simple_bioc 0.0.6 → 0.0.7
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- checksums.yaml +4 -4
- data/lib/simple_bioc/bioc_merger.rb +6 -4
- data/lib/simple_bioc/version.rb +1 -1
- data/xml/merge/output.xml +972 -0
- metadata +1 -1
data/xml/merge/output.xml
CHANGED
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@@ -36,6 +36,7 @@
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36
36
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<text>Ndc1p</text>
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</annotation>
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<annotation id="G1">
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+
<infon key="type">Gene</infon>
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<location offset="25" length="5"/>
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<text>Ndc1p</text>
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</annotation>
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@@ -145,66 +146,103 @@
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<text>Finally, we have found that a deletion of POM152, which encodes an abundant but nonessential nucleoporin, suppresses the SPB duplication defect associated with a mutation in the NDC1 gene</text>
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</annotation>
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<annotation id="G2">
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+
<infon key="type">Gene</infon>
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<location offset="234" length="35"/>
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<text>Saccharomyces cerevisiae NDC1 gene</text>
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</annotation>
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<annotation id="G3">
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154
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+
<infon key="score">0.70</infon>
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+
<infon key="GeneID">335236</infon>
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+
<infon key="type">Gene</infon>
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152
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<location offset="428" length="4"/>
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<text>NDC1</text>
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</annotation>
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<annotation id="G4">
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+
<infon key="score">0.73</infon>
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162
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+
<infon key="GeneID">854977</infon>
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163
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+
<infon key="type">Gene</infon>
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<location offset="606" length="5"/>
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<text>Ndc1p</text>
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</annotation>
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<annotation id="G5">
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+
<infon key="score">0.73</infon>
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+
<infon key="GeneID">854977</infon>
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+
<infon key="type">Gene</infon>
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<location offset="734" length="5"/>
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<text>Ndc1p</text>
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</annotation>
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<annotation id="G6">
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+
<infon key="score">0.73</infon>
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176
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+
<infon key="GeneID">852703</infon>
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+
<infon key="type">Gene</infon>
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<location offset="837" length="6"/>
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<text>Nup49p</text>
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</annotation>
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<annotation id="G7">
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+
<infon key="score">0.73</infon>
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+
<infon key="GeneID">854977</infon>
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+
<infon key="type">Gene</infon>
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<location offset="878" length="5"/>
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<text>Ndc1p</text>
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</annotation>
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<annotation id="G8">
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+
<infon key="score">0.73</infon>
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+
<infon key="GeneID">853824</infon>
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+
<infon key="type">Gene</infon>
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<location offset="936" length="6"/>
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<text>Spc42p</text>
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</annotation>
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<annotation id="G9">
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196
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+
<infon key="score">0.73</infon>
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+
<infon key="GeneID">854977</infon>
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+
<infon key="type">Gene</infon>
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<location offset="982" length="5"/>
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<text>Ndc1p</text>
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</annotation>
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<annotation id="G10">
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+
<infon key="score">0.70</infon>
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+
<infon key="GeneID">855159</infon>
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+
<infon key="type">Gene</infon>
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<location offset="1197" length="6"/>
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<text>POM152</text>
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</annotation>
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<annotation id="G11">
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+
<infon key="score">0.70</infon>
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<infon key="GeneID">335236</infon>
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<infon key="type">Gene</infon>
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<location offset="1334" length="9"/>
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<text>NDC1 gene</text>
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</annotation>
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<annotation id="G12">
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<infon key="score">0.73</infon>
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<infon key="GeneID">854977</infon>
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+
<infon key="type">Gene</infon>
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<location offset="1359" length="5"/>
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<text>Ndc1p</text>
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</annotation>
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<annotation id="TEAM_277_0">
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+
<infon key="type">Gene</infon>
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<location offset="260" length="4"/>
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<text>NDC1</text>
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</annotation>
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<annotation id="TEAM_277_1">
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<infon key="type">Gene</infon>
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<location offset="428" length="4"/>
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<text>NDC1</text>
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</annotation>
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<annotation id="TEAM_277_2">
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+
<infon key="type">Gene</infon>
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<location offset="1197" length="6"/>
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<text>POM152</text>
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</annotation>
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<annotation id="TEAM_277_3">
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+
<infon key="type">Gene</infon>
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<location offset="1334" length="4"/>
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<text>NDC1</text>
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</annotation>
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<annotation id="0">
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+
<infon key="type">ExperimentalMethod</infon>
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<infon key="PSIMI">0416</infon>
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<location offset="684" length="160"/>
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<text>Indirect immunofluorescence microscopy shows that Ndc1p displays punctate, nuclear peripheral localization that colocalizes with a known NPC component, Nup49p.</text>
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</annotation>
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@@ -294,22 +332,33 @@
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<text>yeast</text>
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</annotation>
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<annotation id="G13">
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+
<infon key="score">0.73</infon>
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<infon key="GeneID">855159</infon>
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<infon key="type">Gene</infon>
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<location offset="2390" length="7"/>
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<text>Pom152p</text>
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</annotation>
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<annotation id="G14">
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<infon key="score">0.70</infon>
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<infon key="GeneID">855159</infon>
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+
<infon key="type">Gene</infon>
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<location offset="2522" length="6"/>
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<text>POM152</text>
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</annotation>
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<annotation id="G15">
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+
<infon key="score">0.73</infon>
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+
<infon key="GeneID">855159</infon>
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+
<infon key="type">Gene</infon>
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<location offset="2800" length="7"/>
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<text>Pom152p</text>
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</annotation>
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<annotation id="TEAM_277_4">
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+
<infon key="type">Gene</infon>
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<location offset="2522" length="6"/>
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<text>POM152</text>
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</annotation>
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<annotation id="TEAM_277_5">
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+
<infon key="type">Gene</infon>
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<location offset="2626" length="6"/>
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<text>POM152</text>
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</annotation>
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@@ -366,26 +415,38 @@
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<text>Ndc1p</text>
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</annotation>
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<annotation id="G16">
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418
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+
<infon key="score">0.58</infon>
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419
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+
<infon key="GeneID">335236</infon>
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+
<infon key="type">Gene</infon>
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<location offset="3613" length="9"/>
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<text>NDC1 gene</text>
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</annotation>
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<annotation id="G17">
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+
<infon key="type">Gene</infon>
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<location offset="3748" length="6"/>
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<text>ndc1-1</text>
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</annotation>
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<annotation id="G18">
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<infon key="score">0.70</infon>
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<infon key="GeneID">335236</infon>
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<infon key="type">Gene</infon>
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<location offset="4470" length="4"/>
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<text>NDC1</text>
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</annotation>
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<annotation id="G19">
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<infon key="score">0.73</infon>
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<infon key="GeneID">854977</infon>
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<infon key="type">Gene</infon>
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<location offset="4612" length="5"/>
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<text>Ndc1p</text>
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</annotation>
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<annotation id="TEAM_277_6">
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<infon key="type">Gene</infon>
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<location offset="3613" length="4"/>
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<text>NDC1</text>
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</annotation>
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<annotation id="TEAM_277_7">
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<infon key="type">Gene</infon>
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<location offset="4470" length="4"/>
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<text>NDC1</text>
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</annotation>
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@@ -412,18 +473,28 @@
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<text>POM152</text>
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</annotation>
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<annotation id="G20">
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<infon key="score">0.73</infon>
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<infon key="GeneID">854977</infon>
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<infon key="type">Gene</infon>
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<location offset="4730" length="5"/>
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<text>Ndc1p</text>
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</annotation>
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<annotation id="G21">
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<infon key="score">0.70</infon>
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<infon key="GeneID">855159</infon>
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<infon key="type">Gene</infon>
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<location offset="4848" length="6"/>
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<text>POM152</text>
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</annotation>
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<annotation id="G22">
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<infon key="score">0.70</infon>
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<infon key="GeneID">100009194</infon>
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<infon key="type">Gene</infon>
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<location offset="4878" length="3"/>
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<text>SPB</text>
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495
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</annotation>
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<annotation id="TEAM_277_8">
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+
<infon key="type">Gene</infon>
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498
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<location offset="4848" length="6"/>
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<text>POM152</text>
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</annotation>
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@@ -701,182 +772,266 @@
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<text>The ndc1-1, pom152 null strain [HC5-31c(1166); Table I] was constructed by replacing the entire POM152 open reading frame, plus an additional 163 bp after the stop codon, with the HIS3 gene (Baudin et al., 1993)</text>
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</annotation>
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<annotation id="G23">
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<infon key="score">0.70</infon>
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<infon key="GeneID">335236</infon>
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<infon key="type">Gene</infon>
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<location offset="5204" length="4"/>
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<text>NDC1</text>
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</annotation>
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<annotation id="G24">
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<infon key="score">0.70</infon>
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<infon key="GeneID">7502638</infon>
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784
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<infon key="type">Gene</infon>
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<location offset="5314" length="4"/>
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<text>ProA</text>
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</annotation>
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<annotation id="G25">
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<infon key="type">Gene</infon>
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<location offset="5464" length="6"/>
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<text>ndc1-1</text>
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</annotation>
