roebe 0.5.117
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- checksums.yaml +7 -0
- data/README.md +2516 -0
- data/bin/blinking_cursor +7 -0
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- data/bin/fragment_maker +7 -0
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- data/bin/hello_world +7 -0
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- data/bin/rfirefox +7 -0
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- data/bin/roebeshell +11 -0
- data/bin/ruby_cat +7 -0
- data/bin/ruby_dhcpcd +7 -0
- data/bin/run +7 -0
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- data/bin/show_ten_aliases +7 -0
- data/bin/simple_extractor +9 -0
- data/bin/symlink_directories_from_that_directory_to_the_current_directory +7 -0
- data/bin/symlink_everything_from_that_directory_to_this_directory +7 -0
- data/bin/symlink_files_from_that_directory_to_the_current_directory +7 -0
- data/bin/take_screenshot +7 -0
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- data/bin/trad +7 -0
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- data/doc/sinatra_tutorial/sinatra_tutorial.cgi +7 -0
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- data/doc/todo/todo_for_the_roebe_shell.md +1192 -0
- data/lib/roebe/autoinclude.rb +4 -0
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- data/lib/roebe/browser/README.md +5 -0
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- data/lib/roebe/cat/cat.rb +137 -0
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p.default {
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margin: 4px;
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margin-left: 1em;
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margin-right: 1em;
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padding: 5px;
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}
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'+TEMPLATE1
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ruby_favicon
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body_css_class 's12px padt4px marpad2px VERDANAs'
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body_css_style 'background-color: #d3d2d1;'
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font_size 'def'
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# =========================================================================== #
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# === header
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# =========================================================================== #
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def header(
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i = '',
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optional_css_class = ''
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)
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h2 i, optional_css_class
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end
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doc {
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dimg2 'science/genetics/DNA_HELIX_UND_RATTE.png',
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'https://i.imgur.com/tHpLvvL.jpg',
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'bblack3 mars0_5em'
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br
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# ========================================================================= #
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# === Tabelle der Nukleoside
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# ========================================================================= #
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h2 'Tabelle der Nukleoside'
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br
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table2('mars2em larger BOLD','','',
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'Basen:','Nukleoside:',
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'Adenin ←','→ Adenosin',
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'Guanin ←','→ Guanosin',
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'Thymin ←','→ Thymidin',
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'Cytosin ←','→ Cytidin',
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'Uracil ←','→ Uridin'
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)
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pretty_hsep
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header 'Sigma-Faktoren','mart1px marb10px'
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p_default {
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e 'Sigma 32 bei E.coli erkennt eine andere Sequenz als (Standard)
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Sigma 70. Ich tausche einen Teil der Polymerase aus - und schon
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funktioniert sie komplett anders!'
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}
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pretty_hsep
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# ========================================================================= #
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# === Nomenklatur von Genen
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# ========================================================================= #
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header 'Nomenklatur der Gene'
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div('default martb1px') {
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p('marb1px') {
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e 'Die verschiedenen <b>Namen</b> für Gene entstammen oft der
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Geschichte ihrer Entdeckung - sprich, wann, wie und von welcher
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Person oder von welchen Personen sie entdeckt wurden:'
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br
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lem2 '- Ein Zellbiologie gibt den Namen anhand der zellulären Funktion.'
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br
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lem2 '- Ein Entwicklungsbiologe anhand von Mutanten die defekt sind.'
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}
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}
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# ========================================================================= #
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# === ESTs
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# ========================================================================= #
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h2 'ESTs'
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div {
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p {
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e '<b>ESTs</b> (aka <b>expressed sequence tags</b> are short sequences
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+
derived from messenger RNAs. These can, in turn, be hybridized to
|
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+
chromosomal DNA. The advantage of this was that this allowed
|
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+
scientists in the 1990s to easily identify open reading frames.'
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}
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}
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# ========================================================================= #
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# === Historische Fakten tag
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# ========================================================================= #
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h2 'Historische und kuriose Fakten'
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div_default {
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p_default {
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e 'Im Jahre <b>1970</b> sequenzierte <b>Ray Wu</b> als erster ein DNA
|
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Fragment - zwölf Basen am Ende eines Einzelstrangs entstanden aus
|
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+
einem durch Enzyme geschnittenen Ring.'
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+
}
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+
}
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+
# ========================================================================= #
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# === DNA Microarrays tag
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+
# ========================================================================= #
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header 'DNA Microarrays','mart1px'
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div('default ind0 wid95 mart2px','dna_microarrays'){
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e '<b>DNA Microarrays</b> make it possible to create a global profile
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+
of all expressed RNA within a cell or cell homogenisate.'
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br
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e 'This pattern that will be capture is often very characteristic
|
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+
of the given cell or tissue, which in turn allows characterization
|
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+
of disease patterns (in theory).'
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+
br
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+
dimg 'science/DNA_MICROARRAY.png',
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+
'mars3em'
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+
}
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+
# ========================================================================= #
|
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+
# === MITOCHONDRIUM
|
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# ========================================================================= #
|
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div(id: 'mitochondrium'){
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h4 'Mitochondrium'
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dimg 'science/biology/organelles/MITOCHONDRIUM_OVERVIEW.png',
|
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'bblack3 marl1em'
|
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+
dimg 'science/biology/organelles/TEM_Bild_eines_Mitochondriums.png',
|
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+
'bblack3'
|
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+
}
|
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+
# ========================================================================= #
|
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+
# === Thymidylat-Synthase Tag. dUMP -> dTMP
|
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+
# ========================================================================= #
|
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div {
|
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+
h2 'Thymidylat-Synthase'
|
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p {
|
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e 'dUMP wird durch <b>Thymidylat-Synthase</b> in dTMP umgewandelt,
|
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+
indem eine Methylgruppe angeheftet wird.'
|
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+
}
|
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+
}
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+
# =========================================================================== #
|
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+
# === misc tag tidbits tag
|
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+
# =========================================================================== #
|
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|
+
div {
|
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e 'Helikasen wurden im Jahre 1976 durch <b>Hoffman-Berling</b> entdeckt.'
|
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+
br
|
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+
e 'SSB ist auch insofern wichtig, da es <b>secondary structures</b>
|
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+
reduziert.'
|
139
|
+
br
|
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|
+
e '<b>RecA</b> wird durch ATP "angeregt", die Affinität zu DNA
|
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|
+
steigt.'
|
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|
+
br
|
143
|
+
e 'Die <b>Shine-Dalgarno Sequenz</b> auf der mRNA ist AGG-AGG-U'
|
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|
+
br
|
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|
+
e 'Wo natürlicherweise Kalkstein auftritt, ist der Boden weniger sauer.'
|
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|
+
}
|
147
|
+
# ========================================================================= #
|
148
|
+
# === Gene Map Viewer
|
149
|
+
# ========================================================================= #
|
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|
+
header 'Gene Map Viewers (Ensembl and others)'
|
151
|
+
div('ind0 wid95 mart2px'){
|
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+
e 'The Ensemble Gene Map Viewer contains entries for humans,
|
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+
chicken, fugu, fruitfly and many other organisms.'
|
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+
br
|
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+
abr 'https://www.ensembl.org/index.html',
|
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+
content: 'Ensembl Gene Map Viewer - Show different Animals '\
|
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|
+
'and Human Genomes',
|
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|
+
css_class: 'BOLD mars1em'
|
159
|
+
br
|
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|
+
e 'The above Ensembl Application has been written in perl.'
