transrate 1.0.0 → 1.0.1
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- checksums.yaml +4 -4
- data/.travis.yml +17 -0
- data/README.md +1 -1
- data/Rakefile +1 -1
- data/bin/transrate +2 -375
- data/docs/transrate_logo_full.png +0 -0
- data/files.txt +78 -0
- data/lib/transrate.rb +1 -0
- data/lib/transrate/assembly.rb +4 -0
- data/lib/transrate/cmdline.rb +543 -0
- data/lib/transrate/comparative_metrics.rb +3 -0
- data/lib/transrate/snap.rb +10 -0
- data/lib/transrate/version.rb +1 -1
- data/packaging/build_deps_linux.sh +57 -0
- data/packaging/build_deps_macosx.sh +55 -0
- data/packaging/bundler-config +3 -0
- data/packaging/minify.sh +43 -0
- data/packaging/transrate +20 -0
- data/test/data/test_contig_nc1.fa +2 -0
- data/test/data/test_contig_nc2.fa +4 -0
- data/test/data/test_contig_nc3.fa +6 -0
- data/test/data/test_contig_nc4.fa +4 -0
- data/test/data/test_contig_nc5.fa +6 -0
- data/test/data/test_contig_nc6.fa +2 -0
- data/test/data/test_contig_nc7.fa +2 -0
- data/test/data/test_reference_aa1.fa +2 -0
- data/test/data/test_reference_nc1.fa +2 -0
- data/test/helper.rb +35 -6
- data/test/test_assembly.rb +5 -3
- data/test/test_cmd.rb +1 -1
- data/test/test_cmdline.rb +114 -0
- data/test/test_comp_metrics.rb +2 -2
- data/test/test_contig.rb +1 -1
- data/test/test_contig_metrics.rb +1 -1
- data/test/test_inline.rb +1 -1
- data/test/test_optimiser.rb +16 -16
- data/test/test_read_metrics.rb +1 -1
- data/test/test_salmon.rb +1 -1
- data/test/test_snap.rb +1 -1
- data/test/test_transrate.rb +1 -1
- data/test/test_transrater.rb +2 -2
- data/test/vagrant/centos_6.5_64/Vagrantfile +122 -0
- data/test/vagrant/debian_7.4_64/Vagrantfile +126 -0
- data/test/vagrant/debian_7.4_64/provision.sh +28 -0
- data/test/vagrant/fedora_20_64/Vagrantfile +122 -0
- data/test/vagrant/fedora_20_64/provision.sh +16 -0
- data/test/vagrant/linux-x86_64/Vagrantfile +10 -0
- data/test/vagrant/osx/Vagrantfile +18 -0
- data/test/vagrant/ubuntu_12.04_64/Vagrantfile +126 -0
- data/test/vagrant/ubuntu_12.04_64/provision.sh +24 -0
- data/transrate.gemspec +2 -2
- metadata +38 -17
- data/test/test_bin.rb +0 -139
@@ -0,0 +1,16 @@
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# Setup
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sudo yum install curl
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# Install a multi-user RVM install with the latest ruby`
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\curl -sSL https://get.rvm.io | sudo bash -s stable --ruby
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source /etc/profile.d/rvm.sh
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# Add user to RVM group
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sudo usermod -a -G rvm vagrant
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su vagrant
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# Install transrate
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gem install transrate --pre
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# Install dependencies
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transrate --install-deps
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# -*- mode: ruby -*-
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# vi: set ft=ruby :
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# Vagrantfile API/syntax version. Don't touch unless you know what you're doing!
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VAGRANTFILE_API_VERSION = "2"
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Vagrant.configure(VAGRANTFILE_API_VERSION) do |config|
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config.vm.box = "andy_b_84/debian-squeeze64-vanilla"
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config.vm.network :forwarded_port, guest: 22, host: 18723
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end
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# -*- mode: ruby -*-
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# vi: set ft=ruby :
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# Vagrantfile API/syntax version. Don't touch unless you know what you're doing!
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VAGRANTFILE_API_VERSION = "2"
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Vagrant.configure(VAGRANTFILE_API_VERSION) do |config|
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# Every Vagrant virtual environment requires a box to build off of.
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config.vm.box = "http://files.dryga.com/boxes/osx-yosemite-0.2.0.box"
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# config.vm.synced_folder "../data", "/vagrant_data"
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config.vm.provider "virtualbox" do |vb|
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config.vm.synced_folder ".", "/vagrant", type: "nfs"
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end
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config.vm.provision "shell", path: "../../../packaging/build_deps_macosx.sh"
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end
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# -*- mode: ruby -*-
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# vi: set ft=ruby :
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# Vagrantfile API/syntax version. Don't touch unless you know what you're doing!
