simple_bioc 0.0.16 → 0.0.17

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Files changed (16) hide show
  1. checksums.yaml +4 -4
  2. data/lib/simple_bioc/annotation.rb +1 -1
  3. data/lib/simple_bioc/bioc_reader.rb +6 -2
  4. data/lib/simple_bioc/bioc_writer.rb +11 -10
  5. data/lib/simple_bioc/document.rb +5 -5
  6. data/lib/simple_bioc/location_adjuster.rb +3 -1
  7. data/lib/simple_bioc/node.rb +2 -2
  8. data/lib/simple_bioc/node_base.rb +1 -1
  9. data/lib/simple_bioc/passage.rb +5 -5
  10. data/lib/simple_bioc/relation.rb +1 -1
  11. data/lib/simple_bioc/sentence.rb +3 -3
  12. data/lib/simple_bioc/version.rb +1 -1
  13. data/xml/merge/output.xml +974 -974
  14. data/xml/merge/output_10330397.xml +317 -317
  15. data/xml/merge/output_10366597.xml +1 -1
  16. metadata +2 -2
data/xml/merge/output_10330397.xml CHANGED
@@ -96,57 +96,57 @@
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  </annotation>
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  <annotation id="Team286_A14">
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  <infon key="type">Gene</infon>
99
- <location offset="727" length="4"/>
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+ <location offset="728" length="4"/>
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  <text>LST1</text>
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  </annotation>
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  <annotation id="Team286_A15">
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  <infon key="type">Gene</infon>
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- <location offset="778" length="29"/>
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+ <location offset="779" length="29"/>
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  <text>plasma membrane proton-ATPase</text>
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  </annotation>
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  <annotation id="Team286_A16">
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  <infon key="type">Gene</infon>
109
- <location offset="810" length="5"/>
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+ <location offset="811" length="5"/>
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  <text>Pma1p</text>
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  </annotation>
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  <annotation id="Team286_A17">
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  <infon key="type">Gene</infon>
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- <location offset="979" length="5"/>
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+ <location offset="980" length="5"/>
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  <text>Pma1p</text>
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  </annotation>
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  <annotation id="Team286_A18">
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  <infon key="type">Gene</infon>
119
- <location offset="1012" length="17"/>
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+ <location offset="1013" length="17"/>
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  <text>lst1Delta mutants</text>
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  </annotation>
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  <annotation id="Team286_A19">
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  <infon key="type">Gene</infon>
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- <location offset="1110" length="15"/>
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+ <location offset="1112" length="15"/>
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  <text>Pma1p transport</text>
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  </annotation>
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  <annotation id="Team286_A20">
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  <infon key="type">Gene</infon>
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- <location offset="1252" length="18"/>
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+ <location offset="1254" length="18"/>
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  <text>COPII coat subunit</text>
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  </annotation>
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  <annotation id="Team286_A21">
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  <infon key="type">Gene</infon>
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- <location offset="1277" length="5"/>
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+ <location offset="1279" length="5"/>
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  <text>Lst1p</text>
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  </annotation>
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  <annotation id="Team286_A22">
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  <infon key="type">Gene</infon>
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- <location offset="1295" length="6"/>
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+ <location offset="1297" length="6"/>
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  <text>Sec24p</text>
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  </annotation>
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  <annotation id="Team286_A23">
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  <infon key="type">Gene</infon>
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- <location offset="1343" length="5"/>
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+ <location offset="1345" length="5"/>
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  <text>Pma1p</text>
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  </annotation>
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  <annotation id="TEAM_336_PPI0">
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  <infon key="type">PPImention</infon>
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- <location offset="608" length="94"/>
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+ <location offset="608" length="94" original_offset="0"/>
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  <text>Like Sec24p, Lst1p is a peripheral ER membrane protein that binds to the COPII subunit Sec23p.</text>
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  </annotation>
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  </passage>
@@ -326,12 +326,12 @@
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  </annotation>
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  <annotation id="TEAM_336_PPI1">
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  <infon key="type">PPImention</infon>
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- <location offset="3406" length="348"/>
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+ <location offset="3406" length="348" original_offset="0"/>
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  <text>Membrane-associated Sar1p, in turn, recruits the soluble Sec23p/Sec24p and Sec13p/Sec31p complexes (Matsuoka et al., 1998). Sec16p resides on the ER membrane and binds to both the Sec23p/Sec24p and Sec13p/Sec31p complexes, likely organizing their assembly onto the membrane (Espenshade et al., 1995; Gimeno et al., 1996; Shaywitz et al., 1997).</text>
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  </annotation>
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  <annotation id="TEAM_336_PPI2">
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  <infon key="type">PPImention</infon>
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- <location offset="3963" length="291"/>
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+ <location offset="3963" length="291" original_offset="0"/>
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  <text>Association of a membrane-bound complex of Sar1p and Sec23p/Sec24p with integral membrane proteins indicates that cargo proteins may laterally partition into the vesicle membrane by virtue of their affinity for the Sec23p/Sec24p protein complex (Aridor et al., 1998; Kuehn et al., 1998).</text>
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  </annotation>
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  </passage>
@@ -356,7 +356,7 @@
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  </annotation>
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  <annotation id="Team286_A60">
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  <infon key="type">Gene</infon>
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- <location offset="5068" length="10"/>
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+ <location offset="5069" length="10"/>
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  <text>COPII coat</text>
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  </annotation>
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  </passage>
@@ -401,62 +401,62 @@
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  </annotation>
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  <annotation id="Team286_A68">
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  <infon key="type">Gene</infon>
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- <location offset="5564" length="4"/>
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+ <location offset="5565" length="4"/>
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  <text>LST1</text>
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  </annotation>
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  <annotation id="Team286_A69">
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  <infon key="type">Gene</infon>
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- <location offset="5718" length="4"/>
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+ <location offset="5719" length="4"/>
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  <text>LST1</text>
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  </annotation>
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  <annotation id="Team286_A70">
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  <infon key="type">Gene</infon>
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- <location offset="5789" length="4"/>
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+ <location offset="5790" length="4"/>
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  <text>LST1</text>
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  </annotation>
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  <annotation id="Team286_A71">
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  <infon key="type">Gene</infon>
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- <location offset="5822" length="13"/>
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+ <location offset="5823" length="13"/>
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  <text>COPII subunit</text>
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  </annotation>
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  <annotation id="Team286_A72">
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  <infon key="type">Gene</infon>
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- <location offset="5837" length="6"/>
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+ <location offset="5838" length="6"/>
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  <text>Sec24p</text>
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  </annotation>
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  <annotation id="Team286_A73">
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  <infon key="type">Gene</infon>
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- <location offset="5854" length="5"/>
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+ <location offset="5855" length="5"/>