|
161
|
+
br
|
162
|
+
e 'Especially these reviews may be helpful for anyone curious
|
163
|
+
to find out more about it:'
|
164
|
+
br
|
165
|
+
e 'James Stalker et al. The Ensembl Web Site: Mechanics of
|
166
|
+
a Genome Browser Genome Res. 2004 14: 951-955.'
|
167
|
+
br
|
168
|
+
e 'It can be found here:'
|
169
|
+
br
|
170
|
+
abr 'https://www.genome.org/cgi/content/abstract/14/5/951',
|
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|
+
content: 'Review of the GeneMap Viewer',
|
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|
+
css_class: 'BOLD mars1em'
|
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|
+
abr 'https://www.ensembl.org/Docs/',
|
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|
+
content: 'Good Documents for Ensembl',
|
175
|
+
css_class: 'BOLD mars1em'
|
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|
+
}
|
177
|
+
# ========================================================================= #
|
178
|
+
# === Die Terminale Transferase
|
179
|
+
# ========================================================================= #
|
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|
+
header 'Die Terminale Transferase'
|
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|
+
div {
|
182
|
+
lembre '<b>Terminale Tranferase</b> katalysiert die Addition von
|
183
|
+
Nukleotiden an das <b>3-Strich Ende von DNA</b>.'
|
184
|
+
lembre "Interessanterweise arbeitet dieses Enzym an ssDNA und benötigt
|
185
|
+
keinen Primer. Es scheint auch Ribonucleotide an die 3' Enden von DNA
|
186
|
+
hinzufügen zu können."
|
187
|
+
lembre 'Cobalt ist ein Kofaktor dieses Enzyms.'
|
188
|
+
}
|
189
|
+
# ========================================================================= #
|
190
|
+
# === Cosuppression tag (ist NICHT Codominance!)
|
191
|
+
# ========================================================================= #
|
192
|
+
header 'Cosuppression'
|
193
|
+
div {
|
194
|
+
e '<b>Cosuppression</b> ist dann zu beobachten wenn sowohl das
|
195
|
+
<b>Transgen</b>, als auch das <b>homologe endogeneous Gene
|
196
|
+
gesilenced</b> werden.'
|
197
|
+
br
|
198
|
+
e 'Der Effekt wird durch <b>dsRNA</b> hervorgerufen.'
|
199
|
+
br
|
200
|
+
e 'Es wurde zuerst in Pflanzen, dann in Pilzen und auch in Tieren
|
201
|
+
beobachtet - vor allem die Cosuppression in Petunias wurde bekannt:'
|
202
|
+
br
|
203
|
+
dimg 'science/biology/PLANTS/COSUPPRESSION_IN_PETUNIAS.gif',
|
204
|
+
'bblack3 marl2em'
|
205
|
+
br
|
206
|
+
e 'Picture above: A variegated petunia. Upon injection of the
|
207
|
+
gene responsible for purple colouring in petunias, the flowers
|
208
|
+
became variegated or white rather than deeper purple as was
|
209
|
+
expected.','italic marl2em smaller'
|
210
|
+
br
|
211
|
+
e 'Cosuppression führt effektiv zur <b>Degradierung von RNA</b>.'
|
212
|
+
br
|
213
|
+
e 'Dies kann man dann auch "grafting-induced" Cosuppression nennen,
|
214
|
+
vor allem in Pflanzen.'
|
215
|
+
br
|
216
|
+
ee 'Man vermutet das dieser Mechanismus in eukaryotischen Organismen
|
217
|
+
dadurch entstand um molekulare Parasiten wie Viren oder Transpososon
|
218
|
+
zu "bekämpfen" (Kasschau, K.D. & Carrington, J.C. Cell 95, 461-470 (1998). '
|
219
|
+
abr_self 'https://www.ncbi.nlm.nih.gov/pubmed/9827799?dopt=Abstract&holding=npg)'
|
220
|
+
br
|
221
|
+
e 'Diese Vermutung wird unterstützt von der Tatsache das einige
|
222
|
+
Pflanzen RNA-Viren <b>Cosuppression inhibieren</b> können.'
|
223
|
+
br
|
224
|
+
abr 'https://www.nature.com/ng/journal/v21/n2/full/ng0299_148.html',
|
225
|
+
content: 'Cosuppression'
|
226
|
+
}
|
227
|
+
# ========================================================================= #
|
228
|
+
# === IMG TAG
|
229
|
+
# ========================================================================= #
|
230
|
+
div(id: 'codon') {
|
231
|
+
dimg 'science/biology/RNA_BASE_CODE.png',
|
232
|
+
'bblack3'
|
233
|
+
}
|
234
|
+
# ========================================================================= #
|
235
|
+
# === Methylcytosin und Methyl.
|
236
|
+
# ========================================================================= #
|
237
|
+
div('mart1em','methylcytosin') {
|
238
|
+
p {
|
239
|
+
e 'In eukaryotischer DNA liegen methylierte Cytosine immer neben
|
240
|
+
Guaninen desselben Stranges. (<b>CpG</b> Inseln, wobei p für
|
241
|
+
<b>Phosphodiesterbrücke</b> steht)'
|
242
|
+
br
|
243
|
+
e 'Häufig kann man in Bakterien methylierte Adenin-Rest finden -
|
244
|
+
<b>6-Methylaminopurin</b>.'
|
245
|
+
}
|
246
|
+
}
|
247
|
+
# ========================================================================= #
|
248
|
+
# === Meiose tag
|
249
|
+
# ========================================================================= #
|
250
|
+
header 'Meiose (griechisch: <b>meiono</b> = vermindern)'
|
251
|
+
div {
|
252
|
+
p {
|
253
|
+
e 'Die <b>Prophase I</b> der Meiose ist unterteilt in: (LeZyPaDip-Dia)'
|
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|
+
br
|
255
|
+
cmd ' Leptotän'
|
256
|
+
cmd ' Zygotän'
|
257
|
+
cmd ' Pachytän'
|
258
|
+
cmd ' Diplotän'
|
259
|
+
cmd ' Diakinese'
|
260
|
+
}
|
261
|
+
}
|
262
|
+
# ========================================================================= #
|
263
|
+
# === F-Plasmid PHAGE
|
264
|
+
# ========================================================================= #
|
265
|
+
div(id: 'f_plasmid'){
|
266
|
+
h4 'F Plasmid'
|
267
|
+
p {
|
268
|
+
e 'Das F Plasmid insertiert mittels IS Sequenzen.'
|
269
|
+
}
|
270
|
+
}
|
271
|
+
# ========================================================================= #
|
272
|
+
# === CCA adding Enzyme
|
273
|
+
# ========================================================================= #
|
274
|
+
header 'CCA adding Enzyme'
|
275
|
+
div {
|
276
|
+
p('default') {
|
277
|
+
lem 'An dieser Stelle werden einige Fakten zum <b>CCA-adding Enzyme</b>
|
278
|
+
erwähnt.'
|
279
|
+
br
|
280
|
+
lem "Dieses Enzym hängt an 3' Ende von tRNAs die Sequenz <b>CCA</b> an."
|
281
|
+
br
|
282
|
+
lem 'Interessanterweise will dieses Enzym lieber CCC hinzufügen,
|
283
|
+
die Anwesenheit von ATP verhindert dies jedoch, und so wird
|
284
|
+
als letzter Schritt ein Adenin hinzugefügt.'
|
285
|
+
br
|
286
|
+
lem 'ATP scheint auch das weitere Hinzufügen von Cytosin zu verhindern.'
