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+<fig id="fig-1">
+  <object-id pub-id-type="doi">10.7717/peerj.139/fig-1</object-id>
+  <label>Figure 1</label>
+  <caption>
+    <title>Seasonal differences</title>
+    <p>In testes collected from photostimulated male starlings.</p>
+  </caption>
+  <graphic mimetype="image" mime-subtype="png" xlink:href="https://peerj.com/articles/139/fig-1.png"/>
+</fig>

data/spec/fixtures/heading.xml

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+<sec sec-type="intro">
+	<title>Introduction</title>
+	<sec id="this-one">
+		<title>Materials and methods</title>
+	</sec>
+</sec>

data/spec/fixtures/peerj.xml

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+<?xml version="1.0" encoding="UTF-8"?>
+<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.0 20120330//EN" "http://jats.nlm.nih.gov/publishing/1.0/JATS-journalpublishing1.dtd">
+<article xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" article-type="research-article" dtd-version="1.0">
+  <front>
+    <journal-meta>
+      <journal-id journal-id-type="pmc">PeerJ</journal-id>
+      <journal-id journal-id-type="nlm-ta">PeerJ</journal-id>
+      <journal-id journal-id-type="publisher-id">PeerJ</journal-id>
+      <journal-title-group>
+        <journal-title>PeerJ</journal-title>
+      </journal-title-group>
+      <issn pub-type="epub">2167-8359</issn>
+      <publisher>
+        <publisher-name>PeerJ Inc.</publisher-name>
+        <publisher-loc>San Francisco, USA</publisher-loc>
+      </publisher>
+    </journal-meta>
+    <article-meta>
+      <article-id pub-id-type="publisher-id">139</article-id>
+      <article-id pub-id-type="doi">10.7717/peerj.139</article-id>
+      <article-categories>
+        <subj-group subj-group-type="categories">
+          <subject>Ecology</subject>
+          <subject>Zoology</subject>
+          <subject>Diabetes and Endocrinology</subject>
+        </subj-group>
+      </article-categories>
+      <title-group>
+        <article-title>The direct response of the gonads to cues of stress in a temperate songbird species is season-dependent</article-title>
+      </title-group>
+      <contrib-group content-type="authors">
+        <contrib id="author-1" contrib-type="author">
+          <name>
+            <surname>McGuire</surname>
+            <given-names>Nicolette L.</given-names>
+          </name>
+          <xref ref-type="aff" rid="aff-1">1</xref>
+        </contrib>
+        <contrib id="author-2" contrib-type="author">
+          <name>
+            <surname>Koh</surname>
+            <given-names>Annie</given-names>
+          </name>
+          <xref ref-type="aff" rid="aff-1">1</xref>
+        </contrib>
+        <contrib id="author-3" contrib-type="author" corresp="yes">
+          <name>
+            <surname>Bentley</surname>
+            <given-names>George E.</given-names>
+          </name>
+          <xref ref-type="aff" rid="aff-1">1</xref>
+          <xref ref-type="aff" rid="aff-2">2</xref>
+          <email>Gb7@berkeley.edu</email>
+        </contrib>
+        <aff id="aff-1"><label>1</label><institution>Laboratory of Reproductive Neuroendocrinology, Department of Integrative Biology</institution>, <addr-line>University of California at Berkeley</addr-line>, <country>USA</country></aff>
+        <aff id="aff-2"><label>2</label><institution>Helen Wills Neuroscience Institute, University of California at Berkeley, Berkeley</institution>, <addr-line>CA</addr-line>, <country>USA</country></aff>
+      </contrib-group>
+      <contrib-group content-type="editors">
+        <contrib id="editor-1" contrib-type="editor">
+          <name>
+            <surname>Sorci</surname>
+            <given-names>Gabriele</given-names>
+          </name>
+        </contrib>
+      </contrib-group>
+      <pub-date pub-type="epub" date-type="pub" iso-8601-date="2013-08-15">
+        <day>15</day>
+        <month>8</month>
+        <year iso-8601-date="2013">2013</year>
+      </pub-date>
+      <volume>1</volume>
+      <elocation-id>e139</elocation-id>
+      <history>
+        <date date-type="received" iso-8601-date="2012-11-29">
+          <day>29</day>
+          <month>11</month>
+          <year iso-8601-date="2012">2012</year>
+        </date>
+        <date date-type="accepted" iso-8601-date="2013-07-31">
+          <day>31</day>
+          <month>7</month>
+          <year iso-8601-date="2013">2013</year>
+        </date>
+      </history>
+      <permissions>
+        <copyright-statement>© 2013 McGuire et al.</copyright-statement>
+        <copyright-year>2013</copyright-year>
+        <copyright-holder>McGuire et al.</copyright-holder>
+        <license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/3.0/">
+          <license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/3.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p>
+        </license>
+      </permissions>
+      <self-uri xlink:href="https://peerj.com/articles/139"/>
+      <abstract>
+        <p>The gonadotropin releasing hormone (GnRH) system in the hypothalamus is often considered the final point in integration of environmental cues as they pertain to the reproductive axis. However, cues such as stress and food availability are detectable in the plasma (as glucocorticoid and metabolic fuel fluctuations). Vertebrate gonads express glucocorticoid receptor, therefore we hypothesized that the gonads can detect and respond directly to cues of stress. We provide evidence here that, in addition to regulation by the brain, the gonads of European starlings (<italic>Sturnus vulgaris</italic>) respond directly to fluctuations in corticosterone and metabolic fuels by modulating sex steroid secretion. Using a 4-h gonad culture, we show that physiologically-relevant concentrations of corticosterone and metabolic stress (via use of the glucose utilization inhibitor 2-deoxy-D-glucose and the fatty acid oxidation inhibitor ethyl 2-mercaptoacetate (2DG/MA)) can directly decrease testosterone and estradiol secretion from luteinizing hormone and follicle-stimulating hormone (LH/FSH)-stimulated testes and ovaries. This effect is regulated seasonally. Prior to the breeding season, testes and ovaries respond to corticosterone and 2DG/MA by significantly decreasing gonadal steroid release. Within the breeding season, the testes do not respond to these cues of stress, while the ovaries respond only to corticosterone. This seasonal difference in response may be due in part to the influence of these cues of stress on gonadal neuropeptide expression: corticosterone upregulates GnIH expression in the testes while metabolic stress upregulates GnIH in the ovaries. Thus the gonads can directly respond to fluctuations in corticosterone and metabolic fuels during a time of critical importance to the onset of breeding.</p>
+      </abstract>
+      <kwd-group kwd-group-type="author">
+        <kwd>Stress</kwd>
+        <kwd>Gonadotropin inhibitory hormone (GnIH)</kwd>
+        <kwd>Corticosterone</kwd>
+        <kwd>Gonadotropin releasing hormone (GnRH)</kwd>
+        <kwd>Testes</kwd>
+        <kwd>Estradiol</kwd>
+        <kwd>Testosterone</kwd>
+        <kwd>Avian</kwd>
+        <kwd>Supplementary cues</kwd>
+        <kwd>Photoperiod</kwd>
+        <kwd>Ovary</kwd>
+      </kwd-group>
+      <funding-group>
+        <award-group id="fund-1">
+          <funding-source>NSF IOS</funding-source>
+          <award-id>0641188</award-id>
+          <award-id>0956338</award-id>
+          <award-id>1122044</award-id>
+        </award-group>
+        <award-group id="fund-2">
+          <funding-source>NSF GRFP</funding-source>
+        </award-group>
+        <funding-statement>This work was supported by grants from NSF IOS 0641188, 0956338 and 1122044 to George E. Bentley and NSF Graduate Research Fellowship Program (NLM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement>
+      </funding-group>
+    </article-meta>
+  </front>
+  <body>
+    <sec sec-type="intro">
+      <title>Introduction</title>
