miga-base 0.2.0.6 → 0.2.0.7
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- checksums.yaml +4 -4
- data/Gemfile +3 -0
- data/LICENSE +201 -0
- data/README.md +17 -335
- data/Rakefile +31 -0
- data/actions/add_result +2 -5
- data/actions/add_taxonomy +4 -7
- data/actions/create_dataset +5 -6
- data/actions/create_project +2 -5
- data/actions/daemon +2 -5
- data/actions/download_dataset +88 -58
- data/actions/find_datasets +36 -38
- data/actions/import_datasets +2 -5
- data/actions/index_taxonomy +2 -5
- data/actions/list_datasets +47 -49
- data/actions/list_files +7 -11
- data/actions/unlink_dataset +2 -5
- data/bin/miga +1 -1
- data/lib/miga/common.rb +132 -0
- data/lib/miga/daemon.rb +229 -168
- data/lib/miga/dataset.rb +354 -277
- data/lib/miga/gui.rb +346 -269
- data/lib/miga/metadata.rb +115 -71
- data/lib/miga/project.rb +361 -259
- data/lib/miga/remote_dataset.rb +200 -148
- data/lib/miga/result.rb +150 -99
- data/lib/miga/tax_index.rb +124 -67
- data/lib/miga/taxonomy.rb +129 -100
- data/lib/miga/version.rb +57 -0
- data/lib/miga.rb +2 -77
- data/scripts/_distances_noref_nomulti.bash +2 -0
- data/scripts/_distances_ref_nomulti.bash +2 -0
- data/scripts/aai_distances.bash +1 -0
- data/scripts/ani_distances.bash +1 -0
- data/scripts/assembly.bash +1 -0
- data/scripts/cds.bash +1 -0
- data/scripts/clade_finding.bash +17 -1
- data/scripts/distances.bash +1 -0
- data/scripts/essential_genes.bash +1 -0
- data/scripts/haai_distances.bash +1 -0
- data/scripts/init.bash +2 -0
- data/scripts/mytaxa.bash +1 -0
- data/scripts/mytaxa_scan.bash +1 -0
- data/scripts/ogs.bash +1 -0
- data/scripts/read_quality.bash +1 -0
- data/scripts/ssu.bash +1 -0
- data/scripts/subclades.bash +1 -0
- data/scripts/trimmed_fasta.bash +1 -0
- data/scripts/trimmed_reads.bash +1 -0
- data/test/common_test.rb +82 -0
- data/test/daemon_test.rb +53 -0
- data/test/dataset_test.rb +156 -0
- data/test/jruby_gui_test.rb +20 -0
- data/test/metadata_test.rb +48 -0
- data/test/project_test.rb +54 -0
- data/test/remote_dataset_test.rb +41 -0
- data/test/tax_index_test.rb +44 -0
- data/test/taxonomy_test.rb +36 -0
- data/test/test_helper.rb +32 -0
- metadata +53 -38
data/lib/miga/dataset.rb
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# @package MiGA
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# @
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# @license artistic license 2.0
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# @update Jan-18-2016
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# @license Artistic-2.0
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require "miga/metadata"
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require "miga/project"
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require "miga/result"
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##
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# Dataset representation in MiGA.
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class MiGA::Dataset < MiGA::MiGA
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# Class-level
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##
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# Directories containing the results from dataset-specific tasks.
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def self.RESULT_DIRS ; @@RESULT_DIRS end
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@@RESULT_DIRS = {
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# Preprocessing
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raw_reads: "01.raw_reads", trimmed_reads: "02.trimmed_reads",
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read_quality: "03.read_quality", trimmed_fasta: "04.trimmed_fasta",
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assembly: "05.assembly", cds: "06.cds",
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# Annotation
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essential_genes: "07.annotation/01.function/01.essential",
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ssu: "07.annotation/01.function/02.ssu",
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mytaxa: "07.annotation/02.taxonomy/01.mytaxa",
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mytaxa_scan: "07.annotation/03.qa/02.mytaxa_scan",
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# Mapping
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mapping_on_contigs: "08.mapping/01.read-ctg",
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mapping_on_genes: "08.mapping/02.read-gene",
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# Distances (for single-species datasets)
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distances: "09.distances"
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}
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##
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# Supported dataset types.
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def self.KNOWN_TYPES ; @@KNOWN_TYPES end
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@@KNOWN_TYPES = {
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genome: {description: "The genome from an isolate.", multi: false},
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metagenome: {description: "A metagenome (excluding viromes).",
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multi: true},
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virome: {description: "A viral metagenome.", multi: true},
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scgenome: {description: "A genome from a single cell.", multi: false},
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popgenome: {description: "The genome of a population (including " +
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"microdiversity).", :multi=>false}
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}
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##
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# Returns an Array of tasks to be executed before project-wide tasks.