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VAGRANTFILE_API_VERSION = "2"
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Vagrant.configure(VAGRANTFILE_API_VERSION) do |config|
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# All Vagrant configuration is done here. The most common configuration
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# options are documented and commented below. For a complete reference,
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# please see the online documentation at vagrantup.com.
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# Every Vagrant virtual environment requires a box to build off of.
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config.vm.box = "hashicorp/precise64"
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# Provision the virtual machine with a local script, provision.sh,
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# in the same directory as the Vagrantfile
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config.vm.provision "shell", path: "provision.sh"
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# Disable automatic box update checking. If you disable this, then
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# boxes will only be checked for updates when the user runs
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# `vagrant box outdated`. This is not recommended.
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# config.vm.box_check_update = false
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# Create a forwarded port mapping which allows access to a specific port
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# within the machine from a port on the host machine. In the example below,
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# accessing "localhost:8080" will access port 80 on the guest machine.
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# config.vm.network "forwarded_port", guest: 80, host: 8080
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# Create a private network, which allows host-only access to the machine
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# using a specific IP.
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# config.vm.network "private_network", ip: "192.168.33.10"
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# Create a public network, which generally matched to bridged network.
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# Bridged networks make the machine appear as another physical device on
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# your network.
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# config.vm.network "public_network"
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# If true, then any SSH connections made will enable agent forwarding.
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# Default value: false
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# config.ssh.forward_agent = true
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# Share an additional folder to the guest VM. The first argument is
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# the path on the host to the actual folder. The second argument is
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# the path on the guest to mount the folder. And the optional third
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# argument is a set of non-required options.
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# config.vm.synced_folder "../data", "/vagrant_data"
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# Provider-specific configuration so you can fine-tune various
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# backing providers for Vagrant. These expose provider-specific options.
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# Example for VirtualBox:
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#
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# config.vm.provider "virtualbox" do |vb|
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# # Don't boot with headless mode
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# vb.gui = true
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#
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# # Use VBoxManage to customize the VM. For example to change memory:
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# vb.customize ["modifyvm", :id, "--memory", "1024"]
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# end
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#
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# View the documentation for the provider you're using for more
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# information on available options.
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# Enable provisioning with CFEngine. CFEngine Community packages are
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# automatically installed. For example, configure the host as a
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# policy server and optionally a policy file to run:
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#
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# config.vm.provision "cfengine" do |cf|
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# cf.am_policy_hub = true
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# # cf.run_file = "motd.cf"
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# end
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#
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# You can also configure and bootstrap a client to an existing
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# policy server:
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#
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# config.vm.provision "cfengine" do |cf|
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# cf.policy_server_address = "10.0.2.15"
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# end
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# Enable provisioning with Puppet stand alone. Puppet manifests
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# are contained in a directory path relative to this Vagrantfile.
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# You will need to create the manifests directory and a manifest in
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# the file default.pp in the manifests_path directory.
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#
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# config.vm.provision "puppet" do |puppet|
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# puppet.manifests_path = "manifests"
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# puppet.manifest_file = "site.pp"
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# end
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# Enable provisioning with chef solo, specifying a cookbooks path, roles
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# path, and data_bags path (all relative to this Vagrantfile), and adding
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# some recipes and/or roles.
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#
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# config.vm.provision "chef_solo" do |chef|
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# chef.cookbooks_path = "../my-recipes/cookbooks"
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# chef.roles_path = "../my-recipes/roles"
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# chef.data_bags_path = "../my-recipes/data_bags"
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# chef.add_recipe "mysql"
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# chef.add_role "web"
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#
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# # You may also specify custom JSON attributes:
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# chef.json = { mysql_password: "foo" }
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# end
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# Enable provisioning with chef server, specifying the chef server URL,
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# and the path to the validation key (relative to this Vagrantfile).
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#
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# The Opscode Platform uses HTTPS. Substitute your organization for
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# ORGNAME in the URL and validation key.
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#
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# If you have your own Chef Server, use the appropriate URL, which may be
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# validation key to validation.pem.
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#
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# chef.chef_server_url = "https://api.opscode.com/organizations/ORGNAME"
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# chef.validation_key_path = "ORGNAME-validator.pem"
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# end
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#
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# ORGNAME-validator, replacing ORGNAME with your organization name.
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#
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# chef-validator, unless you changed the configuration.
