miga-base 0.7.22.0 → 0.7.25.0
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- checksums.yaml +4 -4
- data/Gemfile +3 -0
- data/README.md +1 -1
- data/Rakefile +1 -0
- data/lib/miga/cli/action/add.rb +10 -8
- data/lib/miga/cli/action/classify_wf.rb +12 -11
- data/lib/miga/cli/action/derep_wf.rb +3 -9
- data/lib/miga/cli/action/edit.rb +0 -1
- data/lib/miga/cli/action/find.rb +1 -1
- data/lib/miga/cli/action/generic.rb +1 -1
- data/lib/miga/cli/action/get.rb +7 -2
- data/lib/miga/cli/action/get_db.rb +16 -21
- data/lib/miga/cli/action/index_wf.rb +4 -2
- data/lib/miga/cli/action/init.rb +93 -144
- data/lib/miga/cli/action/init/daemon_helper.rb +1 -2
- data/lib/miga/cli/action/init/files_helper.rb +119 -0
- data/lib/miga/cli/action/ncbi_get.rb +1 -1
- data/lib/miga/cli/action/new.rb +15 -9
- data/lib/miga/cli/action/option.rb +44 -0
- data/lib/miga/cli/action/preproc_wf.rb +7 -5
- data/lib/miga/cli/action/quality_wf.rb +3 -3
- data/lib/miga/cli/action/tax_dist.rb +1 -1
- data/lib/miga/cli/action/tax_test.rb +1 -1
- data/lib/miga/cli/action/wf.rb +71 -53
- data/lib/miga/cli/base.rb +17 -5
- data/lib/miga/cli/objects_helper.rb +23 -18
- data/lib/miga/common.rb +4 -2
- data/lib/miga/common/net.rb +74 -0
- data/lib/miga/common/with_option.rb +83 -0
- data/lib/miga/common/with_result.rb +3 -2
- data/lib/miga/dataset/base.rb +20 -2
- data/lib/miga/dataset/result.rb +5 -3
- data/lib/miga/metadata.rb +25 -13
- data/lib/miga/project/base.rb +82 -2
- data/lib/miga/project/result.rb +4 -4
- data/lib/miga/remote_dataset.rb +2 -0
- data/lib/miga/result/stats.rb +2 -2
- data/lib/miga/version.rb +4 -2
- data/scripts/essential_genes.bash +18 -3
- data/scripts/miga.bash +8 -2
- data/scripts/mytaxa.bash +6 -5
- data/scripts/mytaxa_scan.bash +8 -7
- data/scripts/ogs.bash +2 -3
- data/scripts/ssu.bash +16 -2
- data/test/dataset_test.rb +5 -5
- data/test/lair_test.rb +1 -2
- data/test/net_test.rb +34 -0
- data/test/with_option_test.rb +115 -0
- data/utils/FastAAI/00.Libraries/01.SCG_HMMs/Archaea_SCG.hmm +41964 -0
- data/utils/FastAAI/00.Libraries/01.SCG_HMMs/Bacteria_SCG.hmm +32439 -0
- data/utils/FastAAI/00.Libraries/01.SCG_HMMs/Complete_SCG_DB.hmm +62056 -0
- data/utils/FastAAI/FastAAI/FastAAI +1336 -0
- data/utils/FastAAI/README.md +84 -0
- data/utils/FastAAI/kAAI_v1.0_virus.py +1296 -0
- data/utils/cleanup-databases.rb +2 -3
- data/utils/distance/base.rb +9 -0
- data/utils/distance/commands.rb +183 -81
- data/utils/distance/database.rb +69 -10
- data/utils/distance/pipeline.rb +15 -21
- data/utils/distance/runner.rb +27 -49
- data/utils/distance/temporal.rb +4 -2
- data/utils/distances.rb +2 -2
- data/utils/index_metadata.rb +1 -2
- data/utils/requirements.txt +6 -5
- data/utils/subclade/runner.rb +10 -11
- metadata +18 -6
@@ -0,0 +1,119 @@
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# frozen_string_literal: true
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##
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4
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# Helper module with files configuration functions for MiGA::Cli::Action::Init
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5
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module MiGA::Cli::Action::Init::FilesHelper
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6
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+
def open_rc_file
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7
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rc_path = File.expand_path('.miga_rc', ENV['HOME'])
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8
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if File.exist? rc_path
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9
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if cli.ask_user(
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'I found a previous configuration. Do you want to continue?',
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'yes', %w(yes no)
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) == 'no'
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cli.puts 'OK, see you soon!'