+      <p>Many seasonal breeders undergo seasonal cycles of gonadal growth and regression, accompanied by changes in sex steroid production and gamete maturation. These cycles are modulated primarily by photoperiod cues (<xref ref-type="bibr" rid="ref-20">Dawson et al., 2001</xref>; <xref ref-type="bibr" rid="ref-49">Nicholls, Goldsmith &amp; Dawson, 1988</xref>). Despite the strong influence of changing day length on the timing of reproduction, the precise timing of the onset of breeding can be modulated by multiple supplementary cues: food availability, temperature, weather, social cues and sustained stress (<xref ref-type="bibr" rid="ref-28">Gibb, 1950</xref>; <xref ref-type="bibr" rid="ref-67">Wingfield, 1984</xref>; <xref ref-type="bibr" rid="ref-68">Wingfield, 1985a</xref>; <xref ref-type="bibr" rid="ref-69">Wingfield, 1985b</xref>; <xref ref-type="bibr" rid="ref-70">Wingfield, Moore &amp; Farner, 1983</xref>). Traditionally, release or inhibition of the neuropeptide gonadotropin-releasing hormone (GnRH) from the hypothalamus has been considered as the final integration point of photoperiodic and supplemental information (<xref ref-type="bibr" rid="ref-4">Ball, 1993</xref>). However, the appropriate timing of reproduction and synchrony with conspecifics is so important that multiple regulatory points in the hypothalamo-pituitary-gonad (HPG) axis might well be required.</p>
+      <p>Gonadotropin inhibiting hormone (GnIH) is another hypothalamic neuropeptide that modulates the secretion of luteinizing hormone (LH) and follicle stimulating hormone (FSH) from the anterior pituitary gland; it also inhibits action of GnRH neurons directly (<xref ref-type="bibr" rid="ref-9">Bentley et al., 2006</xref>; <xref ref-type="bibr" rid="ref-10">Bentley et al., 2009</xref>; <xref ref-type="bibr" rid="ref-24">Ducret, Anderson &amp; Herbison, 2009</xref>; <xref ref-type="bibr" rid="ref-59">Tsutsui et al., 2000</xref>; <xref ref-type="bibr" rid="ref-61">Ubuka et al., 2008</xref>). Both GnRH and GnIH in the brain are altered in response to food restriction and stimulation of the hypothalamo-pituitary-adrenal (stress) axis (<xref ref-type="bibr" rid="ref-12">Bergendahl, Perheentupa &amp; Huhtaniemi, 1991</xref>; <xref ref-type="bibr" rid="ref-16">Calisi, Perfito &amp; Bentley, 2010</xref>; <xref ref-type="bibr" rid="ref-18">Ciccone, Dunn &amp; Sharp, 2007</xref>; <xref ref-type="bibr" rid="ref-29">Grieves et al., 2008</xref>; <xref ref-type="bibr" rid="ref-36">Kirby et al., 2009</xref>; <xref ref-type="bibr" rid="ref-52">Rivest, Plotsky &amp; Rivier, 1993</xref>). Recently, GnIH and GnIH receptor (GnIHR) expression were identified in the gonads of songbirds and primates, in addition to the brain (<xref ref-type="bibr" rid="ref-11">Bentley et al., 2008</xref>; <xref ref-type="bibr" rid="ref-44">McGuire &amp; Bentley, 2010b</xref>). Furthermore, GnIH was able to decrease directly and dose-dependently the amount of testosterone secreted by gonadotropin-stimulated house sparrow testes <italic>in vitro</italic> (<xref ref-type="bibr" rid="ref-43">McGuire &amp; Bentley, 2010a</xref>). The gonadal GnIH system can be modulated directly by circulating melatonin, thereby influencing gonadal steroid release independently of the brain (<xref ref-type="bibr" rid="ref-45">McGuire, Kangas &amp; Bentley, 2011</xref>). Gonadotropin releasing hormone (GnRH) and its receptor (GnRHR) are also expressed in the gonads of vertebrates and have direct and typically inhibitory effects on sex steroid synthesis in species from protochordates to mammals, also the way in which the gonadal GnRH system is modulated is less clear (<xref ref-type="bibr" rid="ref-44">McGuire &amp; Bentley, 2010b</xref>).</p>
+      <p>Vertebrate gonads are physiologically capable of detecting some stress cues. Plasma corticosterone and cortisol vary in response to stress in a season-specific manner (<xref ref-type="bibr" rid="ref-54">Romero, 2002</xref>) and receptors for glucocorticoids are present in gonadal tissues of songbirds (<xref ref-type="bibr" rid="ref-38">Kwok et al., 2007</xref>; <xref ref-type="bibr" rid="ref-39">Lattin et al., 2011</xref>) and other vertebrates (<xref ref-type="bibr" rid="ref-6">Bambino &amp; Hsueh, 1981</xref>; <xref ref-type="bibr" rid="ref-21">Denari &amp; Ceballos, 2006</xref>; <xref ref-type="bibr" rid="ref-31">Hsueh &amp; Erickson, 1978</xref>; <xref ref-type="bibr" rid="ref-38">Kwok et al., 2007</xref>). Acute stress can also decrease plasma testosterone in a wild male songbird, apparently in the absence of a reduction in plasma LH (<xref ref-type="bibr" rid="ref-23">Deviche et al., 2010</xref>), indicating a potentially direct effect of stress on the gonads. This effect can persist for up to 6 h (<xref ref-type="bibr" rid="ref-22">Deviche et al., 2012</xref>). In addition, food restriction can be detected in the plasma through fluctuations in metabolic fuels, and can have an effect on reproduction (<xref ref-type="bibr" rid="ref-58">Szymanski et al., 2007</xref>). As metabolic fuels are required for gamete and sex steroid production, it is likely that the gonads detect these fluctuations in plasma glucose and fatty acids directly in addition to fluctuations in circulating glucocorticoids.</p>
+      <p>As a result of the fact that mammalian and avian gonads express glucocorticoid receptors, and because food restriction can inhibit reproduction and sexual behavior (<xref ref-type="bibr" rid="ref-56">Schneider, 2004</xref>), we sought to determine whether gonads of European starlings (<italic>Sturnus vulgaris</italic>) decrease sex steroid secretion in direct response to exposure to glucocorticoids or reduced access to metabolic fuel, via the gonadal GnIH and GnRH systems.</p>
+    </sec>
+    <sec sec-type="methods">
+      <title>Materials and Methods</title>
+      <p>All procedures were performed in accordance with federal and state laws and with appropriate agreements from the UC Berkeley Office of Laboratory Animal Care.</p>
+      <p>European starlings (<italic>Sturnus vulgaris</italic>) housed under natural photoperiods in outdoor aviaries in Berkeley, CA were captured in mist and hand nets on February 5, 2010 (<italic>n</italic> = 6 males, 6 females) and April 7, 2010 (<italic>n</italic> = 6 males, 5 females). February 5, 2010 had a day length of 10 h 27 min and was preceded by &gt;6 weeks of natural short days. April 7, 2010 had a day length of 12 h 52 min. All birds were terminally anesthetized using isoflurane and then decapitated within 3 min of capture.</p>
+      <sec>
+        <title>Gonad culture</title>
+        <p>Gonad culture was performed on each day of capture with an identical procedure, except where noted. Testes and ovaries from each bird were removed, measured and incubated in individual tubes of Dulbecco’s modified eagle medium (DMEM; Sigma cat# D8437) for 4 h at 4°C prior to experimentation to establish basal levels of steroidogenesis and protein transcription. Then each left testis (T<sub>L</sub>) and each ovary (O) was snipped into pieces using clean dissection scissors. Hierarchical follicles, if present, were removed and not used for this experiment. The mass of each piece was recorded and then placed in to fresh culture medium containing: (1) 500 µl DMEM alone, (2) 0.25 µg/ml LH/FSH (44 LH: 1 FSH; National Hormone and Peptide Program, Torrance, CA) in DMEM, (3) 400 nM corticosterone (Sigma cat# C2505) and 0.25 µg/ml LH/FSH in DMEM, (4) 75 µg/ml 2-deoxy-D-glucose (2DG, Sigma cat# D8375) or 2.5 µg/ml ethyl 2-mercaptoacetate (MA, Spectrum Chemical Mfg. cat#E2833) and 0.25 µg/ml LH/FSH in DMEM. We hereafter refer to culture medium 1 as “basal” since it contains no treatment agents that would affect steroidogenesis. The ratio and concentration of LH/FSH we used has been previously defined as physiological and used successfully to stimulate sex steroid production (<xref ref-type="bibr" rid="ref-37">Kubokawa, Ishii &amp; Wingfield, 1994</xref>; <xref ref-type="bibr" rid="ref-43">McGuire &amp; Bentley, 2010a</xref>). Corticosterone is the native glucocorticoid in European starlings, and its plasma concentration is elevated during times of stress (<xref ref-type="bibr" rid="ref-55">Romero &amp; Remage-Healey, 2000</xref>). 2DG is a pharmacological agent which potently inhibits glucose utilization (<xref ref-type="bibr" rid="ref-63">Wick et al., 1957</xref>). MA is a pharmacological agent which prevents tissues from oxidizing fatty acids (<xref ref-type="bibr" rid="ref-7">Bauche et al., 1983</xref>; <xref ref-type="bibr" rid="ref-13">Bernard, Mioskowski &amp; Groscolas, 2002</xref>). 2DG/MA prevent the utilization of metabolic fuels, thus they were used here, in culture, to simulate the lack of available metabolic fuels experienced by European starlings under food-limited conditions. Concentration of total corticosterone used was within physiological range (<xref ref-type="bibr" rid="ref-40">Li et al., 2008</xref>; <xref ref-type="bibr" rid="ref-55">Romero &amp; Remage-Healey, 2000</xref>). 2-DG and MA are pharmacological agents (<xref ref-type="bibr" rid="ref-7">Bauche et al., 1983</xref>; <xref ref-type="bibr" rid="ref-63">Wick et al., 1957</xref>).</p>
