viral_seq 1.10.2 → 1.10.3

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checksums.yaml CHANGED
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data/README.md CHANGED
@@ -191,6 +191,11 @@ qc_seqhash.sdrm_hiv_pr(cut_off)
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  ## Updates
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+ ### Version-1.10.3-12112025
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+
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+ 1. Bug fix for SDRM pipeline.
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+ 2. Add a function in `locator` tool to export sequences in positive sense direction.
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+
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  ### Version-1.10.2-07210225
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  1. Fixed a bug processing parameters for HIV sequence QC.
data/bin/locator CHANGED
@@ -76,6 +76,10 @@ begin
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  else
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  csv_file = seq_file + ".csv"
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  end
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+
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+ fasta_file = csv_file.sub(/\.csv$/i, '.direction.fasta')
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+
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+ fasta_handle = File.open(fasta_file, 'w')
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  unless File.exist?(seq_file)
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  raise StandardError.new("Input file sequence file not found".red.bold)
@@ -92,9 +96,19 @@ begin
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  locs = seqs.loc(opt)
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  head = ["title", "sequence", "ref", "direction", "start", "end", "similarity", "indel", "aligned_input", "aligned_ref"]
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  locs.unshift(head)
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+
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+ directional_fasta = []
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  data = CSV.generate do |csv|
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- locs.each {|loc| csv << loc}
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+ locs.each do |loc|
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+ csv << loc
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+ directional_fasta << loc[1]
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+ directional_fasta << loc[8].tr("-", "") # remove gaps for aligned_input
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+ end
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+ end
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+ directional_fasta[2..-1].each do |line|
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+ fasta_handle.puts line
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  end
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+ fasta_handle.close
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  File.write(csv_file, data)
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  puts "Output file found at #{csv_file.green.bold}"
data/bin/tcs_sdrm CHANGED
@@ -12,10 +12,10 @@
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  # ├── lib1_IN
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  # ├── lib1_V1V3
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  # ├── lib2
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- # ├── lib1_RT
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- # ├── lib1_PR
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- # ├── lib1_IN
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- # ├── lib1_V1V3
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+ # ├── lib2_RT
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+ # ├── lib2_PR
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+ # ├── lib2_IN
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+ # ├── lib2_V1V3
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  # ├── ...
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  #
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  # output data in a new dir as 'libs_dir_SDRM'
@@ -37,7 +37,7 @@ r_version = ViralSeq::R.check_R
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  ViralSeq::R.check_R_packages
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  def abstract_line(data)
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- return_data = data[3] + data[2] + data[4] + ":" +
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+ data[3] + data[2] + data[4] + ":" +
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  (data[6].to_f * 100).round(2).to_s + "(" +
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  (data[7].to_f * 100).round(2).to_s + "-" +
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  (data[8].to_f * 100).round(2).to_s + "); "
@@ -143,7 +143,7 @@ libs.each do |lib|
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  seq_basename.gsub!(/\_P17/i, "_CA")
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  region = version_config.query_region(region_name.to_s)
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- puts "prcessing region: " + region.region
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+ puts "processing region: " + region.region
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  sh = ViralSeq::SeqHash.fa(path_to_file)
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@@ -492,7 +492,7 @@ module ViralSeq
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  self.dna_hash.each do |k,v|
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  r1_seqs[k] = v[0,r1_length]
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- r2_seqs[k] = v[r1_length, r2_length]
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+ r2_seqs[k] = v[-r2_length..-1] # to ensure the length from the end. Sometimes the platform will return sequence with one extra base.
