qu-pcr 1.0.9 → 1.0.12

This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
Files changed (6) hide show
  1. checksums.yaml +4 -4
  2. data/.gitignore +1 -0
  3. data/lib/qu/pcr/primer3.rb +21 -20
  4. data/lib/qu/pcr/primer3_parameters.rb +188 -73
  5. data/lib/qu/pcr/version.rb +1 -1
  6. metadata +3 -3
checksums.yaml CHANGED
@@ -1,7 +1,7 @@
1
1
  ---
2
2
  SHA1:
3
- metadata.gz: e789378488b5828518e32ba64d3471f2e9074fdf
4
- data.tar.gz: e744ab317a3f6f88174fb55538582067b2610a14
3
+ metadata.gz: d0463909bfb89c89aa7739042805062be342224e
4
+ data.tar.gz: 32dccb0916f92057097f2dc90c04ccfad0fd380a
5
5
  SHA512:
6
- metadata.gz: cd0bf147438ec621fb18ebd141ffbe4d8f8f25fd17ba07d1cd9744d33cc0fb5402294b4451e88c7599a3546aad70331ebbdd550c4af7262d5f0c5885ca1d2731
7
- data.tar.gz: 7bd7018d531e1d21baa8776ce7a28517f83c8d1c2bf6f2951c63315dce1b74ab8440243e7cdcaffc71fff2aa75456def1e53b33693ecd3ba9b1a6e3070727077
6
+ metadata.gz: 091ecb385b0aa540fb8032ff13cefd28c370a0714cbd7b61484411dc6f1167f9d37a2345ef7b6b5524a7f3656cf591887b40a767c5b50479f63165fa828f0937
7
+ data.tar.gz: c470f7dbadcde3df10789aba3ca77b7fafa6d5996eb508913694cf444b11a528b01abf45de6f65b8d5078f1f16c314345b06e0308ef4e90e4c969aba3e89fb85
data/.gitignore CHANGED
@@ -15,3 +15,4 @@ spec/reports
15
15
  test/tmp
16
16
  test/version_tmp
17
17
  tmp
18
+ .DS_Store
data/lib/qu/pcr/primer3.rb CHANGED
@@ -25,32 +25,15 @@ module Qu
25
25
  end
26
26
 
27
27
  def out
28
- p3_string = create_p3_input()
29
- p3_input_file = Tempfile.new('p3_input')
30
- p3_input_file.write(p3_string)
31
- p3_input_file.close
32
-
33
- p3_out = Cmdwrapper::primer3_core(p3_input_file)
34
-
35
- p3_input_file.unlink
28
+ p3_out = Cmdwrapper::primer3_core(@para)
36
29
 
37
30
  return Primer3Parser.new(p3_out).records
38
31
  end
39
32
 
40
- private
41
- def create_p3_input()
42
- lines = []
43
- @para.each_pair do |key, value|
44
- next if value.to_s.empty?
45
- lines << "#{key}=#{value}"
46
- end
47
- lines << "="
48
- return lines.join("\n")
49
- end
50
33
  end # End Primer3 class
51
34
 
52
35
  class Amplicon
53
- attr_reader :fp, :rp, :product
36
+ attr_reader :fp, :rp, :probe, :product
54
37
  def initialize(record, i, type='primer3')
55
38
  if type == 'primer3'
56
39
  (fp_5_pos, fp_size) = record["PRIMER_LEFT_%s" %[i]].split(',')
@@ -70,6 +53,24 @@ module Qu
70
53
  id = record["SEQUENCE_ID"].to_s
71
54
