miga-base 1.2.14.2 → 1.2.15.1
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- checksums.yaml +4 -4
- data/lib/miga/cli/action/doctor/base.rb +16 -40
- data/lib/miga/cli/action/doctor/databases.rb +39 -0
- data/lib/miga/cli/action/doctor/distances.rb +144 -0
- data/lib/miga/cli/action/doctor/operations.rb +159 -0
- data/lib/miga/cli/action/doctor.rb +7 -287
- data/lib/miga/cli/action/download/base.rb +64 -2
- data/lib/miga/cli/action/gtdb_get.rb +2 -31
- data/lib/miga/cli/action/ncbi_get.rb +6 -31
- data/lib/miga/cli/opt_helper.rb +1 -1
- data/lib/miga/common/errors.rb +10 -0
- data/lib/miga/dataset/base.rb +34 -5
- data/lib/miga/dataset/result/add.rb +286 -0
- data/lib/miga/dataset/result/ignore.rb +93 -0
- data/lib/miga/dataset/result.rb +31 -342
- data/lib/miga/remote_dataset/download.rb +6 -0
- data/lib/miga/version.rb +2 -2
- data/test/remote_dataset_test.rb +6 -0
- metadata +7 -2
data/lib/miga/dataset/result.rb
CHANGED
@@ -1,3 +1,5 @@
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1
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+
# frozen_string_literal: true
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2
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+
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1
3
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require 'miga/result'
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2
4
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require 'miga/dataset/base'
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3
5
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require 'miga/common/with_result'
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@@ -13,56 +15,17 @@ module MiGA::Dataset::Result
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13
15
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include MiGA::Dataset::Base
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14
16
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include MiGA::Common::WithResult
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15
17
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18
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+
require 'miga/dataset/result/ignore'
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19
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+
require 'miga/dataset/result/add'
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20
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+
include MiGA::Dataset::Result::Ignore
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+
include MiGA::Dataset::Result::Add
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22
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+
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16
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##
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# Return the basename for results
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def result_base
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name
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27
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end
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28
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22
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-
##
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23
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-
# Should I ignore +task+ for this dataset?
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24
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-
def ignore_task?(task)
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25
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why_ignore(task) != :execute
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26
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-
end
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-
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28
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##
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29
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# Return a code explaining why a task is ignored.
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30
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# The values are symbols:
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31
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-
# - empty: the dataset has no data
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32
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-
# - inactive: the dataset is inactive
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33
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-
# - upstream: the task is upstream from dataset's input
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34
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-
# - force: forced to ignore by metadata
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35
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-
# - project: incompatible project
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36
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-
# - noref: incompatible dataset, only for reference
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37
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-
# - multi: incompatible dataset, only for multi
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38
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-
# - nonmulti: incompatible dataset, only for nonmulti
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39
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-
# - complete: the task is already complete
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40
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-
# - execute: do not ignore, execute the task
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41
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-
def why_ignore(task)
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42
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-
if !get_result(task).nil?
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-
:complete
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44
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-
elsif !active?
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45
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-
:inactive
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46
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-
elsif first_preprocessing.nil?
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47
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-
:empty
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48
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-
elsif @@PREPROCESSING_TASKS.index(task) <
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49
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-
@@PREPROCESSING_TASKS.index(first_preprocessing)
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50
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-
:upstream
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51
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-
elsif !metadata["run_#{task}"].nil?
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52
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metadata["run_#{task}"] ? :execute : :force
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53
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-
elsif task == :taxonomy && project.option(:ref_project).nil?
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54
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-
:project
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55
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-
elsif @@_EXCLUDE_NOREF_TASKS_H[task] && !ref?
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56
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-
:noref
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57
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-
elsif @@_ONLY_MULTI_TASKS_H[task] && !multi?
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58
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-
:multi
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59
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-
elsif @@_ONLY_NONMULTI_TASKS_H[task] && !nonmulti?
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60
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-
:nonmulti
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-
else
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-
:execute
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63
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-
end
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-
end
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-
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29
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##
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# Returns the key symbol of the first registered result (sorted by the
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68
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# execution order). This typically corresponds to the result used as the
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@@ -95,18 +58,18 @@ module MiGA::Dataset::Result
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# - 2 for a queued result (a task yet to be executed).