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exit(0)
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end
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end
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rc_fh = File.open(rc_path, 'w')
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rc_fh.puts <<~BASH
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#!/bin/bash
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20
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# `miga init` made this on #{Time.now}
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BASH
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rc_fh
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end
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def close_rc_file(rc_fh)
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rc_fh.puts <<~FOOT
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MIGA_CONFIG_VERSION='#{MiGA::MiGA.FULL_VERSION}'
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MIGA_CONFIG_LONGVERSION='#{MiGA::MiGA.LONG_VERSION}'
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MIGA_CONFIG_DATE='#{Time.now}'
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FOOT
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rc_fh.close
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end
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def check_configuration_script(rc_fh)
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unless File.exist? cli[:config]
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cli[:config] = cli.ask_user(
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'Is there a script I need to load at startup?',
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cli[:config]
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)
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end
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if File.exist? cli[:config]
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cli[:config] = File.expand_path(cli[:config])
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cli.puts "Found bash configuration script: #{cli[:config]}"
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rc_fh.puts "MIGA_STARTUP='#{cli[:config]}'"
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rc_fh.puts '. "$MIGA_STARTUP"'
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else
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cli[:config] = '/dev/null'
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end
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cli.puts ''
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end
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def check_additional_files(paths)
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if cli[:mytaxa]
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check_mytaxa_scores(paths)
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check_mytaxa_database(paths)
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end
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check_rdp_classifier if cli[:rdp]
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check_phyla_lite
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cli.puts ''
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end
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def check_mytaxa_scores(paths)
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cli.print 'Looking for MyTaxa scores... '
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mt = File.dirname(paths['MyTaxa'])
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unless Dir.exist?(File.join(mt, 'db'))
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cli.puts "no\nExecute 'python2 #{mt}/utils/download_db.py'"
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raise 'Incomplete MyTaxa installation'
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end
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cli.puts 'yes'
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end
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def check_mytaxa_database(paths)
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cli.print 'Looking for MyTaxa DB... '
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mt = File.dirname(paths['MyTaxa'])
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dmnd_db = 'AllGenomes.faa.dmnd'
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miga_db = File.join(ENV['MIGA_HOME'], '.miga_db')
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home_db = File.join(miga_db, dmnd_db)
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mt_db = File.join(mt, 'AllGenomes.faa.dmnd')
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if File.exist?(home_db)
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cli.puts 'yes'
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elsif File.exist?(mt_db)
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cli.puts 'yes, sym-linking'
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File.symlink(mt_db, home_db)
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else
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cli.puts 'no, downloading'
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MiGA::MiGA.download_file_ftp(:miga_dist, dmnd_db, home_db) do |n, size|
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cli.advance("#{dmnd_db}:", n, size)
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end
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cli.puts
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end
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end
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def check_rdp_classifier
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cli.print 'Looking for RDP classifier... '
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miga_db = File.join(ENV['MIGA_HOME'], '.miga_db')
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file = 'classifier.jar'
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path = File.join(miga_db, file)
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if File.size?(path)
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cli.puts 'yes'
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else
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cli.puts 'no, downloading'
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arch = 'classifier.tar.gz'
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MiGA::MiGA.download_file_ftp(
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:miga_dist, arch, File.join(miga_db, arch)
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108
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) { |n, size| cli.advance("#{arch}:", n, size) }
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109
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`cd '#{miga_db}' && tar zxf '#{arch}' && rm '#{arch}'`
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110
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cli.puts
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111
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end
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end
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113
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def check_phyla_lite
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cli.puts 'Looking for Phyla Lite... '
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116
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cmd = ['get_db', '-n', 'Phyla_Lite', '--no-overwrite']
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117
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MiGA::Cli.new(cmd).launch(true)
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118
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end
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119
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end
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@@ -269,7 +269,7 @@ class MiGA::Cli::Action::NcbiGet < MiGA::Cli::Action
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269
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else
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270
270
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cli.say ' Creating dataset'
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rd.save_to(p, name, !cli[:query], body[:md])
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272
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cli.add_metadata(p.add_dataset(name))
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272
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cli.add_metadata(p.add_dataset(name))
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273
273
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end
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274
274
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end
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275
275
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end
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data/lib/miga/cli/action/new.rb
CHANGED
