miga-base 0.7.26.1 → 1.0.2.0
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- checksums.yaml +4 -4
- data/lib/miga/_data/aai-intax.blast.tsv.gz +0 -0
- data/lib/miga/_data/aai-intax.diamond.tsv.gz +0 -0
- data/lib/miga/_data/aai-novel.blast.tsv.gz +0 -0
- data/lib/miga/_data/aai-novel.diamond.tsv.gz +0 -0
- data/lib/miga/cli/action/classify_wf.rb +2 -2
- data/lib/miga/cli/action/derep_wf.rb +1 -1
- data/lib/miga/cli/action/doctor.rb +57 -14
- data/lib/miga/cli/action/doctor/base.rb +47 -23
- data/lib/miga/cli/action/init.rb +11 -7
- data/lib/miga/cli/action/init/files_helper.rb +1 -0
- data/lib/miga/cli/action/ncbi_get.rb +3 -3
- data/lib/miga/cli/action/tax_dist.rb +2 -2
- data/lib/miga/cli/action/wf.rb +5 -4
- data/lib/miga/common.rb +1 -0
- data/lib/miga/daemon.rb +11 -4
- data/lib/miga/dataset/result.rb +10 -6
- data/lib/miga/json.rb +5 -4
- data/lib/miga/metadata.rb +5 -1
- data/lib/miga/parallel.rb +36 -0
- data/lib/miga/project.rb +8 -8
- data/lib/miga/project/base.rb +4 -4
- data/lib/miga/project/result.rb +2 -2
- data/lib/miga/sqlite.rb +10 -2
- data/lib/miga/version.rb +23 -9
- data/scripts/aai_distances.bash +16 -18
- data/scripts/ani_distances.bash +16 -17
- data/scripts/assembly.bash +31 -16
- data/scripts/haai_distances.bash +3 -27
- data/scripts/miga.bash +6 -4
- data/scripts/p.bash +1 -1
- data/scripts/read_quality.bash +9 -18
- data/scripts/trimmed_fasta.bash +14 -30
- data/scripts/trimmed_reads.bash +36 -36
- data/test/parallel_test.rb +31 -0
- data/test/project_test.rb +2 -1
- data/test/remote_dataset_test.rb +1 -1
- data/utils/distance/commands.rb +1 -0
- data/utils/distance/database.rb +0 -1
- data/utils/distance/runner.rb +2 -4
- data/utils/enveomics/Manifest/Tasks/fasta.json +39 -3
- data/utils/enveomics/Manifest/Tasks/fastq.json +50 -2
- data/utils/enveomics/Manifest/Tasks/mapping.json +70 -0
- data/utils/enveomics/Manifest/Tasks/other.json +77 -0
- data/utils/enveomics/Manifest/Tasks/sequence-identity.json +138 -1
- data/utils/enveomics/Manifest/categories.json +13 -4
- data/utils/enveomics/Scripts/Aln.cat.rb +206 -148
- data/utils/enveomics/Scripts/FastA.N50.pl +33 -29
- data/utils/enveomics/Scripts/FastA.fragment.rb +69 -61
- data/utils/enveomics/Scripts/FastA.sample.rb +61 -46
- data/utils/enveomics/Scripts/FastA.toFastQ.rb +69 -0
- data/utils/enveomics/Scripts/FastQ.maskQual.rb +89 -0
- data/utils/enveomics/Scripts/FastQ.tag.rb +59 -52
- data/utils/enveomics/Scripts/SRA.download.bash +6 -8
- data/utils/enveomics/Scripts/Table.prefScore.R +60 -0
- data/utils/enveomics/Scripts/aai.rb +3 -2
- data/utils/enveomics/Scripts/anir.rb +137 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/anir.rb +293 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/bm_set.rb +175 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/enveomics.rb +17 -17