+        <p>All cultures were placed in a sealed incubator for a 4 h, high humidity incubation at 37°C immediately after placement of tissue. 100% oxygen was pumped into the chamber at 4L/min to allow for maximum respiration of cells. A time period of four hours was chosen to simulate a stress event of longer duration than typical capture restraint. The type of chronic stress that we are referring to would be experienced during an environmental perturbation (i.e., winter storm, habitat disturbance, microclimate change, human disturbance, temperature stress or food shortage). Corticosterone levels in birds are often chronically elevated during such events and these birds may also experience restricted access to food (<xref ref-type="bibr" rid="ref-2">Astheimer, Buttemer &amp; Wingfield, 1992</xref>; <xref ref-type="bibr" rid="ref-15">Breuner &amp; Hahn, 2003</xref>; <xref ref-type="bibr" rid="ref-25">Fernandez-Juricic, 2000</xref>; <xref ref-type="bibr" rid="ref-33">Jenni-Elermann et al., 2008</xref>; <xref ref-type="bibr" rid="ref-35">Ketterson &amp; King, 1977</xref>; <xref ref-type="bibr" rid="ref-41">Lynn, Breuner &amp; Wingfield, 2003</xref>; <xref ref-type="bibr" rid="ref-46">Morton, 2002</xref>; <xref ref-type="bibr" rid="ref-64">Wingfield &amp; Ramenofsky, 1997</xref>). Although we aimed to mimic chronic stressors, the time period we chose may also be applicable to capture restraint stress paradigms as corticosterone can remain elevated for 30–180 min post-stimulus/CRS in a number of species (<xref ref-type="bibr" rid="ref-66">Wingfield, Vieck &amp; Moore, 1992</xref>). Experimentally, the elevation of corticosterone in response to chronic stress is dampened only after days to weeks of exposure to stressors, thus a 4 h stress event would not elicit this phenomenon (<xref ref-type="bibr" rid="ref-51">Rich &amp; Romero, 2005</xref>). A four hour culture is also applicable to metabolic stress, as birds have significantly reduced plasma glucose and triglycerides after a 2–12 h fast (<xref ref-type="bibr" rid="ref-34">Jenni-Elermann &amp; Jenni, 1996</xref>). The <italic>ex vivo</italic> culture was not extended beyond 8 h so as to prevent artifacts associated with tissue death.</p>
+        <p>Following incubation, cultures were removed from the incubator, placed on ice and centrifuged at 1500 g 4°C. The supernatant was pipetted to a clean tube and stored at −20°C for sex steroid analysis using ELISA. The tissues were washed once each with DMEM and 0.01 M phosphate buffered saline, then stored at −20°C prior to analysis for GnIH and GnRH expression using semi-quantitative PCR with an endogenous control.</p>
+      </sec>
+      <sec>
+        <title>Analysis of testosterone and estradiol secretion</title>
+        <p>The culture media supernatants from testes cultures were assayed for testosterone using ELISA (Cayman Chemical cat# 582701) and the culture media supernatants from ovary cultures were assayed for estradiol using ELISA (Cayman Chemical cat# 582251) according to the manufacturer’s instructions. These kits were developed specifically for use with tissue culture supernatants, and have specific instructions and general precautions for using this type of sample (see <uri xlink:href="https://www.caymanchem.com/pdfs/582701.pdf">https://www.caymanchem.com/pdfs/582701.pdf</uri> and <uri xlink:href="https://www.caymanchem.com/pdfs/582251.pdf">https://www.caymanchem.com/pdfs/582251.pdf</uri> for information on this and on specificity).</p>
+        <p>Briefly, standards and samples were incubated with testosterone or estradiol antiserum and testosterone or estradiol acetylcholinesterase tracer in mouse anti-rabbit IgG-coated microplate wells at room temperature for 2 h at 45 rpm. Blank and maximum binding (buffer only) wells were also prepared at this time. After washing, the wells were developed with Ellman’s reagent, 5, 5′-dithiobis-2-nitrobenzoic acid, in the dark for 60 min. Assays were performed in duplicate and were read at 415 nm on a microplate reader (BioRad, Model 680XR) at + 0, + 5, + 15 and + 30 min. Data were collected when maximum binding wells reached an absorbance within 0.3–1 A.U. All readings were corrected to the absorbance of the blank wells, then standards and samples were converted to percentages of maximum binding. A standard curve was drawn using log(agonist) v. response variable slope four parameter curve fit in GraphPad Prism 5.0 software. The concentrations of testosterone or estradiol in the culture media were interpolated from the appropriate standard curves.</p>
+        <p>Testosterone and estradiol concentrations from each culture were corrected to the amount of tissue in each culture. To control for differences in mass of gonad sections in each culture, testosterone and estradiol data are corrected to testes or ovary section mass measured just prior to placement in treatment cultures (pg testosterone or estradiol/mg tissue).</p>
+        <p>All samples were assayed in duplicate in a single assay. Assay characteristics were 20 pg/ml for estradiol and 6 pg/ml for testosterone. The intra-assay coefficient of variation for estradiol was 4.524 and for testosterone was 1.463.</p>
+      </sec>
+      <sec>
+        <title>Analysis of GnIH and GnRH expression</title>
+        <p>Tissues from the February 5 cultures were analyzed for the effects of treatments on relative GnIH and GnRH mRNA expression using semi-quantitative PCR with an endogenous control. This method has been previously described (<xref ref-type="bibr" rid="ref-26">Foley, Leonard &amp; Engel, 1993</xref>; <xref ref-type="bibr" rid="ref-45">McGuire, Kangas &amp; Bentley, 2011</xref>; <xref ref-type="bibr" rid="ref-57">Spencer &amp; Christensen, 1999</xref>). Briefly, RNA was isolated from tissue in each culture using TRIzol (Invitrogen cat# 15596018) according to the manufacturer’s protocol. RNA concentration, quality and contamination were assessed using NanoDrop and NanoDrop-1000 3.3.0 accessory software. RNA samples with 10 mm absorbance at wavelengths from 220–350 nm or 260/280 ratio of &gt;1.6 were not used for further analysis. 1 ug total RNA from each tissue sample was then reverse transcribed using oligo(deoxythymidine)15 primer and M-MLV Reverse Transcriptase (Promega cat #s: C1101, M1701). Partial European starling GnIH and GnRH precursors were amplified from 500 ng cDNA by PCR using primers based on European starling GnIH and GnRH precursor cDNA sequences (respective GenBank accession #s EF486798, FJ514493): GnIH forward primer 5′-GGAAGAAAAGCAGAGGAGTCTC-3′, reverse primer 5′-TGGAGATCTCCCAAGCCTGT-3′; GnRH forward primer 5′-TCTCTCAGGCAGCAGGATGGA-3′, reverse primer 5′-5′-CTTTCTTCTGCCTTGTTCCTCC-3′. β-actin was also amplified from the cDNA and used as the endogenous control. All PCR amplifications were performed using a Taq polymerase kit (TaKaRa Ex Taq™; Takara Bio Inc., Shiga, Japan). Products were run on a 1.5% agarose gel and quantified by fluorescence of ethidium bromide under a UV Transilluminator (UVP Inc., Upland, CA) using a two-dimensional image analysis of the gel in Adobe Photoshop CS2. The intensity of each of the GnIH and GnRH signals was normalized to the B-actin internal control intensity of the same bird. The relative mRNA levels of GnIH and GnRH from the tissue in each culture (expressed as 0%–100% of B-actin) were then compared.</p>
