miga-base 1.2.15.2 → 1.2.15.3

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Files changed (304) hide show
  1. checksums.yaml +4 -4
  2. data/lib/miga/cli/action/download/gtdb.rb +4 -1
  3. data/lib/miga/cli/action/gtdb_get.rb +4 -0
  4. data/lib/miga/remote_dataset/download.rb +3 -2
  5. data/lib/miga/remote_dataset.rb +25 -7
  6. data/lib/miga/taxonomy.rb +6 -0
  7. data/lib/miga/version.rb +2 -2
  8. metadata +6 -302
  9. data/utils/FastAAI/00.Libraries/01.SCG_HMMs/Archaea_SCG.hmm +0 -41964
  10. data/utils/FastAAI/00.Libraries/01.SCG_HMMs/Bacteria_SCG.hmm +0 -32439
  11. data/utils/FastAAI/00.Libraries/01.SCG_HMMs/Complete_SCG_DB.hmm +0 -62056
  12. data/utils/FastAAI/FastAAI +0 -3659
  13. data/utils/FastAAI/FastAAI-legacy/FastAAI +0 -1336
  14. data/utils/FastAAI/FastAAI-legacy/kAAI_v1.0_virus.py +0 -1296
  15. data/utils/FastAAI/README.md +0 -84
  16. data/utils/enveomics/Docs/recplot2.md +0 -244
  17. data/utils/enveomics/Examples/aai-matrix.bash +0 -66
  18. data/utils/enveomics/Examples/ani-matrix.bash +0 -66
  19. data/utils/enveomics/Examples/essential-phylogeny.bash +0 -105
  20. data/utils/enveomics/Examples/unus-genome-phylogeny.bash +0 -100
  21. data/utils/enveomics/LICENSE.txt +0 -73
  22. data/utils/enveomics/Makefile +0 -52
  23. data/utils/enveomics/Manifest/Tasks/aasubs.json +0 -103
  24. data/utils/enveomics/Manifest/Tasks/blasttab.json +0 -790
  25. data/utils/enveomics/Manifest/Tasks/distances.json +0 -161
  26. data/utils/enveomics/Manifest/Tasks/fasta.json +0 -802
  27. data/utils/enveomics/Manifest/Tasks/fastq.json +0 -291
  28. data/utils/enveomics/Manifest/Tasks/graphics.json +0 -126
  29. data/utils/enveomics/Manifest/Tasks/mapping.json +0 -137
  30. data/utils/enveomics/Manifest/Tasks/ogs.json +0 -382
  31. data/utils/enveomics/Manifest/Tasks/other.json +0 -906
  32. data/utils/enveomics/Manifest/Tasks/remote.json +0 -355
  33. data/utils/enveomics/Manifest/Tasks/sequence-identity.json +0 -650
  34. data/utils/enveomics/Manifest/Tasks/tables.json +0 -308
  35. data/utils/enveomics/Manifest/Tasks/trees.json +0 -68
  36. data/utils/enveomics/Manifest/Tasks/variants.json +0 -111
  37. data/utils/enveomics/Manifest/categories.json +0 -165
  38. data/utils/enveomics/Manifest/examples.json +0 -162
  39. data/utils/enveomics/Manifest/tasks.json +0 -4
  40. data/utils/enveomics/Pipelines/assembly.pbs/CONFIG.mock.bash +0 -69
  41. data/utils/enveomics/Pipelines/assembly.pbs/FastA.N50.pl +0 -1
  42. data/utils/enveomics/Pipelines/assembly.pbs/FastA.filterN.pl +0 -1
  43. data/utils/enveomics/Pipelines/assembly.pbs/FastA.length.pl +0 -1
  44. data/utils/enveomics/Pipelines/assembly.pbs/README.md +0 -189
  45. data/utils/enveomics/Pipelines/assembly.pbs/RUNME-2.bash +0 -112
  46. data/utils/enveomics/Pipelines/assembly.pbs/RUNME-3.bash +0 -23
  47. data/utils/enveomics/Pipelines/assembly.pbs/RUNME-4.bash +0 -44
  48. data/utils/enveomics/Pipelines/assembly.pbs/RUNME.bash +0 -50
  49. data/utils/enveomics/Pipelines/assembly.pbs/kSelector.R +0 -37
  50. data/utils/enveomics/Pipelines/assembly.pbs/newbler.pbs +0 -68
  51. data/utils/enveomics/Pipelines/assembly.pbs/newbler_preparator.pl +0 -49
  52. data/utils/enveomics/Pipelines/assembly.pbs/soap.pbs +0 -80
  53. data/utils/enveomics/Pipelines/assembly.pbs/stats.pbs +0 -57
  54. data/utils/enveomics/Pipelines/assembly.pbs/velvet.pbs +0 -63
  55. data/utils/enveomics/Pipelines/blast.pbs/01.pbs.bash +0 -38
  56. data/utils/enveomics/Pipelines/blast.pbs/02.pbs.bash +0 -73
  57. data/utils/enveomics/Pipelines/blast.pbs/03.pbs.bash +0 -21
  58. data/utils/enveomics/Pipelines/blast.pbs/BlastTab.recover_job.pl +0 -72
  59. data/utils/enveomics/Pipelines/blast.pbs/CONFIG.mock.bash +0 -98
  60. data/utils/enveomics/Pipelines/blast.pbs/FastA.split.pl +0 -1
  61. data/utils/enveomics/Pipelines/blast.pbs/README.md +0 -127
  62. data/utils/enveomics/Pipelines/blast.pbs/RUNME.bash +0 -109
  63. data/utils/enveomics/Pipelines/blast.pbs/TASK.check.bash +0 -128
  64. data/utils/enveomics/Pipelines/blast.pbs/TASK.dry.bash +0 -16
  65. data/utils/enveomics/Pipelines/blast.pbs/TASK.eo.bash +0 -22
  66. data/utils/enveomics/Pipelines/blast.pbs/TASK.pause.bash +0 -26
  67. data/utils/enveomics/Pipelines/blast.pbs/TASK.run.bash +0 -89
  68. data/utils/enveomics/Pipelines/blast.pbs/sentinel.pbs.bash +0 -29
  69. data/utils/enveomics/Pipelines/idba.pbs/README.md +0 -49
  70. data/utils/enveomics/Pipelines/idba.pbs/RUNME.bash +0 -95
  71. data/utils/enveomics/Pipelines/idba.pbs/run.pbs +0 -56
  72. data/utils/enveomics/Pipelines/trim.pbs/README.md +0 -54
  73. data/utils/enveomics/Pipelines/trim.pbs/RUNME.bash +0 -70
  74. data/utils/enveomics/Pipelines/trim.pbs/run.pbs +0 -130
  75. data/utils/enveomics/README.md +0 -42
  76. data/utils/enveomics/Scripts/AAsubs.log2ratio.rb +0 -171
  77. data/utils/enveomics/Scripts/Aln.cat.rb +0 -221
  78. data/utils/enveomics/Scripts/Aln.convert.pl +0 -35
  79. data/utils/enveomics/Scripts/AlphaDiversity.pl +0 -152
  80. data/utils/enveomics/Scripts/BedGraph.tad.rb +0 -93
  81. data/utils/enveomics/Scripts/BedGraph.window.rb +0 -71
  82. data/utils/enveomics/Scripts/BlastPairwise.AAsubs.pl +0 -102
  83. data/utils/enveomics/Scripts/BlastTab.addlen.rb +0 -63
  84. data/utils/enveomics/Scripts/BlastTab.advance.bash +0 -48
  85. data/utils/enveomics/Scripts/BlastTab.best_hit_sorted.pl +0 -55