@@ -24,6 +24,11 @@ class MiGA::Cli::Action::New < MiGA::Cli::Action
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24
24
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'Use faster identity engines (Diamond-AAI and FastANI)',
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25
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'Equivalent to: -m aai_p=diamond,ani_p=fastani'
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26
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) { |v| cli[:fast] = v }
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27
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opt.on(
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28
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'--sensitive',
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29
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'Use more sensitive identity engines (BLAST+)',
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30
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'Equivalent to: -m aai_p=blast+,ani_p=blast+'
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31
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) { |v| cli[:sensitive] = v }
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27
32
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opt.on(
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28
33
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'-m', '--metadata STRING',
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29
34
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'Metadata as key-value pairs separated by = and delimited by comma',
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@@ -35,20 +40,21 @@ class MiGA::Cli::Action::New < MiGA::Cli::Action
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35
40
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def perform
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36
41
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cli.ensure_type(MiGA::Project)
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37
42
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cli.ensure_par(project: '-P')
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38
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-
unless
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39
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-
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40
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-
raise "You must initialize MiGA before creating the first project.\n" +
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41
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-
'Please use "miga init".'
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43
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unless MiGA::MiGA.initialized?
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44
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raise 'MiGA has not been initialized, please use "miga init" first'
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42
45
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end
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43
46
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cli.say "Creating project: #{cli[:project]}"
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44
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-
raise 'Project already exists, aborting
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47
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raise 'Project already exists, aborting' if Project.exist?(cli[:project])
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45
48
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46
49
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p = Project.new(cli[:project], false)
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47
50
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p = cli.add_metadata(p)
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48
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-
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49
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-
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50
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-
p.
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51
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+
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52
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if cli[:sensitive]
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53
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p.set_option(:aai_p, 'blast+')
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p.set_option(:ani_p, 'blast+')
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55
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elsif cli[:fast]
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56
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p.set_option(:aai_p, 'diamond')
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p.set_option(:ani_p, 'fastani')
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58
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end
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52
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p.save
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59
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end
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60
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end
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@@ -0,0 +1,44 @@
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# frozen_string_literal: true
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2
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3
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require 'miga/cli/action'
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4
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5
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##
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6
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# CLI: `miga option`
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7
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class MiGA::Cli::Action::Option < MiGA::Cli::Action
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8
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def parse_cli
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9
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cli.parse do |opt|
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10
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cli.opt_object(opt, %i[project dataset_opt])
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opt.on(
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'-k', '--key STRING',
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'Option name to get or set (by default, all options are printed)'
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14
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) { |v| cli[:key] = v }
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opt.on(
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16
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'--value STRING',
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'Value of the option to set (by default, option value is not changed)',
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18
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'Recognized tokens: nil, true, false'
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) { |v| cli[:value] = v }
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opt.on(
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'-o', '--output PATH',
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22
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'Create output file instead of returning to STDOUT'
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) { |v| cli[:output] = v }
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end
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25
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end
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def perform
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unless cli[:value].nil?
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cli.ensure_par(
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30
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{ key: '-k' },
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31
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'%<name>s is mandatory when --value is set: please provide %<flag>s'
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)
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end
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obj = cli.load_project_or_dataset
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35
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io = cli[:output].nil? ? $stdout : File.open(cli[:output], 'w')
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36
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if cli[:key].nil?