+      </sec>
+      <sec>
+        <title>Statistical analysis</title>
+        <p>Data were analyzed using one-way analysis of variance (ANOVA), followed by the post-hoc Tukey’s multiple comparison test if the ANOVA provided <italic>P</italic> &lt; 0.05.</p>
+        <p>To determine if the treatments were any more or less effective in photostimulated birds versus photosensitive birds, 2-way ANOVA was performed with time and treatment as independent variables.</p>
+      </sec>
+    </sec>
+    <sec sec-type="results">
+      <title>Results</title>
+      <p>February 5, 2010 had a day length of 10 h 27 min. This daylength is less than the critical daylength of 11.5 h required for full photostimulation (and for the onset of ensuing photorefractoriness several weeks later) and was preceded by &gt;6 weeks of short days. Birds were thus classified as photosensitive (pre-breeding) (<xref ref-type="bibr" rid="ref-14">Boulakoud &amp; Goldsmith, 1995</xref>; <xref ref-type="bibr" rid="ref-48">Nicholls et al., 1987</xref>). Testes collected from European starling males had an average volume of 76.0 +- 22.5 mm<sup>3</sup> (volume = 4/3π<italic>a</italic><sup>2</sup><italic>b</italic>, where <italic>a</italic> is half the width and <italic>b</italic> is half the length (long axis) of the testis). Ovaries collected from European starling females had an average ovarian mass of 6.2  +-  1.5 mg and all follicles were &lt;1 mm in diameter.</p>
+      <p>April 7, 2010 had a day length of 12 h 52 min, which exceeds the critical daylength of 11.5 h required for photostimulation and for the resulting onset of photorefractoriness several weeks later. Birds were thus classified as photostimulated (breeding) (<xref ref-type="bibr" rid="ref-48">Nicholls et al., 1987</xref>). Testes collected from European starling males had an average testes volume of 502.0 +- 168.9 mm<sup>3</sup> and ovaries collected from European starling females had an average ovarian mass of 30.2  +-  11.3 mg and had hierarchical follicles present.</p>
+      <p>Testosterone secretion in cultured European starling testes is significantly affected by culture treatments. However, the treatments are more effective prior to the breeding season compared to breeding birds (<xref ref-type="fig" rid="fig-1">Fig. 1A</xref>: photostimulated, one way ANOVA <italic>F</italic>(5, 3) = 3.33, <italic>p</italic> &lt; 0.048); <xref ref-type="fig" rid="fig-1">Fig. 1B</xref>: photosensitive, one way ANOVA <italic>F</italic>(3, 5) = 10.67, <italic>p</italic> = 0.0005. When either photosensitive or photostimulated, testes are able to respond to culture with LH/FSH by increasing testosterone production significantly above basal levels (breeding: <italic>p</italic> &lt; 0.05, prior to breeding: <italic>p</italic> &lt; 0.001). Post-hoc comparison showed that when photosensitive, cultured testes exhibit significantly reduced testosterone secretion in the presence of corticosterone or metabolic inhibition (via 2DG/MA) compared to testes cultured in LH/FSH alone (corticosterone: <italic>p</italic> &lt; 0.01; 2DG/MA: <italic>p</italic> &lt; 0.01). Testes collected from photostimulated birds do not show reduced testosterone secretion in response to corticosterone or metabolic inhibition. Overall, there was an effect of season on the response to treatment (2-way ANOVA <italic>F</italic>(1, 3) = 12.66, <italic>p</italic> &lt; 0.01).</p>
+      <fig id="fig-1">
+        <object-id pub-id-type="doi">10.7717/peerj.139/fig-1</object-id>
+        <label>Figure 1</label>
+        <caption>
+          <title>Seasonal differences in response to corticosterone and metabolic stress in European starling (<italic>Sturnus vulgaris</italic>) testes.</title>
+          <p>(A) In testes collected from photostimulated male starlings, testosterone secretion is significantly increased in culture by incubation with LH/FSH compared to media alone. Corticosterone and the metabolic inhibitors 2DG/MA do not attenuate LH/FSH-stimulated testosterone levels in photostimulated starlings. (B) In testes collected from photosensitive male starlings, testosterone secretion is significantly increased in culture by incubation with LH/FSH compared to media alone. Corticosterone and the metabolic inhibitors 2DG/MA significantly attenuate LH/FSH-stimulated testosterone levels. Bars are mean testosterone secreted +-  S.E.M. Different letters above columns indicate statistically significant differences.</p>
+        </caption>
+        <graphic mimetype="image" mime-subtype="png" xlink:href="https://peerj.com/articles/139/fig-1.png"/>
+      </fig>
+      <p>Estradiol secretion in cultured European starling ovaries is significantly affected by culture treatments in photosensitive (prior to breeding) and photostimulated (breeding) birds (<xref ref-type="fig" rid="fig-2">Fig. 2A</xref>: photostimulated, one way ANOVA <italic>F</italic>(3, 5) = 6.6, <italic>p</italic> = 0.0046; <xref ref-type="fig" rid="fig-2">Fig. 2B</xref>: photosensitive, one way ANOVA <italic>F</italic>(3, 5) = 7.31, <italic>p</italic> = 0.003. When photostimulated or photosensitive, ovaries are able to respond to culture with LH/FSH by increasing estradiol production significantly above basal levels (post-hoc comparison for photostimulated: <italic>p</italic> &lt; 0.01; photosensitive: <italic>p</italic> &lt; 0.01). Ovaries from photosensitive birds show significantly reduced estradiol secretion in the presence of corticosterone or metabolic inhibition (via 2DG/MA) compared to ovaries cultured in LH/FSH alone (corticosterone: <italic>p</italic> &lt; 0.05; 2DG/MA: <italic>p</italic> &lt; 0.05). Ovaries collected from photostimulated birds show reduced estradiol secretion in response to corticosterone only (corticosterone: <italic>p</italic> &lt; 0.05). Overall, there was an effect of season on the response to treatment (2-way ANOVA <italic>F</italic>(1, 3) = 7.58, <italic>p</italic> &lt; 0.02).</p>
+      <fig id="fig-2">
+        <object-id pub-id-type="doi">10.7717/peerj.139/fig-2</object-id>
+        <label>Figure 2</label>
+        <caption>
+          <title>Seasonal differences in response to corticosterone and metabolic stress in European starling (<italic>Sturnus vulgaris</italic>) ovaries.</title>
+          <p>(A) In ovaries collected from photostimulated female starlings, estradiol secretion is significantly increased in culture by incubation with LH/FSH compared to media alone. Corticosterone significantly attenuates LH/FSH-stimulated estradiol levels in photostimulated starlings. (B) In ovaries collected from photosensitive female starlings, estradiol secretion is significantly increased in culture by incubation with LH/FSH compared to media alone. Corticosterone and the metabolic inhibitors 2DG/MA significantly attenuate LH/FSH-stimulated testosterone concentrations. Bars are mean estradiol secreted +-  S.E.M. Different letters above columns indicate statistically significant differences.</p>
+        </caption>
+        <graphic mimetype="image" mime-subtype="png" xlink:href="https://peerj.com/articles/139/fig-2.png"/>
+      </fig>
+      <p>Corticosterone and the metabolic stressors 2DG/MA directly affect the expression of gonadal GnIH in gonadotropin-stimulated photosensitive European starling testes and ovaries (testes, <xref ref-type="fig" rid="fig-3">Fig. 3A</xref>: one way ANOVA <italic>F</italic>(2, 5) = 12.70, <italic>p</italic> = 0.0018; ovaries, <xref ref-type="fig" rid="fig-3">Fig. 3B</xref>: one way ANOVA <italic>F</italic>(2, 4) = 7.81, <italic>p</italic> = 0.013). In the testes, incubation with corticosterone significantly increases relative GnIH expression compared to LH/FSH-stimulated tissue (post-hoc comparison: <italic>p</italic> &lt; 0.01). In the ovaries, incubation with the metabolic inhibitors 2-DG/MA significantly increases relative GnIH expression compared to LH/FSH-stimulated tissue (<italic>p</italic> &lt; 0.05). GnIH expression did not differ between LH/FSH-treated ovarian tissue and LH/FSH+cort-treated ovarian tissue.</p>
+      <fig id="fig-3">
