miga-base 1.2.17.1 → 1.2.17.2

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Files changed (91) hide show
  1. checksums.yaml +4 -4
  2. data/lib/miga/version.rb +2 -2
  3. data/utils/enveomics/Manifest/Tasks/mapping.json +39 -11
  4. data/utils/enveomics/Manifest/Tasks/remote.json +2 -1
  5. data/utils/enveomics/Scripts/BedGraph.tad.rb +98 -53
  6. data/utils/enveomics/Scripts/SRA.download.bash +14 -2
  7. data/utils/enveomics/Tests/low-cov.bg.gz +0 -0
  8. data/utils/enveomics/enveomics.R/DESCRIPTION +5 -5
  9. data/utils/enveomics/enveomics.R/R/autoprune.R +99 -87
  10. data/utils/enveomics/enveomics.R/R/barplot.R +116 -97
  11. data/utils/enveomics/enveomics.R/R/cliopts.R +65 -59
  12. data/utils/enveomics/enveomics.R/R/df2dist.R +96 -58
  13. data/utils/enveomics/enveomics.R/R/growthcurve.R +166 -148
  14. data/utils/enveomics/enveomics.R/R/recplot.R +201 -136
  15. data/utils/enveomics/enveomics.R/R/recplot2.R +371 -304
  16. data/utils/enveomics/enveomics.R/R/tribs.R +318 -263
  17. data/utils/enveomics/enveomics.R/R/utils.R +30 -20
  18. data/utils/enveomics/enveomics.R/man/enve.TRIBS-class.Rd +4 -3
  19. data/utils/enveomics/enveomics.R/man/enve.TRIBS.merge.Rd +2 -2
  20. data/utils/enveomics/enveomics.R/man/enve.TRIBStest-class.Rd +3 -3
  21. data/utils/enveomics/enveomics.R/man/enve.__prune.iter.Rd +7 -4
  22. data/utils/enveomics/enveomics.R/man/enve.__prune.reduce.Rd +7 -4
  23. data/utils/enveomics/enveomics.R/man/enve.__tribs.Rd +4 -0
  24. data/utils/enveomics/enveomics.R/man/enve.barplot.Rd +25 -17
  25. data/utils/enveomics/enveomics.R/man/enve.col.alpha.Rd +10 -0
  26. data/utils/enveomics/enveomics.R/man/enve.col2alpha.Rd +8 -2
  27. data/utils/enveomics/enveomics.R/man/enve.df2dist.Rd +14 -0
  28. data/utils/enveomics/enveomics.R/man/enve.df2dist.group.Rd +20 -1
  29. data/utils/enveomics/enveomics.R/man/enve.df2dist.list.Rd +2 -3
  30. data/utils/enveomics/enveomics.R/man/enve.growthcurve.Rd +5 -2
  31. data/utils/enveomics/enveomics.R/man/enve.recplot.Rd +50 -42
  32. data/utils/enveomics/enveomics.R/man/enve.recplot2.ANIr.Rd +5 -2
  33. data/utils/enveomics/enveomics.R/man/enve.recplot2.__counts.Rd +3 -0
  34. data/utils/enveomics/enveomics.R/man/enve.recplot2.__peakHist.Rd +3 -0
  35. data/utils/enveomics/enveomics.R/man/enve.recplot2.__whichClosestPeak.Rd +3 -0
  36. data/utils/enveomics/enveomics.R/man/enve.recplot2.changeCutoff.Rd +3 -0
  37. data/utils/enveomics/enveomics.R/man/enve.recplot2.compareIdentities.Rd +9 -4
  38. data/utils/enveomics/enveomics.R/man/enve.recplot2.corePeak.Rd +3 -0
  39. data/utils/enveomics/enveomics.R/man/enve.recplot2.extractWindows.Rd +3 -3
  40. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.Rd +0 -2
  41. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__em_e.Rd +4 -0
  42. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__em_m.Rd +5 -0
  43. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__emauto_one.Rd +11 -7
  44. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__mow_one.Rd +5 -1
  45. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__mower.Rd +3 -0
  46. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.mower.Rd +2 -2