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  <text>Lst1p</text>
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  </annotation>
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  <annotation id="Team286_A74">
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  <infon key="type">Gene</infon>
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- <location offset="5943" length="6"/>
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+ <location offset="5944" length="6"/>
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  <text>Sec23p</text>
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  </annotation>
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  <annotation id="Team286_A75">
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  <infon key="type">Gene</infon>
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- <location offset="5955" length="9"/>
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+ <location offset="5956" length="9"/>
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  <text>LST1 gene</text>
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  </annotation>
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  <annotation id="Team286_A76">
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  <infon key="type">Gene</infon>
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- <location offset="6024" length="17"/>
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+ <location offset="6025" length="17"/>
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  <text>lst1Delta mutants</text>
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  </annotation>
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  <annotation id="Team286_A77">
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  <infon key="type">Gene</infon>
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- <location offset="6056" length="4"/>
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+ <location offset="6057" length="4"/>
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  <text>LST1</text>
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  </annotation>
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  <annotation id="Team286_A78">
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  <infon key="type">Gene</infon>
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- <location offset="6101" length="5"/>
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+ <location offset="6102" length="5"/>
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  <text>Pma1p</text>
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  </annotation>
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  <annotation id="Team286_A79">
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  <infon key="type">Gene</infon>
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- <location offset="6246" length="5"/>
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+ <location offset="6247" length="5"/>
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  <text>Pma1p</text>
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  </annotation>
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  </passage>
@@ -711,42 +711,42 @@
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  </annotation>
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  <annotation id="Team286_A126">
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  <infon key="type">Gene</infon>
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- <location offset="8575" length="11"/>
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+ <location offset="8576" length="11"/>
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  <text>HA epitopes</text>
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  </annotation>
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  <annotation id="Team286_A127">
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  <infon key="type">Gene</infon>
719
- <location offset="8604" length="7"/>
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+ <location offset="8605" length="7"/>
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  <text>pKR17HA</text>
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  </annotation>
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  <annotation id="Team286_A128">
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  <infon key="type">Gene</infon>
724
- <location offset="8613" length="7"/>
724
+ <location offset="8615" length="7"/>
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  <text>pKR17HA</text>
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  </annotation>
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  <annotation id="Team286_A129">
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  <infon key="type">Gene</infon>
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- <location offset="8642" length="6"/>
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+ <location offset="8644" length="6"/>
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  <text>CKY536</text>
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  </annotation>
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732
  <annotation id="Team286_A130">
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  <infon key="type">Gene</infon>
734
- <location offset="8657" length="6"/>
734
+ <location offset="8659" length="6"/>
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735
  <text>CKY535</text>
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  </annotation>
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737
  <annotation id="Team286_A131">
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  <infon key="type">Gene</infon>
739
- <location offset="8665" length="14"/>
739
+ <location offset="8667" length="14"/>
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  <text>MATa lst1Delta</text>
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  </annotation>
742
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  <annotation id="Team286_A132">
743
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  <infon key="type">Gene</infon>
744
- <location offset="8683" length="24"/>
744
+ <location offset="8685" length="24"/>
745
745
  <text>LEU2 leu2-3, 112 ura3-52</text>
746
746
  </annotation>
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  <annotation id="Team286_A133">
748
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  <infon key="type">Gene</infon>
749
- <location offset="8709" length="7"/>
749
+ <location offset="8711" length="7"/>
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  <text>pKR17HA</text>
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  </annotation>
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  </passage>
@@ -886,22 +886,22 @@
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  </annotation>
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  <annotation id="Team286_A158">
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  <infon key="type">Gene</infon>
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- <location offset="9815" length="7"/>
889
+ <location offset="9816" length="7"/>
890
890
  <text>sec13-1</text>
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891
  </annotation>
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892
  <annotation id="Team286_A159">
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  <infon key="type">Gene</infon>
894
- <location offset="9954" length="4"/>
894
+ <location offset="9955" length="4"/>
895
895
  <text>pKR1</text>
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896
  </annotation>
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  <annotation id="Team286_A160">
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  <infon key="type">Gene</infon>
899
- <location offset="9994" length="6"/>
899
+ <location offset="9995" length="6"/>
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  <text>pRS316</text>
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901
  </annotation>
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  <annotation id="Team286_A161">
903
903
  <infon key="type">Gene</infon>
904
- <location offset="10018" length="7"/>
904
+ <location offset="10019" length="7"/>
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  <text>sec13-1</text>
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906
  </annotation>
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907
  </passage>
@@ -1186,82 +1186,82 @@
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  </annotation>
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  <annotation id="Team286_A213">
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  <infon key="type">Gene</infon>
1189
- <location offset="12374" length="4"/>
1189
+ <location offset="12375" length="4"/>
1190
1190
  <text>LST1</text>
1191
1191
  </annotation>
1192
1192
  <annotation id="Team286_A214">
1193
1193
  <infon key="type">Gene</infon>
1194
- <location offset="12437" length="8"/>
1194
+ <location offset="12438" length="8"/>
1195
1195
  <text>Eco47III</text>
1196
1196
  </annotation>
1197
1197
  <annotation id="Team286_A215">
1198
1198
  <infon key="type">Gene</infon>
1199
- <location offset="12446" length="4"/>
1199
+ <location offset="12447" length="4"/>
1200
1200
  <text>MscI</text>
1201
1201
  </annotation>
1202
1202
  <annotation id="Team286_A216">
1203
1203
  <infon key="type">Gene</infon>
1204
- <location offset="12465" length="5"/>
1204
+ <location offset="12466" length="5"/>
1205
1205
  <text>pKR28</text>
1206
1206
  </annotation>
1207
1207
  <annotation id="Team286_A217">
1208
1208
  <infon key="type">Gene</infon>
1209
- <location offset="12483" length="10"/>
1209
+ <location offset="12484" length="10"/>
1210
1210
  <text>pKR28Delta</text>
1211
1211
  </annotation>
1212
1212
  <annotation id="Team286_A218">
1213
1213
  <infon key="type">Gene</infon>
1214
- <location offset="12510" length="14"/>
1214
+ <location offset="12511" length="14"/>
1215
1215
  <text>LEU2 construct</text>
1216
1216
  </annotation>
1217
1217
  <annotation id="Team286_A219">
1218
1218
  <infon key="type">Gene</infon>
1219
- <location offset="12541" length="10"/>
1219
+ <location offset="12542" length="10"/>
1220
1220
  <text>pKR28Delta</text>
1221
1221
  </annotation>
1222
1222
  <annotation id="Team286_A220">
1223
1223
  <infon key="type">Gene</infon>
1224
- <location offset="12570" length="4"/>
1224
+ <location offset="12571" length="4"/>
1225
1225
  <text>XhoI</text>
1226
1226
  </annotation>
1227
1227
  <annotation id="Team286_A221">
1228
1228
  <infon key="type">Gene</infon>
1229
- <location offset="12627" length="6"/>
1229
+ <location offset="12628" length="6"/>
1230
1230
  <text>CKY348</text>
1231
1231
  </annotation>
1232
1232
  <annotation id="Team286_A222">
1233
1233
  <infon key="type">Gene</infon>
1234
- <location offset="12635" length="28"/>
1234
+ <location offset="12636" length="28"/>
1235
1235
  <text>MATa/alpha leu2-3,112/leu2-3</text>
1236
1236
  </annotation>
1237
1237
  <annotation id="Team286_A223">
1238
1238
  <infon key="type">Gene</infon>
1239
- <location offset="12664" length="3"/>
1239
+ <location offset="12665" length="3"/>
1240
1240
  <text>112</text>
1241
1241
  </annotation>
1242
1242
  <annotation id="Team286_A224">
1243
1243
  <infon key="type">Gene</infon>
1244
- <location offset="12668" length="7"/>
1244
+ <location offset="12669" length="7"/>
1245
1245
  <text>ura3-52</text>
1246
1246
  </annotation>
1247
1247
  <annotation id="Team286_A225">
1248
1248
  <infon key="type">Gene</infon>
1249
- <location offset="12676" length="7"/>
1249
+ <location offset="12677" length="7"/>
1250
1250
  <text>ura3-52</text>
1251
1251
  </annotation>
1252
1252
  <annotation id="Team286_A226">
1253
1253
  <infon key="type">Gene</infon>
1254
- <location offset="12794" length="9"/>
1254
+ <location offset="12795" length="9"/>
1255
1255