+        <object-id pub-id-type="doi">10.7717/peerj.139/fig-3</object-id>
+        <label>Figure 3</label>
+        <caption>
+          <title>Stressors upregulate gonadal expression of GnIH mRNA in photosensitive birds.</title>
+          <p>Expression of GnIH is significantly upregulated by corticosterone in the testes and by the metabolic inhibition via 2DG/MA in the ovary compared to LH/FSH stimulation alone. Bars are mean GnIH expressed +-  S.E.M. Different letters above columns indicate statistically significant differences.</p>
+        </caption>
+        <graphic mimetype="image" mime-subtype="png" xlink:href="https://peerj.com/articles/139/fig-3.png"/>
+      </fig>
+      <fig id="fig-4">
+        <object-id pub-id-type="doi">10.7717/peerj.139/fig-4</object-id>
+        <label>Figure 4</label>
+        <caption>
+          <title>Stressors do not affect gonadal expression of GnRH mRNA in photosensitive birds.</title>
+          <p>Expression of GnRH in the testes and ovaries is not significantly different in cultures treated with LH/FSH alone, LH/FSH +corticosterone or LH/FSH +2DG/MA. Bars are mean GnRH expressed +-  S.E.M.</p>
+        </caption>
+        <graphic mimetype="image" mime-subtype="png" xlink:href="https://peerj.com/articles/139/fig-4.png"/>
+      </fig>
+      <p>Stressors do not directly affect the expression of gonadal GnRH in gonadotropin-stimulated photosensitive European starling testes and ovaries (testes: one way ANOVA <italic>F</italic>(2, 5) = 1.00, <italic>p</italic> = 0.408; ovaries: one way ANOVA <italic>F</italic>(2, 5) = 2.794, <italic>p</italic> = 0.109). These data are shown in <xref ref-type="fig" rid="fig-4">Fig. 4</xref>.</p>
+    </sec>
+    <sec sec-type="discussion">
+      <title>Discussion</title>
+      <p>In addition to regulation from the brain, the gonads of European starlings (<italic>Sturnus vulgaris</italic>) are able to detect cues of stress directly and respond (in a season-specific manner) by modulating sex steroid secretion. The testes and ovaries of photosensitive (prior to breeding) European starlings show significantly reduced gonadotropin-stimulated testosterone and estradiol secretion when cultured with the glucocorticoid corticosterone, or with the metabolic inhibitors, 2DG/MA, compared to gonadotropin-stimulation alone. As birds become photostimulated and reach full reproductive condition, the testes no longer respond to corticosterone or 2DG/MA in culture with reduced testosterone secretion. The ovaries of photostimulated starlings do maintain their responsiveness to corticosterone at this time, but no longer respond to 2DG/MA in culture with reduced estradiol secretion.</p>
+      <p>What accounts for these seasonal and sex differences? Photosensitive birds are still making the ‘decision’ to breed, while photostimulated birds are already maximally stimulated by gonadotropins from the HPG axis. Supplementary cues (i.e., non-photic cues of the suitability of the future environment for breeding and rearing young, such as habitat quality, presence of mates, weather and stress) are much more important in the decision to delay or advance the onset of breeding, and appear to be less influential once birds have already committed to breeding (<xref ref-type="bibr" rid="ref-4">Ball, 1993</xref>; <xref ref-type="bibr" rid="ref-19">Dawson, 2008</xref>; <xref ref-type="bibr" rid="ref-30">Hinde &amp; Steel, 1976</xref>; <xref ref-type="bibr" rid="ref-65">Wingfield &amp; Sapolsky, 2003</xref>). This may be mediated physiologically in the gonads by a dampening of the responsiveness to stress hormones and metabolic stress during breeding. Additionally, recrudescence of the gonads represents a higher metabolic cost than simply maintaining large gonads once they have recrudesced (<xref ref-type="bibr" rid="ref-62">Vezina &amp; Salvante, 2010</xref>), although in a study on wild birds housed indoors with <italic>ad libitum</italic> food, testicular growth was not thought to be energy-demanding (<xref ref-type="bibr" rid="ref-17">Caro &amp; Visser, 2009</xref>). Thus when metabolic fuels are limited, the onset of gonadal growth (which would occur in photosensitive birds) is delayed, but maintenance of gonads (which would occur in photostimulated birds) is not. Interestingly, ovaries do continue to respond to corticosterone by locally reducing sex steroid secretion even while in breeding condition, while testes do not. This result might reflect the differing breeding strategies of males and females. Female songbirds do not initiate full follicle growth until conditions are favorable for laying (<xref ref-type="bibr" rid="ref-46">Morton, 2002</xref>). However, males will continue to support testosterone-stimulated behaviors/physiology even while stressed, so that fertilization opportunities are not lost (<xref ref-type="bibr" rid="ref-5">Ball &amp; Ketterson, 2008</xref>; <xref ref-type="bibr" rid="ref-8">Beletsky, Orians &amp; Wingfield, 1989</xref>).</p>
+      <p>Our data indicate that gonadal GnIH may be involved in mediating the season- and sex-specific responses to cues of stress. GnIH has been previously identified as a local inhibitor of testosterone production (<xref ref-type="bibr" rid="ref-43">McGuire &amp; Bentley, 2010a</xref>) and GnIH is upregulated in the testes of photosensitive males in response to corticosterone and in the ovaries of photosensitive females in response to metabolic stress (via 2DG/MA). Thus the gonads are able to respond to cues of stress directly by up-regulating this hormone. In this way, the gonads of birds are able to fine-tune their steroid secretion and possibly optimize the onset and degree of gonadal recrudescence in response to their local environment. Alternatively, other hormones may be involved. An obvious difference between breeding and non-breeding birds is exposure level to circulating gonadotropins. LH and FSH may play a role in decreasing the responsiveness of the gonads to corticosterone and metabolic stress. Additionally, prior to the breeding season, photosensitive birds experience shorter days than photostimulated birds. Light cues are normally mediated through the brain and affect the gonads via hormones of the HPG axis (<xref ref-type="bibr" rid="ref-20">Dawson et al., 2001</xref>). However, it is possible the gonads can respond directly to cues of day length. Melatonin, a hormone secreted by the pineal gland at night (thus providing a proxy of day length), is detectable in the plasma in the same phase and duration as the brain (<xref ref-type="bibr" rid="ref-42">Maywood et al., 1993</xref>; <xref ref-type="bibr" rid="ref-53">Rollag, O’Callaghan &amp; Niswender, 1978</xref>). Melatonin receptors are expressed in the gonads of birds and mammals, and melatonin appears to affect directly the physiological actions of the gonads (<xref ref-type="bibr" rid="ref-1">Ahmad &amp; Haldar, 2010</xref>; <xref ref-type="bibr" rid="ref-3">Ayre &amp; Pang, 1994</xref>; <xref ref-type="bibr" rid="ref-27">Frungieri et al., 2005</xref>; <xref ref-type="bibr" rid="ref-47">Murayama et al., 1997</xref>; <xref ref-type="bibr" rid="ref-50">Niedziela, Lerchl &amp; Nieschlag, 1995</xref>). Melatonin may thus also play a role in the regulation of seasonal responsiveness to stress in the gonads. Additionally, glucocorticoid receptor may be seasonally regulated in the gonads of birds, much like amphibians (<xref ref-type="bibr" rid="ref-21">Denari &amp; Ceballos, 2006</xref>). Alternatively, avian gonads may respond to stress through an additional pathway <italic>in vivo</italic> that circumvents the HPG axis: the neural brain-testicular pathway identified in rats (<xref ref-type="bibr" rid="ref-32">James, Rivier &amp; Lee, 2008</xref>). The existence and seasonal regulation of this system is as yet unknown in birds and requires further investigation. The possible use of seasonal cycles of LH/FSH, melatonin or glucocorticoid receptors, however, does not exclude the use of seasonal cycles of gonadal GnIH. In fact, GnIH in the hypothalamus is upregulated independently by both melatonin and stress, and GnIH neurons express glucocorticoid receptors and melatonin receptors (<xref ref-type="bibr" rid="ref-16">Calisi, Perfito &amp; Bentley, 2010</xref>; <xref ref-type="bibr" rid="ref-60">Ubuka et al., 2005</xref>). Thus there is a precedent for melatonin and corticosterone to influence GnIH directly.</p>