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# chef.validation_client_name = "ORGNAME-validator"
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end
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su vagrant
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# Setup
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sudo apt-get update -y
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sudo apt-get install -y curl build-essential git
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# Install a multi-user RVM install with the latest ruby
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sudo gpg --keyserver hkp://keys.gnupg.net --recv-keys D39DC0E3
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\curl -sSL https://get.rvm.io | bash -s stable --ruby
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source /usr/local/rvm/scripts/rvm
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# Add user to RVM group
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sudo usermod -a -G rvm vagrant
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# Install transrate
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git clone https://github.com/Blahah/transrate.git
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cd transrate
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sudo chmod -R 777 .
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# Install dependencies
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gem install bundler rake
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bundle install
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bundle exec rake compile
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bundle exec bin/transrate --install-deps
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data/transrate.gemspec
CHANGED
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gem.add_development_dependency 'test-unit', '~> 3.0'
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gem.add_development_dependency 'rake', '~> 10.3', '>= 10.3.2'
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gem.add_development_dependency 'rake-compiler', '~> 0.9', '>= 0.9.2'
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gem.add_development_dependency '
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gem.add_development_dependency 'minitest', '~> 5'
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gem.add_development_dependency 'minitest-reporters', '~> 1', '>= 1.0.17'
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gem.add_development_dependency 'simplecov', '~> 0.8', '>= 0.8.2'
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gem.add_development_dependency 'shoulda', '~> 3.5', '>= 3.5.0'
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gem.add_development_dependency 'coveralls', '~> 0.7', '>= 0.7.2'
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metadata
CHANGED
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--- !ruby/object:Gem::Specification
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name: transrate
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version: !ruby/object:Gem::Version
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version: 1.0.
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version: 1.0.1
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platform: ruby
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authors:
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- Richard Smith-Unna
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autorequire:
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bindir: bin
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cert_chain: []
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date: 2015-
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date: 2015-07-07 00:00:00.000000000 Z
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dependencies:
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name: yell
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requirement: !ruby/object:Gem::Requirement
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requirements:
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type: :development
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prerelease: false
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version_requirements: !ruby/object:Gem::Requirement
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requirements:
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requirements:
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requirements:
