miga-base 0.2.0.9 → 0.2.1.0
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- checksums.yaml +4 -4
- data/Rakefile +3 -0
- data/actions/add_result.rb +37 -0
- data/actions/add_taxonomy.rb +63 -0
- data/actions/create_dataset.rb +49 -0
- data/actions/create_project.rb +46 -0
- data/actions/daemon.rb +50 -0
- data/actions/date.rb +14 -0
- data/actions/{download_dataset → download_dataset.rb} +5 -28
- data/actions/find_datasets.rb +41 -0
- data/actions/import_datasets.rb +47 -0
- data/actions/index_taxonomy.rb +46 -0
- data/actions/list_datasets.rb +50 -0
- data/actions/list_files.rb +43 -0
- data/actions/project_info.rb +40 -0
- data/actions/unlink_dataset.rb +28 -0
- data/bin/miga +129 -33
- data/lib/miga/daemon.rb +48 -34
- data/lib/miga/dataset.rb +7 -123
- data/lib/miga/dataset_result.rb +177 -0
- data/lib/miga/project.rb +32 -12
- data/lib/miga/version.rb +2 -2
- data/scripts/_distances_functions.bash +82 -0
- data/scripts/_distances_noref_nomulti.bash +96 -67
- data/scripts/_distances_ref_nomulti.bash +54 -85
- data/scripts/assembly.bash +16 -3
- data/scripts/clade_finding.bash +20 -18
- data/scripts/distances.bash +2 -1
- data/scripts/init.bash +2 -6
- data/scripts/subclades.bash +4 -5
- data/test/common_test.rb +2 -2
- data/test/daemon_test.rb +73 -1
- data/test/project_test.rb +26 -2
- data/test/taxonomy_test.rb +10 -0
- data/test/test_helper.rb +1 -1
- data/utils/subclades-compile.rb +4 -2
- data/utils/subclades.R +140 -158
- metadata +48 -44
- data/actions/add_result +0 -58
- data/actions/add_taxonomy +0 -83
- data/actions/create_dataset +0 -61
- data/actions/create_project +0 -67
- data/actions/daemon +0 -66
- data/actions/find_datasets +0 -61
- data/actions/import_datasets +0 -83
- data/actions/index_taxonomy +0 -68
- data/actions/list_datasets +0 -81
- data/actions/list_files +0 -63
- data/actions/unlink_dataset +0 -49
data/lib/miga/dataset.rb
CHANGED
@@ -3,11 +3,14 @@
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3
3
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4
4
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require "miga/metadata"
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5
5
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require "miga/result"
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6
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+
require "miga/dataset_result"
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6
7
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7
8
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##
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8
9
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# Dataset representation in MiGA.
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9
10
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class MiGA::Dataset < MiGA::MiGA
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11
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12
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+
include MiGA::DatasetResult
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+
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11
14
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# Class-level
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15
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13
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##
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@@ -52,7 +55,7 @@ class MiGA::Dataset < MiGA::MiGA
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52
55
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53
56
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##
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54
57
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# Tasks to be excluded from query datasets.
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55
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-
@@EXCLUDE_NOREF_TASKS = [:
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58
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+
@@EXCLUDE_NOREF_TASKS = [:mytaxa_scan]
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56
59
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57
60
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##
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58
61
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# Tasks to be executed only in datasets that are not multi-organism. These
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@@ -137,14 +140,14 @@ class MiGA::Dataset < MiGA::MiGA
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137
140
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# Is this dataset known to be multi-organism?
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138
141
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def is_multi?
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139
142
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return false if self.metadata[:type].nil?
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140
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-
|
143
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+
@@KNOWN_TYPES[self.metadata[:type]][:multi]
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144
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end
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142
145
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143
146
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##
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144
147
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# Is this dataset known to be single-organism?
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145
148
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def is_nonmulti?
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146
149
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return false if self.metadata[:type].nil?
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147
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-
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150
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+
!@@KNOWN_TYPES[self.metadata[:type]][:multi]
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148
151
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end
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149
152
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150
153
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##
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@@ -176,7 +179,7 @@ class MiGA::Dataset < MiGA::MiGA
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return nil if @@RESULT_DIRS[result_type].nil?
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177
180
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base = project.path + "/data/" + @@RESULT_DIRS[result_type] +
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181
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"/" + name
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179
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-
return MiGA::Result.load(base
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182
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+
return MiGA::Result.load("#{base}.json") unless save
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180
183
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return nil unless result_files_exist?(base, ".done")
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181
184
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r = self.send("add_result_#{result_type}", base)
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185
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r.save unless r.nil?