  <text>lst1Delta</text>
1256
1256
  </annotation>
1257
1257
  <annotation id="Team286_A227">
1258
1258
  <infon key="type">Gene</infon>
1259
- <location offset="12805" length="4"/>
1259
+ <location offset="12806" length="4"/>
1260
1260
  <text>LEU2</text>
1261
1261
  </annotation>
1262
1262
  <annotation id="Team286_A228">
1263
1263
  <infon key="type">Gene</infon>
1264
- <location offset="12951" length="6"/>
1264
+ <location offset="12952" length="6"/>
1265
1265
  <text>CKY542</text>
1266
1266
  </annotation>
1267
1267
  </passage>
@@ -1666,7 +1666,7 @@
1666
1666
  </annotation>
1667
1667
  <annotation id="TEAM_336_PPI3">
1668
1668
  <infon key="type">PPImention</infon>
1669
- <location offset="21609" length="26"/>
1669
+ <location offset="21609" length="26" original_offset="0"/>
1670
1670
  <text>Binding of Lst1p to Sec23p</text>
1671
1671
  </annotation>
1672
1672
  </passage>
@@ -1766,32 +1766,32 @@
1766
1766
  </annotation>
1767
1767
  <annotation id="Team286_A310">
1768
1768
  <infon key="type">Gene</infon>
1769
- <location offset="22102" length="6"/>
1769
+ <location offset="22103" length="6"/>
1770
1770
  <text>CKY473</text>
1771
1771
  </annotation>
1772
1772
  <annotation id="Team286_A311">
1773
1773
  <infon key="type">Gene</infon>
1774
- <location offset="22127" length="6"/>
1774
+ <location offset="22128" length="6"/>
1775
1775
  <text>pRH254</text>
1776
1776
  </annotation>
1777
1777
  <annotation id="Team286_A312">
1778
1778
  <infon key="type">Gene</infon>
1779
- <location offset="22135" length="3"/>
1779
+ <location offset="22136" length="3"/>
1780
1780
  <text>GST</text>
1781
1781
  </annotation>
1782
1782
  <annotation id="Team286_A313">
1783
1783
  <infon key="type">Gene</infon>
1784
- <location offset="22139" length="5"/>
1784
+ <location offset="22140" length="5"/>
1785
1785
  <text>Lst1p</text>
1786
1786
  </annotation>
1787
1787
  <annotation id="Team286_A314">
1788
1788
  <infon key="type">Gene</infon>
1789
- <location offset="22178" length="6"/>
1789
+ <location offset="22179" length="6"/>
1790
1790
  <text>pPE123</text>
1791
1791
  </annotation>
1792
1792
  <annotation id="Team286_A315">
1793
1793
  <infon key="type">Gene</infon>
1794
- <location offset="22186" length="6"/>
1794
+ <location offset="22187" length="6"/>
1795
1795
  <text>Sec23p</text>
1796
1796
  </annotation>
1797
1797
  </passage>
@@ -2186,182 +2186,182 @@
2186
2186
  </annotation>
2187
2187
  <annotation id="Team286_A384">
2188
2188
  <infon key="type">Gene</infon>
2189
- <location offset="27979" length="6"/>
2189
+ <location offset="27980" length="6"/>
2190
2190
  <text>CKY426</text>
2191
2191
  </annotation>
2192
2192
  <annotation id="Team286_A385">
2193
2193
  <infon key="type">Gene</infon>
2194
- <location offset="28201" length="5"/>
2194
+ <location offset="28202" length="5"/>
2195
2195
  <text>SEC13</text>
2196
2196
  </annotation>
2197
2197
  <annotation id="Team286_A386">
2198
2198
  <infon key="type">Gene</infon>
2199
- <location offset="28211" length="4"/>
2199
+ <location offset="28212" length="4"/>
2200
2200
  <text>LST1</text>
2201
2201
  </annotation>
2202
2202
  <annotation id="Team286_A387">
2203
2203
  <infon key="type">Gene</infon>
2204
- <location offset="28279" length="5"/>
2204
+ <location offset="28280" length="5"/>
2205
2205
  <text>SEC13</text>
2206
2206
  </annotation>
2207
2207
  <annotation id="Team286_A388">
2208
2208
  <infon key="type">Gene</infon>
2209
- <location offset="28379" length="7"/>
2209
+ <location offset="28380" length="7"/>
2210
2210
  <text>sec13-1</text>
2211
2211
  </annotation>
2212
2212
  <annotation id="Team286_A389">
2213
2213
  <infon key="type">Gene</infon>
2214
- <location offset="28516" length="6"/>
2214
+ <location offset="28517" length="6"/>
2215
2215
  <text>p21-31</text>
2216
2216
  </annotation>
2217
2217
  <annotation id="Team286_A390">
2218
2218
  <infon key="type">Gene</infon>
2219
- <location offset="28527" length="5"/>
2219
+ <location offset="28528" length="5"/>
2220
2220
  <text>p77-2</text>
2221
2221
  </annotation>
2222
2222
  <annotation id="Team286_A391">
2223
2223
  <infon key="type">Gene</infon>
2224
- <location offset="28610" length="5"/>
2224
+ <location offset="28611" length="5"/>
2225
2225
  <text>p77-2</text>
2226
2226
  </annotation>
2227
2227
  <annotation id="Team286_A392">
2228
2228
  <infon key="type">Gene</infon>
2229
- <location offset="28693" length="4"/>
2229
+ <location offset="28694" length="4"/>
2230
2230
  <text>XhoI</text>
2231
2231
  </annotation>
2232
2232
  <annotation id="Team286_A393">
2233
2233
  <infon key="type">Gene</infon>
2234
- <location offset="28753" length="5"/>
2234
+ <location offset="28754" length="5"/>
2235
2235
  <text>pKR20</text>
2236
2236
  </annotation>
2237
2237
  <annotation id="Team286_A394">
2238
2238
  <infon key="type">Gene</infon>
2239
- <location offset="28790" length="5"/>
2239
+ <location offset="28791" length="5"/>
2240
2240
  <text>pKR20</text>
2241
2241
  </annotation>
2242
2242
  <annotation id="Team286_A395">
2243
2243
  <infon key="type">Gene</infon>
2244
- <location offset="28829" length="4"/>
2244
+ <location offset="28830" length="4"/>
2245
2245
  <text>HpaI</text>
2246
2246
  </annotation>
2247
2247
  <annotation id="Team286_A396">
2248
2248
  <infon key="type">Gene</infon>
2249
- <location offset="28856" length="6"/>
2249
+ <location offset="28857" length="6"/>
2250
2250
  <text>CUY564</text>
2251
2251
  </annotation>
2252
2252
  <annotation id="Team286_A397">
2253
2253
  <infon key="type">Gene</infon>
2254
- <location offset="28864" length="29"/>
2254
+ <location offset="28865" length="29"/>
2255
2255
  <text>MATalpha ade2 ade3 leu2 ura3</text>
2256
2256
  </annotation>
2257
2257
  <annotation id="Team286_A398">
2258
2258
  <infon key="type">Gene</infon>
2259
- <location offset="28936" length="21"/>
2259
+ <location offset="28937" length="21"/>
2260
2260
  <text>lst1-1 mutant CKY426</text>
2261
2261
  </annotation>
2262
2262
  <annotation id="Team286_A399">
2263
2263
  <infon key="type">Gene</infon>
2264
- <location offset="28959" length="4"/>
2264
+ <location offset="28960" length="4"/>
2265
2265
  <text>MATa</text>
2266
2266
  </annotation>
2267
2267
  <annotation id="Team286_A400">
2268
2268
  <infon key="type">Gene</infon>
2269
- <location offset="28964" length="19"/>
2269
+ <location offset="28965" length="19"/>
2270
2270
  <text>lst1-1 sec13-1 ade2</text>
2271
2271
  </annotation>
2272
2272
  <annotation id="Team286_A401">
2273
2273
  <infon key="type">Gene</infon>
2274
- <location offset="28984" length="14"/>
2274
+ <location offset="28985" length="14"/>
2275
2275
  <text>ade3 leu2 ura3</text>
2276
2276
  </annotation>
2277
2277
  <annotation id="Team286_A402">
2278
2278
  <infon key="type">Gene</infon>
2279
- <location offset="29001" length="4"/>
2279
+ <location offset="29002" length="4"/>
2280
2280
  <text>pKR4</text>
2281
2281
  </annotation>
2282
2282
  <annotation id="Team286_A403">
2283
2283
  <infon key="type">Gene</infon>
2284
- <location offset="29059" length="5"/>
2284
+ <location offset="29060" length="5"/>
2285
2285
  <text>pKR20</text>
2286
2286
  </annotation>
2287
2287
  <annotation id="Team286_A404">
2288
2288
  <infon key="type">Gene</infon>
2289
- <location offset="29106" length="10"/>
2289
+ <location offset="29107" length="10"/>
2290
2290
  <text>LST1 locus</text>
2291
2291
  </annotation>
2292
2292
  <annotation id="Team286_A405">
2293
2293
  <infon key="type">Gene</infon>
2294
- <location offset="29175" length="4"/>
2294
+ <location offset="29176" length="4"/>
2295
2295
  <text>Ura+</text>
2296
2296
  </annotation>
2297
2297
  <annotation id="Team286_A406">
2298
2298
  <infon key="type">Gene</infon>
2299
- <location offset="29219" length="3"/>
2299
+ <location offset="29220" length="3"/>
2300
2300
  <text>Ura</text>
2301
2301
  </annotation>
2302
2302
  <annotation id="Team286_A407">
2303
2303
  <infon key="type">Gene</infon>
2304
- <location offset="29231" length="5"/>
2304
+ <location offset="29233" length="5"/>
2305
2305
  <text>p77-2</text>
2306
2306
  </annotation>
2307
2307
  <annotation id="Team286_A408">
2308
2308
  <infon key="type">Gene</infon>
2309
- <location offset="29249" length="9"/>
2309
+ <location offset="29251" length="9"/>
2310
2310
  <text>LST1 gene</text>
2311
2311
  </annotation>
2312
2312
  <annotation id="Team286_A409">
2313
2313
  <infon key="type">Gene</infon>
2314
- <location offset="29299" length="6"/>
2314
+ <location offset="29301" length="6"/>
2315
2315
  <text>p21-31</text>
2316
2316
  </annotation>
2317
2317
  <annotation id="Team286_A410">
2318
2318
  <infon key="type">Gene</infon>
2319
- <location offset="29378" length="5"/>
2319
+ <location offset="29380" length="5"/>
2320
2320
  <text>EcoRI</text>
2321
2321
  </annotation>
2322
2322
  <annotation id="Team286_A411">
2323
2323
  <infon key="type">Gene</infon>
2324
- <location offset="29384" length="7"/>
2324
+ <location offset="29386" length="7"/>
2325
2325
  <text>HindIII</text>
2326
2326
  </annotation>
2327
2327
  <annotation id="Team286_A412">
2328
2328
  <infon key="type">Gene</infon>
2329
- <location offset="29407" length="6"/>
2329
+ <location offset="29409" length="6"/>
2330
2330
  <text>pRS306</text>
2331
2331
  </annotation>
2332
2332
  <annotation id="Team286_A413">
2333
2333
  <infon key="type">Gene</infon>
2334
- <location offset="29425" length="4"/>
2334
+ <location offset="29427" length="4"/>
2335
2335
  <text>pKR7</text>
2336
2336
  </annotation>
2337
2337
  <annotation id="Team286_A414">
2338
2338
  <infon key="type">Gene</infon>
2339
- <location offset="29431" length="4"/>
2339
+ <location offset="29433" length="4"/>
2340
2340
  <text>pKR7</text>
2341
2341
  </annotation>
2342
2342
  <annotation id="Team286_A415">
2343
2343
  <infon key="type">Gene</infon>
2344
- <location offset="29502" length="4"/>
2344
+ <location offset="29504" length="4"/>
2345
2345
  <text>MscI</text>
2346
2346
  </annotation>
2347
2347
  <annotation id="Team286_A416">
2348
2348
  <infon key="type">Gene</infon>
2349
- <location offset="29543" length="6"/>
2349
+ <location offset="29545" length="6"/>
2350
2350
  <text>lst1-1</text>
2351
2351
  </annotation>
2352
2352
  <annotation id="Team286_A417">
2353
2353
  <infon key="type">Gene</infon>
2354
- <location offset="29580" length="4"/>
2354
+ <location offset="29582" length="4"/>
2355
2355
  <text>pKR7</text>
2356
2356
  </annotation>
2357
2357
  <annotation id="Team286_A418">
2358
2358
  <infon key="type">Gene</infon>
2359
- <location offset="29603" length="4"/>
2359
+ <location offset="29605" length="4"/>
2360
2360
  <text>LST1</text>
2361
2361
  </annotation>
2362
2362
  <annotation id="Team286_A419">
2363
2363
  <infon key="type">Gene</infon>
2364
- <location offset="29631" length="4"/>
2364
+ <location offset="29633" length="4"/>
2365
2365
  <text>pKR7</text>
2366
2366
  </annotation>
2367
2367
  </passage>
@@ -2381,32 +2381,32 @@
2381
2381
  </annotation>
2382
2382
  <annotation id="Team286_A422">
2383
2383
  <infon key="type">Gene</infon>
2384
- <location offset="30102" length="4"/>
2384
+ <location offset="30103" length="4"/>
2385
2385
  <text>LST1</text>
2386
2386
  </annotation>
2387
2387
  <annotation id="Team286_A423">
2388
2388
  <infon key="type">Gene</infon>
2389
- <location offset="30140" length="5"/>
2389
+ <location offset="30141" length="5"/>
2390
2390
  <text>SEC24</text>
2391
2391
  </annotation>
2392
2392
  <annotation id="Team286_A424">
2393
2393
  <infon key="type">Gene</infon>
2394
- <location offset="30147" length="7"/>
2394
+ <location offset="30148" length="7"/>
2395
2395
  <text>YIL109C</text>
2396
2396
  </annotation>
2397
2397
  <annotation id="Team286_A425">
2398
2398
  <infon key="type">Gene</infon>
2399
- <location offset="30268" length="5"/>
2399
+ <location offset="30269" length="5"/>
2400
2400
  <text>Lst1p</text>
2401
2401
  </annotation>
2402
2402
  <annotation id="Team286_A426">
2403
2403
  <infon key="type">Gene</infon>