|
287
|
+
br
|
288
|
+
lem 'Mit anderen Worten - E. coli CCA enzyme ist eigentlich eine
|
289
|
+
poly(C) Polymerase, aber dank der Gegenwart von ATP
|
290
|
+
wird die Spezifität geändert.'
|
291
|
+
br
|
292
|
+
lem 'Dies erinnert an die poly(A) polymerase.'
|
293
|
+
}
|
294
|
+
}
|
295
|
+
# ========================================================================= #
|
296
|
+
# Chemische Struktur von DNA
|
297
|
+
# ========================================================================= #
|
298
|
+
header 'Chemische Struktur der DNA'
|
299
|
+
div {
|
300
|
+
dimg :chemische_struktur_dna,
|
301
|
+
'bblack1 marl1em'
|
302
|
+
}
|
303
|
+
# ========================================================================= #
|
304
|
+
# === Wobble Regeln in der Genetik
|
305
|
+
# ========================================================================= #
|
306
|
+
div {
|
307
|
+
h2 'Wobble Regeln in der Genetik',
|
308
|
+
'marb2px',
|
309
|
+
'wobble' # rf stdk wobble
|
310
|
+
table2('marl2em pad0_5em','','RANDOM_BORDER_2PX',
|
311
|
+
"<b>5' end of Anticodon (tRNA)</b>","<b>3' end of Codon (mRNA)</b>",
|
312
|
+
'G','C/U',
|
313
|
+
'A','U',
|
314
|
+
'C','G',
|
315
|
+
'U','A/G',
|
316
|
+
'I','U/C/A'
|
317
|
+
)
|
318
|
+
}
|
319
|
+
# ========================================================================= #
|
320
|
+
# === Phenylthiocarbamide tag
|
321
|
+
# ========================================================================= #
|
322
|
+
div {
|
323
|
+
h2 'PTC - Phenylthiocarbamide'
|
324
|
+
p {
|
325
|
+
dimg 'science/chemistry/PHENYLTHIOCARBAMIDE.png'
|
326
|
+
br
|
327
|
+
e '<b>Phenylthiocarbamide</b> ist eine organische Verbindung, die
|
328
|
+
entweder sehr bitter schmeckt, oder überhaupt nicht - je nachdem
|
329
|
+
welche Gene der Träger hat. (Die Fähigkeit PTC zu schmecken
|
330
|
+
ist ein dominante Eigenschaft des Gens, und der Test auf
|
331
|
+
<b>PTC Sensitivity</b> ist einer der häufigsten genetischen
|
332
|
+
Tests bei Menschen.)'
|
333
|
+
}
|
334
|
+
}
|
335
|
+
# ========================================================================= #
|
336
|
+
# === Hyperchromizität
|
337
|
+
# ========================================================================= #
|
338
|
+
h4 'Hyperchromizität'
|
339
|
+
p {
|
340
|
+
e '<b>Einzelsträngige DNA</b> absorbiert Ultraviolettstrahlung anders
|
341
|
+
als doppelsträngige DNA. Bei einer Wellenlänge von <b>260nm</b>
|
342
|
+
absorbiert ssDNA diese Strahlung 1,4 mal mehr als dsDNA - dies
|
343
|
+
ist mit dem Begriff <b>Hyperchromizität</b> gemeint.'
|
344
|
+
}
|
345
|
+
# ========================================================================= #
|
346
|
+
# === andi tag
|
347
|
+
# ========================================================================= #
|
348
|
+
div('default') {
|
349
|
+
h2 'ANDi','','andi'
|
350
|
+
dimg 'science/biology/ANIMALS/ANDi.jpg',
|
351
|
+
'bblack3'
|
352
|
+
e 'Der Affe ANDi - reverser Name, für <i class="BOLD ud">integrated
|
353
|
+
DNA</i> wurde 2000 gezeugt.'
|
354
|
+
p {
|
355
|
+
e 'Im <b>Januar 2000</b> erblickte ANDi in einem Labor der <b>Oregon
|
356
|
+
Health Science University</b> das Licht der Welt: Als erster
|
357
|
+
transgener Rhesusaffe, der Erbinformationen einer anderen Art
|
358
|
+
in seinen Zellen trägt.'
|
359
|
+
br
|
360
|
+
e 'Das aufwändige Experiment mit hoher Fehlerquote löste
|
361
|
+
kontroverse Diskussionen aus.'
|
362
|
+
}
|
363
|
+
p {
|
364
|
+
e 'Leicht war der Weg zu diesem Erfolg nicht: <b>224 Eizellen</b> von
|
365
|
+
Rhesus-Affen wurden mit dem Quallen-Gen manipuliert. Nach der künstlichen
|
366
|
+
Befruchtung dieser Eizellen entstanden 40 Embryos. Die wurden wiederum in
|
367
|
+
20 Leihmütter eingepflanzt, von denen nur fünf schwanger wurden. Nur
|
368
|
+
drei Affenbabys kamen am Ende lebend zur Welt - ANDi und seine
|
369
|
+
Spielkameraden. Doch nur ANDi trägt das Leuchtgen in seinem Erbgut.'
|
370
|
+
}
|
371
|
+
p {
|
372
|
+
e '<b>Wie</b> wurde <b>ANDi</b> manipuliert?'
|
373
|
+
br
|
374
|
+
e 'Die Forscher injizieren <b>nicht-infektiöse Viren</b> in eine unbefruchtete
|
375
|
+
Eizelle. Die DNA des Virus ist gleichzeitig Träger des Leuchtgens. Sie
|
376
|
+
dringt in die Chromosomen der unbefruchteten Eizelle ein. Dort baut sich
|
377
|
+
das fremde Gen in die DNA des Empfängers ein. Das Erbgut der Eizelle
|
378
|
+
trägt jetzt das Gen einer Qualle.'
|
379
|
+
}
|
380
|
+
p {
|
381
|
+
e 'Im nächsten Schritt wird das Ei künstlich befruchtet und der Embryo
|
382
|
+
einer Leihmutter eingepflanzt. Nach fünfeinhalb Monaten Schwangerschaft
|
383
|
+
wird Andi geboren. Unter dem Mikroskop wird sichtbar, dass das
|
384
|
+
Experiment geglückt ist:'
|
385
|
+
br
|
386
|
+
e 'Seine Körperzellen leuchten in fluoreszierendem Grün.','marl1em'
|
387
|
+
br
|
388
|
+
e 'Das Problem das Gerald Schatten von der Oregon Health University
|
389
|
+
hatte war das es keine isolierten Affen ES Zellen gab.'
|
390
|
+
br
|
391
|
+
e 'Als Alternative musste man einen Retrovirus verwenden, um
|
392
|
+
eine Integration der viralen DNA in die DNA der Zelle zu
|
393
|
+
ermöglichen.'
|
394
|
+
br
|
395
|
+
e 'Die Form des Retrovirus war ein "pseudo-typed retrovirus" -
|
396
|
+
es kann noch ins Genom integrieren, kann aber keine Krankheit
|
397
|
+
mehr auslösen da die meisten anderen Gene entfernt wurden.'
|
398
|
+
}
|
399
|
+
p {
|
400
|
+
e 'By placing a foreign gene (transgene) into a pseudo-typed
|
401
|
+
retrovirus and injecting the virus into the ova of an
|
402
|
+
organism, the foreign gene is integrated into the eggs’
|
403
|
+
DNA.'