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@@ -241,123 +244,4 @@ class MiGA::Dataset < MiGA::MiGA
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241
244
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adv
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242
245
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end
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243
246
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244
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-
private
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245
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-
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246
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-
def add_result_raw_reads(base)
|
247
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-
return nil unless result_files_exist?(base, ".1.fastq")
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248
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-
r = MiGA::Result.new(base + ".json")
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249
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-
add_files_to_ds_result(r, name,
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250
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-
( result_files_exist?(base, ".2.fastq") ?
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251
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-
{:pair1=>".1.fastq", :pair2=>".2.fastq"} :
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252
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-
{:single=>".1.fastq"} ))
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253
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-
end
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-
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255
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-
def add_result_trimmed_reads(base)
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-
return nil unless result_files_exist?(base, ".1.clipped.fastq")
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257
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-
r = MiGA::Result.new base + ".json"
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258
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-
r = add_files_to_ds_result(r, name,
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259
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-
{:pair1=>".1.clipped.fastq", :pair2=>".2.clipped.fastq"}) if
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260
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-
result_files_exist?(base, ".2.clipped.fastq")
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261
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-
r.add_file(:single, name + ".1.clipped.single.fastq")
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262
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-
add_result(:raw_reads) #-> Post gunzip
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263
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-
r
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264
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-
end
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265
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-
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266
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-
def add_result_read_quality(base)
|
267
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-
return nil unless result_files_exist?(base, %w[.solexaqa .fastqc])
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268
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-
r = MiGA::Result.new(base + ".json")
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269
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-
r = add_files_to_ds_result(r, name,
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270
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-
{:solexaqa=>".solexaqa", :fastqc=>".fastqc"})
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271
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-
add_result(:trimmed_reads) #-> Post cleaning
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272
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-
r
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273
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-
end
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274
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-
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275
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-
def add_result_trimmed_fasta(base)
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276
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-
return nil unless
|
277
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-
result_files_exist?(base, ".CoupledReads.fa") or
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278
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-
result_files_exist?(base, ".SingleReads.fa")
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279
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-
r = MiGA::Result.new base + ".json"
|
280
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-
r = add_files_to_ds_result(r, name, {:coupled=>".CoupledReads.fa",
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281
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-
:pair1=>".1.fa", :pair2=>".2.fa"}) if
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282
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-
result_files_exist?(base, ".CoupledReads.fa")
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283
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-
r.add_file(:single, name + ".SingleReads.fa")
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284
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add_result(:raw_reads) #-> Post gzip
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285
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-
r
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286
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-
end
|
287
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-
|
288
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-
def add_result_assembly(base)
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289
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-
return nil unless result_files_exist?(base, ".LargeContigs.fna")
|
290
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-
r = MiGA::Result.new(base + ".json")
|
291
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-
add_files_to_ds_result(r, name, {:largecontigs=>".LargeContigs.fna",
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292
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-
:allcontigs=>".AllContigs.fna"})
|
293
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-
end
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294
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-
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295
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-
def add_result_cds(base)
|
296
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-
return nil unless result_files_exist?(base, %w[.faa .fna])
|
297
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-
r = MiGA::Result.new(base + ".json")
|
298
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-
add_files_to_ds_result(r, name, {:proteins=>".faa", :genes=>".fna",
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299
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-
:gff2=>".gff2", :gff3=>".gff3", :tab=>".tab"})
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300
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-
end
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301
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-
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302
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-
def add_result_essential_genes(base)
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303
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-
return nil unless result_files_exist?(base, %w[.ess.faa .ess .ess/log])
|
304
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-
r = MiGA::Result.new(base + ".json")
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305
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-
add_files_to_ds_result(r, name, {:ess_genes=>".ess.faa",
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306
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-
:collection=>".ess", :report=>".ess/log"})
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307
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-
end
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308
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-
|
309
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-
def add_result_ssu(base)
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310
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-
return MiGA::Result.new(base + ".json") if result(:assembly).nil?
|
311
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-
return nil unless result_files_exist?(base, ".ssu.fa")
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312
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-
r = MiGA::Result.new(base + ".json")
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313
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-
add_files_to_ds_result(r, name, {:longest_ssu_gene=>".ssu.fa",
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314
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-
:gff=>".ssu.gff", :all_ssu_genes=>".ssu.all.fa"})
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315
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-
end
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316
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-
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317
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-
def add_result_mytaxa(base)
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318
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-
if is_multi?