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<annotation id="G26">
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<infon key="score">0.70</infon>
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+
<infon key="GeneID">855159</infon>
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+
<infon key="type">Gene</infon>
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<location offset="5472" length="6"/>
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<text>pom152</text>
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718
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</annotation>
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719
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<annotation id="G27">
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801
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<infon key="score">0.75</infon>
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+
<infon key="GeneID">2539698</infon>
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+
<infon key="type">Gene</infon>
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<location offset="5642" length="9"/>
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<text>HIS3 gene</text>
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</annotation>
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<annotation id="G28">
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808
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<infon key="score">0.70</infon>
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+
<infon key="GeneID">852703</infon>
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+
<infon key="type">Gene</infon>
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<location offset="5704" length="9"/>
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<text>NUP49-GFP</text>
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</annotation>
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<annotation id="G29">
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815
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+
<infon key="type">Gene</infon>
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<location offset="5847" length="7"/>
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<text>nup49-1</text>
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</annotation>
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<annotation id="G30">
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+
<infon key="type">Gene</infon>
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<location offset="5865" length="14"/>
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<text>nup49DeltaGFLG</text>
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</annotation>
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824
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<annotation id="G31">
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<infon key="score">0.70</infon>
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<infon key="GeneID">852703</infon>
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<infon key="type">Gene</infon>
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<location offset="5927" length="9"/>
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<text>NUP49-GFP</text>
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</annotation>
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739
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<annotation id="G32">
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832
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+
<infon key="type">Gene</infon>
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<location offset="5946" length="18"/>
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<text>nup133 null allele</text>
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</annotation>
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<annotation id="G33">
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837
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<infon key="type">Gene</infon>
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838
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<location offset="6101" length="11"/>
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<text>nup133Delta</text>
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</annotation>
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747
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<annotation id="G34">
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842
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<infon key="score">0.75</infon>
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843
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<infon key="GeneID">2539698</infon>
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<infon key="type">Gene</infon>
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<location offset="6114" length="4"/>
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749
846
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<text>HIS3</text>
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750
847
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</annotation>
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751
848
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<annotation id="G35">
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849
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+
<infon key="type">Gene</infon>
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<location offset="6120" length="7"/>
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<text>nup49-1</text>
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</annotation>
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853
|
<annotation id="G36">
|
|
854
|
+
<infon key="type">Gene</infon>
|
|
756
855
|
<location offset="6139" length="14"/>
|
|
757
856
|
<text>nup49DeltaGFLG</text>
|
|
758
857
|
</annotation>
|
|
759
858
|
<annotation id="G37">
|
|
859
|
+
<infon key="type">Gene</infon>
|
|
760
860
|
<location offset="6197" length="10"/>
|
|
761
861
|
<text>NIC96-ProA</text>
|
|
762
862
|
</annotation>
|
|
763
863
|
<annotation id="G38">
|
|
864
|
+
<infon key="score">0.73</infon>
|
|
865
|
+
<infon key="GeneID">853824</infon>
|
|
866
|
+
<infon key="type">Gene</infon>
|
|
764
867
|
<location offset="6329" length="9"/>
|
|
765
868
|
<text>SPC42-GFP</text>
|
|
766
869
|
</annotation>
|
|
767
870
|
<annotation id="G39">
|
|
871
|
+
<infon key="type">Gene</infon>
|
|
768
872
|
<location offset="6355" length="12"/>
|
|
769
873
|
<text>HC23-11d/16a</text>
|
|
770
874
|
</annotation>
|
|
771
875
|
<annotation id="G40">
|
|
876
|
+
<infon key="score">0.70</infon>
|
|
877
|
+
<infon key="GeneID">54411</infon>
|
|
878
|
+
<infon key="type">Gene</infon>
|
|
772
879
|
<location offset="6364" length="3"/>
|
|
773
880
|
<text>16a</text>
|
|
774
881
|
</annotation>
|
|
775
882
|
<annotation id="G41">
|
|
883
|
+
<infon key="type">Gene</infon>
|
|
776
884
|
<location offset="6497" length="10"/>
|
|
777
885
|
<text>spc42Delta</text>
|
|
778
886
|
</annotation>
|
|
779
887
|
<annotation id="G42">
|
|
888
|
+
<infon key="score">0.59</infon>
|
|
889
|
+
<infon key="GeneID">668253</infon>
|
|
890
|
+
<infon key="type">Gene</infon>
|
|
780
891
|
<location offset="6509" length="4"/>
|
|
781
892
|
<text>LEU2</text>
|
|
782
893
|
</annotation>
|
|
783
894
|
<annotation id="G43">
|
|
895
|
+
<infon key="score">0.59</infon>
|
|
896
|
+
<infon key="GeneID">100342170</infon>
|
|
897
|
+
<infon key="type">Gene</infon>
|
|
784
898
|
<location offset="6519" length="4"/>
|
|
785
899
|
<text>TRP1</text>
|
|
786
900
|
</annotation>
|
|
787
901
|
<annotation id="G44">
|
|
902
|
+
<infon key="score">0.73</infon>
|
|
903
|
+
<infon key="GeneID">853824</infon>
|
|
904
|
+
<infon key="type">Gene</infon>
|
|
788
905
|
<location offset="6525" length="9"/>
|
|
789
906
|
<text>SPC42-GFP</text>
|
|
790
907
|
</annotation>
|
|
791
908
|
<annotation id="G45">
|
|
909
|
+
<infon key="score">0.70</infon>
|
|
910
|
+
<infon key="GeneID">335236</infon>
|
|
911
|
+
<infon key="type">Gene</infon>
|
|
792
912
|
<location offset="6649" length="4"/>
|
|
793
913
|
<text>NDC1</text>
|
|
794
914
|
</annotation>
|
|
795
915
|
<annotation id="G46">
|
|
916
|
+
<infon key="score">0.70</infon>
|
|
917
|
+
<infon key="GeneID">335236</infon>
|
|
918
|
+
<infon key="type">Gene</infon>
|
|
796
919
|
<location offset="6667" length="4"/>
|
|
797
920
|
<text>NDC1</text>
|
|
798
921
|
</annotation>
|
|
799
922
|
<annotation id="G47">
|
|
923
|
+
<infon key="type">Gene</infon>
|
|
800
924
|
<location offset="6723" length="9"/>
|
|
801
925
|
<text>ndc1Delta</text>
|
|
802
926
|
</annotation>
|
|
803
927
|
<annotation id="G48">
|
|
928
|
+
<infon key="type">Gene</infon>
|
|
804
929
|
<location offset="6734" length="16"/>
|
|
805
930
|
<text>HIS3 null allele</text>
|
|
806
931
|
</annotation>
|
|
807
932
|
<annotation id="G49">
|
|
933
|
+
<infon key="score">0.70</infon>
|
|
934
|
+
<infon key="GeneID">2539698</infon>
|
|
935
|
+
<infon key="type">Gene</infon>
|
|
808
936
|
<location offset="6823" length="9"/>
|
|
809
937
|
<text>HIS3 gene</text>
|
|
810
938
|
</annotation>
|
|
811
939
|
<annotation id="G50">
|
|
940
|
+
<infon key="type">Gene</infon>
|
|
812
941
|
<location offset="6860" length="9"/>
|
|
813
942
|
<text>ndc1Delta</text>
|
|
814
943
|
</annotation>
|
|
815
944
|
<annotation id="G51">
|
|
945
|
+
<infon key="type">Gene</infon>
|
|
816
946
|
<location offset="6872" length="17"/>
|
|
817
947
|
<text>KANMX null allele</text>
|
|
818
948
|
</annotation>
|
|
819
949
|
<annotation id="G52">
|
|
950
|
+
<infon key="score">0.70</infon>
|
|
951
|
+
<infon key="GeneID">335236</infon>
|
|
952
|
+
<infon key="type">Gene</infon>
|
|
820
953
|
<location offset="6987" length="4"/>
|
|
821
954
|
<text>NDC1</text>
|
|
822
955
|
</annotation>
|
|
823
956
|
<annotation id="G53">
|
|
957
|
+
<infon key="type">Gene</infon>
|
|
824
958
|
<location offset="7034" length="10"/>
|
|
825
959
|
<text>KANMX gene</text>
|
|
826
960
|
</annotation>
|
|
827
961
|
<annotation id="TEAM_277_9">
|
|
962
|
+
<infon key="type">Gene</infon>
|
|
828
963
|
<location offset="5204" length="4"/>
|
|
829
964
|
<text>NDC1</text>
|
|
830
965
|
</annotation>
|
|
831
966
|
<annotation id="TEAM_277_10">
|
|
967
|
+
<infon key="type">Gene</infon>
|
|
832
968
|
<location offset="5280" length="32"/>
|
|
833
969
|
<text>IgG binding domains of protein A</text>
|
|
834
970
|
</annotation>
|
|
835
971
|
<annotation id="TEAM_277_11">
|
|
972
|
+
<infon key="type">Gene</infon>
|
|
836
973
|
<location offset="5557" length="6"/>
|
|
837
974
|
<text>POM152</text>
|
|
838
975
|
</annotation>
|
|
839
976
|
<annotation id="TEAM_277_12">
|
|
977
|
+
<infon key="type">Gene</infon>
|
|
978
|
+
<infon key="GeneID">393202</infon>
|
|
840
979
|
<location offset="5642" length="4"/>
|
|
841
980
|
<text>HIS3</text>
|
|
842
981
|
</annotation>
|
|
843
982
|
<annotation id="TEAM_277_13">
|
|
983
|
+
<infon key="type">Gene</infon>
|
|
984
|
+
<infon key="GeneID">393202</infon>
|
|
844
985
|
<location offset="6114" length="4"/>
|
|
845
986
|
<text>HIS3</text>
|
|
846
987
|
</annotation>
|
|
847
988
|
<annotation id="TEAM_277_14">
|
|
989
|
+
<infon key="type">Gene</infon>
|
|
990
|
+
<infon key="GeneID">13745</infon>
|
|
848
991
|
<location offset="6129" length="4"/>
|
|
849
992
|
<text>URA3</text>
|
|
850
993
|
</annotation>
|
|
851
994
|
<annotation id="TEAM_277_15">
|
|
995
|
+
<infon key="type">Gene</infon>
|
|
996
|
+
<infon key="GeneID">9980</infon>
|
|
852
997
|
<location offset="6509" length="4"/>
|
|
853
998
|
<text>LEU2</text>
|
|
854
999
|
</annotation>
|
|
855
1000
|
<annotation id="TEAM_277_16">
|
|
1001
|
+
<infon key="type">Gene</infon>
|
|
856
1002
|
<location offset="6519" length="4"/>
|
|
857
1003
|
<text>TRP1</text>
|
|
858
1004
|
</annotation>
|
|
859
1005
|
<annotation id="TEAM_277_17">
|
|
1006
|
+
<infon key="type">Gene</infon>
|
|
860
1007
|
<location offset="6649" length="4"/>
|
|
861
1008
|
<text>NDC1</text>
|
|
862
1009
|
</annotation>
|
|
863
1010
|
<annotation id="TEAM_277_18">
|
|
1011
|
+
<infon key="type">Gene</infon>
|
|
864
1012
|
<location offset="6667" length="4"/>
|
|
865
1013
|
<text>NDC1</text>
|
|
866
1014
|
</annotation>
|
|
867
1015
|
<annotation id="TEAM_277_19">
|
|
1016
|
+
<infon key="type">Gene</infon>
|
|
1017
|
+
<infon key="GeneID">393202</infon>
|
|
868
1018
|
<location offset="6734" length="4"/>
|
|
869
1019
|
<text>HIS3</text>
|
|
870
1020
|
</annotation>
|
|
871
1021
|
<annotation id="TEAM_277_20">
|
|
1022
|
+
<infon key="type">Gene</infon>
|
|
1023
|
+
<infon key="GeneID">393202</infon>
|
|
872
1024
|
<location offset="6823" length="4"/>
|
|
873
1025
|
<text>HIS3</text>
|
|
874
1026
|
</annotation>
|
|
875
1027
|
<annotation id="TEAM_277_21">
|
|
1028
|
+
<infon key="type">Gene</infon>
|
|
876
1029
|
<location offset="6987" length="4"/>
|
|
877
1030
|
<text>NDC1</text>
|
|
878
1031
|
</annotation>
|
|
879
1032
|
<annotation id="TEAM_277_22">
|
|
1033
|
+
<infon key="type">Gene</infon>
|
|
1034
|
+
<infon key="GeneID">271781</infon>
|
|
880
1035
|
<location offset="7034" length="5"/>
|
|
881
1036
|
<text>KANMX</text>
|
|
882
1037
|
</annotation>
|
|
@@ -1106,70 +1261,102 @@
|
|
|
1106
1261
|
<text>The pRS315-NDC1-green fluorescent protein (GFP) plasmid was constructed by subcloning a PCR fragment containing GFP amplified from the pyEGFP3 plasmid (Cormack et al., 1997) with XbaI restriction sites into an AvrII linker introduced at the end of NDC1 cloned into the pRS315 vector (Sikorski and Hieter, 1989)</text>
|
|
1107
1262
|
</annotation>
|
|
1108
1263
|
<annotation id="G54">
|
|
1264
|
+
<infon key="score">0.70</infon>
|
|
1265
|
+
<infon key="GeneID">335236</infon>
|
|
1266
|
+
<infon key="type">Gene</infon>
|
|
1109
1267
|
<location offset="7961" length="13"/>
|
|
1110
1268
|
<text>NDC1 fragment</text>
|
|
1111
1269
|
</annotation>
|
|
1112
1270
|
<annotation id="G55">
|
|
1271
|
+
<infon key="score">0.70</infon>
|
|
1272
|
+
<infon key="GeneID">55811</infon>
|
|
1273
|
+
<infon key="type">Gene</infon>
|
|
1113
1274
|
<location offset="8006" length="10"/>
|
|
1114
1275
|
<text>SacI sites</text>
|
|
1115
1276
|
</annotation>
|
|
1116
1277
|
<annotation id="G56">
|
|
1278
|
+
<infon key="type">Gene</infon>
|
|
1117
1279
|
<location offset="8171" length="4"/>
|
|
1118
1280
|
<text>URA3</text>
|
|
1119
1281
|
</annotation>
|
|
1120
1282
|
<annotation id="G57">
|
|
1283
|
+
<infon key="score">0.70</infon>
|
|
1284
|
+
<infon key="GeneID">33986</infon>
|
|
1285
|
+
<infon key="type">Gene</infon>
|
|
1121
1286
|
<location offset="8180" length="4"/>
|
|
1122
1287
|
<text>ADE3</text>
|
|
1123
1288
|
</annotation>
|
|
1124
1289
|
<annotation id="G58">
|
|
1290
|
+
<infon key="type">Gene</infon>
|
|
1125
1291
|
<location offset="8346" length="11"/>
|
|
1126
1292
|
<text>BglII sites</text>
|
|
1127
1293
|
</annotation>
|
|
1128
1294
|
<annotation id="G59">
|
|
1295
|
+
<infon key="type">Gene</infon>
|
|
1129
1296
|
<location offset="8386" length="10"/>
|
|
1130
1297
|
<text>BamHI site</text>
|
|
1131
1298
|
</annotation>
|
|
1132
1299
|
<annotation id="G60">
|
|
1300
|
+
<infon key="type">Gene</infon>
|
|
1133
1301
|
<location offset="8435" length="13"/>
|
|
1134
1302
|
<text>GAL1 promoter</text>
|
|
1135
1303
|
</annotation>
|
|
1136
1304
|
<annotation id="G61">
|
|
1305
|
+
<infon key="type">Gene</infon>
|
|
1137
1306
|
<location offset="8482" length="37"/>
|
|
1138
1307
|
<text>pRS315-NDC1-green fluorescent protein</text>
|
|
1139
1308
|
</annotation>
|
|
1140
1309
|
<annotation id="G62">
|
|
1310
|
+
<infon key="score">0.73</infon>
|
|
1311
|
+
<infon key="GeneID">7011691</infon>
|
|
1312
|
+
<infon key="type">Gene</infon>
|
|
1141
1313
|
<location offset="8521" length="3"/>
|
|
1142
1314
|
<text>GFP</text>
|
|
1143
1315
|
</annotation>
|
|
1144
1316
|
<annotation id="G63">
|
|
1317
|
+
<infon key="score">0.73</infon>
|
|
1318
|
+
<infon key="GeneID">7011691</infon>
|
|
1319
|
+
<infon key="type">Gene</infon>
|
|
1145
1320
|
<location offset="8591" length="3"/>
|
|
1146
1321
|
<text>GFP</text>
|
|
1147
1322
|
</annotation>
|
|
1148
1323
|
<annotation id="G64">
|
|
1324
|
+
<infon key="type">Gene</infon>
|
|
1149
1325
|
<location offset="8659" length="23"/>
|
|
1150
1326
|
<text>XbaI restriction sites</text>
|
|
1151
1327
|
</annotation>
|
|
1152
1328
|
<annotation id="G65">
|
|
1329
|
+
<infon key="score">0.70</infon>
|
|
1330
|
+
<infon key="GeneID">335236</infon>
|
|
1331
|
+
<infon key="type">Gene</infon>
|
|
1153
1332
|
<location offset="8729" length="4"/>
|
|
1154
1333
|
<text>NDC1</text>
|
|
1155
1334
|
</annotation>
|
|
1156
1335
|
<annotation id="TEAM_277_23">
|
|
1336
|
+
<infon key="type">Gene</infon>
|
|
1157
1337
|
<location offset="7961" length="4"/>
|
|
1158
1338
|
<text>NDC1</text>
|
|
1159
1339
|
</annotation>
|
|
1160
1340
|
<annotation id="TEAM_277_24">
|
|
1341
|
+
<infon key="type">Gene</infon>
|
|
1342
|
+
<infon key="GeneID">13745</infon>
|
|
1161
1343
|
<location offset="8171" length="4"/>
|
|
1162
1344
|
<text>URA3</text>
|
|
1163
1345
|
</annotation>
|
|
1164
1346
|
<annotation id="TEAM_277_25">
|
|
1347
|
+
<infon key="type">Gene</infon>
|
|
1348
|
+
<infon key="GeneID">11840</infon>
|
|
1165
1349
|
<location offset="8435" length="4"/>
|
|
1166
1350
|
<text>GAL1</text>
|
|
1167
1351
|
</annotation>
|
|
1168
1352
|
<annotation id="TEAM_277_26">
|
|
1353
|
+
<infon key="type">Gene</infon>
|
|
1169
1354
|
<location offset="8729" length="4"/>
|
|
1170
1355
|
<text>NDC1</text>
|
|
1171
1356
|
</annotation>
|
|
1172
1357
|
<annotation id="TEAM_277_27">
|
|
1358
|
+
<infon key="type">Gene</infon>
|
|
1359
|
+
<infon key="GeneID">12205</infon>
|
|
1173
1360
|
<location offset="8751" length="6"/>
|
|
1174
1361
|
<text>pRS315</text>
|
|
1175
1362
|
</annotation>
|
|
@@ -1220,38 +1407,54 @@
|
|
|
1220
1407
|
<text>Nup57p</text>
|
|
1221
1408
|
</annotation>
|
|
1222
1409
|
<annotation id="G66">
|
|
1410
|
+
<infon key="type">Gene</infon>
|
|
1223
1411
|
<location offset="8895" length="9"/>
|
|
1224
1412
|
<text>Kilmartin</text>
|
|
1225
1413
|
</annotation>
|
|
1226
1414
|
<annotation id="G67">
|
|
1415
|
+
<infon key="type">Gene</infon>
|
|
1227
1416
|
<location offset="8921" length="9"/>
|
|
1228
1417
|
<text>Kilmartin</text>
|
|
1229
1418
|
</annotation>
|
|
1230
1419
|
<annotation id="G68">
|
|
1420
|
+
<infon key="score">0.73</infon>
|
|
1421
|
+
<infon key="GeneID">855159</infon>
|
|
1422
|
+
<infon key="type">Gene</infon>
|
|
1231
1423
|
<location offset="9315" length="7"/>
|
|
1232
1424
|
<text>Pom152p</text>
|
|
1233
1425
|
</annotation>
|
|
1234
1426
|
<annotation id="G69">
|
|
1427
|
+
<infon key="type">Gene</infon>
|
|
1235
1428
|
<location offset="9422" length="9"/>
|
|
1236
1429
|
<text>mAb 118C3</text>
|
|
1237
1430
|
</annotation>
|
|
1238
1431
|
<annotation id="G70">
|
|
1432
|
+
<infon key="score">0.73</infon>
|
|
1433
|
+
<infon key="GeneID">851957</infon>
|
|
1434
|
+
<infon key="type">Gene</infon>
|
|
1239
1435
|
<location offset="9511" length="7"/>
|
|
1240
1436
|
<text>Spc110p</text>
|
|
1241
1437
|
</annotation>
|
|
1242
1438
|
<annotation id="G71">
|
|
1439
|
+
<infon key="type">Gene</infon>
|
|
1243
1440
|
<location offset="9648" length="9"/>
|
|
1244
1441
|
<text>Kilmartin</text>
|
|
1245
1442
|
</annotation>
|
|
1246
1443
|
<annotation id="G72">
|
|
1444
|
+
<infon key="score">0.73</infon>
|
|
1445
|
+
<infon key="GeneID">853016</infon>
|
|
1446
|
+
<infon key="type">Gene</infon>
|
|
1247
1447
|
<location offset="9666" length="6"/>
|
|
1248
1448
|
<text>Nup57p</text>
|
|
1249
1449
|
</annotation>
|
|
1250
1450
|
<annotation id="TEAM_277_28">
|
|
1451
|
+
<infon key="type">Gene</infon>
|
|
1251
1452
|
<location offset="9422" length="9"/>
|
|
1252
1453
|
<text>mAb 118C3</text>
|
|
1253
1454
|
</annotation>
|
|
1254
1455
|
<annotation id="TEAM_277_29">
|
|
1456
|
+
<infon key="type">Gene</infon>
|
|
1457
|
+
<infon key="GeneID">683083</infon>
|
|
1255
1458
|
<location offset="9705" length="7"/>
|
|
1256
1459
|
<text>mAb 414</text>
|
|
1257
1460
|
</annotation>
|
|
@@ -1307,6 +1510,8 @@
|
|
|
1307
1510
|
<text>Tween 20</text>
|
|
1308
1511
|
</annotation>
|
|
1309
1512
|
