miga-base 0.7.25.2 → 0.7.25.3

data/utils/enveomics/Scripts/EBIseq2tax.rb

@@ -1,83 +0,0 @@
-#!/usr/bin/env ruby
-
-#
-# @author  Luis M. Rodriguez-R <lmrodriguezr at gmail dot com>
-# @update  Oct-13-2015
-# @license Artistic License 2.0
-#
-
-$:.push File.expand_path(File.dirname(__FILE__) + "/lib")
-require "enveomics_rb/remote_data"
-use "nokogiri"
-
-#================================[ Options parsing ]
-$o = {
-   q: false, ids: [], dbfrom: "uniprotkb", header: true,
-   ret: "ScientificName",
-   ranks: %w(superkingdom phylum class order family genus species)}
-
-OptionParser.new do |opt|
-   opt.banner = "
-   Maps a list of EBI-supported IDs to their corresponding NCBI taxonomy using
-   EBI RESTful API. Avoid using this script on millions of entries at a time,
-   since each entry elicits requests to EBI and NCBI servers.
-
-   Usage: #{$0} [options]".gsub(/^ +/,"")
-   opt.separator ""
-   opt.on("-i", "--ids ID1,ID2,...", Array,
-      "Comma-separated list of EBI IDs. Required unless -I is passed."
-      ){ |v| $o[:ids]=v }
-   opt.on("-I", "--infile FILE",
-      "Raw text file containing the list of EBI IDs, one per line.",
-      "Required unless -i is passed."){ |v| $o[:infile]=v }
-   opt.on("-d", "--database DB",
-      "EBI database defining the EBI IDs. By default: " + $o[:dbfrom].to_s + "."
-      ){ |v| $o[:dbfrom]=v }
-   opt.on("-r", "--ranks RANK1,RANK2,...", Array,
-      "Taxonomic ranks to report. By default:",
-      $o[:ranks].join(",") + "."){ |v| $o[:ranks]=v }
-   opt.on("-n", "--noheader",
-      "Do not includ a header in the output."){ $o[:header]=false }
-   opt.on("-t", "--taxids",
-      "Return Taxonomy IDs instead of scientific names."){ $o[:ret]="TaxId" }
-   opt.on("-q", "--quiet", "Run quietly."){ |v| $o[:q]=true }
-   opt.on("-h", "--help","Display this screen") do
-      puts opt
-      exit
-   end
-   opt.separator ""
-end.parse!
-
-#================================[ Main ]
-begin
-   $o[:ids] += File.readlines($o[:infile]).map{ |l| l.chomp } unless
-      $o[:infile].nil?
-   $o[:ranks].map!{ |r| r.downcase }
-   puts (["ID", "TaxId"] + $o[:ranks].map{ |r| r.capitalize }).join("\t") if
-      $o[:header]
-   $o[:ids].each do |id|
-      id = $1 if id =~ /^[a-z]+\|\S+\|(\S+)/
-      taxid = RemoteData.ebiseq2taxid(id, $o[:dbfrom])
-      if taxid.nil?
-	 warn "Cannot find link to taxonomy: #{id}"
-	 next
-      end
-      taxonomy = {}
-      unless taxid.nil?
-	 doc = Nokogiri::XML( RemoteData.efetch({db: "taxonomy", id: taxid}) )
-	 taxonomy[ doc.at_xpath("/TaxaSet/Taxon/Rank").content ] =
-	    doc.at_xpath("/TaxaSet/Taxon/#{$o[:ret]}").content
-	 doc.xpath("/TaxaSet/Taxon/LineageEx/Taxon").each do |taxon|
-	    taxonomy[ taxon.at_xpath("./Rank").content ] =
-	       taxon.at_xpath("./#{$o[:ret]}").content
-	 end
-      end
-      puts ([id, taxid] +
-	 $o[:ranks].map{ |rank| taxonomy[ rank ] ||= "" }).join("\t")
-   end # $o[:ids].each
-rescue => err
-   $stderr.puts "Exception: #{err}\n\n"
-   err.backtrace.each { |l| $stderr.puts l + "\n" }
-   err
-end
-