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319
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-
return nil unless result_files_exist?(base, ".mytaxa")
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320
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-
r = MiGA::Result.new(base + ".json")
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321
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-
add_files_to_ds_result(r, name, {:mytaxa=>".mytaxa", :blast=>".blast",
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322
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-
:mytaxain=>".mytaxain"})
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323
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-
else
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324
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MiGA::Result.new base + ".json"
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325
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-
end
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326
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-
end
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327
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-
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328
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-
def add_result_mytaxa_scan(base)
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329
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-
if is_nonmulti?
|
330
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-
return nil unless
|
331
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-
result_files_exist?(base, %w[.pdf .wintax .mytaxa .reg])
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332
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-
r = MiGA::Result.new(base + ".json")
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333
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-
add_files_to_ds_result(r, name, {:mytaxa=>".mytaxa", :wintax=>".wintax",
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334
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-
:blast=>".blast", :mytaxain=>".mytaxain", :report=>".pdf",
|
335
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-
:regions=>".reg", :gene_ids=>".wintax.genes",
|
336
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-
:region_ids=>".wintax.regions"})
|
337
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-
else
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338
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-
MiGA::Result.new base + ".json"
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339
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-
end
|
340
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-
end
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341
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-
|
342
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-
def add_result_distances(base)
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343
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-
if is_nonmulti?
|
344
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-
pref = File.dirname(base)
|
345
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-
return nil unless
|
346
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-
File.exist?("#{pref}/#{is_ref? ? "01.haai" : "02.aai"}/#{name}.db")
|
347
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-
r = MiGA::Result.new(base + ".json")
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348
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-
r.add_files({:haai_db=>"01.haai/#{name}.db",
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349
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-
:aai_db=>"02.aai/#{name}.db", :ani_db=>"03.ani/#{name}.db"})
|
350
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-
else
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351
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r = MiGA::Result.new "#{base}.json"
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352
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-
end
|
353
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-
r
|
354
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-
end
|
355
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-
|
356
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-
def add_files_to_ds_result(r, name, rel_files)
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357
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-
files = {}
|
358
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-
rel_files.each{ |k,v| files[k] = name + v }
|
359
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-
r.add_files(files)
|
360
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-
r
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361
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-
end
|
362
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-
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363
247
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end # class MiGA::Dataset
|
@@ -0,0 +1,177 @@
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1
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+
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2
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+
##
|
3
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+
# Helper module including specific functions to add dataset results.
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4
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+
module MiGA::DatasetResult
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5
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+
|
6
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+
private
|
7
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+
|
8
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+
##
|
9
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+
# Add result type +:raw_reads+ at +base+.
|
10
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+
def add_result_raw_reads(base)
|
11
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+
return nil unless result_files_exist?(base, ".1.fastq")
|
12
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+
r = MiGA::Result.new(base + ".json")
|
13
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+
add_files_to_ds_result(r, name,
|
14
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+
( result_files_exist?(base, ".2.fastq") ?
|
15
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+
{:pair1=>".1.fastq", :pair2=>".2.fastq"} :
|
16
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+
{:single=>".1.fastq"} ))
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17
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+
end
|
18
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+
|
19
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+
##
|
20
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+
# Add result type +:trimmed_reads+ at +base+.
|
21
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+
def add_result_trimmed_reads(base)
|
22
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+
return nil unless result_files_exist?(base, ".1.clipped.fastq")
|
23
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+
r = MiGA::Result.new base + ".json"
|
24
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+
r = add_files_to_ds_result(r, name,
|
25
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+
{:pair1=>".1.clipped.fastq", :pair2=>".2.clipped.fastq"}) if
|
26
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+
result_files_exist?(base, ".2.clipped.fastq")
|
27
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+
r.add_file(:single, name + ".1.clipped.single.fastq")