2404
- <location offset="30314" length="6"/>
2404
+ <location offset="30315" length="6"/>
2405
2405
  <text>Sec24p</text>
2406
2406
  </annotation>
2407
2407
  <annotation id="Team286_A427">
2408
2408
  <infon key="type">Gene</infon>
2409
- <location offset="30342" length="18"/>
2409
+ <location offset="30343" length="18"/>
2410
2410
  <text>COPII vesicle coat</text>
2411
2411
  </annotation>
2412
2412
  </passage>
@@ -2766,87 +2766,87 @@
2766
2766
  </annotation>
2767
2767
  <annotation id="Team286_A494">
2768
2768
  <infon key="type">Gene</infon>
2769
- <location offset="34578" length="18"/>
2769
+ <location offset="34579" length="18"/>
2770
2770
  <text>pGAL1-LST1 plasmid</text>
2771
2771
  </annotation>
2772
2772
  <annotation id="Team286_A495">
2773
2773
  <infon key="type">Gene</infon>
2774
- <location offset="34598" length="5"/>
2774
+ <location offset="34599" length="5"/>
2775
2775
  <text>pKR35</text>
2776
2776
  </annotation>
2777
2777
  <annotation id="Team286_A496">
2778
2778
  <infon key="type">Gene</infon>
2779
- <location offset="34614" length="10"/>
2779
+ <location offset="34615" length="10"/>
2780
2780
  <text>SEC24 gene</text>
2781
2781
  </annotation>
2782
2782
  <annotation id="Team286_A497">
2783
2783
  <infon key="type">Gene</infon>
2784
- <location offset="34643" length="5"/>
2784
+ <location offset="34644" length="5"/>
2785
2785
  <text>pKR41</text>
2786
2786
  </annotation>
2787
2787
  <annotation id="Team286_A498">
2788
2788
  <infon key="type">Gene</infon>
2789
- <location offset="34777" length="5"/>
2789
+ <location offset="34778" length="5"/>
2790
2790
  <text>SEC24</text>
2791
2791
  </annotation>
2792
2792
  <annotation id="Team286_A499">
2793
2793
  <infon key="type">Gene</infon>
2794
- <location offset="34856" length="5"/>
2794
+ <location offset="34857" length="5"/>
2795
2795
  <text>SEC12</text>
2796
2796
  </annotation>
2797
2797
  <annotation id="Team286_A500">
2798
2798
  <infon key="type">Gene</infon>
2799
- <location offset="34863" length="5"/>
2799
+ <location offset="34864" length="5"/>
2800
2800
  <text>SEC13</text>
2801
2801
  </annotation>
2802
2802
  <annotation id="Team286_A501">
2803
2803
  <infon key="type">Gene</infon>
2804
- <location offset="34870" length="5"/>
2804
+ <location offset="34871" length="5"/>
2805
2805
  <text>SEC31</text>
2806
2806
  </annotation>
2807
2807
  <annotation id="Team286_A502">
2808
2808
  <infon key="type">Gene</infon>
2809
- <location offset="34880" length="5"/>
2809
+ <location offset="34881" length="5"/>
2810
2810
  <text>SEC23</text>
2811
2811
  </annotation>
2812
2812
  <annotation id="Team286_A503">
2813
2813
  <infon key="type">Gene</infon>
2814
- <location offset="34939" length="5"/>
2814
+ <location offset="34940" length="5"/>
2815
2815
  <text>SEC23</text>
2816
2816
  </annotation>
2817
2817
  <annotation id="Team286_A504">
2818
2818
  <infon key="type">Gene</infon>
2819
- <location offset="35076" length="5"/>
2819
+ <location offset="35077" length="5"/>
2820
2820
  <text>SEC23</text>
2821
2821
  </annotation>
2822
2822
  <annotation id="Team286_A505">
2823
2823
  <infon key="type">Gene</infon>
2824
- <location offset="35134" length="5"/>
2824
+ <location offset="35135" length="5"/>
2825
2825
  <text>SEC23</text>
2826
2826
  </annotation>
2827
2827
  <annotation id="Team286_A506">
2828
2828
  <infon key="type">Gene</infon>
2829
- <location offset="35263" length="5"/>
2829
+ <location offset="35264" length="5"/>
2830
2830
  <text>Lst1p</text>
2831
2831
  </annotation>
2832
2832
  <annotation id="Team286_A507">
2833
2833
  <infon key="type">Gene</infon>
2834
- <location offset="35330" length="5"/>
2834
+ <location offset="35331" length="5"/>
2835
2835
  <text>Lst1p</text>
2836
2836
  </annotation>
2837
2837
  <annotation id="Team286_A508">
2838
2838
  <infon key="type">Gene</infon>
2839
- <location offset="35340" length="6"/>
2839
+ <location offset="35341" length="6"/>
2840
2840
  <text>Sec24p</text>
2841
2841
  </annotation>
2842
2842
  <annotation id="Team286_A509">
2843
2843
  <infon key="type">Gene</infon>
2844
- <location offset="35391" length="22"/>
2844
+ <location offset="35392" length="22"/>
2845
2845
  <text>vesicle coat complexes</text>
2846
2846
  </annotation>
2847
2847
  <annotation id="Team286_A510">
2848
2848
  <infon key="type">Gene</infon>
2849
- <location offset="35431" length="5"/>
2849
+ <location offset="35432" length="5"/>
2850
2850
  <text>Lst1p</text>
2851
2851
  </annotation>
2852
2852
  </passage>
@@ -2946,12 +2946,12 @@
2946
2946
  </annotation>
2947
2947
  <annotation id="Team286_A527">
2948
2948
  <infon key="type">Gene</infon>
2949
- <location offset="37960" length="16"/>
2949
+ <location offset="37961" length="16"/>
2950
2950
  <text>lst1Delta mutant</text>
2951
2951
  </annotation>
2952
2952
  <annotation id="Team286_A528">
2953
2953
  <infon key="type">Gene</infon>
2954
- <location offset="38014" length="5"/>
2954
+ <location offset="38015" length="5"/>
2955
2955
  <text>Pma1p</text>
2956
2956
  </annotation>
2957
2957
  </passage>
@@ -3051,37 +3051,37 @@
3051
3051
  </annotation>
3052
3052
  <annotation id="Team286_A545">
3053
3053
  <infon key="type">Gene</infon>
3054
- <location offset="39526" length="25"/>
3054
+ <location offset="39527" length="25"/>
3055
3055
  <text>ER from lst1Delta mutants</text>
3056
3056
  </annotation>
3057
3057
  <annotation id="Team286_A546">
3058
3058
  <infon key="type">Gene</infon>
3059
- <location offset="39564" length="6"/>
3059
+ <location offset="39565" length="6"/>
3060
3060
  <text>Sec61p</text>
3061
3061
  </annotation>
3062
3062
  <annotation id="Team286_A547">
3063
3063
  <infon key="type">Gene</infon>
3064
- <location offset="39664" length="5"/>
3064
+ <location offset="39665" length="5"/>
3065
3065
  <text>Pma1p</text>
3066
3066
  </annotation>
3067
3067
  <annotation id="Team286_A548">
3068
3068
  <infon key="type">Gene</infon>
3069
- <location offset="39771" length="5"/>
3069
+ <location offset="39772" length="5"/>
3070
3070
  <text>Pma1p</text>
3071
3071
  </annotation>
3072
3072
  <annotation id="Team286_A549">
3073
3073
  <infon key="type">Gene</infon>
3074
- <location offset="39918" length="5"/>
3074
+ <location offset="39919" length="5"/>
3075
3075
  <text>Pma1p</text>
3076
3076
  </annotation>
3077
3077
  <annotation id="Team286_A550">
3078
3078
  <infon key="type">Gene</infon>
3079
- <location offset="39976" length="2"/>
3079
+ <location offset="39977" length="2"/>
3080
3080
  <text>ER</text>
3081
3081
  </annotation>
3082
3082
  <annotation id="Team286_A551">
3083
3083
  <infon key="type">Gene</infon>
3084
- <location offset="40013" length="4"/>
3084
+ <location offset="40014" length="4"/>
3085
3085
  <text>PMA1</text>
3086
3086
  </annotation>
3087
3087
  </passage>
@@ -3116,32 +3116,32 @@
3116
3116
  </annotation>
3117
3117
  <annotation id="Team286_A557">
3118
3118
  <infon key="type">Gene</infon>
3119
- <location offset="40289" length="18"/>
3119
+ <location offset="40290" length="18"/>
3120
3120
  <text>lst1Delta mutants</text>
3121
3121
  </annotation>
3122
3122
  <annotation id="Team286_A558">
3123
3123
  <infon key="type">Gene</infon>
3124
- <location offset="40344" length="17"/>
3124
+ <location offset="40345" length="17"/>
3125
3125
  <text>lst1Delta mutants</text>
3126
3126
  </annotation>
3127
3127
  <annotation id="Team286_A559">
3128
3128
  <infon key="type">Gene</infon>
3129
- <location offset="40418" length="9"/>
3129
+ <location offset="40419" length="9"/>
3130
3130
  <text>lst1Delta</text>
3131
3131
  </annotation>
3132
3132
  <annotation id="Team286_A560">
3133
3133
  <infon key="type">Gene</infon>
3134
- <location offset="40661" length="9"/>
3134
+ <location offset="40662" length="9"/>
3135
3135
  <text>invertase</text>
3136
3136
  </annotation>
3137
3137
  <annotation id="Team286_A561">
3138
3138
  <infon key="type">Gene</infon>
3139
- <location offset="40752" length="9"/>
3139
+ <location offset="40753" length="9"/>
3140
3140
  <text>invertase</text>
3141
3141
  </annotation>
3142
3142
  <annotation id="Team286_A562">
3143
3143
  <infon key="type">Gene</infon>
3144
- <location offset="40945" length="18"/>
3144
+ <location offset="40946" length="18"/>
3145
3145
  <text>carboxypeptidase Y</text>
3146
3146
  </annotation>
3147
3147
  </passage>
@@ -3186,17 +3186,17 @@
3186
3186
  </annotation>
3187
3187
  <annotation id="Team286_A570">
3188
3188
  <infon key="type">Gene</infon>
3189
- <location offset="41863" length="10"/>
3189
+ <location offset="41864" length="10"/>
3190
3190
  <text>COPII gene</text>
3191
3191
  </annotation>
3192
3192
  <annotation id="Team286_A571">
3193
3193
  <infon key="type">Gene</infon>
3194
- <location offset="41902" length="9"/>
3194
+ <location offset="41903" length="9"/>
3195
3195
  <text>lst1Delta</text>
3196
3196
  </annotation>
3197
3197
  <annotation id="Team286_A572">
3198
3198
  <infon key="type">Gene</infon>
3199
- <location offset="41958" length="5"/>
3199
+ <location offset="41959" length="5"/>
3200
3200
  <text>Pma1p</text>
3201
3201
  </annotation>
3202
3202
  </passage>
@@ -3301,32 +3301,32 @@
3301
3301
  </annotation>
3302
3302
  <annotation id="Team286_A590">
3303
3303
  <infon key="type">Gene</infon>
3304
- <location offset="43452" length="8"/>
3304
+ <location offset="43453" length="8"/>
3305
3305
  <text>Lst1p-HA</text>
3306
3306
  </annotation>
3307
3307
  <annotation id="Team286_A591">
3308
3308
  <infon key="type">Gene</infon>
3309
- <location offset="43714" length="13"/>
3309
+ <location offset="43715" length="13"/>
3310
3310
  <text>Lst1p protein</text>
3311
3311
  </annotation>
3312
3312
  <annotation id="Team286_A592">
3313
3313
  <infon key="type">Gene</infon>
3314
- <location offset="44000" length="8"/>
3314
+ <location offset="44002" length="8"/>
3315
3315
  <text>Lst1p-HA</text>
3316
3316
  </annotation>
3317
3317
  <annotation id="Team286_A593">
3318
3318
  <infon key="type">Gene</infon>
3319
- <location offset="44037" length="8"/>
3319
+ <location offset="44039" length="8"/>
3320
3320
  <text>Fig. 9 B</text>
3321
3321
  </annotation>
3322
3322
  <annotation id="Team286_A594">
3323
3323
  <infon key="type">Gene</infon>
3324
- <location offset="44077" length="8"/>
3324
+ <location offset="44079" length="8"/>
3325
3325
  <text>Lst1p-HA</text>
3326
3326
  </annotation>
3327
3327
  <annotation id="Team286_A595">
3328
3328
  <infon key="type">Gene</infon>
3329
- <location offset="44148" length="5"/>
3329
+ <location offset="44150" length="5"/>
3330
3330
  <text>Lst1p</text>
3331
3331
  </annotation>
3332
3332
  </passage>
@@ -3346,7 +3346,7 @@
3346
3346
  </annotation>
3347
3347
  <annotation id="TEAM_336_PPI4">
3348
3348
  <infon key="type">PPImention</infon>
3349
- <location offset="44229" length="18"/>
3349
+ <location offset="44229" length="18" original_offset="0"/>
3350
3350
  <text>Lst1p Binds Sec23p</text>
3351
3351
  </annotation>
3352
3352
  </passage>
@@ -3556,12 +3556,12 @@
3556
3556
  </annotation>
3557
3557
  <annotation id="TEAM_336_PPI5">
3558
3558
  <infon key="type">PPImention</infon>
3559
- <location offset="45031" length="120"/>
3559
+ <location offset="45031" length="120" original_offset="0"/>
3560
3560
  <text>To confirm the interaction between Lst1p and Sec23p, association of these proteins was examined in yeast cell extracts.</text>
3561
3561
  </annotation>
3562
3562
  <annotation id="TEAM_336_PPI6">
3563
3563
  <infon key="type">PPImention</infon>
3564
- <location offset="45913" length="90"/>
3564
+ <location offset="45913" length="90" original_offset="0"/>
3565
3565
  <text>Together, these experiments show that Lst1p, like Sec24p, can form a complex with Sec23p.</text>
3566
3566
  </annotation>
3567
3567
  </passage>
@@ -3581,232 +3581,232 @@
3581
3581
  </annotation>
3582
3582
  <annotation id="Team286_A639">
3583
3583
  <infon key="type">Gene</infon>
3584
- <location offset="46173" length="6"/>
3584
+ <location offset="46174" length="6"/>
3585
3585