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def self.PREPROCESSING_TASKS ; @@PREPROCESSING_TASKS ; end
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@@PREPROCESSING_TASKS = [:raw_reads, :trimmed_reads, :read_quality,
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:trimmed_fasta, :assembly, :cds, :essential_genes, :ssu, :mytaxa,
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:mytaxa_scan, :distances]
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##
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# Tasks to be excluded from query datasets.
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@@EXCLUDE_NOREF_TASKS = [:essential_genes, :mytaxa_scan]
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##
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# Tasks to be executed only in datasets that are not multi-organism. These
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# tasks are ignored for multi-organism datasets or for unknown types.
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@@ONLY_NONMULTI_TASKS = [:mytaxa_scan, :distances]
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##
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# Tasks to be executed only in datasets that are multi-organism. These
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# tasks are ignored for single-organism datasets or for unknwon types.
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@@ONLY_MULTI_TASKS = [:mytaxa]
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##
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# Does the +project+ already have a dataset with that +name+?
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def self.exist?(project, name)
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File.exist? project.path + "/metadata/" + name + ".json"
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end
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##
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# Standard fields of metadata for datasets.
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def self.INFO_FIELDS
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%w(name created updated type ref user description comments)
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end
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# Instance-level
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##
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# MiGA::Project that contains the dataset.
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attr_reader :project
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##
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# Datasets are uniquely identified by +name+ in a project.
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attr_reader :name
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##
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# MiGA::Metadata with information about the dataset.
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attr_reader :metadata
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##
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# Create a MiGA::Dataset object in a +project+ MiGA::Project with a
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# uniquely identifying +name+. +is_ref+ indicates if the dataset is to
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# be treated as reference (true, default) or query (false). Pass any
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# additional +metadata+ as a Hash.
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def initialize(project, name, is_ref=true, metadata={})
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raise "Invalid name '#{name}', please use only alphanumerics and " +
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"underscores." unless name.miga_name?
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@project = project
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@name = name
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metadata[:ref] = is_ref
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@metadata = MiGA::Metadata.new(project.path + "/metadata/" + name + ".json",
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metadata)
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end
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##
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# Save any changes you've made in the dataset.
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def save
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self.metadata[:type] = :metagenome if !metadata[:tax].nil? and
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!metadata[:tax][:ns].nil? and metadata[:tax][:ns]=="COMMUNITY"
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self.metadata.save
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end
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##
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# Delete the dataset with all it's contents (including results) and returns
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# nil.
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def remove!
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self.results.each{ |r| r.remove! }
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self.metadata.remove!
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end
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##
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# Get standard metadata values for the dataset as Array.
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def info
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MiGA::Dataset.INFO_FIELDS.map do |k|
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(k=="name") ? self.name : self.metadata[k.to_sym]
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end
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end
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##
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# Is this dataset a reference?
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def is_ref? ; !!self.metadata[:ref] ; end
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##
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# Is this dataset known to be multi-organism?
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def is_multi?
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return false if self.metadata[:type].nil?
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return @@KNOWN_TYPES[self.metadata[:type]][:multi]
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end
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##
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# Is this dataset known to be single-organism?
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def is_nonmulti?
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return false if self.metadata[:type].nil?
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return !@@KNOWN_TYPES[self.metadata[:type]][:multi]
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end
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##
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# Get the result MiGA::Result in this dataset identified by the symbol +k+.
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def result(k)
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return nil if @@RESULT_DIRS[k.to_sym].nil?
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MiGA::Result.load(project.path + "/data/" + @@RESULT_DIRS[k.to_sym] +
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"/" + name + ".json")
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end
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##
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# Get all the results (Array of MiGA::Result) in this dataset.
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def results ; @@RESULT_DIRS.keys.map{ |k| result k }.compact ; end
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##
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# For each result executes the 2-ary +blk+ block: key symbol and MiGA::Result.
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def each_result(&blk)
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@@RESULT_DIRS.keys.each do |k|
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blk.call(k, result(k)) unless result(k).nil?
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end
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end
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##
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# Look for the result with symbol key +result_type+ and register it in the
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# dataset. If +save+ is false, it doesn't register the result, but it still
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# returns a result if the expected files are complete. Returns MiGA::Result
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# or nil.
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def add_result(result_type, save=true)
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return nil if @@RESULT_DIRS[result_type].nil?
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base = project.path + "/data/" + @@RESULT_DIRS[result_type] +
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"/" + name
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return MiGA::Result.load(base + ".json") unless save
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return nil unless result_files_exist?(base, ".done")
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+
r = self.send("add_result_#{result_type}", base)
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r.save unless r.nil?
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+
r
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end
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+
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##
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# Returns the key symbol of the first registered result (sorted by the
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# execution order). This typically corresponds to the result used as the
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# initial input. Passes +save+ to #add_result.