  47. data/utils/enveomics/enveomics.R/man/enve.recplot2.windowDepthThreshold.Rd +3 -3
  48. data/utils/enveomics/enveomics.R/man/enve.tribs.Rd +2 -2
  49. data/utils/enveomics/enveomics.R/man/plot.enve.GrowthCurve.Rd +3 -0
  50. data/utils/enveomics/enveomics.R/man/plot.enve.TRIBS.Rd +3 -0
  51. data/utils/enveomics/enveomics.R/man/plot.enve.TRIBStest.Rd +6 -3
  52. data/utils/enveomics/enveomics.R/man/plot.enve.recplot2.Rd +2 -2
  53. data/utils/enveomics/enveomics.R/man/summary.enve.GrowthCurve.Rd +3 -0
  54. data/utils/enveomics/enveomics.R/man/summary.enve.TRIBS.Rd +3 -0
  55. data/utils/enveomics/enveomics.R/man/summary.enve.TRIBStest.Rd +3 -0
  56. metadata +3 -37
  57. data/utils/enveomics/Pipelines/assembly.pbs/CONFIG.mock.bash +0 -69
  58. data/utils/enveomics/Pipelines/assembly.pbs/FastA.N50.pl +0 -1
  59. data/utils/enveomics/Pipelines/assembly.pbs/FastA.filterN.pl +0 -1
  60. data/utils/enveomics/Pipelines/assembly.pbs/FastA.length.pl +0 -1
  61. data/utils/enveomics/Pipelines/assembly.pbs/README.md +0 -189
  62. data/utils/enveomics/Pipelines/assembly.pbs/RUNME-2.bash +0 -112
  63. data/utils/enveomics/Pipelines/assembly.pbs/RUNME-3.bash +0 -23
  64. data/utils/enveomics/Pipelines/assembly.pbs/RUNME-4.bash +0 -44
  65. data/utils/enveomics/Pipelines/assembly.pbs/RUNME.bash +0 -50
  66. data/utils/enveomics/Pipelines/assembly.pbs/kSelector.R +0 -37
  67. data/utils/enveomics/Pipelines/assembly.pbs/newbler.pbs +0 -68
  68. data/utils/enveomics/Pipelines/assembly.pbs/newbler_preparator.pl +0 -49
  69. data/utils/enveomics/Pipelines/assembly.pbs/soap.pbs +0 -80
  70. data/utils/enveomics/Pipelines/assembly.pbs/stats.pbs +0 -57
  71. data/utils/enveomics/Pipelines/assembly.pbs/velvet.pbs +0 -63
  72. data/utils/enveomics/Pipelines/blast.pbs/01.pbs.bash +0 -38
  73. data/utils/enveomics/Pipelines/blast.pbs/02.pbs.bash +0 -73
  74. data/utils/enveomics/Pipelines/blast.pbs/03.pbs.bash +0 -21
  75. data/utils/enveomics/Pipelines/blast.pbs/BlastTab.recover_job.pl +0 -72
  76. data/utils/enveomics/Pipelines/blast.pbs/CONFIG.mock.bash +0 -98
  77. data/utils/enveomics/Pipelines/blast.pbs/FastA.split.pl +0 -1
  78. data/utils/enveomics/Pipelines/blast.pbs/README.md +0 -127
  79. data/utils/enveomics/Pipelines/blast.pbs/RUNME.bash +0 -109
  80. data/utils/enveomics/Pipelines/blast.pbs/TASK.check.bash +0 -128
  81. data/utils/enveomics/Pipelines/blast.pbs/TASK.dry.bash +0 -16
  82. data/utils/enveomics/Pipelines/blast.pbs/TASK.eo.bash +0 -22
  83. data/utils/enveomics/Pipelines/blast.pbs/TASK.pause.bash +0 -26
  84. data/utils/enveomics/Pipelines/blast.pbs/TASK.run.bash +0 -89
  85. data/utils/enveomics/Pipelines/blast.pbs/sentinel.pbs.bash +0 -29
  86. data/utils/enveomics/Pipelines/idba.pbs/README.md +0 -49
  87. data/utils/enveomics/Pipelines/idba.pbs/RUNME.bash +0 -95
  88. data/utils/enveomics/Pipelines/idba.pbs/run.pbs +0 -56
  89. data/utils/enveomics/Pipelines/trim.pbs/README.md +0 -54
  90. data/utils/enveomics/Pipelines/trim.pbs/RUNME.bash +0 -70
  91. data/utils/enveomics/Pipelines/trim.pbs/run.pbs +0 -130
@@ -1,44 +0,0 @@
1
- #!/bin/bash
2
-
3
- ##################### RUN
4
- # Find the directory of the pipeline
5
- PDIR=$(dirname $(readlink -f $0));
6