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37
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cli.table(%w[Key Value], obj.all_options.to_a, io)
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38
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else
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39
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obj.set_option(cli[:key], cli[:value], true) unless cli[:value].nil?
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40
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io.puts obj.option(cli[:key])
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41
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end
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io.close unless cli[:output].nil?
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end
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44
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end
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@@ -21,18 +21,20 @@ class MiGA::Cli::Action::PreprocWf < MiGA::Cli::Action
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'-m', '--mytaxa_scan',
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'Perform MyTaxa scan analysis'
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23
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) { |v| cli[:mytaxa] = v }
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24
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-
opts_for_wf(
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25
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-
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opts_for_wf(
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opt, 'Input files as defined by --input-type',
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26
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multi: true, cleanup: false, ncbi: false, project_type: true
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)
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end
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end
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def perform
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# Input data
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cli.ensure_par(input_type: '-i')
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-
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33
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-
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34
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-
.map { |i| ["run_#{i}", false] }
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34
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norun = %w[
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35
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project_stats haai_distances aai_distances ani_distances clade_finding
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36
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]
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p_metadata = Hash[norun.map { |i| ["run_#{i}", false] }]
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d_metadata = { run_distances: false }
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37
39
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unless cli[:mytaxa]
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d_metadata[:run_mytaxa_scan] = false
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@@ -21,10 +21,10 @@ class MiGA::Cli::Action::QualityWf < MiGA::Cli::Action
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21
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def perform
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# Input data
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24
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-
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25
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-
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26
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-
.map { |i| ["run_#{i}", false] }
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24
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+
norun = %w[
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25
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project_stats haai_distances aai_distances ani_distances clade_finding
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27
26
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]
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p_metadata = Hash[norun.map { |i| ["run_#{i}", false] }]
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28
28
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d_metadata = { run_distances: false }
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29
29
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d_metadata[:run_mytaxa_scan] = false unless cli[:mytaxa]
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30
30
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p = create_project(:assembly, p_metadata, d_metadata)
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@@ -47,7 +47,7 @@ class MiGA::Cli::Action::TaxDist < MiGA::Cli::Action
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|
47
47
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|
48
48
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def read_distances
|
49
49
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p = cli.load_project
|
50
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-
cli[:metric] ||= p.
|
50
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+
cli[:metric] ||= p.clade? ? 'ani' : 'aai'
|
51
51
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res_n = "#{cli[:metric]}_distances"
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52
52
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cli.say "Reading distances: 1-#{cli[:metric].upcase}"
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53
53
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res = p.result(res_n)
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@@ -59,7 +59,7 @@ class MiGA::Cli::Action::TaxTest < MiGA::Cli::Action
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59
59
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cli.puts "Closest relative: #{cr[0]} with AAI: #{cr[1]}."
|
60
60
|
p = cli.load_project
|
61
61
|
if cli[:ref_project]
|
62
|
-
if (ref = p.
|
62
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+
if (ref = p.option(:ref_project)).nil?
|
63
63
|
raise '--ref-project requested but no reference project has been set'
|
64
64
|
end
|
65
65
|
if (q = MiGA::Project.load(ref)).nil?
