transrate 0.1.0 → 0.2.0

data/lib/transrate/contig_metrics.rb

@@ -0,0 +1,76 @@
+require 'set'
+require 'inline'
+
+module Transrate
+
+  class ContigMetrics
+
+    attr_reader :gc_prop, :gc_skew, :at_skew, :cpg_ratio
+    attr_reader :linguistic_complexity, :wootton_federhen, :shannon, :zlib_comp
+    attr_reader :bases_n, :proportion_n
+    attr_reader :has_run
+
+    def initialize assembly
+      @assembly = assembly
+      self.initial_values
+      @has_run = false
+    end
+
+    def initial_values
+      @gc_prop = -1
+      @gc_skew = -1
+      @at_skew = -1
+      @cpg = -1
+      @bases_n = -1
+      @proportion_n = -1
+      @linguistic_complexity = -1
+    end
+
+    def run
+      calculate
+      @has_run = true
+    end
+
+    def calculate
+      total = 0
+      a = 0
+      c = 0
+      g = 0
+      t = 0
+      @bases_n = 0
+      cpg_count = 0
+      lc = 0
+      k = 6
+      @assembly.assembly.each do |contig|
+        total += contig.length
+        a += contig.bases_a
+        c += contig.bases_c
+        g += contig.bases_g
+        t += contig.bases_t
+        @bases_n += contig.bases_n
+        cpg_count += contig.cpg_count
+        lc += contig.linguistic_complexity k
+      end
+      @gc_prop = (g + c) / (a + c + g + t).to_f
+      @gc_skew = (g - c) / (g + c).to_f
+      @at_skew = (a - t) / (a + t).to_f
+      @cpg_ratio = cpg_count.to_f / (c * g) * total
+      @linguistic_complexity = lc / @assembly.assembly.size.to_f
+      @proportion_n = @bases_n / total.to_f
+    end
+
+    def results
+      return if !@has_run
+      return {'gc' => @gc_prop,
+              'gc_skew' => @gc_skew,
+              'at_skew' => @at_skew,
+              'cpg_ratio' => @cpg_ratio,
+              'bases_n' => @bases_n,
+              'proportion_n' => @proportion_n,
+              'linguistic_complexity' => @linguistic_complexity
+             }
+    end
+
+  end
+
+end

data/lib/transrate/read_metrics.rb

@@ -2,6 +2,9 @@ module Transrate
 
   class ReadMetrics
 
+    require 'bettersam'
+    require 'bio-samtools'
+
     attr_reader :total
     attr_reader :bad
     attr_reader :supported_bridges
@@ -9,18 +12,28 @@ module Transrate
     attr_reader :percent_mapping
     attr_reader :prop_expressed
     attr_reader :has_run
-    
+
     def initialize assembly
       @assembly = assembly
       @mapper = Bowtie2.new
       self.initial_values
     end
 
-    def run left, right, insertsize=200, insertsd=50
+    def run left, right, insertsize:200, insertsd:50, threads:8
+      [left, right].each do |readfile|
+        unless File.exist? readfile
+          raise IOError.new "ReadMetrics read file does not exist: #{readfile}"
+        end
+      end
       @mapper.build_index @assembly.file
-      samfile = @mapper.map_reads(@assembly.file, left, right,  insertsize, insertsd)
-      self.analyse_read_mappings(samfile, insertsize, insertsd)
-      self.analyse_expression(samfile)
+      @num_pairs = `wc -l #{left}`.strip.split(/\s+/)[0].to_i/4
+      samfile = @mapper.map_reads(@assembly.file, left, right,
+                                  insertsize: insertsize,
+                                  insertsd: insertsd,
+                                  threads: threads)
+      # check_bridges
+      analyse_read_mappings(samfile, insertsize, insertsd, true)
+      analyse_coverage(samfile)
       @pr_good_mapping = @good.to_f / @num_pairs.to_f
       @percent_mapping = @total.to_f / @num_pairs.to_f * 100.0
       @pc_good_mapping = @pr_good_mapping * 100.0
@@ -35,20 +48,16 @@ module Transrate
         :good_mappings => @good,
         :pc_good_mapping => @pc_good_mapping,
         :bad_mappings => @bad,
-        :both_mapped => @both_mapped,
-        :properly_paired => @properly_paired,
-        :improper_paired => @improperly_paired,
-        :proper_orientation => @proper_orientation,
-        :improper_orientation => @improper_orientation,
-        :same_contig => @same_contig,
-        :realistic_overlap => @realistic_overlap,
-        :unrealistic_overlap => @unrealistic_overlap,
-        :realistic_fragment => @realistic_fragment,
-        :unrealistic_fragment => @unrealistic_fragment,
         :potential_bridges => @supported_bridges,
-        :expressed_contigs => @expressed_contigs,
-        :unexpressed_contigs => @unexpressed_contigs,
-        :percent_expressed => @percent_expressed
+        :mean_coverage => @mean_coverage,
+        :n_uncovered_bases => @n_uncovered_bases,
+        :p_uncovered_bases => @p_uncovered_bases,
+        :n_uncovered_base_contigs => @n_uncovered_base_contigs,
+        :p_uncovered_base_contigs => @p_uncovered_base_contigs,
+        :n_uncovered_contigs => @n_uncovered_contigs,
+        :p_uncovered_contigs => @p_uncovered_contigs,
+        :n_lowcovered_contigs => @n_lowcovered_contigs,
+        :p_lowcovered_contigs => @p_lowcovered_contigs
       }
     end
 
@@ -58,20 +67,33 @@ module Transrate
       if File.exists?(samfile) && File.size(samfile) > 0
         ls = BetterSam.new
         rs = BetterSam.new
-        sam = File.open(samfile).each_line
-        sam.each_slice(2) do |l, r|
-          if (l && r) && (ls.parse_line(l) && rs.parse_line(r))
-            self.check_read_pair(ls, rs, realistic_dist)
+        sam = File.open(samfile)
+        line = sam.readline
+        while line and line=~/^@/
+          line = sam.readline rescue nil
+        end
+        while line
+          ls.parse_line(line)
+          if ls.mate_unmapped?
+            self.check_read_single(ls)
+            line = sam.readline rescue nil
+          else
+            line2 = sam.readline rescue nil
+            if line2
+              rs.parse_line(line2)
+              self.check_read_pair(ls, rs, realistic_dist)
+            end
+            line = sam.readline rescue nil
           end
         end
-        self.check_bridges if bridge
+        check_bridges
       else
         raise "samfile #{samfile} not found"
       end
     end
 
     def initial_values
-      @num_pairs = 100000
+      @num_pairs = 0
       @total = 0
       @good = 0
       @bad = 0
@@ -85,20 +107,26 @@ module Transrate
       @unrealistic_overlap = 0
       @realistic_fragment = 0
       @unrealistic_fragment = 0
-      @unexpressed_contigs = 0
-      @expressed_contigs = 0
+      @n_uncovered_bases = 0
+      @n_uncovered_base_contigs = 0 # any base cov < 1
+      @n_uncovered_contigs = 0 # mean cov < 1
+      @n_lowcovered_contigs = 0 # mean cov < 10
     end
 
     def realistic_distance insertsize, insertsd
       insertsize + (3 * insertsd)
     end
 
+    def check_read_single ls
+
+    end
+
     def check_read_pair ls, rs, realistic_dist
       return unless ls.primary_aln?
       @total += 1
       if ls.both_mapped?
         # reads are paired
-        @both_mapped += 1
+        @both_mapped += 1 if ls.primary_aln?
         if ls.read_properly_paired?
           # mapped in proper pair
           @properly_paired += 1
@@ -176,21 +204,35 @@ module Transrate
       end
     end
 
-    def analyse_expression samfile
-      express = Express.new
-      @expression = express.quantify_expression(@assembly.file, samfile)
-      @expression.each_pair do |target, count|
-        count = count.to_f
-        if count == 0
-          @unexpressed_contigs += 1
-        elsif count > 0
-          @expressed_contigs += 1
-        end
+    # Generate per-base and contig read coverage statistics.
+    # Note that contigs less than 200 bases long are ignored in this
+    # analysis.
+    def analyse_coverage samfile
+      bamfile, sorted, index = Samtools.sam_to_sorted_indexed_bam samfile
+      bam = Bio::DB::Sam.new(:bam => sorted, :fasta => @assembly.file)
+      # get per-base coverage and calculate mean,
+      # identify zero-coverage bases
+      n, tot_length, tot_coverage = 0, 0, 0
+      @assembly.each_with_coverage(bam) do |contig, coverage|
+        next if contig.length < 200
+        zerocov, total = 0, 0
+        coverage.each { |e| total += e; zerocov += 1 if e < 1 }
+        tot_length += coverage.length
+        tot_coverage += total
+        mean = total / coverage.length.to_f
+        @n_uncovered_bases += zerocov
+        @n_uncovered_base_contigs += 1 if zerocov > 0
+        @n_uncovered_contigs += 1 if mean < 1
+        @n_lowcovered_contigs += 1 if mean < 10
       end
-      @prop_expressed = @expressed_contigs.to_f / @assembly.size
-      @percent_expressed = @prop_expressed * 100.0
+      @mean_coverage = (tot_coverage / tot_length.to_f).round(2)
+      @p_uncovered_bases = @n_uncovered_bases / @assembly.n_bases.to_f
+      @p_uncovered_base_contigs = @n_uncovered_base_contigs /
+                                  @assembly.size.to_f
+      @p_uncovered_contigs = @n_uncovered_contigs / @assembly.size.to_f
+      @p_lowcovered_contigs = @n_lowcovered_contigs / @assembly.size.to_f
     end
-    
+
   end # ReadMetrics
-  
+
 end # Transrate

data/lib/transrate/samtools.rb

@@ -0,0 +1,73 @@
+require 'bio-samtools'
+
+module Transrate
+
+  class Samtools
+
+    # Get the path to the samtools binary built when bio-samtools
+    # was installed
+    def self.path
+      gem_path = Gem.loaded_specs['bio-samtools'].full_gem_path
+      return File.join(gem_path, 'lib/bio/db/sam/external/samtools')
+    end
+
+    # Run a samtools command
+    def self.run cmd
+      runcmd = Cmd.new "#{Samtools.path} #{cmd}"
+      runcmd.run
+      runcmd.stdout
+    end
+
+    # Convert a sam file to a bam file, returning the path to the bamfile
+    def self.sam_to_bam samfile
+      bamfile = File.basename(samfile, '.sam') + '.bam'
+      bamfile = File.expand_path bamfile
+      Samtools.run "view -bS #{File.expand_path samfile} > #{bamfile}"
+      File.expand_path bamfile
+    end
+
+    # Sort a bam file, returning the path to the sorted bamfile
+    def self.sort_bam bamfile
+      # the sort command behaves inconsistently with the other commands:
+      # it takes an output prefix rather than a filename
+      # and automatically adds the .bam extension
+      sorted = File.basename(bamfile, '.bam') + '.sorted'
+      Samtools.run "sort #{File.expand_path bamfile} #{sorted}"
+      File.expand_path(sorted + '.bam')
+    end
+
+    # Index a bamfile, returning the path to the index
+    def self.index_bam bamfile
+      index = File.basename(bamfile, '.bam') + '.bai'
+      index = File.expand_path index
+      Samtools.run "index #{File.expand_path bamfile} #{index}"
+      index
+    end
+
+    # Convert a sam file to bam, sort and index the bam, returning
+    # an array of paths to the bamfile, sorted bamfile and index respectively
+    def self.sam_to_sorted_indexed_bam samfile
+      bamfile = Samtools.sam_to_bam samfile
+      sorted = Samtools.sort_bam bamfile
+      index = Samtools.index_bam bamfile
+      [bamfile, sorted, index]
+    end
+
+    # Calculate per-base coverage from a sorted, indexed bam file
+    # return the path to the coverage file
+    def self.coverage bam
+      outfile = File.expand_path "#{File.basename(bam.fasta)}.coverage"
+      cmd = "mpileup"
+      cmd += " -f #{File.expand_path bam.fasta}" # reference
+      cmd += " -B" # don't calculate BAQ quality scores
+      cmd += " -Q0" # include all reads ignoring quality
+      cmd += " -I" # don't do genotype calculations
+      cmd += " #{File.expand_path bam.bam}" # the bam file
+      cmd += " > #{outfile}"
+      Samtools.run cmd
+      outfile
+    end
+
+  end
+
+end

data/lib/transrate/transrater.rb

@@ -22,13 +22,32 @@ module Transrate
     # @param right [String] path to the right reads
     # @param insertsize [Integer] mean insert size of the read pairs
     # @param insertsd [Integer] standard deviation of the read pair insert size
-    def initialize assembly, reference, left=nil, right=nil, insertsize=nil, insertsd=nil
-      @assembly  = assembly.is_a?(Assembly)  ? assembly  : Assembly.new(assembly)
+    def initialize(assembly, reference,
+                   left: nil, right: nil,
+                   insertsize: nil, insertsd: nil,
+                   threads: 1)
+      if assembly
+        if assembly.is_a?(Assembly)
+          @assembly = assembly
+        else
+          @assembly = Assembly.new(assembly)
+        end
+        @read_metrics = ReadMetrics.new @assembly
+      else
+        raise RuntimeError.new("assembly is nil")
+      end
+
       if reference
-        @reference = reference.is_a?(Assembly) ? reference : Assembly.new(reference)
+        if reference.is_a?(Assembly)
+          @reference = reference
+        else
+          @reference = Assembly.new(reference)
+        end
+        @comparative_metrics = ComparativeMetrics.new(@assembly,
+                                                      @reference,
+                                                      threads)
       end
-      @read_metrics = ReadMetrics.new @assembly
-      @comparative_metrics = ComparativeMetrics.new(@assembly, @reference)
+      @threads = threads
     end
 
     # Run all analyses
@@ -47,7 +66,7 @@ module Transrate
 
     # Reduce all metrics for the assembly to a single quality score.
     #
-    # 
+    #
     #
     # @return [Integer] the assembly score
     def assembly_score
@@ -56,8 +75,7 @@ module Transrate
         pg = Metric.new('pg', @read_metrics.pr_good_mapping, 0.0)
       end
       if @comparative_metrics.has_run
-        rc = Metric.new('rc', @comparative_metrics.reference_coverage,
-                    0.0)
+        rc = Metric.new('rc', @comparative_metrics.reference_coverage, 0.0)
       end
       if (pg && rc)
         @score = DimensionReduce.dimension_reduce([pg, rc])
@@ -71,12 +89,14 @@ module Transrate
     end
 
     def read_metrics left=nil, right=nil
-      @read_metrics.run(left, right) unless @read_metrics.has_run
+      unless @read_metrics.has_run
+        @read_metrics.run(left, right, threads: @threads)
+      end
       @read_metrics
     end
 
     def comparative_metrics
-      @comparative_metrics.run #unless @comparative_metrics.has_run
+      @comparative_metrics.run unless @comparative_metrics.has_run
       @comparative_metrics
     end
 