- data/utils/enveomics/Scripts/lib/enveomics_rb/errors.rb +17 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/gmm_em.rb +30 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/match.rb +63 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/rbm.rb +49 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/stats.rb +3 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/stats/rand.rb +31 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/stats/sample.rb +152 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/utils.rb +73 -0
- data/utils/enveomics/Scripts/rbm-legacy.rb +172 -0
- data/utils/enveomics/Scripts/rbm.rb +87 -133
- data/utils/enveomics/Scripts/sam.filter.rb +148 -0
- data/utils/enveomics/enveomics.R/DESCRIPTION +2 -2
- data/utils/enveomics/enveomics.R/NAMESPACE +1 -1
- data/utils/enveomics/enveomics.R/R/prefscore.R +79 -0
- data/utils/enveomics/enveomics.R/R/utils.R +30 -0
- data/utils/enveomics/enveomics.R/README.md +1 -0
- data/utils/enveomics/enveomics.R/man/cash-enve.GrowthCurve-method.Rd +0 -1
- data/utils/enveomics/enveomics.R/man/cash-enve.RecPlot2-method.Rd +0 -1
- data/utils/enveomics/enveomics.R/man/cash-enve.RecPlot2.Peak-method.Rd +0 -1
- data/utils/enveomics/enveomics.R/man/enve.__tribs.Rd +10 -2
- data/utils/enveomics/enveomics.R/man/enve.barplot.Rd +16 -4
- data/utils/enveomics/enveomics.R/man/enve.cliopts.Rd +13 -3
- data/utils/enveomics/enveomics.R/man/enve.df2dist.Rd +8 -2
- data/utils/enveomics/enveomics.R/man/enve.df2dist.group.Rd +8 -2
- data/utils/enveomics/enveomics.R/man/enve.df2dist.list.Rd +9 -2
- data/utils/enveomics/enveomics.R/man/enve.growthcurve.Rd +13 -5
- data/utils/enveomics/enveomics.R/man/enve.prefscore.Rd +50 -0
- data/utils/enveomics/enveomics.R/man/enve.prune.dist.Rd +9 -2
- data/utils/enveomics/enveomics.R/man/enve.recplot.Rd +23 -6
- data/utils/enveomics/enveomics.R/man/enve.recplot2.Rd +13 -4
- data/utils/enveomics/enveomics.R/man/enve.recplot2.compareIdentities.Rd +8 -2
- data/utils/enveomics/enveomics.R/man/enve.recplot2.extractWindows.Rd +7 -2
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__mow_one.Rd +14 -3
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.em.Rd +10 -2
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.emauto.Rd +8 -2
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.mower.Rd +17 -9
- data/utils/enveomics/enveomics.R/man/enve.recplot2.windowDepthThreshold.Rd +6 -2
- data/utils/enveomics/enveomics.R/man/enve.selvector.Rd +23 -0
- data/utils/enveomics/enveomics.R/man/enve.tribs.Rd +14 -5
- data/utils/enveomics/enveomics.R/man/plot.enve.GrowthCurve.Rd +19 -4
- data/utils/enveomics/enveomics.R/man/plot.enve.TRIBS.Rd +11 -3
- data/utils/enveomics/enveomics.R/man/plot.enve.TRIBStest.Rd +11 -4
- data/utils/enveomics/enveomics.R/man/plot.enve.recplot2.Rd +26 -12
- data/utils/multitrim/Multitrim How-To.pdf +0 -0
- data/utils/multitrim/README.md +67 -0
- data/utils/multitrim/multitrim.py +1555 -0
- data/utils/multitrim/multitrim.yml +13 -0
- data/utils/requirements.txt +4 -3
- data/utils/subclade/pipeline.rb +2 -2
- metadata +35 -7
- data/utils/enveomics/Scripts/lib/enveomics_rb/stat.rb +0 -30
@@ -1,5 +1,5 @@
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Package: enveomics.R
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Version: 1.