|
data/lib/miga/cli/action/wf.rb
CHANGED
@@ -7,9 +7,8 @@ module MiGA::Cli::Action::Wf
|
|
7
7
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def default_opts_for_wf
|
8
8
|
cli.expect_files = true
|
9
9
|
cli.defaults = {
|
10
|
-
clean: false,
|
11
|
-
|
12
|
-
ncbi_draft: true, min_qual: 25.0
|
10
|
+
clean: false, project_type: :genomes, dataset_type: :popgenome,
|
11
|
+
ncbi_draft: true, min_qual: MiGA::Project.OPTIONS[:min_qual][:default]
|
13
12
|
}
|
14
13
|
end
|
15
14
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|
@@ -56,28 +55,10 @@ module MiGA::Cli::Action::Wf
|
|
56
55
|
opt.on(
|
57
56
|
'-R', '--name-regexp REGEXP', Regexp,
|
58
57
|
'Regular expression indicating how to extract the name from the path',
|
59
|
-
"By default: '#{
|
58
|
+
"By default: '#{MiGA::Cli.FILE_REGEXP}'"
|
60
59
|
) { |v| cli[:regexp] = v }
|
61
|
-
opt
|
62
|
-
|
63
|
-
"Type of datasets. By default: #{cli[:dataset_type]}",
|
64
|
-
'Recognized types:',
|
65
|
-
*MiGA::Dataset.KNOWN_TYPES
|
66
|
-
.map do |k, v|
|
67
|
-
"~ #{k}: #{v[:description]}" unless !params[:multi] && v[:multi]
|
68
|
-
end.compact
|
69
|
-
) { |v| cli[:dataset_type] = v.downcase.to_sym }
|
70
|
-
if params[:project_type]
|
71
|
-
opt.on(
|
72
|
-
'--project-type STRING',
|
73
|
-
"Type of project. By default: #{cli[:project_type]}",
|
74
|
-
'Recognized types:',
|
75
|
-
*MiGA::Project.KNOWN_TYPES
|
76
|
-
.map do |k, v|
|
77
|
-
"~ #{k}: #{v[:description]}" unless !params[:multi] && v[:multi]
|
78
|
-
end.compact
|
79
|
-
) { |v| cli[:project_type] = v.downcase.to_sym }
|
80
|
-
end
|
60
|
+
opt_object_type(opt, :dataset, params[:multi])
|
61
|
+
opt_object_type(opt, :project, params[:multi]) if params[:project_type]
|
81
62
|
opt.on(
|
82
63
|
'--daemon PATH',
|
83
64
|
'Use custom daemon configuration in JSON format',
|
@@ -124,33 +105,42 @@ module MiGA::Cli::Action::Wf
|
|
124
105
|
project_type: '--project-type',
|
125
106
|
dataset_type: '--dataset-type'
|
126
107
|
)
|
108
|
+
paired = cli[:input_type].to_s.include?('_paired')
|
109
|
+
cli[:regexp] ||= MiGA::Cli.FILE_REGEXP(paired)
|
110
|
+
|
127
111
|
# Create empty project
|
128
|
-
call_cli(
|
129
|
-
|
130
|
-
|
131
|
-
|
132
|
-
]) unless MiGA::Project.exist? cli[:outdir]
|
112
|
+
call_cli(
|
113
|
+
['new', '-P', cli[:outdir], '-t', cli[:project_type]]
|
114
|
+
) unless MiGA::Project.exist? cli[:outdir]
|
115
|
+
|
133
116
|
# Define project metadata
|
134
117
|
p = cli.load_project(:outdir, '-o')
|
135
|
-
%i[haai_p aai_p ani_p ess_coll min_qual].each { |i| p_metadata[i] = cli[i] }
|
136
118
|
p_metadata[:type] = cli[:project_type]
|
137
119
|
transfer_metadata(p, p_metadata)
|
120
|
+
%i[haai_p aai_p ani_p ess_coll min_qual].each do |i|
|
121
|
+
p.set_option(i, cli[i])
|
122
|
+
end
|
123
|
+
|
138
124
|
# Download datasets
|
139
|
-
|
140
|
-
|
141
|
-
|
142
|
-
|
143
|
-
|
144
|
-
|
125
|
+
unless cli[:ncbi_taxon].nil?