@@ -88,7 +108,7 @@ module Transrate
       all[:score] = @score
       all
     end
-    
+
   end # Transrater
 
 end # Transrate

data/lib/transrate/version.rb

@@ -9,7 +9,7 @@ module Transrate
   # Semantic Versioning 2.0 (http://semver.org/).
   module VERSION
     MAJOR = 0
-    MINOR = 1
+    MINOR = 2
     PATCH = 0
     BUILD = nil
 

data/test/data/150uncovered.l.fq

@@ -0,0 +1,892 @@
+@FCC2HFRACXX:7:1101:2015:1981#TGACCAAT/1
+GTCGAGGCCGTCAGGCATGTGCGGTCCGTCATGGGCGATGTCCGTGCGCTCCGGAACATGGATGATGATGAGGTGTTCGCGTATGCTAAGCGCATCGCCG
++
+_[_eeeeegebe`efddgaeehhhhfgfQ^eb`adfgfffhZ_db\adb`aaabaWQT]`b_b]Y_b`cbbb`]`][]^]aaX^bbdbbY^[_a_[[O][
+@FCC2HFRACXX:7:1101:7968:3054#TGACCAAT/1
+GGCCACGCCTGCTGATGCCGCGCTCATGATGCAGCTTGGGTGCGACGGCGTCTTCGTCGGCTC
++
+bbbeeeeef`gcghcegdghfhfdfdfdghhfgfhhhhhgG_Z`__`c[_RZa^baX^aaZ_a
+@FCC2HFRACXX:7:1101:18523:3712#TGACCAAT/1
+CTCATGTCTCTCCATCAGTAATAACGGGACTGAATCAATGGTAAGAAACAAAGCTATGGTACTATGCAGAACCTCTTTATTTTT
++
+__^cc`\cecabcdea_d[`b^[abaUeW^a_e[S^afS^^Y^acbfedeb]^cacefh_V\W\efbddYcd`deV\aRV^ccc
+@FCC2HFRACXX:7:1101:6215:4551#TGACCAAT/1
+TATGATTTGGTGATGCAGACCAAGCAGCTGGGCCGCCTCCCTGTTGTGCAGTTCGCGGCCGGGGGTGTGGCCACGCCTGCTGATGCCGCGCTCATGATGC
++
+__[cccceggbeeddghhdhhhhhdhhfbfhhhhhhhhhhhhhghfefg_fdcgbdaabaaa_[]LEO^^^``[X_^^`ab`]]_bbac_X]XEYR_b]_
+@FCC2HFRACXX:7:1101:9701:4567#TGACCAAT/1
+GTTCAGGCTGTCACCCACTACAGCGACCCTACCATCCTCGCCGACGTCAGCGCCGGACTCGGGGAGGCCATGGTCGGCATCAACCTCAACGACCCTA
++
+b_beeeeeggggfiihhihihifhiiiiiiihfhefhiihiiiiigeedcdcca^cacccacccccc_acbbbS]][_[ab_`X`]`bcc[[X_a^b
+@FCC2HFRACXX:7:1101:20111:6108#TGACCAAT/1
+GTAAGAAACAAAGCTATGGTACTATGCAGAACCTCTTTATTTTTGTGTCTTCTTATTAGCTACTGTATTAAGTTAGACTAGGCTCCCCTGGTTTATCATG
++
+_bbeceeegggegihhiiidghhiiffhhfhhfhhihighhiihhfgfhhiffhhihdfegbgifbbfghf]bdffbRcgddgfdeee]^^GZ^]_]bbZ
+@FCC2HFRACXX:7:1101:2825:6457#TGACCAAT/1
+CGCTTTGGCCATGACGGGGATGGTGACGGCGCGCTTGATGTCGCGGATGAGGCCCGGGTCGGACATCCGCGCCACGCCGCCCTGCGAGCGGATGTCGGCG
++
+bbbeeeeeggfggiiiiiigffhbfgiiihfhdaggdgeeeeaecaaccc_b[^aUYXT[V[ZaV_`bL[XZ_TXX]a[[aaOW`a]_]OXXXa]]^[X_
+@FCC2HFRACXX:7:1101:7858:6597#TGACCAAT/1
+GGGGGAGCCTAGTCTAACTTAATACAGTAGCTAATAAGAAGACACAAAAATAAAGAGGTTCTGCATAGTACCATAGCTTTGTTTCTTACCATTGATTCAG
++
+bbbeeeeegfggeiiiiiiihifhhhhhiiiiihhhihhhhhhihhhhhiiiih_efg`fghhffhhggfgggfeceeeeddddddcccbcccccdcdcb
+@FCC2HFRACXX:7:1101:5948:9388#TGACCAAT/1
+CCGTTATTACTGATGGAGAGACATGAGACAAAATAAGCTACTGTTTGTAATGCAAAAATTATTAAGAAGAGAAGGATGAAGCCGGCGGAGTGGAGATGCA
++
+_b_eeeceefgeafdhifffbbggdbgh_cfgfgha_]eghfchhffhdefebffhghh[efffffb`b]dd`dgdgR^ccYZ]`a\XXa^GOYQXRS]`
+@FCC2HFRACXX:7:1101:1327:10285#TGACCAAT/1
+AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
++
+bbbeeeeegggggihicghihiiiiihaaghegaeehhgecaaaacc_cbaaaaU_cb`b[acaccOTVZETZ
+@FCC2HFRACXX:7:1101:8651:10922#TGACCAAT/1
+CCTCAACGACCCTAAGGTCGAGCGATACGCCGCCAGATCCGAGTGAACAACCCTATCCGCTCCACCATCGTCATCTTCTCTCATATATTTGTATCACCCA
++
+bbbeeeeegggfgiiiibghfhcgffhfhiiiiiiiagggdeeccdddddccccbcbc^accccbaccbcW^[``S]_bbccccb_cdcdccccbcbccc
+@FCC2HFRACXX:7:1101:20856:13766#TGACCAAT/1
+CATTCATAAGCCATAAGCAGGGCTGGCAGAGCGACAGACATACAAACCGTTCAACATTTGATTGAGTACATGATAAACCAGGGGAGCCTAGTCTAACTTA
++
+bbbeeeeeggggfiiiiigfihffhhfghfhhhhffiffhibefhhhfg_cccdd_]`g[^dcebb]__dZYUZ__`bbca_^YRKT[]_bY]bbSY]bS
+@FCC2HFRACXX:7:1101:1878:16590#TGACCAAT/1
+CTGCATAGTACCATAGCTTTGTTTCTTACCATTGATTCAGTCCCGTTATTACTGATGGAGAGACATGAGACAAAATAAGCTACTGTTTGTAATGCAAAAA
++
+__bcceeeegfgggef_fg[hddgbfhghfffgfdhhhhhfg[_efghhhhhhhdfg[cfgch]ffhfbfg_fgf^dgeggeeeeceddd_`]Z`_Z]TZ
+@FCC2HFRACXX:7:1101:14970:16874#TGACCAAT/1
+ATTCAGTCCCGTTATTACTGATGGAGAGACATGAGACAAAATAAGCTACTGTTTGTAATGCAAAAATTATTAAGAAGAGAAGGATGAAGCCGGCGGAGTG
++
+_bbeeeeeggfggdgiiiiidgfhfhfdggfhhhhehhffihihfiidgfhcghhghihhihhiifhghh_cdgdb`]_ac]ca_bbbdc__aaca_aOW
+@FCC2HFRACXX:7:1101:19160:19344#TGACCAAT/1
+CCGCGCGCGTGCCATCGTTCAGGCTGTCACCCACTACAGCGACCCTACCATCCTCGCCGACGTCAGCGCCGGACT
++
+___eeeeegcccghfhhhhcbfffhdfe`__cffchhhb]``g[^bddbbbb^`bU^^\Zaaaaac_Zac]ZETO
+@FCC2HFRACXX:7:1101:20663:24038#TGACCAAT/1
+GGGGAGGCCATGGTCGGCATCAACCTCAACGACCCTAAGGTCGAGCGATACGCCGCCAGATCCGAG
++
+__bccccaegggeghiaeagfhchhhfhiXcaefffb_eg\_fhfaadeccc_c^ccca^YJY^aa
+@FCC2HFRACXX:7:1101:8756:24567#TGACCAAT/1
+CCATGATCCGCACCAAGGGGGAGGCCGGGACCGGCAACATCGTCGAGGCCGTCAGGCATGTGCGGTCCGTCATGGGCGATGTCCGTGCGCTCCGG
++
+bbbeeeeefgefghiihiiiiihifiiiiifdfgeaccdccc`acaaccca[a_ac^WY^Y_bccTT]a[`O[YYW`a]aaa]_WW[X_]_ac[a
+@FCC2HFRACXX:7:1101:15803:25761#TGACCAAT/1
+ATCGTCATCTTCTCTCATATATTTGTATCACCCATCCATCCATCTGCCTTCGATATGCATCTCCACTCCGCCGGCTTCATCCTTCTCTTCTTAATAATTT
++
+bbbcceeegffgghfhfhhehhhi]ghhhhihihf_e`ghfhdgfdgfhhhighhfhihhfhifffdXedffd[NRZ^]bb]_bbbccbcbcRR]_RSYb
+@FCC2HFRACXX:7:1101:5766:26080#TGACCAAT/1
+TGGAGATGCATATCGAAGGCAGATGGATGGATGGGTGATACAAATATATGAGAGAAGATGACGATGGTGGAGCGGATAGGGTTGTTCACTCGGATC
++
+___ccaccggeggfhfdfhded`aggZ[ef]_eghbce_eggcfhdcdfggf``eefdfff__b`cg\^dZZbbcc_c`baYX\F[RRY_`[^[a_
+@FCC2HFRACXX:7:1101:7987:27278#TGACCAAT/1
+GTTCACTCGGATCTGGCGGCGTATCGCTCGACCTTAGGGTCGTTGAGGTTGATGCCGACCATGGCCTCCCCGAGTCCGGCGCTGACGTCGGCGAGGATGG
++
+babeeeeegggggihhhiihicffhifhicdfeggeeee^b`X_^accY[[^bR]baOZacccb`b_`acaTTX[bcaaaaXRX[ba^aaVOXX_aER]_
+@FCC2HFRACXX:7:1101:17662:30576#TGACCAAT/1
+ATTGATTCAGTCCCGTTATTACTGATGGAGAGACATGAGACAAAATAAGCTACTGTTTGTAATGCAAAAATTATTAAGAAGAGAAGGATGAAGCCGGCG
++
+___eeeeegceegihhhffhehhigdfhhihifffghhhfhhiefhhagddcfbgaaefbegdffhihfhiefeeddgcdeeeeedddbcbbbbaacac
+@FCC2HFRACXX:7:1101:4976:31550#TGACCAAT/1
+CTGCGCCTCGACGAAGTGGCCGATGCGCGCTTTGGCCATGACGGGGATGGTGACGGCGCGCTTGATGTCGCGGGTG
++
+_bbeeeeeggg`ehfge[dfhhfffgfcdaNZdfgdhddg_gcebaRZ[[TTY__aWaLVZLZaQ]]SY``acEOT
+@FCC2HFRACXX:7:1101:8850:31628#TGACCAAT/1
+CTGCATCATGAGCGCGGCATCAGCAGGCGTGGCCACACCCCCGGCCGCGAA
++
+bb_eeeeeggggghhhhiiiiiiiiiiff`egffgihiihggX]aaW\WEV
+@FCC2HFRACXX:7:1101:6311:33629#TGACCAAT/1
+GTCTGCATCACCAAATCATACGGCGCGGCGATGCGCTTAGCATACGCGAACACCTCATCATCATCCATGTTCCGGAGCGCACGGACATCGCCCATGACGG
++
+_bbecacecgg`ghhfhhfghihgghhhffdgggeebcc`bbccccbaccccRQ]^]b`bbcbY`bRS]`bbbccaaccaaccccccbb_aacabbbb[a
+@FCC2HFRACXX:7:1101:16617:35398#TGACCAAT/1
+GAGCGGATAGGGTTGTTCACTCGGATCTGGCGGCGTATCGCTCGACCTTAGGGTCGTTGAGGTTGATG
++
+aabeeecegggg`fgdgfhihiiighhihhhiidgacc`bc`acN^a\b]`bcR]aTY^[YbRQRGSY
+@FCC2HFRACXX:7:1101:5129:41564#TGACCAAT/1
+GGGCGATGTCCGTGCGCTCCGGAACATGGATGATGATGAGGTGTTCGCGTATGCTAAGCGCATCGCCGCGCCGTATGATTTGGTGATGCAGACCAAGCAG
++
+bbbeeeeeggggeh`fhhiiiiiii_fgicfhhiiiiihgi_egfghiegecddddcdccccca_ccccccac]a^b`ccbbb^_bcbcccccabccccb
+@FCC2HFRACXX:7:1101:10393:43005#TGACCAAT/1
+CGCCGATCCATTCCATTCCATTCCAACTCAATCCCCCCCCCACCCAGCCCAAACAATGGCGTCGGACGGGAGCGGCGTGGTGACGGGGTACGGGAACAAC
++
+bbbeeeeeggggeiiiiihiiihhihdgihiifdgfhhihgfeeec^`bacccccbbbbccZ_a]_aaccaacccacLX[GWSY^aLXOT]T]aOTWW`W
+@FCC2HFRACXX:7:1101:10247:49111#TGACCAAT/1
+AGTCGGACGGGAGCGGCGTGGTGACGGTGTACGGGAACAACGGCGCAGCCCTTCTGGAACCCTCAAAGCAGCCCAAGTCGGCGACTTTCTCC
++
+_b_eeeeegggcgiiihiehiQ_bfgi\d`bbcecbcccccacaacacacc^bbc`b_]b^a`c[Y__bbbW^aQW[]bacca]a[^b_b`b
+@FCC2HFRACXX:7:1101:16334:49689#TGACCAAT/1
+CCGGTGGAGTGGAGATGCATATCGAAGGCAGATGGATGGATGGGTGATACAAATATATGAGAGAAGATGACGATGGTGGAGCGGATAGGGT
++
+_abeccecgbcgehififhihiiiihihhfhghfhffhhgdfgiX__edgfffffhfhiffghhe``Y^bdea\``GZ^\`\\_YLTX```
+@FCC2HFRACXX:7:1101:4903:52289#TGACCAAT/1
+GGGCGATGTCCGTGCGCTCCGGAACATGGATGATGATGAGGTGTTCGCGTATGCTAAGCGCATCGCCGCGCCGTATGATTTGGTGATGCAGACCAAGCAG
++