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Version: 1.8.0
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Authors@R: c(person("Luis M.","Rodriguez-R",role=c("aut","cre"),
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email="lmrodriguezr@gmail.com"))
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Title: Various Utilities for Microbial Genomics and Metagenomics
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License: Artistic-2.0
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LazyData: yes
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Encoding: UTF-8
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RoxygenNote:
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RoxygenNote: 7.0.2
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@@ -36,4 +36,4 @@ export(
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enve.recplot2.compareIdentities,
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enve.recplot2.coordinates, enve.recplot2.seqdepth, enve.recplot2.ANIr,
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enve.growthcurve, plot.enve.GrowthCurve, summary.enve.GrowthCurve,
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enve.col2alpha, enve.truncate)
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enve.col2alpha, enve.truncate, enve.selvector, enve.prefscore)
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#' Enveomics: Pref Score
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#'
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#' Estimate preference score of species based on occupancy in biased sample sets
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#'
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#' @param x
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#' Occupancy matrix (logical or numeric binary) with species as rows and samples
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#' as columns
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#' @param set
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#' Vector indicating samples in the test set. It can be any selection vector:
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#' boolean (same length as the number of columns in \code{x}), or numeric or
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#' character vector with indexes of the \code{x} columns.
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#' @param ignore
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#' Vector indicating species to ignore. It can be any selection vector with
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#' respect to the rows in \code{x} (see \code{set}).
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#' @param signif.thr Absolute value of the significance threshold
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#' @param plot Indicates if a plot should be generated
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#' @param col.above Color for points significantly above zero
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#' @param col.equal Color for points not significantly different from zero
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#' @param col.below Color for points significantly below zero
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#' @param ... Any additional parameters supported by \code{plot}
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#'
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#' @return Returns a named vector of preference scores.
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#'
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#' @author Luis M. Rodriguez-R [aut, cre]
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#'
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#' @export
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enve.prefscore <- function
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(
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x,
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set,
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ignore = NULL,
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signif.thr,
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plot = TRUE,
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col.above = rgb(148, 17, 0, maxColorValue = 255),
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col.equal = rgb(189, 189, 189, maxColorValue = 255),
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col.below = rgb(47, 84, 150, maxColorValue = 255),
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...
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) {
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# Normalize classes and filter universe
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x <- !!as.matrix(x)
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if(is.null(colnames(x))) colnames(x) <- 1:ncol(x)
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if(is.null(rownames(x))) rownames(x) <- 1:nrow(x)
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set <- enve.selvector(set, colnames(x))
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universe <- !enve.selvector(ignore, rownames(x))
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x.u <- x[universe, ]
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if(missing(signif.thr)) signif.thr <- 1 + 100 / length(universe)
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# Base (null) probabilities
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p_a <- (rowSums(x.u) + 1) / (ncol(x.u) + 2)
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p_b <- (colSums(x.u) + 1) / (nrow(x.u) + 2)
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p_p <- p_a %*% t(p_b)
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# Set preference score
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expected <- (rowSums(p_p[, set]) - rowSums(p_p[, !set])) / sum(p_p)
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observed <- (rowSums(x.u[, set]) - rowSums(x.u[, !set])) / sum(x.u)
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y <- observed / abs(expected)
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names(y) <- rownames(x)[universe]
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y.code <- cut(y, c(-Inf, -signif.thr, signif.thr, Inf), 1:3)
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# Plot
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if(plot) {
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idx <- (1:nrow(x))[universe]
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opts.def <- list(x = idx, y = y, ylim = c(-1, 1) * max(abs(y)),
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xlab = 'Species', ylab = 'Preference score', xlim = c(0, nrow(x)+1),
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col = c(col.above, col.equal, col.below)[y.code],
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las = 1, xaxs = 'i', pch = 15)
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opts <- list(...)