|
28
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+
add_result(:raw_reads) #-> Post gunzip
|
29
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+
r
|
30
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+
end
|
31
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+
|
32
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+
##
|
33
|
+
# Add result type +:read_quality+ at +base+.
|
34
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+
def add_result_read_quality(base)
|
35
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+
return nil unless result_files_exist?(base, %w[.solexaqa .fastqc])
|
36
|
+
r = MiGA::Result.new(base + ".json")
|
37
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+
r = add_files_to_ds_result(r, name,
|
38
|
+
{:solexaqa=>".solexaqa", :fastqc=>".fastqc"})
|
39
|
+
add_result(:trimmed_reads) #-> Post cleaning
|
40
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+
r
|
41
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+
end
|
42
|
+
|
43
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+
##
|
44
|
+
# Add result type +:trimmed_fasta+ at +base+.
|
45
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+
def add_result_trimmed_fasta(base)
|
46
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+
return nil unless
|
47
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+
result_files_exist?(base, ".CoupledReads.fa") or
|
48
|
+
result_files_exist?(base, ".SingleReads.fa") or
|
49
|
+
result_files_exist?(base, %w[.1.fasta .2.fasta])
|
50
|
+
r = MiGA::Result.new base + ".json"
|
51
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+
r = add_files_to_ds_result(r, name, {:coupled=>".CoupledReads.fa",
|
52
|
+
:single=>".SingleReads.fa", :pair1=>".1.fasta", :pair2=>".2.fasta"})
|
53
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+
add_result(:raw_reads) #-> Post gzip
|
54
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+
r
|
55
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+
end
|
56
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+
|
57
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+
##
|
58
|
+
# Add result type +:assembly+ at +base+.
|
59
|
+
def add_result_assembly(base)
|
60
|
+
return nil unless result_files_exist?(base, ".LargeContigs.fna")
|
61
|
+
r = MiGA::Result.new(base + ".json")
|
62
|
+
add_files_to_ds_result(r, name, {:largecontigs=>".LargeContigs.fna",
|
63
|
+
:allcontigs=>".AllContigs.fna"})
|
64
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+
end
|
65
|
+
|
66
|
+
##
|
67
|
+
# Add result type +:cds+ at +base+.
|
68
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+
def add_result_cds(base)
|
69
|
+
return nil unless result_files_exist?(base, %w[.faa .fna])
|
70
|
+
r = MiGA::Result.new(base + ".json")
|
71
|
+
add_files_to_ds_result(r, name, {:proteins=>".faa", :genes=>".fna",
|
72
|
+
:gff2=>".gff2", :gff3=>".gff3", :tab=>".tab"})
|
73
|
+
end
|
74
|
+
|
75
|
+
##
|
76
|
+
# Add result type +:essential_genes+ at +base+.
|
77
|
+
def add_result_essential_genes(base)
|
78
|
+
return nil unless result_files_exist?(base, %w[.ess.faa .ess .ess/log])
|
79
|
+
r = MiGA::Result.new(base + ".json")
|
80
|
+
add_files_to_ds_result(r, name, {:ess_genes=>".ess.faa",
|
81
|
+
:collection=>".ess", :report=>".ess/log"})
|
82
|
+
end
|
83
|
+
|
84
|
+
##
|
85
|
+
# Add result type +:ssu+ at +base+.
|
86
|
+
def add_result_ssu(base)
|
87
|
+
return MiGA::Result.new(base + ".json") if result(:assembly).nil?
|
88
|
+
return nil unless result_files_exist?(base, ".ssu.fa")
|
89
|
+
r = MiGA::Result.new(base + ".json")
|
90
|
+
add_files_to_ds_result(r, name, {:longest_ssu_gene=>".ssu.fa",
|
91
|
+
:gff=>".ssu.gff", :all_ssu_genes=>".ssu.all.fa"})
|
92
|
+
end
|
93
|
+
|
94
|
+
##
|
95
|
+
# Add result type +:mytaxa+ at +base+.
|
96
|
+
def add_result_mytaxa(base)
|
97
|
+
if is_multi?
|
98
|
+
return nil unless result_files_exist?(base, ".mytaxa")
|
99
|
+
r = MiGA::Result.new(base + ".json")
|
100
|
+
add_files_to_ds_result(r, name, {:mytaxa=>".mytaxa", :blast=>".blast",
|
101
|
+
:mytaxain=>".mytaxain"})
|
102
|
+
else
|
103
|
+
MiGA::Result.new base + ".json"
|
104
|
+
end
|
105
|
+
end
|
106
|
+
|
107
|
+
##
|
108
|
+
# Add result type +:mytaxa_scan+ at +base+.
|
109
|
+
def add_result_mytaxa_scan(base)
|
110
|
+
if is_nonmulti?