+^__eecceggggghhifiiihiffhghhifhefghhihhhibghhiiheddeccdcddbcaaac_accccccc]aab`cdccbaa_bccccbcccccccc
+@FCC2HFRACXX:7:1101:7290:52681#TGACCAAT/1
+TCCTGTTATTACTGATGGAGAGACATGAGACAAAATAAGCTACTGTTTGTAATGCAAAAATTATTAAGAAGAGAAGGATGAAGCCGGCGGAGTGGAGATG
++
+bbbeeeeegggggifiiiiiiiiiiiiiiiiiiiiiiiihiiiiighiigiiiiihhhiiihiiihhiffhhhhhcgegfeeeeeccccc_a_aa`a`bb
+@FCC2HFRACXX:7:1101:15630:52667#TGACCAAT/1
+CCACGGACCCTCCGGAGCGCCTCACCAAGGTCACGGCAGCCACAGACGAAGGGGACACGGAAGTTGTGCTTGTTGATGTGG
++
+bbbeeeeeggegghghiefcgfhiifiifg^egb`g_dgeefgeeee^X]TW]aFR^`acLWVY]_GQSY]_W_[GY_cbb
+@FCC2HFRACXX:7:1101:2561:53863#TGACCAAT/1
+CTAGTCTAACTTAATACAGTAGCTAATAAGAAGACACAAAAATAAAGAGGTTCTGCATAGTACCATAGCTTTGTTTCTTACCATTGATTCAGTCCCGTTA
++
+bbbeeeeegggggiiihiiffhhhhhhihhihhiihihiifbghhhdhihafggifghifhgfhiihihhdghcfbfghggffggeeedceedddbcac^
+@FCC2HFRACXX:7:1101:3406:56187#TGACCAAT/1
+CGCTCCGGAGGGTCCGTGAGGGCGCCGCCATGATCCGCACCAAGGGGGAGGCCGGGACCGGCAACATCGTCGAGGCCGTCAGGCATGTGCGGTCC
++
+___ccccc^cgcJ`bffdhffghdfhfhhdccbggeggU^b``aaaaa\Waaaaaaaa[aaaaaaa[]W[_ZXZ[[_TOX_abb[]a_]_O[T[a
+@FCC2HFRACXX:7:1101:1778:57411#TGACCAAT/1
+CAACTTCCGTGTCCCCTTCGTCTGTGGCTGCCGTGACCTTGGTGAGGCGCTCCGGAGGGTCCGTGAGGGCG
++
+_bbeec^cgegeghhfhhg_eedg_affbd\edd]Pa]ehdfaPcZaU`UN\`W^[X^WTY^aTWT[^^LT
+@FCC2HFRACXX:7:1101:7177:57638#TGACCAAT/1
+CGCCGGACTCGGGGAGGCCATGGTCGGCATCAACCTCAACGACCCTAAGGTCGAGCGATACACCGCCAGATCCGAGTGAACAACCCTATCCGCTCCACCA
++
+bbbeeeeefggggihhihiiiiifhfhihiiiiiiiiggggeeccccccc_bccc^__accccbcaaac^_bbaa]^abccccccaacbb`acccacccW
+@FCC2HFRACXX:7:1101:18318:59298#TGACCAAT/1
+CTGCATAGTACCATAGCTTTGTTTCTTACCATTGATTCAGTCCCGTTATTACTGATGGAGAGACATGAGACAAAATAAGCTACTGTTTGT
++
+___cccccSbc[`[[dg_^e^[befgh_gghbddddg[[a^dfd]ce`cacaXIXSXRaea\GaMWVM\bfhdc```YRH\Z_cZ^_b_`
+@FCC2HFRACXX:7:1101:10947:60766#TGACCAAT/1
+GGGTCGTTGAGGTTGATGCCGACCATGGCCTCCCCGAGTCCGGCGCTGACGTCGGCGAGGATGGTAGGGGCGCTGTAGTGGGGG
++
+abbeeeeegggggfhhiihihghhiiiihihhhiiihifbfhihgcccccaacccccTVVOGY_S]`acEOXXLTT]``bacEX
+@FCC2HFRACXX:7:1101:6033:61578#TGACCAAT/1
+GGAGCATCTGCGCGAGGCCGACCTTGACGGAGAAAGTCGGCGACTTGGGCTGCTTTGAGGGTTCCAGAAGGGCTGCGCCGTTGTTCCC
++
+_b^ce^ceggfgghghhhhhdhhhhhhhhhfhWbghZcgggbNZWWbba_^aaXbbbbaba[^WYGYSX]`a^W[WX_[XOT[W`S_`
+@FCC2HFRACXX:7:1101:9064:63291#TGACCAAT/1
+CATCATCCATGTTCCGGAGCGCACGGACATCGCCCATGACGGACCGCACATGCCTGACGGCCTCGACGATGTTGCCGGTCCCGGCCTCCCCC
++
+_[_ceeceggcgcghhfUedghffhhgghhhcgghhef`gfffedebbaa]_bbbbbbaaZaR`V]aaaa_`]_bSXaOT[a_VXTOW`aab
+@FCC2HFRACXX:7:1101:13801:64275#TGACCAAT/1
+GTGATGCAGACCAAGCAGCTGGGCCGCCTCCCTGTTGTGCAGTTCGCGGCCGGGGTGTGGCCACGCCTGCTGATGCCGCGCTCATGATGCAGCTT
++
+bbbeeeeefggggiiiiiiiiihehhhiffiidbcghhhhhhhhhhhhfecccccQVV^aaabc_]a_ac`__bbbbaaaa]a_bc]bcc``_R_
+@FCC2HFRACXX:7:1101:15417:65582#TGACCAAT/1
+ACTGAATCAATGGTAAGAAACAAAGCTATGGTACTATGCAGAACCTCTTTATTTTTGTGTCTTCTTATTAGCTACTGTATTAAGTTAGACTAGGCTCCCC
++
+bb_e^ccegeggghiifhiififhihhhhiibcgffhcgfhghhhiiihiiiihfhfefdgfhhhhhdhhihhhhfffd_dd]dddbddeecd[bbbacc
+@FCC2HFRACXX:7:1101:8403:66552#TGACCAAT/1
+CGGGGTGACGACGTCCATGATGACCCCGCCACGGAGCATCTGCGCGAGGCCGACCTTGACGGAGAAAGTCGCCGACTTGGGCTGCTTTGAGGGTTCCAGA
++
+abbeecceggggghfhhiiiiiiiiiihhighihfhihhggggeea`acacc[_aabbbbcaacV``b`baccaXX_acc^W^_abbccbcbaW[``ccR
+@FCC2HFRACXX:7:1101:10617:71584#TGACCAAT/1
+CGCGCTTTGGCCATGACGGGGATGGTGACGGCGCGCTTGATGTCGCGGATGAGGCCCGGGTCGGA
++
+bbbeeeeegfgggiiiiiiiiefhiYcScghfgfhgeeedddddcccaZ_ab_`[_c[_[HOV_O
+@FCC2HFRACXX:7:1101:12027:71848#TGACCAAT/1
+GCTTATTTTGTCTCATGTCTCTCCATCAGTAATAACGGGACTGAATCAATGGTAAGAAACAAAGCTATGGTACTATGCAGAACCTCTTTATTTTTGTGTC
++
+_bbeeeeeggeggihihhhhfhhiiiiihdeghdfeiihfhgihhhhhiihiaeghfhhhihiiifhdhhcdgggdgcbb]ceeebdddddcddcY`bcc
+@FCC2HFRACXX:7:1101:3054:72468#TGACCAAT/1
+CCAGCTGCTTGGTCTGCATCACCAAATCATACGGCGCGGCGATGCGCTTAGCATACGCGAACACCTCATCATCATCCATGTTCCGGAGCGCACGGACATC
++
+bbbeeeeeggggehhiiiiihiiiiihhiiiiiiihihidgabcaccccccccdccccccccaccccbccbccccccccbbbccaa_accc]accaaccc
+@FCC2HFRACXX:7:1101:14667:72258#TGACCAAT/1
+CGGGAATCTTCAAGAGTGGCGACCCTGCTCGCCGCGCGCGTGCCATCGTTCAGGCTGTCACCCACTACAGCGACCCTACCATCCT
++
+abbeeeeeggggghiccghhfhhhibeceXeghghgh[]`W^^aX`c^^aabccccaW_]`bc^a^X^]YYTZacTX[`^Y]ba_
+@FCC2HFRACXX:7:1101:1773:75211#TGACCAAT/1
+CTAGTCTAACTTAATACAGTAGCTAATAAGAAGACACAAAAATAAAGAGGTTCTGCATAGTACCATAGCTTTGTTTCTTACCATTGATTCAGTCCCGTTA
++
+bbbeeeeegggggihiiiihhiiiiiiiiiiiiiifhiiiiiiiiiiiiiffhiiiiiiiiiifhiiihiihhgfcghiggfggfeceeeeeddbdbccc
+@FCC2HFRACXX:7:1101:9079:79829#TGACCAAT/1
+GCTACAAATATATGAGAGAAGATGACGATGGTGGAGCGGATAGGGTTGTTCACTCGGATCTGGCGGCGTATCGCTCGACCTTAGGGTCGTTGAGGTTGAT
++
+bbbeeeeegggggiiiiiiiiiiihhihiiiafhfffhhffhafgaeg`f_dg]cgeadccV_`ba^ZET[^GQ[[TT__L]SGS[JXTTTXQSXGQRX`
+@FCC2HFRACXX:7:1101:5691:80221#TGACCAAT/1
+GTGCGGATCATGGCGGCGCCCTCACGGACCCTCCGGAGCGCCTCACCAAGGTCACGGCAGCCAC
++
+bbbeeeeegggggiiiffhgifgghhiageecac`__aacacccbacbcacX`bbacaZT[aW`
+@FCC2HFRACXX:7:1101:12818:82404#TGACCAAT/1
+CGGCGATAATTTTTGCATTACAAACAGTAGCTTATTTTGTCTCATGTCTCTCCATCAGTAATAACGGGACTGAATCAATGGTAAGAAACAAAGCTATGGT
++
+bbbeeeeegggggiiiiiiiihifhiihiiiiiiiiiiiiiiiiihiiihiihfhhhhhhiihhiihifgede_a_bdbbdbcbcbbbcc_acbccbcc`
+@FCC2HFRACXX:7:1101:11453:83437#TGACCAAT/1
+CGGGGATGGTGACGGCGCGCTTGATGTCGCGGATGAGGCCCGGGTCGGACATCCGCGCCACGCC
++
+abbeeeeegecgfhihfhiiiihhifihiefhacabddccc]acQWY_TaTX_bN^[_aTZTT^
+@FCC2HFRACXX:7:1101:2741:85193#TGACCAAT/1
+CCCGCATCGCGGAGGAAGCCGGCGCCTGCGCCGTCATGGTGCTGGAGCGCGTCCCCGCCGACATCCGCTCGCAGGGCGGCGTGG
++
+bbbeeeeefgggcghhhhhiiifffiigggeec_accbcbcbcccccbcccaccccaccaac[abcccacaaa_c[a[aa]T[_
+@FCC2HFRACXX:7:1101:11726:85018#TGACCAAT/1
+AAAAATTATTAAGAAGAGAAGGATGAAGCCGGCGGAGTGGAGATGCATATCGAAGGCAGATGGATGGATGGGTG
++
+_bbeeeeegggggihihhhiiffhiiiiiiiihii_gZbdbggfeeeeeebccc__a\_^R_b]_b_`Y`^cGO
+@FCC2HFRACXX:7:1101:19534:85665#TGACCAAT/1
+TGGATGGATGGGTGATACAAATATATGAGAGAAGATGACGATGGTGGAGCGGATAGGGTTGTTCACTCGGATCT
++
+__beccc^eege`ef]dcghhhhfcg]ggfgeghhhhccgcgdd_e[^c`Y`edagfdH^WX^VZUUUZ\N^_b
+@FCC2HFRACXX:7:1101:5108:90121#TGACCAAT/1
+CATGTCTCTCCATCAGTAATAACGGGACTGAATCAATGGTAAGAAACAAAGCTATGGTACTATGCAGAACCTCTTTATTTTTGTGTCTTCTTATTAGCTA
++
+bbbeeeeegggfgiiifgihhiiiihhhiiiiihiiiihfhhihhiiihiiiiiiiifgiiiihhiiihffddgggeeeeeeab_bddcccbccdcccbc
+@FCC2HFRACXX:7:1101:18115:92647#TGACCAAT/1
+CGACACAAAAATAAAGAGGTTCTGCATAGTACCATAGCTTTGTTTCTTACCATTGATTCAGTCCCGTTATTACTGATGGAGAGACATGAGACAAAATAAG
++
+bb_eeeeeggggghiihefegfhiiihiehhiffgghhgfheghihhiiifceggbhfhfhhidhehfaghfhggebgbdebecddcb_b_bbccccb``
+@FCC2HFRACXX:7:1101:2344:93442#TGACCAAT/1
+CAATGGTAAGAAACAAAGCTATGGTACTATGCAGAACCTCTTTATTTTTGTGTCTTCTTATTAGCTACTGTATTAAGTTAGACTAGGCTCCCCTGGTTTA
++
+bbbeeeeegggggiiiiiiihiiigihihiiiiiiiihiiiiifhihiiihiiiiihihhiihihihiiighiiddfhhcggggggceeecccdcc`bc^
+@FCC2HFRACXX:7:1101:6031:96678#TGACCAAT/1
+TACATGATAAACCAGGGGAGCCTAGTCTAACTTAATACAGTAGCTAATAAGAAGACACAAAAATAAAGAGGTTCTGCATAGTACCATAGCTTTGTTTCTT
++
+___eecc`g^eegfhhhidgdfhhcebhghdffdfhhiihcaegfhdghdfghhdhhihfic`dh]cggeg\Z]a^abbbcdcdbbbcb`_]Z_bccccb
+@FCC2HFRACXX:7:1101:15323:98549#TGACCAAT/1
+CAGGCGTGGCCACACCCCCGGCCGCGAACTGCACAACAGGGAGGCGGCCCAGCTGCTTGGTCTGCATCACCAAATCATACGGCGCGGCGATGCGCTTAGC
++
+bbbeeeeeggggghiiiiifgfhhhhhfeeeeedddccccccccbcccccccccccccbc_b`bbbcbccbc^ab]b`_b[_[]aXa]_]__^_aV_R`b
+@FCC2HFRACXX:7:1101:5063:100089#TGACCAAT/1
+TACAAATATATGAGAGAAGATGACGATGGTGGAGCGGATAGGGTTGTTCACTCGGATCTGGCGGCCTATCGCTCGACCTTAGGGTCGTTGAGG
++
+bbbeeeeegggggihiihihiiiiihheffghhiiiifegfhiaffghfhffdghffeeeeeccaWW^bGWW`a___aX_Y_`aJX_LXXP_`
+@FCC2HFRACXX:7:1101:16571:100277#TGACCAAT/1