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59
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# It passes +save+ to #add_result
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60
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def profile_advance(save = false)
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98
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-
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99
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-
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61
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+
# Determine the start point
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+
first_task = first_preprocessing(save) or
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63
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+
return Array.new(self.class.PREPROCESSING_TASKS.size, 0)
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100
64
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101
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-
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65
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+
# Traverse all tasks and determine the corresponding state
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66
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state = 0
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67
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next_task = next_preprocessing(save)
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104
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-
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68
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+
self.class.PREPROCESSING_TASKS.map do |task|
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69
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state = 1 if first_task == task
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70
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state = 2 if !next_task.nil? && next_task == task
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-
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+
state
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72
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end
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-
adv
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73
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end
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74
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75
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##
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@@ -125,9 +88,9 @@ module MiGA::Dataset::Result
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125
88
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def result_status(task)
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126
89
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reason = why_ignore(task)
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90
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case reason
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128
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-
when :upstream
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129
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-
when :execute
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130
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-
when :complete
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91
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+
when :upstream then :-
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92
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when :execute then :pending
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93
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when :complete then :complete
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94
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else; :"ignore_#{reason}"
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95
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end
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96
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end
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@@ -136,306 +99,32 @@ module MiGA::Dataset::Result
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99
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# Clean-up all the stored distances, removing values for datasets no longer in
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137
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# the project as reference datasets.
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101
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def cleanup_distances!
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139
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-
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140
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-
return if r.nil?
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102
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+
return if get_result(:distances).nil?
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141
103
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|
142
104
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require 'miga/sqlite'
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143
105
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ref = project.datasets.select(&:ref?).select(&:active?).map(&:name)
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144
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%i[
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145
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-
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146
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next if db.nil? || !File.size?(db)
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147
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-
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148
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sqlite_db = MiGA::SQLite.new(db)
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149
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table = db_type[-6..-4]
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150
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val = sqlite_db.run("select seq2 from #{table}")
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151
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next if val.empty?
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152
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-
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153
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(val.map(&:first) - ref).each do |extra|
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154
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sqlite_db.run("delete from #{table} where seq2=?", extra)
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155
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-
end
|
106
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+
%i[haai aai ani].each do |metric|
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107
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+
cleanup_distances_by_metric!(ref, metric)
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156
108
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end
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157
109
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end
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private
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112
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113
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##
|
162
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-
#
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-
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164
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-
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165
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-
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166
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-
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167
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-
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168
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if result_files_exist?(base, '.2.fastq')
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169
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{ pair1: '.1.fastq', pair2: '.2.fastq' }
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170
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-
else
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171
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{ single: '.1.fastq' }
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172
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-
end
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)
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174
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-
end
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175
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-
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176
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##
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177
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-
# Add result type +:trimmed_reads+ at +base+ (no +_opts+ supported)
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-
def add_result_trimmed_reads(base, _opts)
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179
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return nil unless result_files_exist?(base, '.1.clipped.fastq')
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180
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-
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181
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add_files_to_ds_result(
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182
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MiGA::Result.new("#{base}.json"), name,
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183
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if result_files_exist?(base, '.2.clipped.fastq')
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184
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{ pair1: '.1.clipped.fastq', pair2: '.2.clipped.fastq' }
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185
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-
else
|
186
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{ single: '.1.clipped.fastq' }
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187
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-
end
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188
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).tap do |r|
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189
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# Legacy files
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190
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r.add_file(:trimming_sumary, "#{name}.1.fastq.trimmed.summary.txt")
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191
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-
r.add_file(:single, "#{name}.1.clipped.single.fastq")
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192
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-
end
|
193
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-
end
|
194
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-
|
195
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-
##
|
196
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-