<annotation id="1">
|
|
1513
|
+
<infon key="type">ExperimentalMethod</infon>
|
|
1514
|
+
<infon key="PSIMI">0416</infon>
|
|
1310
1515
|
<location offset="9849" length="341"/>
|
|
1311
1516
|
<text>Buffer solutions used for immunofluorescence (IF) microscopy include PBSA (10 mg/ml NaCl, 0.2 mg/ml KCl, 1.43 mg/ml KH2PO4), solution A (1.2 M sorbitol, 100 mM KPO4, pH 7.5), spheroplasting solution (solution A containing 4.83 mug/ml Zymolyase 100T, 0.1 M beta-mercaptoethanol), and blocker (PBSA containing 10 mg/ml BSA, 0.1% Tween 20).</text>
|
|
1312
1517
|
</annotation>
|
|
@@ -1388,18 +1593,25 @@
|
|
|
1388
1593
|
<text>rabbit</text>
|
|
1389
1594
|
</annotation>
|
|
1390
1595
|
<annotation id="G73">
|
|
1596
|
+
<infon key="score">0.70</infon>
|
|
1597
|
+
<infon key="GeneID">7502638</infon>
|
|
1598
|
+
<infon key="type">Gene</infon>
|
|
1391
1599
|
<location offset="10262" length="4"/>
|
|
1392
1600
|
<text>ProA</text>
|
|
1393
1601
|
</annotation>
|
|
1394
1602
|
<annotation id="G74">
|
|
1603
|
+
<infon key="type">Gene</infon>
|
|
1395
1604
|
<location offset="11140" length="43"/>
|
|
1396
1605
|
<text>Texas red-conjugated goat anti-rabbit IgGs</text>
|
|
1397
1606
|
</annotation>
|
|
1398
1607
|
<annotation id="TEAM_277_30">
|
|
1608
|
+
<infon key="type">Gene</infon>
|
|
1399
1609
|
<location offset="10239" length="19"/>
|
|
1400
1610
|
<text>IgG binding domains</text>
|
|
1401
1611
|
</annotation>
|
|
1402
1612
|
<annotation id="TEAM_277_31">
|
|
1613
|
+
<infon key="type">Gene</infon>
|
|
1614
|
+
<infon key="GeneID">237249</infon>
|
|
1403
1615
|
<location offset="10262" length="4"/>
|
|
1404
1616
|
<text>ProA</text>
|
|
1405
1617
|
</annotation>
|
|
@@ -1409,6 +1621,8 @@
|
|
|
1409
1621
|
<offset>11465</offset>
|
|
1410
1622
|
<text>Standard fluorescence microscopy was carried out using a Zeiss fluorescence microscope equipped with an Empix CCD camera. Images were captured using MetaMorph imaging software (Universal Imaging, West Chester, PA). Deconvolution microscopy was carried out using a Leica DMRXA/RF4/V automated universal microscope equipped with a Cooke SensiCam high performance digital camera. Images were acquired and deconvolved using the Slidebook software package (Intelligent Imaging Innovations, Denver, CO).</text>
|
|
1411
1623
|
<annotation id="2">
|
|
1624
|
+
<infon key="type">ExperimentalMethod</infon>
|
|
1625
|
+
<infon key="PSIMI">0416</infon>
|
|
1412
1626
|
<location offset="11465" length="121"/>
|
|
1413
1627
|
<text>Standard fluorescence microscopy was carried out using a Zeiss fluorescence microscope equipped with an Empix CCD camera.</text>
|
|
1414
1628
|
</annotation>
|
|
@@ -1608,10 +1822,12 @@
|
|
|
1608
1822
|
<text>yeast</text>
|
|
1609
1823
|
</annotation>
|
|
1610
1824
|
<annotation id="G75">
|
|
1825
|
+
<infon key="type">Gene</infon>
|
|
1611
1826
|
<location offset="13631" length="12"/>
|
|
1612
1827
|
<text>HC10-42a/42d</text>
|
|
1613
1828
|
</annotation>
|
|
1614
1829
|
<annotation id="G76">
|
|
1830
|
+
<infon key="type">Gene</infon>
|
|
1615
1831
|
<location offset="13640" length="3"/>
|
|
1616
1832
|
<text>42d</text>
|
|
1617
1833
|
</annotation>
|
|
@@ -1632,6 +1848,9 @@
|
|
|
1632
1848
|
<text>Ndc1p</text>
|
|
1633
1849
|
</annotation>
|
|
1634
1850
|
<annotation id="G77">
|
|
1851
|
+
<infon key="score">0.73</infon>
|
|
1852
|
+
<infon key="GeneID">854977</infon>
|
|
1853
|
+
<infon key="type">Gene</infon>
|
|
1635
1854
|
<location offset="14332" length="5"/>
|
|
1636
1855
|
<text>Ndc1p</text>
|
|
1637
1856
|
</annotation>
|
|
@@ -1677,10 +1896,16 @@
|
|
|
1677
1896
|
<text>Ndc1p</text>
|
|
1678
1897
|
</annotation>
|
|
1679
1898
|
<annotation id="G78">
|
|
1899
|
+
<infon key="score">0.73</infon>
|
|
1900
|
+
<infon key="GeneID">854977</infon>
|
|
1901
|
+
<infon key="type">Gene</infon>
|
|
1680
1902
|
<location offset="14709" length="5"/>
|
|
1681
1903
|
<text>Ndc1p</text>
|
|
1682
1904
|
</annotation>
|
|
1683
1905
|
<annotation id="G79">
|
|
1906
|
+
<infon key="score">0.73</infon>
|
|
1907
|
+
<infon key="GeneID">854977</infon>
|
|
1908
|
+
<infon key="type">Gene</infon>
|
|
1684
1909
|
<location offset="14875" length="5"/>
|
|
1685
1910
|
<text>Ndc1p</text>
|
|
1686
1911
|
</annotation>
|
|
@@ -1744,62 +1969,91 @@
|
|
|
1744
1969
|
<text>NDC1</text>
|
|
1745
1970
|
</annotation>
|
|
1746
1971
|
<annotation id="G80">
|
|
1972
|
+
<infon key="score">0.73</infon>
|
|
1973
|
+
<infon key="GeneID">854977</infon>
|
|
1974
|
+
<infon key="type">Gene</infon>
|
|
1747
1975
|
<location offset="14961" length="5"/>
|
|
1748
1976
|
<text>Ndc1p</text>
|
|
1749
1977
|
</annotation>
|
|
1750
1978
|
<annotation id="G81">
|
|
1979
|
+
<infon key="score">0.70</infon>
|
|
1980
|
+
<infon key="GeneID">335236</infon>
|
|
1981
|
+
<infon key="type">Gene</infon>
|
|
1751
1982
|
<location offset="15034" length="4"/>
|
|
1752
1983
|
<text>NDC1</text>
|
|
1753
1984
|
</annotation>
|
|
1754
1985
|
<annotation id="G82">
|
|
1986
|
+
<infon key="score">0.70</infon>
|
|
1987
|
+
<infon key="GeneID">7502638</infon>
|
|
1988
|
+
<infon key="type">Gene</infon>
|
|
1755
1989
|
<location offset="15109" length="4"/>
|
|
1756
1990
|
<text>ProA</text>
|
|
1757
1991
|
</annotation>
|
|
1758
1992
|
<annotation id="G83">
|
|
1993
|
+
<infon key="score">0.70</infon>
|
|
1994
|
+
<infon key="GeneID">335236</infon>
|
|
1995
|
+
<infon key="type">Gene</infon>
|
|
1759
1996
|
<location offset="15158" length="4"/>
|
|
1760
1997
|
<text>NDC1</text>
|
|
1761
1998
|
</annotation>
|
|
1762
1999
|
<annotation id="G84">
|
|
2000
|
+
<infon key="score">0.70</infon>
|
|
2001
|
+
<infon key="GeneID">335236</infon>
|
|
2002
|
+
<infon key="type">Gene</infon>
|
|
1763
2003
|
<location offset="15224" length="4"/>
|
|
1764
2004
|
<text>NDC1</text>
|
|
1765
2005
|
</annotation>
|
|
1766
2006
|
<annotation id="G85">
|
|
2007
|
+
<infon key="type">Gene</infon>
|
|
1767
2008
|
<location offset="15264" length="13"/>
|
|
1768
2009
|
<text>NDC1 promoter</text>
|
|
1769
2010
|
</annotation>
|
|
1770
2011
|
<annotation id="G86">
|
|
2012
|
+
<infon key="type">Gene</infon>
|
|
1771
2013
|
<location offset="15398" length="10"/>
|
|
1772
2014
|
<text>Ndc1p-ProA</text>
|
|
1773
2015
|
</annotation>
|
|
1774
2016
|
<annotation id="G87">
|
|
2017
|
+
<infon key="score">0.70</infon>
|
|
2018
|
+
<infon key="GeneID">335236</infon>
|
|
2019
|
+
<infon key="type">Gene</infon>
|
|
1775
2020
|
<location offset="15644" length="4"/>
|
|
1776
2021
|
<text>NDC1</text>
|
|
1777
2022
|
</annotation>
|
|
1778
2023
|
<annotation id="G88">
|
|
2024
|
+
<infon key="score">0.70</infon>
|
|
2025
|
+
<infon key="GeneID">100327265</infon>
|
|
2026
|
+
<infon key="type">Gene</infon>
|
|
1779
2027
|
<location offset="15685" length="5"/>
|
|
1780
2028
|
<text>c-myc</text>
|
|
1781
2029
|
</annotation>
|
|
1782
2030
|
<annotation id="TEAM_277_32">
|
|
2031
|
+
<infon key="type">Gene</infon>
|
|
1783
2032
|
<location offset="15034" length="4"/>
|
|
1784
2033
|
<text>NDC1</text>
|
|
1785
2034
|
</annotation>
|
|
1786
2035
|
<annotation id="TEAM_277_33">
|
|
2036
|
+
<infon key="type">Gene</infon>
|
|
1787
2037
|
<location offset="15085" length="28"/>
|
|
1788
2038
|
<text>IgG binding domains of ProA</text>
|
|
1789
2039
|
</annotation>
|
|
1790
2040
|
<annotation id="TEAM_277_34">
|
|
2041
|
+
<infon key="type">Gene</infon>
|
|
1791
2042
|
<location offset="15158" length="4"/>
|
|
1792
2043
|
<text>NDC1</text>
|
|
1793
2044
|
</annotation>
|
|
1794
2045
|
<annotation id="TEAM_277_35">
|
|
2046
|
+
<infon key="type">Gene</infon>
|
|
1795
2047
|
<location offset="15224" length="4"/>
|
|
1796
2048
|
<text>NDC1</text>
|
|
1797
2049
|
</annotation>
|
|
1798
2050
|
<annotation id="TEAM_277_36">
|
|
2051
|
+
<infon key="type">Gene</infon>
|
|
1799
2052
|
<location offset="15264" length="4"/>
|
|
1800
2053
|
<text>NDC1</text>
|
|
1801
2054
|
</annotation>
|
|
1802
2055
|
<annotation id="TEAM_277_37">
|
|
2056
|
+
<infon key="type">Gene</infon>
|
|
1803
2057
|
<location offset="15644" length="4"/>
|
|
1804
2058
|
<text>NDC1</text>
|
|
1805
2059
|
</annotation>
|
|
@@ -1833,50 +2087,81 @@
|
|
|
1833
2087
|
<text>nup133</text>
|
|
1834
2088
|
</annotation>
|
|
1835
2089
|
<annotation id="G89">
|
|
2090
|
+
<infon key="score">0.73</infon>
|
|
2091
|
+
<infon key="GeneID">852703</infon>
|
|
2092
|
+
<infon key="type">Gene</infon>
|
|
1836
2093
|
<location offset="15815" length="6"/>
|
|
1837
2094
|
<text>Nup49p</text>
|
|
1838
2095
|
</annotation>
|
|
1839
2096
|
<annotation id="G90">
|
|
2097
|
+
<infon key="score">0.70</infon>
|
|
2098
|
+
<infon key="GeneID">852703</infon>
|
|
2099
|
+
<infon key="type">Gene</infon>
|
|
1840
2100
|
<location offset="15907" length="5"/>
|
|
1841
2101
|
<text>NUP49</text>
|
|
1842
2102
|
</annotation>
|
|
1843
2103
|
<annotation id="G91">
|
|
2104
|
+
<infon key="score">0.73</infon>
|
|
2105
|
+
<infon key="GeneID">852703</infon>
|
|
2106
|
+
<infon key="type">Gene</infon>
|
|
1844
2107
|
<location offset="16080" length="10"/>
|
|
1845
2108
|
<text>Nup49p-GFP</text>
|
|
1846
2109
|
</annotation>
|
|
1847
2110
|
<annotation id="G92">
|
|
2111
|
+
<infon key="score">0.73</infon>
|
|
2112
|
+
<infon key="GeneID">852703</infon>
|
|
2113
|
+
<infon key="type">Gene</infon>
|
|
1848
2114
|
<location offset="16187" length="10"/>
|
|
1849
2115
|
<text>Nup49p-GFP</text>
|
|
1850
2116
|
</annotation>
|
|
1851
2117
|
<annotation id="G93">
|
|
2118
|
+
<infon key="score">0.70</infon>
|
|
2119
|
+
<infon key="GeneID">7502638</infon>
|
|
2120
|
+
<infon key="type">Gene</infon>
|
|
1852
2121
|
<location offset="16211" length="12"/>
|
|
1853
2122
|
<text>ProA epitope</text>
|
|
1854
2123
|
</annotation>
|
|
1855
2124
|
<annotation id="G94">
|
|
2125
|
+
<infon key="score">0.73</infon>
|
|
2126
|
+
<infon key="GeneID">854977</infon>
|
|
2127
|
+
<infon key="type">Gene</infon>
|
|
1856
2128
|
<location offset="16227" length="5"/>
|
|
1857
2129
|
<text>Ndc1p</text>
|
|
1858
2130
|
</annotation>
|
|
1859
2131
|
<annotation id="G95">
|
|
2132
|
+
<infon key="score">0.58</infon>
|
|
2133
|
+
<infon key="GeneID">406852</infon>
|
|
2134
|
+
<infon key="type">Gene</infon>
|
|
1860
2135
|
<location offset="16349" length="6"/>
|
|
1861
2136
|
<text>nup133</text>
|
|
1862
2137
|
</annotation>
|
|
1863
2138
|
<annotation id="G96">
|
|
2139
|
+
<infon key="score">0.73</infon>
|
|
2140
|
+
<infon key="GeneID">852703</infon>
|
|
2141
|
+
<infon key="type">Gene</infon>
|
|
1864
2142
|
<location offset="16520" length="10"/>
|
|
1865
2143
|
<text>Nup49p-GFP</text>
|
|
1866
2144
|
</annotation>
|
|
1867
2145
|
<annotation id="G97">
|
|
2146
|
+
<infon key="type">Gene</infon>
|
|
1868
2147
|
<location offset="16582" length="10"/>
|
|
1869
2148
|
<text>Ndc1p-ProA</text>
|
|
1870
2149
|
</annotation>
|
|
1871
2150
|
<annotation id="G98">
|
|
2151
|
+
<infon key="score">0.73</infon>
|
|
2152
|
+
<infon key="GeneID">852703</infon>
|
|
2153
|
+
<infon key="type">Gene</infon>
|
|
1872
2154
|
<location offset="16759" length="10"/>
|
|
1873
2155
|
<text>Nup49p-GFP</text>
|
|
1874
2156
|
</annotation>
|
|
1875
2157
|
<annotation id="TEAM_277_38">
|
|
2158
|
+
<infon key="type">Gene</infon>
|
|
2159
|
+
<infon key="GeneID">4415</infon>
|
|
1876
2160
|
<location offset="15907" length="5"/>
|
|
1877
2161
|
<text>NUP49</text>
|
|
1878
2162
|
</annotation>
|
|
1879
2163
|
<annotation id="TEAM_277_39">
|
|
2164
|
+
<infon key="type">Gene</infon>
|
|
1880
2165
|
<location offset="16520" length="10"/>
|
|
1881
2166
|
<text>Nup49p-GFP</text>
|
|
1882
2167
|
</annotation>
|
|
@@ -1933,34 +2218,52 @@
|
|
|
1933
2218
|
<text>Pom152p</text>
|
|
1934
2219
|
</annotation>
|
|
1935
2220
|
<annotation id="G99">
|
|
2221
|
+
<infon key="type">Gene</infon>
|
|
1936
2222
|
<location offset="16899" length="10"/>
|
|
1937
2223
|
<text>Ndc1p-ProA</text>
|
|
1938
2224
|
</annotation>
|
|
1939
2225
|
<annotation id="G100">
|
|
2226
|
+
<infon key="score">0.73</infon>
|
|
2227
|
+
<infon key="GeneID">855159</infon>
|
|
2228
|
+
<infon key="type">Gene</infon>
|
|
1940
2229
|
<location offset="16980" length="7"/>
|
|
1941
2230
|
<text>Pom152p</text>
|
|
1942
2231
|
</annotation>
|
|
1943
2232
|
<annotation id="G101">
|
|
2233
|
+
<infon key="score">0.73</infon>
|
|
2234
|
+
<infon key="GeneID">854977</infon>
|
|
2235
|
+
<infon key="type">Gene</infon>
|
|
1944
2236
|
<location offset="17237" length="5"/>
|
|
1945
2237
|
<text>Ndc1p</text>
|
|
1946
2238
|
</annotation>
|
|
1947
2239
|
<annotation id="G102">
|
|
2240
|
+
<infon key="score">0.73</infon>
|
|
2241
|
+
<infon key="GeneID">853016</infon>
|
|
2242
|
+
<infon key="type">Gene</infon>
|
|
1948
2243
|
<location offset="17493" length="6"/>
|
|
1949
2244
|
<text>Nup57p</text>
|
|
1950
2245
|
</annotation>
|
|
1951
2246
|
<annotation id="G103">
|
|
2247
|
+
<infon key="score">0.73</infon>
|
|
2248
|
+
<infon key="GeneID">855159</infon>
|
|
2249
|
+
<infon key="type">Gene</infon>
|
|
1952
2250
|
<location offset="17555" length="7"/>
|
|
1953
2251
|
<text>Pom152p</text>
|
|
1954
2252
|
</annotation>
|
|
1955
2253
|
<annotation id="G104">
|
|
2254
|
+
<infon key="type">Gene</infon>
|
|
1956
2255
|
<location offset="17884" length="10"/>
|
|
1957
2256
|
<text>Ndc1p-ProA</text>
|
|
1958
2257
|
</annotation>
|
|
1959
2258
|
<annotation id="TEAM_277_40">
|
|
2259
|
+
<infon key="type">Gene</infon>
|
|
2260
|
+
<infon key="GeneID">203327</infon>
|
|
1960
2261
|
<location offset="16868" length="3"/>
|
|
1961
2262
|
<text>NEs</text>
|
|
1962
2263
|
</annotation>
|
|
1963
2264
|
<annotation id="TEAM_277_41">
|
|
2265
|
+
<infon key="type">Gene</infon>
|
|
2266
|
+
<infon key="GeneID">4415</infon>
|
|
1964
2267
|
<location offset="17493" length="6"/>
|
|
1965
2268
|
<text>Nup57p</text>
|
|
1966
2269
|
</annotation>
|
|
@@ -1976,10 +2279,15 @@
|
|
|
1976
2279
|
<text>Ndc1p</text>
|
|
1977
2280
|
</annotation>
|
|
1978
2281
|
<annotation id="G105">
|
|
2282
|
+
<infon key="score">0.73</infon>
|
|
2283
|
+
<infon key="GeneID">854977</infon>
|
|
2284
|
+
<infon key="type">Gene</infon>
|
|
1979
2285
|
<location offset="17937" length="5"/>
|
|
1980
2286
|
<text>Ndc1p</text>
|
|
1981
2287
|
</annotation>
|
|
1982
2288
|
<annotation id="TEAM_277_42">
|
|
2289
|
+
<infon key="type">Gene</infon>
|
|
2290
|
+
<infon key="GeneID">10277</infon>
|
|
1983
2291
|
<location offset="17961" length="4"/>
|
|
1984
2292
|
<text>SPBs</text>
|
|
1985
2293
|
</annotation>
|
|
@@ -2007,26 +2315,36 @@
|
|
|
2007
2315
|
<text>Because NDC1 function is required for proper SPB duplication, we tested whether Ndc1p also localizes to SPBs</text>
|
|
2008
2316
|
</annotation>
|
|
2009
2317
|
<annotation id="G106">
|
|
2318
|
+
<infon key="score">0.70</infon>
|
|
2319
|
+
<infon key="GeneID">335236</infon>
|
|
2320
|
+
<infon key="type">Gene</infon>
|
|
2010
2321
|
<location offset="17974" length="4"/>
|
|
2011
2322
|
<text>NDC1</text>
|
|
2012
2323
|
</annotation>
|
|
2013
2324
|
<annotation id="G107">
|
|
2325
|
+
<infon key="score">0.73</infon>
|
|
2326
|
+
<infon key="GeneID">854977</infon>
|
|
2327
|
+
<infon key="type">Gene</infon>
|
|
2014
2328
|
<location offset="18046" length="5"/>
|
|
2015
2329
|
<text>Ndc1p</text>
|
|
2016
2330
|
</annotation>
|
|
2017
2331
|
<annotation id="G108">
|
|
2332
|
+
<infon key="type">Gene</infon>
|
|
2018
2333
|
<location offset="18378" length="10"/>
|
|
2019
2334
|
<text>Ndc1p-ProA</text>
|
|
2020
2335
|
</annotation>
|
|
2021
2336
|
<annotation id="G109">
|
|
2337
|
+
<infon key="type">Gene</infon>
|
|
2022
2338
|
<location offset="18399" length="9"/>
|
|
2023
2339
|
<text>Kilmartin</text>
|
|
2024
2340
|
</annotation>
|
|
2025
2341
|
<annotation id="G110">
|
|
2342
|
+
<infon key="type">Gene</infon>
|
|
2026
2343
|
<location offset="18467" length="10"/>
|
|
2027
2344
|
<text>Ndc1p-ProA</text>
|
|
2028
2345
|
</annotation>
|
|
2029
2346
|
<annotation id="TEAM_277_43">
|
|
2347
|
+
<infon key="type">Gene</infon>
|
|
2030
2348
|
<location offset="17974" length="4"/>
|
|
2031
2349
|
<text>NDC1</text>
|
|
2032
2350
|
</annotation>
|
|
@@ -2094,42 +2412,65 @@
|
|
|
2094
2412
|
<text>Nic96p</text>
|
|
2095
2413
|
</annotation>
|
|
2096
2414
|
<annotation id="G111">
|
|
2415
|
+
<infon key="score">0.73</infon>
|
|
2416
|
+
<infon key="GeneID">854977</infon>
|
|
2417
|
+
<infon key="type">Gene</infon>
|
|
2097
2418
|
<location offset="18895" length="5"/>
|
|
2098
2419
|
<text>Ndc1p</text>
|
|
2099
2420
|
</annotation>
|
|
2100
2421
|
<annotation id="G112">
|
|
2422
|
+
<infon key="score">0.73</infon>
|
|
2423
|
+
<infon key="GeneID">853824</infon>
|
|
2424
|
+
<infon key="type">Gene</infon>
|
|
2101
2425
|
<location offset="19144" length="9"/>
|
|
2102
2426
|
<text>SPC42-GFP</text>
|
|
2103
2427
|
</annotation>
|
|
2104
2428
|
<annotation id="G113">
|
|
2429
|
+
<infon key="score">0.73</infon>
|
|
2430
|
+
<infon key="GeneID">853824</infon>
|
|
2431
|
+
<infon key="type">Gene</infon>
|
|
2105
2432
|
<location offset="19177" length="5"/>
|
|
2106
2433
|
<text>SPC42</text>
|
|
2107
2434
|
</annotation>
|
|
2108
2435
|
<annotation id="G114">
|
|
2436
|
+
<infon key="type">Gene</infon>
|
|
2109
2437
|
<location offset="19309" length="9"/>
|
|
2110
2438
|
<text>Kilmartin</text>
|
|
2111
2439
|
</annotation>
|
|
2112
2440
|
<annotation id="G115">
|
|
2441
|
+
<infon key="type">Gene</infon>
|
|
2113
2442
|
<location offset="19340" length="9"/>
|
|
2114
2443
|
<text>Kilmartin</text>
|
|
2115
2444
|
</annotation>
|
|
2116
2445
|
<annotation id="G116">
|
|
2446
|
+
<infon key="score">0.73</infon>
|
|
2447
|
+
<infon key="GeneID">853824</infon>
|
|
2448
|
+
<infon key="type">Gene</infon>
|
|
2117
2449
|
<location offset="19446" length="9"/>
|
|
2118
2450
|
<text>SPC42-GFP</text>
|
|
2119
2451
|
</annotation>
|
|
2120
2452
|
<annotation id="G117">
|
|
2453
|
+
<infon key="score">0.73</infon>
|
|
2454
|
+
<infon key="GeneID">850552</infon>
|
|
2455
|
+
<infon key="type">Gene</infon>
|
|
2121
2456
|
<location offset="19558" length="6"/>
|
|
2122
2457
|
<text>Nic96p</text>
|
|
2123
2458
|
</annotation>
|
|
2124
2459
|
<annotation id="TEAM_277_44">
|
|
2460
|
+
<infon key="type">Gene</infon>
|
|
2461
|
+
<infon key="GeneID">10855</infon>
|
|
2125
2462
|
<location offset="19177" length="5"/>
|
|
2126
2463
|
<text>SPC42</text>
|
|
2127
2464
|
</annotation>
|
|
2128
2465
|
<annotation id="TEAM_277_45">
|
|
2466
|
+
<infon key="type">Gene</infon>
|
|
2467
|
+
<infon key="GeneID">10277</infon>
|
|
2129
2468
|
<location offset="19691" length="4"/>
|
|
2130
2469
|
<text>SPBs</text>
|
|
2131
2470
|
</annotation>
|
|
2132
2471
|
<annotation id="3">
|
|
2472
|
+
<infon key="type">ExperimentalMethod</infon>
|
|
2473
|
+
<infon key="PSIMI">0416</infon>
|
|
2133
2474
|
<location offset="18781" length="311"/>
|
|
2134
2475
|
<text>Therefore, we used whole cell indirect IF deconvolution microscopy to test whether any of the distinct spots of Ndc1p staining colocalize with signal from a known SPB component. This high-resolution fluorescence microscopy technique could potentially resolve SPB- and NPC-associated signals in the yeast NE.</text>
|
|
2135
2476
|
</annotation>
|
|
@@ -2163,34 +2504,54 @@
|
|
|
2163
2504
|
<text>Both Ndc1p and Nic96p contained the ProA epitope tag, allowing us to use the same antibodies to detect their localization</text>
|
|
2164
2505
|
</annotation>
|
|
2165
2506
|
<annotation id="G118">
|
|
2507
|
+
<infon key="type">Gene</infon>
|
|
2166
2508
|
<location offset="19947" length="10"/>
|
|
2167
2509
|
<text>Ndc1p-ProA</text>
|
|
2168
2510
|
</annotation>
|
|
2169
2511
|
<annotation id="G119">
|
|
2512
|
+
<infon key="score">0.73</infon>
|
|
2513
|
+
<infon key="GeneID">853824</infon>
|
|
2514
|
+
<infon key="type">Gene</infon>
|
|
2170
2515
|
<location offset="19989" length="10"/>
|
|
2171
2516
|
<text>Spc42p-GFP</text>
|
|
2172
2517
|
</annotation>
|
|
2173
2518
|
<annotation id="G120">
|
|
2519
|
+
<infon key="type">Gene</infon>
|
|
2174
2520
|
<location offset="20071" length="11"/>
|
|
2175
2521
|
<text>Nic96p-ProA</text>
|
|
2176
2522
|
</annotation>
|
|
2177
2523
|
<annotation id="G121">
|
|
2524
|
+
<infon key="score">0.73</infon>
|
|
2525
|
+
<infon key="GeneID">853824</infon>
|
|
2526
|
+
<infon key="type">Gene</infon>
|
|
2178
2527
|
<location offset="20128" length="10"/>
|
|
2179
2528
|
<text>Spc42p-GFP</text>
|
|
2180
2529
|
</annotation>
|
|
2181
2530
|