data/utils/enveomics/Scripts/FastA.N50.pl

@@ -1,56 +0,0 @@
-#!/usr/bin/env perl
-#
-# @author: Luis M. Rodriguez-R <lmrodriguezr at gmail dot com>
-# @update: Oct 07 2015
-# @license: artistic license 2.0
-#
-use strict;
-use warnings;
-use List::Util qw/sum min max/;
-
-my ($seqs, $minlen, $n__) = @ARGV;
-$seqs or die "
-Description:
-   Calculates the N50 value of a set of sequences.  Alternatively, it
-   can calculate other N** values.  It also calculates the total number
-   of sequences and the total added length.
-   
-Usage:
-   $0 seqs.fa[ minlen[ **]]
-
-   seqs.fa	A FastA file containing the sequences.
-   minlen	(optional) The minimum length to take into consideration.
-   		By default: 0.
-   **		Value N** to calculate.  By default: 50 (N50).
-";
-$minlen ||= 0;
-$n__    ||= 50;
-
-my @len = ();
-open SEQ, "<", $seqs or die "Cannot open file: $seqs: $!\n";
-while(<SEQ>){
-   if(/^>/){
-      push @len, 0;
-   }else{
-      next if /^;/;
-      chomp;
-      s/\W//g;
-      $len[-1]+=length $_;
-   }
-}
-close SEQ;
-@len = sort { $a <=> $b } map { $_>=$minlen?$_:() } @len;
-my $tot = (sum(@len) || 0);
-
-my $thr = $n__*$tot/100;
-my $pos = 0;
-for(@len){
-   $pos+= $_;
-   if($pos>=$thr){
-      print "N$n__: $_\n";
-      last;
-   }
-}
-print "Sequences: ".scalar(@len)."\n";
-print "Total length: $tot\n";
-