- # Load variables
7
- source "$PDIR/RUNME.bash"
8
- if [[ "$SCRATCH" == "" ]] ; then
9
- echo "$0: Error loading $PDIR/RUNME.bash, variable SCRATCH undefined" >&2
10
- exit 1
11
- fi
12
-
13
- # Run it
14
- echo "Jobs being launched in $SCRATCH"
15
- RAMMULT=${RAMMULT:-1}
16
- for LIB in $LIBRARIES; do
17
- # Prepare info
18
- echo "Running $LIB";
19
- K_VELVET=$(echo $K_VELVET | sed -e 's/ /:/g')
20
- K_SOAP=$(echo $K_SOAP | sed -e 's/ /:/g')
21
- if [[ "$USECOUPLED" == "yes" ]] ; then
22
- INPUT="$DATA/$LIB.CoupledReads.fa"
23
- elif [[ "$USESINGLE" == "yes" ]] ; then
24
- INPUT="$DATA/$LIB.SingleReads.fa"
25
- else
26
- echo "$0: Error: No task selected, neither USECOUPLED nor USESINGLE set to yes." >&2
27
- exit 1;
28
- fi
29
- let SIZE=30*$(ls -l "$INPUT" | awk '{print $5}')/1024/1024/1024;
30
- let RAM=\(3+$SIZE\)*$RAMMULT;
31
- VARS="LIB=$LIB,PDIR=$PDIR,BIN454=$BIN454,KVELVET=$K_VELVET,KSOAP=$K_SOAP"
32
- # Launch Newbler
33
- NAME="Newbler_${LIB}"
34
- if [[ "$QUEUE" != "" ]] ; then
35
- qsub "$PDIR/newbler.pbs" -v "$VARS" -d "$SCRATCH" -N "$NAME" -l nodes=1:ppn=$PPN -l mem=${RAM}g -l "walltime=$WTIME" -q "$QUEUE"
36
- elif [[ $RAM -gt 150 ]] ; then
37
- qsub "$PDIR/newbler.pbs" -v "$VARS" -d "$SCRATCH" -N "$NAME" -l nodes=1:ppn=$PPN -l mem=${RAM}g -l walltime=360:00:00 -q biohimem-6
38
- elif [[ $SIZE -lt 4 ]] ; then
39
- qsub "$PDIR/newbler.pbs" -v "$VARS" -d "$SCRATCH" -N "$NAME" -l nodes=1:ppn=$PPN -l mem=${RAM}g -l walltime=12:00:00 -q iw-shared-6
40
- else
41
- qsub "$PDIR/newbler.pbs" -v "$VARS" -d "$SCRATCH" -N "$NAME" -l nodes=1:ppn=$PPN -l mem=${RAM}g -l walltime=120:00:00 -q biocluster-6
42
- fi
43
- done
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-
@@ -1,50 +0,0 @@
1
- #!/bin/bash
2
-
3
- ##################### RUN
4
- # Check if it was sourced from RUNME-*.bash
5
- if [[ "$PDIR" == "" ]] ; then
6
- echo "$0: Error: This file is not stand-alone. Execute one of RUNME-*.bash as described in the README.txt file" >&2
7
- exit 1
8
- fi
9
-
10
- # Find the directory of the pipeline
11
- CWD=$(pwd)
12
- PDIR=$(dirname $(readlink -f $0));
13
-
14
- # Run it
15
- # Actually, this script doesn't run anything. It's meant to keep the
16
- # variables centralized.
17
-
18
- # Load config
19
- NAMES=$(ls $PDIR/CONFIG.*.bash | sed -e 's/.*CONFIG\./ * /' | sed -e 's/\.bash//');
20
- if [[ "$1" == "" ]] ; then
21
- if [[ "$HELP" == "" ]] ; then
22
- echo "
23
- Usage:
24
- $0 name
25
-
26
- name The name of the run. CONFIG.name.bash must exist.
27
-
28
- See $PDIR/README.txt for more information.
29
-
30
- Available names are:
31
- $NAMES
32
- " >&2
33
- else
34
- echo "$HELP
35
- Available names are:
36
- $NAMES
37
- " >&2
38
- fi
39
- exit 1
40
- fi
41
- if [[ ! -e "$PDIR/CONFIG.$1.bash" ]] ; then
42
- echo "$0: Error: Impossible to find $PDIR/CONFIG.$1.bash, available names are:
43
- $NAMES" >&2
44
- exit 1
45
- fi
46
- source "$PDIR/CONFIG.$1.bash"
47
-
48
- # Create the scratch directory
49
- if [[ ! -d $SCRATCH ]] ; then mkdir -p $SCRATCH ; fi;
50
-
@@ -1,37 +0,0 @@
1
-
2
- # @author: Luis M. Rodriguez-R
3
- # @update: Nov-29-2012
4
-
5
- kSelector <- function(file, lib){
6
- red <- rgb(0.6, 0, 0);
7
- d <- read.table(file, sep=" ", h=T, fill=T);