  86. data/utils/enveomics/Scripts/BlastTab.catsbj.pl +0 -104
  87. data/utils/enveomics/Scripts/BlastTab.cogCat.rb +0 -76
  88. data/utils/enveomics/Scripts/BlastTab.filter.pl +0 -47
  89. data/utils/enveomics/Scripts/BlastTab.kegg_pep2path_rest.pl +0 -194
  90. data/utils/enveomics/Scripts/BlastTab.metaxaPrep.pl +0 -104
  91. data/utils/enveomics/Scripts/BlastTab.pairedHits.rb +0 -157
  92. data/utils/enveomics/Scripts/BlastTab.recplot2.R +0 -48
  93. data/utils/enveomics/Scripts/BlastTab.seqdepth.pl +0 -86
  94. data/utils/enveomics/Scripts/BlastTab.seqdepth_ZIP.pl +0 -119
  95. data/utils/enveomics/Scripts/BlastTab.seqdepth_nomedian.pl +0 -86
  96. data/utils/enveomics/Scripts/BlastTab.subsample.pl +0 -47
  97. data/utils/enveomics/Scripts/BlastTab.sumPerHit.pl +0 -114
  98. data/utils/enveomics/Scripts/BlastTab.taxid2taxrank.pl +0 -90
  99. data/utils/enveomics/Scripts/BlastTab.topHits_sorted.rb +0 -123
  100. data/utils/enveomics/Scripts/Chao1.pl +0 -97
  101. data/utils/enveomics/Scripts/CharTable.classify.rb +0 -234
  102. data/utils/enveomics/Scripts/EBIseq2tax.rb +0 -83
  103. data/utils/enveomics/Scripts/FastA.N50.pl +0 -60
  104. data/utils/enveomics/Scripts/FastA.extract.rb +0 -152
  105. data/utils/enveomics/Scripts/FastA.filter.pl +0 -52
  106. data/utils/enveomics/Scripts/FastA.filterLen.pl +0 -28
  107. data/utils/enveomics/Scripts/FastA.filterN.pl +0 -60
  108. data/utils/enveomics/Scripts/FastA.fragment.rb +0 -100
  109. data/utils/enveomics/Scripts/FastA.gc.pl +0 -42
  110. data/utils/enveomics/Scripts/FastA.interpose.pl +0 -93
  111. data/utils/enveomics/Scripts/FastA.length.pl +0 -38
  112. data/utils/enveomics/Scripts/FastA.mask.rb +0 -89
  113. data/utils/enveomics/Scripts/FastA.per_file.pl +0 -36
  114. data/utils/enveomics/Scripts/FastA.qlen.pl +0 -57
  115. data/utils/enveomics/Scripts/FastA.rename.pl +0 -65
  116. data/utils/enveomics/Scripts/FastA.revcom.pl +0 -23
  117. data/utils/enveomics/Scripts/FastA.sample.rb +0 -98
  118. data/utils/enveomics/Scripts/FastA.slider.pl +0 -85
  119. data/utils/enveomics/Scripts/FastA.split.pl +0 -55
  120. data/utils/enveomics/Scripts/FastA.split.rb +0 -79
  121. data/utils/enveomics/Scripts/FastA.subsample.pl +0 -131
  122. data/utils/enveomics/Scripts/FastA.tag.rb +0 -65
  123. data/utils/enveomics/Scripts/FastA.toFastQ.rb +0 -69
  124. data/utils/enveomics/Scripts/FastA.wrap.rb +0 -48
  125. data/utils/enveomics/Scripts/FastQ.filter.pl +0 -54
  126. data/utils/enveomics/Scripts/FastQ.interpose.pl +0 -90
  127. data/utils/enveomics/Scripts/FastQ.maskQual.rb +0 -89
  128. data/utils/enveomics/Scripts/FastQ.offset.pl +0 -90
  129. data/utils/enveomics/Scripts/FastQ.split.pl +0 -53
  130. data/utils/enveomics/Scripts/FastQ.tag.rb +0 -70
  131. data/utils/enveomics/Scripts/FastQ.test-error.rb +0 -81
  132. data/utils/enveomics/Scripts/FastQ.toFastA.awk +0 -24
  133. data/utils/enveomics/Scripts/GFF.catsbj.pl +0 -127
  134. data/utils/enveomics/Scripts/GenBank.add_fields.rb +0 -84
  135. data/utils/enveomics/Scripts/HMM.essential.rb +0 -351
  136. data/utils/enveomics/Scripts/HMM.haai.rb +0 -168
  137. data/utils/enveomics/Scripts/HMMsearch.extractIds.rb +0 -83
  138. data/utils/enveomics/Scripts/JPlace.distances.rb +0 -88
  139. data/utils/enveomics/Scripts/JPlace.to_iToL.rb +0 -320
  140. data/utils/enveomics/Scripts/M5nr.getSequences.rb +0 -81
  141. data/utils/enveomics/Scripts/MeTaxa.distribution.pl +0 -198
  142. data/utils/enveomics/Scripts/MyTaxa.fragsByTax.pl +0 -35
  143. data/utils/enveomics/Scripts/MyTaxa.seq-taxrank.rb +0 -49
  144. data/utils/enveomics/Scripts/NCBIacc2tax.rb +0 -92
  145. data/utils/enveomics/Scripts/Newick.autoprune.R +0 -27
  146. data/utils/enveomics/Scripts/RAxML-EPA.to_iToL.pl +0 -228
  147. data/utils/enveomics/Scripts/RecPlot2.compareIdentities.R +0 -32
  148. data/utils/enveomics/Scripts/RefSeq.download.bash +0 -48
  149. data/utils/enveomics/Scripts/SRA.download.bash +0 -55
  150. data/utils/enveomics/Scripts/TRIBS.plot-test.R +0 -36
  151. data/utils/enveomics/Scripts/TRIBS.test.R +0 -39
  152. data/utils/enveomics/Scripts/Table.barplot.R +0 -31
  153. data/utils/enveomics/Scripts/Table.df2dist.R +0 -30
  154. data/utils/enveomics/Scripts/Table.filter.pl +0 -61
  155. data/utils/enveomics/Scripts/Table.merge.pl +0 -77
  156. data/utils/enveomics/Scripts/Table.prefScore.R +0 -60
  157. data/utils/enveomics/Scripts/Table.replace.rb +0 -69
  158. data/utils/enveomics/Scripts/Table.round.rb +0 -63
  159. data/utils/enveomics/Scripts/Table.split.pl +0 -57
  160. data/utils/enveomics/Scripts/Taxonomy.silva2ncbi.rb +0 -227
  161. data/utils/enveomics/Scripts/VCF.KaKs.rb +0 -147
  162. data/utils/enveomics/Scripts/VCF.SNPs.rb +0 -88
  163. data/utils/enveomics/Scripts/aai.rb +0 -421
  164. data/utils/enveomics/Scripts/ani.rb +0 -362
  165. data/utils/enveomics/Scripts/anir.rb +0 -137
  166. data/utils/enveomics/Scripts/clust.rand.rb +0 -102
  167. data/utils/enveomics/Scripts/gi2tax.rb +0 -103
  168. data/utils/enveomics/Scripts/in_silico_GA_GI.pl +0 -96
  169. data/utils/enveomics/Scripts/lib/data/dupont_2012_essential.hmm.gz +0 -0
  170. data/utils/enveomics/Scripts/lib/data/lee_2019_essential.hmm.gz +0 -0
  171. data/utils/enveomics/Scripts/lib/enveomics.R +0 -1
  172. data/utils/enveomics/Scripts/lib/enveomics_rb/anir.rb +0 -293
  173. data/utils/enveomics/Scripts/lib/enveomics_rb/bm_set.rb +0 -175