  product_seq = record["SEQUENCE_TEMPLATE"].to_s[fp_5_pos.to_i..rp_5_pos.to_i]
72
55
 
56
+ if record.has_key?("PRIMER_INTERNAL_NUM_RETURNED") && record["PRIMER_INTERNAL_NUM_RETURNED"].to_i > 0
57
+ (probe_5_pos, probe_size) = record["PRIMER_INTERNAL_%s" %[i]].split(',')
58
+ probe_seq = record["PRIMER_INTERNAL_%s_SEQUENCE" % [i]]
59
+ probe_gc = record["PRIMER_INTERNAL_%s_GC_PERCENT" % [i]].to_f
60
+ probe_tm = record["PRIMER_INTERNAL_%s_TM" % [i]].to_f
61
+ probe_penalty = record["PRIMER_INTERNAL_%s_PENALTY" % [i]].to_f
62
+
63
+ @probe = Primer.new(seq = probe_seq,
64
+ type = 'probe',
65
+ gc = probe_gc,
66
+ tm = probe_tm,
67
+ penalty = probe_penalty,
68
+ pos = probe_5_pos,
69
+ )
70
+ else
71
+ @probe = nil
72
+ end
73
+
73
74
  @fp = Primer.new(seq = fp_seq,
74
75
  type = 'forward',
75
76
  gc = fp_gc,
@@ -139,8 +140,8 @@ module Qu
139
140
  $stderr.puts "\tFailed design for %s" % [seq_id]
140
141
  $stderr.puts "\tLeft: #{record['PRIMER_LEFT_EXPLAIN']}"
141
142
  $stderr.puts "\tRight: #{record['PRIMER_RIGHT_EXPLAIN']}"
143
+ $stderr.puts "\INTERNAL: #{record['PRIMER_INTERNAL_EXPLAIN']}" if record.has_key?("PRIMER_INTERNAL_EXPLAIN")
142
144
  $stderr.puts "\tPair: #{record['PRIMER_PAIR_EXPLAIN']}"
143
- #exit
144
145
  end
145
146
  p3_hash[seq_id] = []
146
147
  (0...pp_num).each do |i|
data/lib/qu/pcr/primer3_parameters.rb CHANGED
@@ -3,83 +3,198 @@
3
3
  module Qu
4
4
  module Pcr
5
5
  PRIMER3_OPTIONS = {
6
- SEQUENCE_ID: "",
7
- SEQUENCE_TARGET: "",
8
- SEQUENCE_EXCLUDED_REGION: "",
9
- SEQUENCE_FORCE_LEFT_START: -1000000,
10
- SEQUENCE_FORCE_LEFT_END: -1000000,
11
- SEQUENCE_FORCE_RIGHT_START: -1000000,
12
- SEQUENCE_FORCE_RIGHT_END: -1000000,
13
- SEQUENCE_PRIMER: '',
14
- SEQUENCE_PRIMER_REVCOMP: '',
15
- PRIMER_PRODUCT_SIZE_RANGE: '100-200 200-300 300-400 400-500',
16
- PRIMER_MIN_SIZE: 18,
17
- PRIMER_OPT_SIZE: 22,
18
- PRIMER_MAX_SIZE: 28,
19
- PRIMER_MIN_TM: 55,
20
- PRIMER_OPT_TM: 60,
21
- PRIMER_MAX_TM: 65,
22
- PRIMER_MIN_GC: 40,
23
- PRIMER_OPT_GC: 50,
24
- PRIMER_MAX_GC: 60,
25
- PRIMER_MAX_POLY_X: 5,
26
- PRIMER_MAX_HAIRPIN_TH: 47,
27
- PRIMER_MAX_SELF_ANY_TH: 47,
28
- PRIMER_MAX_SELF_END_TH: 47,
29
- PRIMER_PAIR_MAX_COMPL_ANY_TH: 47,
30
- PRIMER_PAIR_MAX_COMPL_END_TH: 47,
31
- SEQUENCE_TEMPLATE: "",
32
- PRIMER_THERMODYNAMIC_PARAMETERS_PATH: Cmdwrapper::THERMO_PATH,
33
- PRIMER_PICK_LEFT_PRIMER: 1,
34
- PRIMER_PICK_INTERNAL_OLIGO: 0,
35
- PRIMER_PICK_RIGHT_PRIMER: 1,