<annotation id="G122">
|
|
2531
|
+
<infon key="score">0.73</infon>
|
|
2532
|
+
<infon key="GeneID">854977</infon>
|
|
2533
|
+
<infon key="type">Gene</infon>
|
|
2182
2534
|
<location offset="20201" length="5"/>
|
|
2183
2535
|
<text>Ndc1p</text>
|
|
2184
2536
|
</annotation>
|
|
2185
2537
|
<annotation id="G123">
|
|
2538
|
+
<infon key="score">0.73</infon>
|
|
2539
|
+
<infon key="GeneID">850552</infon>
|
|
2540
|
+
<infon key="type">Gene</infon>
|
|
2186
2541
|
<location offset="20211" length="6"/>
|
|
2187
2542
|
<text>Nic96p</text>
|
|
2188
2543
|
</annotation>
|
|
2189
2544
|
<annotation id="G124">
|
|
2545
|
+
<infon key="score">0.70</infon>
|
|
2546
|
+
<infon key="GeneID">7502638</infon>
|
|
2547
|
+
<infon key="type">Gene</infon>
|
|
2190
2548
|
<location offset="20233" length="12"/>
|
|
2191
2549
|
<text>ProA epitope</text>
|
|
2192
2550
|
</annotation>
|
|
2193
2551
|
<annotation id="G125">
|
|
2552
|
+
<infon key="score">0.73</infon>
|
|
2553
|
+
<infon key="GeneID">853824</infon>
|
|
2554
|
+
<infon key="type">Gene</infon>
|
|
2194
2555
|
<location offset="20356" length="10"/>
|
|
2195
2556
|
<text>Spc42p-GFP</text>
|
|
2196
2557
|
</annotation>
|
|
@@ -2200,14 +2561,21 @@
|
|
|
2200
2561
|
<offset>20480</offset>
|
|
2201
2562
|
<text>As described earlier, when examining the colocalization of Ndc1p-ProA with Nup49p-GFP in a NPC clustering mutant strain, we occasionally observed either one or two distinct spots of Ndc1p-ProA staining that did not overlap with Nup49p-GFP signal (Fig. 3 C). It is likely that these additional spots of Ndc1p-ProA staining correspond to SPB localization. Previous work demonstrated that the SPB often localizes within NPC clusters (Heath et al., 1995), explaining why we observed extra spots of Ndc1p-ProA staining only occasionally.</text>
|
|
2202
2563
|
<annotation id="G126">
|
|
2564
|
+
<infon key="score">0.73</infon>
|
|
2565
|
+
<infon key="GeneID">852703</infon>
|
|
2566
|
+
<infon key="type">Gene</infon>
|
|
2203
2567
|
<location offset="20556" length="10"/>
|
|
2204
2568
|
<text>Nup49p-GFP</text>
|
|
2205
2569
|
</annotation>
|
|
2206
2570
|
<annotation id="G127">
|
|
2571
|
+
<infon key="type">Gene</infon>
|
|
2207
2572
|
<location offset="20665" length="10"/>
|
|
2208
2573
|
<text>Ndc1p-ProA</text>
|
|
2209
2574
|
</annotation>
|
|
2210
2575
|
<annotation id="G128">
|
|
2576
|
+
<infon key="score">0.73</infon>
|
|
2577
|
+
<infon key="GeneID">852703</infon>
|
|
2578
|
+
<infon key="type">Gene</infon>
|
|
2211
2579
|
<location offset="20712" length="10"/>
|
|
2212
2580
|
<text>Nup49p-GFP</text>
|
|
2213
2581
|
</annotation>
|
|
@@ -2223,30 +2591,47 @@
|
|
|
2223
2591
|
<text>yeast</text>
|
|
2224
2592
|
</annotation>
|
|
2225
2593
|
<annotation id="G129">
|
|
2594
|
+
<infon key="score">0.73</infon>
|
|
2595
|
+
<infon key="GeneID">854977</infon>
|
|
2596
|
+
<infon key="type">Gene</infon>
|
|
2226
2597
|
<location offset="21062" length="9"/>
|
|
2227
2598
|
<text>Ndc1p-GFP</text>
|
|
2228
2599
|
</annotation>
|
|
2229
2600
|
<annotation id="G130">
|
|
2601
|
+
<infon key="score">0.73</infon>
|
|
2602
|
+
<infon key="GeneID">854977</infon>
|
|
2603
|
+
<infon key="type">Gene</infon>
|
|
2230
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|
<location offset="21165" length="9"/>
|
|
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|
<text>Ndc1p-GFP</text>
|
|
2232
2606
|
</annotation>
|
|
2233
2607
|
<annotation id="G131">
|
|
2608
|
+
<infon key="score">0.73</infon>
|
|
2609
|
+
<infon key="GeneID">854977</infon>
|
|
2610
|
+
<infon key="type">Gene</infon>
|
|
2234
2611
|
<location offset="21239" length="9"/>
|
|
2235
2612
|
<text>Ndc1p-GFP</text>
|
|
2236
2613
|
</annotation>
|
|
2237
2614
|
<annotation id="G132">
|
|
2615
|
+
<infon key="score">0.73</infon>
|
|
2616
|
+
<infon key="GeneID">854977</infon>
|
|
2617
|
+
<infon key="type">Gene</infon>
|
|
2238
2618
|
<location offset="21583" length="9"/>
|
|
2239
2619
|
<text>Ndc1p-GFP</text>
|
|
2240
2620
|
</annotation>
|
|
2241
2621
|
<annotation id="G133">
|
|
2622
|
+
<infon key="score">0.73</infon>
|
|
2623
|
+
<infon key="GeneID">854977</infon>
|
|
2624
|
+
<infon key="type">Gene</infon>
|
|
2242
2625
|
<location offset="21975" length="9"/>
|
|
2243
2626
|
<text>Ndc1p-GFP</text>
|
|
2244
2627
|
</annotation>
|
|
2245
2628
|
<annotation id="TEAM_277_46">
|
|
2629
|
+
<infon key="type">Gene</infon>
|
|
2246
2630
|
<location offset="21239" length="9"/>
|
|
2247
2631
|
<text>Ndc1p-GFP</text>
|
|
2248
2632
|
</annotation>
|
|
2249
2633
|
<annotation id="TEAM_277_47">
|
|
2634
|
+
<infon key="type">Gene</infon>
|
|
2250
2635
|
<location offset="21583" length="9"/>
|
|
2251
2636
|
<text>Ndc1p-GFP</text>
|
|
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2637
|
</annotation>
|
|
@@ -2256,14 +2641,20 @@
|
|
|
2256
2641
|
<offset>22047</offset>
|
|
2257
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|
<text>Immunoelectron Microscopy Demonstrates that Ndc1p-GFP Localizes to the Membrane-associated Regions of NPCs and SPBs</text>
|
|
2258
2643
|
<annotation id="G134">
|
|
2644
|
+
<infon key="score">0.73</infon>
|
|
2645
|
+
<infon key="GeneID">854977</infon>
|
|
2646
|
+
<infon key="type">Gene</infon>
|
|
2259
2647
|
<location offset="22091" length="9"/>
|
|
2260
2648
|
<text>Ndc1p-GFP</text>
|
|
2261
2649
|
</annotation>
|
|
2262
2650
|
<annotation id="TEAM_277_48">
|
|
2651
|
+
<infon key="type">Gene</infon>
|
|
2263
2652
|
<location offset="22091" length="9"/>
|
|
2264
2653
|
<text>Ndc1p-GFP</text>
|
|
2265
2654
|
</annotation>
|
|
2266
2655
|
<annotation id="TEAM_277_49">
|
|
2656
|
+
<infon key="type">Gene</infon>
|
|
2657
|
+
<infon key="GeneID">10277</infon>
|
|
2267
2658
|
<location offset="22159" length="4"/>
|
|
2268
2659
|
<text>SPBs</text>
|
|
2269
2660
|
</annotation>
|
|
@@ -2297,18 +2688,28 @@
|
|
|
2297
2688
|
<text>GFP</text>
|
|
2298
2689
|
</annotation>
|
|
2299
2690
|
<annotation id="G135">
|
|
2691
|
+
<infon key="score">0.73</infon>
|
|
2692
|
+
<infon key="GeneID">854977</infon>
|
|
2693
|
+
<infon key="type">Gene</infon>
|
|
2300
2694
|
<location offset="22250" length="5"/>
|
|
2301
2695
|
<text>Ndc1p</text>
|
|
2302
2696
|
</annotation>
|
|
2303
2697
|
<annotation id="G136">
|
|
2698
|
+
<infon key="score">0.73</infon>
|
|
2699
|
+
<infon key="GeneID">854977</infon>
|
|
2700
|
+
<infon key="type">Gene</infon>
|
|
2304
2701
|
<location offset="22374" length="9"/>
|
|
2305
2702
|
<text>Ndc1p-GFP</text>
|
|
2306
2703
|
</annotation>
|
|
2307
2704
|
<annotation id="G137">
|
|
2705
|
+
<infon key="score">0.73</infon>
|
|
2706
|
+
<infon key="GeneID">854977</infon>
|
|
2707
|
+
<infon key="type">Gene</infon>
|
|
2308
2708
|
<location offset="22597" length="9"/>
|
|
2309
2709
|
<text>Ndc1p-GFP</text>
|
|
2310
2710
|
</annotation>
|
|
2311
2711
|
<annotation id="G138">
|
|
2712
|
+
<infon key="type">Gene</infon>
|
|
2312
2713
|
<location offset="22903" length="10"/>
|
|
2313
2714
|
<text>Ndc1p-ProA</text>
|
|
2314
2715
|
</annotation>
|
|
@@ -2318,6 +2719,7 @@
|
|
|
2318
2719
|
<offset>23036</offset>
|
|
2319
2720
|
<text>ndc1-1 Strains Do Not Exhibit Defects in NPC Functions</text>
|
|
2320
2721
|
<annotation id="G139">
|
|
2722
|
+
<infon key="type">Gene</infon>
|
|
2321
2723
|
<location offset="23036" length="14"/>
|
|
2322
2724
|
<text>ndc1-1 Strains</text>
|
|
2323
2725
|
</annotation>
|
|
@@ -2351,34 +2753,51 @@
|
|
|
2351
2753
|
<text>NDC1</text>
|
|
2352
2754
|
</annotation>
|
|
2353
2755
|
<annotation id="G140">
|
|
2756
|
+
<infon key="score">0.73</infon>
|
|
2757
|
+
<infon key="GeneID">854977</infon>
|
|
2758
|
+
<infon key="type">Gene</infon>
|
|
2354
2759
|
<location offset="23102" length="5"/>
|
|
2355
2760
|
<text>Ndc1p</text>
|
|
2356
2761
|
</annotation>
|
|
2357
2762
|
<annotation id="G141">
|
|
2763
|
+
<infon key="score">0.73</infon>
|
|
2764
|
+
<infon key="GeneID">854977</infon>
|
|
2765
|
+
<infon key="type">Gene</infon>
|
|
2358
2766
|
<location offset="23197" length="5"/>
|
|
2359
2767
|
<text>Ndc1p</text>
|
|
2360
2768
|
</annotation>
|
|
2361
2769
|
<annotation id="G142">
|
|
2770
|
+
<infon key="score">0.73</infon>
|
|
2771
|
+
<infon key="GeneID">854977</infon>
|
|
2772
|
+
<infon key="type">Gene</infon>
|
|
2362
2773
|
<location offset="23277" length="5"/>
|
|
2363
2774
|
<text>Ndc1p</text>
|
|
2364
2775
|
</annotation>
|
|
2365
2776
|
<annotation id="G143">
|
|
2777
|
+
<infon key="type">Gene</infon>
|
|
2366
2778
|
<location offset="23421" length="6"/>
|
|
2367
2779
|
<text>ndc1-1</text>
|
|
2368
2780
|
</annotation>
|
|
2369
2781
|
<annotation id="G144">
|
|
2782
|
+
<infon key="score">0.70</infon>
|
|
2783
|
+
<infon key="GeneID">335236</infon>
|
|
2784
|
+
<infon key="type">Gene</infon>
|
|
2370
2785
|
<location offset="23613" length="4"/>
|
|
2371
2786
|
<text>NDC1</text>
|
|
2372
2787
|
</annotation>
|
|
2373
2788
|
<annotation id="G145">
|
|
2789
|
+
<infon key="type">Gene</infon>
|
|
2374
2790
|
<location offset="23764" length="6"/>
|
|
2375
2791
|
<text>ndc1-1</text>
|
|
2376
2792
|
</annotation>
|
|
2377
2793
|
<annotation id="TEAM_277_50">
|
|
2794
|
+
<infon key="type">Gene</infon>
|
|
2795
|
+
<infon key="GeneID">10277</infon>
|
|
2378
2796
|
<location offset="23111" length="4"/>
|
|
2379
2797
|
<text>SPBs</text>
|
|
2380
2798
|
</annotation>
|
|
2381
2799
|
<annotation id="TEAM_277_51">
|
|
2800
|
+
<infon key="type">Gene</infon>
|
|
2382
2801
|
<location offset="23613" length="4"/>
|
|
2383
2802
|
<text>NDC1</text>
|
|
2384
2803
|
</annotation>
|
|
@@ -2429,66 +2848,93 @@
|
|
|
2429
2848
|
<text>Nic96p</text>
|
|
2430
2849
|
</annotation>
|
|
2431
2850
|
<annotation id="G146">
|
|
2851
|
+
<infon key="type">Gene</infon>
|
|
2432
2852
|
<location offset="23854" length="6"/>
|
|
2433
2853
|
<text>ndc1-1</text>
|
|
2434
2854
|
</annotation>
|
|
2435
2855
|
<annotation id="G147">
|
|
2856
|
+
<infon key="type">Gene</infon>
|
|
2436
2857
|
<location offset="23902" length="13"/>
|
|
2437
2858
|
<text>histone H2B1</text>
|
|
2438
2859
|
</annotation>
|
|
2439
2860
|
<annotation id="G148">
|
|
2861
|
+
<infon key="score">0.73</infon>
|
|
2862
|
+
<infon key="GeneID">7011691</infon>
|
|
2863
|
+
<infon key="type">Gene</infon>
|
|
2440
2864
|
<location offset="23964" length="3"/>
|
|
2441
2865
|
<text>GFP</text>
|
|
2442
2866
|
</annotation>
|
|
2443
2867
|
<annotation id="G149">
|
|
2868
|
+
<infon key="type">Gene</infon>
|
|
2444
2869
|
<location offset="24057" length="9"/>
|
|
2445
2870
|
<text>H2B1p-GFP</text>
|
|
2446
2871
|
</annotation>
|
|
2447
2872
|
<annotation id="G150">
|
|
2873
|
+
<infon key="type">Gene</infon>
|
|
2448
2874
|
<location offset="24371" length="27"/>
|
|
2449
2875
|
<text>yeast ribosomal protein L29</text>
|
|
2450
2876
|
</annotation>
|
|
2451
2877
|
<annotation id="G151">
|
|
2878
|
+
<infon key="score">0.70</infon>
|
|
2879
|
+
<infon key="GeneID">12091</infon>
|
|
2880
|
+
<infon key="type">Gene</infon>
|
|
2452
2881
|
<location offset="24408" length="18"/>
|
|
2453
2882
|
<text>beta-galactosidase</text>
|
|
2454
2883
|
</annotation>
|
|
2455
2884
|
<annotation id="G152">
|
|
2885
|
+
<infon key="type">Gene</infon>
|
|
2456
2886
|
<location offset="24477" length="14"/>
|
|
2457
2887
|
<text>GAL10 promoter</text>
|
|
2458
2888
|
</annotation>
|
|
2459
2889
|
<annotation id="G153">
|
|
2890
|
+
<infon key="score">0.70</infon>
|
|
2891
|
+
<infon key="GeneID">12091</infon>
|
|
2892
|
+
<infon key="type">Gene</infon>
|
|
2460
2893
|
<location offset="24592" length="18"/>
|
|
2461
2894
|
<text>beta-galactosidase</text>
|
|
2462
2895
|
</annotation>
|
|
2463
2896
|
<annotation id="G154">
|
|
2897
|
+
<infon key="type">Gene</infon>
|
|
2464
2898
|
<location offset="24642" length="6"/>
|
|
2465
2899
|
<text>ndc1-1</text>
|
|
2466
2900
|
</annotation>
|
|
2467
2901
|
<annotation id="G155">
|
|
2902
|
+
<infon key="type">Gene</infon>
|
|
2468
2903
|
<location offset="24760" length="6"/>
|
|
2469
2904
|
<text>ndc1-1</text>
|
|
2470
2905
|
</annotation>
|
|
2471
2906
|
<annotation id="G156">
|
|
2907
|
+
<infon key="type">Gene</infon>
|
|
2472
2908
|
<location offset="25021" length="6"/>
|
|
2473
2909
|
<text>ndc1-1</text>
|
|
2474
2910
|
</annotation>
|
|
2475
2911
|
<annotation id="G157">
|
|
2912
|
+
<infon key="score">0.73</infon>
|
|
2913
|
+
<infon key="GeneID">850552</infon>
|
|
2914
|
+
<infon key="type">Gene</infon>
|
|
2476
2915
|
<location offset="25042" length="6"/>
|
|
2477
2916
|
<text>Nic96p</text>
|
|
2478
2917
|
</annotation>
|
|
2479
2918
|
<annotation id="TEAM_277_52">
|
|
2919
|
+
<infon key="type">Gene</infon>
|
|
2480
2920
|
<location offset="24057" length="9"/>
|
|
2481
2921
|
<text>H2B1p-GFP</text>
|
|
2482
2922
|
</annotation>
|
|
2483
2923
|
<annotation id="TEAM_277_53">
|
|
2924
|
+
<infon key="type">Gene</infon>
|
|
2925
|
+
<infon key="GeneID">1081679</infon>
|
|
2484
2926
|
<location offset="24408" length="18"/>
|
|
2485
2927
|
<text>beta-galactosidase</text>
|
|
2486
2928
|
</annotation>
|
|
2487
2929
|
<annotation id="TEAM_277_54">
|
|
2930
|
+
<infon key="type">Gene</infon>
|
|
2931
|
+
<infon key="GeneID">119197</infon>
|
|
2488
2932
|
<location offset="24477" length="14"/>
|
|
2489
2933
|
<text>GAL10 promoter</text>
|
|
2490
2934
|
</annotation>
|
|
2491
2935
|
<annotation id="TEAM_277_55">
|
|
2936
|
+
<infon key="type">Gene</infon>
|
|
2937
|
+
<infon key="GeneID">1081679</infon>
|
|
2492
2938
|
<location offset="24592" length="18"/>
|
|
2493
2939
|
<text>beta-galactosidase</text>
|
|
2494
2940
|
</annotation>
|
|
@@ -2538,50 +2984,69 @@
|
|
|
2538
2984
|
<text>yeast</text>
|
|
2539
2985
|
</annotation>
|
|
2540
2986
|
<annotation id="G158">
|
|
2987
|
+
<infon key="type">Gene</infon>
|
|
2541
2988
|
<location offset="25330" length="6"/>
|
|
2542
2989
|
<text>ndc1-1</text>
|
|
2543
2990
|
</annotation>
|
|
2544
2991
|
<annotation id="G159">
|
|
2992
|
+
<infon key="score">0.73</infon>
|
|
2993
|
+
<infon key="GeneID">850552</infon>
|
|
2994
|
+
<infon key="type">Gene</infon>
|
|
2545
2995
|
<location offset="25393" length="6"/>
|
|
2546
2996
|
<text>Nic96p</text>
|
|
2547
2997
|
</annotation>
|
|
2548
2998
|
<annotation id="G160">
|
|
2999
|
+
<infon key="type">Gene</infon>
|
|
2549
3000
|
<location offset="25422" length="6"/>
|
|
2550
3001
|
<text>ndc1-1</text>
|
|
2551
3002
|
</annotation>
|
|
2552
3003
|
<annotation id="G161">
|
|
3004
|
+
<infon key="type">Gene</infon>
|
|
2553
3005
|
<location offset="25450" length="6"/>
|
|
2554
3006
|
<text>ndc1-1</text>
|
|
2555
3007
|
</annotation>
|
|
2556
3008
|
<annotation id="G162">
|
|
3009
|
+
<infon key="type">Gene</infon>
|
|
2557
3010
|
<location offset="25513" length="10"/>
|
|
2558
3011
|
<text>NIC96-ProA</text>
|
|
2559
3012
|
</annotation>
|
|
2560
3013
|
<annotation id="G163">
|
|
3014
|
+
<infon key="score">0.70</infon>
|
|
3015
|
+
<infon key="GeneID">7502638</infon>
|
|
3016
|
+
<infon key="type">Gene</infon>
|
|
2561
3017
|
<location offset="25597" length="4"/>
|
|
2562
3018
|
<text>ProA</text>
|
|
2563
3019
|
</annotation>
|
|
2564
3020
|
<annotation id="G164">
|
|
3021
|
+
<infon key="type">Gene</infon>
|
|
2565
3022
|
<location offset="25638" length="11"/>
|
|
2566
3023
|
<text>Nic96p-ProA</text>
|
|
2567
3024
|
</annotation>
|
|
2568
3025
|
<annotation id="G165">
|
|
3026
|
+
<infon key="score">0.70</infon>
|
|
3027
|
+
<infon key="GeneID">850552</infon>
|
|
3028
|
+
<infon key="type">Gene</infon>
|
|
2569
3029
|
<location offset="25734" length="5"/>
|
|
2570
3030
|
<text>NIC96</text>
|
|
2571
3031
|
</annotation>
|
|
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|
<annotation id="G166">
|
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+
<infon key="type">Gene</infon>
|
|
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|
<location offset="25881" length="10"/>
|
|
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|
<text>NIC96-ProA</text>
|
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|
</annotation>
|
|
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|
<annotation id="G167">
|
|
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+
<infon key="type">Gene</infon>
|
|
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|
<location offset="25923" length="6"/>
|
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|
<text>ndc1-1</text>
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|
</annotation>
|
|
2580
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|
<annotation id="G168">
|
|
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|
+
<infon key="type">Gene</infon>
|
|
2581
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|
<location offset="26151" length="11"/>
|
|
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|
<text>Nic96p-ProA</text>
|
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|
</annotation>
|
|
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|
<annotation id="TEAM_277_56">
|
|
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+
<infon key="type">Gene</infon>
|
|
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|
+
<infon key="GeneID">224119</infon>
|
|
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|
<location offset="25734" length="5"/>
|
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|
<text>NIC96</text>
|
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|
</annotation>
|
|
@@ -2603,26 +3068,35 @@
|
|
|
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|
<text>Wx340</text>
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|
</annotation>
|
|
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|
<annotation id="G169">
|
|
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+
<infon key="type">Gene</infon>
|
|
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|
<location offset="26939" length="15"/>
|
|
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|
<text>tub2-403 allele</text>
|
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|
</annotation>
|
|
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|
<annotation id="G170">
|
|
3076
|
+
<infon key="score">0.58</infon>
|
|
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|
+
<infon key="GeneID">37888</infon>
|
|
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|
+
<infon key="type">Gene</infon>
|
|
2610
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|
<location offset="26987" length="12"/>
|
|
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|
<text>beta-tubulin</text>
|
|
2612
3081
|
</annotation>
|
|
2613
3082
|
<annotation id="G171">
|
|
3083
|
+
<infon key="type">Gene</infon>
|
|
2614
3084
|
<location offset="27053" length="10"/>
|
|
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|
<text>ndc1-1 SPB</text>
|
|
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3086
|
</annotation>
|
|
2617
3087
|
<annotation id="G172">
|
|
3088
|
+
<infon key="type">Gene</infon>
|
|
2618
3089
|
<location offset="27128" length="6"/>
|
|
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|
<text>ndc1-1</text>
|
|
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|
</annotation>
|
|
2621
3092
|
<annotation id="G173">
|
|
3093
|
+
<infon key="type">Gene</infon>
|
|
2622
3094
|
<location offset="27235" length="15"/>
|
|
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3095
|
<text>tub2-403 allele</text>
|
|
2624
3096
|
</annotation>
|
|
2625
3097
|
<annotation id="TEAM_277_57">
|
|
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|
+
<infon key="type">Gene</infon>
|
|
3099
|
+
<infon key="GeneID">130530</infon>
|
|
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|
<location offset="26987" length="12"/>
|
|
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|
<text>beta-tubulin</text>
|
|
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|
</annotation>
|
|
@@ -2632,10 +3106,12 @@
|
|
|
2632
3106
|
<offset>27344</offset>
|
|
2633
3107
|
<text>Samples were prepared as previously described (Winey et al., 1997) to directly count the number of NPCs in three nuclei from each strain after a shift to the nonpermissive temperature. Nuclei from the tub2-403 cells contained 162, 179, and 232 NPCs, and nuclei from the ndc1-1, tub2-403 double mutant cells contained 180, 193, and 209 NPCs. The maximum number of NPCs observed in wild-type cells occurs in early mitosis, with an average of 142 +- 16.4 NPCs (Winey et al., 1997). Our analysis demonstrated that both strains contained more NPCs than observed in wild-type cells at any point in the cell cycle, indicating that NPC assembly continues to occur when these cells are arrested.</text>