  174. data/utils/enveomics/Scripts/lib/enveomics_rb/enveomics.rb +0 -24
  175. data/utils/enveomics/Scripts/lib/enveomics_rb/errors.rb +0 -17
  176. data/utils/enveomics/Scripts/lib/enveomics_rb/gmm_em.rb +0 -30
  177. data/utils/enveomics/Scripts/lib/enveomics_rb/jplace.rb +0 -253
  178. data/utils/enveomics/Scripts/lib/enveomics_rb/match.rb +0 -88
  179. data/utils/enveomics/Scripts/lib/enveomics_rb/og.rb +0 -182
  180. data/utils/enveomics/Scripts/lib/enveomics_rb/rbm.rb +0 -49
  181. data/utils/enveomics/Scripts/lib/enveomics_rb/remote_data.rb +0 -74
  182. data/utils/enveomics/Scripts/lib/enveomics_rb/seq_range.rb +0 -237
  183. data/utils/enveomics/Scripts/lib/enveomics_rb/stats/rand.rb +0 -31
  184. data/utils/enveomics/Scripts/lib/enveomics_rb/stats/sample.rb +0 -152
  185. data/utils/enveomics/Scripts/lib/enveomics_rb/stats.rb +0 -3
  186. data/utils/enveomics/Scripts/lib/enveomics_rb/utils.rb +0 -74
  187. data/utils/enveomics/Scripts/lib/enveomics_rb/vcf.rb +0 -135
  188. data/utils/enveomics/Scripts/ogs.annotate.rb +0 -88
  189. data/utils/enveomics/Scripts/ogs.core-pan.rb +0 -160
  190. data/utils/enveomics/Scripts/ogs.extract.rb +0 -125
  191. data/utils/enveomics/Scripts/ogs.mcl.rb +0 -186
  192. data/utils/enveomics/Scripts/ogs.rb +0 -104
  193. data/utils/enveomics/Scripts/ogs.stats.rb +0 -131
  194. data/utils/enveomics/Scripts/rbm-legacy.rb +0 -172
  195. data/utils/enveomics/Scripts/rbm.rb +0 -108
  196. data/utils/enveomics/Scripts/sam.filter.rb +0 -148
  197. data/utils/enveomics/Tests/Makefile +0 -10
  198. data/utils/enveomics/Tests/Mgen_M2288.faa +0 -3189
  199. data/utils/enveomics/Tests/Mgen_M2288.fna +0 -8282
  200. data/utils/enveomics/Tests/Mgen_M2321.fna +0 -8288
  201. data/utils/enveomics/Tests/Nequ_Kin4M.faa +0 -2970
  202. data/utils/enveomics/Tests/Xanthomonas_oryzae-PilA.tribs.Rdata +0 -0
  203. data/utils/enveomics/Tests/Xanthomonas_oryzae-PilA.txt +0 -7
  204. data/utils/enveomics/Tests/Xanthomonas_oryzae.aai-mat.tsv +0 -17
  205. data/utils/enveomics/Tests/Xanthomonas_oryzae.aai.tsv +0 -137
  206. data/utils/enveomics/Tests/a_mg.cds-go.blast.tsv +0 -123
  207. data/utils/enveomics/Tests/a_mg.reads-cds.blast.tsv +0 -200
  208. data/utils/enveomics/Tests/a_mg.reads-cds.counts.tsv +0 -55
  209. data/utils/enveomics/Tests/alkB.nwk +0 -1
  210. data/utils/enveomics/Tests/anthrax-cansnp-data.tsv +0 -13
  211. data/utils/enveomics/Tests/anthrax-cansnp-key.tsv +0 -17
  212. data/utils/enveomics/Tests/hiv1.faa +0 -59
  213. data/utils/enveomics/Tests/hiv1.fna +0 -134
  214. data/utils/enveomics/Tests/hiv2.faa +0 -70
  215. data/utils/enveomics/Tests/hiv_mix-hiv1.blast.tsv +0 -233
  216. data/utils/enveomics/Tests/hiv_mix-hiv1.blast.tsv.lim +0 -1
  217. data/utils/enveomics/Tests/hiv_mix-hiv1.blast.tsv.rec +0 -233
  218. data/utils/enveomics/Tests/phyla_counts.tsv +0 -10
  219. data/utils/enveomics/Tests/primate_lentivirus.ogs +0 -11
  220. data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv1-hiv1.rbm +0 -9
  221. data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv1-hiv2.rbm +0 -8
  222. data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv1-siv.rbm +0 -6
  223. data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv2-hiv2.rbm +0 -9
  224. data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv2-siv.rbm +0 -6
  225. data/utils/enveomics/Tests/primate_lentivirus.rbm/siv-siv.rbm +0 -6
  226. data/utils/enveomics/build_enveomics_r.bash +0 -45
  227. data/utils/enveomics/enveomics.R/DESCRIPTION +0 -31
  228. data/utils/enveomics/enveomics.R/NAMESPACE +0 -39
  229. data/utils/enveomics/enveomics.R/R/autoprune.R +0 -155
  230. data/utils/enveomics/enveomics.R/R/barplot.R +0 -184
  231. data/utils/enveomics/enveomics.R/R/cliopts.R +0 -135
  232. data/utils/enveomics/enveomics.R/R/df2dist.R +0 -154
  233. data/utils/enveomics/enveomics.R/R/growthcurve.R +0 -331
  234. data/utils/enveomics/enveomics.R/R/prefscore.R +0 -79
  235. data/utils/enveomics/enveomics.R/R/recplot.R +0 -354
  236. data/utils/enveomics/enveomics.R/R/recplot2.R +0 -1631
  237. data/utils/enveomics/enveomics.R/R/tribs.R +0 -583
  238. data/utils/enveomics/enveomics.R/R/utils.R +0 -80
  239. data/utils/enveomics/enveomics.R/README.md +0 -81
  240. data/utils/enveomics/enveomics.R/data/growth.curves.rda +0 -0
  241. data/utils/enveomics/enveomics.R/data/phyla.counts.rda +0 -0
  242. data/utils/enveomics/enveomics.R/man/cash-enve.GrowthCurve-method.Rd +0 -16
  243. data/utils/enveomics/enveomics.R/man/cash-enve.RecPlot2-method.Rd +0 -16
  244. data/utils/enveomics/enveomics.R/man/cash-enve.RecPlot2.Peak-method.Rd +0 -16
  245. data/utils/enveomics/enveomics.R/man/enve.GrowthCurve-class.Rd +0 -25
  246. data/utils/enveomics/enveomics.R/man/enve.TRIBS-class.Rd +0 -46
  247. data/utils/enveomics/enveomics.R/man/enve.TRIBS.merge.Rd +0 -23
  248. data/utils/enveomics/enveomics.R/man/enve.TRIBStest-class.Rd +0 -47
  249. data/utils/enveomics/enveomics.R/man/enve.__prune.iter.Rd +0 -23
  250. data/utils/enveomics/enveomics.R/man/enve.__prune.reduce.Rd +0 -23
  251. data/utils/enveomics/enveomics.R/man/enve.__tribs.Rd +0 -40
  252. data/utils/enveomics/enveomics.R/man/enve.barplot.Rd +0 -103
  253. data/utils/enveomics/enveomics.R/man/enve.cliopts.Rd +0 -67
  254. data/utils/enveomics/enveomics.R/man/enve.col.alpha.Rd +0 -24