+      <p>The regulation and action of gonadal GnRH in songbirds requires further determination. However, we conclude here that GnRH mRNA is not upregulated in the gonads of photosensitive European starlings by the stress hormone, corticosterone, nor by the metabolic inhibitors, 2DG/MA.</p>
+      <p>In sum, the evidence provided by our model shows that the testes and ovaries of European starlings respond directly to chronically elevated corticosterone and metabolic stress independently of the brain by modulating testosterone and estradiol secretion. This modulation is season- and sex-specific: it occurs only while birds are deciding to breed, and it appears to involve the gonadal GnIH system. It is likely that this direct modulation of gonadal function in response to stress is most important prior to the onset of breeding because the gonads are not already maximally stimulated by components of the HPG axis and fully recrudesced. Taken together, our data indicate that photosensitive temperate songbird testes and ovaries are capable of responding directly to corticosterone and metabolic stress, in addition to GnRH-induced gonadotropin release, by increasing GnIH expression and decreasing testosterone and estradiol secretion. Thus, we suggest that the GnRH system is not the only pathway for integration of cues into a gonadal response.</p>
+    </sec>
+  </body>
+  <back>
+    <sec sec-type="additional-information">
+      <title>Additional Information and Declarations</title>
+      <fn-group content-type="competing-interests">
+        <title>Competing Interests</title>
+        <fn id="conflict-1" fn-type="con">
+          <p>George Bentley is an Academic Editor for PeerJ.</p>
+        </fn>
+      </fn-group>
+      <fn-group content-type="author-contributions">
+        <title>Author Contributions</title>
+        <fn id="contribution-1" fn-type="con">
+          <p><xref ref-type="contrib" rid="author-1">Nicolette L. McGuire</xref> conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper.</p>
+        </fn>
+        <fn id="contribution-2" fn-type="con">
+          <p><xref ref-type="contrib" rid="author-2">Annie Koh</xref> performed the experiments, analyzed the data.</p>
+        </fn>
+        <fn id="contribution-3" fn-type="con">
+          <p><xref ref-type="contrib" rid="author-3">George E. Bentley</xref> conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, wrote the paper.</p>
+        </fn>
+      </fn-group>
+      <fn-group content-type="other">
+        <title>Animal Ethics</title>
+        <fn id="addinfo-1" fn-type="other">
+          <p>The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers).</p>
+          <p>All procedures were performed in accordance with federal and state laws and with appropriate written agreements from the UC Berkeley Animal Care and Use Committee (ACUC).</p>
+        </fn>
+      </fn-group>
+    </sec>
+    <ref-list content-type="authoryear">
+      <title>References</title>
+      <ref id="ref-1">
+        <label>Ahmad &amp; Haldar (2010)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Ahmad</surname>
+              <given-names>R</given-names>
+            </name>
+            <name>
+              <surname>Haldar</surname>
+              <given-names>C</given-names>
+            </name>
+          </person-group>
+          <article-title>Effect of intra-testicular melatonin injection on testicular functions, local and general immunity of a tropical rodent <italic>Funambulus pennanti</italic></article-title>
+          <source>Endocrine</source>
+          <year iso-8601-date="2010">2010</year>
+          <volume>37</volume>
+          <fpage>479</fpage>
+          <lpage>488</lpage>
+          <pub-id pub-id-type="doi">10.1007/s12020-010-9331-7</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-2">
+        <label>Astheimer, Buttemer &amp; Wingfield (1992)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Astheimer</surname>
+              <given-names>L</given-names>
+            </name>
+            <name>
+              <surname>Buttemer</surname>
+              <given-names>W</given-names>
+            </name>
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>J</given-names>
+            </name>
+          </person-group>
+          <article-title>Interactions of corticosterone with feeding, activity and metabolism in passerine birds</article-title>
+          <source>Ornis Scandinavica</source>
+          <year iso-8601-date="1992">1992</year>
+          <volume>23</volume>
+          <fpage>355</fpage>
+          <lpage>365</lpage>
+          <pub-id pub-id-type="doi">10.2307/3676661</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-3">
+        <label>Ayre &amp; Pang (1994)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Ayre</surname>
+              <given-names>E</given-names>
+            </name>
+            <name>
+              <surname>Pang</surname>
+              <given-names>S</given-names>
+            </name>
+          </person-group>
+          <article-title>2-[<sup>125</sup>I]Iodomelatonin binding sites in the testis and ovary: putative melatonin receptors in the gonads</article-title>
+          <source>Biological Signals</source>
+          <year iso-8601-date="1994">1994</year>
+          <volume>3</volume>
+          <fpage>71</fpage>
+          <lpage>84</lpage>
+          <pub-id pub-id-type="doi">10.1159/000109528</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-4">
+        <label>Ball (1993)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Ball</surname>
+              <given-names>G</given-names>
+            </name>
+          </person-group>
+          <article-title>The neural integration of environmental information by seasonally breeding birds</article-title>
+          <source>American Zoologist</source>
+          <issue>2</issue>
+          <year iso-8601-date="1993">1993</year>
+          <volume>33</volume>
+          <fpage>185</fpage>
+          <lpage>199</lpage>
+          <pub-id pub-id-type="doi">10.1093/icb/33.2.185</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-5">
+        <label>Ball &amp; Ketterson (2008)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Ball</surname>
+              <given-names>G</given-names>
+            </name>
+            <name>
+              <surname>Ketterson</surname>
+              <given-names>E</given-names>
+            </name>
+          </person-group>
+          <article-title>Sex differences in the response to environmental cues regulating seasonal reproduction in birds</article-title>
+          <source>Philosophical Transactions of the Royal Society B</source>
+          <year iso-8601-date="2008">2008</year>
+          <volume>363</volume>
+          <fpage>231</fpage>
+          <lpage>246</lpage>
+          <pub-id pub-id-type="doi">10.1098/rstb.2007.2137</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-6">
+        <label>Bambino &amp; Hsueh (1981)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Bambino</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>Hsueh</surname>
+              <given-names>A</given-names>
+            </name>
+          </person-group>
+          <article-title>Direct inhibitory effect of glucocorticoids upon testicular luteinizing-hormone receptor and steroidogenesis <italic>in vivo</italic> and <italic>in vitro</italic></article-title>
+          <source>Endocrinology</source>
+          <year iso-8601-date="1981">1981</year>
+          <volume>108</volume>
+          <fpage>2142</fpage>
+          <lpage>2148</lpage>
+          <pub-id pub-id-type="doi">10.1210/endo-108-6-2142</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-7">
+        <label>Bauche et al. (1983)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Bauche</surname>
+              <given-names>F</given-names>
+            </name>
+            <name>
+              <surname>Sabourault</surname>