|
|
2634
3108
|
<annotation id="G174">
|
|
3109
|
+
<infon key="type">Gene</infon>
|
|
2635
3110
|
<location offset="27618" length="6"/>
|
|
2636
3111
|
<text>ndc1-1</text>
|
|
2637
3112
|
</annotation>
|
|
2638
3113
|
<annotation id="G175">
|
|
3114
|
+
<infon key="type">Gene</infon>
|
|
2639
3115
|
<location offset="27626" length="8"/>
|
|
2640
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|
<text>tub2-403</text>
|
|
2641
3117
|
</annotation>
|
|
@@ -2656,10 +3132,12 @@
|
|
|
2656
3132
|
<text>POM152</text>
|
|
2657
3133
|
</annotation>
|
|
2658
3134
|
<annotation id="G176">
|
|
3135
|
+
<infon key="type">Gene</infon>
|
|
2659
3136
|
<location offset="28077" length="10"/>
|
|
2660
3137
|
<text>ndc1-1 SPB</text>
|
|
2661
3138
|
</annotation>
|
|
2662
3139
|
<annotation id="TEAM_277_58">
|
|
3140
|
+
<infon key="type">Gene</infon>
|
|
2663
3141
|
<location offset="28055" length="6"/>
|
|
2664
3142
|
<text>POM152</text>
|
|
2665
3143
|
</annotation>
|
|
@@ -2736,42 +3214,60 @@
|
|
|
2736
3214
|
<text>To define a potential functional role for Ndc1p in NPCs, we examined genetic interactions between NDC1 and POM152</text>
|
|
2737
3215
|
</annotation>
|
|
2738
3216
|
<annotation id="G177">
|
|
3217
|
+
<infon key="score">0.73</infon>
|
|
3218
|
+
<infon key="GeneID">854977</infon>
|
|
3219
|
+
<infon key="type">Gene</infon>
|
|
2739
3220
|
<location offset="28150" length="5"/>
|
|
2740
3221
|
<text>Ndc1p</text>
|
|
2741
3222
|
</annotation>
|
|
2742
3223
|
<annotation id="G178">
|
|
3224
|
+
<infon key="score">0.70</infon>
|
|
3225
|
+
<infon key="GeneID">335236</infon>
|
|
3226
|
+
<infon key="type">Gene</infon>
|
|
2743
3227
|
<location offset="28207" length="4"/>
|
|
2744
3228
|
<text>NDC1</text>
|
|
2745
3229
|
</annotation>
|
|
2746
3230
|
<annotation id="G179">
|
|
3231
|
+
<infon key="score">0.70</infon>
|
|
3232
|
+
<infon key="GeneID">855159</infon>
|
|
3233
|
+
<infon key="type">Gene</infon>
|
|
2747
3234
|
<location offset="28217" length="6"/>
|
|
2748
3235
|
<text>POM152</text>
|
|
2749
3236
|
</annotation>
|
|
2750
3237
|
<annotation id="G180">
|
|
3238
|
+
<infon key="score">0.70</infon>
|
|
3239
|
+
<infon key="GeneID">855159</infon>
|
|
3240
|
+
<infon key="type">Gene</infon>
|
|
2751
3241
|
<location offset="28400" length="6"/>
|
|
2752
3242
|
<text>POM152</text>
|
|
2753
3243
|
</annotation>
|
|
2754
3244
|
<annotation id="G181">
|
|
3245
|
+
<infon key="type">Gene</infon>
|
|
2755
3246
|
<location offset="28539" length="6"/>
|
|
2756
3247
|
<text>ndc1-1</text>
|
|
2757
3248
|
</annotation>
|
|
2758
3249
|
<annotation id="G182">
|
|
3250
|
+
<infon key="type">Gene</infon>
|
|
2759
3251
|
<location offset="28555" length="18"/>
|
|
2760
3252
|
<text>pom152 null allele</text>
|
|
2761
3253
|
</annotation>
|
|
2762
3254
|
<annotation id="TEAM_277_59">
|
|
3255
|
+
<infon key="type">Gene</infon>
|
|
2763
3256
|
<location offset="28207" length="4"/>
|
|
2764
3257
|
<text>NDC1</text>
|
|
2765
3258
|
</annotation>
|
|
2766
3259
|
<annotation id="TEAM_277_60">
|
|
3260
|
+
<infon key="type">Gene</infon>
|
|
2767
3261
|
<location offset="28217" length="6"/>
|
|
2768
3262
|
<text>POM152</text>
|
|
2769
3263
|
</annotation>
|
|
2770
3264
|
<annotation id="TEAM_277_61">
|
|
3265
|
+
<infon key="type">Gene</infon>
|
|
2771
3266
|
<location offset="28260" length="6"/>
|
|
2772
3267
|
<text>POM152</text>
|
|
2773
3268
|
</annotation>
|
|
2774
3269
|
<annotation id="TEAM_277_62">
|
|
3270
|
+
<infon key="type">Gene</infon>
|
|
2775
3271
|
<location offset="28400" length="6"/>
|
|
2776
3272
|
<text>POM152</text>
|
|
2777
3273
|
</annotation>
|
|
@@ -2842,62 +3338,81 @@
|
|
|
2842
3338
|
<text>ndc1</text>
|
|
2843
3339
|
</annotation>
|
|
2844
3340
|
<annotation id="G183">
|
|
3341
|
+
<infon key="type">Gene</infon>
|
|
2845
3342
|
<location offset="28687" length="18"/>
|
|
2846
3343
|
<text>pom152 null allele</text>
|
|
2847
3344
|
</annotation>
|
|
2848
3345
|
<annotation id="G184">
|
|
3346
|
+
<infon key="type">Gene</infon>
|
|
2849
3347
|
<location offset="28721" length="6"/>
|
|
2850
3348
|
<text>ndc1-1</text>
|
|
2851
3349
|
</annotation>
|
|
2852
3350
|
<annotation id="G185">
|
|
3351
|
+
<infon key="type">Gene</infon>
|
|
2853
3352
|
<location offset="28754" length="6"/>
|
|
2854
3353
|
<text>ndc1-1</text>
|
|
2855
3354
|
</annotation>
|
|
2856
3355
|
<annotation id="G186">
|
|
3356
|
+
<infon key="type">Gene</infon>
|
|
2857
3357
|
<location offset="28785" length="18"/>
|
|
2858
3358
|
<text>pom152 null allele</text>
|
|
2859
3359
|
</annotation>
|
|
2860
3360
|
<annotation id="G187">
|
|
3361
|
+
<infon key="type">Gene</infon>
|
|
2861
3362
|
<location offset="28887" length="6"/>
|
|
2862
3363
|
<text>ndc1-1</text>
|
|
2863
3364
|
</annotation>
|
|
2864
3365
|
<annotation id="G188">
|
|
3366
|
+
<infon key="score">0.70</infon>
|
|
3367
|
+
<infon key="GeneID">855159</infon>
|
|
3368
|
+
<infon key="type">Gene</infon>
|
|
2865
3369
|
<location offset="28958" length="6"/>
|
|
2866
3370
|
<text>POM152</text>
|
|
2867
3371
|
</annotation>
|
|
2868
3372
|
<annotation id="G189">
|
|
3373
|
+
<infon key="score">0.70</infon>
|
|
3374
|
+
<infon key="GeneID">855159</infon>
|
|
3375
|
+
<infon key="type">Gene</infon>
|
|
2869
3376
|
<location offset="29004" length="6"/>
|
|
2870
3377
|
<text>POM152</text>
|
|
2871
3378
|
</annotation>
|
|
2872
3379
|
<annotation id="G190">
|
|
3380
|
+
<infon key="type">Gene</infon>
|
|
2873
3381
|
<location offset="29064" length="6"/>
|
|
2874
3382
|
<text>ndc1-1</text>
|
|
2875
3383
|
</annotation>
|
|
2876
3384
|
<annotation id="G191">
|
|
3385
|
+
<infon key="type">Gene</infon>
|
|
2877
3386
|
<location offset="29174" length="13"/>
|
|
2878
3387
|
<text>ndc1-1 allele</text>
|
|
2879
3388
|
</annotation>
|
|
2880
3389
|
<annotation id="G192">
|
|
3390
|
+
<infon key="type">Gene</infon>
|
|
2881
3391
|
<location offset="29201" length="18"/>
|
|
2882
3392
|
<text>pom152 null allele</text>
|
|
2883
3393
|
</annotation>
|
|
2884
3394
|
<annotation id="G193">
|
|
3395
|
+
<infon key="type">Gene</infon>
|
|
2885
3396
|
<location offset="29270" length="16"/>
|
|
2886
3397
|
<text>ndc1 null allele</text>
|
|
2887
3398
|
</annotation>
|
|
2888
3399
|
<annotation id="TEAM_277_63">
|
|
3400
|
+
<infon key="type">Gene</infon>
|
|
2889
3401
|
<location offset="28785" length="6"/>
|
|
2890
3402
|
<text>pom152</text>
|
|
2891
3403
|
</annotation>
|
|
2892
3404
|
<annotation id="TEAM_277_64">
|
|
3405
|
+
<infon key="type">Gene</infon>
|
|
2893
3406
|
<location offset="28958" length="6"/>
|
|
2894
3407
|
<text>POM152</text>
|
|
2895
3408
|
</annotation>
|
|
2896
3409
|
<annotation id="TEAM_277_65">
|
|
3410
|
+
<infon key="type">Gene</infon>
|
|
2897
3411
|
<location offset="29004" length="6"/>
|
|
2898
3412
|
<text>POM152</text>
|
|
2899
3413
|
</annotation>
|
|
2900
3414
|
<annotation id="TEAM_277_66">
|
|
3415
|
+
<infon key="type">Gene</infon>
|
|
2901
3416
|
<location offset="29270" length="4"/>
|
|
2902
3417
|
<text>ndc1</text>
|
|
2903
3418
|
</annotation>
|
|
@@ -2946,58 +3461,78 @@
|
|
|
2946
3461
|
<text>pom152</text>
|
|
2947
3462
|
</annotation>
|
|
2948
3463
|
<annotation id="G194">
|
|
3464
|
+
<infon key="score">0.70</infon>
|
|
3465
|
+
<infon key="GeneID">335236</infon>
|
|
3466
|
+
<infon key="type">Gene</infon>
|
|
2949
3467
|
<location offset="29308" length="4"/>
|
|
2950
3468
|
<text>NDC1</text>
|
|
2951
3469
|
</annotation>
|
|
2952
3470
|
<annotation id="G195">
|
|
3471
|
+
<infon key="score">0.70</infon>
|
|
3472
|
+
<infon key="GeneID">855159</infon>
|
|
3473
|
+
<infon key="type">Gene</infon>
|
|
2953
3474
|
<location offset="29507" length="6"/>
|
|
2954
3475
|
<text>POM152</text>
|
|
2955
3476
|
</annotation>
|
|
2956
3477
|
<annotation id="G196">
|
|
3478
|
+
<infon key="type">Gene</infon>
|
|
2957
3479
|
<location offset="29530" length="10"/>
|
|
2958
3480
|
<text>ndc1-1 SPB</text>
|
|
2959
3481
|
</annotation>
|
|
2960
3482
|
<annotation id="G197">
|
|
3483
|
+
<infon key="type">Gene</infon>
|
|
2961
3484
|
<location offset="29784" length="6"/>
|
|
2962
3485
|
<text>ndc1-1</text>
|
|
2963
3486
|
</annotation>
|
|
2964
3487
|
<annotation id="G198">
|
|
3488
|
+
<infon key="type">Gene</infon>
|
|
2965
3489
|
<location offset="29939" length="6"/>
|
|
2966
3490
|
<text>ndc1-1</text>
|
|
2967
3491
|
</annotation>
|
|
2968
3492
|
<annotation id="G199">
|
|
3493
|
+
<infon key="type">Gene</infon>
|
|
2969
3494
|
<location offset="30123" length="6"/>
|
|
2970
3495
|
<text>ndc1-1</text>
|
|
2971
3496
|
</annotation>
|
|
2972
3497
|
<annotation id="G200">
|
|
3498
|
+
<infon key="score">0.70</infon>
|
|
3499
|
+
<infon key="GeneID">855159</infon>
|
|
3500
|
+
<infon key="type">Gene</infon>
|
|
2973
3501
|
<location offset="30131" length="6"/>
|
|
2974
3502
|
<text>pom152</text>
|
|
2975
3503
|
</annotation>
|
|
2976
3504
|
<annotation id="G201">
|
|
3505
|
+
<infon key="type">Gene</infon>
|
|
2977
3506
|
<location offset="30186" length="6"/>
|
|
2978
3507
|
<text>ndc1-1</text>
|
|
2979
3508
|
</annotation>
|
|
2980
3509
|
<annotation id="G202">
|
|
3510
|
+
<infon key="type">Gene</infon>
|
|
2981
3511
|
<location offset="30295" length="18"/>
|
|
2982
3512
|
<text>pom152 null allele</text>
|
|
2983
3513
|
</annotation>
|
|
2984
3514
|
<annotation id="G203">
|
|
3515
|
+
<infon key="type">Gene</infon>
|
|
2985
3516
|
<location offset="30329" length="10"/>
|
|
2986
3517
|
<text>ndc1-1 SPB</text>
|
|
2987
3518
|
</annotation>
|
|
2988
3519
|
<annotation id="TEAM_277_67">
|
|
3520
|
+
<infon key="type">Gene</infon>
|
|
2989
3521
|
<location offset="29308" length="4"/>
|
|
2990
3522
|
<text>NDC1</text>
|
|
2991
3523
|
</annotation>
|
|
2992
3524
|
<annotation id="TEAM_277_68">
|
|
3525
|
+
<infon key="type">Gene</infon>
|
|
2993
3526
|
<location offset="29507" length="6"/>
|
|
2994
3527
|
<text>POM152</text>
|
|
2995
3528
|
</annotation>
|
|
2996
3529
|
<annotation id="TEAM_277_69">
|
|
3530
|
+
<infon key="type">Gene</infon>
|
|
2997
3531
|
<location offset="30107" length="9"/>
|
|
2998
3532
|
<text>Fig. 6 B</text>
|
|
2999
3533
|
</annotation>
|
|
3000
3534
|
<annotation id="TEAM_277_70">
|
|
3535
|
+
<infon key="type">Gene</infon>
|
|
3001
3536
|
<location offset="30262" length="8"/>
|
|
3002
3537
|
<text>Fig. 6 B</text>
|
|
3003
3538
|
</annotation>
|
|
@@ -3041,14 +3576,23 @@
|
|
|
3041
3576
|
<text>Ndc1p</text>
|
|
3042
3577
|
</annotation>
|
|
3043
3578
|
<annotation id="G204">
|
|
3579
|
+
<infon key="score">0.73</infon>
|
|
3580
|
+
<infon key="GeneID">854977</infon>
|
|
3581
|
+
<infon key="type">Gene</infon>
|
|
3044
3582
|
<location offset="30737" length="5"/>
|
|
3045
3583
|
<text>Ndc1p</text>
|
|
3046
3584
|
</annotation>
|
|
3047
3585
|
<annotation id="G205">
|
|
3586
|
+
<infon key="score">0.73</infon>
|
|
3587
|
+
<infon key="GeneID">854977</infon>
|
|
3588
|
+
<infon key="type">Gene</infon>
|
|
3048
3589
|
<location offset="30832" length="5"/>
|
|
3049
3590
|
<text>Ndc1p</text>
|
|
3050
3591
|
</annotation>
|
|
3051
3592
|
<annotation id="G206">
|
|
3593
|
+
<infon key="score">0.73</infon>
|
|
3594
|
+
<infon key="GeneID">854977</infon>
|
|
3595
|
+
<infon key="type">Gene</infon>
|
|
3052
3596
|
<location offset="30952" length="5"/>
|
|
3053
3597
|
<text>Ndc1p</text>
|
|
3054
3598
|
</annotation>
|
|
@@ -3076,14 +3620,23 @@
|
|
|
3076
3620
|
<text>Ndc1p</text>
|
|
3077
3621
|
</annotation>
|
|
3078
3622
|
<annotation id="G207">
|
|
3623
|
+
<infon key="score">0.73</infon>
|
|
3624
|
+
<infon key="GeneID">854977</infon>
|
|
3625
|
+
<infon key="type">Gene</infon>
|
|
3079
3626
|
<location offset="31038" length="5"/>
|
|
3080
3627
|
<text>Ndc1p</text>
|
|
3081
3628
|
</annotation>
|
|
3082
3629
|
<annotation id="G208">
|
|
3630
|
+
<infon key="score">0.73</infon>
|
|
3631
|
+
<infon key="GeneID">854977</infon>
|
|
3632
|
+
<infon key="type">Gene</infon>
|
|
3083
3633
|
<location offset="31420" length="5"/>
|
|
3084
3634
|
<text>Ndc1p</text>
|
|
3085
3635
|
</annotation>
|
|
3086
3636
|
<annotation id="G209">
|
|
3637
|
+
<infon key="score">0.73</infon>
|
|
3638
|
+
<infon key="GeneID">854977</infon>
|
|
3639
|
+
<infon key="type">Gene</infon>
|
|
3087
3640
|
<location offset="31642" length="5"/>
|
|
3088
3641
|
<text>Ndc1p</text>
|
|
3089
3642
|
</annotation>
|
|
@@ -3117,26 +3670,39 @@
|
|
|
3117
3670
|
<text>Ndc1p</text>
|
|
3118
3671
|
</annotation>
|
|
3119
3672
|
<annotation id="G210">
|
|
3673
|
+
<infon key="type">Gene</infon>
|
|
3120
3674
|
<location offset="31855" length="10"/>
|
|
3121
3675
|
<text>Ndc1p- GFP</text>
|
|
3122
3676
|
</annotation>
|
|
3123
3677
|
<annotation id="G211">
|
|
3678
|
+
<infon key="score">0.73</infon>
|
|
3679
|
+
<infon key="GeneID">854977</infon>
|
|
3680
|
+
<infon key="type">Gene</infon>
|
|
3124
3681
|
<location offset="31962" length="9"/>
|
|
3125
3682
|
<text>Ndc1p-GFP</text>
|
|
3126
3683
|
</annotation>
|
|
3127
3684
|
<annotation id="G212">
|
|
3685
|
+
<infon key="score">0.73</infon>
|
|
3686
|
+
<infon key="GeneID">854977</infon>
|
|
3687
|
+
<infon key="type">Gene</infon>
|
|
3128
3688
|
<location offset="32151" length="9"/>
|
|
3129
3689
|
<text>Ndc1p-GFP</text>
|
|
3130
3690
|
</annotation>
|
|
3131
3691
|
<annotation id="G213">
|
|
3692
|
+
<infon key="score">0.73</infon>
|
|
3693
|
+
<infon key="GeneID">854977</infon>
|
|
3694
|
+
<infon key="type">Gene</infon>
|
|
3132
3695
|
<location offset="32511" length="5"/>
|
|
3133
3696
|
<text>Ndc1p</text>
|
|
3134
3697
|
</annotation>
|
|
3135
3698
|
<annotation id="TEAM_277_71">
|
|
3699
|
+
<infon key="type">Gene</infon>
|
|
3136
3700
|
<location offset="31962" length="9"/>
|
|
3137
3701
|
<text>Ndc1p-GFP</text>
|
|
3138
3702
|
</annotation>
|
|
3139
3703
|
<annotation id="4">
|
|
3704
|
+
<infon key="type">ExperimentalMethod</infon>
|
|
3705
|
+
<infon key="PSIMI">0040</infon>
|
|
3140
3706
|
<location offset="31755" length="84"/>
|
|
3141
3707
|
<text>However, SPBs and NPCs are most clearly distinguished by using electron microscopy.</text>
|
|
3142
3708
|
</annotation>
|
|
@@ -3158,34 +3724,50 @@
|
|
|
3158
3724
|
<text>NDC1</text>
|
|
3159
3725
|
</annotation>
|
|
3160
3726
|
<annotation id="G214">
|
|
3727
|
+
<infon key="score">0.73</infon>
|
|
3728
|
+
<infon key="GeneID">2542161</infon>
|
|
3729
|
+
<infon key="type">Gene</infon>
|
|
3161
3730
|
<location offset="32613" length="11"/>
|
|
3162
3731
|
<text>cut11+ gene</text>
|
|
3163
3732
|
</annotation>
|
|
3164
3733
|
<annotation id="G215">
|
|
3734
|
+
<infon key="score">0.70</infon>
|
|
3735
|
+
<infon key="GeneID">335236</infon>
|
|
3736
|
+
<infon key="type">Gene</infon>
|
|
3165
3737
|
<location offset="32661" length="4"/>
|
|
3166
3738
|
<text>NDC1</text>
|
|
3167
3739
|
</annotation>
|
|
3168
3740
|
<annotation id="G216">
|
|
3741
|
+
<infon key="type">Gene</infon>
|
|
3169
3742
|
<location offset="32799" length="6"/>
|
|
3170
3743
|
<text>Cut11p</text>
|
|
3171
3744
|
</annotation>
|
|
3172
3745
|
<annotation id="G217">
|
|
3746
|
+
<infon key="score">0.73</infon>
|
|
3747
|
+
<infon key="GeneID">2542161</infon>
|
|
3748
|
+
<infon key="type">Gene</infon>
|
|
3173
3749
|
<location offset="32844" length="6"/>
|
|
3174
3750
|
<text>cut11+</text>
|
|
3175
3751
|
</annotation>
|
|
3176
3752
|
<annotation id="G218">
|
|
3753
|
+
<infon key="score">0.70</infon>
|
|
3754
|
+
<infon key="GeneID">335236</infon>
|
|
3755
|
+
<infon key="type">Gene</infon>
|
|
3177
3756
|
<location offset="32855" length="4"/>
|
|
3178
3757
|
<text>NDC1</text>
|
|
3179
3758
|
</annotation>
|
|
3180
3759
|
<annotation id="G219">
|
|
3760
|
+
<infon key="type">Gene</infon>
|
|
3181
3761
|
<location offset="32944" length="6"/>
|
|
3182
3762
|
<text>Cut11p</text>
|
|
3183
3763
|
</annotation>
|
|
3184
3764
|
<annotation id="TEAM_277_72">
|
|
3765
|
+
<infon key="type">Gene</infon>
|
|
3185
3766
|
<location offset="32661" length="4"/>
|
|
3186
3767
|
<text>NDC1</text>
|
|
3187
3768
|
</annotation>
|
|
3188
3769
|
<annotation id="TEAM_277_73">
|
|
3770
|
+
<infon key="type">Gene</infon>
|
|
3189
3771
|
<location offset="32855" length="4"/>
|
|
3190
3772
|
<text>NDC1</text>
|
|
3191
3773
|
</annotation>
|
|
@@ -3230,50 +3812,73 @@
|
|
|
3230
3812
|
<text>NDC1</text>
|
|
3231
3813
|
</annotation>
|
|
3232
3814
|
<annotation id="G220">
|
|
3815
|
+
<infon key="score">0.73</infon>
|
|
3816
|
+
<infon key="GeneID">854977</infon>
|
|
3817
|
+
<infon key="type">Gene</infon>
|
|
3233
3818
|
<location offset="32999" length="5"/>
|
|
3234
3819
|
<text>Ndc1p</text>
|
|
3235
3820
|
</annotation>
|
|
3236
3821
|
<annotation id="G221">
|
|
3822
|
+
<infon key="type">Gene</infon>
|
|
3237
3823
|
<location offset="33338" length="13"/>
|
|
3238
3824
|
<text>ndc1-1 allele</text>
|
|
3239
3825
|
</annotation>
|
|
3240
3826
|
<annotation id="G222">
|
|
3827
|
+
<infon key="score">0.70</infon>
|
|
3828
|
+
<infon key="GeneID">335236</infon>
|
|
3829
|
+
<infon key="type">Gene</infon>
|
|
3241
3830
|
<location offset="33424" length="4"/>
|
|
3242
3831
|
<text>NDC1</text>
|
|
3243
3832
|
</annotation>
|
|
3244
3833
|
<annotation id="G223">
|
|
3834
|
+
<infon key="score">0.70</infon>
|
|
3835
|
+
<infon key="GeneID">335236</infon>
|
|
3836
|
+
<infon key="type">Gene</infon>
|
|
3245
3837
|
<location offset="33478" length="9"/>
|
|
3246
3838
|
<text>NDC1 gene</text>
|
|
3247
3839
|
</annotation>
|
|
3248
3840
|
<annotation id="G224">
|
|
3841
|
+
<infon key="type">Gene</infon>
|
|
3249
3842
|
<location offset="33510" length="13"/>
|
|
3250
3843
|
<text>ndc1-1 allele</text>
|
|
3251
3844
|
</annotation>
|
|
3252
3845
|
<annotation id="G225">
|
|
3846
|
+
<infon key="score">0.70</infon>
|
|
3847
|
+
<infon key="GeneID">335236</infon>
|
|
3848
|
+
<infon key="type">Gene</infon>
|
|
3253
3849
|
<location offset="33855" length="4"/>
|
|
3254
3850
|
<text>NDC1</text>
|
|
3255
3851
|
</annotation>
|
|
3256
3852
|
<annotation id="G226">
|
|
3853
|
+
<infon key="score">0.70</infon>
|
|
3854
|
+
<infon key="GeneID">335236</infon>
|
|
3855
|
+
<infon key="type">Gene</infon>
|
|
3257
3856
|
<location offset="33879" length="4"/>
|
|
3258
3857
|
<text>NDC1</text>
|
|
3259
3858
|
</annotation>
|
|
3260
3859
|
<annotation id="TEAM_277_74">
|
|
3860
|
+
<infon key="type">Gene</infon>
|
|
3861
|
+
<infon key="GeneID">10277</infon>
|
|
3261
3862
|
<location offset="33012" length="3"/>
|
|
3262
3863
|
<text>SPB</text>
|
|
3263
3864
|
</annotation>
|
|
3264
3865
|
<annotation id="TEAM_277_75">
|
|
3866
|
+
<infon key="type">Gene</infon>
|
|
3265
3867
|
<location offset="33424" length="4"/>
|
|
3266
3868
|
<text>NDC1</text>
|
|
3267
3869
|
</annotation>
|
|
3268
3870
|
<annotation id="TEAM_277_76">
|
|
3871
|
+
<infon key="type">Gene</infon>
|
|
3269
3872
|
<location offset="33478" length="4"/>
|
|
3270
3873
|
<text>NDC1</text>
|
|
3271
3874
|
</annotation>
|
|
3272
3875
|
<annotation id="TEAM_277_77">
|
|
3876
|
+
<infon key="type">Gene</infon>
|
|
3273
3877
|
<location offset="33855" length="4"/>
|
|
3274
3878
|
<text>NDC1</text>
|
|
3275
3879
|
</annotation>
|
|
3276
3880
|
<annotation id="TEAM_277_78">
|
|
3881
|
+
<infon key="type">Gene</infon>
|
|
3277
3882
|
<location offset="33879" length="4"/>
|
|
3278
3883
|
<text>NDC1</text>
|
|
3279
3884
|
</annotation>
|
|
@@ -3406,78 +4011,123 @@
|
|
|
3406
4011
|
<text>Additionally, synthetic lethal interactions have been identified between mutant alleles of NIC96, NUP188, and POM152 (Aitchison et al., 1995b; Nehrbass et al., 1996; Zabel et al., 1996)</text>
|
|
3407
4012
|
</annotation>
|
|
3408
4013
|
<annotation id="G227">
|
|
4014
|
+
<infon key="score">0.70</infon>
|
|
4015
|
+
<infon key="GeneID">850552</infon>
|
|
4016
|
+
<infon key="type">Gene</infon>
|
|
3409
4017
|
<location offset="34100" length="10"/>
|
|
3410
4018
|
<text>NIC96 gene</text>
|
|
3411
4019
|
</annotation>
|
|
3412
4020
|
<annotation id="G228">
|
|
4021
|
+
<infon key="score">0.70</infon>
|
|
4022
|
+
<infon key="GeneID">850552</infon>
|
|
4023
|
+
<infon key="type">Gene</infon>
|
|
3413
4024
|
<location offset="34379" length="5"/>
|
|
3414
4025
|
<text>NIC96</text>
|
|
3415
4026
|
</annotation>
|
|
3416
4027
|
<annotation id="G229">
|
|
4028
|
+