  255. data/utils/enveomics/enveomics.R/man/enve.col2alpha.Rd +0 -19
  256. data/utils/enveomics/enveomics.R/man/enve.df2dist.Rd +0 -45
  257. data/utils/enveomics/enveomics.R/man/enve.df2dist.group.Rd +0 -44
  258. data/utils/enveomics/enveomics.R/man/enve.df2dist.list.Rd +0 -47
  259. data/utils/enveomics/enveomics.R/man/enve.growthcurve.Rd +0 -75
  260. data/utils/enveomics/enveomics.R/man/enve.prefscore.Rd +0 -50
  261. data/utils/enveomics/enveomics.R/man/enve.prune.dist.Rd +0 -44
  262. data/utils/enveomics/enveomics.R/man/enve.recplot.Rd +0 -139
  263. data/utils/enveomics/enveomics.R/man/enve.recplot2-class.Rd +0 -45
  264. data/utils/enveomics/enveomics.R/man/enve.recplot2.ANIr.Rd +0 -24
  265. data/utils/enveomics/enveomics.R/man/enve.recplot2.Rd +0 -77
  266. data/utils/enveomics/enveomics.R/man/enve.recplot2.__counts.Rd +0 -25
  267. data/utils/enveomics/enveomics.R/man/enve.recplot2.__peakHist.Rd +0 -21
  268. data/utils/enveomics/enveomics.R/man/enve.recplot2.__whichClosestPeak.Rd +0 -19
  269. data/utils/enveomics/enveomics.R/man/enve.recplot2.changeCutoff.Rd +0 -19
  270. data/utils/enveomics/enveomics.R/man/enve.recplot2.compareIdentities.Rd +0 -47
  271. data/utils/enveomics/enveomics.R/man/enve.recplot2.coordinates.Rd +0 -29
  272. data/utils/enveomics/enveomics.R/man/enve.recplot2.corePeak.Rd +0 -18
  273. data/utils/enveomics/enveomics.R/man/enve.recplot2.extractWindows.Rd +0 -45
  274. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.Rd +0 -36
  275. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__em_e.Rd +0 -19
  276. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__em_m.Rd +0 -19
  277. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__emauto_one.Rd +0 -27
  278. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__mow_one.Rd +0 -52
  279. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__mower.Rd +0 -17
  280. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.em.Rd +0 -51
  281. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.emauto.Rd +0 -43
  282. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.mower.Rd +0 -82
  283. data/utils/enveomics/enveomics.R/man/enve.recplot2.peak-class.Rd +0 -59
  284. data/utils/enveomics/enveomics.R/man/enve.recplot2.seqdepth.Rd +0 -27
  285. data/utils/enveomics/enveomics.R/man/enve.recplot2.windowDepthThreshold.Rd +0 -36
  286. data/utils/enveomics/enveomics.R/man/enve.selvector.Rd +0 -23
  287. data/utils/enveomics/enveomics.R/man/enve.tribs.Rd +0 -68
  288. data/utils/enveomics/enveomics.R/man/enve.tribs.test.Rd +0 -28
  289. data/utils/enveomics/enveomics.R/man/enve.truncate.Rd +0 -27
  290. data/utils/enveomics/enveomics.R/man/growth.curves.Rd +0 -14
  291. data/utils/enveomics/enveomics.R/man/phyla.counts.Rd +0 -13
  292. data/utils/enveomics/enveomics.R/man/plot.enve.GrowthCurve.Rd +0 -78
  293. data/utils/enveomics/enveomics.R/man/plot.enve.TRIBS.Rd +0 -46
  294. data/utils/enveomics/enveomics.R/man/plot.enve.TRIBStest.Rd +0 -45
  295. data/utils/enveomics/enveomics.R/man/plot.enve.recplot2.Rd +0 -125
  296. data/utils/enveomics/enveomics.R/man/summary.enve.GrowthCurve.Rd +0 -19
  297. data/utils/enveomics/enveomics.R/man/summary.enve.TRIBS.Rd +0 -19
  298. data/utils/enveomics/enveomics.R/man/summary.enve.TRIBStest.Rd +0 -19
  299. data/utils/enveomics/globals.mk +0 -8
  300. data/utils/enveomics/manifest.json +0 -9
  301. data/utils/multitrim/Multitrim How-To.pdf +0 -0
  302. data/utils/multitrim/README.md +0 -67
  303. data/utils/multitrim/multitrim.py +0 -1555
  304. data/utils/multitrim/multitrim.yml +0 -13
@@ -1,127 +0,0 @@
1
- @author: Luis Miguel Rodriguez-R <lmrodriguezr at gmail dot com>
2
-
3
- @update: Feb-20-2014
4
-
5
- @license: artistic 2.0
6
-
7
- @status: auto
8
-
9
- @pbs: yes
10
-
11
- # IMPORTANT
12
-
13
- This pipeline was developed for the [PACE cluster](http://pace.gatech.edu/). You
14
- are free to use it in other platforms with adequate adjustments.
15
-
16
- # PURPOSE
17
-
18
- Simplifies submitting and tracking large BLAST jobs in cluster.
19
-
20
- # HELP
21
-
22
- 1. Files preparation:
23
-
24
- 1.1. Obtain the enveomics package in the cluster. You can use: `git clone https://github.com/lmrodriguezr/enveomics.git`
25
-
26
- 1.2. Prepare the query sequences and the database.
27
-
28
- 1.3. Copy the file `CONFIG.mock.bash` to `CONFIG.<name>.bash`, where `<name>` is a
29
- short name for your project (avoid characters other than alphanumeric).
30
-
31
- 1.4. Change the variables in `CONFIG.<name>.bash`. The **Queue and resources** and the
32
- **Pipeline** variables are very standard, and can be kept unchanged. The **Paths**
33
- variables indicate where your input files are and where the output files are to
34
- be created, so check them carefully. Finally, the **FUNCTIONS** define the core
35
- functionality of the pipeline, and should also be reviewed. By default, the
36
- Pipeline simply runs BLAST+, with default parameters and tabular output with two
37
- extra columns (qlen and slen). However, additional functionality can easily be
38
- incorporated via these functions, such as BLAST filtering, concatenation, sorting,
39
- or even execution of other programs instead of BLAST, such as BLAT, etc. Note that
40
- the output MUST be BLAST-like tabular, because this is the only format supported
41
- to check completeness and recover incomplete runs.