+              <given-names>D</given-names>
+            </name>
+            <name>
+              <surname>Guidicelli</surname>
+              <given-names>Y</given-names>
+            </name>
+            <name>
+              <surname>Nordmann</surname>
+              <given-names>J</given-names>
+            </name>
+            <name>
+              <surname>Nordmann</surname>
+              <given-names>R</given-names>
+            </name>
+          </person-group>
+          <article-title>Inhibition in vitro of acyl-CoA-dehydrogenases by 2-mercaptoacetate in rat liver mitochondria</article-title>
+          <source>Biochemical Journal</source>
+          <year iso-8601-date="1983">1983</year>
+          <volume>215</volume>
+          <fpage>457</fpage>
+          <lpage>464</lpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-8">
+        <label>Beletsky, Orians &amp; Wingfield (1989)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Beletsky</surname>
+              <given-names>L</given-names>
+            </name>
+            <name>
+              <surname>Orians</surname>
+              <given-names>G</given-names>
+            </name>
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>J</given-names>
+            </name>
+          </person-group>
+          <article-title>Relationships of steroid hormones and polygyny to territorial status, breeding experience, and reproductive success in male red-winged blackbirds</article-title>
+          <source>Auk</source>
+          <year iso-8601-date="1989">1989</year>
+          <volume>106</volume>
+          <fpage>107</fpage>
+          <lpage>117</lpage>
+          <pub-id pub-id-type="doi">10.2307/4087763</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-9">
+        <label>Bentley et al. (2006)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Bentley</surname>
+              <given-names>G</given-names>
+            </name>
+            <name>
+              <surname>Kriegsfeld</surname>
+              <given-names>L</given-names>
+            </name>
+            <name>
+              <surname>Osugi</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>Ukena</surname>
+              <given-names>K</given-names>
+            </name>
+            <name>
+              <surname>O’Brien</surname>
+              <given-names>S</given-names>
+            </name>
+            <name>
+              <surname>Perfito</surname>
+              <given-names>N</given-names>
+            </name>
+            <name>
+              <surname>Moore</surname>
+              <given-names>I</given-names>
+            </name>
+            <name>
+              <surname>Tsutsui</surname>
+              <given-names>K</given-names>
+            </name>
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>J</given-names>
+            </name>
+          </person-group>
+          <article-title>Interactions of gonadotropin-releasing hormone (GnRH) and gonadotropin-inhibitory hormone (GnIH) in birds and mammals</article-title>
+          <source>Journal of Experimental Zoology A Comparative Experimental Biology</source>
+          <year iso-8601-date="2006">2006</year>
+          <volume>305</volume>
+          <fpage>807</fpage>
+          <lpage>814</lpage>
+          <pub-id pub-id-type="doi">10.1002/jez.a.306</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-10">
+        <label>Bentley et al. (2009)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Bentley</surname>
+              <given-names>G</given-names>
+            </name>
+            <name>
+              <surname>Ubuka</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>McGuire</surname>
+              <given-names>N</given-names>
+            </name>
+            <name>
+              <surname>Calisi</surname>
+              <given-names>R</given-names>
+            </name>
+            <name>
+              <surname>Perfito</surname>
+              <given-names>N</given-names>
+            </name>
+            <name>
+              <surname>Kriegsfeld</surname>
+              <given-names>L</given-names>
+            </name>
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>J</given-names>
+            </name>
+            <name>
+              <surname>Tsutsui</surname>
+              <given-names>K</given-names>
+            </name>
+          </person-group>
+          <article-title>Gonadotrophin-inhibitory hormone: a multifunctional neuropeptide</article-title>
+          <source>Journal of Neuroendocrinology</source>
+          <year iso-8601-date="2009">2009</year>
+          <volume>21</volume>
+          <fpage>276</fpage>
+          <lpage>281</lpage>
+          <pub-id pub-id-type="doi">10.1111/j.1365-2826.2009.01851.x</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-11">
+        <label>Bentley et al. (2008)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Bentley</surname>
+              <given-names>G</given-names>
+            </name>
+            <name>
+              <surname>Ubuka</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>McGuire</surname>
+              <given-names>N</given-names>
+            </name>
+            <name>
+              <surname>Chowdhury</surname>
+              <given-names>V</given-names>
+            </name>
+            <name>
+              <surname>Morita</surname>
+              <given-names>Y</given-names>
+            </name>
+            <name>
+              <surname>Yano</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>Haunuma</surname>
+              <given-names>I</given-names>
+            </name>
+            <name>
+              <surname>BInns</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>J</given-names>
+            </name>
+            <name>
+              <surname>Tsutsui</surname>
+              <given-names>K</given-names>
+            </name>
+          </person-group>
+          <article-title>Gonadotropin-inhibitory hormone and its receptor in the avian reproductive system</article-title>
+          <source>General and Comparative Endocrinology</source>
+          <year iso-8601-date="2008">2008</year>
+          <volume>156</volume>
+          <fpage>34</fpage>
+          <lpage>43</lpage>
+          <pub-id pub-id-type="doi">10.1016/j.ygcen.2007.10.003</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-12">
+        <label>Bergendahl, Perheentupa &amp; Huhtaniemi (1991)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Bergendahl</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Perheentupa</surname>
+              <given-names>A</given-names>
+            </name>
+            <name>
+              <surname>Huhtaniemi</surname>
+              <given-names>I</given-names>
+            </name>
+          </person-group>
+          <article-title>Starvation-induced suppression of pituitary-testicular function in rats is reversed by pulsatile gonadotropin-releasing hormone substitution</article-title>
+          <source>Biology of Reproduction</source>
+          <year iso-8601-date="1991">1991</year>
+          <volume>44</volume>
+          <fpage>413</fpage>
+          <lpage>419</lpage>
+          <pub-id pub-id-type="doi">10.1095/biolreprod44.3.413</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-13">
+        <label>Bernard, Mioskowski &amp; Groscolas (2002)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Bernard</surname>
+              <given-names>S</given-names>
+            </name>
+            <name>
+              <surname>Mioskowski</surname>
+              <given-names>E</given-names>
+            </name>
+            <name>
+              <surname>Groscolas</surname>
+              <given-names>R</given-names>
+            </name>
+          </person-group>
+          <article-title>Blockade of fatty acid oxidation mimics phase II-phase III transition in a fasting bird, the king penguin</article-title>
+          <source>American Journal of Physiology: Regulatory Integrative and Comparative Physiology</source>
+          <year iso-8601-date="2002">2002</year>
+          <volume>283</volume>
+          <fpage>R144</fpage>