8
- d <- d[!is.na(d$N50) & !is.na(d$used), ];
9
- d$reads <- max(d$reads, na.rm=T)
10
- d <- d[order(d$K), ];
11
- rownames(d) <- 1:nrow(d);
12
- par(mar=c(5,4,4,5)+.1, cex=0.8);
13
- barplot(d$reads/1e6, names=d$K, col='white', ylab='Number of reads (in millions)', xlab='K',
14
- main=paste('Reads used and N50 by K-mers in the assembly of', lib));
15
- barplot(d$used/1e6, col='grey', add=T);
16
- par(new=T);
17
- plot(1:length(d$K)-0.5, d$N50, col=red, t='b', lty=2, pch=20, cex=1, lwd=1.5,
18
- xlim=c(0, length(d$K)), xaxt='n', yaxt='n', xlab='', ylab='');
19
- axis(4, col.axis=red);
20
- mtext('N50 (bp)', side=4, line=3, col=red);
21
- # Suggest best k-mers
22
- if(nrow(d) >= 3){
23
- x = data.frame(K=d$K, l=(d$N50 - mean(d$N50))/sd(d$N50), u=(d$used - mean(d$used))/sd(d$used));
24
- rownames(x) <- rownames(d)
25
- d <- cbind(d, sel=FALSE);
26
- k_s = c();
27
- for(l_star in c(2, 1/2, 1)){
28
- k_s_i = x$K[which.max(l_star*x$l + x$u)];
29
- k_s <- c(k_s, k_s_i);
30
- x <- x[x$K!=k_s_i, ];
31
- d$sel[d$K==k_s_i] <- TRUE;
32
- }
33
- abline(v=as.numeric(rownames(d)[d$sel])-0.5, col='darkgreen', lty=6);
34
- }
35
- return(d);
36
- }
37
-
@@ -1,68 +0,0 @@
1
- #!/bin/bash
2
- #PBS -l nodes=1:ppn=1
3
- #PBS -k oe
4
-
5
- # Some defaults for the parameters
6
- BIN454=${BIN454:-"$HOME/454/bin"};
7
-
8
- # Check mandatory variables
9
- if [[ "$LIB" == "" ]]; then
10
- echo "Error: LIB is mandatory" >&2
11
- exit 1;
12
- fi
13
- if [[ "$PDIR" == "" ]]; then
14
- echo "Error: PDIR is mandatory" >&2
15
- exit 1;
16
- fi
17
- if [[ "$KVELVET$KSOAP" == "" ]]; then
18
- echo "Error: KVELVET and/or KSOAP are mandatory" >&2
19
- exit 1;
20
- fi
21
-
22
- # Prepare input
23
- KVELVET=$(echo $KVELVET | sed -e 's/:/ /g')
24
- KSOAP=$(echo $KSOAP | sed -e 's/:/ /g')
25
- NP=$(cat "$PBS_NODEFILE" | wc -l)
26
- CWD=$(pwd)
27
- DIR="$CWD/$LIB.newbler"
28
- LOG="$DIR.log"
29
- module load perl/5.14.4
30
- export PATH=$PATH:$BIN454
31
-
32
- # Create project
33
- echo new > $DIR.proc
34
- nohup newAssembly $DIR > $LOG
35
-
36
- # Prepare Velvet
37
- if [[ "$KVELVET" != "" ]] ; then
38
- echo pre-velvet > $DIR.proc
39
- rm $LIB.velvet.tmp1 &>/dev/null
40
- for K in $KVELVET ; do
41
- perl "$PDIR/FastA.filterN.pl" "$LIB.velvet_$K/contigs.fa" >> $LIB.velvet.tmp1
42
- done
43
- perl "$PDIR/newbler_preparator.pl" $LIB.velvet.tmp1 $LIB.velvet.tmp2
44
- cd $DIR
45
- nohup addRun ../$LIB.velvet.tmp2 >> $LOG
46
- cd $CWD
47
- fi ;
48
-
49
- # Prepare SOAP
50
- if [[ "$KSOAP" != "" ]] ; then
51
- echo pre-soap > $DIR.proc
52
- rm $LIB.soap.tmp1 &>/dev/null
53
- for K in $KSOAP ; do
54
- cat "$LIB.soap_$K/O.contig" >> $LIB.soap.tmp1
55
- done
56
- perl "$PDIR/newbler_preparator.pl" $LIB.soap.tmp1 $LIB.soap.tmp2
57
- cd $DIR
58
- nohup addRun ../$LIB.soap.tmp2 >> $LOG
59
- cd $CWD
60
- fi ;
61
-
62
- # Run
63
- cd $DIR
64
- echo newbler > $DIR.proc
65
- nohup runProject -cpu $NP >> $LOG
66
- cd $CWD
67
- echo done > $DIR.proc
68
-
@@ -1,49 +0,0 @@
1
- #!/usr/bin/perl
2
-
3
-
4
- my ($in, $out) = @ARGV;
5
- ($in and $out) or die "
6
- Usage: $0 input.fa output.fa
7
- ";
8
-
9
- open IN, "<", $in or die "Cannot read file: $in: $!\n";
10