# Add result type +:read_quality+ at +base+ (no +_opts+ supported)
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197
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-
def add_result_read_quality(base, _opts)
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198
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-
return nil unless
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199
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-
result_files_exist?(base, %w[.post.1.html]) ||
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200
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-
result_files_exist?(base, %w[.solexaqa .fastqc])
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201
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-
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202
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add_files_to_ds_result(
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203
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MiGA::Result.new("#{base}.json"), name,
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pre_qc_1: '.pre.1.html', pre_qc_2: '.pre.2.html',
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post_qc_1: '.post.1.html', post_qc_2: '.post.2.html',
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adapter_detection: '.adapters.txt',
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# Legacy files
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solexaqa: '.solexaqa', fastqc: '.fastqc'
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209
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)
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210
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end
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211
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-
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212
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##
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# Add result type +:trimmed_fasta+ at +base+ (no +_opts+ supported)
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214
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-
def add_result_trimmed_fasta(base, _opts)
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215
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-
return nil unless
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216
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-
result_files_exist?(base, '.CoupledReads.fa') ||
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217
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-
result_files_exist?(base, '.SingleReads.fa') ||
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218
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-
result_files_exist?(base, %w[.1.fasta .2.fasta])
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219
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-
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220
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add_files_to_ds_result(
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221
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MiGA::Result.new("#{base}.json"), name,
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coupled: '.CoupledReads.fa',
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single: '.SingleReads.fa',
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pair1: '.1.fasta',
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225
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pair2: '.2.fasta'
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)
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end
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-
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##
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# Add result type +:assembly+ at +base+. Hash +opts+ supports
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# +is_clean: Boolean+.
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232
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-
def add_result_assembly(base, opts)
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233
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return nil unless result_files_exist?(base, '.LargeContigs.fna')
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234
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-
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235
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r = add_files_to_ds_result(
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236
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MiGA::Result.new("#{base}.json"), name,
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largecontigs: '.LargeContigs.fna',
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allcontigs: '.AllContigs.fna',
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assembly_data: ''
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)
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241
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opts[:is_clean] ||= false
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242
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r.clean! if opts[:is_clean]
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243
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unless r.clean?
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244
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MiGA::MiGA.clean_fasta_file(r.file_path(:largecontigs))
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r.clean!
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end
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r
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end
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-
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250
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##
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251
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# Add result type +:cds+ at +base+. Hash +opts+ supports +is_clean: Boolean+
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252
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-
def add_result_cds(base, opts)
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253
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-
return nil unless result_files_exist?(base, %w[.faa])
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254
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-
|
255
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r = add_files_to_ds_result(
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256
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MiGA::Result.new("#{base}.json"), name,
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257
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proteins: '.faa',
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258
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genes: '.fna',
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259
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gff2: '.gff2',
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260
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gff3: '.gff3',
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261
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tab: '.tab'
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262
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-
)
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263
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opts[:is_clean] ||= false
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264
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r.clean! if opts[:is_clean]
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265
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-
unless r.clean?
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266
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MiGA::MiGA.clean_fasta_file(r.file_path(:proteins))
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267
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MiGA::MiGA.clean_fasta_file(r.file_path(:genes)) if r.file_path(:genes)
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268
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r.clean!
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269
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-
end
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270
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r
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271
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-
end
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272
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-
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273
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-
##
|
274
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-
# Add result type +:essential_genes+ at +base+ (no +_opts+ supported).
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275
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-
def add_result_essential_genes(base, _opts)
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276
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-
return nil unless result_files_exist?(base, %w[.ess.faa .ess .ess/log])
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114
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+
# Cleanup the tables of a specific +metric+ (symbol) removing all values
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115
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+
# against dataset names not in +ref+ (Array of string)
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+
def cleanup_distances_by_metric!(ref, metric)
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+
db_type = :"#{metric}_db"
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db = get_result(:distances).file_path(db_type)
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+
return if db.nil? || !File.size?(db)
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120
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278
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-
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279
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-
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280
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-
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281
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-
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282
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report: '.ess/log',
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283
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alignments: '.ess/proteins.aln',
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284
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fastaai_index: '.faix.db.gz',
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285
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fastaai_index_2: '.faix'
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286
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)
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287
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-
end
|
288
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-
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289
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-
##
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290
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-
# Add result type +:ssu+ at +base+. Hash +opts+ supports +is_clean: Boolean+
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291
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-
def add_result_ssu(base, opts)
|
292
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-
return MiGA::Result.new("#{base}.json") if result(:assembly).nil?
