined` (which is what that helper gives
+     * back under node, or anywhere Workers cannot be created) keeps the
+     * in-process path, so this is safe to pass unconditionally.
+     */
+    bgzfWorkerPool?:
+      BgzfWorkerPool | Promise<BgzfWorkerPool | undefined> | undefined
   }) {
     this.renameRefSeq = renameRefSeqs
+    this.bgzfWorkerPool = bgzfWorkerPool
     this.RecordClass = (recordClass ?? BAMFeature) as BamRecordClass<T>
     this.chunkFeatureCache = new SharedReadCache<Chunk, ChunkEntry<T>>({
       maxSize: maxCacheBytes,
@@ -687,11 +711,15 @@ export default class BamFile<T extends BamRecordLike = BAMFeature> {
     // and for the same reason, before the decode loop in `_fetchRecords`.
     throwIfAborted(opts.signal)
 
+    // Awaited rather than stored resolved: the pool is commonly handed over
+    // as the pending promise from getSharedWorkerPool(), and awaiting an
+    // already-settled promise costs a microtask.
+    const pool = await this.bgzfWorkerPool
     const {
       buffer: data,
       cpositions,
       dpositions,
-    } = await unzipChunkSlice(buf, chunk)
+    } = await unzipChunkSlice(buf, chunk, pool)
     return {
       features: this.readBamFeatures(data, cpositions, dpositions, chunk),
       bytes: data.byteLength,