- open OUT,">", $out or die "Cannot create file: $out: $!\n";
11
-
12
- %reads=();
13
- @reads=();
14
- while(<IN>){
15
- chomp;
16
- if(/^\>/){
17
- $tag=$_;
18
- $reads{$tag}='';
19
- push(@reads,$tag);
20
- }else{
21
- $reads{$tag}.=$_;
22
- }
23
- }
24
- close(IN);
25
-
26
- for(0..$#reads){
27
- $tag=$reads[$_];
28
- $read=$reads{$tag};
29
- $l=length $read;
30
- if($l<100){
31
- next;
32
- }else{
33
- if($l<1500){
34
- print OUT "$tag\n$read\n";
35
- }else{
36
- $r=int($l/1500)+1;
37
- $start=0;
38
- $i=1;
39
- while($start<$l-100){
40
- $tag_new=$tag.':r'.$i;
41
- $i++;
42
- $read_new=substr($read,$start,1500);
43
- $start+=200;
44
- print OUT "$tag_new\n$read_new\n";
45
- }
46
- }
47
- }
48
- }
49
- close(OUT);
@@ -1,80 +0,0 @@
1
- #!/bin/bash
2
- #PBS -k oe
3
-
4
- # Some defaults for the parameters
5
- INSLEN=${INSLEN:-300};
6
- USECOUPLED=${USECOUPLED:-yes}
7
- USESINGLE=${USESINGLE:-no}
8
- CLEANUP=${CLEANUP:-yes}
9
-
10
- # Check mandatory variables
11
- if [[ "$LIB" == "" ]]; then
12
- echo "Error: LIB is mandatory" >&2
13
- exit 1;
14
- fi
15
- if [[ "$PDIR" == "" ]]; then
16
- echo "Error: PDIR is mandatory" >&2
17
- exit 1;
18
- fi
19
- if [[ "$DATA" == "" ]]; then
20
- echo "Error: DATA is mandatory" >&2
21
- exit 1;
22
- fi
23
-
24
- # Prepare input
25
- module load perl/5.14.4
26
- KMER=$PBS_ARRAYID
27
- DIR="$LIB.soap_$KMER"
28
- if [[ "$USECOUPLED" == "yes" ]]; then
29
- MAXRDLEN=${MAXRDLEN:-$(perl $PDIR/FastA.length.pl "$DATA/$LIB.CoupledReads.fa" | head -n 200000 | awk '{if($2>MAX) MAX=$2} END{print MAX}')}
30
- elif [[ "$USESINGLE" == "yes" ]]; then
31
- MAXRDLEN=${MAXRDLEN:-$(perl $PDIR/FastA.length.pl "$DATA/$LIB.SingleReads.fa" | head -n 200000 | awk '{if($2>MAX) MAX=$2} END{print MAX}')}
32
- else
33
- echo "$0: Error: Nothing to do, neither USECOUPLED nor USESINGLE set to yes." >&2
34
- exit 2
35
- fi
36
- NP=$(cat "$PBS_NODEFILE" | wc -l)
37
-
38
- # Config
39
- module load SOAP/denovo2/r240
40
- echo config > $DIR.proc
41
- echo "max_rd_len=$MAXRDLEN
42
- [LIB]
43
- reverse_seq=0
44
- asm_flag=3
45
- rank=1" > $DIR.config
46
- if [[ "$USECOUPLED" == "yes" ]]; then
47
- echo "avg_ins=$INSLEN
48
- p=$DATA/$LIB.CoupledReads.fa" >> $DIR.config
49
- fi
50
- if [[ "$USESINGLE" == "yes" ]]; then
51
- echo "f=$DATA/$LIB.SingleReads.fa" >> $DIR.config
52
- fi
53
-
54
-
55
- # Run
56
- echo pre > $DIR.proc
57
- if [[ -d $DIR ]] ; then rm -R $DIR ; fi
58
- mkdir $DIR
59
- echo soap > $DIR.proc
60
- SOAPdenovo-63mer all -s $DIR.config -p $NP -K $KMER -o $DIR/O &> $DIR.log
61
- if [[ -d $DIR ]] ; then
62
- if [[ -s $DIR/O.contig ]] ; then
63
- if [[ "$CLEANUP" != "no" ]] ; then
64
- echo cleanup > $DIR.proc
65
- rm $DIR/*edge
66
- rm $DIR/*vertex
67
- rm $DIR/*Arc*
68
- rm $DIR/*Graph*
69
- rm $DIR/*readInGap*
70
- fi
71
- echo done > $DIR.proc
72
- else
73
- echo "$0: Error: File $DIR/O.contig doesn't exist, something went wrong" >&2
74
- exit 1
75
- fi
76
- else
77
- echo "$0: Error: Directory $DIR doesn't exist, something went wrong" >&2
78
- exit 1
79
- fi
80
-
@@ -1,57 +0,0 @@
1
- #!/bin/bash
2
- #PBS -q iw-shared-6
3
- #PBS -l nodes=1:ppn=1
4
- #PBS -l mem=1gb
5
- #PBS -l walltime=3:00:00
6
- #PBS -k oe
7
-
8
- # Check mandatory variables
9