36
- PRIMER_NUM_RETURN: 10,
37
- PRIMER_MAX_NS_ACCEPTED: 0,
38
- # PRIMER_THERMODYNAMIC_ALIGNMENT: 1, # not available since release 2.3.5 2013-01-03
39
- PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT: 1,
40
- PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT: 1,
41
- PRIMER_TM_FORMULA: 1,
42
- PRIMER_SALT_CORRECTIONS: 1,
43
- PRIMER_SALT_MONOVALENT: 50.0,
44
- PRIMER_SALT_DIVALENT: 1.5,
45
- PRIMER_DNTP_CONC: 0.25,
46
- PRIMER_DNA_CONC: 50.0,
47
- P3_FILE_FLAG: 0,
48
- PRIMER_EXPLAIN_FLAG: 1,
49
- PRIMER_PRODUCT_MAX_TM: 100000,
50
- PRIMER_PICK_ANYWAY: 0,
51
- PRIMER_TASK: "generic",
52
- SEQUENCE_PRIMER_PAIR_OK_REGION_LIST: "",
53
- PRIMER_MIN_THREE_PRIME_DISTANCE: 1,
54
- PRIMER_LOWERCASE_MASKING: 1,
55
- PRIMER_PRODUCT_OPT_TM: 0,
6
+ SEQUENCE_ID: "",
7
+ SEQUENCE_TEMPLATE: "",
8
+ SEQUENCE_INCLUDED_REGION: "",
9
+ SEQUENCE_TARGET: "",
10
+ SEQUENCE_EXCLUDED_REGION: "",
11
+ SEQUENCE_PRIMER_PAIR_OK_REGION_LIST: "",
12
+ SEQUENCE_OVERLAP_JUNCTION_LIST: "",
13
+ SEQUENCE_INTERNAL_EXCLUDED_REGION: "",
14
+ SEQUENCE_PRIMER: "",
15
+ SEQUENCE_INTERNAL_OLIGO: "",
16
+ SEQUENCE_PRIMER_REVCOMP: "",
17
+ SEQUENCE_FORCE_LEFT_START: -1000000,
18
+ SEQUENCE_FORCE_LEFT_END: -1000000,
19
+ SEQUENCE_FORCE_RIGHT_START: -1000000,
20
+ SEQUENCE_FORCE_RIGHT_END: -1000000,
21
+
22
+ PRIMER_TASK: "generic",
23
+ PRIMER_PICK_LEFT_PRIMER: 1,
24
+ PRIMER_PICK_INTERNAL_OLIGO: 0,
25
+ PRIMER_PICK_RIGHT_PRIMER: 1,
26
+ PRIMER_NUM_RETURN: 10,
27
+ PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION: 4,
28
+ PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION: 7,
29
+ PRIMER_MUST_MATCH_FIVE_PRIME: "",
30
+ PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME: "",
31
+ PRIMER_MUST_MATCH_THREE_PRIME: "",
32
+ PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME: "",
33
+ PRIMER_PRODUCT_SIZE_RANGE: "100-150 151-250 100-300 301-400 401-500 501-600 601-700 701-850 851-1000",
34
+ PRIMER_PRODUCT_OPT_SIZE: 0,
35
+ PRIMER_PAIR_WT_PRODUCT_SIZE_LT: 0.0,
36
+ PRIMER_PAIR_WT_PRODUCT_SIZE_GT: 0.0,
37
+ PRIMER_MIN_SIZE: 15,
38
+ PRIMER_INTERNAL_MIN_SIZE: 18,
39
+ PRIMER_OPT_SIZE: 22,
40
+ PRIMER_INTERNAL_OPT_SIZE: 20,
41
+ PRIMER_MAX_SIZE: 35,
42
+ PRIMER_INTERNAL_MAX_SIZE: 35,
43
+ # Customed by Wubin Qu
44
+ PRIMER_WT_SIZE_LT: 0.0,
45
+ PRIMER_INTERNAL_WT_SIZE_LT: 0.0,
46
+ # Customed by Wubin Qu
47
+ PRIMER_WT_SIZE_GT: 0.0,
48
+ PRIMER_INTERNAL_WT_SIZE_GT: 0.0,