|
111
|
+
return nil unless
|
112
|
+
result_files_exist?(base, %w[.pdf .wintax .mytaxa .reg])
|
113
|
+
r = MiGA::Result.new(base + ".json")
|
114
|
+
add_files_to_ds_result(r, name, {:mytaxa=>".mytaxa", :wintax=>".wintax",
|
115
|
+
:blast=>".blast", :mytaxain=>".mytaxain", :report=>".pdf",
|
116
|
+
:regions=>".reg", :gene_ids=>".wintax.genes",
|
117
|
+
:region_ids=>".wintax.regions"})
|
118
|
+
else
|
119
|
+
MiGA::Result.new base + ".json"
|
120
|
+
end
|
121
|
+
end
|
122
|
+
|
123
|
+
##
|
124
|
+
# Add result type +:distances+ at +base+.
|
125
|
+
def add_result_distances(base)
|
126
|
+
if is_nonmulti?
|
127
|
+
if is_ref?
|
128
|
+
add_result_distances_ref(base)
|
129
|
+
else
|
130
|
+
add_result_distances_nonref(base)
|
131
|
+
end
|
132
|
+
else
|
133
|
+
add_result_distances_multi(base)
|
134
|
+
end
|
135
|
+
end
|
136
|
+
|
137
|
+
##
|
138
|
+
# Add result type +:distances+ for _multi_ datasets at +base+.
|
139
|
+
def add_result_distances_multi(base)
|
140
|
+
MiGA::Result.new "#{base}.json"
|
141
|
+
end
|
142
|
+
|
143
|
+
##
|
144
|
+
# Add result type +:distances+ for _nonmulti_ reference datasets at +base+.
|
145
|
+
def add_result_distances_ref(base)
|
146
|
+
pref = File.dirname(base)
|
147
|
+
return nil unless
|
148
|
+
File.exist?("#{pref}/01.haai/#{name}.db")
|
149
|
+
r = MiGA::Result.new(base + ".json")
|
150
|
+
r.add_files({:haai_db=>"01.haai/#{name}.db",
|
151
|
+
:aai_db=>"02.aai/#{name}.db", :ani_db=>"03.ani/#{name}.db"})
|
152
|
+
r
|
153
|
+
end
|
154
|
+
|
155
|
+
##
|
156
|
+
# Add result type +:distances+ for _nonmulti_ query datasets at +base+.
|
157
|
+
def add_result_distances_nonref(base)
|
158
|
+
return nil unless
|
159
|
+
result_files_exist?(base, %w[.aai-medoids.tsv .aai.db]) or
|
160
|
+
result_files_exist?(base, %w[.ani-medoids.tsv .ani.db])
|
161
|
+
r = MiGA::Result.new(base + ".json")
|
162
|
+
add_files_to_ds_result(r, name, {
|
163
|
+
:aai_medoids=>".aai-medoids.tsv",
|
164
|
+
:haai_db=>".haai.db", :aai_db=>".aai.db",
|
165
|
+
:ani_medoids=>".ani-medoids.tsv", :ani_db=>".ani.db"})
|
166
|
+
end
|
167
|
+
|
168
|
+
##
|
169
|
+
# Add files in +rel_files+ Hash to the result +r+ with dataset name +name+.
|
170
|
+
def add_files_to_ds_result(r, name, rel_files)
|
171
|
+
files = {}
|
172
|
+
rel_files.each{ |k,v| files[k] = name + v }
|
173
|
+
r.add_files(files)
|
174
|
+
r
|
175
|
+
end
|
176
|
+
|
177
|
+
end
|
data/lib/miga/project.rb
CHANGED
@@ -146,12 +146,26 @@ class MiGA::Project < MiGA::MiGA
|
|
146
146
|
##
|
147
147
|
# Name of the project.
|
148
148
|
def name ; metadata[:name] ; end
|
149
|
+
|
150
|
+
##
|
151
|
+
# Type of project.
|
152
|
+
def type ; metadata[:type] ; end
|
153
|
+
|
154
|
+
##
|
155
|
+
# Is this a clade project?
|
156
|
+
def is_clade? ; type==:clade ; end
|
149
157
|
|
150
158
|
##
|
151
159
|
# Returns Array of MiGA::Dataset.
|
152
160
|
def datasets
|
153
161
|
metadata[:datasets].map{ |name| dataset(name) }
|
154
162
|
end
|
163
|
+
|
164
|
+
##
|
165
|
+
# Returns Array of String (without evaluating dataset objects).
|
166
|
+
def dataset_names
|
167
|
+
metadata[:datasets]