- if [[ "$LIB" == "" ]]; then
10
- echo "Error: LIB is mandatory" >&2
11
- exit 1;
12
- fi
13
- if [[ "$PDIR" == "" ]]; then
14
- echo "Error: PDIR is mandatory" >&2
15
- exit 1;
16
- fi
17
-
18
- # Run
19
- module load perl/5.14.4
20
- echo "K N50 used reads " > $LIB.velvet.n50
21
- echo "K N50 used reads " > $LIB.soap.n50
22
- for ID in $(seq 10 31); do
23
- let KMER=$ID*2+1
24
- DIRV="$LIB.velvet_$KMER"
25
- DIRS="$LIB.soap_$KMER"
26
- echo $KMER > $LIB.velvet.n50.$KMER
27
- echo $KMER > $LIB.soap.n50.$KMER
28
- # N50 (>=500)
29
- perl "$PDIR/FastA.N50.pl" "$DIRV/contigs.fa" 500 | grep '^N50' | sed -e 's/.*: //' >> $LIB.velvet.n50.$KMER
30
- perl "$PDIR/FastA.N50.pl" "$DIRS/O.contig" 500 | grep '^N50' | sed -e 's/.*: //' >> $LIB.soap.n50.$KMER
31
- # Used and Total reads
32
- tail -n 1 $DIRV/Log | sed -e 's/.* using \\([0-9]*\\)\\/\\([0-9]*\\) reads.*/\\1\\n\\2/' >> $LIB.velvet.n50.$KMER
33
- if [ -e "$DIRS/O.readOnContig" ] ; then
34
- cat "$DIRS/O.readOnContig" | grep -vc '^read' >> $LIB.soap.n50.$KMER
35
- elif [ -e "$DIRS/O.readOnContig.gz" ] ; then
36
- zcat "$DIRS/O.readOnContig.gz" | grep -vc '^read' >> $LIB.soap.n50.$KMER
37
- else
38
- echo 0 >> $LIB.soap.n50.$KMER
39
- fi
40
- head -n 1 $DIRS/O.peGrads | awk '{print $3}' >> $LIB.soap.n50.$KMER
41
- # Join
42
- (cat $LIB.velvet.n50.$KMER | tr "\\n" " "; echo) >> $LIB.velvet.n50
43
- rm $LIB.velvet.n50.$KMER
44
- (cat $LIB.soap.n50.$KMER | tr "\\n" " "; echo) >> $LIB.soap.n50
45
- rm $LIB.soap.n50.$KMER
46
- done
47
-
48
- # Create plot
49
- module load R/3.1.2
50
- echo "
51
- source('$PDIR/kSelector.R');
52
- pdf('$LIB.n50.pdf', 13, 7);
53
- kSelector('$LIB.velvet.n50', '$LIB (Velvet)');
54
- kSelector('$LIB.soap.n50', '$LIB (SOAP)');
55
- dev.off();
56
- " | R --vanilla -q
57
-
@@ -1,63 +0,0 @@
1
- #!/bin/bash
2
- #PBS -l nodes=1:ppn=1
3
- #PBS -k oe
4
-
5
- # Some defaults for the parameters
6
- FORMAT=${FORMAT:-fasta};
7
- INSLEN=${INSLEN:-300};
8
- USECOUPLED=${USECOUPLED:-yes};
9
- USESINGLE=${USESINGLE:-no};
10
- CLEANUP=${CLEANUP:-yes}
11
-
12
- # Check mandatory variables
13
- if [[ "$LIB" == "" ]]; then
14
- echo "Error: LIB is mandatory" >&2
15
- exit 1;
16
- fi
17
- if [[ "$PDIR" == "" ]]; then
18
- echo "Error: PDIR is mandatory" >&2
19
- exit 1;
20
- fi
21
- if [[ "$DATA" == "" ]]; then
22
- echo "Error: DATA is mandatory" >&2
23
- exit 1;
24
- fi
25
-
26
- # Prepare input
27
- KMER=$PBS_ARRAYID
28
- CWD=$(pwd)
29
- DIR="$CWD/$LIB.velvet_$KMER"
30
-
31
- # Run
32
- module load velvet/1.2.10
33
- echo velveth > $DIR.proc
34
- CMD="velveth_101_omp $DIR $KMER -$FORMAT"
35
- if [[ "$USECOUPLED" == "yes" ]]; then
36
- CMD="$CMD -shortPaired $DATA/$LIB.CoupledReads.fa"
37
- fi
38
- if [[ "$USESINGLE" == "yes" ]]; then
39
- CMD="$CMD -short $DATA/$LIB.SingleReads.fa"
40
- fi
41
- if [[ "$VELVETH_EXTRA" != "" ]]; then
42
- CMD="$CMD $VELVETH_EXTRA"
43
- fi
44
- $CMD &> $DIR.hlog
45
- echo velvetg > $DIR.proc
46
- velvetg_101_omp "$DIR" -exp_cov auto -cov_cutoff auto -ins_length "$INSLEN" $VELVETG_EXTRA &> $DIR.glog
47
- if [[ -d $DIR ]] ; then
48
- if [[ -s $DIR/contigs.fa ]] ; then
49
- if [[ "$CLEANUP" != "no" ]] ; then
50
- echo cleanup > $DIR.proc
51
- rm $DIR/Sequences
52