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  end
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  r1_sh = ViralSeq::SeqHash.new(r1_seqs)
@@ -106,7 +106,7 @@ module ViralSeq
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  )
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  formatted_text(
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- text_format2("P17", log[:pi_P17], log[:dist20_P17], log[:tcs_P17])
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+ text_format2("CA", log[:pi_CA], log[:dist20_CA], log[:tcs_CA])
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  )
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  move_down 30
@@ -250,7 +250,7 @@ module ViralSeq
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  private
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  # determine overlap size from @dna_hash
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- def determine_overlap_pid_pair(seq_pair_hash, diff = 0.0)
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+ def determine_overlap_pid_pair(seq_pair_hash, diff = 0.02)
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  overlaps = []
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  seq_pair_hash.each do |_seq_name, seq_pair|
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  overlap_list = []
@@ -1,684 +1,429 @@
1
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  {
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- "HCV_NS5A": [
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- {
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- "position": 28,
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- "wild-type": "M",
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- },
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- {
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- ]
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- ]
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- },
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- {
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- "position": 85,
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- "N",
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- "Y"
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- ]
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- },
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- {
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- {
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- {
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- {
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- {
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- {
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- "position": 75,
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- {
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- "position": 115,
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- "wild-type": "Y",
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- "mutations": [
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- "F"
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- ]
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- },
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- {
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- "position": 116,
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- "wild-type": "F",
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- "mutations": [
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- "Y"
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- ]
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- },
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- {
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- "position": 151,
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- },
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- {
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- "position": 184,
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- "wild-type": "M",
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- "mutations": [
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- "V",
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- "I"
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- ]
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- },
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- {
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- "position": 210,
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- "wild-type": "L",
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- "mutations": [
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- "W"
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- ]
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- },
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- {
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- "position": 215,
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- "wild-type": "T",
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- "mutations": [
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- "Y",
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- "F",
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- "I",
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- "C",
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- "D",
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- "V",
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- "E"
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- ]
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- },
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- {
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- "position": 219,
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- "wild-type": "K",
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- "mutations": [
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- "Q",
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- "E",
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- "N",
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- "R"
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- ]
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- }
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- ],
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- "NNRTI": [
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- {
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- "position": 98,