|
168
|
+
end
|
155
169
|
|
156
170
|
##
|
157
171
|
# Returns MiGA::Dataset.
|
@@ -164,17 +178,24 @@ class MiGA::Project < MiGA::MiGA
|
|
164
178
|
end
|
165
179
|
|
166
180
|
##
|
167
|
-
# Iterate through datasets, with
|
168
|
-
#
|
181
|
+
# Iterate through datasets, with one or two variables passed to +blk+.
|
182
|
+
# If one, the dataset MiGA::Dataset object is passed. If two, the name and
|
183
|
+
# the dataset object are passed.
|
169
184
|
def each_dataset(&blk)
|
170
|
-
metadata[:datasets].each
|
185
|
+
metadata[:datasets].each do |name|
|
186
|
+
if blk.arity == 1
|
187
|
+
blk.call(dataset(name))
|
188
|
+
else
|
189
|
+
blk.call(name, dataset(name))
|
190
|
+
end
|
191
|
+
end
|
171
192
|
end
|
172
193
|
|
173
194
|
##
|
174
195
|
# Add dataset identified by +name+ and return MiGA::Dataset.
|
175
196
|
def add_dataset(name)
|
176
197
|
unless metadata[:datasets].include? name
|
177
|
-
|
198
|
+
MiGA::Dataset.new(self, name)
|
178
199
|
@metadata[:datasets] << name
|
179
200
|
save
|
180
201
|
end
|
@@ -324,7 +345,9 @@ class MiGA::Project < MiGA::MiGA
|
|
324
345
|
|
325
346
|
def add_result_clade_finding(base)
|
326
347
|
return nil unless result_files_exist?(base,
|
327
|
-
%w[.proposed-clades
|
348
|
+
%w[.proposed-clades])
|
349
|
+
return nil unless is_clade? or result_files_exist?(base,
|
350
|
+
%w[.pdf .classif .medoids .class.tsv .class.nwk])
|
328
351
|
r = add_result_iter_clades(base)
|
329
352
|
r.add_file(:aai_tree, "miga-project.aai.nwk")
|
330
353
|
r.add_file(:proposal, "miga-project.proposed-clades")
|
@@ -335,9 +358,9 @@ class MiGA::Project < MiGA::MiGA
|
|
335
358
|
|
336
359
|
def add_result_subclades(base)
|
337
360
|
return nil unless result_files_exist?(base,
|
338
|
-
%w[.pdf .
|
361
|
+
%w[.pdf .classif .medoids .class.tsv .class.nwk])
|
339
362
|
r = add_result_iter_clades(base)
|
340
|
-
r.add_file(:ani_tree,
|
363
|
+
r.add_file(:ani_tree, "miga-project.ani.nwk")
|
341
364
|
r
|
342
365
|
end
|
343
366
|
|
@@ -346,11 +369,8 @@ class MiGA::Project < MiGA::MiGA
|
|
346
369
|
r.add_file(:report, "miga-project.pdf")
|
347
370
|
r.add_file(:class_table, "miga-project.class.tsv")
|
348
371
|
r.add_file(:class_tree, "miga-project.class.nwk")
|
349
|
-
(
|
350
|
-
|
351
|
-
r.add_file("#{m}_#{i}".to_sym, "miga-project.#{i}.#{m}")
|
352
|
-
end
|
353
|
-
end
|
372
|
+
r.add_file(:classif, "miga-project.classif")
|
373
|
+
r.add_file(:medoids, "miga-project.medoids")
|
354
374
|
r
|
355
375
|
end
|
356
376
|
|
data/lib/miga/version.rb
CHANGED
@@ -10,7 +10,7 @@ module MiGA
|
|
10
10
|
# - Float representing the major.minor version.
|
11
11
|
# - Integer representing gem releases of the current version.
|
12
12
|
# - Integer representing minor changes that require new version number.
|
13
|
-
VERSION = [0.2,
|
13
|
+
VERSION = [0.2, 1, 0]
|
14
14
|
|
15
15
|
##
|
16
16
|
# Nickname for the current major.minor version.
|
@@ -18,7 +18,7 @@ module MiGA
|
|
18
18
|
|
19
19
|
##
|
20
20
|
# Date of the current gem release.
|
21
|
-
VERSION_DATE = Date.new(2016, 04,
|
21
|
+
VERSION_DATE = Date.new(2016, 04, 20)
|
22
22
|
|
23
23
|
##
|
24
24
|
# Reference of MiGA.
|