+GCTTGATGTCGCGGATGAGGCCCGGGTCGGACATCCGCGCCACGCCGCCCTGCGAGCGGATGTCGGCG
++
+bbbeeeeeggggfhfhegbbfgifggV^_ffcUbdgaG\``^aaaaacccWaccacccccZZR_aa_a
+@FCC2HFRACXX:7:1102:8089:2409#TGACCAAT/1
+CAAAGCAGCCCAAGTCGGCGACTTTCTCCGTCAAGGTCGGCCTCGCGCAGATGCTCCGTGGCGGGG
++
+bbbeeeeeggfggihiiiiiiiiiifghhhfhhiiifghihigggeeccca`bcccccacccccac
+@FCC2HFRACXX:7:1102:10502:4703#TGACCAAT/1
+ATCCATTCCATTCCATTCCAACTCAATCCCCCCCCACCCAGCCCAAACAATGGCGTCGGACGGGAGCGGCGTGGTGACGGTGTACGGGAACAACGGCGCA
++
+bbbeeeeegggggiiiiiiihiihiiihhiihhiiiiiihfghidggggggedccccacccaccV]aac]aTX_GQY]_aT^O_bacc]a^`bQT]]ac]
+@FCC2HFRACXX:7:1102:12414:5238#TGACCAAT/1
+CGTACCATAGCTTTGTTTCTTACCATTGATTCAGTCCCGTTATTACTGATGGAGAGACATGAGACAAAATAAGCTACTGTTTGTAATGCAAAAATTATTA
++
+bbaeeeeegfgggiihiihiiiiiiihhhiihfihhihigfhiihhiiihiehigghiiihbgdhidgfhihhggdgdggeeeeededcdddcccbcddd
+@FCC2HFRACXX:7:1102:20475:6595#TGACCAAT/1
+CGTCCATGATGACCCCGCCACGGAGCATCTGCGCGAGGCCGACCTTGACGGAGAAAGTCGCCGACTTGGGCTGCTTTGAGGGTTCCAGAAGGGCTGCGCC
++
+bbaeeeeegggggihiiiiiiiiiihiiiiiiiiihihfggecccbbbcccccaccc]_^_ccacaacbccbacb_bb_acc_a^`bbcb]^aac]_caa
+@FCC2HFRACXX:7:1102:1964:7116#TGACCAAT/1
+GTCATCATGGACGTCGTCACCCCGGAACAAGCCCGCATCGCGGAGGAAGCCGGCGCCTGCGCCGTCATGGCGCTGGAGCGCGTCCCCGCCGACCTCCGCT
++
+bbbeeeeefeegfgghgibghiifgcgffhfggifhfhhiige[^acccccaaccccaacV[aZ_cOW]bccaaccccbXaaaaaacLX_[XaEO]`]aT
+@FCC2HFRACXX:7:1102:10385:11914#TGACCAAT/1
+TACAGCGACCCTACCATCCTCGCCGACGTCAGCGCCGGACTCGGGGAGGCCATGGTCGGCATCAACCTCAACGACCCTAAGGTCGAG
++
+bbbeeeeegggfgiiihiiiihhhhdhh`gfhdghdecccccccccTaaV^`bcc`b[aaaccb]babccbca[[T][^bbbYbXET
+@FCC2HFRACXX:7:1102:2463:14194#TGACCAAT/1
+GACTCGGGGAGGCCATGGTCGGCATCAACCTCAACGACCCTAAGGTCGAGCGATACGCCGCCAGATCCGAGTGAACAACCCTATCCGCTCCACCATCG
++
+__beeeeegcgfehhhfhdghiiihiiiiihhihihhihhggggeZadcccc^accacccccc_^bccccc^^]`_```ac_bbbccccccbbaabb^
+@FCC2HFRACXX:7:1102:6606:14993#TGACCAAT/1
+CCGTCAGGCATGTGCGGTCCGTCATGGGCGATGTCCGTGCGCTCCGGAACATGGATGATGATGAGGTGTTCGCGTATGCTAAGCGCATCGCCGCGCCG
++
+bbbeeeeegggfghiiihiiiiiiihiiegfgiiihihihhiiifggcecdddcbccbdcccccccY^Y^baccX]acccbbbRT]aaacccaccccc
+@FCC2HFRACXX:7:1102:13232:17115#TGACCAAT/1
+GGTACTATGCAGAACCTCTTTATTTTTGTGTCTTCTTATTAGCTACTGTATTAAGTTAGACTAGGCTCCCCTGGTTTATCATGTACTCAATCAAATGTTG
++
+_b^ceeeeggggghhihiiihghihiihfcghhdgiiihhgffhhfhheghhdgfaehi]ecbZbg`bbgf]_\\_b]ddgea_cddbabbbdbd]]TZ]
+@FCC2HFRACXX:7:1102:15049:17175#TGACCAAT/1
+CCCAAACAATGGCGTCGGACGGGAGCGGCGTGGTGACGGGGTACGGGAACAACGG
++
+___eeeecgggfehgfhihihihffghhedcbbU\`baaFRW_]XWWV[^_bGHO
+@FCC2HFRACXX:7:1102:12312:17759#TGACCAAT/1
+CGCCATGATCCGCACCAAGGGGGAGGCCGGGACCGGCAACATCGTCGAGGCCGTCAGGCATGTGCGGTCCGTCATGGGCGATGTCCGTGCGCTC
++
+_baeeeeegggggiiihhhiiiiiiihihhighigeeccccccccccccccccacaaccOY[Y`b_aOW]LJ[[[__a[_[]_cba^P^_ca]_
+@FCC2HFRACXX:7:1102:10932:21174#TGACCAAT/1
+GGGGAGGCCATGGTCGGCATCAACCTCAACGACCCTAAGGTCGAGCGATACGCCGCCAGATCCGAGTGAACAACCCTATCCGCTCCACCATCGTCATCTT
++
+bbbeeeeeggfggehiiiiiiiiiiiiihiihiiiiiiiighiiigeeebcccccccccacccccc^acdbcccccacccccccccccccbccW`W```b
+@FCC2HFRACXX:7:1102:14942:21184#TGACCAAT/1
+TCTTATTAGCTACTGTATTAAGTTAGACTAGGCTCCCCTGGTTTATCATGTACTCAATCAAATGTTGAACG
++
+^__ccccaec[`edfbcgfcdfeeb_gcaebf]_dfeceg_I^cf[eg_f^aXaceRcg_f]bc\bc\\__
+@FCC2HFRACXX:7:1102:10572:23384#TGACCAAT/1
+CTTGGTGAGGCGCTCCGGAGGGTCCGTGAGGGCGCCGCCATGATCCGCACCAAGGGGGAGGCCGGGACCGGCAACATCGTCGAGGCCGTCA
++
+_bbeecacgffceghhfhffhgWcfeffaegf`__acccccbbbccac^YaccccccaV__^acccc]a]Xaaaa[bb[Q[ETOXaaaX[^
+@FCC2HFRACXX:7:1102:4715:26873#TGACCAAT/1
+GCGCTTAGCATACGCGAACACCTCATCATCATCCATGTTCCGGAGCGCACGGACATCGCCCATGACGGACCGCACATGCCTGACGGCCTCGACGATGTTG
++
+bbbeeeeeggggfihiiiiiiiihhiiihiiiiiiiiiiichiiihggeeccccccbacccccbb_acccccccc_bcbccc`bcccacccccc[^a`b`
+@FCC2HFRACXX:7:1102:13003:30444#TGACCAAT/1
+GTCGGACATCCGCTCGCAGGGCGGCGTGGCGCGGATGTCCGACCCGGGCCTCATCCGCGACATCAAGCGCGCCGTCACCATCCCC
++
+___\accceggccfg`gheffbff_TX]b`UU\\_OXJWW[a_ab_aTWTW^__]]U_TTZTVY`bbb^]TTXaTO[_bbbbaaa
+@FCC2HFRACXX:7:1102:5899:31890#TGACCAAT/1
+CGCTCGCAGGGCGGCGTGGCGCGGATGTCCGACCCGGGCCTCATCCGCGACATCAAGCGCGCCGTCACCATCCCCGTCATGGCCAAAGCGCGCATCGGCC
++
+bbbeeecegggfghfhfhieefeaaacbb_^\aacaccaccccbcccaaccTTbcc`b^aaaccZ_acbGGX^[^XX^aWGRWb]a^[`_]a_a_aTXT[
+@FCC2HFRACXX:7:1102:4199:33606#TGACCAAT/1
+GGTGCATATCGAAGGCAGATGGATGGATGGGTGATACAAATATATGAGAGAAGATGACGATGGTGGAGCGGATAGGGTTGTTCACTCGGATCTGG
++
+ab^ceeeegggggiihgiefgheghi]feghYbbffhihf[chhfhhighhhifhihif\egi_fgU`dab]_abZ^K[\W^Y`b__^aTW[b_b
+@FCC2HFRACXX:7:1102:17457:38411#TGACCAAT/1
+CCATAAGCAGGGCTGGCAGAGCGACAGACATACAAACCGTTCAACATTTGATTGAGTACATGATAAACCAGGGGAGCCTAGTCTAACTTAATACAGTAGC
++
+bbbeeeeegfgggiiifhh\effacefff_d_cdfgfhhZafgfifdfggffgdbcV\`ddggeeeeeaba\`aWZ^ab]bY_bbbccccbccbcc__bc
+@FCC2HFRACXX:7:1102:10935:40565#TGACCAAT/1
+CGCTGTAGTGGGTGACAGCCTGAACGATGGCACGCGCGCGGCGAGCAGGGTCGCCACTCTTGAAGATTCCCGAGCCGACGAAGACGCCGTCGCACCCCAG
++
+__bee`c`bege^faghhhhihhihhhffhXcefhhfgffga_UZ[_baa[^aT^^abbbb`_bbY]`__bZ]Z[[[X_LT[a]]EOTXOXXOXaa^E[[
+@FCC2HFRACXX:7:1102:3735:42907#TGACCAAT/1
+TACCATCCTCGCCGACGTCAGCGCCGGACTCGGGGAGGCCATGGTCGGCATCAACCTCAACGACCCTAAGGTCGAGCGATACGCCGCCAGATCCGAGTGA
++
+_bbceeeeggggfihhhghiihdffhichhggggeaaa^acccc`b^acc_bbccccb``baaccc[bbccbccc_ac]`cc[__[_a[_`Y]baaaQ^b
+@FCC2HFRACXX:7:1102:3825:46331#TGACCAAT/1
+CCGGAGCGCACGGACATCGCCCATGACGGACCGCACATGCCTGACGGCCTCGACGATGTTGCCGGTCCCGGCCTCCCCCTT
++
+bbbeeeeegggggiiihhiiiigf]fgfhdfhiaddgeceead`baca_cccccacacccccc_aEW]a]]]a]cW_cc[`
+@FCC2HFRACXX:7:1102:1738:46716#TGACCAAT/1
+GGCTGGCAGAGCGACAGACATACAAACCGTTCAACATTTGATTGAGTACATGATAAACCAGGGGAGCCTAGTCTAACTTAATACAGTAGCTAATAAGAAG
++
+__beeeeegfgeghihhihiihhiihdghegbdffhiiiihhiihdebgfhihiiiiiiiigfdcab`bbb`bcc`bcdcccdccccdccccccbbbY]b
+@FCC2HFRACXX:7:1102:3022:48676#TGACCAAT/1
+CGAGCCGACGAAGACGCCGTCGCACCCAAGCTGCATCATGAGCGCGGCATCAGCAGGCGTGGCCACACCCCCGGC
++
+___cccccgeegghaffff^^YcFZeefg\dR_Z^dedd]^]RXR]WFTT^bXGY^^OTETVV^GW^aaaaaaaa
+@FCC2HFRACXX:7:1102:20821:54221#TGACCAAT/1
+GGCCCAGCTGCTTGGTCTGCATCACCAAATCATACGGCGCGGCGATGCGCTTAGCATACGCGAACACCTCATCATCATCCATGTTCC
++
+_bbccccegfeegehgffgf]g`egcfgbgeeffh`baggafdaUZaGRW_aa]bcccbcaaacaV^_^a_]_bc``_YGSY__`Y`
+@FCC2HFRACXX:7:1102:16360:55497#TGACCAAT/1
+GCAAAAATTATTAAGAAGAGAAGGATGAAGCCGGCGGAGTGGAGATGCATATCGAAGGCAGATGGATGGATGGGTGATACAAATATATGAGAGAAGATGA
++
+bbbeeeeegggggihhhhhihhiigffhibbcgiiii`gT^dgdccbc_bbdbbaccacca[]bb`]Y^^YbccXTWYa`bbbb`b`_]`b`b^bbb]Y`
+@FCC2HFRACXX:7:1102:5441:56789#TGACCAAT/1
+GTCAGCGCCGGACTCGGGGAGGCCATGGTCGGCATCAACCTCAACGACCCTAAGGTCGAGCGATACGCCGCCAGATCCGAGTGAACAACCCTATCCGCTC
++
+babeeeeegfgfgiiiiihhfhihhiiiegiiggeeeeedddddccccccccccc]`[_ccaaacaccaccZT]cbbbacc[abcccccccabbcccc_a
+@FCC2HFRACXX:7:1102:1305:59122#TGACCAAT/1
+CTGCATAGTACCATAGCTTTGTTTCTTACCATTGATTCAGTCCCGTTATTACTGATGGAGAGACATGAGACAAAATAAGCTACTGTTTGTAATGCAAAAA
++
+bbbeeeeeggggfiiiiihihhiiiiihhihiiiiiiiiihihhifhhiiiihihiiihifhfhiiiiihiiiiigdggeeeecedcdddddddcccbcc
+@FCC2HFRACXX:7:1102:1972:61139#TGACCAAT/1
+CCTCGACGAAGTGGCCGATGCGCGCTTTGGCCATGACGGGGATGGTGACGGCGCGCTTGATGTCGCGGGTGAGGCCC
++
+___eeceefgcggihifed[cbead_ghefdgH\cddgfgg^`bcZ]Z_\_^aacaEVWW`bb]Q^acETXQW^WQ^
+@FCC2HFRACXX:7:1102:8085:62091#TGACCAAT/1
+TTCAAGAGTGGCGACCCTGCTCGCCGCGCGCGTGCCATCGTTCAGGCTGTCACCCACTACAGCGACCCTACCATCCTCGCCGACGTCAGCGCCGGACTCG
++
+_bbeeeeegfgfgifiiifhhifhhdghaegd^bcabdccaaccbccaa`bcc`acca]bbcccaccccbc^]Yb[bccccaaccXRGT`aca_]aa[b_
+@FCC2HFRACXX:7:1102:6045:62449#TGACCAAT/1
+ATTCATAAGCCATAAGCAGGGCTGGCAGAGCGACAGACATACAAACCGTTCAACATTTGATTGAGTACATGATAAACCAGGGGAGCCTAGTCTAACTTAA