  <text>Sec23p</text>
3586
3586
  </annotation>
3587
3587
  <annotation id="Team286_A640">
3588
3588
  <infon key="type">Gene</infon>
3589
- <location offset="46189" length="3"/>
3589
+ <location offset="46190" length="3"/>
3590
3590
  <text>GST</text>
3591
3591
  </annotation>
3592
3592
  <annotation id="Team286_A641">
3593
3593
  <infon key="type">Gene</infon>
3594
- <location offset="46193" length="5"/>
3594
+ <location offset="46194" length="5"/>
3595
3595
  <text>Lst1p</text>
3596
3596
  </annotation>
3597
3597
  <annotation id="Team286_A642">
3598
3598
  <infon key="type">Gene</infon>
3599
- <location offset="46199" length="2"/>
3599
+ <location offset="46200" length="2"/>
3600
3600
  <text>HA</text>
3601
3601
  </annotation>
3602
3602
  <annotation id="Team286_A643">
3603
3603
  <infon key="type">Gene</infon>
3604
- <location offset="46237" length="5"/>
3604
+ <location offset="46238" length="5"/>
3605
3605
  <text>Lst1p</text>
3606
3606
  </annotation>
3607
3607
  <annotation id="Team286_A644">
3608
3608
  <infon key="type">Gene</infon>
3609
- <location offset="46243" length="6"/>
3609
+ <location offset="46244" length="6"/>
3610
3610
  <text>Sec23p</text>
3611
3611
  </annotation>
3612
3612
  <annotation id="Team286_A645">
3613
3613
  <infon key="type">Gene</infon>
3614
- <location offset="46343" length="3"/>
3614
+ <location offset="46345" length="3"/>
3615
3615
  <text>GST</text>
3616
3616
  </annotation>
3617
3617
  <annotation id="Team286_A646">
3618
3618
  <infon key="type">Gene</infon>
3619
- <location offset="46347" length="5"/>
3619
+ <location offset="46349" length="5"/>
3620
3620
  <text>Lst1p</text>
3621
3621
  </annotation>
3622
3622
  <annotation id="Team286_A647">
3623
3623
  <infon key="type">Gene</infon>
3624
- <location offset="46353" length="2"/>
3624
+ <location offset="46355" length="2"/>
3625
3625
  <text>HA</text>
3626
3626
  </annotation>
3627
3627
  <annotation id="Team286_A648">
3628
3628
  <infon key="type">Gene</infon>
3629
- <location offset="46360" length="6"/>
3629
+ <location offset="46362" length="6"/>
3630
3630
  <text>Sec23p</text>
3631
3631
  </annotation>
3632
3632
  <annotation id="Team286_A649">
3633
3633
  <infon key="type">Gene</infon>
3634
- <location offset="46416" length="6"/>
3634
+ <location offset="46418" length="6"/>
3635
3635
  <text>Sec23p</text>
3636
3636
  </annotation>
3637
3637
  <annotation id="Team286_A650">
3638
3638
  <infon key="type">Gene</infon>
3639
- <location offset="46440" length="3"/>
3639
+ <location offset="46442" length="3"/>
3640
3640
  <text>GST</text>
3641
3641
  </annotation>
3642
3642
  <annotation id="Team286_A651">
3643
3643
  <infon key="type">Gene</infon>
3644
- <location offset="46444" length="5"/>
3644
+ <location offset="46446" length="5"/>
3645
3645
  <text>Lst1p</text>
3646
3646
  </annotation>
3647
3647
  <annotation id="Team286_A652">
3648
3648
  <infon key="type">Gene</infon>
3649
- <location offset="46450" length="2"/>
3649
+ <location offset="46452" length="2"/>
3650
3650
  <text>HA</text>
3651
3651
  </annotation>
3652
3652
  <annotation id="Team286_A653">
3653
3653
  <infon key="type">Gene</infon>
3654
- <location offset="46573" length="27"/>
3654
+ <location offset="46575" length="27"/>
3655
3655
  <text>ER membrane protein Sec61p</text>
3656
3656
  </annotation>
3657
3657
  <annotation id="Team286_A654">
3658
3658
  <infon key="type">Gene</infon>
3659
- <location offset="46759" length="3"/>
3659
+ <location offset="46761" length="3"/>
3660
3660
  <text>GST</text>
3661
3661
  </annotation>
3662
3662
  <annotation id="Team286_A655">
3663
3663
  <infon key="type">Gene</infon>
3664
- <location offset="46764" length="5"/>
3664
+ <location offset="46766" length="5"/>
3665
3665
  <text>Lst1p</text>
3666
3666
  </annotation>
3667
3667
  <annotation id="Team286_A656">
3668
3668
  <infon key="type">Gene</infon>
3669
- <location offset="46770" length="2"/>
3669
+ <location offset="46772" length="2"/>
3670
3670
  <text>HA</text>
3671
3671
  </annotation>
3672
3672
  <annotation id="Team286_A657">
3673
3673
  <infon key="type">Gene</infon>
3674
- <location offset="46777" length="6"/>
3674
+ <location offset="46779" length="6"/>
3675
3675
  <text>Sec23p</text>
3676
3676
  </annotation>
3677
3677
  <annotation id="Team286_A658">
3678
3678
  <infon key="type">Gene</infon>
3679
- <location offset="46808" length="29"/>
3679
+ <location offset="46810" length="29"/>
3680
3680
  <text>ER resident membrane protein</text>
3681
3681
  </annotation>
3682
3682
  <annotation id="Team286_A659">
3683
3683
  <infon key="type">Gene</infon>
3684
- <location offset="46839" length="6"/>
3684
+ <location offset="46841" length="6"/>
3685
3685
  <text>Sec61p</text>
3686
3686
  </annotation>
3687
3687
  <annotation id="Team286_A660">
3688
3688
  <infon key="type">Gene</infon>
3689
- <location offset="46904" length="3"/>
3689
+ <location offset="46906" length="3"/>
3690
3690
  <text>GST</text>
3691
3691
  </annotation>
3692
3692
  <annotation id="Team286_A661">
3693
3693
  <infon key="type">Gene</infon>
3694
- <location offset="46908" length="5"/>
3694
+ <location offset="46910" length="5"/>
3695
3695
  <text>Lst1p</text>
3696
3696
  </annotation>
3697
3697
  <annotation id="Team286_A662">
3698
3698
  <infon key="type">Gene</infon>
3699
- <location offset="46914" length="2"/>
3699
+ <location offset="46916" length="2"/>
3700
3700
  <text>HA</text>
3701
3701
  </annotation>
3702
3702
  <annotation id="Team286_A663">
3703
3703
  <infon key="type">Gene</infon>
3704
- <location offset="46921" length="6"/>
3704
+ <location offset="46923" length="6"/>
3705
3705
  <text>Sec23p</text>
3706
3706
  </annotation>
3707
3707
  <annotation id="Team286_A664">
3708
3708
  <infon key="type">Gene</infon>
3709
- <location offset="47007" length="6"/>
3709
+ <location offset="47009" length="6"/>
3710
3710
  <text>Sec23p</text>
3711
3711
  </annotation>
3712
3712
  <annotation id="Team286_A665">
3713
3713
  <infon key="type">Gene</infon>
3714
- <location offset="47018" length="3"/>
3714
+ <location offset="47020" length="3"/>
3715
3715
  <text>GST</text>
3716
3716
  </annotation>
3717
3717
  <annotation id="Team286_A666">
3718
3718
  <infon key="type">Gene</infon>
3719
- <location offset="47022" length="5"/>
3719
+ <location offset="47024" length="5"/>
3720
3720
  <text>Lst1p</text>
3721
3721
  </annotation>
3722
3722
  <annotation id="Team286_A667">
3723
3723
  <infon key="type">Gene</infon>
3724
- <location offset="47028" length="2"/>
3724
+ <location offset="47030" length="2"/>
3725
3725
  <text>HA</text>
3726
3726
  </annotation>
3727
3727
  <annotation id="Team286_A668">
3728
3728
  <infon key="type">Gene</infon>
3729
- <location offset="47069" length="6"/>
3729
+ <location offset="47071" length="6"/>
3730
3730
  <text>Sec23p</text>
3731
3731
  </annotation>
3732
3732
  <annotation id="Team286_A669">
3733
3733
  <infon key="type">Gene</infon>
3734
- <location offset="47138" length="6"/>
3734
+ <location offset="47140" length="6"/>
3735
3735
  <text>Sec23p</text>
3736
3736
  </annotation>
3737
3737
  <annotation id="Team286_A670">
3738
3738
  <infon key="type">Gene</infon>
3739
- <location offset="47175" length="3"/>
3739
+ <location offset="47177" length="3"/>
3740
3740
  <text>GST</text>
3741
3741
  </annotation>
3742
3742
  <annotation id="Team286_A671">
3743
3743
  <infon key="type">Gene</infon>
3744
- <location offset="47179" length="5"/>
3744
+ <location offset="47181" length="5"/>
3745
3745
  <text>Lst1p</text>
3746
3746
  </annotation>
3747
3747
  <annotation id="Team286_A672">
3748
3748
  <infon key="type">Gene</infon>
3749
- <location offset="47185" length="2"/>
3749
+ <location offset="47187" length="2"/>
3750
3750
  <text>HA</text>
3751
3751
  </annotation>
3752
3752
  <annotation id="Team286_A673">
3753
3753
  <infon key="type">Gene</infon>
3754
- <location offset="47246" length="3"/>
3754
+ <location offset="47248" length="3"/>
3755
3755
  <text>GST</text>
3756
3756
  </annotation>
3757
3757
  <annotation id="Team286_A674">
3758
3758
  <infon key="type">Gene</infon>
3759
- <location offset="47250" length="5"/>
3759
+ <location offset="47252" length="5"/>
3760
3760
  <text>Lst1p</text>
3761
3761
  </annotation>
3762
3762
  <annotation id="Team286_A675">
3763
3763
  <infon key="type">Gene</infon>
3764
- <location offset="47297" length="6"/>
3764
+ <location offset="47299" length="6"/>
3765
3765
  <text>Sec23p</text>
3766
3766
  </annotation>
3767
3767
  <annotation id="Team286_A676">
3768
3768
  <infon key="type">Gene</infon>
3769
- <location offset="47307" length="3"/>
3769
+ <location offset="47309" length="3"/>
3770
3770
  <text>GST</text>
3771
3771
  </annotation>
3772
3772
  <annotation id="Team286_A677">
3773
3773
  <infon key="type">Gene</infon>
3774
- <location offset="47311" length="5"/>
3774
+ <location offset="47313" length="5"/>
3775
3775
  <text>Lst1p</text>
3776
3776
  </annotation>
3777
3777
  <annotation id="Team286_A678">
3778
3778
  <infon key="type">Gene</infon>
3779
- <location offset="47317" length="2"/>
3779
+ <location offset="47319" length="2"/>
3780
3780
  <text>HA</text>
3781
3781
  </annotation>
3782
3782
  <annotation id="Team286_A679">
3783
3783
  <infon key="type">Gene</infon>
3784
- <location offset="47541" length="5"/>
3784
+ <location offset="47543" length="5"/>
3785
3785
  <text>Lst1p</text>
3786
3786
  </annotation>
3787
3787
  <annotation id="Team286_A680">
3788
3788
  <infon key="type">Gene</infon>
3789
- <location offset="47572" length="6"/>
3789
+ <location offset="47574" length="6"/>
3790
3790
  <text>Sec23p</text>
3791
3791
  </annotation>
3792
3792
  <annotation id="Team286_A681">
3793
3793
  <infon key="type">Gene</infon>
3794
- <location offset="47593" length="5"/>
3794
+ <location offset="47595" length="5"/>
3795
3795
  <text>Lst1p</text>
3796
3796
  </annotation>
3797
3797
  <annotation id="Team286_A682">
3798
3798
  <infon key="type">Gene</infon>
3799
- <location offset="47600" length="6"/>
3799
+ <location offset="47602" length="6"/>
3800
3800
  <text>Sec23p</text>
3801
3801
  </annotation>
3802
3802
  <annotation id="TEAM_336_PPI7">
3803
3803
  <infon key="type">PPImention</infon>
3804
- <location offset="46119" length="213"/>
3804
+ <location offset="46120" length="213" original_offset="0"/>
3805
3805
  <text>While working out conditions to optimize recovery of Sec23p bound to GST-Lst1p-HA, we discovered that assembly of an Lst1p/Sec23p complex appears to enhance the association of both proteins with the ER membrane.</text>
3806
3806
  </annotation>
3807
3807
  <annotation id="TEAM_336_PPI8">
3808
3808
  <infon key="type">PPImention</infon>
3809
- <location offset="47501" length="144"/>
3809
+ <location offset="47503" length="144" original_offset="0"/>
3810
3810
  <text>These data support the observation that Lst1p can form a complex with Sec23p, and that the Lst1p/ Sec23p complex has affinity for ER membranes.</text>
3811
3811
  </annotation>
3812
3812
  </passage>
@@ -3946,62 +3946,62 @@
3946
3946
  </annotation>
3947
3947
  <annotation id="Team286_A708">
3948
3948
  <infon key="type">Gene</infon>
3949
- <location offset="48914" length="6"/>
3949
+ <location offset="48915" length="6"/>
3950
3950
  <text>Sec23p</text>
3951
3951
  </annotation>
3952
3952
  <annotation id="Team286_A709">
3953
3953
  <infon key="type">Gene</infon>
3954
- <location offset="48921" length="5"/>
3954
+ <location offset="48922" length="5"/>
3955
3955
  <text>Lst1p</text>
3956
3956
  </annotation>
3957
3957
  <annotation id="Team286_A710">
3958
3958
  <infon key="type">Gene</infon>
3959
- <location offset="48988" length="5"/>
3959
+ <location offset="48989" length="5"/>
3960
3960
  <text>Lst1p</text>
3961
3961
  </annotation>
3962
3962
  <annotation id="Team286_A711">
3963
3963
  <infon key="type">Gene</infon>
3964
- <location offset="48999" length="6"/>
3964
+ <location offset="49000" length="6"/>
3965
3965
  <text>Sec23p</text>
3966
3966
  </annotation>