data/utils/enveomics/Scripts/FastA.extract.rb

@@ -1,152 +0,0 @@
-#!/usr/bin/env ruby
-
-#
-# @author Luis M. Rodriguez-R <lmrodriguezr at gmail dot com>
-# @license Artistic-2.0
-#
-
-require 'optparse'
-
-o = {q: false}
-ARGV << '-h' if ARGV.size==0
-
-OptionParser.new do |opt|
-  opt.banner = "
-Extracts a list of sequences and/or coordinates from multi-FastA files.
-
-Usage: #{$0} [options]"
-  opt.separator ''
-  opt.separator 'Mandatory'
-  opt.on('-i', '--in PATH', 'Input FastA file.'){ |v| o[:i] = v }
-  opt.on('-o', '--out PATH', 'Output FastA file.'){ |v| o[:o] = v }
-  opt.on('-c', '--coords STRING',
-    'Comma-delimited list of coordinates (mandatory unless -C is passed).',
-    'The format of the coordinates is "SEQ:FROM..TO" or "SEQ:FROM~LEN":',
-    'SEQ: Sequence ID, or * (asterisk) to extract range from all sequences',
-    'FROM: Integer, position of the first base to include (can be negative)',
-    'TO: Integer, last base to include (can be negative)',
-    'LEN: Length of the range to extract'
-    ){ |v| o[:c] = v }
-  opt.separator ''
-  opt.separator 'Options'
-  opt.on('-C', '--coords-file PATH',
-    'File containing the coordinates, one per line.',
-    'Each line must follow the format described for -c.'){ |v| o[:C] = v }
-  opt.on('-q', '--quiet', 'Run quietly (no STDERR output).'){ o[:q] = true }
-  opt.on('-h', '--help', 'Display this screen.') do
-    puts opt
-    exit
-  end
-  opt.separator ''
-end.parse!
-abort '-i is mandatory.' if o[:i].nil?
-abort '-o is mandatory.' if o[:o].nil?
-abort '-c is mandatory.' if o[:c].nil? and o[:C].nil?
-
-# Classses to parse coordinates
-class SeqCoords
-  attr :id, :from, :to, :length, :str
-  def initialize(str)
-    @str = str
-    m = /(\S+):(-?\d+)(~|\.\.)(-?\d+)/.match str
-    raise "Cannot parse coordinates: #{str}" if m.nil?
-    @id = m[1]
-    @from = m[2].to_i
-    if m[3] == '~'
-      @length = m[4].to_i
-    else
-      @to = m[4].to_i
-    end
-  end
-
-  def extract(id, seq)
-    return nil unless concerns? id
-    from_i = from > 0 ? from : seq.length + 1 + from
-    if to.nil?
-      seq[from_i, length]
-    else
-      to_i = to > 0 ? to : seq.length + 1 + to
-      seq[from_i .. to_i]
-    end
-  end
-
-  def concerns?(seq_id)
-    return true if id == '*'
-    return id == seq_id
-  end
-end
-
-class SeqCoordsCollection
-  class << self
-    def from_str(str)
-      c = new
-      str.split(',').each { |i| c << SeqCoords.new(i) }
-      c
-    end
-    def from_file(path)
-      c = new
-      File.open(path, 'r') do |fh|
-        fh.each{ |i| c << SeqCoords.new(i.chomp) }
-      end
-      c
-    end
-  end
-
-  attr :collection
-
-  def initialize
-    @collection = []
-  end
-
-  def <<(coords)
-    @collection << coords
-  end
-
-  def extract(id, seq)
-    @collection.map{ |c| c.extract(id, seq) }.compact
-  end
-end
-
-# Functions to parse sequences
-def do_stuff(id, sq)
-  return if id.nil? or sq.empty?
-  @n_in += 1
-  sq.gsub!(/[^A-Za-z]/, '')
-  i = 0
-  @coll.extract(id, sq).each do |new_sq|
-    @ofh.puts ">#{id}:#{i += 1}"
-    @ofh.puts new_sq
-    @n_out += 1
-  end
-end
-
-# Parse coordinates
-$stderr.puts 'Parsing coordinates' unless o[:q]
-@coll = o[:c].nil? ? SeqCoordsCollection.from_file(o[:C]) :
-  SeqCoordsCollection.from_str(o[:c])
-$stderr.puts "  Coordinates found: #{@coll.collection.size}"
-
-# Parse sequences
-$stderr.puts 'Parsing sequences' unless o[:q]
-@n_in = 0
-@n_out = 0
-@ofh = File.open(o[:o], 'w')
-File.open(o[:i], 'r') do |fh|
-  id = nil
-  sq = ''
-  fh.each do |ln|
-    next if ln =~ /^;/
-    if ln =~ /^>(\S+)/
-      id = $1
-      do_stuff(id, sq)
-      sq = ''
-    else
-      sq << ln
-    end
-  end
-  do_stuff(id, sq)
-end
-@ofh.close
-$stderr.puts "  Input sequences: #{@n_in}"
-$stderr.puts "  Output fragments: #{@n_out}"
-