42
-
43
- 2. Pipeline execution:
44
-
45
- 2.1. To initialize a run, execute: `./RUNME.bash <name> run`.
46
-
47
- 2.2. To check the status of a job, execute: `./RUNME.bash <name> check`.
48
-
49
- 2.3. To pause a run, execute: `./RUNME.bash <name> pause` (see 2.1 to resume).
50
-
51
- 2.4. To check if your CONFIG defines all required parameters, execute: `./RUNME.bash <name> dry`.
52
-
53
- 2.5. To review all the e/o files in the run, execute: `./RUNME.bash <name> eo`.
54
-
55
- 3. Finalizing:
56
-
57
- 3.1. `./RUNME.bash <name> check` will inform you if a project finished. If it finished successfully,
58
- you can review your (split) results in $SCRATCH/results. If you concatenated the results in the
59
- `END` function, you should have a file with all the results in $SCRATCH/<name>.blast.
60
-
61
- 3.2. Usually, checking the e/o files at the end is a good idea (`./RUNME.bash <name> eo`). However,
62
- bear in mind that this Pipeline can overcome several errors and is robust to most failures, so
63
- don't be alarmed at the first sight of errors.
64
-
65
- # Comments
66
-
67
- * Some scripts contained in this package are actually symlinks to files in the
68
- _Scripts_ folder. Check the existance of these files when copied to
69
- the cluster.
70
-
71
- # Troubleshooting
72
-
73
- 1. Do I really have to change directory (`cd`) to the pipeline's folder everytime I want to execute
74
- something?
75
-
76
- No. Not really. For simplicity, this file tells you to execute `./RUNME.bash`. However, you don't
77
- really have to be there, you can execute it from any location. For example, if you saved enveomics in
78
- your home directory, you can just execute `~/enveomics/blast.pbs/RUNME.bash` insted from any location
79
- in the head node.
80
-
81
- 2. When I check a project, few sub-jobs are Active for much longer than the others. How do I know if those
82
- sub-jobs are really active?
83
-
84
- Lets review an example of a problematic run. When you run `./RUNME.bash <name> check`, you see the
85
- following in the "Active jobs" section:
86
- ````
87
- Idle: 155829.shared-sched.pace.gatech.edu: 02: 00: Mon Mar 17 14:10:28 EDT 2014
88
- Sub-jobs:500 Active:4 ( 0.8% ) Eligible:0 ( 0.0% ) Blocked:0 ( 0.0% ) Completed:496 ( 99.2% )
89
- Idle: 155830.shared-sched.pace.gatech.edu: 02: 00: Mon Mar 17 14:10:28 EDT 2014
90
-
91
- Running jobs: 0.
92
- Idle jobs: 2.
93
- ````
94
- That means that the job "155829.shared-sched.pace.gatech.edu" has four Active jobs, while all the others are Completed. This is
95
- a sign of something problematic. You can see the complete status of each array using
96
- `checkjob -v <JOB_NAME>`. In our example above, you would run `checkjob -v 155829`. In the output
97
- of checkjob, most jobs should report "Completed". In this example, there are four jobs that are not
98
- complete:
99
- ````
100
- ...
101
- 387 : 155829[387] : Completed
102
- 388 : 155829[388] : Running
103
- 389 : 155829[389] : Running
104
- 390 : 155829[390] : Running
105
- 391 : 155829[391] : Running
106
- 392 : 155829[392] : Completed
107
- ...
108
- ````
109
- So you can simply check these sub-jobs in more detail. For example, if I run `checkjob -v 155829[388]`,
110
- I see that the job is running in the machine `iw-k30-12.pace.gatech.edu` (Task Distribution), so I can try
111
- to login to that machine to check if the job is actually running, using `top -u $(whoami)`. However, when
112
- I run `ssh iw-k30-12`, I got a "Connection closed" error, which means that the machine hung up. At this point,
113
- you might want to try one of the following solutions:
114
-
115
- 2.1. Pause the project using `./RUNME.bash <name> pause`, wait a few minutes, and resume using
116
- `./RUNME.bash <name> run`. If you tried this a couple of times and you still have sub-jobs hanging, try:
117
-
118
- 2.2. Check if your sub-jobs finished. Sometimes sub-jobs die too soon to return a success code, but they actually
119
- finished. Just run the following command: `ls <SCRATCH>/<name>/success/02.* | wc -l`, where `<SCRATCH>` is the
120
- value you set for the `SCRATCH` variable in the CONFIG file, and `<name>` is the name of your project. If the
121
- output of that command is a number, and that number is exactly six times the number of jobs (`MAX_JOBS` in the
122
- CONFIG file, typically 500), then your step 2 actually finished. In my case, I have 500 jobs, and the output
123
- was 3000, so my job finished successfully, but the pipeline didn't notice. You can manually tell the pipeline
124
- to go on running: `touch <SCRATCH>/<name>/success/02`, and pausing/resuming the project (see 2.1 above). If
125
- the output is not the expected number (in my case, 3000, which is 6*500), DON'T RUN `touch`, just try the
126
- solution 2.1 above once again.
127
-
@@ -1,109 +0,0 @@
1
- #!/bin/bash
2
-
3
- ##################### VARIABLES
4
- # Find the directory of the pipeline
5
- if [[ "$PDIR" == "" ]] ; then PDIR=$(dirname $(readlink -f $0)); fi ;
6
- CWD=$(pwd)
7
-
8
- # Load config
9
- if [[ "$PROJ" == "" ]] ; then PROJ="$1" ; fi
10
- if [[ "$TASK" == "" ]] ; then TASK="$2" ; fi
11
- if [[ "$TASK" == "" ]] ; then TASK="check" ; fi
12
- NAMES=$(ls $PDIR/CONFIG.*.bash | sed -e 's/.*CONFIG\./ o /' | sed -e 's/\.bash//');
13
- if [[ "$PROJ" == "" ]] ; then
14
- if [[ "$HELP" == "" ]] ; then
15
- echo "
16
- Usage:
17
- $0 name task
18
-
19
- name The name of the run. CONFIG.name.bash must exist.
20
- task The action to perform. One of:
21
- o run: Executes the BLAST.
22
- o check: Indicates the progress of the task (default).
23
- o pause: Cancels running jobs (resume using run).
24
- o dry: Checks that the parameters are correct, but doesn't run.
25
- o eo: Review all eo files produced in the project.
26
-
27
- See $PDIR/README.md for more information.