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def first_preprocessing(save=false)
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@@PREPROCESSING_TASKS.find{ |t| not add_result(t, save).nil? }
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+
end
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+
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##
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# Returns the key symbol of the next task that needs to be executed. Passes
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# +save+ to #add_result.
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def next_preprocessing(save=false)
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after_first = false
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first = first_preprocessing(save)
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return nil if first.nil?
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+
@@PREPROCESSING_TASKS.each do |t|
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next if ignore_task? t
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return t if after_first and add_result(t, save).nil?
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+
after_first = (after_first or (t==first))
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+
end
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+
nil
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+
end
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+
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+
##
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# Should I ignore +task+ for this dataset?
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+
def ignore_task?(task)
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( (@@EXCLUDE_NOREF_TASKS.include?(task) and not is_ref?) or
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(@@ONLY_MULTI_TASKS.include?(task) and not is_multi?) or
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(@@ONLY_NONMULTI_TASKS.include?(task) and not is_nonmulti?))
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+
end
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+
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+
##
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+
# Are all the dataset-specific tasks done? Passes +save+ to #add_result.
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+
def done_preprocessing?(save=false)
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+
!first_preprocessing(save).nil? and next_preprocessing(save).nil?
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+
end
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+
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+
##
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# Returns an array indicating the stage of each task (sorted by execution
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+
# order). The values are integers:
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+
# - 0 for an undefined result (a task before the initial input).
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+
# - 1 for a registered result (a completed task).
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+
# - 2 for a queued result (a task yet to be executed).
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+
# It passes +save+ to #add_result
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+
def profile_advance(save=false)
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+
first_task = first_preprocessing(save)
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|
+
return Array.new(@@PREPROCESSING_TASKS.size, 0) if first_task.nil?
|
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|
+
adv = []
|
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|
+
state = 0
|
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|
+
next_task = next_preprocessing(save)
|
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|
+
@@PREPROCESSING_TASKS.each do |task|
|
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|
+
state = 1 if first_task==task
|
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|
+
state = 2 if !next_task.nil? and next_task==task
|
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|
+
adv << state
|
240
|
+
end
|
241
|
+
adv
|
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|
+
end
|
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|
+
|
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|
+
private
|
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|
+
|
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|
+
def add_result_raw_reads(base)
|
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|
+
return nil unless result_files_exist?(base, ".1.fastq")
|
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|
+
r = MiGA::Result.new(base + ".json")
|
249
|
+
add_files_to_ds_result(r, name,
|
250
|
+
( result_files_exist?(base, ".2.fastq") ?
|
251
|
+
{:pair1=>".1.fastq", :pair2=>".2.fastq"} :
|
252
|
+
{:single=>".1.fastq"} ))
|
253
|
+
end
|
254
|
+
|
255
|
+
def add_result_trimmed_reads(base)
|
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|
+
return nil unless result_files_exist?(base, ".1.clipped.fastq")
|
257
|
+
r = MiGA::Result.new base + ".json"
|
258
|
+
r = add_files_to_ds_result(r, name,
|
259
|
+
{:pair1=>".1.clipped.fastq", :pair2=>".2.clipped.fastq"}) if
|
260
|
+
result_files_exist?(base, ".2.clipped.fastq")
|
261
|
+
r.add_file(:single, name + ".1.clipped.single.fastq")
|
262
|
+
add_result(:raw_reads) #-> Post gunzip
|
263
|
+
r
|
264
|
+
end
|
265
|
+
|
266
|
+
def add_result_read_quality(base)
|
267
|
+
return nil unless result_files_exist?(base, %w[.solexaqa .fastqc])
|
268
|
+
r = MiGA::Result.new(base + ".json")
|
269
|
+
r = add_files_to_ds_result(r, name,
|
270
|
+
{:solexaqa=>".solexaqa", :fastqc=>".fastqc"})
|
271
|
+
add_result(:trimmed_reads) #-> Post cleaning
|
272
|
+
r
|
273
|
+
end
|
274
|
+
|
275
|
+
def add_result_trimmed_fasta(base)
|
276
|
+
return nil unless
|
277
|
+
result_files_exist?(base, ".CoupledReads.fa") or
|
278
|
+
result_files_exist?(base, ".SingleReads.fa")
|
279
|
+
r = MiGA::Result.new base + ".json"
|
280
|
+
r = add_files_to_ds_result(r, name, {:coupled=>".CoupledReads.fa",
|
281
|
+
:pair1=>".1.fa", :pair2=>".2.fa"}) if
|
282
|
+
result_files_exist?(base, ".CoupledReads.fa")
|
283
|
+
r.add_file(:single, name + ".SingleReads.fa")
|
284
|
+
add_result(:raw_reads) #-> Post gzip
|
285
|
+
r
|
286
|
+
end
|
287
|
+
|
288
|
+
def add_result_assembly(base)
|
289
|
+
return nil unless result_files_exist?(base, ".LargeContigs.fna")
|
290
|
+
r = MiGA::Result.new(base + ".json")
|
291
|
+
add_files_to_ds_result(r, name, {:largecontigs=>".LargeContigs.fna",
|
292
|
+
:allcontigs=>".AllContigs.fna"})
|
293
|
+
end
|
294
|
+
|
295
|
+
def add_result_cds(base)
|
296
|
+
return nil unless result_files_exist?(base, %w[.faa .fna])
|
297
|
+
r = MiGA::Result.new(base + ".json")
|
298
|
+
add_files_to_ds_result(r, name, {:proteins=>".faa", :genes=>".fna",
|
299
|
+
:gff2=>".gff2", :gff3=>".gff3", :tab=>".tab"})
|
300
|
+
end
|
301
|
+
|
302
|
+
def add_result_essential_genes(base)
|
303
|
+
return nil unless result_files_exist?(base, %w[.ess.faa .ess .ess/log])
|
304
|
+
r = MiGA::Result.new(base + ".json")
|
305
|
+
add_files_to_ds_result(r, name, {:ess_genes=>".ess.faa",
|
306
|
+
:collection=>".ess", :report=>".ess/log"})
|
307
|
+
end
|
308
|
+
|
309
|
+
def add_result_ssu(base)
|
310
|
+
return MiGA::Result.new(base + ".json") if result(:assembly).nil?