data/utils/enveomics/Scripts/FastA.filter.pl

@@ -1,52 +0,0 @@
-#!/usr/bin/env perl
-#
-# @author  Luis M. Rodriguez-R <lmrodriguezr at gmail dot com>
-# @update  Oct-07-2015
-# @license artistic license 2.0
-#
-
-use warnings;
-use strict;
-use Getopt::Std;
-
-sub HELP_MESSAGE { die "
-.Description:
-   Extracts a subset of sequences from a FastA file.
-
-.Usage: $0 [options] list.txt seqs.fa > subset.fa
-   
-   [options]
-   -r		Reverse list.  Extracts sequences NOT present in the list.
-   -q		Runs quietly.
-   -h		Prints this message and exits.
-
-   [mandatory]
-   list.txt	List of sequences to extract.
-   seqs.fa	FastA file containing the superset of sequences.
-   subset.fa	FastA file to be created.
-
-" }
-
-my %o=();
-getopts('rhq', \%o);
-my($list, $fa) = @ARGV;
-($list and $fa) or &HELP_MESSAGE;
-$o{h} and &HELP_MESSAGE;
-
-print STDERR "Reading list.\n" unless $o{q};
-open LI, "<", $list or die "Cannot read file: $list: $!\n";
-my %li = map { chomp; $_ => 1 } <LI>;
-close LI;
-
-print STDERR "Filtering FastA.\n" unless $o{q};
-open FA, "<", $fa or die "Cannot read file: $fa: $!\n";
-my $good = 0;
-while(my $ln = <FA>){
-   next if $ln =~ /^;/;
-   chomp $ln;
-   if($ln =~ m/^>((\S+).*)/){ $good = (exists $li{$1} or exists $li{">$1"} or exists $li{$2} or exists $li{$ln}) }
-   elsif($ln =~ m/^>/){ $good=$o{r}; print STDERR "Warning: Non-cannonical defline, line $.: $ln\n" }
-   print "$ln\n" if (($good and not $o{r}) or ($o{r} and not $good));
-}
-close FA;
-

data/utils/enveomics/Scripts/FastA.filterLen.pl

@@ -1,28 +0,0 @@
-#!/usr/bin/env perl
-
-use warnings;
-use strict;
-use Bio::SeqIO;
-
-my $file = $ARGV[0]; 
-my $min = $ARGV[1];
-($file and $min) or die <<HELP
-
-This script will filter a multi fastA file by length
-
-Usage "perl $0 fastafile minlenght "
-HELP
-;
-my $seq_in  = Bio::SeqIO->new( -format => 'fasta',-file => $file);
-
-while( my $seq1 = $seq_in->next_seq() ) {	
-	
-	my $id  = $seq1->primary_id;
-	chomp $id;
-	my $seq = $seq1->seq;
-	chomp $seq;
-	my $lseq = length($seq);
-	if($lseq>=$min){
-		print ">$id","\n",$seq,"\n";	
-	}
-}

data/utils/enveomics/Scripts/FastA.filterN.pl

@@ -1,60 +0,0 @@
-#!/usr/bin/env perl
-# 
-# @author  Luis M. Rodriguez-R
-# @update  Oct-07-2015
-# @license artistic license 2.0
-# 
-
-use warnings;
-use strict;
-
-my($file, $content, $stretch) = @ARGV;
-$file or die <<HELP
-
-Description:
-   Filter sequences by N-content and presence of long homopolymers.
-Usage:
-   $0 sequences.fa [content [stretch]] > filtered.fa
-Where:
-   sequences.fa	Input file in FastA format
-   content	A number between 0 and 1 indicating the maximum proportion of Ns
-   		(1 to turn off, 0.5 by default)
-   stretch	A number indicating the maximum number of consecutive identical
-   		nucleotides allowed (0 to turn off, 100 by default)
-   filtered.fa	Filtered set of sequences.
-
-HELP
-;
-($content ||= 0.5)+=0;
-($stretch ||= 100)+=0;
-
-my $good = 0;
-my $N = 0;
-
-FASTA: {
-   local $/ = "\n>";
-   open FILE, "<", $file or die "I can not open the file: $file: $!\n";
-   SEQ: while(<FILE>){
-      $N++;
-      s/^;.*//gm;
-      s/>//g;
-      my($n,$s) = split /\n/, $_, 2;
-      (my $clean = $s) =~ s/[^ACTGN]//g;
-      if($content < 1){
-         (my $Ns = $clean) =~ s/[^N]//g;
-	 next SEQ if length($Ns)>length($clean)*$content;
-      }
-      if($stretch > 0){
-         for my $nuc (qw(A C T G N)){
-	    next SEQ if $clean =~ m/[$nuc]{$stretch}/;
-	 }
-      }
-      print ">$n\n$s\n";
-      $good++;
-   }
-   close FILE;
-   print STDERR "Total sequences: $N\nAfter filtering: $good\n";
-}
-
-
-