|
404
|
+
br
|
405
|
+
e 'In vitro fertilization can then be used to produce embryos. Every cell in the resulting
|
406
|
+
embryo carries the foreign gene because every cell comes from
|
407
|
+
the original fertilized ovum.'
|
408
|
+
br
|
409
|
+
e 'This is the strategy used by the scientists to insert the GFP gene
|
410
|
+
into a rhesus monkey.'
|
411
|
+
br
|
412
|
+
e 'The problem with this strategy is that there is no control over the site
|
413
|
+
of integration of the transgene.'
|
414
|
+
e 'Integration into the genome is random, and this can have unwanted side-effects.'
|
415
|
+
e 'If the transgene inserts into another gene, the functioning of that gene can
|
416
|
+
be altered or completely eliminated which may result in developmental defects.'
|
417
|
+
}
|
418
|
+
p {
|
419
|
+
e 'Ein Hauptproblem dabei ist, das die Integration ins Genom zufällig erfolgen
|
420
|
+
kann - und an manchen Stellen im Genom wird diese Information überhaupt
|
421
|
+
nicht mehr aufgerufen, und damit auch nicht mehr produziert.'
|
422
|
+
}
|
423
|
+
p {
|
424
|
+
e ' A. W. S. Chan, K. Y. Chong, C. Martinovich, C. Simerly, G. Schatten*,
|
425
|
+
"Transgenic Monkeys Produced by Retroviral Gene Transfer into Mature Oocytes"
|
426
|
+
Science, Vol. 291, pp. 309-312 (January 12, 2001).'
|
427
|
+
br
|
428
|
+
a 'https://www.sciencemag.org/cgi/content/abstract/291/5502/309',
|
429
|
+
content: 'Abstract',
|
430
|
+
css_class: 'BOLD mars1em'
|
431
|
+
}
|
432
|
+
}
|
433
|
+
# ========================================================================= #
|
434
|
+
# === pathways tag
|
435
|
+
# ========================================================================= #
|
436
|
+
h2 'Genetic and Biochemical Pathways'
|
437
|
+
div('default') {
|
438
|
+
p {
|
439
|
+
e 'Neurospora Merksatz:'
|
440
|
+
br
|
441
|
+
e 'Ornithin-Citrullin-Arginin (<b>OCA</b>)',
|
442
|
+
'marl2em'
|
443
|
+
}
|
444
|
+
}
|
445
|
+
# ======================================================================= #
|
446
|
+
# === Das Werner Syndrom
|
447
|
+
# ======================================================================= #
|
448
|
+
header 'Werner Syndrom (WS)'
|
449
|
+
div {
|
450
|
+
e 'Werner Syndrome (WS) is a rare progeroid disorder that gives
|
451
|
+
rise to <b class="ud">rapid aging phenotype</b> and increased cancer
|
452
|
+
predisposition.'
|
453
|
+
br
|
454
|
+
e 'WS is caused by mutations to a single gene, WRN, that was
|
455
|
+
identified in 1997.'
|
456
|
+
br
|
457
|
+
e 'WRN encodes for a <b>1432 amino acid protein</b>, WRN, that is
|
458
|
+
known to contain both exonuclease and helicase activities.'
|
459
|
+
br
|
460
|
+
e 'WRN belongs to the <b>RecQ helicase</b> family of genes, the
|
461
|
+
first member of which was discovered in E. coli. The family is
|
462
|
+
quite ubiquitously spread throughout the three domains of life.'
|
463
|
+
br
|
464
|
+
e 'A RecQ helicase is present as a single copy in most unicellular
|
465
|
+
organisms and multiple copies in higher eukaryotes. Five RecQ
|
466
|
+
helicases are present in humans, three of which give rise to
|
467
|
+
genetic defects, WS, Bloom syndrome (BS) and Rothmund-Thomson
|
468
|
+
syndrome (RTS).'
|
469
|
+
br
|
470
|
+
e 'However, WRN is unique among RecQ helicases
|
471
|
+
in coupling an exonuclease domain coupled to a helicase
|
472
|
+
domain, on the same polypeptide.'
|
473
|
+
br
|
474
|
+
dimg 'science/WERNER_SYNDROME.jpg',
|
475
|
+
'marl1em bblack1'
|
476
|
+
}
|
477
|
+
# ========================================================================= #
|
478
|
+
# === Pedigree symbols explained
|
479
|
+
# ========================================================================= #
|
480
|
+
h2 'Pedigree symbols explained'
|
481
|
+
div('default') {
|
482
|
+
dimg 'science/genetics/pedigree_symbols_explained.png',
|
483
|
+
'bblack1 mars3em'
|
484
|
+
}
|
485
|
+
# ========================================================================= #
|
486
|
+
# === Slogans
|
487
|
+
# ========================================================================= #
|
488
|
+
h2 'Slogans'
|
489
|
+
p_default {
|
490
|
+
e '- The minor DNA groove is less rich in chemical information
|
491
|
+
than is the major DNA groove.'
|
492
|
+
br
|
493
|
+
e '- Each gene discovery project begins with a hunt for mutants
|
494
|
+
affecting the biological process that is the focus of the
|
495
|
+
research.'
|
496
|
+
br
|
497
|
+
e '- In Bakterien kodiert <b>rpoH</b> für den Sigmafaktor 32.'
|
498
|
+
br
|
499
|
+
e '- Das Trockengewicht einer typischen Prokaryotenzelle
|
500
|
+
besteht zu rund 50% aus Kohlenstoff.'
|
501
|
+
br
|
502
|
+
e '- In Textilien wird oft <b class="FI">Lindan</b> verwendet,
|
503
|
+
ein Giftstoff.'
|
504
|
+
br
|
505
|
+
e '- <b>Aldosteron</b> scheint mitzuhelfen, das man im Alter besser hört.'
|
506
|
+
br
|
507
|
+
e '- DNA is the ultimate molecule.'
|
508
|
+
br
|
509
|
+
e '- Epigenetic silencing can be inherited from one cell
|
510
|
+
generation to the next.'
|
511
|
+
br
|
512
|
+
e '- Can an organism be conceived of as a mere mosaic of
|
513
|
+
traits determined by genes?'
|
514
|
+
br
|
515
|
+
e '- The genome is the living blueprint of an organism.'
|
516
|
+
br
|
517
|
+
e '- At around the time when the word genes was defined,
|
518
|
+
genes could be regarded as abstract elements in an
|
519
|
+
equally abstract space.'
|
520
|
+
br
|
521
|
+
e '- Conjugation is sex-like fusion.'
|
522
|
+
br
|
523
|
+
e '- RNA ist reaktiver als DNA, da der Zucker eine zusätzliche
|
524
|
+
OH-Gruppe am 2-prime C-Atom enthält.'
|
525
|
+
br
|
526
|
+
e '- One common grouping of transposable elements is whether
|
527
|
+
they have to make use of reverse transcription in order to
|
528
|
+
transpose or whether they do not.'
|
529
|
+
br
|
530
|
+
e 'Die verfügbare Anzahl an tRNAs in einer Zelle mag sich
|
531
|
+
auf die Geschwindigkeit der Translation auswirken. Wenn bestimmte
|
532
|
+
tRNAs nur selten vorkommen, dann wird das Ribosom bei
|
533
|
+
der Translation nicht zügig voranschreiten können.'
|
534
|
+
br
|
535
|
+
e '- As early as the 1920s, many geneticists came to believe
|
536
|
+
that genes must be material particles.'