<infon key="score">0.58</infon>
|
|
4029
|
+
<infon key="GeneID">380283</infon>
|
|
4030
|
+
<infon key="type">Gene</infon>
|
|
3417
4031
|
<location offset="34393" length="5"/>
|
|
3418
4032
|
<text>NUP93</text>
|
|
3419
4033
|
</annotation>
|
|
3420
4034
|
<annotation id="G230">
|
|
4035
|
+
<infon key="type">Gene</infon>
|
|
3421
4036
|
<location offset="34464" length="6"/>
|
|
3422
4037
|
<text>Nup93p</text>
|
|
3423
4038
|
</annotation>
|
|
3424
4039
|
<annotation id="G231">
|
|
4040
|
+
<infon key="score">0.73</infon>
|
|
4041
|
+
<infon key="GeneID">850552</infon>
|
|
4042
|
+
<infon key="type">Gene</infon>
|
|
3425
4043
|
<location offset="34590" length="6"/>
|
|
3426
4044
|
<text>Nic96p</text>
|
|
3427
4045
|
</annotation>
|
|
3428
4046
|
<annotation id="G232">
|
|
4047
|
+
<infon key="score">0.73</infon>
|
|
4048
|
+
<infon key="GeneID">853409</infon>
|
|
4049
|
+
<infon key="type">Gene</infon>
|
|
3429
4050
|
<location offset="34692" length="5"/>
|
|
3430
4051
|
<text>Nsp1p</text>
|
|
3431
4052
|
</annotation>
|
|
3432
4053
|
<annotation id="G233">
|
|
4054
|
+
<infon key="score">0.73</infon>
|
|
4055
|
+
<infon key="GeneID">852703</infon>
|
|
4056
|
+
<infon key="type">Gene</infon>
|
|
3433
4057
|
<location offset="34699" length="6"/>
|
|
3434
4058
|
<text>Nup49p</text>
|
|
3435
4059
|
</annotation>
|
|
3436
4060
|
<annotation id="G234">
|
|
4061
|
+
<infon key="score">0.73</infon>
|
|
4062
|
+
<infon key="GeneID">853016</infon>
|
|
4063
|
+
<infon key="type">Gene</infon>
|
|
3437
4064
|
<location offset="34711" length="6"/>
|
|
3438
4065
|
<text>Nup57p</text>
|
|
3439
4066
|
</annotation>
|
|
3440
4067
|
<annotation id="G235">
|
|
4068
|
+
<infon key="score">0.70</infon>
|
|
4069
|
+
<infon key="GeneID">850552</infon>
|
|
4070
|
+
<infon key="type">Gene</infon>
|
|
3441
4071
|
<location offset="34912" length="5"/>
|
|
3442
4072
|
<text>NIC96</text>
|
|
3443
4073
|
</annotation>
|
|
3444
4074
|
<annotation id="G236">
|
|
4075
|
+
<infon key="score">0.58</infon>
|
|
4076
|
+
<infon key="GeneID">100135081</infon>
|
|
4077
|
+
<infon key="type">Gene</infon>
|
|
3445
4078
|
<location offset="34919" length="6"/>
|
|
3446
4079
|
<text>NUP188</text>
|
|
3447
4080
|
</annotation>
|
|
3448
4081
|
<annotation id="TEAM_277_79">
|
|
4082
|
+
<infon key="type">Gene</infon>
|
|
4083
|
+
<infon key="GeneID">224119</infon>
|
|
3449
4084
|
<location offset="34100" length="5"/>
|
|
3450
4085
|
<text>NIC96</text>
|
|
3451
4086
|
</annotation>
|
|
3452
4087
|
<annotation id="TEAM_277_80">
|
|
4088
|
+
<infon key="type">Gene</infon>
|
|
4089
|
+
<infon key="GeneID">224119</infon>
|
|
3453
4090
|
<location offset="34379" length="5"/>
|
|
3454
4091
|
<text>NIC96</text>
|
|
3455
4092
|
</annotation>
|
|
3456
4093
|
<annotation id="TEAM_277_81">
|
|
4094
|
+
<infon key="type">Gene</infon>
|
|
4095
|
+
<infon key="GeneID">4415</infon>
|
|
3457
4096
|
<location offset="34393" length="5"/>
|
|
3458
4097
|
<text>NUP93</text>
|
|
3459
4098
|
</annotation>
|
|
3460
4099
|
<annotation id="TEAM_277_82">
|
|
4100
|
+
<infon key="type">Gene</infon>
|
|
4101
|
+
<infon key="GeneID">4415</infon>
|
|
3461
4102
|
<location offset="34464" length="6"/>
|
|
3462
4103
|
<text>Nup93p</text>
|
|
3463
4104
|
</annotation>
|
|
3464
4105
|
<annotation id="TEAM_277_83">
|
|
4106
|
+
<infon key="type">Gene</infon>
|
|
4107
|
+
<infon key="GeneID">461059</infon>
|
|
3465
4108
|
<location offset="34692" length="5"/>
|
|
3466
4109
|
<text>Nsp1p</text>
|
|
3467
4110
|
</annotation>
|
|
3468
4111
|
<annotation id="TEAM_277_84">
|
|
4112
|
+
<infon key="type">Gene</infon>
|
|
4113
|
+
<infon key="GeneID">4415</infon>
|
|
3469
4114
|
<location offset="34699" length="6"/>
|
|
3470
4115
|
<text>Nup49p</text>
|
|
3471
4116
|
</annotation>
|
|
3472
4117
|
<annotation id="TEAM_277_85">
|
|
4118
|
+
<infon key="type">Gene</infon>
|
|
4119
|
+
<infon key="GeneID">224119</infon>
|
|
3473
4120
|
<location offset="34912" length="5"/>
|
|
3474
4121
|
<text>NIC96</text>
|
|
3475
4122
|
</annotation>
|
|
3476
4123
|
<annotation id="TEAM_277_86">
|
|
4124
|
+
<infon key="type">Gene</infon>
|
|
4125
|
+
<infon key="GeneID">4415</infon>
|
|
3477
4126
|
<location offset="34919" length="6"/>
|
|
3478
4127
|
<text>NUP188</text>
|
|
3479
4128
|
</annotation>
|
|
3480
4129
|
<annotation id="TEAM_277_87">
|
|
4130
|
+
<infon key="type">Gene</infon>
|
|
3481
4131
|
<location offset="34931" length="6"/>
|
|
3482
4132
|
<text>POM152</text>
|
|
3483
4133
|
</annotation>
|
|
@@ -3527,34 +4177,48 @@
|
|
|
3527
4177
|
<text>Because a function for Ndc1p at NPCs has not been determined, we do not know if the suppression of ndc1-1 by the pom152 null allele has any functional consequence for NPCs</text>
|
|
3528
4178
|
</annotation>
|
|
3529
4179
|
<annotation id="G237">
|
|
4180
|
+
<infon key="type">Gene</infon>
|
|
3530
4181
|
<location offset="35084" length="18"/>
|
|
3531
4182
|
<text>pom152 null allele</text>
|
|
3532
4183
|
</annotation>
|
|
3533
4184
|
<annotation id="G238">
|
|
4185
|
+
<infon key="score">0.73</infon>
|
|
4186
|
+
<infon key="GeneID">855159</infon>
|
|
4187
|
+
<infon key="type">Gene</infon>
|
|
3534
4188
|
<location offset="35243" length="7"/>
|
|
3535
4189
|
<text>Pom152p</text>
|
|
3536
4190
|
</annotation>
|
|
3537
4191
|
<annotation id="G239">
|
|
4192
|
+
<infon key="type">Gene</infon>
|
|
3538
4193
|
<location offset="35318" length="10"/>
|
|
3539
4194
|
<text>ndc1-1 SPB</text>
|
|
3540
4195
|
</annotation>
|
|
3541
4196
|
<annotation id="G240">
|
|
4197
|
+
<infon key="type">Gene</infon>
|
|
3542
4198
|
<location offset="35388" length="10"/>
|
|
3543
4199
|
<text>ndc1-1 SPB</text>
|
|
3544
4200
|
</annotation>
|
|
3545
4201
|
<annotation id="G241">
|
|
4202
|
+
<infon key="score">0.73</infon>
|
|
4203
|
+
<infon key="GeneID">854977</infon>
|
|
4204
|
+
<infon key="type">Gene</infon>
|
|
3546
4205
|
<location offset="35518" length="5"/>
|
|
3547
4206
|
<text>Ndc1p</text>
|
|
3548
4207
|
</annotation>
|
|
3549
4208
|
<annotation id="G242">
|
|
4209
|
+
<infon key="type">Gene</infon>
|
|
3550
4210
|
<location offset="35595" length="6"/>
|
|
3551
4211
|
<text>ndc1-1</text>
|
|
3552
4212
|
</annotation>
|
|
3553
4213
|
<annotation id="G243">
|
|
4214
|
+
<infon key="type">Gene</infon>
|
|
3554
4215
|
<location offset="35609" length="18"/>
|
|
3555
4216
|
<text>pom152 null allele</text>
|
|
3556
4217
|
</annotation>
|
|
3557
4218
|
<annotation id="G244">
|
|
4219
|
+
<infon key="score">0.73</infon>
|
|
4220
|
+
<infon key="GeneID">443043</infon>
|
|
4221
|
+
<infon key="type">Gene</infon>
|
|
3558
4222
|
<location offset="35730" length="4"/>
|
|
3559
4223
|
<text>SPBs</text>
|
|
3560
4224
|
</annotation>
|
|
@@ -3648,54 +4312,75 @@
|
|
|
3648
4312
|
<text>The mechanism by which the pom152 null allele suppresses the ndc1-1 SPB duplication defect is not known, but it appears as if wild-type POM152 is antagonistic in ndc1-1 strains</text>
|
|
3649
4313
|
</annotation>
|
|
3650
4314
|
<annotation id="G245">
|
|
4315
|
+
<infon key="type">Gene</infon>
|
|
3651
4316
|
<location offset="35851" length="18"/>
|
|
3652
4317
|
<text>pom152 null allele</text>
|
|
3653
4318
|
</annotation>
|
|
3654
4319
|
<annotation id="G246">
|
|
4320
|
+
<infon key="type">Gene</infon>
|
|
3655
4321
|
<location offset="35885" length="10"/>
|
|
3656
4322
|
<text>ndc1-1 SPB</text>
|
|
3657
4323
|
</annotation>
|
|
3658
4324
|
<annotation id="G247">
|
|
4325
|
+
<infon key="type">Gene</infon>
|
|
3659
4326
|
<location offset="35988" length="6"/>
|
|
3660
4327
|
<text>ndc1-1</text>
|
|
3661
4328
|
</annotation>
|
|
3662
4329
|
<annotation id="G248">
|
|
4330
|
+
<infon key="score">0.73</infon>
|
|
4331
|
+
<infon key="GeneID">855159</infon>
|
|
4332
|
+
<infon key="type">Gene</infon>
|
|
3663
4333
|
<location offset="36083" length="7"/>
|
|
3664
4334
|
<text>Pom152p</text>
|
|
3665
4335
|
</annotation>
|
|
3666
4336
|
<annotation id="G249">
|
|
4337
|
+
<infon key="score">0.70</infon>
|
|
4338
|
+
<infon key="GeneID">855159</infon>
|
|
4339
|
+
<infon key="type">Gene</infon>
|
|
3667
4340
|
<location offset="36172" length="11"/>
|
|
3668
4341
|
<text>POM152 gene</text>
|
|
3669
4342
|
</annotation>
|
|
3670
4343
|
<annotation id="G250">
|
|
4344
|
+
<infon key="score">0.70</infon>
|
|
4345
|
+
<infon key="GeneID">855159</infon>
|
|
4346
|
+
<infon key="type">Gene</infon>
|
|
3671
4347
|
<location offset="36225" length="6"/>
|
|
3672
4348
|
<text>POM152</text>
|
|
3673
4349
|
</annotation>
|
|
3674
4350
|
<annotation id="G251">
|
|
4351
|
+
<infon key="score">0.70</infon>
|
|
4352
|
+
<infon key="GeneID">855159</infon>
|
|
4353
|
+
<infon key="type">Gene</infon>
|
|
3675
4354
|
<location offset="36328" length="6"/>
|
|
3676
4355
|
<text>POM152</text>
|
|
3677
4356
|
</annotation>
|
|
3678
4357
|
<annotation id="TEAM_277_88">
|
|
4358
|
+
<infon key="type">Gene</infon>
|
|
3679
4359
|
<location offset="35851" length="6"/>
|
|
3680
4360
|
<text>pom152</text>
|
|
3681
4361
|
</annotation>
|
|
3682
4362
|
<annotation id="TEAM_277_89">
|
|
4363
|
+
<infon key="type">Gene</infon>
|
|
3683
4364
|
<location offset="35961" length="6"/>
|
|
3684
4365
|
<text>POM152</text>
|
|
3685
4366
|
</annotation>
|
|
3686
4367
|
<annotation id="TEAM_277_90">
|
|
4368
|
+
<infon key="type">Gene</infon>
|
|
3687
4369
|
<location offset="36172" length="6"/>
|
|
3688
4370
|
<text>POM152</text>
|
|
3689
4371
|
</annotation>
|
|
3690
4372
|
<annotation id="TEAM_277_91">
|
|
4373
|
+
<infon key="type">Gene</infon>
|
|
3691
4374
|
<location offset="36225" length="6"/>
|
|
3692
4375
|
<text>POM152</text>
|
|
3693
4376
|
</annotation>
|
|
3694
4377
|
<annotation id="TEAM_277_92">
|
|
4378
|
+
<infon key="type">Gene</infon>
|
|
3695
4379
|
<location offset="36328" length="6"/>
|
|
3696
4380
|
<text>POM152</text>
|
|
3697
4381
|
</annotation>
|
|
3698
4382
|
<annotation id="TEAM_277_93">
|
|
4383
|
+
<infon key="type">Gene</infon>
|
|
3699
4384
|
<location offset="36464" length="6"/>
|
|
3700
4385
|
<text>POM152</text>
|
|
3701
4386
|
</annotation>
|
|
@@ -3747,46 +4432,69 @@
|
|
|
3747
4432
|
<text>It is possible that Ndc1p and Pom152p may directly interact at NPCs, because immunoelectron microscopy shows that these two proteins localize to similar regions of the NPC (Wozniak et al., 1994), and both are membrane proteins</text>
|
|
3748
4433
|
</annotation>
|
|
3749
4434
|
<annotation id="G252">
|
|
4435
|
+
<infon key="score">0.73</infon>
|
|
4436
|
+
<infon key="GeneID">855159</infon>
|
|
4437
|
+
<infon key="type">Gene</infon>
|
|
3750
4438
|
<location offset="36514" length="7"/>
|
|
3751
4439
|
<text>Pom152p</text>
|
|
3752
4440
|
</annotation>
|
|
3753
4441
|
<annotation id="G253">
|
|
4442
|
+
<infon key="type">Gene</infon>
|
|
3754
4443
|
<location offset="36623" length="7"/>
|
|
3755
4444
|
<text>Ndc1-1p</text>
|
|
3756
4445
|
</annotation>
|
|
3757
4446
|
<annotation id="G254">
|
|
4447
|
+
<infon key="type">Gene</infon>
|
|
3758
4448
|
<location offset="36814" length="13"/>
|
|
3759
4449
|
<text>ndc1-1 allele</text>
|
|
3760
4450
|
</annotation>
|
|
3761
4451
|
<annotation id="G255">
|
|
4452
|
+
<infon key="type">Gene</infon>
|
|
3762
4453
|
<location offset="36841" length="6"/>
|
|
3763
4454
|
<text>ndc1-1</text>
|
|
3764
4455
|
</annotation>
|
|
3765
4456
|
<annotation id="G256">
|
|
4457
|
+
<infon key="type">Gene</infon>
|
|
3766
4458
|
<location offset="36890" length="7"/>
|
|
3767
4459
|
<text>Ndc1-1p</text>
|
|
3768
4460
|
</annotation>
|
|
3769
4461
|
<annotation id="G257">
|
|
4462
|
+
<infon key="score">0.73</infon>
|
|
4463
|
+
<infon key="GeneID">854977</infon>
|
|
4464
|
+
<infon key="type">Gene</infon>
|
|
3770
4465
|
<location offset="37043" length="5"/>
|
|
3771
4466
|
<text>Ndc1p</text>
|
|
3772
4467
|
</annotation>
|
|
3773
4468
|
<annotation id="G258">
|
|
4469
|
+
<infon key="score">0.73</infon>
|
|
4470
|
+
<infon key="GeneID">855159</infon>
|
|
4471
|
+
<infon key="type">Gene</infon>
|
|
3774
4472
|
<location offset="37053" length="7"/>
|
|
3775
4473
|
<text>Pom152p</text>
|
|
3776
4474
|
</annotation>
|
|
3777
4475
|
<annotation id="G259">
|
|
4476
|
+
<infon key="score">0.73</infon>
|
|
4477
|
+
<infon key="GeneID">855159</infon>
|
|
4478
|
+
<infon key="type">Gene</infon>
|
|
3778
4479
|
<location offset="37267" length="7"/>
|
|
3779
4480
|
<text>Pom152p</text>
|
|
3780
4481
|
</annotation>
|
|
3781
4482
|
<annotation id="G260">
|
|
4483
|
+
<infon key="score">0.73</infon>
|
|
4484
|
+
<infon key="GeneID">855159</infon>
|
|
4485
|
+
<infon key="type">Gene</infon>
|
|
3782
4486
|
<location offset="37329" length="7"/>
|
|
3783
4487
|
<text>Pom152p</text>
|
|
3784
4488
|
</annotation>
|
|
3785
4489
|
<annotation id="G261">
|
|
4490
|
+
<infon key="score">0.73</infon>
|
|
4491
|
+
<infon key="GeneID">855159</infon>
|
|
4492
|
+
<infon key="type">Gene</infon>
|
|
3786
4493
|
<location offset="37512" length="7"/>
|
|
3787
4494
|
<text>Pom152p</text>
|
|
3788
4495
|
</annotation>
|
|
3789
4496
|
<annotation id="G262">
|
|
4497
|
+
<infon key="type">Gene</infon>
|
|
3790
4498
|
<location offset="37533" length="7"/>
|
|
3791
4499
|
<text>Ndc1-1p</text>
|
|
3792
4500
|
</annotation>
|
|
@@ -3802,6 +4510,9 @@
|
|
|
3802
4510
|
<text>Ndc1p</text>
|
|
3803
4511
|
</annotation>
|
|
3804
4512
|
<annotation id="G263">
|
|
4513
|
+
<infon key="score">0.73</infon>
|
|
4514
|
+
<infon key="GeneID">854977</infon>
|
|
4515
|
+
<infon key="type">Gene</infon>
|
|
3805
4516
|
<location offset="37971" length="5"/>
|
|
3806
4517
|
<text>Ndc1p</text>
|
|
3807
4518
|
</annotation>
|
|
@@ -4400,386 +5111,493 @@
|
|
|
4400
5111
|
<text>S65T</text>
|
|
4401
5112
|
</annotation>
|
|
4402
5113
|
<annotation id="G264">
|
|
5114
|
+
<infon key="type">Gene</infon>
|
|
4403
5115
|
<location offset="38142" length="325"/>
|
|
4404
5116
|
<text>ProA5-4d/4a MATa/MATalpha NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 ura3-52/ura3-52 his3Delta200/his3Delta200 trp1-1/trp1-1 leu2-3,112/leu2-3,112 lys2-801/lys2-801 ProA5-4d MATaNDC1-ProA-HIS3-URA3 ura3-52 his3Delta200 trp1-1 leu2-3,112 lys2-801 HC26-15a/1a MATa/MATalpha NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 nup49-1</text>
|
|
4405
5117
|
</annotation>
|
|
4406
5118
|
<annotation id="G265">
|
|
5119
|
+
<infon key="type">Gene</infon>
|
|
4407
5120
|
<location offset="38151" length="8"/>
|
|
4408
5121
|
<text>4a MATa</text>
|
|
4409
5122
|
</annotation>
|
|
4410
5123
|
<annotation id="G266">
|
|
5124
|
+
<infon key="type">Gene</infon>
|
|
4411
5125
|
<location offset="38160" length="28"/>
|
|
4412
5126
|
<text>MATalpha NDC1-ProA-HIS3-URA3</text>
|
|
4413
5127
|
</annotation>
|
|
4414
5128
|
<annotation id="G267">
|
|
5129
|
+
<infon key="type">Gene</infon>
|
|
4415
5130
|
<location offset="38189" length="27"/>
|
|
4416
5131
|
<text>NDC1-ProA-HIS3-URA3 ura3-52</text>
|
|
4417
5132
|
</annotation>
|
|
4418
5133
|
<annotation id="G268">
|
|
5134
|
+
<infon key="type">Gene</infon>
|
|
4419
5135
|
<location offset="38217" length="20"/>
|
|
4420
5136
|
<text>ura3-52 his3Delta200</text>
|
|
4421
5137
|
</annotation>
|
|
4422
5138
|
<annotation id="G269">
|
|
5139
|
+
<infon key="type">Gene</infon>
|
|
4423
5140
|
<location offset="38238" length="19"/>
|
|
4424
5141
|
<text>his3Delta200 trp1-1</text>
|
|
4425
5142
|
</annotation>
|
|
4426
5143
|
<annotation id="G270">
|
|
5144
|
+
<infon key="type">Gene</infon>
|
|
4427
5145
|
<location offset="38258" length="21"/>
|
|
4428
5146
|
<text>trp1-1 leu2-3,112</text>
|
|
4429
5147
|
</annotation>
|
|
4430
5148
|
<annotation id="G271">
|
|
5149
|
+
<infon key="type">Gene</infon>
|
|
4431
5150
|
<location offset="38280" length="19"/>
|
|
4432
5151
|
<text>leu2-3,112 lys2-801</text>
|
|
4433
5152
|
</annotation>
|
|
4434
5153
|
<annotation id="G272">
|
|
5154
|
+
<infon key="type">Gene</infon>
|
|
4435
5155
|
<location offset="38300" length="101"/>
|
|
4436
5156
|
<text>lys2-801 ProA5-4d MATaNDC1-ProA-HIS3-URA3 ura3-52 his3Delta200 trp1-1 leu2-3,112 lys2-801 HC26-15a</text>
|
|
4437
5157
|
</annotation>
|
|
4438
5158
|
<annotation id="G273">
|
|
5159
|
+
<infon key="type">Gene</infon>
|
|
4439
5160
|
<location offset="38402" length="8"/>
|
|
4440
5161
|
<text>1a MATa</text>
|
|
4441
5162
|
</annotation>
|
|
4442
5163
|
<annotation id="G274">
|
|
5164
|
+
<infon key="type">Gene</infon>
|
|
4443
5165
|
<location offset="38411" length="28"/>
|
|
4444
5166
|
<text>MATalpha NDC1-ProA-HIS3-URA3</text>
|
|
4445
5167
|
</annotation>
|
|
4446
5168
|
<annotation id="G275">
|
|
5169
|
+
<infon key="type">Gene</infon>
|
|
4447
5170
|
<location offset="38440" length="27"/>
|
|
4448
5171
|
<text>NDC1-ProA-HIS3-URA3 nup49-1</text>
|
|
4449
5172
|
</annotation>
|
|
4450
5173
|
<annotation id="G276">
|
|
5174
|
+
<infon key="type">Gene</infon>
|
|
4451
5175
|
<location offset="38483" length="19"/>
|
|
4452
5176
|
<text>URA3 nup49DeltaGFLG</text>
|
|
4453
5177
|
</annotation>
|
|
4454
5178
|
<annotation id="G277">
|
|
5179
|
+
<infon key="type">Gene</infon>
|
|
4455
5180
|
<location offset="38539" length="110"/>
|
|
4456
5181
|
<text>GFP-S65T-TRP1 ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 HC27-16d/16b MATa/MATalpha nup133Delta</text>
|
|
4457
5182
|
</annotation>
|
|
4458
5183
|
<annotation id="G278">
|
|
5184
|
+
<infon key="type">Gene</infon>
|
|
4459
5185
|
<location offset="38558" length="9"/>
|
|
4460
5186
|
<text>ura3 his3</text>
|
|
4461
5187
|
</annotation>
|
|
4462
5188
|
<annotation id="G279">
|
|
5189
|
+
<infon key="type">Gene</infon>
|
|
4463
5190
|
<location offset="38568" length="11"/>
|
|
4464
5191
|
<text>his3 trp1-1</text>
|
|
4465
5192
|
</annotation>
|
|
4466
5193
|
<annotation id="G280">
|
|
5194
|
+
<infon key="type">Gene</infon>
|
|
4467
5195
|
<location offset="38580" length="17"/>
|
|
4468
5196
|
<text>trp1-1 leu2-3,112</text>
|
|
4469
5197
|
</annotation>
|
|
4470
5198
|
<annotation id="G281">
|
|
5199
|
+
<infon key="type">Gene</infon>
|
|
4471
5200
|
<location offset="38598" length="20"/>
|
|
4472
5201
|
<text>leu2-3,112 HC27-16d</text>
|
|
4473
5202
|
</annotation>
|
|
4474
5203
|
<annotation id="G282">
|
|
5204
|
+
<infon key="type">Gene</infon>
|
|
4475
5205
|
<location offset="38619" length="9"/>
|
|
4476
5206
|
<text>16b MATa</text>
|
|
4477
5207
|
</annotation>
|
|
4478
5208
|
<annotation id="G283">
|
|
5209
|
+
<infon key="type">Gene</infon>
|
|
4479
5210
|
<location offset="38629" length="20"/>
|
|
4480
5211
|
<text>MATalpha nup133Delta</text>
|
|
4481
5212
|
</annotation>
|
|
4482
5213
|
<annotation id="G284">
|
|
5214
|
+
<infon key="type">Gene</infon>
|
|
4483
5215
|
<location offset="38669" length="37"/>
|
|
4484
5216
|
<text>HIS3 NDC1-ProA-HIS3-URA3/NDC1 nup49-1</text>
|
|
4485
5217
|
</annotation>
|
|
4486
5218
|
<annotation id="G285">
|
|
5219
|
+
<infon key="type">Gene</infon>
|
|
4487
5220
|
<location offset="38722" length="23"/>
|
|
4488
5221
|
<text>URA3 nup49DeltaGFLG</text>
|
|
4489
5222
|
</annotation>
|
|
4490
5223
|
<annotation id="G286">
|
|
5224
|
+
<infon key="type">Gene</infon>
|
|
4491
5225
|
<location offset="38747" length="28"/>
|
|
4492
5226
|
<text>GFP-S65T-TRP1/nup49DeltaGFLG</text>
|
|
4493
5227
|
</annotation>
|
|
4494
5228
|
<annotation id="G287">
|
|
5229
|
+
<infon key="type">Gene</infon>
|
|
4495
5230
|
<location offset="38777" length="54"/>
|
|
4496
5231
|
<text>GFP-S65T-TRP1 ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3</text>
|
|
4497
5232
|
</annotation>
|
|
4498
5233
|
<annotation id="G288">
|
|
5234
|
+
<infon key="type">Gene</infon>
|
|
4499
5235
|
<location offset="38860" length="64"/>
|
|
4500
5236
|
<text>MATa/MATalpha NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 spc42Delta</text>
|
|
4501
5237
|
</annotation>
|
|
4502
5238
|
<annotation id="G289">
|
|
5239
|
+
<infon key="type">Gene</infon>
|
|
4503
5240
|
<location offset="38865" length="28"/>
|
|
4504
5241
|
<text>MATalpha NDC1-ProA-HIS3-URA3</text>
|
|
4505
5242
|
</annotation>
|
|
4506
5243
|
<annotation id="G290">
|
|
5244
|
+
<infon key="type">Gene</infon>
|
|
4507
5245
|
<location offset="38894" length="30"/>
|
|
4508
5246
|
<text>NDC1-ProA-HIS3-URA3 spc42Delta</text>
|
|
4509
5247
|
</annotation>
|
|
4510
5248
|
<annotation id="G291">
|
|
5249
|
+
<infon key="type">Gene</infon>
|
|
4511
5250
|
<location offset="38926" length="15"/>
|
|
4512
5251
|
<text>LEU2/spc42Delta</text>
|
|
4513
5252