data/utils/enveomics/Scripts/FastA.fragment.rb

@@ -1,92 +0,0 @@
-#!/usr/bin/env ruby
-
-# @author  Luis M. Rodriguez-R
-# @license artistic license 2.0
-
-$:.push File.expand_path("../lib", __FILE__)
-require "enveomics_rb/enveomics"
-require "enveomics_rb/stat"
-
-o = {q:false, completeness:nil, minlen:500, shuffle:true}
-OptionParser.new do |opts|
-  opts.banner = "
-Simulates incomplete (fragmented) drafts from complete genomes.
-
-Usage: #{$0} [options]"
-  opts.separator ""
-  opts.separator "Mandatory"
-  opts.on("-i", "--in FILE",
-    "Path to the FastA file containing the complete sequences."
-    ){ |v| o[:in] = v }
-  opts.on("-o", "--out FILE", "Path to the FastA to create."){ |v| o[:out] = v }
-  opts.on("-c", "--completeness FLOAT",
-    "Fraction of genome completeness to simulate from 0 to 1."
-    ){ |v| o[:completeness] = v.to_f }
-  opts.separator ""
-  opts.separator "Options"
-  opts.on("-m", "--minlen INT",
-    "Minimum fragment length to report. By default: #{o[:minlen]}."
-    ){ |v| o[:minlen] = v.to_i }
-  opts.on("-s", "--sorted", "Keep fragments sorted as in the input file. ",
-    "By default, fragments are shuffled."){ |v| o[:shuffle] = !v }
-  opts.on("-q", "--quiet", "Run quietly (no STDERR output)"){ o[:q] = true }
-  opts.on("-h", "--help", "Display this screen") do
-    puts opts
-    exit
-  end
-  opts.separator ""
-end.parse!
-abort "-i is mandatory" if o[:in].nil?
-abort "-o is mandatory" if o[:out].nil?
-abort "-c is mandatory" if o[:completeness].nil?
-
-begin
-  # Read input sequences
-  g_id  = []
-  g_seq = []
-  File.open(o[:in], "r") do |ifh|
-    id = ""
-    ifh.each_line do |ln|
-      if ln =~ /^>(\S*)/
-        g_id  << $1
-        g_seq << ""
-      else
-        g_seq[g_seq.size-1] += ln.gsub(/[^A-Za-z]/,"")
-      end
-    end
-  end
-  
-  # Fragment genomes
-  f = {}
-  binlen = [1, (o[:minlen].to_f/(1.5**2)).ceil].max
-  p = [0.001, [1.0, 1.0 - (o[:completeness]/1.25 + 0.1)].min].max
-  while not g_seq.empty?
-    id  = g_id.shift
-    seq = g_seq.shift
-    gL  = seq.length
-    while not seq.empty?
-      fL = [0, ((Enve::Stat.r_geom(p).to_f +
-                  Enve::Stat.r_unif(-0.5,0.5))*binlen).round].max
-      f["#{f.size+1}_#{id}"] = seq[0,fL] if fL >= o[:minlen]
-      seq = seq[(fL+1) .. -1]
-      seq = "" if seq.nil?
-    end
-  end
-
-  # Save output
-  k = f.keys
-  k.shuffle! if o[:shuffle]
-  File.open(o[:out], "w") do |ofh|
-    k.each do |id|
-      ofh.puts ">#{id}"
-      ofh.puts f[id].gsub(/(\S{50})/, "\\1\n")
-    end
-  end
-  
-rescue => err
-   $stderr.puts "Exception: #{err}\n\n"
-   err.backtrace.each { |l| $stderr.puts l + "\n" }
-   err
-end
-
-