|
311
|
+
return nil unless result_files_exist?(base, ".ssu.fa")
|
312
|
+
r = MiGA::Result.new(base + ".json")
|
313
|
+
add_files_to_ds_result(r, name, {:longest_ssu_gene=>".ssu.fa",
|
314
|
+
:gff=>".ssu.gff", :all_ssu_genes=>".ssu.all.fa"})
|
315
|
+
end
|
316
|
+
|
317
|
+
def add_result_mytaxa(base)
|
318
|
+
if is_multi?
|
319
|
+
return nil unless result_files_exist?(base, ".mytaxa")
|
320
|
+
r = MiGA::Result.new(base + ".json")
|
321
|
+
add_files_to_ds_result(r, name, {:mytaxa=>".mytaxa", :blast=>".blast",
|
322
|
+
:mytaxain=>".mytaxain"})
|
323
|
+
else
|
324
|
+
MiGA::Result.new base + ".json"
|
264
325
|
end
|
265
|
-
|
266
|
-
|
326
|
+
end
|
327
|
+
|
328
|
+
def add_result_mytaxa_scan(base)
|
329
|
+
if is_nonmulti?
|
330
|
+
return nil unless
|
331
|
+
result_files_exist?(base, %w[.pdf .wintax .mytaxa .reg])
|
332
|
+
r = MiGA::Result.new(base + ".json")
|
333
|
+
add_files_to_ds_result(r, name, {:mytaxa=>".mytaxa", :wintax=>".wintax",
|
334
|
+
:blast=>".blast", :mytaxain=>".mytaxain", :report=>".pdf",
|
335
|
+
:regions=>".reg", :gene_ids=>".wintax.genes",
|
336
|
+
:region_ids=>".wintax.regions"})
|
337
|
+
else
|
338
|
+
MiGA::Result.new base + ".json"
|
267
339
|
end
|
268
|
-
|
269
|
-
|
270
|
-
|
271
|
-
|
272
|
-
|
273
|
-
|
274
|
-
|
275
|
-
|
276
|
-
|
277
|
-
|
278
|
-
|
279
|
-
|
280
|
-
end
|
281
|
-
end
|
282
|
-
adv
|
340
|
+
end
|
341
|
+
|
342
|
+
def add_result_distances(base)
|
343
|
+
if is_nonmulti?
|
344
|
+
pref = File.dirname(base)
|
345
|
+
return nil unless
|
346
|
+
File.exist?("#{pref}/#{is_ref? ? "01.haai" : "02.aai"}/#{name}.db")
|
347
|
+
r = MiGA::Result.new(base + ".json")
|
348
|
+
r.add_files({:haai_db=>"01.haai/#{name}.db",
|
349
|
+
:aai_db=>"02.aai/#{name}.db", :ani_db=>"03.ani/#{name}.db"})
|
350
|
+
else
|
351
|
+
r = MiGA::Result.new "#{base}.json"
|
283
352
|
end
|
284
|
-
|
285
|
-
end
|
353
|
+
r
|
354
|
+
end
|
355
|
+
|
356
|
+
def add_files_to_ds_result(r, name, rel_files)
|
357
|
+
files = {}
|
358
|
+
rel_files.each{ |k,v| files[k] = name + v }
|
359
|
+
r.add_files(files)
|
360
|
+
r
|
361
|
+
end
|
286
362
|
|
363
|
+
end # class MiGA::Dataset
|