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- "wild-type": "A",
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- "mutations": [
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- "G"
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- ]
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- },
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- {
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- "position": 100,
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- "wild-type": "L",
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- "mutations": [
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- "I"
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- ]
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- },
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- {
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- "position": 101,
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- "wild-type": "K",
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- "mutations": [
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- "E",
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- "P"
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- ]
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- },
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- {
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- "position": 103,
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- "wild-type": "K",
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- "mutations": [
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- "N",
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- "S"
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- ]
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- },
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- {
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- "position": 106,
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- "wild-type": "V",
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- "mutations": [
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- ]
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- },
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- {
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- "A",
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- "G",
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- ]
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- },
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- {
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- "position": 179,
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- "F",
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- "D"
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- ]
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- },
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- {
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- "position": 181,
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- "wild-type": "Y",
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- "mutations": [
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- "C",
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- "I",
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- "V"
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- ]
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- },
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- {
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- "position": 188,
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- "wild-type": "Y",
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- "mutations": [
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- "L",
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- "H",
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- "C"
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- ]
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- },
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- {
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- "position": 190,
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- "wild-type": "G",
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- "mutations": [
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- "A",
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- "S",
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- "E",
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- "C",
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- "T",
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- "V"
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- ]
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- },
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- {
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- "position": 225,
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- "wild-type": "P",
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- "mutations": [
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- "H"
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- ]
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- },
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- {
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- "position": 230,
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- "wild-type": "M",
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- "mutations": [
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- "L"
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- ]
368
- }
369
- ],
370
- "PI": [
371
- {
372
- "position": 23,
373
- "wild-type": "L",
374
- "mutations": [
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- "I"
376
- ]
377
- },
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- {
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- "position": 24,
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- "wild-type": "L",
381
- "mutations": [
382
- "I"
383
- ]
384
- },
385
- {
386
- "position": 30,
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- "wild-type": "D",
388
- "mutations": [
389
- "N"
390
- ]
391
- },
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- {
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- "position": 32,
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- "mutations": [
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- "I"
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- ]