|
293
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-
return nil unless result_files_exist?(base, '.ssu.fa')
|
294
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-
|
295
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-
r = add_files_to_ds_result(
|
296
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MiGA::Result.new("#{base}.json"), name,
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297
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-
longest_ssu_gene: '.ssu.fa',
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298
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-
ssu_gff: '.ssu.gff', # DEPRECATED
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299
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gff: '.gff',
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300
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all_ssu_genes: '.ssu.all.fa',
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301
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classification: '.rdp.tsv',
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302
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trna_list: '.trna.txt'
|
303
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-
)
|
304
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opts[:is_clean] ||= false
|
305
|
-
r.clean! if opts[:is_clean]
|
306
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-
unless r.clean?
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307
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-
MiGA::MiGA.clean_fasta_file(r.file_path(:longest_ssu_gene))
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308
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-
r.clean!
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309
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-
end
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310
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-
r
|
311
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-
end
|
312
|
-
|
313
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-
##
|
314
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-
# Add result type +:mytaxa+ at +base+ (no +_opts+ supported)
|
315
|
-
def add_result_mytaxa(base, _opts)
|
316
|
-
if multi?
|
317
|
-
return nil unless
|
318
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-
result_files_exist?(base, '.mytaxa') ||
|
319
|
-
result_files_exist?(base, '.nomytaxa.txt')
|
320
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-
|
321
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-
add_files_to_ds_result(
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322
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MiGA::Result.new("#{base}.json"), name,
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323
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mytaxa: '.mytaxa',
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324
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blast: '.blast',
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325
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mytaxain: '.mytaxain',
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326
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-
nomytaxa: '.nomytaxa.txt',
|
327
|
-
species: '.mytaxa.Species.txt',
|
328
|
-
genus: '.mytaxa.Genus.txt',
|
329
|
-
phylum: '.mytaxa.Phylum.txt',
|
330
|
-
innominate: '.mytaxa.innominate',
|
331
|
-
kronain: '.mytaxa.krona',
|
332
|
-
krona: '.html'
|
333
|
-
)
|
334
|
-
else
|
335
|
-
MiGA::Result.new("#{base}.json")
|
336
|
-
end
|
337
|
-
end
|
338
|
-
|
339
|
-
##
|
340
|
-
# Add result type +:mytaxa_scan+ at +base+ (no +_opts+ supported)
|
341
|
-
def add_result_mytaxa_scan(base, _opts)
|
342
|
-
if nonmulti?
|
343
|
-
return nil unless
|
344
|
-
result_files_exist?(base, %w[.pdf .mytaxa]) ||
|
345
|
-
result_files_exist?(base, '.nomytaxa.txt')
|
346
|
-
|
347
|
-
add_files_to_ds_result(
|
348
|
-
MiGA::Result.new("#{base}.json"), name,
|
349
|
-
nomytaxa: '.nomytaxa.txt',
|
350
|
-
mytaxa: '.mytaxa',
|
351
|
-
report: '.pdf',
|
352
|
-
regions_archive: '.reg.tar',
|
353
|
-
# Intermediate / Deprecated:
|
354
|
-
blast: '.blast',
|
355
|
-
mytaxain: '.mytaxain',
|
356
|
-
wintax: '.wintax',
|
357
|
-
gene_ids: '.wintax.genes',
|
358
|
-
region_ids: '.wintax.regions',
|
359
|
-
regions: '.reg'
|
360
|
-
)
|
361
|
-
else
|
362
|
-
MiGA::Result.new("#{base}.json")
|
363
|
-
end
|
364
|
-
end
|
365
|
-
|
366
|
-
##
|
367
|
-
# Add result type +:distances+ at +base+ (no +_opts+ supported)
|
368
|
-
def add_result_distances(base, _opts)
|
369
|
-
if nonmulti?
|
370
|
-
if ref?