++
+bbbeeeeegfgggiihiiiiihihihfhhfdhfiihihiiiiiiiiiihhhiii_ddgcddgeecZ_ddddc`dbdbbccaaaaaaccbcccdccbbccc
+@FCC2HFRACXX:7:1102:14153:62735#TGACCAAT/1
+CACCCGTTATTACTGATGGAGAGACATGAGACAAAATAAGCTACTGTTTGTAATGCAAAAATTATTAAGAAGAGAAGGATGAAGCCGGCGGAGTGGAGAT
++
+___acc_cc^egeheafhdfgbfffdghhhfhhdaghhhfhdgbgh]cdfcfffh\dgfhhdfhhfghhfff`g^\bcebRZbb[__aaac[_WWWG[]^
+@FCC2HFRACXX:7:1102:6103:62753#TGACCAAT/1
+CCGGAACATGGATGATGATGAGGTGTTCGCGTATGCTAAGCGCATCGCCGCGCCGTATGATTTGGTGATGCAGACCAAGCAGCTGGGCCGCCTCCCTGTT
++
+bbbeeeeegggeghhiifiifiiefghihiihiihiiiiihiiiiiiiigecccc\^abbcedcb^abcccb`YW[`accccabbccac_accacc[b_b
+@FCC2HFRACXX:7:1102:18321:62766#TGACCAAT/1
+CCCGTTATTACTGATGGAGAGACATGAGACAAAATAAGCTACTGTTTGTAATGCAAAAATTATTAAGAAGAGAAGGATGAAGCCGGCGGAGTGGAGATGC
++
+bbbeeeeegggggiiihhihdfhiiiiihiiiiihiiiiiiiiifhiifgiiiiiifhdgfiihihihiiehihfdggceeeeeccccc[]Q[[Y`b`b_
+@FCC2HFRACXX:7:1102:6465:65486#TGACCAAT/1
+TACTGCATCACCAAATCATACGGCGCGGCGATGCGCTTAGCATACGCGAACACCTCATCATCATCCATGTTCCGGAGCGCACGGACATCGCCCATGACGG
++
+bbbeeeeefggegiiihiihiiihiiiiUedggfeecccbccdccccaccccccccbbccb`bcbbccccdccccc]accacccccc`bccccccbbcaa
+@FCC2HFRACXX:7:1102:15835:67354#TGACCAAT/1
+CTGCCTTCGATATGCATCTCCACTCCGCCGGCTTCATCCTTCTCTTCTTAATAATTTTTGCATTACAAACAGTAGCTTATTTTGTCTCATGTCTCTCCAT
++
+_b_c^cdccggcghihhfhhegdgdeghffa]aeegdcedfbfgf]`gd``ffdcgfhfdbdggabdea\abdc_Z_bb`cccbabcc_ZY]``]]`bcc
+@FCC2HFRACXX:7:1102:6768:67967#TGACCAAT/1
+ACATGATAAACCAGGGGAGCCTAGTCTAACTTAATACAGTAGCTAATAAGAAGACACAAAAATAAAGAGGTTCTGCATAGTACCATAGCTTTGTTTCTTA
++
+bbbeeeeefgggghiihiiiihiiiiihhghhhiiiiiieghiiiiiiiihhiiiiihhiiifdfhiggg`cceebcdddbbdcdccccccccccbcccc
+@FCC2HFRACXX:7:1102:15827:67868#TGACCAAT/1
+GCCTGACGGCCTCGACGATGTTGCCGGTCCCGGCCTCCCCCTTGGTGCGGATCATGGCGGCG
++
+bbbeeeeegggggiiihihhgdghiihfffh`ghhfhiiggac_a^adccU\accb`[acZa
+@FCC2HFRACXX:7:1102:1446:69132#TGACCAAT/1
+CAGACATACAAACCGTTCAACATTTGATTGAGTACATGATAAACCAGGGGAGCCTAGTCTAACTTAATACAGTAGCTAATAAGAAGACACAAAAATAAAG
++
+bbbeeeeegggggiihiihiiiiiihfhihiiffihiiiiiiiiffhiiiihiiiihfhiiiiiiegggggedeecdecddddddccccccccccabccb
+@FCC2HFRACXX:7:1102:1236:70725#TGACCAAT/1
+GGCGTCCATGATGACCCCGCCACGGAGCATCTGCGCGAGGCCGACCTTGACGGAGAAAGTCGCCGACTTGGGCTGCTTTGAGGGTTCCAGAAGGGC
++
+bbb`eeeeggfggiiiiiiiiiiiihiihhifhiiiihhg[ceccccccccccaaU`b]]_aacc_aa__bcc_a_bcb`_^baW[`b_bbbcc]^
+@FCC2HFRACXX:7:1102:9825:72205#TGACCAAT/1
+CCGACCCCGGAACAAGCCCTTCTGGAACCCTCAAAGCAGCCCAAGTCGGCGACTTTCTCCGTCAAGGTCGGCCTCGCGCAGATGCTCCGTGGCGGGG
++
+___cccecffegghihiiiihiiiifhfiihheghfhhfhiifgf_dfgacU^a__bccc\X^^Zbb]`acacXacXXaacT`bbcccc`^a][__a
+@FCC2HFRACXX:7:1102:6738:74232#TGACCAAT/1
+CTGGAGATGCATATCGAAGGCAGATGGATGGATGGGTGATACAAATATATGAGAGAAGATGACGATGGTGGAGCGGATAGGGTTGTTCACTCGGATCTGG
++
+bbbeeeeegggggiiiiiiieiefhhifgiigfhiicfefhiihihhiihiiihiihiiiihffcgfd^`b^cca]NZ_bccY^a]^Y`bc`[^OWT]Y_
+@FCC2HFRACXX:7:1102:3542:76333#TGACCAAT/1
+CCTCGACGAAGTGGCCGATGCACGCTTTGGCCATGACGGGGATGGTGACGGCGCGCTTGATGTCGCGGATGAGGCCCGGGTCGGACATCCGCGCCACGCC
++
+bbbeeeeefgfeghiiihhffbgffghiddghfiihiiiii_`gg__]adcca\__ac_`b_RY`aacTZa`bb^W^T[aET[XO[_]Y][aR[_ETX]a
+@FCC2HFRACXX:7:1102:8808:79183#TGACCAAT/1
+GGGCTTGTTCCGGGGTGACGACGTCCATGATGACCCCGCCACGGAGCATCTGCGCGAGGCCGACCTTGACGGAGAAAGTCGCCGACTTGGGCTGCTTTGA
++
+_bbeeeeeggggghhdeghiiiifgihf_ggffhihiiiggeeebcccccccbcccacccccaacacbccacca_^b`Y`[aaaccccbcca[X^b`bYS
+@FCC2HFRACXX:7:1102:19068:80610#TGACCAAT/1
+ATTCAGTCCCGTTATTACTGATGGAGAGACATGAGACAAAATAAGCTACTGTTTGTAATGCAAAAATTATTAAGAAGAGAAGGATGAAGCCGGC
++
+___eeeacccggcdghdhieghhfbgfdeefhdgiiiiihhfhehffgggdcbae]bcgcgbg]Ze`d`ffdbggb^`ceeee__bbb]b`Z_^
+@FCC2HFRACXX:7:1102:4722:82132#TGACCAAT/1
+TGCACCCAAGCTGCATCATGAGCGCGGCATCAGCAGGCGTGGCCACACCCCCGGCCGCGAACTGCACAACAGGGAGGCGGCCCAGCTGCTTGGTCTGCAT
++
+bbbeeeeegggggiiiiiihiiiihiiiiiiiiiiiiiiihiggggeedccc]_acccZZa[acccccca^W`cacaaccaca^[`_bc]bY^]_]bbb`
+@FCC2HFRACXX:7:1102:14968:85934#TGACCAAT/1
+ATCTGCGCCTCGACGAAGTGGCCGATGCGCGCTTTGGCCATGACGGGGATGGTGACGGCGCGCTT
++
+b__eeceeggggghhd_eefhidhe]dgfcgfafh`dggeddabecccFW^aY_Y`acT[V_VLO
+@FCC2HFRACXX:7:1102:17287:86478#TGACCAAT/1
+ACTACAGCGACCCTACCATCCTCGCCGACGTCAGCGCCGGACTCGGGGAGGCCATGGTCGGCATCAACCTCAACGACCCTAAGGTCGAGCGATACGCCGC
++
+bbbeeeeegggggiiiiiiiihifiiiiiiiiiiiiigeccccccccccccccabcc]bacZaccbccaccbbbc[]acb^^bbY_aaccca^acca[T[
+@FCC2HFRACXX:7:1102:9089:87630#TGACCAAT/1
+GGTCGCTGTAGTGGGTGACAGCCTGAACGATGGCACGCGCGCGGCGAGCAGGGTCGCCACTCTTGAAGATTCCCGAGCCGACGAAGACGCCGTCGCACCC
++
+bb_eeeeeggggchi`cfghffhiiihfhhfaffhhiiiiiigdccaccaba_JRVV__Zbbb`]`bb__R]aba]X_ac_ccaTQTXTLXTTX[]][_^
+@FCC2HFRACXX:7:1102:16408:97070#TGACCAAT/1
+CGAGGATGGTAGGGTCGCTGTAGTGGGTGACAGCCTGAACGATGGCACGCGCGCGGCGAGCAGGGTCGCCACTCTTGAAG
++
+_a^ceceefceggicfgfhhfghedbgH_^egfbddbcdfhihhfehhhhge^_accaT[LTXa]RRZVa_c`bb]Y]]b
+@FCC2HFRACXX:7:1102:12915:100079#TGACCAAT/1
+GCCCAAACAATGGCGTCGGACGGGAGCGGCGTGGTGACGGTGTACGGGAACAACGGCGCAG
++
+bbbceeeefgegaafehhhhiiihdfagfge]`b`abcca\aZb`cacaT[`bcaUaaLHO
+@FCC2HFRACXX:7:1102:8912:100688#TGACCAAT/1
+GCCGGGACCGGCAACATCGTCGAGGCCGTCAGGCATGTGCGGTCCGTCATGGGCGATGTCCGTGCGCTCCGGAACATGGATGATGATGAGGTGTTCGCGT
++
+bbbeeeeeggfgfiiiihiiiiffhhihfhhihfbdgbbcec`bcc`ac`bbaX_Xa_[]a[WOT]aZ]ZXZOOX^]bb]Y_bdbb`Y]`bX[_^baT]T
+@FCC2HFRACXX:7:1103:9482:4541#TGACCAAT/1
+TGGAGCGGCGTGGTGACGGTGTACGGGAACAACGGCGCAGCCCTTCTGGAACCCTCAAAGCAGCCCAAGTCGGCGACTTTCTCCGTCAAGGTCGGCCTCG
++
+bbbeeeeegggggegdhiicffgiiiihifhifddgccccccccccccccbcccccabcbbcb_acaca_baccca_cccbcbcccacab`_bLXaccaa
+@FCC2HFRACXX:7:1103:4835:5055#TGACCAAT/1
+ATTTGGTGATGCAGACCAAGCAGCTGGGCCGCCTCCCTGTTGTGCAGTTCGCGGCCGGGGGTGTGGCCACGCCTGCTGATGCCGCGCTCATGATGCAGCT
++
+a_beeeccgggggiiiihiiiiiiiiiihiiiihiiiiighhiiiihiiiihgeccccbccTZZ_ca^ccc_a`a^`b``bccccccac_b`_bcc``cb
+@FCC2HFRACXX:7:1103:16692:6719#TGACCAAT/1
+GGGCGCCGCCATGATCCGCACCAAGGGGGAGGCCGGGACCGGCAACATCGTCGAGGCCGTCAGGCATGTGCGGTCCGTCATGGGCGATG
++
+__beeeeeggggghiiiiiiiiehghiih`ghiggecacccccccccccccccWaacca_aacb^W]`YYYT]OXXaaaGW]baacLHT
+@FCC2HFRACXX:7:1103:6301:12756#TGACCAAT/1
+CCCACTACAGCGACCCTACCATCCTCGCCGACGTCAGCGCCGGACTCGGGGAGGCCATGGTCGGCATCAACCTCAACGACCCTAAGGTCGAGCGATACGC
++
+___ceeeeggfggihhdhhgdhichffhhifddeegdfdaaaaaccccaaaaca^_cbcb]__c]_a]Y`ba`bb_baccccc^^bb]`a[aX]_a_a[a
+@FCC2HFRACXX:7:1103:18555:25778#TGACCAAT/1
+CTTGGTGAGGCGCTCCGGAGGGTCCGTGAGGGCGCCGCCATGATCCGCACCAAGGGGGAGGCCGGGACCGGCAACAT
++
+___eeccefef^efhhhfgiiieggf_c`eghdfdgecb]a`b`bcacaaaWW^accccaccccccZ]a]_cc]WW^
+@FCC2HFRACXX:7:1103:11828:26565#TGACCAAT/1
+CGATGGTGGAGCGGATAGGGTTGTTCACTCGGATCTGGCGGCGTATCGCTCGACCTTAGGGTCGTTGAGGTTGATGCCGACCATGGCCTCCC
++
+_bbeeeccgceggiaghiiiegafgifihfhi`dgdfhdfhU`^__a^[^aZOV_T__]`aRROT]^Q]^X^^GSSY`LHO]TYRRW^Y[`_
+@FCC2HFRACXX:7:1103:11304:31167#TGATCAAT/1
+TGGGGCCACGCCTGCTGATGCCGCGCTCATGATGCAGCTTGGGTGCGACGGCGTCTTCGTCGGCTC
++
+bbbeeeeegfgggiiiiiiiiiiihiiiiihiiiiiiiiihih_dgadgdccc\accbcb^aca^_
+@FCC2HFRACXX:7:1103:16017:31664#TGACCAAT/1
+GTCTTCGTCGGCTCGGGAATCTTCAAGAGTGGCGACCCTGCTCGCCGCGCGCGTGCCATCGTTCAGGCTG
++
+bbbeeeeeggggghgiidfdcfg]cf[ecIXcaffagh]\`XZLYaFRRZRXWLTT`b]]QW^[XY`^PQ
+@FCC2HFRACXX:7:1103:1830:33244#TGACCAAT/1
+CACAACAGGGAGGCGGCCCAGCTGCTTGGTCTGCATCACCAAATCATACGGCGCGGCG
++
+^^_^acc^ee`c`edWccfaGaRaed]ae\eeeeedhe]`RMG\dec]ac\_aaaW[a
+@FCC2HFRACXX:7:1103:10914:36685#TGACCAAT/1
+TGGTGAGGCGCTCCGGAGGGTCCGTGAGGGCGCCGCCATGATCCGCACCAAGGGGGAGGCCGGGACCGGCAACATCGTCGAGGCCGTCAGGCATGTGCGG
++