3967
3967
  <annotation id="Team286_A712">
3968
3968
  <infon key="type">Gene</infon>
3969
- <location offset="49202" length="4"/>
3969
+ <location offset="49203" length="4"/>
3970
3970
  <text>LST1</text>
3971
3971
  </annotation>
3972
3972
  <annotation id="Team286_A713">
3973
3973
  <infon key="type">Gene</infon>
3974
- <location offset="49319" length="5"/>
3974
+ <location offset="49320" length="5"/>
3975
3975
  <text>Pma1p</text>
3976
3976
  </annotation>
3977
3977
  <annotation id="Team286_A714">
3978
3978
  <infon key="type">Gene</infon>
3979
- <location offset="49333" length="2"/>
3979
+ <location offset="49334" length="2"/>
3980
3980
  <text>ER</text>
3981
3981
  </annotation>
3982
3982
  <annotation id="Team286_A715">
3983
3983
  <infon key="type">Gene</infon>
3984
- <location offset="49432" length="5"/>
3984
+ <location offset="49434" length="5"/>
3985
3985
  <text>Lst1p</text>
3986
3986
  </annotation>
3987
3987
  <annotation id="Team286_A716">
3988
3988
  <infon key="type">Gene</infon>
3989
- <location offset="49458" length="6"/>
3989
+ <location offset="49460" length="6"/>
3990
3990
  <text>Sec24p</text>
3991
3991
  </annotation>
3992
3992
  <annotation id="Team286_A717">
3993
3993
  <infon key="type">Gene</infon>
3994
- <location offset="49482" length="18"/>
3994
+ <location offset="49484" length="18"/>
3995
3995
  <text>COPII coat complex</text>
3996
3996
  </annotation>
3997
3997
  <annotation id="Team286_A718">
3998
3998
  <infon key="type">Gene</infon>
3999
- <location offset="49538" length="5"/>
3999
+ <location offset="49540" length="5"/>
4000
4000
  <text>Pma1p</text>
4001
4001
  </annotation>
4002
4002
  <annotation id="TEAM_336_PPI9">
4003
4003
  <infon key="type">PPImention</infon>
4004
- <location offset="48738" length="154"/>
4004
+ <location offset="48738" length="154" original_offset="0"/>
4005
4005
  <text>(III) Lst1p, like Sec24p, can bind to Sec23p as shown by tests for two-hybrid interaction and affinity purification of a complex of GST-Lst1p and Sec23p.</text>
4006
4006
  </annotation>
4007
4007
  </passage>
@@ -4426,32 +4426,32 @@
4426
4426
  </annotation>
4427
4427
  <annotation id="Team286_A796">
4428
4428
  <infon key="type">Gene</infon>
4429
- <location offset="55725" length="5"/>
4429
+ <location offset="55726" length="5"/>
4430
4430
  <text>Lst1p</text>
4431
4431
  </annotation>
4432
4432
  <annotation id="Team286_A797">
4433
4433
  <infon key="type">Gene</infon>
4434
- <location offset="55760" length="6"/>
4434
+ <location offset="55761" length="6"/>
4435
4435
  <text>Sec24p</text>
4436
4436
  </annotation>
4437
4437
  <annotation id="Team286_A798">
4438
4438
  <infon key="type">Gene</infon>
4439
- <location offset="55800" length="5"/>
4439
+ <location offset="55801" length="5"/>
4440
4440
  <text>Pma1p</text>
4441
4441
  </annotation>
4442
4442
  <annotation id="Team286_A799">
4443
4443
  <infon key="type">Gene</infon>
4444
- <location offset="55845" length="12"/>
4444
+ <location offset="55847" length="12"/>
4445
4445
  <text>Sec24 family</text>
4446
4446
  </annotation>
4447
4447
  <annotation id="TEAM_336_PPI10">
4448
4448
  <infon key="type">PPImention</infon>
4449
- <location offset="55027" length="257"/>
4449
+ <location offset="55027" length="257" original_offset="0"/>
4450
4450
  <text>Using ER-derived microsomes and purified COPII components, Kuehn et al. (1998) have shown that the Sec23p/Sec24p complex, along with Sar1p, associate with amino acid permeases and other integral membrane protein that are destined for the plasma membrane.</text>
4451
4451
  </annotation>
4452
4452
  <annotation id="TEAM_336_PPI11">
4453
4453
  <infon key="type">PPImention</infon>
4454
- <location offset="55498" length="192"/>
4454
+ <location offset="55498" length="192" original_offset="0"/>
4455
4455
  <text>The conclusion from both experimental systems is that the Sec23p/Sec24p complex contains specific binding sites for the capture of membrane cargo proteins within the plane of the ER membrane.</text>
4456
4456
  </annotation>
4457
4457
  </passage>
@@ -4531,7 +4531,7 @@
4531
4531
  </annotation>
4532
4532
  <annotation id="TEAM_336_PPI12">
4533
4533
  <infon key="type">PPImention</infon>
4534
- <location offset="56337" length="495"/>
4534
+ <location offset="56337" length="495" original_offset="0"/>
4535
4535
  <text>In future experiments, it may be possible to isolate vesicles coated with Lst1p by performing an in vitro budding reaction using purified cytosolic components, including a purified complex of Lst1p and Sec23p. It may also be possible to determine whether vesicles that are formed using a Sec23p/Lst1p complex more efficiently incorporate Pma1p than vesicles formed using the Sec23p/Sec24p complex. Finally, it will be of interest to determine if there is direct binding of Lst1p to Pma1p.</text>
4536
4536
  </annotation>
4537
4537
  </passage>
@@ -4626,12 +4626,12 @@
4626
4626
  </annotation>
4627
4627
  <annotation id="TEAM_336_PPI13">
4628
4628
  <infon key="type">PPImention</infon>
4629
- <location offset="56999" length="139"/>
4629
+ <location offset="56999" length="139" original_offset="0"/>
4630
4630
  <text>It is possible that Sec23p/Lst1p complexes act to form a class of vesicle that is distinct from those formed by Sec23p/ Sec24p complexes.</text>
4631
4631
  </annotation>
4632
4632
  <annotation id="TEAM_336_PPI14">
4633
4633
  <infon key="type">PPImention</infon>
4634
- <location offset="57403" length="208"/>
4634
+ <location offset="57403" length="208" original_offset="0"/>
4635
4635
  <text>We have identified a third Sec24p family member, which we call Iss1p, as a protein that binds to Sec16p. Iss1p (YNL049c) also binds Sec23p and appears to be associated with the ER membrane (Gimeno, 1996).</text>
4636
4636
  </annotation>
4637
4637
  </passage>
@@ -4651,297 +4651,297 @@
4651
4651
  <text>Strain Genotype Source or reference CUY563 MATaade2-101 ade3-24 leu2-3,112 ura3-52 T. Huffaker (Cornell University) CUY564 MATalpha ade2-101 ade3-2 leu2-3,112 ura3-52 T. Huffaker (Cornell University) EGY40 MATalpha ura3-52 leu2 his3 trp1 Golemis and Brent, 1992 CKY45 MATalpha sec13-1 his4-619 ura3-52 Kaiser Lab Collection CKY50 MATalpha sec16-2 his4-619 ura3-52 Kaiser Lab Collection CKY54 MATalpha sec17-1 his4-619 ura3-52 Kaiser Lab Collection CKY58 MATalpha sec18-1 his4-619 ura3-52 Kaiser Lab Collection CKY78 MATalpha sec23-1 his4-619 ura3-52 Kaiser Lab Collection CKY348 MATa/alpha leu2-3/leu2-3 ura3-52/ura3-52 Kaiser Lab Collection CKY423 MATalpha sec13-1 ade2-101 ade3-24 leu2-3,112 ura3-52 [pKR4] CKY424 MATasec13-1 ade2-101 ade3-24 leu2-3,112 ura3-52 [pKR4] CKY426 MATalst1-1sec13-1 ade2-101 ade3-24 leu2-3,112 ura3-52 [pKR4] CKY435 MATalst1-1 sec13-1::[SEC13, URA3] ade2-101 ade3-24 leu2-3,112 ura3-52 CKY436 MATalst2-1 sec13-1::[SEC13, URA3] ade2-101 ade3-24 leu2-3,112 ura3-52 CKY437 MATalst3-1 sec13-1::[SEC13, URA3] ade2-101 ade3-24 leu2-3,112 ura3-52 CKY438 MATalst4-1 sec13-1::[SEC13, URA3] ade2-101 ade3-24 leu2-3,112 ura3-52 CKY439 MATalst5-1 sec13-1::[SEC13, URA3] ade2-101 ade3-24 leu2-3,112 ura3-52 CKY440 MATalst6-1 sec13-1::[SEC13, URA3] ade2-101 ade3-24 leu2-3,112 ura3-52 CKY441 MATalst7-1 sec13-1::[SEC13, URA3] ade2-101 ade3-24 leu2-3,112 ura3-52 CKY442 MATalst8-1 sec13-1::[SEC13, URA3] ade2-101 ade3-24 leu2-3,112 ura3-52 CKY443 MATa prototroph Kaiser Lab Collection CKY473 MATaleu2-3,112 ura3-52 Gal+ Kaiser Lab Collection CKY534 MATalpha lst1Delta::LEU2 leu2-3,112 ura3-52 [pKR17HA] CKY535 MATalst1Delta::LEU2 leu2-3,112 ura3-52 [pKR17HA] CKY536 MATalst1Delta::LEU2 ura3-52 leu2-3,112 CKY540 MATaleu2-3,112 ura3-52 [pNV31] CKY541 MATasec12-4 ura3-52 [pNV31] CKY542 MATalst1Delta::LEU2 leu2-3,112 ura3-52 [pNV31] CKY552 MATalpha lst1Delta::LEU2 leu2-3,112 ura3-52</text>
4652
4652
  <annotation id="Team286_A831">
4653
4653
  <infon key="type">Gene</infon>
4654
- <location offset="58193" length="35"/>
4654
+ <location offset="58196" length="35"/>
4655
4655
  <text>CUY563 MATaade2-101 ade3-24 leu2-3</text>
4656
4656
  </annotation>
4657
4657
  <annotation id="Team286_A832">
4658
4658
  <infon key="type">Gene</infon>
4659
- <location offset="58284" length="31"/>
4659
+ <location offset="58287" length="31"/>
4660
4660
  <text>MATalpha ade2-101 ade3-2 leu2-3</text>
4661
4661
  </annotation>
4662
4662
  <annotation id="Team286_A833">
4663
4663
  <infon key="type">Gene</infon>
4664
- <location offset="58370" length="175"/>
4664
+ <location offset="58373" length="175"/>
4665
4665
  <text>MATalpha ura3-52 leu2 his3 trp1 Golemis and Brent, 1992 CKY45 MATalpha sec13-1 his4-619 ura3-52 Kaiser Lab Collection CKY50 MATalpha sec16-2 his4-619 ura3-52 Kaiser Lab</text>
4666
4666
  </annotation>
4667
4667
  <annotation id="Team286_A834">
4668
4668
  <infon key="type">Gene</infon>
4669
- <location offset="58558" length="52"/>
4669
+ <location offset="58561" length="52"/>
4670
4670
  <text>CKY54 MATalpha sec17-1 his4-619 ura3-52 Kaiser Lab</text>
4671
4671
  </annotation>
4672
4672
  <annotation id="Team286_A835">
4673
4673
  <infon key="type">Gene</infon>
4674
- <location offset="58623" length="117"/>
4674
+ <location offset="58626" length="117"/>
4675
4675
  <text>CKY58 MATalpha sec18-1 his4-619 ura3-52 Kaiser Lab Collection CKY78 MATalpha sec23-1 his4-619 ura3-52 Kaiser Lab</text>
4676
4676
  </annotation>
4677
4677
  <annotation id="Team286_A836">
4678
4678
  <infon key="type">Gene</infon>
4679
- <location offset="58753" length="25"/>
4679
+ <location offset="58756" length="25"/>
4680
4680
  <text>CKY348 MATa/alpha leu2-3</text>
4681
4681
  </annotation>
4682
4682
  <annotation id="Team286_A837">
4683
4683
  <infon key="type">Gene</infon>
4684
- <location offset="58779" length="14"/>
4684
+ <location offset="58782" length="14"/>
4685
4685
  <text>leu2-3 ura3-52</text>
4686
4686
  </annotation>
4687
4687
  <annotation id="Team286_A838">
4688
4688
  <infon key="type">Gene</infon>
4689
- <location offset="58794" length="158"/>
4689
+ <location offset="58797" length="158"/>
4690
4690
  <text>ura3-52 Kaiser Lab Collection CKY423 MATalpha sec13-1 ade2-101 ade3-24 leu2-3,112 ura3-52 [pKR4] CKY424 MATasec13-1 ade2-101 ade3-24 leu2-3,112 ura3-52</text>
4691
4691
  </annotation>
4692
4692
  <annotation id="Team286_A839">
4693
4693
  <infon key="type">Gene</infon>
4694
- <location offset="58954" length="4"/>
4694
+ <location offset="58957" length="4"/>
4695
4695
  <text>pKR4</text>
4696
4696
  </annotation>
4697
4697
  <annotation id="Team286_A840">
4698
4698
  <infon key="type">Gene</infon>
4699
- <location offset="58963" length="61"/>
4699
+ <location offset="58966" length="61"/>
4700
4700
  <text>CKY426 MATalst1-1sec13-1 ade2-101 ade3-24 leu2-3,112 ura3-52</text>
4701
4701
  </annotation>
4702
4702
  <annotation id="Team286_A841">
4703
4703
  <infon key="type">Gene</infon>
4704
- <location offset="59026" length="4"/>
4704
+ <location offset="59029" length="4"/>
4705
4705
  <text>pKR4</text>
4706
4706
  </annotation>
4707
4707
  <annotation id="Team286_A842">
4708
4708
  <infon key="type">Gene</infon>
4709
- <location offset="59035" length="26"/>
4709
+ <location offset="59038" length="26"/>
4710
4710
  <text>CKY435 MATalst1-1 sec13-1</text>
4711
4711
  </annotation>
4712
4712
  <annotation id="Team286_A843">
4713
4713
  <infon key="type">Gene</infon>
4714
- <location offset="59064" length="5"/>
4714
+ <location offset="59067" length="5"/>
4715
4715
  <text>SEC13</text>
4716
4716
  </annotation>
4717
4717
  <annotation id="Team286_A844">
4718
4718
  <infon key="type">Gene</infon>
4719
- <location offset="59071" length="4"/>
4719
+ <location offset="59074" length="4"/>
4720
4720
  <text>URA3</text>
4721
4721
  </annotation>
4722
4722
  <annotation id="Team286_A845">
4723
4723
  <infon key="type">Gene</infon>
4724
- <location offset="59077" length="23"/>
4724
+ <location offset="59080" length="23"/>
4725
4725
  <text>ade2-101 ade3-24 leu2-3</text>
4726
4726
  </annotation>
4727
4727
  <annotation id="Team286_A846">