|
371
|
-
add_result_distances_ref(base)
|
372
|
-
else
|
373
|
-
add_result_distances_nonref(base)
|
374
|
-
end
|
375
|
-
else
|
376
|
-
add_result_distances_multi(base)
|
377
|
-
end
|
378
|
-
end
|
379
|
-
|
380
|
-
##
|
381
|
-
# Add result type +:taxonomy+ at +base+ (no +_opts+ supported)
|
382
|
-
def add_result_taxonomy(base, _opts)
|
383
|
-
add_result_distances_nonref(base)
|
384
|
-
end
|
385
|
-
|
386
|
-
##
|
387
|
-
# Add result type +:stats+ at +base+ (no +_opts+ supported)
|
388
|
-
def add_result_stats(base, _opts)
|
389
|
-
MiGA::Result.new("#{base}.json")
|
390
|
-
end
|
391
|
-
|
392
|
-
##
|
393
|
-
# Add result type +:distances+ for _multi_ datasets at +base+
|
394
|
-
def add_result_distances_multi(base)
|
395
|
-
MiGA::Result.new("#{base}.json")
|
396
|
-
end
|
121
|
+
sqlite_db = MiGA::SQLite.new(db)
|
122
|
+
table = db_type[-6..-4]
|
123
|
+
val = sqlite_db.run("select seq2 from #{table}")
|
124
|
+
return if val.empty?
|
397
125
|
|
398
|
-
|
399
|
-
|
400
|
-
def add_result_distances_ref(base)
|
401
|
-
pref = File.dirname(base)
|
402
|
-
return nil unless File.exist?("#{pref}/01.haai/#{name}.db")
|
403
|
-
|
404
|
-
MiGA::Result.new("#{base}.json").tap do |r|
|
405
|
-
r.add_files(
|
406
|
-
haai_db: "01.haai/#{name}.db",
|
407
|
-
aai_db: "02.aai/#{name}.db",
|
408
|
-
ani_db: "03.ani/#{name}.db"
|
409
|
-
)
|
126
|
+
(val.map(&:first) - ref).each do |extra|
|
127
|
+
sqlite_db.run("delete from #{table} where seq2=?", extra)
|
410
128
|
end
|
411
129
|
end
|
412
|
-
|
413
|
-
##
|
414
|
-
# Add result type +:distances+ for _nonmulti_ query datasets at +base+
|
415
|
-
def add_result_distances_nonref(base)
|
416
|
-
return nil unless
|
417
|
-
result_files_exist?(base, %w[.aai-medoids.tsv .aai.db]) ||
|
418
|
-
result_files_exist?(base, %w[.ani-medoids.tsv .ani.db])
|
419
|
-
|
420
|
-
add_files_to_ds_result(
|
421
|
-
MiGA::Result.new("#{base}.json"), name,
|
422
|
-
aai_medoids: '.aai-medoids.tsv',
|
423
|
-
haai_db: '.haai.db',
|
424
|
-
aai_db: '.aai.db',
|
425
|
-
ani_medoids: '.ani-medoids.tsv',
|
426
|
-
ani_db: '.ani.db',
|
427
|
-
ref_tree: '.nwk',
|
428
|
-
ref_tree_pdf: '.nwk.pdf',
|
429
|
-
intax_test: '.intax.txt'
|
430
|
-
)
|
431
|
-
end
|
432
|
-
|
433
|
-
##
|
434
|
-
# Add files in +rel_files+ Hash to the result +r+ with dataset name +name+
|
435
|
-
def add_files_to_ds_result(r, name, rel_files)
|
436
|
-
files = {}
|
437
|
-
rel_files.each { |k, v| files[k] = name + v }
|
438
|
-
r.add_files(files)
|
439
|
-
r
|
440
|
-
end
|
441
130
|
end
|
@@ -43,6 +43,12 @@ class MiGA::RemoteDataset
|
|
43
43
|
# +format+: String, passed to download
|
44
44
|
def ncbi_asm_rest(opts)
|
45
45
|
url_dir = opts[:obj].ncbi_asm_json_doc['ftppath_genbank']
|
46
|
+
if url_dir.nil? || url_dir.empty?