+abbeeeeefggggiiiihiicfghegfhihhhhgfeccccccccccccccaccccccccccccccaccccc_cab`a`aaaaccccX_OTb[^`aY]YX[
+@FCC2HFRACXX:7:1103:8436:37721#TGACCAAT/1
+CGGCGTGGTGACGGTGTACGGGAACAACGGCGCAGCCCTTCTGGAACCCTCAAAGCAGCCCAAGTCGGAGACTTTCTCCGTC
++
+_^_ccW_c`ecgeg^dfgff`f[e`e]Z__U_e_``ZZdc]__bbbbbaaa^b_`[bbaQ[[[^`bWVET_^`_`bRY_TGQ
+@FCC2HFRACXX:7:1103:3640:39304#TGACCAAT/1
+TGACGGTGTACGGGAACAACGGCGCAGCCCTTCTGGAACCCTCAAAGCAGCCCAAGTCGGCGACTTTCTCCGTCAAGGTCGGCCTCGCGCA
++
+_bbeeeeegggggiiifgifhhdgfhhiiifhhihffhhifgggcccce`db`aaa`Z[^_aTTVabbY_RXW`[[`b]`aT[_[`X[[[a
+@FCC2HFRACXX:7:1103:20809:40434#TGACCAAT/1
+CTTGGTGAGGCGCTCCGGAGGGTCCGTGAGGGCGCCGCCATGATCCGCACCAAGGGGGAGGCCGGGACCGGCAACATCGTCGA
++
+___ec`ccggggghiiihg]gheceac_VbffegU\]]`^abbbbR^^ZWVWW[_^a_[acW[_a]LOQTXXLHQ[RY^aW[T
+@FCC2HFRACXX:7:1103:10701:49554#TGACCAAT/1
+CTCCGCGATGCGGGCTTGTTCCGGGGTGACGACGTCCATGATGACCCCGCCACGGAGCATCTGCGCGAGGCCGACCTTGACGGAGAAAGTCGCCGACTTG
++
+_bbeeeeccegggfheg[aecccghcZ``fddeeX]a^cccbccccc__aaaaaa[aa[_bbbbcacaacccc]_aabbbb]a]aX[bb_]^X_aMX__R
+@FCC2HFRACXX:7:1103:18885:50545#TGACCAAT/1
+CAATGGTAAGAAACAAAGCTATGGTACTATGCAGAACCTCTTTATTTTTGTGTCTTCTTATTAGCTACTGTATTAAGTTAGACTAGGCTCCCCTGGTTTA
++
+bbbeeeedggggghihhiiiihhidggbghiiiiiihhhihiiifhiihgdgghihhfhiiiiiagdbhieghiicgghifggggddeaccabbac`Z^]
+@FCC2HFRACXX:7:1103:5631:52141#TGACCAAT/1
+CCGCTCGCAGGGCGGCGTGGCGCGGATGTCCGACCCGGGCCTCATCCGCGACATCAAGCGCGCCGTCACCATCCCCGTCATGGCCAAAGCGC
++
+___ec_cegggcghhffghf[dd^`ZU\b]b^_aaaaY_TYW^`_b_WaXETX[b]bb][RVVaaWZ^a]bbbP[_XEWQ[bb^b`GX[XX_
+@FCC2HFRACXX:7:1103:2625:53946#TGACCAAT/1
+CCCTGTTGTGCAGTTCGCGGCCGGGGGTGTGGCCACGCCTGCTGATGCCGCGCTCATGATGCAGCTTGGGTGCGACGGCGTCTT
++
+a__eeeecg`egghfghhihhiihihgS\Z_aaac___ccaccccbb_baaacccW`bbccccb_]``aa^]WTT[[XTXXXX_
+@FCC2HFRACXX:7:1103:7468:59032#TGACCAAT/1
+GCTGCTGTTTGTAATGCAAAAATTATTAAGAAGAGAAGGATGAAGCCGGCGGAGTGGAGATGCATATCGAAGGCAGATGGATGGATGGGTGATACAAATA
++
+bbbeeeeeggggehiiihiiiihifgffhhhiihiiiiiiiiiihhhfiihgX`Z]ababb_bbcbbcccccbcc_ac__]bc[^b`acRX[]Y]`b]_Y
+@FCC2HFRACXX:7:1103:2609:67593#TGACCAAT/1
+CAACGGCGCAGCCCTTCTGGAACCCTCAAAGCAGCCCAAGTCGGCGACTTTCTCCGTCAAGGTCGGCCTCGCGCAGATGCTCCGTGGCGGGG
++
+abbeeeeegggggiihiiiihiiihiihiiihhfiiiiiifghhgecccccccccc`cccccbcccaccccccc]_Xbcbbcccccaccccc
+@FCC2HFRACXX:7:1103:8738:68263#TGACCAAT/1
+CGAGGATCTGCGCCTCGACGAAGTGGCCGATGCGCGCTTTGGCCATGACGGGGATGGTGACGGCGCGCTTGATGTCGCGGATGAGGCCC
++
+bbbeeeeegggggihiiiiiiiighghfhgafhhhhicggeeeecddddccccZ^acY_`bac[aaca]]^^bbY]`^]aOT[_bbO^^
+@FCC2HFRACXX:7:1103:9120:68970#TGACCAAT/1
+GCGCCGCCATGATCCGCACCAAGGGGGAGGCCGGGACCGGCAACATCGTCGAGGCCGTCAGGCATGTGCGGTCCGTCATGGGCGATGTCCGTGCGCTCCG
++
+bbbeeeeegggggiiiihihihhiiii`ghiiiggecccacccccccacccccccccXaccccabcccbcaXX_X^`ac`b_P]XX_`bb^GQXT_]_aa
+@FCC2HFRACXX:7:1103:20269:70791#TGACCAAT/1
+GTATTAAGTTAGACTAGGCTCCCCTGGTTTATCATGTACTCAATCAAATGTTGAACGGTTTGTATGTCTGTCGCTCTGCCAGCCCTGCTTATGGCTTATG
++
+_bbeceeegggggiiidghhfiiihih[ef`ecfhifhhegiiiihdfdf^cedce^daefbfghfddfbg_gdd^`^ceb_bcb_`abc[_]bKTQY_R
+@FCC2HFRACXX:7:1103:10802:71133#TGACCAAT/1
+GTGGCCACGCCTGCTGATGCCGCGCTCATGATGCAGCTTGGGTGCGACGGCGTCTTCGTCGGCT
++
+a__eccecgegfgiiihhhihhfhifghiehfffdcghhiif_eZ_fgccb^^accbab_Za[_
+@FCC2HFRACXX:7:1103:2290:74271#TGACCAAT/1
+GTGGGTGACAGCCTGAACGATGGCACGCGCGCGGCGAGCAGGGTCGCCACTCTTGAAGATTCCCGAGCCGACGAAGACGCCGTCGCACCCAAGCTGCATC
++
+_bbeeeeegeefgiiiffcgXafddgiihihifhgccccccccW`accccbcccccbccccbcbW]]cccccaccaa`X_aaXaacc]_caGGW]S]bbc
+@FCC2HFRACXX:7:1103:6528:76684#TGACCAAT/1
+TCGACGCCGACGGCCTCGAGGATCTGCGCCTCGACGAAGTGGCCGATGCGCGCTTTGGCCATGACGGGG
++
+bbbeeeeeggfeghiiighihihihihiiiigggedcWa\bbccc\ZYVOOT[LYaR]bbbb__baccc
+@FCC2HFRACXX:7:1103:7705:81342#TGACCAAT/1
+CAGGGCTGGCAGAGCGACAGACATACAAACCGTTCAACATTTGATTGAGTACATGATAAACCAGGGGAGCCTAGTCTAACTTAATACAGTAGCTAATAAG
++
+bbbeeeeegggggihiiihhihihhhiihiiighigihhhiihhhihiifhhiihhhhiiggfgeeeccccccc`]bccccdccccccccdccccbbbbb
+@FCC2HFRACXX:7:1103:17079:81373#TGACCAAT/1
+CCGCTCGCAGGGCGGCGTGGCGCGGATGTCCGACCCGGGCCTCATCCGCGACATCAAGCGCGCCGTCACCATCCCCGTCATGGCCAAAGCGCGCATCGGC
++
+bbbeeeeeggfgfiihihiiegeeccccbceaacccccacccccbcccccacaccc_bb_caccaaacccccccc^XO^^[b`accccc^]aacXX[_aa
+@FCC2HFRACXX:7:1103:15390:86731#TGACCAAT/1
+GCTGTAGTGGGTGACAGCCTGAACGATGGCACGCGCGCGGCGAGCAGGGTCGCCACTCTTGAAGATTCCCGAGCCGACGAAGACGCCGTCGCACCC
++
+bbbeeeeegggegfhiihiiiiiiiihiiiiiiehiiiigec\a_cbac[ba_[QYbb_`Y]b`_bcccccTX_acca]T[WTGOX[XXX]aRX[_
+@FCC2HFRACXX:7:1103:11631:90555#TGACCAAT/1
+CCGGCGCTGACGTCGGCGAGGATGGTAGGGTCGCTGTAGTGGGTGACAGCCTGAACGATGGCACGCGCGCGGCGAGCAGGGTCGCCACTCTTGAAGATTC
++
+bbbeeeeegggfgiihiifgiZ_eg\bbggHV_abcbbc_bac^a`bccaabcccccWa_Z`bbaccca]_a]aTX[T[^[RX[]WV]_bb]]SYb___R
+@FCC2HFRACXX:7:1103:9768:91822#TGACCAAT/1
+GTCGACGAGAGCGAGGTCCTCACACCCGCCGACGACGCGCACCACATCAACAAGCACAACTTCCGTGTCCCCTTCGTCTGTGGCTGCCG
++
+___eeeeegfffggfhfff`ghfhiiiffhf`ffee`bccccccccbcbbccab[`bc[_b`bcV_aaaY_abW^^b_aacb^a`aO^X
+@FCC2HFRACXX:7:1103:10253:96457#TGACCAAT/1
+AAGAAACAAAGCTATGGTACTATGCAGAACCTCTTTATTTTTGTGTCTTCTTATTAGCTACTGTATTAACTTAGACTAGGCTCCCC
++
+___cccccggee^dcffQ[b`ac]`c^[bbbgfbfdgeXceg]gfd^f_ed_efhc^_[e\ae\ebbfbHW\VVb\\bHVVZ`PTS
+@FCC2HFRACXX:7:1103:17931:96785#TGACCAAT/1
+GTGACGGCGCGCTTGATGTCGCGGATGAGGCCCGGGTCGGACATCCGCGCCACGCCGCCCTGCGAGCGGATGTCGGCGGGG
++
+_a_eeeecgfggghghhhiffffh`d[bcdddgU`bX]\a^\L]bbXaccaW[[[cTTZaLW^[OWWTVOTXS]POXX]aa
+@FCC2HFRACXX:7:1104:12013:3094#TGACCAAT/1
+CCACACCCCCGGCCGCGAACTGCACAACAGGGAGGCGGCCCAGCTGCTTGGTCTGCATCACCAAATCATACGGCGCGGCGATGCGCTTAGCATACGCGAA
++
+__[ccc^c`eceYbe^eU_eQ_\be`bdeeee^[c^^^aV_QQWW``a[]^X_a^]R]YY`^]^^_]RY`YW^XaX[_T]X]a_a_T[]aYGS]`aaaRT
+@FCC2HFRACXX:7:1104:7657:9443#TGACCAAT/1
+ATTCCATTCCATTCCAACTCAATCCCCCCCTACCCAGCCCAAACAATGGCGTCGGACGGGAGCGGCGTGGTGACGGTGTACGGGAACAACGGCGCAGCCC
++
+bbbeeeeegggggiiiiiihiiiiiiiiiiifhiiiiihiiiiiifhhhhiggecccccc_acccacacc^_]b^]T`[bccacT]^acc_caccR]X`a
+@FCC2HFRACXX:7:1104:18693:12541#TGACCATC/1
+GCCGTCGCACCCAAGCTGCATCATGAGCGCGGCATCAGCAGGCGTGGCCACACCCCCGGCCGCGAACTGCCCAACAGGGCGGCGGC
++
+[__ea_`cegfgfhhhhhhhhehhhhhhhhgfhhhhhhfffddcdN\acbcac^_caTTZ[V_acVV^`_GW``^^GWWGTT[_c_
+@FCC2HFRACXX:7:1104:1737:13058#TGACCAAT/1
+CCTCACCAAGGTCACGGCAGCCACAGACGAAGGGGACACGGAAGTTGTGCTTGTTGATGTGGTGCGCGTCGTCGGCGGG
++
+bbbeceeeggggfhffiagffhaedbffgegfggh[eg_ffffd\b^\P^_daad_U]`U]`T^_WQZWX_OWXXTT[_
+@FCC2HFRACXX:7:1104:9247:13508#TGACCAAT/1
+GGTCGCTGTAGTGGGTGACAGCCTGAACGATGGCACGCGCGCGGCGAGCAGGGTCGCCACTCTTGAAGATTCCCGAGCCGACGAAGA
++
+bbbeeeeegggaghi_eegfhiihiiiihhhhhihiiihhhifecaaaW_baa^baacccbcbccbbbccbc`b[]]aa__ca_EQO
+@FCC2HFRACXX:7:1104:19057:13842#TGACCAAT/1
+GGTCGCTGTAGTGGGTGACAGCCTGAACGATGGCACGCGCGCGGCGAGCAGGGTCGCCACTCTTGAAGATTCCCGAGCCGACGAAGACGCCGTCGCACCC
++
+bbbeeeeeggfegiiffhiiiiiifhiihhhhihiiifhhghWN]aYaacbc_XX_[a^acbbcbbY]__]`cca_aa]a__aaR^]b]aaXOX]]XR[^
+@FCC2HFRACXX:7:1104:6652:34019#TGACCAAT/1
+GTTCGCGTATGCTAAGCGCATCGCCGCGCCGTATGATTTGGTGATGCAGACCAAGCAGCTGGGCCGCCTCCCTGTTGTGCAGTTCGCGGCCGGGGGTGTG
++
+_abeeeeegggggiihhiifhiffffhhiiiagdgbdgeee^cdbbdcdcacccca`bcbcbacccccccccccYYbbacccbbc_accccaccccX_TW
+@FCC2HFRACXX:7:1104:9656:40471#TGACCAAT/1
+CGCCACTCTTGAAGATTCCCGAGCCGACGAAGACGCCGTCGCACCCAAGCTGCATCATTAGAGC
++
+__[ace`ccg^egffffhffgcdd_ffdefeefZeU`_`aW^cbbbaXKZ`bbbbb_bGZYGKT
+@FCC2HFRACXX:7:1104:4882:43361#TGACCAAT/1
+CCGCGACATCAAGCGCGCCGTCACCATCCCCGTCATGGCCAAAGCGCGCATCGGCCACTTCGTCGAGGCGCAGATCCTCGAGGCCGTCGGCGTCGACT
++
+bbbeeeeegggggfhegihiihiiiiiiiihiiiiigggggeeeebcccccccc_aa^bbca^^aa]_]acc__`^bbb_aa__a_Xa]XXTET[T[a