49
+ PRIMER_MIN_GC: 20.0,
50
+ PRIMER_INTERNAL_MIN_GC: 20.0,
51
+ PRIMER_OPT_GC_PERCENT: 50.0,
52
+ PRIMER_INTERNAL_OPT_GC_PERCENT: 50.0,
53
+ PRIMER_MAX_GC: 80.0,
54
+ PRIMER_INTERNAL_MAX_GC: 80.0,
55
+ PRIMER_WT_GC_PERCENT_LT: 0.0,
56
+ PRIMER_INTERNAL_WT_GC_PERCENT_LT: 0.0,
57
+ PRIMER_WT_GC_PERCENT_GT: 0.0,
58
+ PRIMER_INTERNAL_WT_GC_PERCENT_GT: 0.0,
59
+ PRIMER_GC_CLAMP: 0,
60
+ PRIMER_MAX_END_GC: 5,
61
+ PRIMER_MIN_TM: 55.0,
62
+ PRIMER_INTERNAL_MIN_TM: 55.0,
63
+ PRIMER_OPT_TM: 62.0,
64
+ PRIMER_INTERNAL_OPT_TM: 62.0,
65
+ PRIMER_MAX_TM: 65.0,
66
+ PRIMER_INTERNAL_MAX_TM: 65.0,
67
+ PRIMER_PAIR_MAX_DIFF_TM: 10.0,
68
+ PRIMER_WT_TM_LT: 1.0,
69
+ PRIMER_INTERNAL_WT_TM_LT: 1.0,
70
+ PRIMER_WT_TM_GT: 1.0,
71
+ PRIMER_INTERNAL_WT_TM_GT: 1.0,
72
+ PRIMER_PAIR_WT_DIFF_TM: 1.0,
73
+ PRIMER_PRODUCT_MIN_TM: -1000000.0,
74
+ PRIMER_PRODUCT_OPT_TM: 0.0,
75
+ PRIMER_PRODUCT_MAX_TM: 1000000.0,
76
+ PRIMER_PAIR_WT_PRODUCT_TM_LT: 0.0,
77
+ PRIMER_PAIR_WT_PRODUCT_TM_GT: 0.0,
78
+ PRIMER_TM_FORMULA: 1,
79
+ PRIMER_SALT_MONOVALENT: 50.0,
80
+ PRIMER_INTERNAL_SALT_MONOVALENT: 50.0,
81
+ PRIMER_SALT_DIVALENT: 1.5,
82
+ PRIMER_INTERNAL_SALT_DIVALENT: 0.0,
83
+ # Customed by Wubin Qu
84
+ PRIMER_DNTP_CONC: 0.25,
85
+ PRIMER_INTERNAL_DNTP_CONC: 0.0,
86
+ PRIMER_SALT_CORRECTIONS: 1,
87
+ PRIMER_DNA_CONC: 50.0,
88
+ PRIMER_INTERNAL_DNA_CONC: 50.0,
89
+ PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT: 1,
90
+ PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT: 1,
91
+ PRIMER_THERMODYNAMIC_PARAMETERS_PATH: Cmdwrapper::THERMO_PATH,
92
+ PRIMER_MAX_SELF_ANY: 8.00,
93
+ PRIMER_MAX_SELF_ANY_TH: 47.00,
94
+ PRIMER_INTERNAL_MAX_SELF_ANY: 12.00,
95
+ PRIMER_INTERNAL_MAX_SELF_ANY_TH: 47.00,
96
+ PRIMER_PAIR_MAX_COMPL_ANY: 8.00,
97
+ PRIMER_PAIR_MAX_COMPL_ANY_TH: 47.00,
98
+ PRIMER_WT_SELF_ANY: 0.0,
99
+ PRIMER_WT_SELF_ANY_TH: 0.0,
100
+ PRIMER_INTERNAL_WT_SELF_ANY: 0.0,
101
+ PRIMER_INTERNAL_WT_SELF_ANY_TH: 0.0,
102
+ PRIMER_PAIR_WT_COMPL_ANY: 0.0,
103
+ PRIMER_PAIR_WT_COMPL_ANY_TH: 0.0,
104
+ PRIMER_MAX_SELF_END: 3.0,
105
+ PRIMER_MAX_SELF_END_TH: 47.00,
106
+ PRIMER_INTERNAL_MAX_SELF_END: 12.00,
107
+ PRIMER_INTERNAL_MAX_SELF_END_TH: 47.00,
108
+ PRIMER_PAIR_MAX_COMPL_END: 3.00,
109
+ PRIMER_PAIR_MAX_COMPL_END_TH: 47.00,
110
+ PRIMER_WT_SELF_END: 0.0,
111
+ PRIMER_WT_SELF_END_TH: 0.0,
112
+ PRIMER_INTERNAL_WT_SELF_END: 0.0,
113
+ PRIMER_INTERNAL_WT_SELF_END_TH: 0.0,
114