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- {
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- "position": 46,
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- "mutations": [
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- "I",
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- "L"
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- ]
406
- },
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- {
408
- "position": 47,
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- "wild-type": "I",
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- "mutations": [
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- "V",
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- ]
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- {
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- "position": 48,
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- ]
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- ]
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461
- "position": 76,
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- "V"
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- "position": 83,
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- "D"
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- {
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- "position": 84,
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490
- "mutations": [
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496
- {
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- "S"
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- ]
503
- },
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- {
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- "position": 90,
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- "wild-type": "L",
507
- "mutations": [
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- ]
510
- }
511
- ],
512
- "INSTI": [
513
- {
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- "position": 66,
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- "mutations": [
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- "A",
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- "I",
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- ]
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- "position": 74,
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541
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- "position": 97,
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- {
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- "position": 143,
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569
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- {
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- "position": 147,
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579
- "G"
580
- ]
581
- },
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- {
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- "position": 148,
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- "wild-type": "Q",
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590
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- {
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- "position": 263,
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595
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597
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599
- "position": 155,
600
- "wild-type": [
601
- "N",
602
- [
603
- "S",
604
- "H"
605
- ]
606
- ],
607
- "mutations": [
608
- "R",
609
- [
610
- "K"
611
- ]
612
- ]
613
- }
614
- ],
615
- "CAI": [
616
- {
617
- "position": 56,
618
- "wild-type": "L",
619
- "mutations": [
620
- "I"
621
- ]
622
- },
623
- {
624
- "position": 57,
625
- "wild-type": "N",
626
- "mutations": [
627
- "S"
628
- ]
629
- },
630
- {
631
- "position": 66,
632
- "wild-type": "M",
633
- "mutations": [
634
- "I"
635
- ]
636
- },
637
- {
638
- "position": 67,
639
- "wild-type": "Q",
640
- "mutations": [
641
- "H",
642
- "Y",
643
- "N",
644
- "K"
645
- ]
646
- },
647
- {
648
- "position": 70,
649
- "wild-type": "K",
650
- "mutations": [
651
- "N",
652
- "H",
653
- "R",
654
- "S"
655
- ]
656
- },
657
- {
658
- "position": 74,
659
- "wild-type": "N",
660
- "mutations": [
661
- "D",
662
- "S"
663
- ]
664
- },
665
- {
666
- "position": 105,
667
- "wild-type": "A",
668
- "mutations": [
669
- "T",
670
- "S",
671
- "E"
672
- ]
673
- },
674
- {
675
- "position": 107,
676
- "wild-type": "T",
677
- "mutations": [
678
- "N",
679
- "S",
680
- "A"
681
- ]
682
- }
683
- ]
2
+ "HCV_NS5A": [
3
+ {
4
+ "position": 28,
5
+ "wild-type": "M",
6
+ "mutations": ["T"]
7
+ },
8
+ {
9
+ "position": 30,
10
+ "wild-type": "L",
11
+ "mutations": ["H", "K", "R", "Q", "A", "S", "D"]
12
+ },
13
+ {
14
+ "position": 31,
15
+ "wild-type": "L",
16
+ "mutations": ["M", "V", "F"]
17
+ },
18
+ {
19
+ "position": 32,
20
+ "wild-type": "P",
21
+ "mutations": ["L"]
22
+ },
23
+ {
24
+ "position": 44,
25
+ "wild-type": "K",
26
+ "mutations": ["R"]
27
+ },
28
+ {
29
+ "position": 58,
30
+ "wild-type": "H",
31
+ "mutations": ["D", "P", "S"]
32
+ },
33
+ {
34
+ "position": 64,
35
+ "wild-type": "T",
36
+ "mutations": ["A", "S"]
37
+ },
38
+ {
39
+ "position": 77,
40
+ "wild-type": "P",
41
+ "mutations": ["A", "S"]
42
+ },
43
+ {
44
+ "position": 78,
45
+ "wild-type": "R",
46
+ "mutations": ["K"]
47
+ },
48
+ {
49
+ "position": 79,
50
+ "wild-type": "T",
51
+ "mutations": ["A"]
52
+ },
53
+ {
54
+ "position": 83,
55
+ "wild-type": "T",
56
+ "mutations": ["M"]
57
+ },
58
+ {
59
+ "position": 85,
60
+ "wild-type": "S",
61
+ "mutations": ["N", "H", "Y"]
62
+ },
63
+ {
64
+ "position": 92,
65
+ "wild-type": "A",
66
+ "mutations": ["P", "T", "K", "E"]
67
+ },
68
+ {
69
+ "position": 93,
70
+ "wild-type": "Y",
71
+ "mutations": ["C", "F", "H", "N"]
72
+ },
73
+ {
74
+ "position": 107,
75
+ "wild-type": "K",
76
+ "mutations": ["T", "S"]
77
+ },
78
+ {
79
+ "position": 121,
80
+ "wild-type": "I",
81
+ "mutations": ["V"]
82
+ },
83
+ {
84
+ "position": 135,
85
+ "wild-type": "T",
86
+ "mutations": ["A"]
87
+ }
88
+ ],
89
+ "NRTI": [
90
+ {
91
+ "position": 41,
92
+ "wild-type": "M",
93
+ "mutations": ["L"]
94
+ },
95
+ {
96
+ "position": 65,
97
+ "wild-type": "K",
98
+ "mutations": ["R"]
99
+ },
100
+ {
101
+ "position": 67,
102
+ "wild-type": "D",
103
+ "mutations": ["N", "G", "E"]
104
+ },
105
+ {
106
+ "position": 69,
107
+ "wild-type": "T",