+@FCC2HFRACXX:7:1104:1688:50861#TGACCAAT/1
+GTCTGCATCACCAAATCATACGGCGCGGCGATGCGCTTAGCATACGCGAACACCTCATCATCATCCATGTTCCGGAGCGCACGGACATCGCCCATGACGG
++
+___ee`cegffefhihd`gfffggiihiaefgegeeccbbcbccccaccccccccccccccccccbbbbc_bcccR[aaacccccacccccccccb]bW]
+@FCC2HFRACXX:7:1104:1814:55634#TGACCAAT/1
+CTCGAGGATCTGCGCCTCGACGAAGTGGCCGATGCGCGCTTTGGCCATGACGGGGATGGTGAC
++
+_bbeeeeeegcceegfdgghfhiigfhibfgUV_e_fgedeabbb``]b_b^^\aUX_VRYGY
+@FCC2HFRACXX:7:1104:13964:57743#TGACCAAT/1
+GTGACCTTGGTGAGGCGCTCCGGAGGGTCCGTGAGGGCGCCGCCATGATCCGCACCAAGGGGGAGGCCGGGACCGGCAACATCGTCGAGGCCGCCAGG
++
+bbbeeeeegggegiiafgiihiiiiihaegifhiihfiggecaca__bbccccacccccccaaa]aaaccacX_RX]aaPPXSWWQ[XOTXWX[][X^
+@FCC2HFRACXX:7:1104:15970:65750#TGACCAAT/1
+CCACCATCGTCATCTTCTCTCATATATTTGTATCACCCATCCATCCATCTGCCTTCGCTATGCATCTCCACTCCGCCGGCT
++
+^__cPccc^YQQ`bcfhh][eXg``aad__[bgdcI^Y^ceeRa^aeacc[e[aeebGVbObehbfSbdS\V\^P\^[KFR
+@FCC2HFRACXX:7:1104:5753:69503#TGACCAAT/1
+TGCTAAGCGCATCGCCGCGCCGTATGGTTTGGTGATGCAGACCAAGCAGCTGGGCCGCCTCCCTGTTGTGCAGTTCGCGGCCGGGGGTGTGGCCACGCCT
++
+bbbeeeeegggggiiiifiiihghhiRefhhicfgiiiigggggeeeeddbdccccccccccccccccc^`bbbbbccccccaccacHTT^a`abacac`
+@FCC2HFRACXX:7:1104:8594:70272#TGACCAAT/1
+CGCTCGCAGGGCGGCGTGGCGCGGATGTCCGACCCGGGCCTCATCCGCGACATCAAGCGCGCCGTCACCATCCCCGTCATGGCCAAAGCGCGAATC
++
+___cee`ceccecf[``fheddcaRRZ_bcaaa_VXOROV_`b]`]T^a[aaaX]b_baaaZRTT_W^bbbbW[^XX[JQYY[XXQ[^RXXaEOT^
+@FCC2HFRACXX:7:1104:6170:79864#TGACCAAT/1
+GGCCGATGCGCGCTTTGGCCATGACGGGGATGGTGACGGCGCGCTTGATGTCGCGG
++
+__beee`caecgghfhfhefhh[^cccghOFZeM_eggdda`a\\^a^`b]X`aaW
+@FCC2HFRACXX:7:1104:12071:82778#TGACCAAT/1
+GTGAGGACCTCGCTCTCGTCGACGTAGTCGACGCCGACGGCCTCGAGGATCTGCGCCTCGACGAAGTGGCCGATGCGCGCTTTGGCCATGACGGG
++
+b_beeeeegggffhiiihhhhiihfgihiiiiiiihagcbccacccccaccbccca_[_a_caZ_a^bcaaaLTX^LOT[]]aGXY]GXYY_^_a
+@FCC2HFRACXX:7:1104:20929:87203#TGACCAAT/1
+ATCTGCCTTCGATATGCATCTCCACTCCGCCGGCTTCATCCTTCTCTTCTTAATAATTTTTGCATTACAAACAGTAGCTTATTTTGTCTCATGTCTCTCC
++
+bbbeeeeegggggihihhhhhiiihiihidffhfhhcghcghbghfffhhiffdZbcg`dgd^cbaZ^_]_`b_Z]Z]_bbb_``Z[]`bb_]ZGZ]``b
+@FCC2HFRACXX:7:1104:5982:97045#TGACCAAT/1
+GGAACAAGCCCGCATCGCGGAGGAAGCCGGCGCCTGCGCCGTCATGGCGCTGGAGCGCGTCCCCGCCGACATCCGCTCGCAGGGCGG
++
+bbbeeeeegfgggiifdhiiaghhhfffegUaecccdccacZ^_accaccT[_`[[_aaLT[_accca]aR]_][WX]X]aa[_aca
+@FCC2HFRACXX:7:1104:19682:99500#TGACCAAT/1
+GCAGAACCTCTTTATTTTTGTGTCTTCTTATTAGCTACTGTATTAAGTTAGACTAGGCTCCCCTGGTTTATCAT
++
+b__eeeeegeggghihdehfdca_ggdgibgiffihihihihcfhgfhhhgf[XcR^egfhaacdgbf]\_V\b
+@FCC2HFRACXX:7:1105:7757:3083#TGACCAAT/1
+CCGACATCCGCTCGCAGGGCGGCGTGGCGCGGATGTCCGACCCGGGCCTCATCCGCGACATCAAGCGCGCCGTCACCATCCCCGTCATG
++
+b__eeeeefggecceefhhaefhdUacb[^^_UETWW][PaaaRYWV_^a`_]`N^_T[[abbbbR[LWZ]TTZ^W]Y_baaa][aOGR
+@FCC2HFRACXX:7:1105:5894:5164#TGACCAAT/1
+CCCCAACCCTCCTCTTCTCCCTCCCCTGACTGGAGAGAGAGAACCTGAACCTTCCCGAGAGGATAGATAGGGGGGACGGAAGGCACCCGCATCGAGG
++
+b__ceeeeggggghhhhihhhgfhihbhfhiiifhfhieffgiffihihihfhihfhadad^adbdb]_]bZ]Y[T[_LO^T^W`GW[aaLXR]baa
+@FCC2HFRACXX:7:1105:7646:10607#TGACCAAT/1
+CCGGGCCTCATCCGCGACATCAAGCGCGCCGTCACCATCCCCGTCATGGCCAAAGCGCGCATCGGCCACTTCGTCGAGGCGCAGATCCTCGAGGCCGTCG
++
+bbbeeeeeggggghiiiiiiiiihiiihiiihggggeeeeeccccccccccccccbcccccccccccccbccccaa_accccccccccccaa[]M^_X_]
+@FCC2HFRACXX:7:1105:1938:27512#TGACCAAT/1
+GTGCGGATCATGGCGGCGCCCTCACGGACCCTCCGGAGCGCCTCACCAAGGTCACGGCAGCCACAGACGAAGGGGACAC
++
+babeeeeegggggihihihiifhhhhhgdee_ab`a^_caaccccccbWW^R_bc_aNT_G[G[`[^`LTZQX]cXX^^
+@FCC2HFRACXX:7:1105:3028:31211#TGACCAAT/1
+CCGTCACCATCCCCGTCATGGCCAAAGCGCGCATCGGCCACTTCGTCGAGGCGCAGATCCTCGAGGCCGTCGGCGTCGACTACGTC
++
+bbbeeeeegggggiiiihiiiiiiiihhhhiiihiihiiiiigggggeccc]accaacccccccaccccaacaXX_[XOXW[^WW`
+@FCC2HFRACXX:7:1105:8571:43765#TGACCAAT/1
+AGGAAGCCGGCGCCTGCGCCGTCATGGCGCTGGAGCGCGTCCCCGCCGACATCCGCTCGCAGGGCGGCGTGGCGCGGATGTCCGACCCGGGCCTCATCCG
++
+bb_eeeeegfgfgihhihihceghfhihhiffgggeeccccccaacccccbcccccccZa_[accc_a]]_acccaTT[[Y_ca]_acXXXX_aab]Y`T
+@FCC2HFRACXX:7:1105:12879:48945#TGACCAAT/1
+TGAGGACCTCGCTCTCGTCGACGTAGTCGACGCCGACGGCCTCGAGGATCTGCGCCTCGACGAAGTGGCCGATGCGCGCTTTGGCCATGACGGGG
++
+bbbeeeeegggggiiiiiiiiiifhfhiihiiiiiffecccccacccaccccbaccccccccacccccaacRX]^]a_]]acbbbcbbbRSQ[_a
+@FCC2HFRACXX:7:1105:20628:62425#TGACCAAT/1
+CATCATTCATAAGCCATAAGCAGGGCTGGCAGAGCGACAGACATACAAACCGTTCAACATTTGATTGAGTACATGATAAACCAGGGGAGCCTAGTCT
++
+_^_eaccaeacagcghdfggfdfffffgdgcf\ceggee`fcgddghhf`efbcbgdg`db]_VZZ^ZZMG]`ZUUTZ]`T\^QT\aaa]^]R^GSY
+@FCC2HFRACXX:7:1105:3594:73851#TGACCAAT/1
+CGAGAGCGAGGTCCTCACACCCGCCGACGACGCGCACCACATCAACAAGCACAACTTCCGTGTCCCCTTCGTCTGTGGCTGCCGTG
++
+_bbeeeeeeegggihiihifiiiiihecfhfhgdccc_aaabcc]^bcc^`X[`a_bc]a]W^ab```acc[__c_]ba^a^`aET
+@FCC2HFRACXX:7:1105:19869:79727#TGACCAAT/1
+GTCGGCGTCGACTACGTCGACGAGAGCGAGGTCCTCACACCCGCCGACGACGCGCACCACATCAACAAACACAACTTCC
++
+__beeeecegeeedegcdcfg_efaYefg_dFV^bedGVZ\`]aca^XVOTTY_cQaaW^^Y`Y]bbcGWWGW^^]]GS
+@FCC2HFRACXX:7:1105:6280:84664#TGACCAAT/1
+CGACGCGCACCACATCAACAAGCACAACTTCCGTGTCCCCTTCGTCTGTGGCTGCCGTGACCTTGGTGAGGCGCTCCGGAGGGTCCGTGAGGGCGCCGCC
++
+bb_eeeeeggfggiihhfiiiihfhiiiiiihhhiiiiiihiihhihgegggeeaa^\ZZ^bGZ_bY^]X[^]Z_aT]aTaacWX^][_GW`_]a[a[T[
+@FCC2HFRACXX:7:1105:10628:85592#TGACCAAT/1
+CGGCCACTTCGTCGAGGCGCAGATCCTCGAGGCCGTCGGCGTCGACTACGTCGACGAGAGCGAGGTCCTCACACCCGCCGACGACGCGCACCACATCA
++
+bbbeeeeegggfgiiiiiiiiiiihifhhighfgigggeccaccccaac^`aWaaU[UV[`[ZZaT[bbcbabbca]aca_aaL[Xaa_aac[`^]b`
+@FCC2HFRACXX:7:1106:10519:3964#TGACCAAT/1
+CCTCGATGCGGGTGCCTTCCGTCCCCCCTATCTATCCTCTCGGGAAGGTTCAGGTTCTCTCTCTCCAGTCAGGGGAGGGAGAAGAGGAGGGTTGGGGGGG
++
+bbbeeeeeggggeghihiiidghiiiiihhihihiiiiiihghf`ceebdddddbbcccccccccb_bbbbbccaa_caT_T`bbaaW[_aGW]acccca
+@FCC2HFRACXX:7:1106:15104:10717#TGACCAAT/1
+CCCCCTTGGTGCGGATCATGGCGGCGCCCTCACGGACCCTCCGGAGCGCCTCACCAAGGTCACGG
++
+_b_eeeeegggcgfghiifffdafdagdgaddaccbbaabaaUWQRV__aTW_a^baacGRYYTZ
+@FCC2HFRACXX:7:1106:4831:14328#TGACCAAT/1
+CGCGCATCGGCCACTTCGTCGAGGCGCAGATCCTCGAGGCCGTCGGCGTCGACTACGTCGACGAGAGCGAGGTCCTCACACCCGCC
++
+bb_eeeeegggggiihhifhifhihfhdhfffhhiiaggdbcbcac_\[accL[ac^Q^_VV[[ZTZ^]LZZEJY]``^bbacT[_
+@FCC2HFRACXX:7:1106:15276:33344#TGACCAAT/1
+CCTTAGGGTCGTTGAGGTTGATGCCGACCATGGCCTCCCCGAGTCCGGCGCTGACGTCGGCGAGGATGGTAGGGTCGCTGTAGTGGGTGACAGCCTGAAC
++
+bbbeeeeeeggggifiiegfhhihiifhifhhhhhihiihgfffhhigccaaccc_TZ_caaZacT``aSYG[_]^[X]aT]_]^aaHTWY_b`^X[^Y`
+@FCC2HFRACXX:7:1106:19852:34786#TGACCAAT/1
+CCCGGCCTCCCCCTTGGTGCGGATCATGGCGGCGCCCTCACGGACCCTCCGGAGCGCCTCACCAAGGT
++
+bbbeeeeeggfggiiiighiiihiiiiihiiiegecc^`aa^accccbaaT_Xaac_a_a`aa``GWS
+@FCC2HFRACXX:7:1106:14122:44676#TGACCAAT/1
+CCTCTCTTTTGGTTTCCTCGATGCGGGTGCCTTCCGTCCCCCCTATCTATCCTCTCGGGAAGGTTCAGGTTCTCTCTCTCCAGTCAGGGGAGGGAGAAGA
++
+bbbeeeeegggfgiiiiiiiiiiiiiifgfhfhffhhihhiiifgfeeeeeeeddcacc_accbb`bbc__bcccbccccc_bcc_bcccR]aaOXXW`b
+@FCC2HFRACXX:7:1106:6845:47466#TGACCAAT/1
+CCCGCATCGCGGAGGAAGCCGGCGCCTGCGCCGTCATGGCGCTGGAGCGCGTCCCCGCCGACATCCGCTCGCAGGGCGGCGTGGCGCGGATGTCCGACCC
++
+bbbeeeeegggggiifhgfiiihfgihfggeecaccccccccccccccccccccccaccaaaccbbZaacaccaca_acca_cccX][_TX]Y]b^___a
+@FCC2HFRACXX:7:1106:10406:50210#TGACCAAT/1
+CCGACCATGGCCTCCCCGAGTCCGGCGCTGACGTCGGCGAGGATGGGAGGGTCGCTGTAGTGGGGGACAGCCTGAACGATGGCACGCGCGCGG
++
+_bbeeeeeggeggiiiiiPd^ddffe_c_ffghefgfabW^^U^bcQRW[aQXTUUXO_`]`aaLV_a^[`^[`XY]`XGQX^X]]_[XXX[_
+@FCC2HFRACXX:7:1106:17718:58447#TGACCAAT/1
+CGCCTCACCAAGGTCACGGCAGCCACAGACGAAGGGGACACGGAAGTTGTGCTTGTTGATGTGGTGCGCGTCGTCGGCGGG
++
+_bbeeeccegfgedfghdfdghhhbeda_ghfgbghh`ghhhh_bd^d_Z^VZ_]`b]GZ`b]`Z^_\_cXZaX_acaacc