+ PRIMER_PAIR_WT_COMPL_END: 0.0,
115
+ PRIMER_PAIR_WT_COMPL_END_TH: 0.0,
116
+ PRIMER_MAX_HAIRPIN_TH: 47.0,
117
+ PRIMER_INTERNAL_MAX_HAIRPIN_TH: 47.0,
118
+ PRIMER_WT_HAIRPIN_TH: 0.0,
119
+ PRIMER_INTERNAL_WT_HAIRPIN_TH: 0.0,
120
+ PRIMER_MAX_END_STABILITY: 100.0,
121
+ PRIMER_WT_END_STABILITY: 0.0,
122
+ PRIMER_MAX_NS_ACCEPTED: 0,
123
+ PRIMER_INTERNAL_MAX_NS_ACCEPTED: 0,
124
+ PRIMER_WT_NUM_NS: 0.0,
125
+ PRIMER_INTERNAL_WT_NUM_NS: 0.0,
126
+ PRIMER_MAX_POLY_X: 5,
127
+ PRIMER_INTERNAL_MAX_POLY_X: 5,
128
+ PRIMER_MIN_THREE_PRIME_DISTANCE: 1,
129
+ PRIMER_PICK_ANYWAY: 0,
130
+ PRIMER_LOWERCASE_MASKING: 1,
131
+ PRIMER_EXPLAIN_FLAG: 1,
132
+ PRIMER_LIBERAL_BASE: 0,
133
+ PRIMER_FIRST_BASE_INDEX: 0,
134
+ PRIMER_MAX_TEMPLATE_MISPRIMING: -1.00,
135
+ PRIMER_MAX_TEMPLATE_MISPRIMING_TH: -1.00,
136
+ PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING: -1.00,
137
+ PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH: -1.00,
138
+ PRIMER_WT_TEMPLATE_MISPRIMING: 0.0,
139
+ PRIMER_WT_TEMPLATE_MISPRIMING_TH: 0.0,
140
+ PRIMER_PAIR_WT_TEMPLATE_MISPRIMING: 0.0,
141
+ PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH: 0.0,
142
+ PRIMER_MISPRIMING_LIBRARY: "",
143
+ PRIMER_INTERNAL_MISHYB_LIBRARY: "",
144
+ PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS: 0,
145
+ PRIMER_MAX_LIBRARY_MISPRIMING: 12.00,
146
+ PRIMER_INTERNAL_MAX_LIBRARY_MISHYB: 12.00,
147
+ PRIMER_PAIR_MAX_LIBRARY_MISPRIMING: 24.00,
148
+ PRIMER_WT_LIBRARY_MISPRIMING: 0.0,
149
+ PRIMER_INTERNAL_WT_LIBRARY_MISHYB: 0.0,
150
+ PRIMER_PAIR_WT_LIBRARY_MISPRIMING: 0.0,
151
+ PRIMER_MIN_QUALITY: 0,
152
+ PRIMER_INTERNAL_MIN_QUALITY: 0,
153
+ PRIMER_MIN_END_QUALITY: 0,
154
+ PRIMER_QUALITY_RANGE_MIN: 0,
155
+ PRIMER_QUALITY_RANGE_MAX: 100,
156
+ PRIMER_WT_SEQ_QUAL: 0.0,
157
+ PRIMER_INTERNAL_WT_SEQ_QUAL: 0.0,
158
+ PRIMER_PAIR_WT_PR_PENALTY: 1.0,
159
+ PRIMER_PAIR_WT_IO_PENALTY: 0.0,
160
+ PRIMER_INSIDE_PENALTY: -1.0,
161
+ PRIMER_OUTSIDE_PENALTY: 0.0,
162
+ PRIMER_WT_POS_PENALTY: 1.0,
163
+ PRIMER_SEQUENCING_LEAD: 50,
164
+ PRIMER_SEQUENCING_SPACING: 500,
165
+ PRIMER_SEQUENCING_INTERVAL: 250,
166
+ PRIMER_SEQUENCING_ACCURACY: 20,
167
+ PRIMER_WT_END_QUAL: 0.0,
168
+ PRIMER_INTERNAL_WT_END_QUAL: 0.0,
169
+
170
+ P3_FILE_FLAG: 0,
56
171
  }
57
172
 
58
173
  # Parameters not shown to users for editing
59
174
  USER_SPECIFIC_OPTIONS = [
60
- :SEQUENCE_ID,
61
- :SEQUENCE_TARGET,
62
- :SEQUENCE_EXCLUDED_REGION,