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extra_rdoc_files: []
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files:
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- CITATION
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- Gemfile
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- LICENSE
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- bin/transrate
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- docs/transrate_logo_full.png
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- ext/transrate/extconf.rb
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- files.txt
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- lib/transrate/cmd.rb
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- lib/transrate/cmdline.rb
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- lib/transrate/contig.rb
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- lib/transrate/transrater.rb
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- lib/transrate/writer.rb
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- packaging/build_deps_linux.sh
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- packaging/build_deps_macosx.sh
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- packaging/bundler-config
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- packaging/minify.sh
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|
+
- packaging/transrate
|
344
347
|
- test/data/150uncovered.l.fq
|
345
348
|
- test/data/150uncovered.r.fq
|
346
349
|
- test/data/Os.protein.2.fa
|
@@ -352,11 +355,20 @@ files:
|
|
352
355
|
- test/data/sorghum_100.fa
|
353
356
|
- test/data/sorghum_transcript.fa
|
354
357
|
- test/data/test.sf
|
358
|
+
- test/data/test_contig_nc1.fa
|
359
|
+
- test/data/test_contig_nc2.fa
|
360
|
+
- test/data/test_contig_nc3.fa
|
361
|
+
- test/data/test_contig_nc4.fa
|
362
|
+
- test/data/test_contig_nc5.fa
|
363
|
+
- test/data/test_contig_nc6.fa
|
364
|
+
- test/data/test_contig_nc7.fa
|
365
|
+
- test/data/test_reference_aa1.fa
|
366
|
+
- test/data/test_reference_nc1.fa
|
355
367
|
- test/data/tiny.sam
|
356
368
|
- test/helper.rb
|
357
369
|
- test/test_assembly.rb
|
358
|
-
- test/test_bin.rb
|
359
370
|
- test/test_cmd.rb
|
371
|
+
- test/test_cmdline.rb
|
360
372
|
- test/test_comp_metrics.rb
|
361
373
|
- test/test_contig.rb
|
362
374
|
- test/test_contig_metrics.rb
|
@@ -367,6 +379,15 @@ files:
|
|
367
379
|
- test/test_snap.rb
|
368
380
|
- test/test_transrate.rb
|
369
381
|
- test/test_transrater.rb
|
382
|
+
- test/vagrant/centos_6.5_64/Vagrantfile
|
383
|
+
- test/vagrant/debian_7.4_64/Vagrantfile
|
384
|
+
- test/vagrant/debian_7.4_64/provision.sh
|
385
|
+
- test/vagrant/fedora_20_64/Vagrantfile
|
386
|
+
- test/vagrant/fedora_20_64/provision.sh
|
387
|
+
- test/vagrant/linux-x86_64/Vagrantfile
|
388
|
+
- test/vagrant/osx/Vagrantfile
|
389
|
+
- test/vagrant/ubuntu_12.04_64/Vagrantfile
|
390
|
+
- test/vagrant/ubuntu_12.04_64/provision.sh
|
370
391
|
- transrate.gemspec
|
371
392
|
homepage: https://github.com/Blahah/transrate
|
372
393
|
licenses:
|
data/test/test_bin.rb
DELETED
@@ -1,139 +0,0 @@
|
|
1
|
-
require 'helper'
|
2
|
-
require 'csv'
|
3
|
-
|
4
|
-
class TestTransrateBin < Test::Unit::TestCase
|
5
|
-
|
6
|
-
context "Transrate" do
|
7
|
-
|
8
|
-
setup do
|
9
|
-
|
10
|
-
end
|
11
|
-
|
12
|
-
teardown do
|
13
|
-
files = ["sorghum_100.1.fastq.sorghum_100.2.fastq.sorghum_100.bam.bai",
|
14
|
-
"sorghum_100.1.fastq.sorghum_100.2.fastq.sorghum_100.bam",
|
15
|
-
"sorghum_100_into_Os.protein.2.1.blast",
|
16
|
-
"assembly.2_into_Os.protein.2.1.blast",
|
17
|
-
"sorghum_100.nhr", "sorghum_100.nin", "sorghum_100.nsq",
|
18
|
-
"Os.protein.2_into_sorghum_100.2.blast",
|
19
|
-
"Os.protein.2_into_assembly.2.2.blast",
|
20
|
-
"assembly.2.nhr", "assembly.2.nin", "assembly.2.nsq",
|
21
|
-
"Os.protein.2.phr", "Os.protein.2.pin", "Os.protein.2.psq",
|
22
|
-
"transrate_assemblies.csv",
|
23
|
-
"sorghum_100.fa_quant.sf",
|
24
|
-
"sorghum_100.fa_bam_info.csv",
|
25
|
-
"transrate_sorghum_100.fa_contigs.csv",
|
26
|
-
"sorghum_100.1.fastq-sorghum_100.2.fastq-read_count.txt",
|
27
|
-
"sorghum_100.1.fastq.sorghum_100.2.fastq.sorghum_100.assigned.bam",
|
28
|
-
"bad.sorghum_100.fa", "chimeric.sorghum_100.fa",
|
29
|
-
"fragmented.sorghum_100.fa", "good.sorghum_100.fa"]
|
30
|
-
files.each do |file|
|
31
|
-
File.delete(file) if File.exist?(file)
|
32
|
-
end
|
33
|
-
`rm -rf sorghum_100`
|
34
|
-
`rm -rf logs`
|
35
|
-
`rm -rf libParams`
|
36
|
-
end
|
37
|
-
|
38
|
-
should "run help" do
|