- rm $DIR/Roadmaps
53
- rm $DIR/*Graph*
54
- fi
55
- echo done > $DIR.proc
56
- else
57
- echo "$0: Error: File $DIR/contigs.fa doesn't exist, something went wrong" >&2
58
- exit 1
59
- fi
60
- else
61
- echo "$0: Error: Directory $DIR doesn't exist, something went wrong" >&2
62
- exit 1
63
- fi
@@ -1,38 +0,0 @@
1
- # blast.pbs pipeline
2
- # Step 01 : Initialize input files
3
-
4
- # 00. Read configuration
5
- cd $SCRATCH ;
6
- TASK="dry" ;
7
- source "$PDIR/RUNME.bash" ;
8
- echo "$PBS_JOBID" > "$SCRATCH/success/01.00" ;
9
-
10
- if [[ ! -e "$SCRATCH/success/01.01" ]] ; then
11
- # 01. BEGIN
12
- REGISTER_JOB "01" "01" "Custom BEGIN function" \
13
- && BEGIN \
14
- || exit 1 ;
15
- touch "$SCRATCH/success/01.01" ;
16
- fi
17
-
18
- if [[ ! -e "$SCRATCH/success/01.02" ]] ; then
19
- # 02. Split
20
- [[ -d "$SCRATCH/tmp/split" ]] && rm -R "$SCRATCH/tmp/split" ;
21
- REGISTER_JOB "01" "02" "Splitting query files" \
22
- && mkdir "$SCRATCH/tmp/split" \
23
- && perl "$PDIR/FastA.split.pl" "$INPUT" "$SCRATCH/tmp/split/$PROJ" "$MAX_JOBS" \
24
- || exit 1 ;
25
- touch "$SCRATCH/success/01.02" ;
26
- fi ;
27
-
28
- if [[ ! -e "$SCRATCH/success/01.03" ]] ; then
29
- # 03. Finalize
30
- REGISTER_JOB "01" "03" "Finalizing input preparation" \
31
- && mv "$SCRATCH/tmp/split" "$SCRATCH/tmp/in" \
32
- || exit 1 ;
33
- touch "$SCRATCH/success/01.03" ;
34
- fi ;
35
-
36
- [[ -d "$SCRATCH/tmp/out" ]] || ( mkdir "$SCRATCH/tmp/out" || exit 1 ) ;
37
- JOB_DONE "01" ;
38
-
@@ -1,73 +0,0 @@
1
- # blast.pbs pipeline
2
- # Step 02 : Run BLAST
3
-
4
- # Read configuration
5
- cd $SCRATCH ;
6
- TASK="dry" ;
7
- source "$PDIR/RUNME.bash" ;
8
-
9
- # 00. Initial vars
10
- ID_N=$PBS_ARRAYID
11
- [[ "$ID_N" == "" ]] && exit 1 ;
12
- [[ -e "$SCRATCH/success/02.$ID_N" ]] && exit 0 ;
13
- IN="$SCRATCH/tmp/in/$PROJ.$ID_N.fa" ;
14
- OUT="$SCRATCH/tmp/out/$PROJ.blast.$ID_N" ;
15
- FINAL_OUT="$SCRATCH/results/$PROJ.$ID_N.blast" ;
16
- if [[ -e "$SCRATCH/success/02.$ID_N.00" ]] ; then
17
- pre_job=$(cat "$SCRATCH/success/02.$ID_N.00") ;
18
- state=$(qstat -f "$pre_job" 2>/dev/null | grep job_state | sed -e 's/.*= //')
19
- if [[ "$state" == "R" ]] ; then
20
- echo "Warning: This task is already being executed by $pre_job. Aborting." >&2 ;
21
- exit 0 ;
22
- elif [[ "$state" == "" ]] ; then
23
- echo "Warning: This task was initialized by $pre_job, but it's currently not running. Superseding." >&2 ;
24
- fi ;
25
- fi
26
- echo "$PBS_JOBID" > "$SCRATCH/success/02.$ID_N.00" ;
27
-
28
- # 01. Before BLAST
29
- if [[ ! -e "$SCRATCH/success/02.$ID_N.01" ]] ; then
30
- BEFORE_BLAST "$IN" "$OUT" || exit 1 ;
31
- touch "$SCRATCH/success/02.$ID_N.01" ;
32
- fi ;
33
-
34
- # 02. Run BLAST
35
- if [[ ! -e "$SCRATCH/success/02.$ID_N.02" ]] ; then
36
- # Recover previous runs, if any
37
- if [[ -s "$OUT" ]] ; then
38
- perl "$PDIR/BlastTab.recover_job.pl" "$IN" "$OUT" \
39
- || exit 1 ;
40
- fi ;
41
- # Run BLAST
42
- RUN_BLAST "$IN" "$OUT" \
43
- && mv "$OUT" "$OUT-z" \
44
- || exit 1 ;
45
- touch "$SCRATCH/success/02.$ID_N.02" ;
46
- fi ;
47
-
48
- # 03. Collect BLAST parts
49
- if [[ ! -e "$SCRATCH/success/02.$ID_N.03" ]] ; then
50
- if [[ -e "$OUT" ]] ; then
51