28
-
29
- Available names are:
30
- $NAMES
31
- " >&2
32
- else
33
- echo "$HELP
34
- Available names are:
35
- $NAMES
36
- " >&2
37
- fi
38
- exit 1
39
- fi
40
- if [[ ! -e "$PDIR/CONFIG.$PROJ.bash" ]] ; then
41
- echo "$0: Error: Impossible to find $PDIR/CONFIG.$PROJ.bash, available names are:
42
- $NAMES" >&2
43
- exit 1
44
- fi
45
- source "$PDIR/CONFIG.$PROJ.bash" ;
46
- SCRATCH="$SCRATCH_DIR/$PROJ" ;
47
- MINVARS="PDIR=$PDIR,SCRATCH=$SCRATCH,PROJ=$PROJ" ;
48
- case $QUEUE in
49
- bioforce-6)
50
- MAX_H=120 ;;
51
- iw-shared-6)
52
- MAX_H=12 ;;
53
- micro-largedata)
54
- MAX_H=120 ;;
55
- biocluster-6 | biohimem-6 | microcluster)
56
- MAX_H=240 ;;
57
- *)
58
- echo "Unrecognized queue: $QUEUE." >&2 ;
59
- exit 1 ;;
60
- esac ;
61
-
62
- ##################### FUNCTIONS
63
- function REGISTER_JOB {
64
- local STEP=$1
65
- local SUBSTEP=$2
66
- local MESSAGE=$3
67
- local JOBID=$4
68
-
69
- if [[ "$JOBID" != "" ]] ; then
70
- MESSAGE="$MESSAGE [$JOBID]" ;
71
- echo "$STEP: $SUBSTEP: $(date)" >> "$SCRATCH/log/active/$JOBID" ;
72
- fi
73
- echo "$MESSAGE." >> "$SCRATCH/log/status/$STEP" ;
74
- }
75
-
76
- function LAUNCH_JOB {
77
- local STEP=$1
78
- local SUBSTEP=$2
79
- local MESSAGE=$3
80
- local BASHFILE=$4
81
-
82
- cd "$SCRATCH/log/eo" ;
83
- date >> "$SCRATCH/etc/trials" ;
84
- source "$BASHFILE" || exit 1 ;
85
- cd $CWD ;
86
- if [[ "$SENTINEL_JOBID" != "" ]] ; then
87
- REGISTER_JOB "$STEP" "$SUBSTEP" "Guarding job $NEW_JOBID" "$SENTINEL_JOBID" ;
88
- fi ;
89
- REGISTER_JOB "$STEP" "$SUBSTEP" "$MESSAGE" "$NEW_JOBID" ;
90
- echo $NEW_JOBID ;
91
- }
92
-
93
- function JOB_DONE {
94
- STEP=$1
95
-
96
- echo "Done." >> "$SCRATCH/log/status/$STEP" ;
97
- touch "$SCRATCH/success/$STEP" ;
98
- echo -n '# ' > "$SCRATCH/etc/trials" ;
99
- }
100
-
101
- ##################### RUN
102
- # Execute task
103
- if [[ ! -e "$PDIR/TASK.$TASK.bash" ]] ; then
104
- echo "Unrecognized task: $TASK." >&2 ;
105
- exit 1 ;
106
- else
107
- source "$PDIR/TASK.$TASK.bash"
108
- fi
109
-
@@ -1,128 +0,0 @@
1
- #!/bin/bash
2
-
3
- ##################### RUN
4
- # Check if it was sourced from RUNME.bash
5
- if [[ "$PDIR" == "" ]] ; then
6
- echo "$0: Error: This file is not stand-alone." >&2
7
- echo " Execute RUNME.bash as described in the README.txt file" >&2
8
- exit 1
9
- fi
10
-
11
- # Check if the project exists
12
- if [[ ! -d "$SCRATCH" ]] ; then
13
- echo "The project $PROJ doesn't exist at $SCRATCH_DIR." >&2
14
- echo " Execute '$PDIR/RUNME.bash $PROJ run' first." >&2
15
- exit 1
16
- fi
17
-
18
- # Get log:
19
- echo "==[ Running tasks ]=="
20
- for i in $(ls $SCRATCH/log/status/* 2>/dev/null) ; do
21
- echo " $(basename $i): $(tail -n 1 $i)"
22
- done
23
- echo ""
24
-
25
- # Get active jobs:
26
- echo "==[ Active jobs ]=="
27
- job_r=0
28
- job_i=0
29
- job_c=0
30
- for i in $(ls $SCRATCH/log/active/* 2>/dev/null) ; do
31
- jid=$(basename $i)
32
- stat=$(qstat -f1 $jid 2>&1)
33
- state=$(echo "$stat" | grep '^ *job_state = ' | sed -e 's/.*job_state = //')
34
- case $state in
35
- C)
36
- code=$(echo "$stat" | grep '^ *exit_status = ' | sed -e 's/.*exit_status = //')
37
- if [[ "$code" == "0" ]] ; then
38
- mv "$i" "$SCRATCH/log/done/"
39
- let job_c=$job_c+1
40
- else
41
- echo "Warning: Job $jid ($(cat $i|tr -d '\n')) failed with code $code." >&2
42
- echo " see errors at: $(echo "$stat" | grep '^ *Error_Path = ' | sed -e 's/.*Error_Path = //')"
43
- mv "$i" "$SCRATCH/log/failed/"
44
- fi ;;
45
- R)
46
- echo " Running: $jid: $(cat "$i")"
47
- let job_r=$job_r+1 ;;
48
- [HQW])
49
- echo " Idle: $jid: $(cat "$i")"
50
- let job_i=$job_i+1 ;;
51
- E)
52
- echo " Canceling: $jid: $(cat "$i")" ;;
53
- *)
54
- tmp_err=$(echo "$stat" | grep ERROR)
55
- if [[ "$tmp_err" == "" ]] ; then
56
- echo "Warning: Unrecognized state: $jid: $state." >&2
57
- echo " Please report this problem." >&2
58
- else
59
- echo " Error: $jid: $tmp_err"
60
- fi ;;
61
- esac
62
- #subjobs=$(echo "$stat" | grep 'Sub-jobs:' | sed -e 's/.*: *//')
63
- #if [[ "$subjobs" -gt 0 ]] ; then
64
- # echo "$stat" | grep '^ *\(Sub-jobs\|Active\|Eligible\|Blocked\|Completed\):' | sed -e 's/^ *//' | sed -e 's/ *//' | tr '\n' ' ' | sed -e 's/^/ /'
65
- # echo
66
- #fi
67
- done
68
- if [[ $job_c -gt 0 ]] ; then
69
- echo ""
70
- echo " Completed since last check: $job_c."
71
- fi
72
- if [[ $job_r -gt 0 || $job_i -gt 0 ]] ; then
73
- echo ""
74
- echo " Running jobs: $job_r."
75
- echo " Idle jobs: $job_i."
76
- fi
77
- echo ""
78
-
79
- # Auto-trials
80
- echo "==[ Auto-trials ]=="
81
- if [[ -e "$SCRATCH/etc/trials" ]] ; then
82
- trials=$(cat "$SCRATCH/etc/trials" | wc -l | sed -e 's/ //g')
83
- if [[ $trials -gt 1 ]] ; then
84
- echo " $trials trials attempted:"
85
- else
86
- echo " No recent failures in the current step, job launched:"
87
- fi
88
- cat "$SCRATCH/etc/trials" | sed -e 's/^/ o /' | sed -e 's/# $/No active trials\n/g'
89
- fi
90
- echo ""
91
-
92
- # Step-specific checks:
93
- echo "==[ Step summary ]=="
94
- todo=1
95
- if [[ -e "$SCRATCH/success/00" ]] ; then
96
- echo " Successful project initialization."
97
- if [[ -e "$SCRATCH/success/01" ]] ; then
98
- echo " Successful input preparation."