108
+ "mutations": ["D"]
109
+ },
110
+ {
111
+ "position": 70,
112
+ "wild-type": "K",
113
+ "mutations": ["R", "E"]
114
+ },
115
+ {
116
+ "position": 74,
117
+ "wild-type": "L",
118
+ "mutations": ["V", "I"]
119
+ },
120
+ {
121
+ "position": 75,
122
+ "wild-type": "V",
123
+ "mutations": ["M", "T", "A", "S"]
124
+ },
125
+ {
126
+ "position": 77,
127
+ "wild-type": "F",
128
+ "mutations": ["L"]
129
+ },
130
+ {
131
+ "position": 115,
132
+ "wild-type": "Y",
133
+ "mutations": ["F"]
134
+ },
135
+ {
136
+ "position": 116,
137
+ "wild-type": "F",
138
+ "mutations": ["Y"]
139
+ },
140
+ {
141
+ "position": 151,
142
+ "wild-type": "Q",
143
+ "mutations": ["M"]
144
+ },
145
+ {
146
+ "position": 184,
147
+ "wild-type": "M",
148
+ "mutations": ["V", "I"]
149
+ },
150
+ {
151
+ "position": 210,
152
+ "wild-type": "L",
153
+ "mutations": ["W"]
154
+ },
155
+ {
156
+ "position": 215,
157
+ "wild-type": "T",
158
+ "mutations": ["Y", "F", "I", "C", "D", "V", "E"]
159
+ },
160
+ {
161
+ "position": 219,
162
+ "wild-type": "K",
163
+ "mutations": ["Q", "E", "N", "R"]
164
+ }
165
+ ],
166
+ "NNRTI": [
167
+ {
168
+ "position": 98,
169
+ "wild-type": "A",
170
+ "mutations": ["G"]
171
+ },
172
+ {
173
+ "position": 100,
174
+ "wild-type": "L",
175
+ "mutations": ["I"]
176
+ },
177
+ {
178
+ "position": 101,
179
+ "wild-type": "K",
180
+ "mutations": ["E", "P"]
181
+ },
182
+ {
183
+ "position": 103,
184
+ "wild-type": "K",
185
+ "mutations": ["N", "S"]
186
+ },
187
+ {
188
+ "position": 106,
189
+ "wild-type": "V",
190
+ "mutations": ["M", "A"]
191
+ },
192
+ {
193
+ "position": 138,
194
+ "wild-type": "E",
195
+ "mutations": ["A", "K", "G", "Q"]
196
+ },
197
+ {
198
+ "position": 179,
199
+ "wild-type": "V",
200
+ "mutations": ["F", "D"]
201
+ },
202
+ {
203
+ "position": 181,
204
+ "wild-type": "Y",
205
+ "mutations": ["C", "I", "V"]
206
+ },
207
+ {
208
+ "position": 188,
209
+ "wild-type": "Y",
210
+ "mutations": ["L", "H", "C"]
211
+ },
212
+ {
213
+ "position": 190,
214
+ "wild-type": "G",
215
+ "mutations": ["A", "S", "E", "C", "T", "V"]
216
+ },
217
+ {
218
+ "position": 225,
219
+ "wild-type": "P",
220
+ "mutations": ["H"]
221
+ },
222
+ {
223
+ "position": 230,
224
+ "wild-type": "M",
225
+ "mutations": ["L"]
226
+ }
227
+ ],
228
+ "PI": [
229
+ {
230
+ "position": 23,
231
+ "wild-type": "L",
232
+ "mutations": ["I"]
233
+ },
234
+ {
235
+ "position": 24,
236
+ "wild-type": "L",
237
+ "mutations": ["I"]
238
+ },
239
+ {
240
+ "position": 30,
241
+ "wild-type": "D",
242
+ "mutations": ["N"]
243
+ },
244
+ {
245
+ "position": 32,
246
+ "wild-type": "V",
247
+ "mutations": ["I"]
248
+ },
249
+ {
250
+ "position": 46,
251
+ "wild-type": "M",
252
+ "mutations": ["I", "L"]
253
+ },
254
+ {
255
+ "position": 47,
256
+ "wild-type": "I",
257
+ "mutations": ["V", "A"]
258
+ },
259
+ {
260
+ "position": 48,
261
+ "wild-type": "G",
262
+ "mutations": ["V", "M"]
263
+ },
264
+ {
265
+ "position": 50,
266
+ "wild-type": "I",
267
+ "mutations": ["V", "L"]
268
+ },
269
+ {
270
+ "position": 53,
271
+ "wild-type": "F",
272
+ "mutations": ["L"]
273
+ },
274
+ {
275
+ "position": 54,
276
+ "wild-type": "I",
277
+ "mutations": ["V", "L", "M", "T", "A", "S"]
278
+ },
279
+ {
280
+ "position": 73,
281
+ "wild-type": "G",
282
+ "mutations": ["S", "T", "C", "A"]
283
+ },
284
+ {
285
+ "position": 76,
286
+ "wild-type": "L",
287
+ "mutations": ["V"]
288
+ },
289
+ {
290
+ "position": 82,
291
+ "wild-type": "V",
292
+ "mutations": ["A", "T", "S", "F", "L", "C", "M"]
293
+ },
294
+ {
295
+ "position": 83,
296
+ "wild-type": "N",
297
+ "mutations": ["D"]
298
+ },
299
+ {
300
+ "position": 84,
301
+ "wild-type": "I",
302
+ "mutations": ["V", "A", "C"]
303
+ },
304
+ {
305
+ "position": 88,
306
+ "wild-type": "N",
307
+ "mutations": ["D", "S"]
308
+ },
309
+ {
310
+ "position": 90,
311
+ "wild-type": "L",
312
+ "mutations": ["M"]
313
+ }
314
+ ],
315
+ "INSTI": [
316
+ {
317
+ "position": 66,
318
+ "wild-type": "T",
319
+ "mutations": ["A", "I", "K"]
320
+ },
321
+ {
322
+ "position": 74,
323
+ "wild-type": "L",
324
+ "mutations": ["M"]
325
+ },
326
+ {
327
+ "position": 92,
328
+ "wild-type": "E",
329
+ "mutations": ["Q"]
330
+ },
331
+ {
332
+ "position": 95,
333
+ "wild-type": "Q",
334
+ "mutations": ["K"]
335
+ },
336
+ {
337
+ "position": 97,
338
+ "wild-type": "T",
339
+ "mutations": ["A"]
340
+ },
341
+ {
342
+ "position": 121,
343
+ "wild-type": "F",
344
+ "mutations": ["Y"]
345
+ },
346
+ {
347
+ "position": 140,
348
+ "wild-type": "G",
349
+ "mutations": ["A", "S", "C"]
350
+ },
351
+ {
352
+ "position": 143,
353
+ "wild-type": "Y",
354
+ "mutations": ["C", "H", "R"]
355
+ },
356
+ {
357
+ "position": 147,
358
+ "wild-type": "S",
359
+ "mutations": ["G"]
360
+ },
361
+ {
362
+ "position": 148,
363
+ "wild-type": "Q",
364
+ "mutations": ["H", "K", "R"]
365
+ },
366
+ {
367
+ "position": 263,
368
+ "wild-type": "R",
369
+ "mutations": ["K"]
370
+ },
371
+ {
372
+ "position": 155,
373
+ "wild-type": "N",
374
+ "mutations": ["S", "H", "T", "D"]
375
+ },
376
+ {
377
+ "position": 157,
378
+ "wild-type": "E",
379
+ "mutations": ["Q"]
380
+ },
381
+ {
382
+ "position": 163,
383
+ "wild-type": "G",
384
+ "mutations": ["R", "K"]
385
+ }
386
+ ],
387
+ "CAI": [
388
+ {
389
+ "position": 56,
390
+ "wild-type": "L",
391
+ "mutations": ["I"]
392
+ },
393
+ {
394
+ "position": 57,
395
+ "wild-type": "N",
396
+ "mutations": ["S"]
397
+ },
398
+ {
399
+ "position": 66,
400
+ "wild-type": "M",
401
+ "mutations": ["I"]
402
+ },
403
+ {
404
+ "position": 67,
405
+ "wild-type": "Q",
406
+ "mutations": ["H", "Y", "N", "K"]
407
+ },
408
+ {
409
+ "position": 70,
410
+ "wild-type": "K",
411
+ "mutations": ["N", "H", "R", "S"]
412
+ },
413
+ {
414
+ "position": 74,
415
+ "wild-type": "N",
416
+ "mutations": ["D", "S"]
417
+ },
418
+ {
419
+ "position": 105,
420
+ "wild-type": "A",
421
+ "mutations": ["T", "S", "E"]
422
+ },
423
+ {
424
+ "position": 107,
425
+ "wild-type": "T",
426
+ "mutations": ["N", "S", "A"]
427
+ }
428
+ ]
684
429
  }
@@ -2,6 +2,6 @@
2
2
  # version info and histroy
3
3
 
4
4
  module ViralSeq
5
- VERSION = "1.10.2"
6
- TCS_VERSION = "2.7.3"
5
+ VERSION = "1.10.3"
6
+ TCS_VERSION = "2.7.4"
7
7
  end
metadata CHANGED
@@ -1,7 +1,7 @@
1
1
  --- !ruby/object:Gem::Specification
2
2
  name: viral_seq
3
3
  version: !ruby/object:Gem::Version
4
- version: 1.10.2
4
+ version: 1.10.3
5
5
  platform: ruby
6
6
  authors:
7
7
  - Shuntai Zhou