- echo "Warning: The file $OUT pre-exists, but the BLAST collection was incomplete." >&2 ;
52
- echo " I'm assuming that it corresponds to the first part of the result, but you should check manually." >&2 ;
53
- echo " The last lines are:" >&2 ;
54
- tail -n 3 "$OUT" >&2 ;
55
- else
56
- touch "$OUT" || exit 1 ;
57
- fi ;
58
- for i in $(ls $OUT-*) ; do
59
- cat "$i" >> "$OUT" ;
60
- rm "$i" || exit 1 ;
61
- done ;
62
- mv "$OUT" "$FINAL_OUT"
63
- touch "$SCRATCH/success/02.$ID_N.03" ;
64
- fi ;
65
-
66
- # 04. After BLAST
67
- if [[ ! -e "$SCRATCH/success/02.$ID_N.04" ]] ; then
68
- AFTER_BLAST "$IN" "$FINAL_OUT" || exit 1 ;
69
- touch "$SCRATCH/success/02.$ID_N.04" ;
70
- fi ;
71
-
72
- touch "$SCRATCH/success/02.$ID_N" ;
73
-
@@ -1,21 +0,0 @@
1
- # blast.pbs pipeline
2
- # Step 03 : Finalize
3
-
4
- # Read configuration
5
- cd $SCRATCH ;
6
- TASK="dry" ;
7
- source "$PDIR/RUNME.bash" ;
8
- PREFIX="$SCRATCH/results/$PROJ" ;
9
- OUT="$SCRATCH/$PROJ.blast" ;
10
- echo "$PBS_JOBID" > "$SCRATCH/success/02.00" ;
11
-
12
- # 01. END
13
- if [[ ! -e "$SCRATCH/success/03.01" ]] ; then
14
- REGISTER_JOB "03" "01" "Custom END function" \
15
- && END "$PREFIX" "$OUT" \
16
- || exit 1 ;
17
- touch "$SCRATCH/success/03.01" ;
18
- fi ;
19
-
20
- JOB_DONE "03" ;
21
-
@@ -1,72 +0,0 @@
1
- #!/usr/bin/perl
2
-
3
- use warnings;
4
- use strict;
5
- use File::Copy;
6
-
7
- my($fasta, $blast) = @ARGV;
8
-
9
- ($fasta and $blast) or die "
10
- .USAGE:
11
- $0 query.fa blast.txt
12
-
13
- query.fa Query sequences in FastA format.
14
- blast.txt Incomplete BLAST output in tabular format.
15
-
16
- ";
17
-
18
- print "Fixing $blast:\n";
19
- my $blast_res;
20
- for(my $i=0; 1; $i++){
21
- $blast_res = "$blast-$i";
22
- last unless -e $blast_res;
23
- }
24
- open BLAST, "<", $blast or die "Cannot read the file: $blast: $!\n";
25
- open TMP, ">", "$blast-tmp" or die "Cannot create the file: $blast-tmp: $!\n";
26
- my $last="";
27
- my $last_id="";
28
- my $before = "";
29
- while(my $ln=<BLAST>){
30
- chomp $ln;
31
- last unless $ln =~ m/(.+?)\t/;
32
- my $id = $1;
33
- if($id eq $last_id){
34
- $last.= $ln."\n";
35
- }else{
36
- print TMP $last if $last;
37
- $before = $last_id;
38
- $last = $ln."\n";
39
- $last_id = $id;
40
- }
41
- }
42
- close BLAST;
43
- close TMP;
44
-
45
- move "$blast-tmp", $blast_res or die "Cannot move file $blast-tmp into $blast_res: $!\n";
46
- unlink $blast or die "Cannot delete file: $blast: $!\n";
47
-
48
- unless($before eq ""){
49
- print "[$before] ";
50
- $before = ">$before";
51
-
52
- open FASTA, "<", $fasta or die "Cannot read file: $fasta: $!\n";
53
- open TMP, ">", "$fasta-tmp" or die "Cannot create file: $fasta-tmp: $!\n";
54
- my $print = 0;
55
- my $at = 0;
56
- my $i = 0;
57
- while(my $ln=<FASTA>){
58
- $i++;
59
- $print = 1 if $at and $ln =~ /^>/;
60
- print TMP $ln if $print;
61
- $ln =~ s/\s+.*//;
62
- chomp $ln;
63
- $at = $i if $ln eq $before;
64
- }
65
- close TMP;
66
- close FASTA;
67
- printf 'recovered at %.2f%% (%d/%d).'."\n", 100*$at/$i, $at, $i if $i;
68
-
69
- move $fasta, "$fasta.old" or die "Cannot move file $fasta into $fasta.old: $!\n";
70
- move "$fasta-tmp", $fasta or die "Cannot move file $fasta-tmp into $fasta: $!\n";
71
- }
72
-