|
537
|
+
br
|
538
|
+
e '- Autocatalysis in genetics refers to the capacity of genes
|
539
|
+
to initiate synthetic processes that ultimately yield copies
|
540
|
+
of the genes themselves in order to propagate the genetic
|
541
|
+
material from cell to cell and from generation to generation.'
|
542
|
+
br
|
543
|
+
e '- The F-plasmid can assume both an autonomous and an
|
544
|
+
integrated state.'
|
545
|
+
br
|
546
|
+
e '- CpG (CG) in eukaryotic DNA acts as an <b>epigenetic
|
547
|
+
signal</b>.'
|
548
|
+
br
|
549
|
+
e '- Genetically identical twings might still be epigenetically different.'
|
550
|
+
br
|
551
|
+
e '- Centromeres are epigenetically defined.'
|
552
|
+
br
|
553
|
+
e '- Chromatin structure is able to regulate the expression of genes.'
|
554
|
+
br
|
555
|
+
e '- Classical genetics was a method to expand biological knowledge.'
|
556
|
+
br
|
557
|
+
e '- Geneticists make a large proportion of their inferences by
|
558
|
+
manipulating the system and observing the effects.'
|
559
|
+
br
|
560
|
+
e '- Maternal imprinting means that a copy of a gene derived from
|
561
|
+
the mother will become inactivated.'
|
562
|
+
br
|
563
|
+
e '- The highly efficient system of homologous recombination in
|
564
|
+
Yeast allows the alteration of any chosen chromosomal
|
565
|
+
sequence at will.'
|
566
|
+
br
|
567
|
+
e '- AUGA ist eine besondere Sequenz, da sie sowohl ein Startcodon
|
568
|
+
(AUG) als auch ein Stopcodon (UGA) besitzt.'
|
569
|
+
br
|
570
|
+
e '- The hunt for the molecular basis of heredity began in earnest
|
571
|
+
with an experiment by Frederick Griffith, who was wirking with
|
572
|
+
a virulent strain of Streptococcus pneumoniae.'
|
573
|
+
br
|
574
|
+
e '- The Avery–MacLeod–McCarty experiment in 1944 showed that
|
575
|
+
the "transforming principle" is in fact DNA.'
|
576
|
+
br
|
577
|
+
e '- Crossovers können sich gegenseitig inhibieren, das heisst
|
578
|
+
das in unmittelbarer Nähe eines Crossovers kein weiteres
|
579
|
+
auftritt.'
|
580
|
+
br
|
581
|
+
e '- All cells are unified by the facts that their genetic
|
582
|
+
blueprints are encoded in DNA.'
|
583
|
+
br
|
584
|
+
e "- Alfred Sturtevant's experiments showed that the phenotypic
|
585
|
+
expression of a mutation could be affected by the location of
|
586
|
+
the mutated gene on the chromosome."
|
587
|
+
br
|
588
|
+
e '- Chromatin loops are a higher level of chromatin structure.'
|
589
|
+
br
|
590
|
+
e '- RNA folds up by intramolecular base pairing.'
|
591
|
+
br
|
592
|
+
e '- Die Aufgabe von mTORC1 ist es die Translation von
|
593
|
+
Proteinen zu aktivieren.'
|
594
|
+
br
|
595
|
+
e '- Imprinted genes can often be found to be clustered together,
|
596
|
+
aka aggregated as a unit.'
|
597
|
+
br
|
598
|
+
e '- Plasmids are often important for virulence; for
|
599
|
+
instance, <i>Bacillus</i> anthracis has 2 plasmids required
|
600
|
+
for is virulence properties.'
|
601
|
+
br
|
602
|
+
e '- Die Modifikation von Histonen verändert die
|
603
|
+
Chromatinfunktion.'
|
604
|
+
br
|
605
|
+
e '- The diploid character of the eukaryotic cell allows for
|
606
|
+
a mutation in one gene to be masked by a second, unmutated
|
607
|
+
gene copy.'
|
608
|
+
br
|
609
|
+
e '- Die Telomerase regeneriert die Telomere.'
|
610
|
+
br
|
611
|
+
e '- Die SRY-Region kann sich "bewegen", zum Beispiel auf
|
612
|
+
das X-Chromosom.'
|
613
|
+
br
|
614
|
+
e '- Heterochromatin propagates from a nucleation event.'
|
615
|
+
br
|
616
|
+
e '- Perizentrische Inversionen kann man oft daran
|
617
|
+
erkennen, das das Centromer seine Position im
|
618
|
+
Chromosom ändert.'
|
619
|
+
br
|
620
|
+
e '- Die Funktion des Zellkerns ist die Aufbewahrung
|
621
|
+
der DNA.'
|
622
|
+
br
|
623
|
+
e '- HP-1, heterochromatin protein-1, is required in
|
624
|
+
some way to generate the higher order chromatin
|
625
|
+
structure associated with heterochromatin.'
|
626
|
+
br
|
627
|
+
e '- DNA Methylation acts as an epigenetic mark.'
|
628
|
+
br
|
629
|
+
e '- Der Unterschied zwischen dem Menschen- und Schimpansengenom
|
630
|
+
liegt hauptsächlich in der chromosomalen Organisation (und
|
631
|
+
nicht in der DNA Sequenz)'
|
632
|
+
br
|
633
|
+
e '- Der Spindel-Checkpoint arretiert die Teilung bis alle
|
634
|
+
Chromosomen richtig in der Spindel angeordnet sind.'
|
635
|
+
br
|
636
|
+
e '- Transposons create mutations and can thusly be considered to
|
637
|
+
be one of the raw materials for evolution.'
|
638
|
+
br
|
639
|
+
e '- The dark-staining bands in polytene chromosomes act as
|
640
|
+
useful chromosomal landmarks.'
|
641
|
+
br
|
642
|
+
e '- In most diploid eukaryotes, sexual reproduction is the
|
643
|
+
only natural mechanism for producing new members of the
|
644
|
+
species.'
|
645
|
+
br
|
646
|
+
e '- The total gene content of all organisms inhabiting an
|
647
|
+
environment is known as its metagenome.'
|
648
|
+
br
|
649
|
+
e '- In Eukaryoten ist die Termination der Transkription
|
650
|
+
eng gekoppelt mit dem <b>Poly-Adenylierungssignal</b>.'
|
651
|
+
br
|
652
|
+
e '- Hfq acts as a RNA chaperone.'
|
653
|
+
br
|
654
|
+
e '- Die Behinderung der Segregation nur eines Chromosoms
|
655
|
+
vereitelt die Segregation auch aller anderen Chromosomen.'
|
656
|
+
br
|
657
|
+
e '- Overlapping genes are coupled, meaning that if you
|
658
|
+
manipulate one gene, the other coupled gene might be
|
659
|
+
affected by this change as well. Thus, this constitutes
|
660
|
+
a design constraint.'
|
661
|
+
br
|
662
|
+
e '- RNA-Moleküle haben nur eine begrenzte Lebensdauer
|
663
|
+
("half-life") in der Zelle.'
|
664
|
+
br
|
665
|
+
e '- Exon to Intron comparison revealed that exons happen
|
666
|
+
to have a low "signal-to-noise" ratio in the human genome.'
|
667
|
+
br
|
668
|
+
e '- Exons can be skipped in alternative splicing.'
|
669
|
+
br
|
670
|
+
e '- Die Verteilung des Spo11 Proteins entlang der Chromosomen
|
671
|
+
korreliert mit dem Auftreten meiotischer Doppelstrangbrüche.'