4728
4728
  <infon key="type">Gene</infon>
4729
- <location offset="59101" length="3"/>
4729
+ <location offset="59104" length="3"/>
4730
4730
  <text>112</text>
4731
4731
  </annotation>
4732
4732
  <annotation id="Team286_A847">
4733
4733
  <infon key="type">Gene</infon>
4734
- <location offset="59105" length="37"/>
4734
+ <location offset="59108" length="37"/>
4735
4735
  <text>ura3-52 CKY436 MATalst2-1 sec13-1</text>
4736
4736
  </annotation>
4737
4737
  <annotation id="Team286_A848">
4738
4738
  <infon key="type">Gene</infon>
4739
- <location offset="59145" length="5"/>
4739
+ <location offset="59148" length="5"/>
4740
4740
  <text>SEC13</text>
4741
4741
  </annotation>
4742
4742
  <annotation id="Team286_A849">
4743
4743
  <infon key="type">Gene</infon>
4744
- <location offset="59152" length="4"/>
4744
+ <location offset="59155" length="4"/>
4745
4745
  <text>URA3</text>
4746
4746
  </annotation>
4747
4747
  <annotation id="Team286_A850">
4748
4748
  <infon key="type">Gene</infon>
4749
- <location offset="59158" length="23"/>
4749
+ <location offset="59161" length="23"/>
4750
4750
  <text>ade2-101 ade3-24 leu2-3</text>
4751
4751
  </annotation>
4752
4752
  <annotation id="Team286_A851">
4753
4753
  <infon key="type">Gene</infon>
4754
- <location offset="59182" length="3"/>
4754
+ <location offset="59185" length="3"/>
4755
4755
  <text>112</text>
4756
4756
  </annotation>
4757
4757
  <annotation id="Team286_A852">
4758
4758
  <infon key="type">Gene</infon>
4759
- <location offset="59186" length="37"/>
4759
+ <location offset="59189" length="37"/>
4760
4760
  <text>ura3-52 CKY437 MATalst3-1 sec13-1</text>
4761
4761
  </annotation>
4762
4762
  <annotation id="Team286_A853">
4763
4763
  <infon key="type">Gene</infon>
4764
- <location offset="59226" length="5"/>
4764
+ <location offset="59229" length="5"/>
4765
4765
  <text>SEC13</text>
4766
4766
  </annotation>
4767
4767
  <annotation id="Team286_A854">
4768
4768
  <infon key="type">Gene</infon>
4769
- <location offset="59233" length="4"/>
4769
+ <location offset="59236" length="4"/>
4770
4770
  <text>URA3</text>
4771
4771
  </annotation>
4772
4772
  <annotation id="Team286_A855">
4773
4773
  <infon key="type">Gene</infon>
4774
- <location offset="59239" length="23"/>
4774
+ <location offset="59242" length="23"/>
4775
4775
  <text>ade2-101 ade3-24 leu2-3</text>
4776
4776
  </annotation>
4777
4777
  <annotation id="Team286_A856">
4778
4778
  <infon key="type">Gene</infon>
4779
- <location offset="59263" length="3"/>
4779
+ <location offset="59266" length="3"/>
4780
4780
  <text>112</text>
4781
4781
  </annotation>
4782
4782
  <annotation id="Team286_A857">
4783
4783
  <infon key="type">Gene</infon>
4784
- <location offset="59267" length="37"/>
4784
+ <location offset="59270" length="37"/>
4785
4785
  <text>ura3-52 CKY438 MATalst4-1 sec13-1</text>
4786
4786
  </annotation>
4787
4787
  <annotation id="Team286_A858">
4788
4788
  <infon key="type">Gene</infon>
4789
- <location offset="59307" length="5"/>
4789
+ <location offset="59310" length="5"/>
4790
4790
  <text>SEC13</text>
4791
4791
  </annotation>
4792
4792
  <annotation id="Team286_A859">
4793
4793
  <infon key="type">Gene</infon>
4794
- <location offset="59314" length="4"/>
4794
+ <location offset="59317" length="4"/>
4795
4795
  <text>URA3</text>
4796
4796
  </annotation>
4797
4797
  <annotation id="Team286_A860">
4798
4798
  <infon key="type">Gene</infon>
4799
- <location offset="59320" length="23"/>
4799
+ <location offset="59323" length="23"/>
4800
4800
  <text>ade2-101 ade3-24 leu2-3</text>
4801
4801
  </annotation>
4802
4802
  <annotation id="Team286_A861">
4803
4803
  <infon key="type">Gene</infon>
4804
- <location offset="59344" length="3"/>
4804
+ <location offset="59347" length="3"/>
4805
4805
  <text>112</text>
4806
4806
  </annotation>
4807
4807
  <annotation id="Team286_A862">
4808
4808
  <infon key="type">Gene</infon>
4809
- <location offset="59348" length="37"/>
4809
+ <location offset="59351" length="37"/>
4810
4810
  <text>ura3-52 CKY439 MATalst5-1 sec13-1</text>
4811
4811
  </annotation>
4812
4812
  <annotation id="Team286_A863">
4813
4813
  <infon key="type">Gene</infon>
4814
- <location offset="59388" length="5"/>
4814
+ <location offset="59391" length="5"/>
4815
4815
  <text>SEC13</text>
4816
4816
  </annotation>
4817
4817
  <annotation id="Team286_A864">
4818
4818
  <infon key="type">Gene</infon>
4819
- <location offset="59395" length="4"/>
4819
+ <location offset="59398" length="4"/>
4820
4820
  <text>URA3</text>
4821
4821
  </annotation>
4822
4822
  <annotation id="Team286_A865">
4823
4823
  <infon key="type">Gene</infon>
4824
- <location offset="59401" length="23"/>
4824
+ <location offset="59404" length="23"/>
4825
4825
  <text>ade2-101 ade3-24 leu2-3</text>
4826
4826
  </annotation>
4827
4827
  <annotation id="Team286_A866">
4828
4828
  <infon key="type">Gene</infon>
4829
- <location offset="59425" length="3"/>
4829
+ <location offset="59428" length="3"/>
4830
4830
  <text>112</text>
4831
4831
  </annotation>
4832
4832
  <annotation id="Team286_A867">
4833
4833
  <infon key="type">Gene</infon>
4834
- <location offset="59429" length="37"/>
4834
+ <location offset="59432" length="37"/>
4835
4835
  <text>ura3-52 CKY440 MATalst6-1 sec13-1</text>
4836
4836
  </annotation>
4837
4837
  <annotation id="Team286_A868">
4838
4838
  <infon key="type">Gene</infon>
4839
- <location offset="59469" length="5"/>
4839
+ <location offset="59472" length="5"/>
4840
4840
  <text>SEC13</text>
4841
4841
  </annotation>
4842
4842
  <annotation id="Team286_A869">
4843
4843
  <infon key="type">Gene</infon>
4844
- <location offset="59476" length="4"/>
4844
+ <location offset="59479" length="4"/>
4845
4845
  <text>URA3</text>
4846
4846
  </annotation>
4847
4847
  <annotation id="Team286_A870">
4848
4848
  <infon key="type">Gene</infon>
4849
- <location offset="59482" length="23"/>
4849
+ <location offset="59485" length="23"/>
4850
4850
  <text>ade2-101 ade3-24 leu2-3</text>
4851
4851
  </annotation>
4852
4852
  <annotation id="Team286_A871">
4853
4853
  <infon key="type">Gene</infon>
4854
- <location offset="59506" length="3"/>
4854
+ <location offset="59509" length="3"/>
4855
4855
  <text>112</text>
4856
4856
  </annotation>
4857
4857
  <annotation id="Team286_A872">
4858
4858
  <infon key="type">Gene</infon>
4859
- <location offset="59510" length="37"/>
4859
+ <location offset="59513" length="37"/>
4860
4860
  <text>ura3-52 CKY441 MATalst7-1 sec13-1</text>
4861
4861
  </annotation>
4862
4862
  <annotation id="Team286_A873">
4863
4863
  <infon key="type">Gene</infon>
4864
- <location offset="59550" length="5"/>
4864
+ <location offset="59553" length="5"/>
4865
4865
  <text>SEC13</text>
4866
4866
  </annotation>
4867
4867
  <annotation id="Team286_A874">
4868
4868
  <infon key="type">Gene</infon>
4869
- <location offset="59557" length="4"/>
4869
+ <location offset="59560" length="4"/>
4870
4870
  <text>URA3</text>
4871
4871
  </annotation>
4872
4872
  <annotation id="Team286_A875">
4873
4873
  <infon key="type">Gene</infon>
4874
- <location offset="59563" length="23"/>
4874
+ <location offset="59566" length="23"/>
4875
4875
  <text>ade2-101 ade3-24 leu2-3</text>
4876
4876
  </annotation>
4877
4877
  <annotation id="Team286_A876">
4878
4878
  <infon key="type">Gene</infon>
4879
- <location offset="59587" length="3"/>
4879
+ <location offset="59590" length="3"/>
4880
4880
  <text>112</text>
4881
4881
  </annotation>
4882
4882
  <annotation id="Team286_A877">
4883
4883
  <infon key="type">Gene</infon>
4884
- <location offset="59591" length="37"/>
4884
+ <location offset="59594" length="37"/>
4885
4885
  <text>ura3-52 CKY442 MATalst8-1 sec13-1</text>
4886
4886
  </annotation>
4887
4887
  <annotation id="Team286_A878">
4888
4888
  <infon key="type">Gene</infon>
4889
- <location offset="59631" length="5"/>
4889
+ <location offset="59634" length="5"/>
4890
4890
  <text>SEC13</text>
4891
4891
  </annotation>
4892
4892
  <annotation id="Team286_A879">
4893
4893
  <infon key="type">Gene</infon>
4894
- <location offset="59638" length="4"/>
4894
+ <location offset="59641" length="4"/>
4895
4895
  <text>URA3</text>
4896
4896
  </annotation>
4897
4897
  <annotation id="Team286_A880">
4898
4898
  <infon key="type">Gene</infon>
4899
- <location offset="59644" length="23"/>
4899
+ <location offset="59647" length="23"/>
4900
4900
  <text>ade2-101 ade3-24 leu2-3</text>
4901
4901
  </annotation>
4902
4902
  <annotation id="Team286_A881">
4903
4903
  <infon key="type">Gene</infon>
4904
- <location offset="59668" length="3"/>
4904
+ <location offset="59671" length="3"/>
4905
4905
  <text>112</text>
4906
4906
  </annotation>
4907
4907
  <annotation id="Team286_A882">
4908
4908
  <infon key="type">Gene</infon>
4909
- <location offset="59672" length="145"/>
4909
+ <location offset="59675" length="145"/>
4910
4910
  <text>ura3-52 CKY443 MATa prototroph Kaiser Lab Collection CKY473 MATaleu2-3,112 ura3-52 Gal+ Kaiser Lab Collection CKY534 MATalpha lst1Delta</text>
4911
4911
  </annotation>
4912
4912
  <annotation id="Team286_A883">
4913
4913
  <infon key="type">Gene</infon>
4914
- <location offset="59819" length="58"/>
4914
+ <location offset="59822" length="58"/>
4915
4915
  <text>LEU2 leu2-3,112 ura3-52 [pKR17HA] CKY535 MATalst1Delta</text>
4916
4916
  </annotation>
4917
4917
  <annotation id="Team286_A884">
4918
4918
  <infon key="type">Gene</infon>
4919
- <location offset="59879" length="58"/>
4919
+ <location offset="59882" length="58"/>
4920
4920
  <text>LEU2 leu2-3,112 ura3-52 [pKR17HA] CKY536 MATalst1Delta</text>
4921
4921
  </annotation>
4922
4922
  <annotation id="Team286_A885">
4923
4923
  <infon key="type">Gene</infon>
4924
- <location offset="59939" length="129"/>
4924
+ <location offset="59942" length="129"/>
4925
4925
  <text>LEU2 ura3-52 leu2-3,112 CKY540 MATaleu2-3,112 ura3-52 [pNV31] CKY541 MATasec12-4 ura3-52 [pNV31] CKY542 MATalst1Delta</text>
4926
4926
  </annotation>
4927
4927
  <annotation id="Team286_A886">
4928
4928
  <infon key="type">Gene</infon>
4929
- <location offset="60070" length="61"/>
4929
+ <location offset="60073" length="61"/>
4930
4930
  <text>LEU2 leu2-3,112 ura3-52 [pNV31] CKY552 MATalpha lst1Delta</text>
4931
4931
  </annotation>
4932
4932
  <annotation id="Team286_A887">
4933
4933
  <infon key="type">Gene</infon>
4934
- <location offset="60133" length="11"/>
4934
+ <location offset="60136" length="11"/>
4935
4935
  <text>LEU2 leu2-3</text>
4936
4936
  </annotation>
4937
4937
  <annotation id="Team286_A888">
4938
4938
  <infon key="type">Gene</infon>
4939
- <location offset="60145" length="3"/>
4939
+ <location offset="60148" length="3"/>
4940
4940
  <text>112</text>
4941
4941
  </annotation>
4942
4942
  <annotation id="Team286_A889">
4943
4943
  <infon key="type">Gene</infon>
4944
- <location offset="60149" length="7"/>
4944
+ <location offset="60152" length="7"/>
4945
4945
  <text>ura3-52</text>
4946
4946
  </annotation>
4947
4947
  </passage>
@@ -5016,17 +5016,17 @@
5016
5016
  <text>Gene Number of alleles LST1 11 LST2 6 LST3 4 LST4 5 LST5 5 LST6 1 LST7 1 LST8 1 LST9 1 LST10 (SEC16) 2</text>
5017
5017
  <annotation id="Team286_A900">
5018
5018
  <infon key="type">Gene</infon>
5019
- <location offset="60755" length="14"/>
5019
+ <location offset="60758" length="14"/>
5020
5020
  <text>LST1 11 LST2</text>
5021
5021
  </annotation>
5022
5022
  <annotation id="Team286_A901">
5023
5023
  <infon key="type">Gene</infon>
5024
- <location offset="60775" length="75"/>
5024
+ <location offset="60778" length="75"/>
5025
5025
  <text>LST3 4 LST4 5 LST5 5 LST6 1 LST7 1 LST8 1 LST9 1 LST10</text>
5026
5026
  </annotation>
5027
5027
  <annotation id="Team286_A902">
5028
5028
  <infon key="type">Gene</infon>
5029
- <location offset="60852" length="5"/>
5029
+ <location offset="60855" length="5"/>
5030
5030
  <text>SEC16</text>
5031
5031
  </annotation>
5032
5032
  </passage>
@@ -5041,27 +5041,27 @@
5041
5041