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for(i in names(opts.def)) if(is.null(opts[[i]])) opts[[i]] <- opts.def[[i]]
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do.call('plot', opts)
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abline(h = 0, lty = 1, col = rgb(0, 0, 0, 1/4))
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abline(h = c(-1, 1) * signif.thr, lty = 2, col = rgb(0, 0, 0, 1/4))
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}
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# Print and return
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print(table(c(c('<', '=', '>')[y.code], rep('Tot', length(y.code)))))
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cat('---------\n')
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return(y)
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}
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y <- sort(x)[ -c(seq(1, n), seq(length(x)+1-n, length(x))) ]
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return(FUN(y))
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}
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#' Enveomics: Selection vector
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#'
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#' Normalizes a selection vector \code{sel} to a logical vector with indexes
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#' from \code{dim.names}.
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#'
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#' @param sel A vector of numbers, characters, or booleans.
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#' @param dim.names A vector of names from which to select.
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#'
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#' @return Returns a logical vector with the same length as \code{dim.name}.
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#'
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#' @author Luis M. Rodriguez-R [aut, cre]
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#'
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#' @export
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enve.selvector <- function(sel, dim.names) {
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if(is.logical(sel)) {
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if(length(sel) != length(dim.names))
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stop('sel is logical but differs in length from dim.names')
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sel
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} else if(is.numeric(sel)) {
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if(max(sel) > length(dim.names))
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stop('sel includes numeric index beyond the length of dim.names')
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1:length(dim.names) %in% sel
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} else {
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if(any(!sel %in% dim.names))
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stop('sel includes character index missing from dim.names')
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dim.names %in% sel
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}
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}
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[Recruitment plots working document](https://github.com/lmrodriguezr/enveomics/blob/master/Docs/recplot2.md).
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## Changelog
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* 1.8.0: New functions `enve.selvector` and `enve.prefscore`.
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* 1.7.1: Improved efficiency of `enve.df2dist` about five-fold.
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* 1.7.0: Uniformized output for `enve.recplot2.extractWindows` and
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`enve.recplot2.coordinates` to ease automation. Thanks to Tomeu Viver and
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\alias{enve.__tribs}
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\title{Enveomics: TRIBS - Internal Ancillary Function}
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\usage{
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enve.__tribs(
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enve.__tribs(
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rep,
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frx,
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selection,
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dimensions,
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dots,
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dist.method,
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summary.fx,
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dist
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)
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}
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\arguments{
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\item{rep}{Replicates}
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\alias{enve.barplot}
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\title{Enveomics: Barplot}
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\usage{
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top = 25,
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colors.per.group = 9,
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bars.width = 4,
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legend.ncol = 1,
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other.col = "#000000",
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add.trend = FALSE,
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organic.trend = FALSE,
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sort.by = median,
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min.report = 101,
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order = NULL,
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col,
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\arguments{
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\item{x}{Can be either the input data or the path to the file containing
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\title{Enveomics: Cliopts}
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\usage{
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rd_file,
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positional_arguments,
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usage,
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mandatory = c(),
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vectorize = c(),
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ignore = c(),
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number = c(),
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defaults = list(),
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o_desc = list(),
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p_desc = ""
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)
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}
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\item{fx}{Function for which the interface should be generated.}
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\alias{enve.df2dist}
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\title{Enveomics: Data Frame to Dist}
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\usage{
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dist.index = 3,
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default.d = NA,
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max.sim = 0
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)
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\title{Enveomics: Data Frame to Dist (Group)}
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\usage{
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enve.df2dist.group(
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x,
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+
obj1.index = 1,
|
10
|
+
obj2.index = 2,
|
11
|
+
dist.index = 3,
|
12
|
+
summary = median,
|
13
|
+
empty.rm = TRUE
|
14
|
+
)
|
9
15
|
}
|
10
16
|
\arguments{
|
11
17
|
\item{x}{A dataframe (or coercible object) with at least three columns:
|
@@ -4,8 +4,15 @@
|
|
4
4
|
\alias{enve.df2dist.list}
|
5
5
|
\title{Enveomics: Data Frame to Dist (List)}
|
6
6
|
\usage{
|
7
|
-
enve.df2dist.list(
|
8
|
-
|
7
|
+
enve.df2dist.list(
|
8
|
+
x,
|
9
|
+
groups,
|
10
|
+
obj1.index = 1,
|
11
|
+
obj2.index = 2,
|
12
|
+
dist.index = 3,
|
13
|
+
empty.rm = TRUE,
|
14
|
+
...