|
672
|
+
br
|
673
|
+
e '- H2AX becomes phosphorylated at sites of DNA-strand
|
674
|
+
breakage.'
|
675
|
+
br
|
676
|
+
e "- Whereas prokaryotic genes are generally accessible and
|
677
|
+
'on' unless repressed, eukaryotic genes are inaccessible
|
678
|
+
and 'off' unless activated."
|
679
|
+
br
|
680
|
+
e '- Transcription is repressed when the promoter is wound
|
681
|
+
up in a nucleosome.'
|
682
|
+
br
|
683
|
+
e '- Ein "scaffold complex" beschleunigt die Reinitiation
|
684
|
+
der DNA-Replikation, die dadurch schneller ablaufen kann.'
|
685
|
+
br
|
686
|
+
e '- In mammals, X chromosome inactivation begins at the
|
687
|
+
XIC (X-Inactivation Center).'
|
688
|
+
br
|
689
|
+
e '- siRNA ultimately helps protect the integrity of
|
690
|
+
genomes.'
|
691
|
+
br
|
692
|
+
e '- A phosphorylation / dephosphorylation cycle controls
|
693
|
+
transription and RNA maturation.'
|
694
|
+
br
|
695
|
+
e '- Die 30nm DNA-Fiber entsteht in dem der Histon-DNA
|
696
|
+
Komplex sich zu zu dieser Faser windet.'
|
697
|
+
br
|
698
|
+
e '- Chiasmen sind die cytologisch sichtbare Folge
|
699
|
+
von Crossovers in Diplotän und Diakinese.'
|
700
|
+
br
|
701
|
+
e '- Eine Chromodomäne kann methylierte Histone erkennen.'
|
702
|
+
br
|
703
|
+
e '- "Crossing over" durchbricht die chromosomale Kopplung
|
704
|
+
von Genen.'
|
705
|
+
br
|
706
|
+
e '- Jede aktive Polymerase führt zur Entstehung einer
|
707
|
+
"<i>transcription bubble</i>".'
|
708
|
+
br
|
709
|
+
e '- XIC constitutes a genomic locus for non-coding RNAs
|
710
|
+
that orchestrate the inactivation of extra X chromosomes
|
711
|
+
in mammalian cells.'
|
712
|
+
br
|
713
|
+
e '- Verschiedene Abschnitte im Chromosom werden zu
|
714
|
+
unterschiedlichen Zeiten in der S-Phase repliziert.'
|
715
|
+
br
|
716
|
+
e '- To maintain the stability of a species, replication
|
717
|
+
of the DNA of a given organism must be almost free of
|
718
|
+
error.'
|
719
|
+
br
|
720
|
+
e '- Jedes Bivalent hat mindestens immer ein
|
721
|
+
Crossover-Ereignis, so klein dies auch immer sein
|
722
|
+
mag. Dies ist unbedingt nötig, da ansonsten homologe
|
723
|
+
Chromosomen nicht voneinander segregieren können.'
|
724
|
+
br
|
725
|
+
e '- Bei Kälte mögen Sekundärstrukturen in der RNA zu stabil
|
726
|
+
werden.'
|
727
|
+
br
|
728
|
+
e '- Pol III Promoters are located downstream from
|
729
|
+
the transcription start site.'
|
730
|
+
br
|
731
|
+
e '- Alternatives Splicing führt zur Diversifizierung des
|
732
|
+
Proteoms.'
|
733
|
+
br
|
734
|
+
e '- In eukaryotes, transcription levels are made finely
|
735
|
+
adjustable in a chromatin environment by clustering
|
736
|
+
binding sites into enhancers.'
|
737
|
+
br
|
738
|
+
e '- Epigenetic Mechanisms lead to heritable changes in
|
739
|
+
gene functions which are not based on changes in the
|
740
|
+
DNA Sequence itself.'
|
741
|
+
br
|
742
|
+
e '- <b>IRES</b> are RNA sequences that function like
|
743
|
+
<b>prokaryotic RBS</b>.'
|
744
|
+
br
|
745
|
+
e '- In contrast with the customary picture of DNA as
|
746
|
+
an extended double helix, structural deformation of
|
747
|
+
DNA to bend or fold into a more compact form is the
|
748
|
+
rule rather than an exception.'
|
749
|
+
br
|
750
|
+
e '- Wenn sich neu synthetisierte DNA-Moleküle (die
|
751
|
+
Chromatiden) nicht auf Tochterkerne aufteilen können
|
752
|
+
entstehen polyploide Zellkerne.'
|
753
|
+
br
|
754
|
+
e '- Eine wahrscheinliche Ursache für die Entstehung
|
755
|
+
von Aneuploidien ist die verminderte Cohäsion der
|
756
|
+
Schwesterchromatiden.'
|
757
|
+
br
|
758
|
+
e '- Innerhalb der Chromosomendomänen ("chromosomal
|
759
|
+
territories") im Zellkern ist das Chromatin
|
760
|
+
<b>nicht</b> zufällig angeordnet.'
|
761
|
+
br
|
762
|
+
e '- Ein Sigmafaktor steuert die Fähigkeit der RNA
|
763
|
+
Polymerase Promoter-DNA von anderer DNA zu
|
764
|
+
unterscheiden; man spricht hierbei von
|
765
|
+
"Diskriminierung von DNA Elementen".'
|
766
|
+
br
|
767
|
+
e '- The SWI-SNF complex can <b>reposition nucleosomes</b>
|
768
|
+
in a test-tube assay if ATP is provided as an
|
769
|
+
energy source.'
|
770
|
+
br
|
771
|
+
e '- Elongation, Termination of Transcription and RNA
|
772
|
+
processing are interconnected to one another.'
|
773
|
+
br
|
774
|
+
e '- Incorporation of Histone H3.3 serves as an
|
775
|
+
epigenetic mark for <b>transcriptionally active
|
776
|
+
chromatin</b>.'
|
777
|
+
br
|
778
|
+
e '- Ligand binding of RNA often produces a change in
|
779
|
+
the secondary structure of RNA.'
|
780
|
+
br
|
781
|
+
e '- DNA functions as <b>a template for biological
|
782
|
+
complexity</b>.'
|
783
|
+
br
|
784
|
+
e '- The ribosome behaves like a small, migrating
|
785
|
+
factory.'
|
786
|
+
br
|
787
|
+
e '- mRNA nonsense mutation may trigger degradation of mRNA.'
|
788
|
+
br
|
789
|
+
e '- mRNA dient als Template für protein synthesis und
|
790
|
+
wird als Ribonucleprotein exportiert.'
|
791
|
+
br
|
792
|
+
e '- Signaling Pathways are the superhighways of the cell.'
|
793
|
+
br
|
794
|
+
e '- All tRNAs must conform to the same structure (in 3D).'
|
795
|
+
br
|
796
|
+
e '- Nucleosomes are inhibitors of transcription because
|
797
|
+
DNA is packed very tightly and is not accessible for
|
798
|
+
RNA polymerases while condensed.'
|
799
|
+
br
|
800
|
+
e '- DNA Melting im Zuge der Transkription geschieht am
|
801
|
+
-10 Bereich; somit ist die -10 Region wichtiger als die
|
802
|
+
-35 Region in Bezug auf die Genexpression.'
|
803
|
+
br
|
804
|
+
e '- tRNA-Synthetases provide a docking platform for
|
805
|
+
aminoacids and their cognate tRNAs.'