|
</annotation>
|
|
4514
5253
|
<annotation id="G292">
|
|
5254
|
+
<infon key="type">Gene</infon>
|
|
4515
5255
|
<location offset="38931" length="10"/>
|
|
4516
5256
|
<text>spc42Delta</text>
|
|
4517
5257
|
</annotation>
|
|
4518
5258
|
<annotation id="G293">
|
|
5259
|
+
<infon key="type">Gene</infon>
|
|
4519
5260
|
<location offset="38943" length="13"/>
|
|
4520
5261
|
<text>LEU2 TRP1</text>
|
|
4521
5262
|
</annotation>
|
|
4522
5263
|
<annotation id="G294">
|
|
5264
|
+
<infon key="score">0.73</infon>
|
|
5265
|
+
<infon key="GeneID">853824</infon>
|
|
5266
|
+
<infon key="type">Gene</infon>
|
|
4523
5267
|
<location offset="38958" length="9"/>
|
|
4524
5268
|
<text>SPC42-GFP</text>
|
|
4525
5269
|
</annotation>
|
|
4526
5270
|
<annotation id="G295">
|
|
5271
|
+
<infon key="type">Gene</infon>
|
|
4527
5272
|
<location offset="38978" length="165"/>
|
|
4528
5273
|
<text>SPC42-GFP(3x) ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1 HC23-11d/16a MATa/MATalpha NIC96-ProA-HIS3-URA3/NIC96-ProA-HIS3-URA3 spc42Delta</text>
|
|
4529
5274
|
</annotation>
|
|
4530
5275
|
<annotation id="G296">
|
|
5276
|
+
<infon key="type">Gene</infon>
|
|
4531
5277
|
<location offset="38997" length="9"/>
|
|
4532
5278
|
<text>ura3 his3</text>
|
|
4533
5279
|
</annotation>
|
|
4534
5280
|
<annotation id="G297">
|
|
5281
|
+
<infon key="type">Gene</infon>
|
|
4535
5282
|
<location offset="39007" length="11"/>
|
|
4536
5283
|
<text>his3 trp1-1</text>
|
|
4537
5284
|
</annotation>
|
|
4538
5285
|
<annotation id="G298">
|
|
5286
|
+
<infon key="type">Gene</infon>
|
|
4539
5287
|
<location offset="39019" length="17"/>
|
|
4540
5288
|
<text>trp1-1 leu2-3,112</text>
|
|
4541
5289
|
</annotation>
|
|
4542
5290
|
<annotation id="G299">
|
|
5291
|
+
<infon key="type">Gene</infon>
|
|
4543
5292
|
<location offset="39037" length="17"/>
|
|
4544
5293
|
<text>leu2-3,112 ade2-1</text>
|
|
4545
5294
|
</annotation>
|
|
4546
5295
|
<annotation id="G300">
|
|
5296
|
+
<infon key="type">Gene</infon>
|
|
4547
5297
|
<location offset="39055" length="16"/>
|
|
4548
5298
|
<text>ade2-1 HC23-11d</text>
|
|
4549
5299
|
</annotation>
|
|
4550
5300
|
<annotation id="G301">
|
|
5301
|
+
<infon key="type">Gene</infon>
|
|
4551
5302
|
<location offset="39072" length="9"/>
|
|
4552
5303
|
<text>16a MATa</text>
|
|
4553
5304
|
</annotation>
|
|
4554
5305
|
<annotation id="G302">
|
|
5306
|
+
<infon key="type">Gene</infon>
|
|
4555
5307
|
<location offset="39082" length="29"/>
|
|
4556
5308
|
<text>MATalpha NIC96-ProA-HIS3-URA3</text>
|
|
4557
5309
|
</annotation>
|
|
4558
5310
|
<annotation id="G303">
|
|
5311
|
+
<infon key="type">Gene</infon>
|
|
4559
5312
|
<location offset="39112" length="31"/>
|
|
4560
5313
|
<text>NIC96-ProA-HIS3-URA3 spc42Delta</text>
|
|
4561
5314
|
</annotation>
|
|
4562
5315
|
<annotation id="G304">
|
|
5316
|
+
<infon key="type">Gene</infon>
|
|
4563
5317
|
<location offset="39145" length="15"/>
|
|
4564
5318
|
<text>LEU2/spc42Delta</text>
|
|
4565
5319
|
</annotation>
|
|
4566
5320
|
<annotation id="G305">
|
|
5321
|
+
<infon key="type">Gene</infon>
|
|
4567
5322
|
<location offset="39150" length="10"/>
|
|
4568
5323
|
<text>spc42Delta</text>
|
|
4569
5324
|
</annotation>
|
|
4570
5325
|
<annotation id="G306">
|
|
5326
|
+
<infon key="type">Gene</infon>
|
|
4571
5327
|
<location offset="39162" length="9"/>
|
|
4572
5328
|
<text>LEU2 TRP1</text>
|
|
4573
5329
|
</annotation>
|
|
4574
5330
|
<annotation id="G307">
|
|
5331
|
+
<infon key="type">Gene</infon>
|
|
4575
5332
|
<location offset="39173" length="14"/>
|
|
4576
5333
|
<text>SPC42- GFP</text>
|
|
4577
5334
|
</annotation>
|
|
4578
5335
|
<annotation id="G308">
|
|
5336
|
+
<infon key="type">Gene</infon>
|
|
4579
5337
|
<location offset="39198" length="93"/>
|
|
4580
5338
|
<text>SPC42-GFP(3x) ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1 HC14-10c</text>
|
|
4581
5339
|
</annotation>
|
|
4582
5340
|
<annotation id="G309">
|
|
5341
|
+
<infon key="type">Gene</infon>
|
|
4583
5342
|
<location offset="39307" length="23"/>
|
|
4584
5343
|
<text>MATa/MATalpha ndc1Delta</text>
|
|
4585
5344
|
</annotation>
|
|
4586
5345
|
<annotation id="G310">
|
|
5346
|
+
<infon key="type">Gene</infon>
|
|
4587
5347
|
<location offset="39312" length="18"/>
|
|
4588
5348
|
<text>MATalpha ndc1Delta</text>
|
|
4589
5349
|
</annotation>
|
|
4590
5350
|
<annotation id="G311">
|
|
5351
|
+
<infon key="type">Gene</infon>
|
|
4591
5352
|
<location offset="39349" length="150"/>
|
|
4592
5353
|
<text>HIS3 ura3-52/ura3-52 his3Delta200/his3Delta200 trp1Delta63/TRP1 leu2Delta1/leu2Delta1 LYS2/ lys2-801 ade2Delta426/ade2Delta426 ade3Delta/ade3Delta</text>
|
|
4593
5354
|
</annotation>
|
|
4594
5355
|
<annotation id="G312">
|
|
5356
|
+
<infon key="type">Gene</infon>
|
|
4595
5357
|
<location offset="39362" length="20"/>
|
|
4596
5358
|
<text>ura3-52 his3Delta200</text>
|
|
4597
5359
|
</annotation>
|
|
4598
5360
|
<annotation id="G313">
|
|
5361
|
+
<infon key="type">Gene</infon>
|
|
4599
5362
|
<location offset="39383" length="24"/>
|
|
4600
5363
|
<text>his3Delta200 trp1Delta63</text>
|
|
4601
5364
|
</annotation>
|
|
4602
5365
|
<annotation id="G314">
|
|
5366
|
+
<infon key="type">Gene</infon>
|
|
4603
5367
|
<location offset="39408" length="15"/>
|
|
4604
5368
|
<text>TRP1 leu2Delta1</text>
|
|
4605
5369
|
</annotation>
|
|
4606
5370
|
<annotation id="G315">
|
|
5371
|
+
<infon key="type">Gene</infon>
|
|
4607
5372
|
<location offset="39424" length="15"/>
|
|
4608
5373
|
<text>leu2Delta1 LYS2</text>
|
|
4609
5374
|
</annotation>
|
|
4610
5375
|
<annotation id="G316">
|
|
5376
|
+
<infon key="type">Gene</infon>
|
|
4611
5377
|
<location offset="39445" length="21"/>
|
|
4612
5378
|
<text>lys2-801 ade2Delta426</text>
|
|
4613
5379
|
</annotation>
|
|
4614
5380
|
<annotation id="G317">
|
|
5381
|
+
<infon key="type">Gene</infon>
|
|
4615
5382
|
<location offset="39467" length="22"/>
|
|
4616
5383
|
<text>ade2Delta426 ade3Delta</text>
|
|
4617
5384
|
</annotation>
|
|
4618
5385
|
<annotation id="G318">
|
|
5386
|
+
<infon key="type">Gene</infon>
|
|
4619
5387
|
<location offset="39490" length="9"/>
|
|
4620
5388
|
<text>ade3Delta</text>
|
|
4621
5389
|
</annotation>
|
|
4622
5390
|
<annotation id="G319">
|
|
5391
|
+
<infon key="type">Gene</infon>
|
|
4623
5392
|
<location offset="39501" length="15"/>
|
|
4624
5393
|
<text>pRS315-NDC1-GFP</text>
|
|
4625
5394
|
</annotation>
|
|
4626
5395
|
<annotation id="G320">
|
|
5396
|
+
<infon key="type">Gene</infon>
|
|
4627
5397
|
<location offset="39533" length="511"/>
|
|
4628
5398
|
<text>MATa/MATalpha ura3-52/ura3-52 ade2/ade2 his4-539/HIS4 HC10-42a/42d MATa/MATalpha ndc1-1/ndc1-1 ura3-52/ura3-52 ade2/ade2 his4-539/HIS4 HC13-1c MATalpha NIC96-ProA-HIS3-URA3 ura3-52 his3Delta200 trp1-1 leu2-3,112 lys2-801 Wx340-3c MATalpha ndc1-1 tub2-403 ura3-52 his4-539 lys2-801 Wx340-3d MATalpha tub2-403 ura3-52 his4-539 HC14-10c MATaura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta HC5-31c MATandc1-1 ura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta HC5-31c</text>
|
|
4629
5399
|
</annotation>
|
|
4630
5400
|
<annotation id="G321">
|
|
5401
|
+
<infon key="type">Gene</infon>
|
|
4631
5402
|
<location offset="39538" length="16"/>
|
|
4632
5403
|
<text>MATalpha ura3-52</text>
|
|
4633
5404
|
</annotation>
|
|
4634
5405
|
<annotation id="G322">
|
|
5406
|
+
<infon key="type">Gene</infon>
|
|
4635
5407
|
<location offset="39555" length="12"/>
|
|
4636
5408
|
<text>ura3-52 ade2</text>
|
|
4637
5409
|
</annotation>
|
|
4638
5410
|
<annotation id="G323">
|
|
5411
|
+
<infon key="type">Gene</infon>
|
|
4639
5412
|
<location offset="39568" length="13"/>
|
|
4640
5413
|
<text>ade2 his4-539</text>
|
|
4641
5414
|
</annotation>
|
|
4642
5415
|
<annotation id="G324">
|
|
5416
|
+
<infon key="type">Gene</infon>
|
|
4643
5417
|
<location offset="39582" length="14"/>
|
|
4644
5418
|
<text>HIS4 HC10-42a</text>
|
|
4645
5419
|
</annotation>
|
|
4646
5420
|
<annotation id="G325">
|
|
5421
|
+
<infon key="type">Gene</infon>
|
|
4647
5422
|
<location offset="39597" length="9"/>
|
|
4648
5423
|
<text>42d MATa</text>
|
|
4649
5424
|
</annotation>
|
|
4650
5425
|
<annotation id="G326">
|
|
5426
|
+
<infon key="type">Gene</infon>
|
|
4651
5427
|
<location offset="39607" length="15"/>
|
|
4652
5428
|
<text>MATalpha ndc1-1</text>
|
|
4653
5429
|
</annotation>
|
|
4654
5430
|
<annotation id="G327">
|
|
5431
|
+
<infon key="type">Gene</infon>
|
|
4655
5432
|
<location offset="39623" length="14"/>
|
|
4656
5433
|
<text>ndc1-1 ura3-52</text>
|
|
4657
5434
|
</annotation>
|
|
4658
5435
|
<annotation id="G328">
|
|
5436
|
+
<infon key="type">Gene</infon>
|
|
4659
5437
|
<location offset="39638" length="12"/>
|
|
4660
5438
|
<text>ura3-52 ade2</text>
|
|
4661
5439
|
</annotation>
|
|
4662
5440
|
<annotation id="G329">
|
|
5441
|
+
<infon key="type">Gene</infon>
|
|
4663
5442
|
<location offset="39651" length="13"/>
|
|
4664
5443
|
<text>ade2 his4-539</text>
|
|
4665
5444
|
</annotation>
|
|
4666
5445
|
<annotation id="G330">
|
|
5446
|
+
<infon key="type">Gene</infon>
|
|
4667
5447
|
<location offset="39665" length="379"/>
|
|
4668
5448
|
<text>HIS4 HC13-1c MATalpha NIC96-ProA-HIS3-URA3 ura3-52 his3Delta200 trp1-1 leu2-3,112 lys2-801 Wx340-3c MATalpha ndc1-1 tub2-403 ura3-52 his4-539 lys2-801 Wx340-3d MATalpha tub2-403 ura3-52 his4-539 HC14-10c MATaura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta HC5-31c MATandc1-1 ura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta HC5-31c</text>
|
|
4669
5449
|
</annotation>
|
|
4670
5450
|
<annotation id="G331">
|
|
5451
|
+
<infon key="type">Gene</infon>
|
|
4671
5452
|
<location offset="40052" length="22"/>
|
|
4672
5453
|
<text>MATandc1-1 pom152Delta</text>
|
|
4673
5454
|
</annotation>
|
|
4674
5455
|
<annotation id="G332">
|
|
5456
|
+
<infon key="type">Gene</infon>
|
|
4675
5457
|
<location offset="40076" length="71"/>
|
|
4676
5458
|
<text>HIS3 ura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta</text>
|
|
4677
5459
|
</annotation>
|
|
4678
5460
|
<annotation id="TEAM_277_94">
|
|
5461
|
+
<infon key="type">Gene</infon>
|
|
4679
5462
|
<location offset="38160" length="307"/>
|
|
4680
5463
|
<text>MATalpha NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 ura3-52/ura3-52 his3Delta200/his3Delta200 trp1-1/trp1-1 leu2-3,112/leu2-3,112 lys2-801/lys2-801 ProA5-4d MATaNDC1-ProA-HIS3-URA3 ura3-52 his3Delta200 trp1-1 leu2-3,112 lys2-801 HC26-15a/1a MATa/MATalpha NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 nup49-1</text>
|
|
4681
5464
|
</annotation>
|
|
4682
5465
|
<annotation id="TEAM_277_95">
|
|
5466
|
+
<infon key="type">Gene</infon>
|
|
5467
|
+
<infon key="GeneID">13745</infon>
|
|
4683
5468
|
<location offset="38469" length="4"/>
|
|
4684
5469
|
<text>URA3</text>
|
|
4685
5470
|
</annotation>
|
|
4686
5471
|
<annotation id="TEAM_277_96">
|
|
5472
|
+
<infon key="type">Gene</infon>
|
|
4687
5473
|
<location offset="38539" length="28"/>
|
|
4688
5474
|
<text>GFP-S65T-TRP1 ura3/ura3 his3</text>
|
|
4689
5475
|
</annotation>
|
|
4690
5476
|
<annotation id="TEAM_277_97">
|
|
5477
|
+
<infon key="type">Gene</infon>
|
|
4691
5478
|
<location offset="38568" length="81"/>
|
|
4692
5479
|
<text>his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 HC27-16d/16b MATa/MATalpha nup133Delta</text>
|
|
4693
5480
|
</annotation>
|
|
4694
5481
|
<annotation id="TEAM_277_98">
|
|
5482
|
+
<infon key="type">Gene</infon>
|
|
5483
|
+
<infon key="GeneID">393202</infon>
|
|
4695
5484
|
<location offset="38651" length="4"/>
|
|
4696
5485
|
<text>HIS3</text>
|
|
4697
5486
|
</annotation>
|
|
4698
5487
|
<annotation id="TEAM_277_99">
|
|
5488
|
+
<infon key="type">Gene</infon>
|
|
5489
|
+
<infon key="GeneID">393202</infon>
|
|
4699
5490
|
<location offset="38669" length="4"/>
|
|
4700
5491
|
<text>HIS3</text>
|
|
4701
5492
|
</annotation>
|
|
4702
5493
|
<annotation id="TEAM_277_100">
|
|
5494
|
+
<infon key="type">Gene</infon>
|
|
4703
5495
|
<location offset="38694" length="4"/>
|
|
4704
5496
|
<text>NDC1</text>
|
|
4705
5497
|
</annotation>
|
|
4706
5498
|
<annotation id="TEAM_277_101">
|
|
5499
|
+
<infon key="type">Gene</infon>
|
|
5500
|
+
<infon key="GeneID">13745</infon>
|
|
4707
5501
|
<location offset="38708" length="4"/>
|
|
4708
5502
|
<text>URA3</text>
|
|
4709
5503
|
</annotation>
|
|
4710
5504
|
<annotation id="TEAM_277_102">
|
|
5505
|
+
<infon key="type">Gene</infon>
|
|
4711
5506
|
<location offset="38777" length="28"/>
|
|
4712
5507
|
<text>GFP-S65T-TRP1 ura3/ura3 his3</text>
|
|
4713
5508
|
</annotation>
|
|
4714
5509
|
<annotation id="TEAM_277_103">
|
|
5510
|
+
<infon key="type">Gene</infon>
|
|
4715
5511
|
<location offset="38806" length="87"/>
|
|
4716
5512
|
<text>his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 HC22-2b/1c MATa/MATalpha NDC1-ProA-HIS3-URA3</text>
|
|
4717
5513
|
</annotation>
|
|
4718
5514
|
<annotation id="TEAM_277_104">
|
|
5515
|
+
<infon key="type">Gene</infon>
|
|
5516
|
+
<infon key="GeneID">9980</infon>
|
|
4719
5517
|
<location offset="38926" length="4"/>
|
|
4720
5518
|
<text>LEU2</text>
|
|
4721
5519
|
</annotation>
|
|
4722
5520
|
<annotation id="TEAM_277_105">
|
|
5521
|
+
<infon key="type">Gene</infon>
|
|
5522
|
+
<infon key="GeneID">9980</infon>
|
|
4723
5523
|
<location offset="38943" length="4"/>
|
|
4724
5524
|
<text>LEU2</text>
|
|
4725
5525
|
</annotation>
|
|
4726
5526
|
<annotation id="TEAM_277_106">
|
|
5527
|
+
<infon key="type">Gene</infon>
|
|
4727
5528
|
<location offset="38952" length="4"/>
|
|
4728
5529
|
<text>TRP1</text>
|
|
4729
5530
|
</annotation>
|
|
4730
5531
|
<annotation id="TEAM_277_107">
|
|
5532
|
+
<infon key="type">Gene</infon>
|
|
4731
5533
|
<location offset="38972" length="4"/>
|
|
4732
5534
|
<text>TRP1</text>
|
|
4733
5535
|
</annotation>
|
|
4734
5536
|
<annotation id="TEAM_277_108">
|
|
5537
|
+
<infon key="type">Gene</infon>
|
|
4735
5538
|
<location offset="38978" length="28"/>
|
|
4736
5539
|
<text>SPC42-GFP(3x) ura3/ura3 his3</text>
|
|
4737
5540
|
</annotation>
|
|
4738
5541
|
<annotation id="TEAM_277_109">
|
|
5542
|
+
<infon key="type">Gene</infon>
|
|
4739
5543
|
<location offset="39007" length="104"/>
|
|
4740
5544
|
<text>his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1 HC23-11d/16a MATa/MATalpha NIC96-ProA-HIS3-URA3</text>
|
|
4741
5545
|
</annotation>
|
|
4742
5546
|
<annotation id="TEAM_277_110">
|
|
5547
|
+
<infon key="type">Gene</infon>
|
|
5548
|
+
<infon key="GeneID">9980</infon>
|
|
4743
5549
|
<location offset="39145" length="4"/>
|
|
4744
5550
|
<text>LEU2</text>
|
|
4745
5551
|
</annotation>
|
|
4746
5552
|
<annotation id="TEAM_277_111">
|
|
5553
|
+
<infon key="type">Gene</infon>
|
|
5554
|
+
<infon key="GeneID">9980</infon>
|
|
4747
5555
|
<location offset="39162" length="4"/>
|
|
4748
5556
|
<text>LEU2</text>
|
|
4749
5557
|
</annotation>
|
|
4750
5558
|
<annotation id="TEAM_277_112">
|
|
5559
|
+
<infon key="type">Gene</infon>
|
|
4751
5560
|
<location offset="39167" length="4"/>
|
|
4752
5561
|
<text>TRP1</text>
|
|
4753
5562
|
</annotation>
|
|
4754
5563
|
<annotation id="TEAM_277_113">
|
|
5564
|
+
<infon key="type">Gene</infon>
|
|
4755
5565
|
<location offset="39173" length="23"/>
|
|
4756
5566
|
<text>SPC42- GFP(3x)/TRP1</text>
|
|
4757
5567
|
</annotation>
|
|
4758
5568
|
<annotation id="TEAM_277_114">
|
|
5569
|
+
<infon key="type">Gene</infon>
|
|
4759
5570
|
<location offset="39198" length="28"/>
|
|
4760
5571
|
<text>SPC42-GFP(3x) ura3/ura3 his3</text>
|
|
4761
5572
|
</annotation>
|
|
4762
5573
|
<annotation id="TEAM_277_115">
|
|
5574
|
+
<infon key="type">Gene</infon>
|
|
4763
5575
|
<location offset="39227" length="84"/>
|
|
4764
5576
|
<text>his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1 HC14-10c(1198)/HC29-6b MATa</text>
|
|
4765
5577
|
</annotation>
|
|
4766
5578
|
<annotation id="TEAM_277_116">
|
|
5579
|
+
<infon key="type">Gene</infon>
|
|
5580
|
+
<infon key="GeneID">393202</infon>
|
|
4767
5581
|
<location offset="39349" length="4"/>
|
|
4768
5582
|
<text>HIS3</text>
|
|
4769
5583
|
</annotation>
|
|
4770
5584
|
<annotation id="TEAM_277_117">
|
|
5585
|
+
<infon key="type">Gene</infon>
|
|
4771
5586
|
<location offset="39408" length="173"/>
|
|
4772
5587
|
<text>TRP1 leu2Delta1/leu2Delta1 LYS2/ lys2-801 ade2Delta426/ade2Delta426 ade3Delta/ade3Delta (pRS315-NDC1-GFP) HC10-42b/42c MATa/MATalpha ura3-52/ura3-52 ade2/ade2 his4-539</text>
|
|
4773
5588
|
</annotation>
|
|
4774
5589
|
<annotation id="TEAM_277_118">
|
|
5590
|
+
<infon key="type">Gene</infon>
|
|
4775
5591
|
<location offset="39582" length="82"/>
|
|
4776
5592
|
<text>HIS4 HC10-42a/42d MATa/MATalpha ndc1-1/ndc1-1 ura3-52/ura3-52 ade2/ade2 his4-539</text>
|
|
4777
5593
|
</annotation>
|
|
4778
5594
|
<annotation id="TEAM_277_119">
|
|
5595
|
+
<infon key="type">Gene</infon>
|
|
4779
5596
|
<location offset="39665" length="409"/>
|
|
4780
5597
|
<text>HIS4 HC13-1c MATalpha NIC96-ProA-HIS3-URA3 ura3-52 his3Delta200 trp1-1 leu2-3,112 lys2-801 Wx340-3c MATalpha ndc1-1 tub2-403 ura3-52 his4-539 lys2-801 Wx340-3d MATalpha tub2-403 ura3-52 his4-539 HC14-10c MATaura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta HC5-31c MATandc1-1 ura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta HC5-31c(1166) MATandc1-1 pom152Delta</text>
|
|
4781
5598
|
</annotation>
|
|
4782
5599
|
<annotation id="TEAM_277_120">
|
|
5600
|
+
<infon key="type">Gene</infon>
|
|
4783
5601
|
<location offset="40076" length="71"/>
|
|
4784
5602
|
<text>HIS3 ura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta</text>
|
|
4785
5603
|
</annotation>
|
|
@@ -4842,42 +5660,64 @@
|
|
|
4842
5660
|
<text>GFP</text>
|
|
4843
5661
|
</annotation>
|
|
4844
5662
|
<annotation id="G333">
|
|
5663
|
+
<infon key="type">Gene</infon>
|
|
4845
5664
|
<location offset="40065" length="10"/>
|
|
4846
5665
|
<text>Ndc1p-ProA</text>
|
|
4847
5666
|
</annotation>
|
|
4848
5667
|
<annotation id="G334">
|
|
5668
|
+
<infon key="type">Gene</infon>
|
|
4849
5669
|
<location offset="40454" length="10"/>
|
|
4850
5670
|
<text>Ndc1p-ProA</text>
|
|
4851
5671
|
</annotation>
|
|
4852
5672
|
<annotation id="G335">
|
|
5673
|
+
<infon key="score">0.73</infon>
|
|
5674
|
+
<infon key="GeneID">852703</infon>
|
|
5675
|
+
<infon key="type">Gene</infon>
|
|
4853
5676
|
<location offset="40469" length="10"/>
|
|
4854
5677
|
<text>Nup49p-GFP</text>
|
|
4855
5678
|
</annotation>
|
|
4856
5679
|
<annotation id="G336">
|
|
5680
|
+
<infon key="score">0.73</infon>
|
|
5681
|
+
<infon key="GeneID">852703</infon>
|
|
5682
|
+
<infon key="type">Gene</infon>
|
|
4857
5683
|
<location offset="40596" length="10"/>
|
|
4858
5684
|
<text>Nup49p-GFP</text>
|
|
4859
5685
|
</annotation>
|
|
4860
5686
|
<annotation id="G337">
|
|
5687
|
+
<infon key="score">0.73</infon>
|
|
5688
|
+
<infon key="GeneID">7011691</infon>
|
|
5689
|
+
<infon key="type">Gene</infon>
|
|
4861
5690
|
<location offset="40710" length="3"/>
|
|
4862
5691
|
<text>GFP</text>
|
|
4863
5692
|
</annotation>
|
|
4864
5693
|
<annotation id="G338">
|
|
5694
|
+
<infon key="type">Gene</infon>
|
|
4865
5695
|
<location offset="40795" length="10"/>
|
|
4866
5696
|
<text>Ndc1p-ProA</text>
|
|
4867
5697
|
</annotation>
|
|
4868
5698
|
<annotation id="G339">
|
|
5699
|
+
<infon key="score">0.73</infon>
|
|
5700
|
+
<infon key="GeneID">852703</infon>
|
|
5701
|
+
<infon key="type">Gene</infon>
|
|
4869
5702
|
<location offset="40823" length="10"/>
|
|
4870
5703
|
<text>Nup49p-GFP</text>
|
|
4871
5704
|
</annotation>
|
|
4872
5705
|
<annotation id="G340">
|
|
5706
|
+
<infon key="type">Gene</infon>
|
|
4873
5707
|
<location offset="40871" length="18"/>
|
|
4874
5708
|
<text>nup133 null allele</text>
|
|
4875
5709
|
</annotation>
|
|
4876
5710
|
<annotation id="G341">
|
|
5711
|
+
<infon key="score">0.73</infon>
|
|
5712
|
+
<infon key="GeneID">852703</infon>
|
|
5713
|
+
<infon key="type">Gene</infon>
|
|
4877
5714
|
<location offset="41007" length="10"/>
|
|
4878
5715
|
<text>Nup49p-GFP</text>
|
|
4879
5716
|
</annotation>
|
|
4880
5717
|
<annotation id="G342">
|
|
5718
|
+
<infon key="score">0.73</infon>
|
|
5719
|
+
<infon key="GeneID">7011691</infon>
|
|
5720
|
+
<infon key="type">Gene</infon>
|
|
4881
5721
|
<location offset="41093" length="3"/>
|
|
4882
5722
|
<text>GFP</text>
|
|
4883
5723
|
</annotation>