99
- if [[ -e "$SCRATCH/success/02" ]] ; then
100
- echo " Successful BLAST execution."
101
- if [[ -e "$SCRATCH/success/02" ]] ; then
102
- echo " Successful concatenation."
103
- echo " Project finished successfully!"
104
- todo=0
105
- else
106
- echo " Concatenating results."
107
- fi
108
- else
109
- echo " Running BLAST."
110
- fi
111
- else
112
- echo " Preparing input."
113
- fi
114
- else
115
- echo " Initializing project."
116
- fi
117
-
118
- if [[ "$todo" -eq 1 && $job_r -eq 0 && $job_i -eq 0 ]] ; then
119
- echo " Job currently paused. To resume, execute:"
120
- echo " $PDIR/RUNME.bash $PROJ run"
121
- fi
122
- echo
123
-
124
- # Entire log
125
- echo "==[ Complete log ]=="
126
- for i in $(ls $SCRATCH/log/status/* 2>/dev/null) ; do
127
- cat "$i" | sed -e "s/^/ $(basename $i): /"
128
- done
@@ -1,16 +0,0 @@
1
-
2
- [[ "$QUEUE" == "" ]] && echo "Undefined QUEUE" >&2 && exit 1;
3
- [[ "$MAX_JOBS" == "" ]] && echo "Undefined MAX_JOBS" >&2 && exit 1;
4
- [[ "$PPN" == "" ]] && echo "Undefined PPN" >&2 && exit 1;
5
- [[ "$RAM" == "" ]] && echo "Undefined RAM" >&2 && exit 1;
6
- [[ "$SCRATCH_DIR" == "" ]] && echo "Undefined SCRATCH_DIR" >&2 && exit 1;
7
- [[ "$INPUT" == "" ]] && echo "Undefined INPUT" >&2 && exit 1;
8
- [[ "$DB" == "" ]] && echo "Undefined DB" >&2 && exit 1;
9
- [[ "$PROGRAM" == "" ]] && echo "Undefined PROGRAM" >&2 && exit 1;
10
- [[ "$MAX_TRIALS" == "" ]] && echo "Undefined MAX_TRIALS" >&2 && exit 1;
11
- [[ "$(type -t BEGIN)" == "function" ]] || ( echo "Undefined function BEGIN" && exit 1 ) ;
12
- [[ "$(type -t BEFORE_BLAST)" == "function" ]] || ( echo "Undefined function BEFORE_BLAST" && exit 1 ) ;
13
- [[ "$(type -t RUN_BLAST)" == "function" ]] || ( echo "Undefined function RUN_BLAST" && exit 1 ) ;
14
- [[ "$(type -t AFTER_BLAST)" == "function" ]] || ( echo "Undefined function AFTER_BLAST" && exit 1 ) ;
15
- [[ "$(type -t END)" == "function" ]] || ( echo "Undefined function END" && exit 1 ) ;
16
-
@@ -1,22 +0,0 @@
1
- #!/bin/bash
2
-
3
- ##################### RUN
4
- # Check if it was sourced from RUNME.bash
5
- if [[ "$PDIR" == "" ]] ; then
6
- echo "$0: Error: This file is not stand-alone." >&2
7
- echo " Execute RUNME.bash as described in the README.txt file" >&2 ;
8
- exit 1 ;
9
- fi ;
10
-
11
- # Check if the project exists
12
- if [[ ! -d "$SCRATCH" ]] ; then
13
- echo "The project $PROJ doesn't exist at $SCRATCH_DIR." >&2 ;
14
- echo " Execute '$PDIR/RUNME.bash $PROJ run' first." >&2 ;
15
- exit 1 ;
16
- fi ;
17
-
18
- # Review errors
19
- (echo -e "==[ Last 10 lines of all e files ]==\nPress q to exit\n" ; tail -n 10 $SCRATCH/log/eo/*.e* ) | less
20
- # Review output
21
- (echo -e "==[ Last 100 lines of all o files ]==\nPress q to exit\n" ; tail -n 100 $SCRATCH/log/eo/*.o* ) | less
22
-
@@ -1,26 +0,0 @@
1
- #!/bin/bash
2
-
3
- ##################### RUN
4
- # Check if it was sourced from RUNME.bash
5
- if [[ "$PDIR" == "" ]] ; then
6
- echo "$0: Error: This file is not stand-alone." >&2
7
- echo " Execute RUNME.bash as described in the README.txt file" >&2 ;
8
- exit 1 ;
9
- fi ;
10
-
11
- # Get active jobs:
12
- echo "======[ check ]======"
13
- job_r=0;
14
- job_i=0;
15
- job_c=0;
16
-
17
- echo "======[ pause ]======"
18
- for i in $(ls $SCRATCH/log/active/* 2>/dev/null) ; do
19
- echo " Pausing $jid." ;
20
- jid=$(basename $i) ;
21
- qdel $jid ;
22
- done ;
23
-
24
- # Restart auto-trials
25
- echo -n > "$SCRATCH/etc/trials" ;
26
-
@@ -1,89 +0,0 @@
1
- #!/bin/bash
2
-
3
- ##################### RUN
4
- # Check if it was sourced from RUNME.bash
5
- if [[ "$PDIR" == "" ]] ; then
6
- echo "$0: Error: This file is not stand-alone. Execute RUNME.bash as described in the README.txt file" >&2
7
- exit 1
8
- fi
9
-
10
- # Check if too many auto-trials were attempted
11
- trials=0 ;
12
- if [[ -e "$SCRATCH/etc/trials" ]] ; then
13
- trials=$(cat "$SCRATCH/etc/trials" | wc -l | sed -e 's/ //g');
14
- if [[ $trials -ge $MAX_TRIALS ]] ; then
15
- echo "The maximum number of trials was already attempted, halting." >&2 ;
16
- exit 1 ;
17
- fi ;
18
- fi ;
19
-
20
- # Create the scratch directory
21
- if [[ ! -d "$SCRATCH" ]] ; then mkdir -p "$SCRATCH" || exit 1 ; fi;
22
-
23
- if [[ ! -e "$SCRATCH/success/00" ]] ; then
24
- # 00. Initialize the project
25
- echo "00. Initializing project." >&2 ;
26
- mkdir -p "$SCRATCH/tmp" "$SCRATCH/etc" "$SCRATCH/results" "$SCRATCH/success" || exit 1 ;
27
- mkdir -p "$SCRATCH/log/active" "$SCRATCH/log/done" "$SCRATCH/log/failed" || exit 1 ;
28
- mkdir -p "$SCRATCH/log/status" "$SCRATCH/log/eo" || exit 1 ;
29
- echo "Preparing structure." >> "$SCRATCH/log/status/00" ;
30
- # Build 01.bash
31
- echo "NEW_JOBID=\$(qsub -q '$QUEUE' -l 'walltime=$MAX_H:00:00,mem=$RAM' -v '$MINVARS' -N '$PROJ-01' \\
32
- '$PDIR/01.pbs.bash'|tr -d '\\n')" \
33
- > "$SCRATCH/etc/01.bash" || exit 1 ;
34