|
15
|
+
)
|
9
16
|
}
|
10
17
|
\arguments{
|
11
18
|
\item{x}{A dataframe (or coercible object) with at least three columns:
|
@@ -4,11 +4,19 @@
|
|
4
4
|
\alias{enve.growthcurve}
|
5
5
|
\title{Enveomics: Growth Curve}
|
6
6
|
\usage{
|
7
|
-
enve.growthcurve(
|
8
|
-
|
9
|
-
|
10
|
-
|
11
|
-
|
7
|
+
enve.growthcurve(
|
8
|
+
x,
|
9
|
+
times = 1:nrow(x),
|
10
|
+
triplicates = FALSE,
|
11
|
+
design,
|
12
|
+
new.times = seq(min(times), max(times), length.out = length(times) * 10),
|
13
|
+
level = 0.95,
|
14
|
+
interval = c("confidence", "prediction"),
|
15
|
+
plot = TRUE,
|
16
|
+
FUN = function(t, K, r, P0) K * P0 * exp(r * t)/(K + P0 * (exp(r * t) - 1)),
|
17
|
+
nls.opt = list(),
|
18
|
+
...
|
19
|
+
)
|
12
20
|
}
|
13
21
|
\arguments{
|
14
22
|
\item{x}{Data frame (or coercible) containing the observed growth data
|
@@ -0,0 +1,50 @@
|
|
1
|
+
% Generated by roxygen2: do not edit by hand
|
2
|
+
% Please edit documentation in R/prefscore.R
|
3
|
+
\name{enve.prefscore}
|
4
|
+
\alias{enve.prefscore}
|
5
|
+
\title{Enveomics: Pref Score}
|
6
|
+
\usage{
|
7
|
+
enve.prefscore(
|
8
|
+
x,
|
9
|
+
set,
|
10
|
+
ignore = NULL,
|
11
|
+
signif.thr,
|
12
|
+
plot = TRUE,
|
13
|
+
col.above = rgb(148, 17, 0, maxColorValue = 255),
|
14
|
+
col.equal = rgb(189, 189, 189, maxColorValue = 255),
|
15
|
+
col.below = rgb(47, 84, 150, maxColorValue = 255),
|
16
|
+
...
|
17
|
+
)
|
18
|
+
}
|
19
|
+
\arguments{
|
20
|
+
\item{x}{Occupancy matrix (logical or numeric binary) with species as rows and samples
|
21
|
+
as columns}
|
22
|
+
|
23
|
+
\item{set}{Vector indicating samples in the test set. It can be any selection vector:
|
24
|
+
boolean (same length as the number of columns in \code{x}), or numeric or
|
25
|
+
character vector with indexes of the \code{x} columns.}
|
26
|
+
|
27
|
+
\item{ignore}{Vector indicating species to ignore. It can be any selection vector with
|
28
|
+
respect to the rows in \code{x} (see \code{set}).}
|
29
|
+
|
30
|
+
\item{signif.thr}{Absolute value of the significance threshold}
|
31
|
+
|
32
|
+
\item{plot}{Indicates if a plot should be generated}
|
33
|
+
|
34
|
+
\item{col.above}{Color for points significantly above zero}
|
35
|
+
|
36
|
+
\item{col.equal}{Color for points not significantly different from zero}
|
37
|
+
|
38
|
+
\item{col.below}{Color for points significantly below zero}
|
39
|
+
|
40
|
+
\item{...}{Any additional parameters supported by \code{plot}}
|
41
|
+
}
|
42
|
+
\value{
|
43
|
+
Returns a named vector of preference scores.