  <text>sec13-1 sec16-2 sec23-1 sec31-1 sec17-1 sec18-1 lst1-1 - - - - + + lst2-1 - + + + ND ND lst3-1 - + + + ND ND lst4-1 +/- + + + ND ND lst5-1 - + + + ND ND lst6-1 - +/- - + - + + lst7-1 - + + + ND ND lst8-1 - + + + ND ND</text>
5042
5042
  <annotation id="Team286_A903">
5043
5043
  <infon key="type">Gene</infon>
5044
- <location offset="60918" length="76"/>
5044
+ <location offset="60923" length="76"/>
5045
5045
  <text>sec13-1 sec16-2 sec23-1 sec31-1 sec17-1 sec18-1 lst1-1 - - - - +</text>
5046
5046
  </annotation>
5047
5047
  <annotation id="Team286_A904">
5048
5048
  <infon key="type">Gene</infon>
5049
- <location offset="60999" length="22"/>
5049
+ <location offset="61004" length="22"/>
5050
5050
  <text>lst2-1 - + + + ND</text>
5051
5051
  </annotation>
5052
5052
  <annotation id="Team286_A905">
5053
5053
  <infon key="type">Gene</infon>
5054
- <location offset="61027" length="52"/>
5054
+ <location offset="61032" length="52"/>
5055
5055
  <text>lst3-1 - + + + ND ND lst4-1 +/- + + + ND</text>
5056
5056
  </annotation>
5057
5057
  <annotation id="Team286_A906">
5058
5058
  <infon key="type">Gene</infon>
5059
- <location offset="61085" length="53"/>
5059
+ <location offset="61090" length="53"/>
5060
5060
  <text>lst5-1 - + + + ND ND lst6-1 - +/- - + - +</text>
5061
5061
  </annotation>
5062
5062
  <annotation id="Team286_A907">
5063
5063
  <infon key="type">Gene</infon>
5064
- <location offset="61143" length="54"/>
5064
+ <location offset="61148" length="54"/>
5065
5065
  <text>lst7-1 - + + + ND ND lst8-1 - + + + ND ND</text>
5066
5066
  </annotation>
5067
5067
  </passage>
@@ -5101,87 +5101,87 @@
5101
5101
  </annotation>
5102
5102
  <annotation id="Team286_A912">
5103
5103
  <infon key="type">Gene</infon>
5104
- <location offset="61575" length="17"/>
5104
+ <location offset="61576" length="17"/>
5105
5105
  <text>lst1Delta mutants</text>
5106
5106
  </annotation>
5107
5107
  <annotation id="Team286_A913">
5108
5108
  <infon key="type">Gene</infon>
5109
- <location offset="61661" length="4"/>
5109
+ <location offset="61662" length="4"/>
5110
5110
  <text>LEU2</text>
5111
5111
  </annotation>
5112
5112
  <annotation id="Team286_A914">
5113
5113
  <infon key="type">Gene</infon>
5114
- <location offset="61667" length="6"/>
5114
+ <location offset="61668" length="6"/>
5115
5115
  <text>CKY534</text>
5116
5116
  </annotation>
5117
5117
  <annotation id="Team286_A915">
5118
5118
  <infon key="type">Gene</infon>
5119
- <location offset="61862" length="4"/>
5119
+ <location offset="61863" length="4"/>
5120
5120
  <text>LEU2</text>
5121
5121
  </annotation>
5122
5122
  <annotation id="Team286_A916">
5123
5123
  <infon key="type">Gene</infon>
5124
- <location offset="61919" length="6"/>
5124
+ <location offset="61920" length="6"/>
5125
5125
  <text>pRS316</text>
5126
5126
  </annotation>
5127
5127
  <annotation id="Team286_A917">
5128
5128
  <infon key="type">Gene</infon>
5129
- <location offset="61928" length="4"/>
5129
+ <location offset="61929" length="4"/>
5130
5130
  <text>LST1</text>
5131
5131
  </annotation>
5132
5132
  <annotation id="Team286_A918">
5133
5133
  <infon key="type">Gene</infon>
5134
- <location offset="61959" length="5"/>
5134
+ <location offset="61960" length="5"/>
5135
5135
  <text>pKR17</text>
5136
5136
  </annotation>
5137
5137
  <annotation id="Team286_A919">
5138
5138
  <infon key="type">Gene</infon>
5139
- <location offset="61966" length="5"/>
5139
+ <location offset="61967" length="5"/>
5140
5140
  <text>SEC24</text>
5141
5141
  </annotation>
5142
5142
  <annotation id="Team286_A920">
5143
5143
  <infon key="type">Gene</infon>
5144
- <location offset="61999" length="5"/>
5144
+ <location offset="62000" length="5"/>
5145
5145
  <text>pAF70</text>
5146
5146
  </annotation>
5147
5147
  <annotation id="Team286_A921">
5148
5148
  <infon key="type">Gene</infon>
5149
- <location offset="62009" length="5"/>
5149
+ <location offset="62010" length="5"/>
5150
5150
  <text>SEC24</text>
5151
5151
  </annotation>
5152
5152
  <annotation id="Team286_A922">
5153
5153
  <infon key="type">Gene</infon>
5154
- <location offset="62033" length="5"/>
5154
+ <location offset="62034" length="5"/>
5155
5155
  <text>pKR34</text>
5156
5156
  </annotation>
5157
5157
  <annotation id="Team286_A923">
5158
5158
  <infon key="type">Gene</infon>
5159
- <location offset="62217" length="5"/>
5159
+ <location offset="62218" length="5"/>
5160
5160
  <text>pGAL1</text>
5161
5161
  </annotation>
5162
5162
  <annotation id="Team286_A924">
5163
5163
  <infon key="type">Gene</infon>
5164
- <location offset="62223" length="4"/>
5164
+ <location offset="62224" length="4"/>
5165
5165
  <text>LST1</text>
5166
5166
  </annotation>
5167
5167
  <annotation id="Team286_A925">
5168
5168
  <infon key="type">Gene</infon>
5169
- <location offset="62229" length="5"/>
5169
+ <location offset="62230" length="5"/>
5170
5170
  <text>pKR35</text>
5171
5171
  </annotation>
5172
5172
  <annotation id="Team286_A926">
5173
5173
  <infon key="type">Gene</infon>
5174
- <location offset="62274" length="5"/>
5174
+ <location offset="62275" length="5"/>
5175
5175
  <text>pKR35</text>
5176
5176
  </annotation>
5177
5177
  <annotation id="Team286_A927">
5178
5178
  <infon key="type">Gene</infon>
5179
- <location offset="62284" length="5"/>
5179
+ <location offset="62285" length="5"/>
5180
5180
  <text>SEC24</text>
5181
5181
  </annotation>
5182
5182
  <annotation id="Team286_A928">
5183
5183
  <infon key="type">Gene</infon>
5184
- <location offset="62308" length="5"/>
5184
+ <location offset="62309" length="5"/>
5185
5185
  <text>pKR41</text>
5186
5186
  </annotation>
5187
5187
  </passage>
@@ -5201,32 +5201,32 @@
5201
5201
  </annotation>
5202
5202
  <annotation id="Team286_A931">
5203
5203
  <infon key="type">Gene</infon>
5204
- <location offset="62800" length="19"/>
5204
+ <location offset="62801" length="19"/>
5205
5205
  <text>anti-Pma1p antibody</text>
5206
5206
  </annotation>
5207
5207
  <annotation id="Team286_A932">
5208
5208
  <infon key="type">Gene</infon>
5209
- <location offset="62989" length="6"/>
5209
+ <location offset="62990" length="6"/>
5210
5210
  <text>CKY536</text>
5211
5211
  </annotation>
5212
5212
  <annotation id="Team286_A933">
5213
5213
  <infon key="type">Gene</infon>
5214
- <location offset="63100" length="18"/>
5214
+ <location offset="63101" length="18"/>
5215
5215
  <text>LST1 plasmid pKR17</text>
5216
5216
  </annotation>
5217
5217
  <annotation id="Team286_A934">
5218
5218
  <infon key="type">Gene</infon>
5219
- <location offset="63167" length="5"/>
5219
+ <location offset="63168" length="5"/>
5220
5220
  <text>SEC24</text>
5221
5221
  </annotation>
5222
5222
  <annotation id="Team286_A935">
5223
5223
  <infon key="type">Gene</infon>
5224
- <location offset="63174" length="6"/>
5224
+ <location offset="63175" length="6"/>
5225
5225
  <text>CKY536</text>
5226
5226
  </annotation>
5227
5227
  <annotation id="Team286_A936">
5228
5228
  <infon key="type">Gene</infon>
5229
- <location offset="63194" length="24"/>
5229
+ <location offset="63195" length="24"/>
5230
5230
  <text>2mu SEC24 plasmid pKR34</text>
5231
5231
  </annotation>
5232
5232
  </passage>
@@ -5246,12 +5246,12 @@
5246
5246
  </annotation>
5247
5247
  <annotation id="Team286_A939">
5248
5248
  <infon key="type">Gene</infon>
5249
- <location offset="63664" length="9"/>
5249
+ <location offset="63665" length="9"/>
5250
5250
  <text>lst1Delta</text>
5251
5251
  </annotation>
5252
5252
  <annotation id="Team286_A940">
5253
5253
  <infon key="type">Gene</infon>
5254
- <location offset="63676" length="6"/>
5254
+ <location offset="63677" length="6"/>
5255
5255
  <text>CKY536</text>
5256
5256
  </annotation>
5257
5257
  </passage>
@@ -5441,12 +5441,12 @@
5441
5441
  </annotation>
5442
5442
  <annotation id="Team286_A974">
5443
5443
  <infon key="type">Gene</infon>
5444
- <location offset="66327" length="8"/>
5444
+ <location offset="66328" length="8"/>
5445
5445
  <text>Lst1p-HA</text>
5446
5446
  </annotation>
5447
5447
  <annotation id="Team286_A975">
5448
5448
  <infon key="type">Gene</infon>
5449
- <location offset="66386" length="16"/>
5449
+ <location offset="66387" length="16"/>
5450
5450
  <text>anti-HA antibody</text>
5451
5451
  </annotation>
5452
5452
  </passage>
@@ -5466,7 +5466,7 @@
5466
5466
  </annotation>
5467
5467
  <annotation id="TEAM_336_PPI15">
5468
5468
  <infon key="type">PPImention</infon>
5469
- <location offset="66406" length="45"/>
5469
+ <location offset="66406" length="45" original_offset="0"/>
5470
5470
  <text>Two-Hybrid Interaction between LST1 and SEC23</text>
5471
5471
  </annotation>
5472
5472
  </passage>
@@ -5476,17 +5476,17 @@
5476
5476
  <text>beta-galactosidase activity LST1 SEC24 No fusion SEC23 395 +- 8 629 +- 1 24.4 +- 0.1 No fusion 30.4 +- 4.0 25.3 +- 1.4 44.8 +- 5.3</text>
5477
5477
  <annotation id="Team286_A978">
5478
5478
  <infon key="type">Gene</infon>
5479
- <location offset="66453" length="18"/>
5479
+ <location offset="66458" length="18"/>
5480
5480
  <text>beta-galactosidase</text>
5481
5481
  </annotation>
5482
5482
  <annotation id="Team286_A979">
5483
5483
  <infon key="type">Gene</infon>
5484
- <location offset="66482" length="4"/>
5484
+ <location offset="66487" length="4"/>
5485
5485
  <text>LST1</text>
5486
5486
  </annotation>
5487
5487
  <annotation id="Team286_A980">
5488
5488
  <infon key="type">Gene</infon>
5489
- <location offset="66488" length="5"/>
5489
+ <location offset="66493" length="5"/>
5490
5490
  <text>SEC24</text>
5491
5491
  </annotation>
5492
5492
  </passage>
@@ -5601,7 +5601,7 @@
5601
5601
  </annotation>
5602
5602
  <annotation id="TEAM_336_PPI16">
5603
5603
  <infon key="type">PPImention</infon>
5604
- <location offset="66859" length="97"/>
5604
+ <location offset="66859" length="97" original_offset="0"/>
5605
5605
  <text>Lst1p/-Sec23p complex is membrane associated. (A) Affinity isolation of Lst1p/-Sec23p complexes.</text>
5606
5606
  </annotation>
5607
5607
  </passage>
@@ -5883,7 +5883,7 @@
5883
5883
  <text>Yeast SEC16gene encodes a multidomain vesicle coat protein that interacts with Sec23p</text>
5884
5884
  <annotation id="TEAM_336_PPI17">
5885
5885
  <infon key="type">PPImention</infon>
5886
- <location offset="69406" length="85"/>
5886
+ <location offset="69406" length="85" original_offset="0"/>
5887
5887
  <text>Yeast SEC16gene encodes a multidomain vesicle coat protein that interacts with Sec23p</text>
5888
5888
  </annotation>
5889
5889
  </passage>
@@ -5907,7 +5907,7 @@
5907
5907
  <text>SED4encodes a yeast endoplasmic reticulum protein that binds Sec16p and participates in vesicle formation</text>
5908
5908
  <annotation id="TEAM_336_PPI18">
5909
5909
  <infon key="type">PPImention</infon>
5910
- <location offset="69493" length="105"/>
5910
+ <location offset="69493" length="105" original_offset="0"/>
5911
5911
  <text>SED4encodes a yeast endoplasmic reticulum protein that binds Sec16p and participates in vesicle formation</text>
5912
5912
  </annotation>
5913
5913
  </passage>
@@ -5925,7 +5925,7 @@
5925
5925
  <text>COPII coat subunit interactions: Sec24p and Sec23p bind to adjacent regions of Sec16p</text>
5926
5926
  <annotation id="TEAM_336_PPI19">
5927
5927
  <infon key="type">PPImention</infon>
5928
- <location offset="69599" length="85"/>
5928
+ <location offset="69599" length="85" original_offset="0"/>
5929
5929
  <text>COPII coat subunit interactions: Sec24p and Sec23p bind to adjacent regions of Sec16p</text>
5930
5930
  </annotation>
5931
5931
  </passage>
@@ -5958,7 +5958,7 @@
5958
5958
  <text>Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2</text>
5959
5959
  <annotation id="TEAM_336_PPI20">
5960
5960
  <infon key="type">PPImention</infon>
5961
- <location offset="69735" length="74"/>
5961
+ <location offset="69735" length="74" original_offset="0"/>
5962
5962
  <text>Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2</text>
5963
5963
  </annotation>
5964
5964
  </passage>