63
- :SEQUENCE_FORCE_LEFT_START,
64
- :SEQUENCE_FORCE_LEFT_END,
65
- :SEQUENCE_FORCE_RIGHT_START,
66
- :SEQUENCE_FORCE_RIGHT_END,
67
- :SEQUENCE_PRIMER,
68
- :SEQUENCE_PRIMER_REVCOMP,
69
- :PRIMER_PRODUCT_SIZE_RANGE,
70
- :PRIMER_MIN_SIZE,
71
- :PRIMER_OPT_SIZE,
72
- :PRIMER_MAX_SIZE,
73
- :PRIMER_MIN_TM,
74
- :PRIMER_OPT_TM,
75
- :PRIMER_MAX_TM,
76
- :PRIMER_MIN_GC,
77
- :PRIMER_OPT_GC,
78
- :PRIMER_MAX_GC,
79
- :PRIMER_MAX_POLY_X,
80
- :PRIMER_MAX_HAIRPIN_TH, # I think it's no needed here, becuse they should be checked in MFEprimer (>v3.0), Wubin Qu [2014-5-8]
81
- :PRIMER_MAX_SELF_ANY_TH,
82
- :PRIMER_MAX_SELF_END_TH,
175
+ :SEQUENCE_ID,
176
+ :SEQUENCE_TARGET,
177
+ :SEQUENCE_EXCLUDED_REGION,
178
+ :SEQUENCE_FORCE_LEFT_START,
179
+ :SEQUENCE_FORCE_LEFT_END,
180
+ :SEQUENCE_FORCE_RIGHT_START,
181
+ :SEQUENCE_FORCE_RIGHT_END,
182
+ :SEQUENCE_PRIMER,
183
+ :SEQUENCE_PRIMER_REVCOMP,
184
+ :PRIMER_PRODUCT_SIZE_RANGE,
185
+ :PRIMER_MIN_SIZE,
186
+ :PRIMER_OPT_SIZE,
187
+ :PRIMER_MAX_SIZE,
188
+ :PRIMER_MIN_TM,
189
+ :PRIMER_OPT_TM,
190
+ :PRIMER_MAX_TM,
191
+ :PRIMER_MIN_GC,
192
+ :PRIMER_OPT_GC,
193
+ :PRIMER_MAX_GC,
194
+ :PRIMER_MAX_POLY_X,
195
+ :PRIMER_MAX_HAIRPIN_TH, # I think it's no needed here, becuse they should be checked in MFEprimer (>v3.0), Wubin Qu [2014-5-8]
196
+ :PRIMER_MAX_SELF_ANY_TH,
197
+ :PRIMER_MAX_SELF_END_TH,
83
198
  :PRIMER_PAIR_MAX_COMPL_ANY_TH,
84
199
  :PRIMER_PAIR_MAX_COMPL_END_TH,
85
200
  :PRIMER_PRODUCT_OPT_TM,
data/lib/qu/pcr/version.rb CHANGED
@@ -1,5 +1,5 @@
1
1
  module Qu
2
2
  module Pcr
3
- VERSION = "1.0.9"
3
+ VERSION = "1.0.12"
4
4
  end
5
5
  end
metadata CHANGED
@@ -1,14 +1,14 @@
1
1
  --- !ruby/object:Gem::Specification
2
2
  name: qu-pcr
3
3
  version: !ruby/object:Gem::Version
4
- version: 1.0.9
4
+ version: 1.0.12
5
5
  platform: ruby
6
6
  authors:
7
7
  - Wubin Qu
8
8
  autorequire:
9
9
  bindir: bin
10
10
  cert_chain: []
11
- date: 2014-10-13 00:00:00.000000000 Z
11
+ date: 2014-10-21 00:00:00.000000000 Z
12
12
  dependencies:
13
13
  - !ruby/object:Gem::Dependency
14
14
  name: qu-utils
@@ -104,7 +104,7 @@ required_rubygems_version: !ruby/object:Gem::Requirement
104
104
  version: '0'
105
105
  requirements: []
106
106
  rubyforge_project:
107
- rubygems_version: 2.4.1
107
+ rubygems_version: 2.2.2
108
108
  signing_key:
109
109
  specification_version: 4
110
110
  summary: A ruby library for PCR