|
806
|
+
br
|
807
|
+
e '- TFIIH controls the ATP-dependent transition of the
|
808
|
+
preinitiation complex to the open complex.'
|
809
|
+
br
|
810
|
+
e '- Sobald mehr als 10 Nukleotide synthetisiert worden
|
811
|
+
sind, escaped die Polymerase vom Promoter.'
|
812
|
+
br
|
813
|
+
e '- Nucleosomes are an invariant component of euchromatic
|
814
|
+
and heterochromatin in the interphase nucles and of
|
815
|
+
mitotic chromosomes.'
|
816
|
+
br
|
817
|
+
e '- Heterochromatin can serve as a defense mechanism
|
818
|
+
against foreign DNA, by silencing transposons.'
|
819
|
+
br
|
820
|
+
e '- Point mutations in the promoter and promoter-proximal
|
821
|
+
elements hinder transcription of the Beta-globin gene.'
|
822
|
+
br
|
823
|
+
e '- LCRs und Insulator-Sequenzen können transkribierte
|
824
|
+
und nicht-transkribierte Bereiche durch die Bildung von
|
825
|
+
"chromatin-hubs" voneinander trennen.'
|
826
|
+
br
|
827
|
+
e '- Histone N-terminal tails provide a molecular handle
|
828
|
+
to manipulate DNA accessibility in chromatin.'
|
829
|
+
br
|
830
|
+
e '- CTD Phosphorylierung bestimmt bestimmen wann
|
831
|
+
Proteine an den Polymerasekomplex binden können im
|
832
|
+
Zuge der Transkription.'
|
833
|
+
br
|
834
|
+
e '- Barrier insulators prevent the spreading of
|
835
|
+
heterochromatin by creating a local environment
|
836
|
+
unfavourable to heterochromatin formation.'
|
837
|
+
br
|
838
|
+
e '- Inactivation spreads from heterochromatin
|
839
|
+
into the adjacent region for a variable distance.'
|
840
|
+
br
|
841
|
+
e '- Bei <b>Translokations-Trisomien</b> ist der Grund für
|
842
|
+
die Entstehung eines aneuploiden Gameten eine Fusion
|
843
|
+
von Chromosomen, gefolgt von einem meiotischen
|
844
|
+
Paarungs- und Segregations-Defekt des in der Regel
|
845
|
+
kleineren Fusionspartners.'
|
846
|
+
br
|
847
|
+
e '- A barrier insulator can recruit HATs (Histone
|
848
|
+
Acetyltransferases) and, in doing so, ensure that
|
849
|
+
the adjacent histones are hyperacetylated.'
|
850
|
+
br
|
851
|
+
e '- Historically DNA seemed to be far too simple and
|
852
|
+
too unspecific to support complex biological functions.'
|
853
|
+
br
|
854
|
+
e '- The stereochemical fit between A=T and C☰G in
|
855
|
+
dsDNA is mediated by hydrogen bonds.'
|
856
|
+
br
|
857
|
+
e '- Sister chromatids are held together by the multi-subunit
|
858
|
+
protein complex called <b>cohesin</b>.'
|
859
|
+
br
|
860
|
+
e 'Shugoshin protects cohesin from being degraded by
|
861
|
+
separase at the centromeric regions.'
|
862
|
+
br
|
863
|
+
e '- Genetic information is ultimately equivalent to
|
864
|
+
biochemical function.'
|
865
|
+
br
|
866
|
+
e '- Molecular genes are not solely confined to being separate "units"
|
867
|
+
of inheritance. More importantly they act as a complex assemblages
|
868
|
+
of diverse activating and inhibiting mechanisms, acting in concert
|
869
|
+
to transcribe and translate DNA templates of various makeup into
|
870
|
+
functional protein products.'
|
871
|
+
br
|
872
|
+
e '- Genetics proceeds from the realm of DNA and its
|
873
|
+
replication, to the realm of its transcription, and
|
874
|
+
finally to that of its translation.'
|
875
|
+
br
|
876
|
+
e '- The molecular gene is not defined by its physical and
|
877
|
+
chemical materiality on the level of DNA, but rather by
|
878
|
+
the products that result from its activity.'
|
879
|
+
br
|
880
|
+
e '- The capacity for autocatalysis is inherent not only in
|
881
|
+
"ordinary" genes but in other DNA elements as well, thus
|
882
|
+
questioning the original (historic) definition of the gene
|
883
|
+
concept.'
|
884
|
+
br
|
885
|
+
e '- In 1956, Arthur Kornberg isolated the first DNA polymerase.'
|
886
|
+
br
|
887
|
+
e '- Gene technology allowed genetic engineers to modify model
|
888
|
+
organism with new characteristics. Bacteria could serve as
|
889
|
+
some kind of bioreactor.'
|
890
|
+
br
|
891
|
+
e '- By virtue of being patented, genes additionally assumed
|
892
|
+
the character of commodities.'
|
893
|
+
br
|
894
|
+
e '- The Human Genome Project (HGP) stressed the
|
895
|
+
detrimental, but curable role of genes in disease
|
896
|
+
for its promotion.'
|
897
|
+
br
|
898
|
+
e '- Efforts to automate DNA sequencing began in the
|
899
|
+
early 1980s.'
|
900
|
+
br
|
901
|
+
e '- The promise of medical salvation was very effective
|
902
|
+
in convincing politicians to support the Human Genome
|
903
|
+
Project.'
|
904
|
+
br
|
905
|
+
e '- An example for genetic polmorphism is the fact that
|
906
|
+
the genomes of different human individuals exhibit
|
907
|
+
considerable differences.'
|
908
|
+
br
|
909
|
+
e '- Some epigenetic factors can be named as an example
|
910
|
+
that allows for information to pass from the environment
|
911
|
+
to the genome, at the least in some organisms.'
|
912
|
+
br
|
913
|
+
e '- The pax6 gene product is multifunctional as well as
|
914
|
+
redundant, to some extent. The Drosophila genome, for
|
915
|
+
instance, contains several isoforms with virtually identical
|
916
|
+
effects on eye formation.'
|
917
|
+
br
|
918
|
+
e '- Not every feature of a genome may carry some function
|
919
|
+
that is essential to the survival of the organism.'
|
920
|
+
br
|
921
|
+
e '- The sequences that are annotated as "genes" represent
|
922
|
+
only one set of the components of complex genomic ensembles.
|
923
|
+
Thus, what is a gene is not necessarily an intrinsic property
|
924
|
+
of the DNA sequence at hand, but subject to interpretation
|
925
|
+
and evaluation by the genetic "decipher" system in use, by
|
926
|
+
the corresponding cell at hand.'
|
927
|
+
br
|
928
|
+
e '- In regards to genetic mutations that affect an individual
|
929
|
+
human being negatively, we can state that each embryo is
|
930
|
+
(indirectly) playing "a game of genomic Russian roulette".'
|
931
|
+
br
|
932
|
+
e '- Many of these diseases that will afflict children are
|
933
|
+
knowable in advance, before a couple even conceives.'
|
934
|
+
br
|
935
|
+
e '- Most genomic changes outside of protein-coding regions are
|
936
|
+
not incompatible with vital (living) functions of a complex
|
937
|
+
organism - such as a human being - at hand.'
|
938
|
+
br
|
939
|
+
e '- Genetics is a form of information science.'
|
940
|
+
br
|
941
|
+
e '- The old blending theory of inheritance in genetics can be
|
942
|
+
roughly summarized as "the belief that inheritance worked like
|
943
|
+
the mixing of fluids, such as paints".'