39
|
-
c=Transrate::Cmd.new("bundle exec bin/transrate --help")
|
40
|
-
c.run
|
41
|
-
assert c.stdout =~ /DESCRIPTION/
|
42
|
-
assert_equal true, c.status.success?, "exit status"
|
43
|
-
end
|
44
|
-
|
45
|
-
should "fail on non existent assembly files" do
|
46
|
-
c=Transrate::Cmd.new("bundle exec bin/transrate --assembly foo.fasta")
|
47
|
-
c.run
|
48
|
-
assert_equal false, c.status.success?, "exit success"
|
49
|
-
end
|
50
|
-
|
51
|
-
should "fail on non existent reference files" do
|
52
|
-
c=Transrate::Cmd.new("bundle exec bin/transrate --reference foo.fasta")
|
53
|
-
c.run
|
54
|
-
assert_equal false, c.status.success?, "exit status"
|
55
|
-
end
|
56
|
-
|
57
|
-
should "run on test data" do
|
58
|
-
assembly = File.join(File.dirname(__FILE__), 'data', 'sorghum_100.fa')
|
59
|
-
reference = File.join(File.dirname(__FILE__), 'data', 'Os.protein.2.fa')
|
60
|
-
left = File.join(File.dirname(__FILE__), 'data', 'sorghum_100.1.fastq')
|
61
|
-
right = File.join(File.dirname(__FILE__), 'data', 'sorghum_100.2.fastq')
|
62
|
-
cmd = "bundle exec bin/transrate --assembly #{assembly}"
|
63
|
-
cmd << " --reference #{reference}"
|
64
|
-
cmd << " --left #{left}"
|
65
|
-
cmd << " --right #{right}"
|
66
|
-
c = Transrate::Cmd.new("#{cmd}")
|
67
|
-
c.run
|
68
|
-
assert_equal true, c.status.success?, "exit status"
|
69
|
-
assert File.exist?("transrate_assemblies.csv"), "csv file doesn't exist"
|
70
|
-
assert File.exist?("transrate_sorghum_100.fa_contigs.csv"),
|
71
|
-
"contig csv file doesn't exist"
|
72
|
-
hash = {}
|
73
|
-
CSV.foreach("transrate_assemblies.csv", :headers => true,
|
74
|
-
:header_converters => :symbol,
|
75
|
-
:converters => :all) do |row|
|
76
|
-
row.headers
|
77
|
-
row.fields
|
78
|
-
row.headers.zip(row.fields).each do |header, field|
|
79
|
-
hash[header]=field
|
80
|
-
end
|
81
|
-
end
|
82
|
-
assert_in_delta 137748, hash[:n_bases], 1000, "number of bases"
|
83
|
-
assert_equal 1692, hash[:n50], "n50"
|
84
|
-
assert_equal 2, hash[:n_refs_with_crbb], "number of crb hits"
|
85
|
-
assert_equal 2, hash[:n_contigs_with_crbb], "number of contigs with hits"
|
86
|
-
end
|
87
|
-
|
88
|
-
should "run on test data with comma separated list of fastq files" do
|
89
|
-
assembly = File.join(File.dirname(__FILE__), 'data', 'sorghum_100.fa')
|
90
|
-
left = []
|
91
|
-
right = []
|
92
|
-
left << File.join(File.dirname(__FILE__), 'data', 'sorghum_100.1.fastq')
|
93
|
-
left << File.join(File.dirname(__FILE__), 'data', '150uncovered.l.fq')
|
94
|
-
right << File.join(File.dirname(__FILE__), 'data', 'sorghum_100.2.fastq')
|
95
|
-
right << File.join(File.dirname(__FILE__), 'data', '150uncovered.r.fq')
|
96
|
-
cmd = "bundle exec bin/transrate --assembly #{assembly}"
|
97
|
-
cmd << " --left #{left.join(",")}"
|
98
|
-
cmd << " --right #{right.join(",")}"
|
99
|
-
c = Transrate::Cmd.new("#{cmd}")
|
100
|
-
c.run
|
101
|
-
if !(c.status.success?)
|
102
|
-
puts c.stdout
|
103
|
-
puts c.stderr
|
104
|
-
end
|
105
|
-
assert_equal true, c.status.success?, "exit status"
|
106
|
-
assert File.exist?("transrate_assemblies.csv")
|
107
|
-
assert File.exist?("transrate_sorghum_100.fa_contigs.csv"),
|
108
|
-
"contig csv file doesn't exist"
|
109
|
-
hash = {}
|
110
|
-
CSV.foreach("transrate_assemblies.csv", :headers => true,
|
111
|
-
:header_converters => :symbol,
|
112
|
-
:converters => :all) do |row|
|
113
|
-
row.headers.zip(row.fields).each do |header, field|
|
114
|
-
hash[header]=field
|
115
|
-
end
|
116
|
-
end
|
117
|
-
assert_in_delta 137748, hash[:n_bases], 1000, "number of bases"
|
118
|
-
assert_equal 1692, hash[:n50], "n50"
|
119
|
-
assert_equal 25006 + 223, hash[:fragments], "number of reads"
|
120
|
-
end
|
121
|
-
|
122
|
-
should "fail when one of multiple assemblies is missing" do
|
123
|
-
assembly = File.join(File.dirname(__FILE__), 'data', 'assembly.2.fa')
|
124
|
-
assembly2 = File.join(File.dirname(__FILE__), 'data', 'foo.fa')
|
125
|
-
reference = File.join(File.dirname(__FILE__), 'data', 'Os.protein.2.fa')
|
126
|
-
left = File.join(File.dirname(__FILE__), 'data', '150uncovered.l.fq')
|
127
|
-
right = File.join(File.dirname(__FILE__), 'data', '150uncovered.r.fq')
|
128
|
-
cmd = "bundle exec bin/transrate "
|
129
|
-
cmd << " --assembly #{assembly},#{assembly2}"
|
130
|
-
cmd << " --reference #{reference}"
|
131
|
-
cmd << " --left #{left}"
|
132
|
-
cmd << " --right #{right}"
|
133
|
-
c = Transrate::Cmd.new("#{cmd}")
|
134
|
-
c.run
|
135
|
-
assert_equal false, c.status.success?, "exit status"
|
136
|
-
end
|
137
|
-
|
138
|
-
end
|
139
|
-
end
|