|
47
|
+
raise MiGA::RemoteDataMissingError.new(
|
48
|
+
"Missing ftppath_genbank in NCBI Assembly JSON"
|
49
|
+
)
|
50
|
+
end
|
51
|
+
|
46
52
|
url = "#{url_dir}/#{File.basename url_dir}_genomic.fna.gz"
|
47
53
|
download(
|
48
54
|
:web, :assembly_gz, url,
|
data/lib/miga/version.rb
CHANGED
@@ -12,7 +12,7 @@ module MiGA
|
|
12
12
|
# - String indicating release status:
|
13
13
|
# - rc* release candidate, not released as gem
|
14
14
|
# - [0-9]+ stable release, released as gem
|
15
|
-
VERSION = [1.2,
|
15
|
+
VERSION = [1.2, 15, 1].freeze
|
16
16
|
|
17
17
|
##
|
18
18
|
# Nickname for the current major.minor version.
|
@@ -20,7 +20,7 @@ module MiGA
|
|
20
20
|
|
21
21
|
##
|
22
22
|
# Date of the current gem relese.
|
23
|
-
VERSION_DATE = Date.new(2023, 1,
|
23
|
+
VERSION_DATE = Date.new(2023, 1, 24)
|
24
24
|
|
25
25
|
##
|
26
26
|
# References of MiGA
|
data/test/remote_dataset_test.rb
CHANGED
@@ -106,6 +106,12 @@ class RemoteDatasetTest < Test::Unit::TestCase
|
|
106
106
|
assert { rd.get_metadata[:is_ref_type] }
|
107
107
|
end
|
108
108
|
|
109
|
+
def test_missing_data
|
110
|
+
declare_remote_access
|
111
|
+
rd = MiGA::RemoteDataset.new('GCA_000484975.1', :assembly, :ncbi)
|
112
|
+
assert_raise(MiGA::RemoteDataMissingError) { rd.save_to(project, 'bad') }
|
113
|
+
end
|
114
|
+
|
109
115
|
# This test is too expensive (too much time to run it!)
|
110
116
|
# def test_net_timeout
|
111
117
|
# declare_remote_access
|
metadata
CHANGED
@@ -1,14 +1,14 @@
|
|
1
1
|
--- !ruby/object:Gem::Specification
|
2
2
|
name: miga-base
|
3
3
|
version: !ruby/object:Gem::Version
|
4
|
-
version: 1.2.
|
4
|
+
version: 1.2.15.1
|
5
5
|
platform: ruby
|
6
6
|
authors:
|
7
7
|
- Luis M. Rodriguez-R
|
8
8
|
autorequire:
|
9
9
|
bindir: bin
|
10
10
|
cert_chain: []
|
11
|
-
date: 2023-01-
|
11
|
+
date: 2023-01-24 00:00:00.000000000 Z
|
12
12
|
dependencies:
|
13
13
|
- !ruby/object:Gem::Dependency
|
14
14
|
name: daemons
|
@@ -141,6 +141,9 @@ files:
|
|
141
141
|
- lib/miga/cli/action/derep_wf.rb
|
142
142
|
- lib/miga/cli/action/doctor.rb
|
143
143
|
- lib/miga/cli/action/doctor/base.rb
|
144
|
+
- lib/miga/cli/action/doctor/databases.rb
|
145
|
+
- lib/miga/cli/action/doctor/distances.rb
|
146
|
+
- lib/miga/cli/action/doctor/operations.rb
|
144
147
|
- lib/miga/cli/action/download/base.rb
|
145
148
|
- lib/miga/cli/action/download/gtdb.rb
|
146
149
|
- lib/miga/cli/action/download/ncbi.rb
|
@@ -196,6 +199,8 @@ files:
|
|
196
199
|
- lib/miga/dataset/base.rb
|
197
200
|
- lib/miga/dataset/hooks.rb
|
198
201
|
- lib/miga/dataset/result.rb
|
202
|
+
- lib/miga/dataset/result/add.rb
|
203
|
+
- lib/miga/dataset/result/ignore.rb
|
199
204
|
- lib/miga/dataset/status.rb
|
200
205
|
- lib/miga/json.rb
|
201
206
|
- lib/miga/lair.rb
|