|
44
|
+
}
|
45
|
+
\description{
|
46
|
+
Estimate preference score of species based on occupancy in biased sample sets
|
47
|
+
}
|
48
|
+
\author{
|
49
|
+
Luis M. Rodriguez-R [aut, cre]
|
50
|
+
}
|
@@ -4,8 +4,15 @@
|
|
4
4
|
\alias{enve.prune.dist}
|
5
5
|
\title{Enveomics: Prune Dist}
|
6
6
|
\usage{
|
7
|
-
enve.prune.dist(
|
8
|
-
|
7
|
+
enve.prune.dist(
|
8
|
+
t,
|
9
|
+
dist.quantile = 0.25,
|
10
|
+
min_dist,
|
11
|
+
quiet = FALSE,
|
12
|
+
max_iters = 100,
|
13
|
+
min_nodes_random = 40000,
|
14
|
+
random_nodes_frx = 1
|
15
|
+
)
|
9
16
|
}
|
10
17
|
\arguments{
|
11
18
|
\item{t}{A \strong{phylo} object or a path to the Newick file.}
|
@@ -4,12 +4,29 @@
|
|
4
4
|
\alias{enve.recplot}
|
5
5
|
\title{Enveomics: Recruitment Plots}
|
6
6
|
\usage{
|
7
|
-
enve.recplot(
|
8
|
-
|
9
|
-
|
10
|
-
|
11
|
-
|
12
|
-
|
7
|
+
enve.recplot(
|
8
|
+
prefix,
|
9
|
+
id.min = NULL,
|
10
|
+
id.max = NULL,
|
11
|
+
id.binsize = NULL,
|
12
|
+
id.splines = 0,
|
13
|
+
id.metric = "id",
|
14
|
+
id.summary = "sum",
|
15
|
+
pos.min = 1,
|
16
|
+
pos.max = NULL,
|
17
|
+
pos.binsize = 1000,
|
18
|
+
pos.splines = 0,
|
19
|
+
rec.col1 = "white",
|
20
|
+
rec.col2 = "black",
|
21
|
+
main = NULL,
|
22
|
+
contig.col = grey(0.85),
|
23
|
+
ret.recplot = FALSE,
|
24
|
+
ret.hist = FALSE,
|
25
|
+
ret.mode = FALSE,
|
26
|
+
id.cutoff = NULL,
|
27
|
+
verbose = TRUE,
|
28
|
+
...
|
29
|
+
)
|
13
30
|
}
|
14
31
|
\arguments{
|
15
32
|
\item{prefix}{Path to the prefix of the BlastTab.catsbj.pl output files. At
|
@@ -4,11 +4,20 @@
|
|
4
4
|
\alias{enve.recplot2}
|
5
5
|
\title{Enveomics: Recruitment Plot (2)}
|
6
6
|
\usage{
|
7
|
-
enve.recplot2(
|
8
|
-
|
7
|
+
enve.recplot2(
|
8
|
+
prefix,
|
9
|
+
plot = TRUE,
|
10
|
+
pos.breaks = 1000,
|
11
|
+
pos.breaks.tsv = NA,
|
12
|
+
id.breaks = 60,
|
13
|
+
id.free.range = FALSE,
|
9
14
|
id.metric = c("identity", "corrected identity", "bit score"),
|
10
|
-
id.summary = sum,
|
11
|
-
|
15
|
+
id.summary = sum,
|
16
|
+
id.cutoff = 95,
|
17
|
+
threads = 2,
|
18
|
+
verbose = TRUE,
|
19
|
+
...
|
20
|
+
)
|
12
21
|
}
|
13
22
|
\arguments{
|
14
23
|
\item{prefix}{Path to the prefix of the \code{BlastTab.catsbj.pl} output files. At
|