|
|
@@ -4965,50 +5805,75 @@
|
|
|
4965
5805
|
<text>Pom152p is a NPC component (Wozniak et al., 1994), and Spc110p is a SPB component (Rout and Kilmartin, 1990; Kilmartin et al., 1993)</text>
|
|
4966
5806
|
</annotation>
|
|
4967
5807
|
<annotation id="G343">
|
|
5808
|
+
<infon key="type">Gene</infon>
|
|
4968
5809
|
<location offset="41161" length="10"/>
|
|
4969
5810
|
<text>Ndc1p-ProA</text>
|
|
4970
5811
|
</annotation>
|
|
4971
5812
|
<annotation id="G344">
|
|
5813
|
+
<infon key="type">Gene</infon>
|
|
4972
5814
|
<location offset="41297" length="9"/>
|
|
4973
5815
|
<text>Kilmartin</text>
|
|
4974
5816
|
</annotation>
|
|
4975
5817
|
<annotation id="G345">
|
|
5818
|
+
<infon key="type">Gene</infon>
|
|
4976
5819
|
<location offset="41324" length="9"/>
|
|
4977
5820
|
<text>Kilmartin</text>
|
|
4978
5821
|
</annotation>
|
|
4979
5822
|
<annotation id="G346">
|
|
5823
|
+
<infon key="score">0.73</infon>
|
|
5824
|
+
<infon key="GeneID">855159</infon>
|
|
5825
|
+
<infon key="type">Gene</infon>
|
|
4980
5826
|
<location offset="41546" length="7"/>
|
|
4981
5827
|
<text>Pom152p</text>
|
|
4982
5828
|
</annotation>
|
|
4983
5829
|
<annotation id="G347">
|
|
5830
|
+
<infon key="score">0.73</infon>
|
|
5831
|
+
<infon key="GeneID">851957</infon>
|
|
5832
|
+
<infon key="type">Gene</infon>
|
|
4984
5833
|
<location offset="41570" length="7"/>
|
|
4985
5834
|
<text>Spc110p</text>
|
|
4986
5835
|
</annotation>
|
|
4987
5836
|
<annotation id="G348">
|
|
5837
|
+
<infon key="score">0.73</infon>
|
|
5838
|
+
<infon key="GeneID">855159</infon>
|
|
5839
|
+
<infon key="type">Gene</infon>
|
|
4988
5840
|
<location offset="41579" length="7"/>
|
|
4989
5841
|
<text>Pom152p</text>
|
|
4990
5842
|
</annotation>
|
|
4991
5843
|
<annotation id="G349">
|
|
5844
|
+
<infon key="score">0.73</infon>
|
|
5845
|
+
<infon key="GeneID">851957</infon>
|
|
5846
|
+
<infon key="type">Gene</infon>
|
|
4992
5847
|
<location offset="41635" length="7"/>
|
|
4993
5848
|
<text>Spc110p</text>
|
|
4994
5849
|
</annotation>
|
|
4995
5850
|
<annotation id="G350">
|
|
5851
|
+
<infon key="type">Gene</infon>
|
|
4996
5852
|
<location offset="41672" length="9"/>
|
|
4997
5853
|
<text>Kilmartin</text>
|
|
4998
5854
|
</annotation>
|
|
4999
5855
|
<annotation id="G351">
|
|
5856
|
+
<infon key="type">Gene</infon>
|
|
5000
5857
|
<location offset="42156" length="10"/>
|
|
5001
5858
|
<text>Ndc1p-ProA</text>
|
|
5002
5859
|
</annotation>
|
|
5003
5860
|
<annotation id="G352">
|
|
5861
|
+
<infon key="score">0.73</infon>
|
|
5862
|
+
<infon key="GeneID">855159</infon>
|
|
5863
|
+
<infon key="type">Gene</infon>
|
|
5004
5864
|
<location offset="42363" length="7"/>
|
|
5005
5865
|
<text>Pom152p</text>
|
|
5006
5866
|
</annotation>
|
|
5007
5867
|
<annotation id="G353">
|
|
5868
|
+
<infon key="score">0.73</infon>
|
|
5869
|
+
<infon key="GeneID">853016</infon>
|
|
5870
|
+
<infon key="type">Gene</infon>
|
|
5008
5871
|
<location offset="42376" length="6"/>
|
|
5009
5872
|
<text>Nup57p</text>
|
|
5010
5873
|
</annotation>
|
|
5011
5874
|
<annotation id="TEAM_277_121">
|
|
5875
|
+
<infon key="type">Gene</infon>
|
|
5876
|
+
<infon key="GeneID">4415</infon>
|
|
5012
5877
|
<location offset="42376" length="6"/>
|
|
5013
5878
|
<text>Nup57p</text>
|
|
5014
5879
|
</annotation>
|
|
@@ -5060,42 +5925,66 @@
|
|
|
5060
5925
|
<text>nup133</text>
|
|
5061
5926
|
</annotation>
|
|
5062
5927
|
<annotation id="G354">
|
|
5928
|
+
<infon key="type">Gene</infon>
|
|
5063
5929
|
<location offset="42384" length="10"/>
|
|
5064
5930
|
<text>Ndc1p-ProA</text>
|
|
5065
5931
|
</annotation>
|
|
5066
5932
|
<annotation id="G355">
|
|
5933
|
+
<infon key="type">Gene</infon>
|
|
5067
5934
|
<location offset="42514" length="10"/>
|
|
5068
5935
|
<text>Ndc1p-ProA</text>
|
|
5069
5936
|
</annotation>
|
|
5070
5937
|
<annotation id="G356">
|
|
5938
|
+
<infon key="score">0.73</infon>
|
|
5939
|
+
<infon key="GeneID">853824</infon>
|
|
5940
|
+
<infon key="type">Gene</infon>
|
|
5071
5941
|
<location offset="42530" length="10"/>
|
|
5072
5942
|
<text>Spc42p-GFP</text>
|
|
5073
5943
|
</annotation>
|
|
5074
5944
|
<annotation id="G357">
|
|
5945
|
+
<infon key="score">0.73</infon>
|
|
5946
|
+
<infon key="GeneID">853824</infon>
|
|
5947
|
+
<infon key="type">Gene</infon>
|
|
5075
5948
|
<location offset="42622" length="10"/>
|
|
5076
5949
|
<text>Spc42p-GFP</text>
|
|
5077
5950
|
</annotation>
|
|
5078
5951
|
<annotation id="G358">
|
|
5952
|
+
<infon key="type">Gene</infon>
|
|
5079
5953
|
<location offset="42886" length="11"/>
|
|
5080
5954
|
<text>Nic96p-ProA</text>
|
|
5081
5955
|
</annotation>
|
|
5082
5956
|
<annotation id="G359">
|
|
5957
|
+
<infon key="score">0.73</infon>
|
|
5958
|
+
<infon key="GeneID">853824</infon>
|
|
5959
|
+
<infon key="type">Gene</infon>
|
|
5083
5960
|
<location offset="42902" length="10"/>
|
|
5084
5961
|
<text>Spc42p-GFP</text>
|
|
5085
5962
|
</annotation>
|
|
5086
5963
|
<annotation id="G360">
|
|
5964
|
+
<infon key="score">0.73</infon>
|
|
5965
|
+
<infon key="GeneID">852703</infon>
|
|
5966
|
+
<infon key="type">Gene</infon>
|
|
5087
5967
|
<location offset="43172" length="10"/>
|
|
5088
5968
|
<text>Nup49p-GFP</text>
|
|
5089
5969
|
</annotation>
|
|
5090
5970
|
<annotation id="G361">
|
|
5971
|
+
<infon key="score">0.70</infon>
|
|
5972
|
+
<infon key="GeneID">406852</infon>
|
|
5973
|
+
<infon key="type">Gene</infon>
|
|
5091
5974
|
<location offset="43188" length="6"/>
|
|
5092
5975
|
<text>nup133</text>
|
|
5093
5976
|
</annotation>
|
|
5094
5977
|
<annotation id="G362">
|
|
5978
|
+
<infon key="score">0.73</infon>
|
|
5979
|
+
<infon key="GeneID">852703</infon>
|
|
5980
|
+
<infon key="type">Gene</infon>
|
|
5095
5981
|
<location offset="43302" length="10"/>
|
|
5096
5982
|
<text>Nup49p-GFP</text>
|
|
5097
5983
|
</annotation>
|
|
5098
5984
|
<annotation id="G363">
|
|
5985
|
+
<infon key="score">0.73</infon>
|
|
5986
|
+
<infon key="GeneID">852703</infon>
|
|
5987
|
+
<infon key="type">Gene</infon>
|
|
5099
5988
|
<location offset="43571" length="10"/>
|
|
5100
5989
|
<text>Nup49p-GFP</text>
|
|
5101
5990
|
</annotation>
|
|
@@ -5123,34 +6012,49 @@
|
|
|
5123
6012
|
<text>GFP</text>
|
|
5124
6013
|
</annotation>
|
|
5125
6014
|
<annotation id="G364">
|
|
6015
|
+
<infon key="score">0.73</infon>
|
|
6016
|
+
<infon key="GeneID">854977</infon>
|
|
6017
|
+
<infon key="type">Gene</infon>
|
|
5126
6018
|
<location offset="43592" length="9"/>
|
|
5127
6019
|
<text>Ndc1p-GFP</text>
|
|
5128
6020
|
</annotation>
|
|
5129
6021
|
<annotation id="G365">
|
|
6022
|
+
<infon key="type">Gene</infon>
|
|
5130
6023
|
<location offset="43754" length="16"/>
|
|
5131
6024
|
<text>ndc1 null allele</text>
|
|
5132
6025
|
</annotation>
|
|
5133
6026
|
<annotation id="G366">
|
|
6027
|
+
<infon key="score">0.70</infon>
|
|
6028
|
+
<infon key="GeneID">335236</infon>
|
|
6029
|
+
<infon key="type">Gene</infon>
|
|
5134
6030
|
<location offset="43830" length="8"/>
|
|
5135
6031
|
<text>NDC1-GFP</text>
|
|
5136
6032
|
</annotation>
|
|
5137
6033
|
<annotation id="G367">
|
|
6034
|
+
<infon key="score">0.73</infon>
|
|
6035
|
+
<infon key="GeneID">854977</infon>
|
|
6036
|
+
<infon key="type">Gene</infon>
|
|
5138
6037
|
<location offset="44143" length="9"/>
|
|
5139
6038
|
<text>Ndc1p-GFP</text>
|
|
5140
6039
|
</annotation>
|
|
5141
6040
|
<annotation id="TEAM_277_122">
|
|
6041
|
+
<infon key="type">Gene</infon>
|
|
5142
6042
|
<location offset="43667" length="9"/>
|
|
5143
6043
|
<text>Ndc1p-GFP</text>
|
|
5144
6044
|
</annotation>
|
|
5145
6045
|
<annotation id="TEAM_277_123">
|
|
6046
|
+
<infon key="type">Gene</infon>
|
|
5146
6047
|
<location offset="43877" length="9"/>
|
|
5147
6048
|
<text>Ndc1p-GFP</text>
|
|
5148
6049
|
</annotation>
|
|
5149
6050
|
<annotation id="TEAM_277_124">
|
|
6051
|
+
<infon key="type">Gene</infon>
|
|
5150
6052
|
<location offset="44143" length="9"/>
|
|
5151
6053
|
<text>Ndc1p-GFP</text>
|
|
5152
6054
|
</annotation>
|
|
5153
6055
|
<annotation id="5">
|
|
6056
|
+
<infon key="type">ExperimentalMethod</infon>
|
|
6057
|
+
<infon key="PSIMI">0416</infon>
|
|
5154
6058
|
<location offset="43877" length="226"/>
|
|
5155
6059
|
<text>Ndc1p-GFP localization was observed in living cells using deconvolution fluorescence microscopy (A). Additionally, this localization was examined using immunoelectron microscopy of whole cells (see Materials and Methods).</text>
|
|
5156
6060
|
</annotation>
|
|
@@ -5198,26 +6102,34 @@
|
|
|
5198
6102
|
<text>FITC</text>
|
|
5199
6103
|
</annotation>
|
|
5200
6104
|
<annotation id="G368">
|
|
6105
|
+
<infon key="type">Gene</infon>
|
|
5201
6106
|
<location offset="44326" length="6"/>
|
|
5202
6107
|
<text>ndc1-1</text>
|
|
5203
6108
|
</annotation>
|
|
5204
6109
|
<annotation id="G369">
|
|
6110
|
+
<infon key="type">Gene</infon>
|
|
5205
6111
|
<location offset="44495" length="12"/>
|
|
5206
6112
|
<text>HC10-42b/42c</text>
|
|
5207
6113
|
</annotation>
|
|
5208
6114
|
<annotation id="G370">
|
|
6115
|
+
<infon key="score">0.70</infon>
|
|
6116
|
+
<infon key="GeneID">20194</infon>
|
|
6117
|
+
<infon key="type">Gene</infon>
|
|
5209
6118
|
<location offset="44504" length="3"/>
|
|
5210
6119
|
<text>42c</text>
|
|
5211
6120
|
</annotation>
|
|
5212
6121
|
<annotation id="G371">
|
|
6122
|
+
<infon key="type">Gene</infon>
|
|
5213
6123
|
<location offset="44571" length="10"/>
|
|
5214
6124
|
<text>NIC96-ProA</text>
|
|
5215
6125
|
</annotation>
|
|
5216
6126
|
<annotation id="G372">
|
|
6127
|
+
<infon key="type">Gene</infon>
|
|
5217
6128
|
<location offset="44647" length="15"/>
|
|
5218
6129
|
<text>pGAL-NIC96-ProA</text>
|
|
5219
6130
|
</annotation>
|
|
5220
6131
|
<annotation id="G373">
|
|
6132
|
+
<infon key="type">Gene</infon>
|
|
5221
6133
|
<location offset="44749" length="14"/>
|
|
5222
6134
|
<text>ndc1-1 culture</text>
|
|
5223
6135
|
</annotation>
|
|
@@ -5334,94 +6246,125 @@
|
|
|
5334
6246
|
<text>pom152</text>
|
|
5335
6247
|
</annotation>
|
|
5336
6248
|
<annotation id="G374">
|
|
6249
|
+
<infon key="score">0.70</infon>
|
|
6250
|
+
<infon key="GeneID">855159</infon>
|
|
6251
|
+
<infon key="type">Gene</infon>
|
|
5337
6252
|
<location offset="45121" length="6"/>
|
|
5338
6253
|
<text>POM152</text>
|
|
5339
6254
|
</annotation>
|
|
5340
6255
|
<annotation id="G375">
|
|
6256
|
+
<infon key="type">Gene</infon>
|
|
5341
6257
|
<location offset="45143" length="10"/>
|
|
5342
6258
|
<text>ndc1-1 SPB</text>
|
|
5343
6259
|
</annotation>
|
|
5344
6260
|
<annotation id="G376">
|
|
6261
|
+
<infon key="type">Gene</infon>
|
|
5345
6262
|
<location offset="45202" length="6"/>
|
|
5346
6263
|
<text>ndc1-1</text>
|
|
5347
6264
|
</annotation>
|
|
5348
6265
|
<annotation id="G377">
|
|
6266
|
+
<infon key="type">Gene</infon>
|
|
5349
6267
|
<location offset="45212" length="6"/>
|
|
5350
6268
|
<text>ndc1-1</text>
|
|
5351
6269
|
</annotation>
|
|
5352
6270
|
<annotation id="G378">
|
|
6271
|
+
<infon key="score">0.70</infon>
|
|
6272
|
+
<infon key="GeneID">855159</infon>
|
|
6273
|
+
<infon key="type">Gene</infon>
|
|
5353
6274
|
<location offset="45220" length="6"/>
|
|
5354
6275
|
<text>pom152</text>
|
|
5355
6276
|
</annotation>
|
|
5356
6277
|
<annotation id="G379">
|
|
6278
|
+
<infon key="type">Gene</infon>
|
|
5357
6279
|
<location offset="45317" length="10"/>
|
|
5358
6280
|
<text>pURA3-NDC1</text>
|
|
5359
6281
|
</annotation>
|
|
5360
6282
|
<annotation id="G380">
|
|
6283
|
+
<infon key="type">Gene</infon>
|
|
5361
6284
|
<location offset="45381" length="10"/>
|
|
5362
6285
|
<text>pURA3-NDC1</text>
|
|
5363
6286
|
</annotation>
|
|
5364
6287
|
<annotation id="G381">
|
|
6288
|
+
<infon key="type">Gene</infon>
|
|
5365
6289
|
<location offset="45429" length="5"/>
|
|
5366
6290
|
<text>pLEU2</text>
|
|
5367
6291
|
</annotation>
|
|
5368
6292
|
<annotation id="G382">
|
|
6293
|
+
<infon key="type">Gene</infon>
|
|
5369
6294
|
<location offset="45439" length="7"/>
|
|
5370
6295
|
<text>pPM1-HA</text>
|
|
5371
6296
|
</annotation>
|
|
5372
6297
|
<annotation id="G383">
|
|
6298
|
+
<infon key="type">Gene</infon>
|
|
5373
6299
|
<location offset="45593" length="6"/>
|
|
5374
6300
|
<text>ndc1-1</text>
|
|
5375
6301
|
</annotation>
|
|
5376
6302
|
<annotation id="G384">
|
|
6303
|
+
<infon key="type">Gene</infon>
|
|
5377
6304
|
<location offset="45615" length="10"/>
|
|
5378
6305
|
<text>pURA3-NDC1</text>
|
|
5379
6306
|
</annotation>
|
|
5380
6307
|
<annotation id="G385">
|
|
6308
|
+
<infon key="type">Gene</infon>
|
|
5381
6309
|
<location offset="45656" length="6"/>
|
|
5382
6310
|
<text>ndc1-1</text>
|
|
5383
6311
|
</annotation>
|
|
5384
6312
|
<annotation id="G386">
|
|
6313
|
+
<infon key="score">0.70</infon>
|
|
6314
|
+
<infon key="GeneID">855159</infon>
|
|
6315
|
+
<infon key="type">Gene</infon>
|
|
5385
6316
|
<location offset="45664" length="6"/>
|
|
5386
6317
|
<text>pom152</text>
|
|
5387
6318
|
</annotation>
|
|
5388
6319
|
<annotation id="G387">
|
|
6320
|
+
<infon key="type">Gene</infon>
|
|
5389
6321
|
<location offset="45860" length="6"/>
|
|
5390
6322
|
<text>ndc1-1</text>
|
|
5391
6323
|
</annotation>
|
|
5392
6324
|
<annotation id="G388">
|
|
6325
|
+
<infon key="type">Gene</infon>
|
|
5393
6326
|
<location offset="45933" length="6"/>
|
|
5394
6327
|
<text>ndc1-1</text>
|
|
5395
6328
|
</annotation>
|
|
5396
6329
|
<annotation id="G389">
|
|
6330
|
+
<infon key="type">Gene</infon>
|
|
5397
6331
|
<location offset="45946" length="6"/>
|
|
5398
6332
|
<text>ndc1-1</text>
|
|
5399
6333
|
</annotation>
|
|
5400
6334
|
<annotation id="G390">
|
|
6335
|
+
<infon key="type">Gene</infon>
|
|
5401
6336
|
<location offset="45981" length="6"/>
|
|
5402
6337
|
<text>ndc1-1</text>
|
|
5403
6338
|
</annotation>
|
|
5404
6339
|
<annotation id="G391">
|
|
6340
|
+
<infon key="type">Gene</infon>
|
|
5405
6341
|
<location offset="45989" length="11"/>
|
|
5406
6342
|
<text>pom152Delta</text>
|
|
5407
6343
|
</annotation>
|
|
5408
6344
|
<annotation id="G392">
|
|
6345
|
+
<infon key="type">Gene</infon>
|
|
5409
6346
|
<location offset="46385" length="6"/>
|
|
5410
6347
|
<text>ndc1-1</text>
|
|
5411
6348
|
</annotation>
|
|
5412
6349
|
<annotation id="TEAM_277_125">
|
|
6350
|
+
<infon key="type">Gene</infon>
|
|
5413
6351
|
<location offset="45121" length="6"/>
|
|
5414
6352
|
<text>POM152</text>
|
|
5415
6353
|
</annotation>
|
|
5416
6354
|
<annotation id="TEAM_277_126">
|
|
6355
|
+
<infon key="type">Gene</infon>
|
|
6356
|
+
<infon key="GeneID">12205</infon>
|
|
5417
6357
|
<location offset="45421" length="6"/>
|
|
5418
6358
|
<text>pRS315</text>
|
|
5419
6359
|
</annotation>
|
|
5420
6360
|
<annotation id="TEAM_277_127">
|
|
6361
|
+
<infon key="type">Gene</infon>
|
|
5421
6362
|
<location offset="45781" length="6"/>
|
|
5422
6363
|
<text>POM152</text>
|
|
5423
6364
|
</annotation>
|
|
5424
6365
|
<annotation id="TEAM_277_128">
|
|
6366
|
+
<infon key="type">Gene</infon>
|
|
6367
|
+
<infon key="GeneID">200184</infon>
|
|
5425
6368
|
<location offset="45789" length="12"/>
|
|
5426
6369
|
<text>pLEU2-POM152</text>
|
|
5427
6370
|
</annotation>
|
|
@@ -5492,6 +6435,9 @@
|
|
|
5492
6435
|
<offset>46575</offset>
|
|
5493
6436
|
<text>ProA</text>
|
|
5494
6437
|
<annotation id="G393">
|
|
6438
|
+
<infon key="score">0.70</infon>
|
|
6439
|
+
<infon key="GeneID">7502638</infon>
|
|
6440
|
+
<infon key="type">Gene</infon>
|
|
5495
6441
|
<location offset="46575" length="4"/>
|
|
5496
6442
|
<text>ProA</text>
|
|
5497
6443
|
</annotation>
|
|
@@ -5598,6 +6544,8 @@
|
|
|
5598
6544
|
<text>Pom152p</text>
|
|
5599
6545
|
</annotation>
|
|
5600
6546
|
<annotation id="TEAM_277_129">
|
|
6547
|
+
<infon key="type">Gene</infon>
|
|
6548
|
+
<infon key="GeneID">4415</infon>
|
|
5601
6549
|
<location offset="46751" length="7"/>
|
|
5602
6550
|
<text>Nup157p</text>
|
|
5603
6551
|
</annotation>
|
|
@@ -5736,6 +6684,8 @@
|
|
|
5736
6684
|
<offset>47346</offset>
|
|
5737
6685
|
<text>Identification and characterization of a nuclear pore complex protein</text>
|
|
5738
6686
|
<annotation id="TEAM_277_130">
|
|
6687
|
+
<infon key="type">Gene</infon>
|
|
6688
|
+
<infon key="GeneID">156510</infon>
|
|
5739
6689
|
<location offset="47387" length="28"/>
|
|
5740
6690
|
<text>nuclear pore complex protein</text>
|
|
5741
6691
|
</annotation>
|
|
@@ -5904,6 +6854,8 @@
|
|
|
5904
6854
|
<text>NIC96</text>
|
|
5905
6855
|
</annotation>
|
|
5906
6856
|
<annotation id="TEAM_277_131">
|
|
6857
|
+
<infon key="type">Gene</infon>
|
|
6858
|
+
<infon key="GeneID">795388</infon>
|
|
5907
6859
|
<location offset="48307" length="4"/>
|
|
5908
6860
|
<text>NSP1</text>
|
|
5909
6861
|
</annotation>
|
|
@@ -5947,6 +6899,8 @@
|
|
|
5947
6899
|
<text>Nup57p</text>
|
|
5948
6900
|
</annotation>
|
|
5949
6901
|
<annotation id="TEAM_277_132">
|
|
6902
|
+
<infon key="type">Gene</infon>
|
|
6903
|
+
<infon key="GeneID">461059</infon>
|
|
5950
6904
|
<location offset="48485" length="5"/>
|
|
5951
6905
|
<text>Nsp1p</text>
|
|
5952
6906
|
</annotation>
|
|
@@ -5987,6 +6941,8 @@
|
|
|
5987
6941
|
<text>Nic96p</text>
|
|
5988
6942
|
</annotation>
|
|
5989
6943
|
<annotation id="TEAM_277_133">
|
|
6944
|
+
<infon key="type">Gene</infon>
|
|
6945
|
+
<infon key="GeneID">4415</infon>
|
|
5990
6946
|
<location offset="48526" length="5"/>
|
|
5991
6947
|
<text>Nup93</text>
|
|
5992
6948
|
</annotation>
|
|
@@ -6020,6 +6976,8 @@
|
|
|
6020
6976
|
<text>RAT2</text>
|
|
6021
6977
|
</annotation>
|
|
6022
6978
|
<annotation id="TEAM_277_134">
|
|
6979
|
+
<infon key="type">Gene</infon>
|
|
6980
|
+
<infon key="GeneID">10114</infon>
|
|
6023
6981
|
<location offset="48799" length="4"/>
|
|
6024
6982
|
<text>RAT2</text>
|
|
6025
6983
|
</annotation>
|
|
@@ -6065,6 +7023,8 @@
|
|
|
6065
7023
|
<offset>48912</offset>
|
|
6066
7024
|
<text>Diverse effects of beta-tubulin mutations on microtubule formation and function</text>
|
|
6067
7025
|
<annotation id="TEAM_277_135">
|
|
7026
|
+
<infon key="type">Gene</infon>
|
|
7027
|
+
<infon key="GeneID">130530</infon>
|
|
6068
7028
|
<location offset="48931" length="12"/>
|
|
6069
7029
|
<text>beta-tubulin</text>
|
|
6070
7030
|
</annotation>
|
|
@@ -6303,14 +7263,20 @@
|
|
|
6303
7263
|
<text>Nup57p</text>
|
|
6304
7264
|
</annotation>
|
|
6305
7265
|
<annotation id="TEAM_277_136">
|
|
7266
|
+
<infon key="type">Gene</infon>
|
|
7267
|
+
<infon key="GeneID">461059</infon>
|
|
6306
7268
|
<location offset="49808" length="5"/>
|
|
6307
7269
|
<text>Nsp1p</text>
|
|
6308
7270
|
</annotation>
|
|
6309
7271
|
<annotation id="TEAM_277_137">
|
|
7272
|
+
<infon key="type">Gene</infon>
|
|
7273
|
+
<infon key="GeneID">4415</infon>
|
|
6310
7274
|
<location offset="49815" length="6"/>
|
|
6311
7275
|
<text>Nup49p</text>
|
|
6312
7276
|
</annotation>
|
|
6313
7277
|
<annotation id="TEAM_277_138">
|
|
7278
|
+
<infon key="type">Gene</infon>
|
|
7279
|
+
<infon key="GeneID">4415</infon>
|
|
6314
7280
|
<location offset="49827" length="6"/>
|
|
6315
7281
|
<text>Nup57p</text>
|
|
6316
7282
|
</annotation>
|
|
@@ -6343,6 +7309,8 @@
|
|
|
6343
7309
|
<text>yeast</text>
|
|
6344
7310
|
</annotation>
|
|
6345
7311
|
<annotation id="TEAM_277_139">
|
|
7312
|
+
<infon key="type">Gene</infon>
|
|
7313
|
+
<infon key="GeneID">11988</infon>
|
|
6346
7314
|
<location offset="49870" length="3"/>
|
|
6347
7315
|
<text>TC4</text>
|
|
6348
7316
|
</annotation>
|
|
@@ -6478,6 +7446,7 @@
|
|
|
6478
7446
|
<text>yeast</text>
|
|
6479
7447
|
</annotation>
|
|
6480
7448
|
<annotation id="TEAM_277_140">
|
|
7449
|
+
<infon key="type">Gene</infon>
|
|
6481
7450
|
<location offset="50450" length="21"/>
|
|
6482
7451
|
<text>ribosomal protein L29</text>
|
|
6483
7452
|
</annotation>
|
|
@@ -6592,6 +7561,7 @@
|
|
|
6592
7561
|
<text>yeast</text>
|
|
6593
7562
|
</annotation>
|
|
6594
7563
|
<annotation id="TEAM_277_141">
|
|
7564
|
+
<infon key="type">Gene</infon>
|
|
6595
7565
|
<location offset="50855" length="4"/>
|
|
6596
7566
|
<text>NDC1</text>
|
|
6597
7567
|
</annotation>
|
|
@@ -6734,6 +7704,8 @@
|
|
|
6734
7704
|
<text>Nup188p</text>
|
|
6735
7705
|
</annotation>
|
|
6736
7706
|
<annotation id="TEAM_277_142">
|
|
7707
|
+
<infon key="type">Gene</infon>
|
|
7708
|
+
<infon key="GeneID">4415</infon>
|
|
6737
7709
|
<location offset="51607" length="7"/>
|
|
6738
7710
|
<text>Nup188p</text>
|
|
6739
7711
|
</annotation>
|