|
944
|
+
br
|
945
|
+
e '- Genetic defects can create chemical individuality among
|
946
|
+
humans.'
|
947
|
+
br
|
948
|
+
e '- The shorter the generation time, the sooner the experiments
|
949
|
+
can be completed.'
|
950
|
+
br
|
951
|
+
e '- An ethical dilemma in regards to engineering human beings
|
952
|
+
in the future may be that any genetically engineered,
|
953
|
+
homogeneous children will be scrubbed of genomic individuality.'
|
954
|
+
br
|
955
|
+
e '- It would be a great feat of engineering to reduce the
|
956
|
+
burden of genetic disease while preserving the genomic
|
957
|
+
diversity of the human species.'
|
958
|
+
br
|
959
|
+
e '- Genetic variation is any difference between two copies of
|
960
|
+
the same gene or DNA molecule.'
|
961
|
+
br
|
962
|
+
e '- In vitro fertilization (IVF) could be used to eliminate
|
963
|
+
(some) genetic disease, such as via <b>pre-implantation
|
964
|
+
genetic testing</b> (PGT).'
|
965
|
+
br
|
966
|
+
e '- Genomic design in humans, aside from ethical considerations
|
967
|
+
in regards to similar problems faced by eugenics, may considerably
|
968
|
+
reduce the burden of recessive genetic diseases and some forms
|
969
|
+
of dominant genetic disease.'
|
970
|
+
br
|
971
|
+
e '- Fathers contribute four times as many new mutations to their
|
972
|
+
offspring as do mothers.'
|
973
|
+
br
|
974
|
+
e '- Intronic sequences found in DNA are spliced out to produce a
|
975
|
+
mature mRNA.'
|
976
|
+
br
|
977
|
+
e '- Mitochondrial disorders can result from alterations in nuclear
|
978
|
+
or mitochondrial genes.'
|
979
|
+
br
|
980
|
+
e '- Mutants allow a geneticist to <i>zero in on any genetic
|
981
|
+
property</i>.'
|
982
|
+
br
|
983
|
+
e '- Translocation events can disrupt critical and essential genes.'
|
984
|
+
br
|
985
|
+
e "- Biological organisms can be viewed as nature's versatile
|
986
|
+
engines of creation."
|
987
|
+
br
|
988
|
+
e '- In haploids, all alleles are expressed in the phenotype
|
989
|
+
because there is no masking of recessives by dominant alleles
|
990
|
+
on the other homolog.'
|
991
|
+
br
|
992
|
+
e '- In a diploid organism, a sterile recessive mutant can be
|
993
|
+
propagated as a heterozygote.'
|
994
|
+
br
|
995
|
+
e '- Genetics is to turn the inorganic into the organic.'
|
996
|
+
br
|
997
|
+
e '- Alternatives Spleißen vermehrt die Codierungsmöglichkeiten
|
998
|
+
eines Gens.'
|
999
|
+
br
|
1000
|
+
e '- Genetics, respectively genomics, is <b>the tale of the
|
1001
|
+
genome</b>.'
|
1002
|
+
br
|
1003
|
+
e '- The story of DNA and RNA replication is that of
|
1004
|
+
<b>complementary surfaces</b>.'
|
1005
|
+
br
|
1006
|
+
e '- Diagnostic breast cancer genetic diagnostics, such as for
|
1007
|
+
the BRCA1 and BRCA2 gene, is owned by <b>Myriad Genetics</b>.'
|
1008
|
+
br
|
1009
|
+
e '- With the expression <b>a gene for a disease</b> we typically
|
1010
|
+
refer to a <b>mutation</b> in a gene that would plays a role in
|
1011
|
+
a specific disease.'
|
1012
|
+
br
|
1013
|
+
e '- Some geneticists may say that <b>the genes may load the
|
1014
|
+
gun but the environment pulls the trigger</b>.'
|
1015
|
+
br
|
1016
|
+
e '- Different RNA molecules replicate at differing rates, at the
|
1017
|
+
least in a test tube. The logical implication of this is that
|
1018
|
+
descendants of the swifter replicators naturally grow more
|
1019
|
+
common in a population.'
|
1020
|
+
br
|
1021
|
+
e '- Genes resemble manuscripts written in a four-letter alphabet.'
|
1022
|
+
br
|
1023
|
+
e '- Genes built ever more elaborate survival machines to aid
|
1024
|
+
their own replication.'
|
1025
|
+
br
|
1026
|
+
e '- RNA replicating in test tubes shows how the idea of purpose
|
1027
|
+
can apply to "mindless molecules".'
|
1028
|
+
br
|
1029
|
+
e '- Genetik ist die universelle biologische Disziplin.'
|
1030
|
+
br
|
1031
|
+
e '- DNA is at the center of the mystery of heredity.'
|
1032
|
+
br
|
1033
|
+
e '- Genetic evolution prefers to refine and extend existing
|
1034
|
+
information rather than create genuinely new information.'
|
1035
|
+
br
|
1036
|
+
e '- Genetic engineering yields the power to shape life.'
|
1037
|
+
br
|
1038
|
+
e '- Erbliche Eigenschaften legen einen Bereich fest, in dem
|
1039
|
+
<b>Variabilität</b> möglich ist.'
|
1040
|
+
br
|
1041
|
+
e '- Synthesized mRNA is interesting because it represents
|
1042
|
+
all active genes of a given cell or tissue. It also forms
|
1043
|
+
the basis for the creation of <b>cDNA libraries</b>.'
|
1044
|
+
br
|
1045
|
+
e '- The <b>telomerase</b> is <b>an unusual type of reverse
|
1046
|
+
transcriptase</b>.'
|
1047
|
+
br
|
1048
|
+
e '- Sequences in regions adjacent to the gene itself
|
1049
|
+
are called <b>cis-acting elements</b>.'
|
1050
|
+
br
|
1051
|
+
e '- Die komplexere Organisation eines Organismus schlägt
|
1052
|
+
sich jedoch in einer höheren Anzahl von Proteinen nieder.'
|
1053
|
+
br
|
1054
|
+
}
|
1055
|
+
# ========================================================================= #
|
1056
|
+
# === Links
|
1057
|
+
# ========================================================================= #
|
1058
|
+
h2 'Links (remote)',
|
1059
|
+
'mart1px'
|
1060
|
+
dot104 = dot104?
|
1061
|
+
p('mars1em') {
|
1062
|
+
abr 'https://www.onmeda.de/krankheiten/krankheiten_az.html',
|
1063
|
+
content: dot104+'Verschiedene Krankheiten (auf onmeda.de)',
|
1064
|
+
css_class: 'slateblue'
|
1065
|
+
abr 'https://www.embl.org/sites/heidelberg/',
|
1066
|
+
content: dot104+'EMBL in Heidelberg',
|
1067
|
+
css_class: 'slateblue'
|
1068
|
+
}
|
1069
|
+
# ========================================================================= #
|
1070
|
+
# === Local Links tag
|
1071
|
+
# ========================================================================= #
|
1072
|
+
h2 sg(:square_yellow, 'bblack1 marr4px')+
|
1073
|
+
'Local Links',
|
1074
|
+
'martb2px'
|
1075
|
+
div('default martb1px') {
|
1076
|
+
p('ind0px marl2em mart1px padt1px') {
|
1077
|
+
abr_self :local_science
|
1078
|
+
}
|
1079
|
+
}
|
1080
|
+
}}
|