- echo "SENTINEL_JOBID=\$(qsub -q '$QUEUE' -l 'walltime=2:00:00' -W \"depend=afterany:\$NEW_JOBID\" \\
35
- -v \"$MINVARS,STEP=01,AFTERJOB=\$NEW_JOBID\" -N '$PROJ-01-sentinel' '$PDIR/sentinel.pbs.bash'|tr -d '\\n')" \
36
- >> "$SCRATCH/etc/01.bash" || exit 1 ;
37
- # Build 02.bash
38
- echo "NEW_JOBID=\$(qsub -q '$QUEUE' -l 'walltime=$MAX_H:00:00,mem=$RAM,nodes=1:ppn=$PPN' \\
39
- -v '$MINVARS' -N '$PROJ-02' -t '1-$MAX_JOBS' '$PDIR/02.pbs.bash'|tr -d '\\n')" \
40
- > "$SCRATCH/etc/02.bash" \
41
- || exit 1 ;
42
- echo "SENTINEL_JOBID=\$(qsub -q '$QUEUE' -l 'walltime=2:00:00' -W \"depend=afteranyarray:\$NEW_JOBID\" \\
43
- -v \"$MINVARS,STEP=02,AFTERJOB=\$NEW_JOBID\" -N '$PROJ-02-sentinel' '$PDIR/sentinel.pbs.bash'|tr -d '\\n')" \
44
- >> "$SCRATCH/etc/02.bash" \
45
- || exit 1 ;
46
- # Build 03.bash
47
- echo "NEW_JOBID=\$(qsub -q '$QUEUE' -l 'walltime=$MAX_H:00:00,mem=$RAM' -v '$MINVARS' -N '$PROJ-03' \\
48
- '$PDIR/03.pbs.bash'|tr -d '\\n')" \
49
- > "$SCRATCH/etc/03.bash" || exit 1 ;
50
- echo "SENTINEL_JOBID=\$(qsub -q '$QUEUE' -l 'walltime=2:00:00' -W \"depend=afterany:\$NEW_JOBID\" \\
51
- -v \"$MINVARS,STEP=03,AFTERJOB=\$NEW_JOBID\" -N '$PROJ-03-sentinel' '$PDIR/sentinel.pbs.bash'|tr -d '\\n')" \
52
- >> "$SCRATCH/etc/03.bash" || exit 1 ;
53
-
54
- JOB_DONE "00" ;
55
- fi ;
56
-
57
- if [[ ! -e "$SCRATCH/success/01" ]] ; then
58
- # 01. Preparing input
59
- echo "01. Preparing input." >&2 ;
60
- JOB01=$(LAUNCH_JOB "01" "00" "Preparing input files" "$SCRATCH/etc/01.bash") ;
61
- echo " New job: $JOB01." >&2 ;
62
- else
63
- if [[ ! -e "$SCRATCH/success/02" ]] ; then
64
- # 02. Launching BLAST
65
- echo "02. Launching BLAST." >&2 ;
66
- JOB02=$(LAUNCH_JOB "02" "00" "Running BLAST" "$SCRATCH/etc/02.bash") ;
67
- echo " New job: $JOB02." >&2 ;
68
- # Clean on resubmission
69
- cleaned=0
70
- echo -n " Cleaning completed sub-jobs on $JOB02: " >&2 ;
71
- for jobi in $(seq 1 $MAX_JOBS) ; do
72
- if [[ -e "$SCRATCH/success/02.$jobi" ]] ; then
73
- qdel "$JOB02""[$jobi]" &> /dev/null ;
74
- let cleaned=$cleaned+1 ;
75
- fi ;
76
- done ;
77
- echo "$cleaned sub-jobs completed." >&2 ;
78
- else
79
- if [[ ! -e "$SCRATCH/success/03" ]] ; then
80
- # 03. Finalize
81
- echo "03. Finalizing." >&2 ;
82
- JOB03=$(LAUNCH_JOB "03" "00" "Concatenating results" "$SCRATCH/etc/03.bash") ;
83
- echo " New job: $JOB03." >&2 ;
84
- else
85
- echo "Project complete, nothing to run." ;
86
- fi ;
87
- fi ;
88
- fi ;
89
-
@@ -1,29 +0,0 @@
1
- # blast.pbs pipeline
2
- # Sentinel script
3
-
4
- echo "Sentinel script after $AFTERJOB" ;
5
-
6
- # Step-specific checks
7
- if [[ "$STEP" == "02" ]] ; then
8
- # Read configuration
9
- cd $SCRATCH ;
10
- TASK="dry" ;
11
- source "$PDIR/RUNME.bash" ;
12
-
13
- # Check tasks
14
- INCOMPLETE=0;
15
- for i in $(seq 1 $MAX_JOBS) ; do
16
- if [[ ! -e "$SCRATCH/success/02.$i" ]] ; then
17
- let INCOMPLETE=$INCOMPLETE+1 ;
18
- fi ;
19
- done
20
- if [[ $INCOMPLETE -eq 0 ]] ; then
21
- JOB_DONE "02" ;
22
- else
23
- echo "$INCOMPLETE incomplete jobs, re-launching step 02." ;
24
- fi ;
25
- fi
26
-
27
- # Continue the workflow
28
- "$PDIR/RUNME.bash" "$PROJ" run || exit 1 ;
29
-
@@ -1,49 +0,0 @@
1
- @author: Luis Miguel Rodriguez-R <lmrodriguezr at gmail dot com>
2
-
3
- @update: Feb-26-2015
4
-
5
- @license: artistic 2.0
6
-
7
- @status: auto
8
-
9
- @pbs: yes
10
-
11
- # IMPORTANT
12
-
13
- This pipeline was developed for the [PACE cluster](http://pace.gatech.edu/). You
14
- are free to use it in other platforms with adequate adjustments.
15
-
16
- # PURPOSE
17
-
18
- Performs assembly using IDBA-UD, designed for Single-Cell Genomics and Metagenomics.
19
-
20
- # HELP
21
-
22
- 1. Files preparation:
23
-
24
- 1.1. Obtain the enveomics package in the cluster. You can use:
25
- `git clone https://github.com/lmrodriguezr/enveomics.git`
26
-
27
- 1.2. Prepare the trimmed reads (e.g., use trim.bs) in interposed FastA format. Files
28
- must be raw, not zipped or packaged. Filenames must conform the format:
29
- <name>.CoupledReads.fa, where <name> is the name of the sample. Locate all the
30
- files within a folder named 04.trimmed_fasta, within your project folder. If you
31
- used trim.pbs, no further action is necessary.
32
-
33
- 2. Pipeline execution:
34
-
35
- 2.1. Simply execute `./RUNME.bash <dir> <data_type>`, where `<dir>` is the folder containing
36
- the 04.trimmed_fasta folder, and `<data_type>` is a supported type of data (see help
37
- message running `./RUNME.bash` without arguments).
38
-
39
- 3. What to expect:
40
-
41
- By the end of the run, you should find the folder *05.assembly*, including the following
42
- files for each dataset:
43
-
44
- 3.1. `<dataset>`: The IDBA output folder.
45
-
46
- 3.2. `<dataset>.AllContigs.fna`: All contigs longer than 200bp in FastA format.
47
-
48
- 3.2. `<dataset>.LargeContigs.fna`: Contigs longer than 500bp in FastA format.
49
-