+@FCC2HFRACXX:7:1106:9381:61139#TGACCAAT/1
+GGCAGCCACAGACGAAGGGGACACGGAAGTTGTGCTTGTTGATGTGGTGCGCGTCGTCGGCGGG
++
+bbbeeeeegggggihiiiiighihiifgheffefhhiihhhbghffd_eggddabaacacaV_a
+@FCC2HFRACXX:7:1106:18984:61293#TGACCAAT/1
+ATGGCGGCGCCCTCACGGACCCTCCGGAGCGCCTCACCAAGGTCACGGCAGCCACAGAC
++
+___eeeeee_ggcgfhgihhiifhifha`a`abW_bac`aacY`bbW[TT[V^WX[WJQ
+@FCC2HFRACXX:7:1106:12518:64250#TGACCAAT/1
+GCCTTCCCGAGTCCGGCGCTGACGTCGGCGAGGATGGTAGGGTCGCTGTAGTGGGGG
++
+bbbeeeeefggcghhhiiifdheheghihf\`a[bbcU]_aa]`aacc[`bccacET
+@FCC2HFRACXX:7:1106:4033:64596#TGACCAAT/1
+CGCGCCGTCACCATCCCCGTCATGGCCAAAGCGCGCATCGGCCACTTCGTCGAGGCGCAGATCCTCGAGGCCGTCGGCGTCGACTACGTCGACGAGAGCG
++
+bbbeeeeegfggfiiiiiihiiiiiiihihhdfhihiiigggeecdcd\baccacccccccbcccccVWZaccac[_aaX_[__W[b[[^]aaaRTX[^_
+@FCC2HFRACXX:7:1106:5545:68939#TGACCAAT/1
+CCTCCACGATGTTGCCGGTCCCGGCCTCCCCCTTGGTGCGGATCATGGCGGCGCCCTCACGGACCCTCCGGAGCGC
++
+___eeeeeegfgghiihhe``efhhifhdfhffcefbffgaddd_cceZ]____aaTW[`^[VV_a[^_ETXTEOT
+@FCC2HFRACXX:7:1106:13935:76457#TGACCAAT/1
+GTCGAGGCGCAGATCCTCGAGGCCGTCGGCGTCGACTACGTCGACGAGAGCGAGGTCCTCACACCCGCCGACGACGCGCACCACATCAACAAGCACAACT
++
+bbbeeeeegggeghiiiihiiiiihghhfaa`cbccaccc^acccaT_W_^TTXaV^bbb`ac`accaccZa_ETX_OT[_c[``]_bcbY^^^^bbccb
+@FCC2HFRACXX:7:1106:10384:82155#TGACCAAT/1
+TGGGGATGGTGACGGCGCGCTTGATGTCGCGGATGAGGCCCGGGGCG
++
+___ccc`ceYb^ceYeeg_effcggfffffFWSYN^cd\``_^^FHT
+@FCC2HFRACXX:7:1106:12148:94474#TGACCAAT/1
+TGCGCGTCGTCGGCGGGTGTGAGGACCTCGCTCTCGTCGACGTAGTCGACGCCGACGGCCTCGAGGATCTGCGCCTCGACGA
++
+_bbeeeceggggghhhhLXX\_bb_`aR]\^[^[_Z^a_VaUVa[W_a[_aacWLX_WT[TWV[a_ZXX`YGTO]OTTT[_O
+@FCC2HFRACXX:7:1107:12148:13865#TGACCAAT/1
+CTCCGGAGCGCCTCACCAAGGTCACGGCAGCCACAGACGAAGGGGACACGGAAGTTGTGCTTGTTGATGTGGTGCGCGTCG
++
+bbbeeeeegggggiihihiiiiiefghH^e^f]eghhffghedde]b\`[^NZ^T]b]^b_bc^`^R]bSS_R[YTTXT[_
+@FCC2HFRACXX:7:1107:12090:73635#TGACCAAT/1
+CCTTGGTGCGGATCATGGCGGCGCCCTCACGGACCCTCCGGAGCGCCTCACCAAGGTCACGGCAGCCAGAGACGAAGGGG
++
+_bbeecdeggfggffffhfcdfegghSbM\`dN\]bdcc^aW^\_TYWW_aab^[^RYbbZaaaaW`W[GW[^QT[Q[_a
+@FCC2HFRACXX:7:1107:5346:89535#TGACCAAT/1
+GTCACCATCCCCGTCATGGCCAAAGCGCGCATCGGCCACTTCGTCGAGGCGCAGATCCTCGAGGCCGTCGGCGTCGACTACGTCGACGAGAGCGAGGTCC
++
+bbbeeeeegfgggfhhhhihiiiihfhifehhhdfhhihiiihgfgdebaaacacccccbcaZacccac_acaOTOX][XJ[``aT]_aEOX`XT[aOX`
+@FCC2HFRACXX:7:1109:6882:8797#TGACCAAT/1
+GCGGGGTCATCATGGACGTCGTCACCCCGGAACAAGCCCGCATCGCGGAGGAAGCCGGCGCCTGCGCCGTCATGGCGCTGGAGCGCGTCCCC
++
+___eeeceggggfd_ffhiffghhiiihfhffggggdgeab`aaccccccW^a`accaccZaa_a_T]aTX[`Yb`a_aa_[^b]_TTT[aa
+@FCC2HFRACXX:7:1110:1464:36301#TGACCAAT/1
+CTCCGGAGCGCCTCACCAAGGTCACGGCAGCCACAGACGAAGGGGACACGGAAGGTGTGCTTGTTGGTGTGGTGCGCGTCGTCGGCGGGGG
++
+bbbeeeeegggfgihiiihiihhdhehffgfgfhhiiihfheggeW`^abaW^aGY`]^`bbcabWGW[WO[R[STT_T[aX^acacacXa
+@FCC2HFRACXX:7:1113:17227:7535#TGACCAAT/1
+CGTTGAGGTTGATGCCGACCATGGCCTCCCCGAGTCCGGCGCTGACGTCGGCGAGGATGGTAGGGTCGCTGTAGTGGGTGA
++
+aa_eeeeeegggghifhfhiiiiihihhiiiihhdgfdghiggeeeccacc^aT[aV]]^GR]^cX]aaZ_a_`GGQ^OTP
+@FCC2HFRACXX:7:1113:2047:83280#TGACCAAT/1
+CTTCTCTTTTGGTTTCCTCGATGCGGGTGCCTTCCGTCCCCCCTATCTATCCTCTCGGGAAGGTTCAGGTTCTCTCTCTCCAGTCAGGGGAGGG
++
+___eeecdggffeeehhdhfhhiiii`ccghSccdgffhffhigddgbceeccbbbW^_NQ[_TRGYYYTGS]`b_`SY_bY_Y]Y]RPTT[[_
+@FCC2HFRACXX:7:1114:6393:73852#TGACCAAT/1
+GTGACGGCGCGCTTGATGTCGCGGATGAGGCCCGGGTCGG
++
+__beecccefgg^gf_`fc_\_edfghffdP^b[`cRRFR
+@FCC2HFRACXX:7:1114:9800:90016#TGACCAAT/1
+CGCAGATGCTCCGTGGCGGGGGCATCATGGACGTCGTCACCCCGGAACAAGCCCGCATCGCGGAGGAAGCCGGCGCCTGCGCCG
++
+bbbeeeeegggggghihiiiiFXZ^`]]`bY`^UVVV^QW_acV_T_aacb^ac[aLOTV^T_RW]^a^W[TTLTTTOW^_[]O
+@FCC2HFRACXX:7:1115:10995:43390#TGACCAAT/1
+GGCGGGGACGCGCTCCAGCGCCATGACGGCGCAGGCGCCGGCTTCCTCCGCGATGCGGGCTTGTTCCGGGGTGACGACGTCCATGATGACCCC
++
+bbbeeeeegfggfhiicdgedeaccddac\a_accacaa_aUR_^b`]`[_WTXWaaaa[[`Z[b]bLTZ]TXQ_[W[_[]aGS]_RY]Y_aa
+@FCC2HFRACXX:7:1115:3865:85768#TGACCAAT/1
+TTTTTTTTGAAGAACATGTGATTCATTCATTGATTCATCATTCATAAGCCATAAGCAGGGCTGG
++
+Z^^ac_ccb^`S[^X^df[bbg_ab[afghfcg[^aX_c_aegbgbfhhf`ed]S_\bfNP^FT
+@FCC2HFRACXX:7:1116:2899:50522#TGNCCANT/1
+CCGGCGGGGACGCGCTCCAGCGCCATGACGGCGCAGGCGCCGGCTTCCTCCG
++
+__becee]`gg`U^abcc`bb^aca__`b^aaa_ccaaPU_TRYTYWWR]XO
+@FCC2HFRACXX:7:1116:14958:80448#TGNCCANT/1
+TCAGGCTGTCACCCACTACAGCGACCCTACCATCCTCGCCGACGTCAGCGCCGGAC
++
+Z_^cce`ccaeS`a`dghdf_g`YYffXZaef_defhO^`e_[`S^^[b[UX^FKO
+@FCC2HFRACXX:7:1204:5783:11609#TGACCAAT/1
+CTCCGCGATGCGGGCTTGTTCCGGGGGGACGACGTCCATGATGACCCCGCCACGGAGCATCTGCGCGAGGC
++
+_bbee`cefefggfdfgecgbgfh]_FOUX_U_OEO[Wa__b`_bc[^[ac[cccWa_^bbcbbccaTZaa
+@FCC2HFRACXX:7:1208:15273:53625#TGACCAAT/1
+CCTCTCTTTTGGTTTCCTCGATGCGGGTGCCTTCCGTCCCCCCTATCTATCCTCTCGGGAAGGTTCAGGTTCTCTCTCTCCAGTCAGGGGAGG
++
+bbbeeeeegggggiihiihihiiiiii^eggghfhhiihiiiigggeeeeceeddccacX^acR_bbbc]`_bcccbcccc_bccbbbccaca
+@FCC2HFRACXX:7:1208:4746:76848#TGACCAAT/1
+GCCGCGATGCGGGCTTGTTCCGGGGTGACGACGTCCATGATGACCCCGCCACGGAGCATCTGCGCGAGGCCGACCTTGATGGAGAAAGTCGCCGACTTGG
++
+_bbeeeccggcgfhhiY^ffhffhifffefZa]]`^P_bcbb`_`acccc_aaa_ccab`bbbaaZOV_]aa_aaa``bGY]X`bWX_SJ[[^[RT[abb
+@FCC2HFRACXX:7:1210:20434:50036#TGACCAAT/1
+CATTCATTGATTCATCATTCATAAGCCATAAGCAGGGCTGGCAGAGCGACAGACATACAAACCGTTCAACA
++
+\^W`accc^YbcY`ba]dedc_YY`ded]^bdehh_dd]c^caacc`c_YY`dhh`Ybdcb\b`Z^_R^cR
+@FCC2HFRACXX:7:1212:13462:36526#TGACCAAT/1
+CTCCATCAGTAATAACGGGACTGAATCAATGGTAAGAAACAAAGCTATGGGACTATGCAGAACCTCTTTATTTTTGTGTCTTCTTATTAGCTACTGTATT
++
+^[^^ccZaeYQ[beehh]Ueeedb[_IYbYXdPYXYI^a^afd_bXcabeGXXWaaebceW\__cbZc_bZ`d]daac_]_a`U]]]a__GLZ`aaZ]ZT
+@FCC2HFRACXX:7:1214:12805:78804#TGACCAAT/1
+ATCAAATGTTGAACGGTTTGTATGTCTGTCGCTCTGCCAGCCCTGCTTATGGCTTATGAATGATGAATCAATGAATGAATCACATGTTCTTCATCCGGTC
++
+bbbeeeeeggffgiiifgiegfgfgfhghhhiifgihiihiihihihihiihihgdhbfge]cg_`d_bbgd`bgb]acecddbbddd_bb_`c`b_a\\
+@FCC2HFRACXX:7:2110:7919:15273#TGACAATC/1
+CGGGGACGCGCTCCAGCGTCATGACGGCGCAGGCGCCGGCTTCCTCCGCGATGCGGGCTTGTTCCGGGG
++
+aa_eeeeeggggfiiihiYcghihhhidggggeecccccccabcbbcaWXT[WaWaa_aaLJ]Y]LOZa
+@FCC2HFRACXX:7:2113:10117:75866#TGACCATC/1
+CTACTGTATTAAGTTAGACTAGGCTCCCCTGGGTTATCATGTACTCAATCAAAAGTTGAACGGTTTGTATGTCTGTCGCTCTGCCAGCCCTGCTTATGGC
++
+_V^ccdacgcccceffihfigfhiifhdggbfH^bfhfhbghaecdcfghii_OIOX^^bggfT_`cbfZ_ddddg``aa\^`Z`bb^^^^`^bc``]RX
+@FCC2HFRACXX:7:2115:7974:84543#TGACCAAT/1
+CGATGCGCTTAGCATACGCGAACACCT
++
+^PY`cce`cgfggfdf`e_`fg`aeHO
+@FCC2HFRACXX:7:2116:16331:24258#TGNCCANT/1
+TGATGCCGACCATGGCCTCCCCGAGTCCGGCGCTGACGTCGGCGAGGATGGGAGGGTCGCTATAGTGGGTG
++
+___eeceeceggghhiiiiiiifcfZeegf_agdWdbddeecccZ_WO]`^GRWYL[b[_VOOR_]``^EO
+@FCC2HFRACXX:7:2208:20999:62970#TGACCAAT/1
+CGTCGGACGGGAGCGGCGTGGTGGCGGTGTACGGGAACAACG
++
+^^Zccc`YcccYcccc`c_ccU_G\NUFXU]]]^^KRW\^[V
+@FCC2HFRACXX:7:2213:20934:38627#TGACCAAT/1
+GTGCCATCGTTCAGGCTGTCACCCACTACAGCGACCCTACCATCCTCGCCGACGTCAGCGCCGGCC
++
+___ee`cc[aegfdgfc``gbgghfZaRbe]Rbeff`e`dacghghcfhhdWa`]aTZb_aaTVEO
+@FCC2HFRACXX:7:2311:18581:78965#TGACCAAT/1
+CCGTTCAACATTTGATTGAGTACATGATAAAACAGGGGAGCCTAGTCTAACTTAAT
++
+^^_Y`^cW`cZbafSb^`ceJYY^agbeeeeJQbdXHYbaddd_ZcecXc_`_ce[
+@FCC2HFRACXX:7:2314:7565:4236#TGACCAAT/1
+GACCAAGCAGCTGGGCCGCCTCCCTGTTGTGCAGTTCGCGGCCGGGGGTGTGGCTAC
++
+___ccccc^egcc_deee]d_g^ZXYaXSYcbXc[afga``_\a]aWVEOT[^aGY^
+@FCC2HFRACXX:7:2316:14788:22713#TGNCCAAT/1
+CATGTACTCAATCAAATGTTGAACGGTTTGTATGTCTGTCGCTCTGCCAGCCCTGCTTATGGCTTATGAATGATGAATCAATGAATGAATCACATGTTCT
++
+_bbecdeeggcgghhfhhhghfhggicgfhhhefhihigfbgfhhhhfbI^cfghhhhdffhghefhbbgdbgeedd_acaabdcb[`]`bcc`cc`b`b