|
126
|
+
what = cli[:ncbi_draft] ? '--all' : '--complete'
|
127
|
+
call_cli(
|
128
|
+
['ncbi_get', '-P', cli[:outdir], '-T', cli[:ncbi_taxon], what]
|
129
|
+
)
|
130
|
+
end
|
131
|
+
|
145
132
|
# Add datasets
|
146
|
-
call_cli(
|
147
|
-
|
148
|
-
|
149
|
-
|
150
|
-
|
151
|
-
|
152
|
-
|
153
|
-
|
133
|
+
call_cli(
|
134
|
+
[
|
135
|
+
'add',
|
136
|
+
'--ignore-dups',
|
137
|
+
'-P', cli[:outdir],
|
138
|
+
'-t', cli[:dataset_type],
|
139
|
+
'-i', stage,
|
140
|
+
'-R', cli[:regexp]
|
141
|
+
] + cli.files
|
142
|
+
) unless cli.files.empty?
|
143
|
+
|
154
144
|
# Define datasets metadata
|
155
145
|
p.load
|
156
146
|
d_metadata[:type] = cli[:dataset_type]
|
@@ -161,13 +151,13 @@ module MiGA::Cli::Action::Wf
|
|
161
151
|
def summarize(which = %w[cds assembly essential_genes ssu])
|
162
152
|
which.each do |r|
|
163
153
|
cli.say "Summary: #{r}"
|
164
|
-
call_cli(
|
165
|
-
|
166
|
-
|
167
|
-
|
168
|
-
|
169
|
-
|
170
|
-
|
154
|
+
call_cli(
|
155
|
+
[
|
156
|
+
'summary',
|
157
|
+
'-P', cli[:outdir], '-r', r, '--tab', '--ref', '--active',
|
158
|
+
'-o', File.join(cli[:outdir], "#{r}.tsv")
|
159
|
+
]
|
160
|
+
)
|
171
161
|
end
|
172
162
|
call_cli(['browse', '-P', cli[:outdir]])
|
173
163
|
end
|
@@ -193,17 +183,45 @@ module MiGA::Cli::Action::Wf
|
|
193
183
|
cmd += ['--max-jobs', cli[:jobs]] unless cli[:jobs].nil?
|
194
184
|
cmd += ['--ppn', cli[:threads]] unless cli[:threads].nil?
|
195
185
|
cwd = Dir.pwd
|
196
|
-
call_cli
|
186
|
+
call_cli(cmd)
|
197
187
|
Dir.chdir(cwd)
|
198
188
|
end
|
199
189
|
|
200
190
|
def transfer_metadata(obj, md)
|
201
191
|
# Clear old metadata
|
202
192
|
obj.metadata.each do |k, v|
|
203
|
-
obj.metadata[k] = nil if k.to_s =~ /^run_/ || k
|
193
|
+
obj.metadata[k] = nil if k.to_s =~ /^run_/ || obj.option?(k)
|
204
194
|
end
|
205
195
|
# Transfer and save
|
206
196
|
md.each { |k, v| obj.metadata[k] = v }
|
207
197
|
obj.save
|
208
198
|
end
|
199
|
+
|
200
|
+
private
|
201
|
+
|
202
|
+
##
|
203
|
+
# Add option --type or --project-type to +opt+
|
204
|
+
def opt_object_type(opt, obj, multi)
|
205
|
+
conf =
|
206
|
+
case obj
|
207
|
+
when :dataset
|
208
|
+
['type', 'datasets', :dataset_type, MiGA::Dataset]
|
209
|
+
when :project
|
210
|
+
['project-type', 'project', :project_type, MiGA::Project]
|
211
|
+
else
|
212
|
+
raise "Unrecognized object type: #{obj}"
|
213
|
+
end
|
214
|
+
|
215
|
+
options =
|
216
|
+
conf[3].KNOWN_TYPES.map do |k, v|
|
217
|
+
"~ #{k}: #{v[:description]}" unless !multi && v[:multi]
|
218
|
+
end.compact
|
219
|
+
|
220
|
+
opt.on(
|
221
|
+
"--#{conf[0]} STRING",
|
222
|
+
"Type of #{conf[1]}. By default: #{cli[conf[2]]}",
|
223
|
+
'Recognized types:',
|
224
|
+
*options
|
225
|
+
) { |v| cli[conf[2]] = v.downcase.to_sym }
|
226
|
+
end
|
209
227
|
end
|