+          <lpage>R152</lpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-14">
+        <label>Boulakoud &amp; Goldsmith (1995)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Boulakoud</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Goldsmith</surname>
+              <given-names>A</given-names>
+            </name>
+          </person-group>
+          <article-title>The effect of duration of exposure to short days on the gonadal response to long days in male starlings (<italic>Sturnus vulgaris</italic>)</article-title>
+          <source>Journal of Reproduction and Fertility</source>
+          <year iso-8601-date="1995">1995</year>
+          <volume>104</volume>
+          <fpage>215</fpage>
+          <lpage>217</lpage>
+          <pub-id pub-id-type="doi">10.1530/jrf.0.1040215</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-15">
+        <label>Breuner &amp; Hahn (2003)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Breuner</surname>
+              <given-names>C</given-names>
+            </name>
+            <name>
+              <surname>Hahn</surname>
+              <given-names>T</given-names>
+            </name>
+          </person-group>
+          <article-title>Integrating stress physiology, environmental change, and behavior in free-living sparrows</article-title>
+          <source>Hormones and Behavior</source>
+          <year iso-8601-date="2003">2003</year>
+          <volume>43</volume>
+          <fpage>115</fpage>
+          <lpage>123</lpage>
+          <pub-id pub-id-type="doi">10.1016/S0018-506X(02)00020-X</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-16">
+        <label>Calisi, Perfito &amp; Bentley (2010)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Calisi</surname>
+              <given-names>R</given-names>
+            </name>
+            <name>
+              <surname>Perfito</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Bentley</surname>
+              <given-names>G</given-names>
+            </name>
+          </person-group>
+          <article-title>How can stress affect the neural control of reproduction? An examination of gonadotropin inhibitory hormone (GnIH) and glucocorticoid receptors (GR) in songbirds</article-title>
+          <source>Integrative and Comparative Biology</source>
+          <year iso-8601-date="2010">2010</year>
+          <volume>50</volume>
+          <fpage>E23</fpage>
+          <lpage>E23</lpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-17">
+        <label>Caro &amp; Visser (2009)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Caro</surname>
+              <given-names>SP</given-names>
+            </name>
+            <name>
+              <surname>Visser</surname>
+              <given-names>ME</given-names>
+            </name>
+          </person-group>
+          <article-title>Temperature-induced elevation of basal metabolic rate does not affect testis growth in great tits</article-title>
+          <source>Journal of Experimental Biology</source>
+          <year iso-8601-date="2009">2009</year>
+          <volume>212</volume>
+          <fpage>1995</fpage>
+          <lpage>1999</lpage>
+          <pub-id pub-id-type="doi">10.1242/jeb.026344</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-18">
+        <label>Ciccone, Dunn &amp; Sharp (2007)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Ciccone</surname>
+              <given-names>N</given-names>
+            </name>
+            <name>
+              <surname>Dunn</surname>
+              <given-names>I</given-names>
+            </name>
+            <name>
+              <surname>Sharp</surname>
+              <given-names>P</given-names>
+            </name>
+          </person-group>
+          <article-title>Increased food intake stimulates GnRH-L glycoprotein hormone alpha-subunit and follistatin mRNAs, and ovarian follicular numbers in laying broiler breeder hens</article-title>
+          <source>Domestic Animal Endocrinology</source>
+          <year iso-8601-date="2007">2007</year>
+          <volume>33</volume>
+          <fpage>62</fpage>
+          <lpage>76</lpage>
+          <pub-id pub-id-type="doi">10.1016/j.domaniend.2006.04.008</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-19">
+        <label>Dawson (2008)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Dawson</surname>
+              <given-names>A</given-names>
+            </name>
+          </person-group>
+          <article-title>Control of the annual cycle in birds: endocrine constraints and plasticity in response to ecological variability</article-title>
+          <source>Philosophical Transactions of the Royal Society of London B Biological Sciences</source>
+          <year iso-8601-date="2008">2008</year>
+          <volume>363</volume>
+          <fpage>1621</fpage>
+          <lpage>1633</lpage>
+          <pub-id pub-id-type="doi">10.1098/rstb.2007.0004</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-20">
+        <label>Dawson et al. (2001)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Dawson</surname>
+              <given-names>A</given-names>
+            </name>
+            <name>
+              <surname>King</surname>
+              <given-names>VM</given-names>
+            </name>
+            <name>
+              <surname>Bentley</surname>
+              <given-names>GE</given-names>
+            </name>
+            <name>
+              <surname>Ball</surname>
+              <given-names>GF</given-names>
+            </name>
+          </person-group>
+          <article-title>Photoperiodic control of seasonality in birds</article-title>
+          <source>Journal of Biological Rhythms</source>
+          <year iso-8601-date="2001">2001</year>
+          <volume>16</volume>
+          <fpage>365</fpage>
+          <lpage>380</lpage>
+          <pub-id pub-id-type="doi">10.1177/074873001129002079</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-21">
+        <label>Denari &amp; Ceballos (2006)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Denari</surname>
+              <given-names>D</given-names>
+            </name>
+            <name>
+              <surname>Ceballos</surname>
+              <given-names>N</given-names>
+            </name>
+          </person-group>
+          <article-title>Cytosolic glucocorticoid receptor in the testis of <italic>Bufo arenarum</italic>: seasonal changes in its binding parameters</article-title>
+          <source>General and Comparative Endocrinology</source>
+          <year iso-8601-date="2006">2006</year>
+          <volume>147</volume>
+          <fpage>247</fpage>
+          <lpage>254</lpage>
+          <pub-id pub-id-type="doi">10.1016/j.ygcen.2006.01.006</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-22">
+        <label>Deviche et al. (2012)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Deviche</surname>
+              <given-names>P</given-names>
+            </name>
+            <name>
+              <surname>Gao</surname>
+              <given-names>S</given-names>
+            </name>
+            <name>
+              <surname>Davies</surname>
+              <given-names>S</given-names>
+            </name>
+            <name>
+              <surname>Sharp</surname>
+              <given-names>PJ</given-names>
+            </name>
+            <name>
+              <surname>Dawson</surname>
+              <given-names>A</given-names>
+            </name>
+          </person-group>
+          <article-title>Rapid stress-induced inhibition of plasma testosterone in free-ranging male rufous-winged sparrows, <italic>Peucaea carpalis</italic>: characterization, time course, and recovery</article-title>
+          <source>General and Comparative Endocrinology</source>
+          <year iso-8601-date="2012">2012</year>
+          <volume>177</volume>
+          <fpage>1</fpage>
+          <lpage>8</lpage>
+          <pub-id pub-id-type="doi">10.1016/j.ygcen.2012.02.022</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-23">
+        <label>Deviche et al. (2010)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Deviche</surname>
+              <given-names>PJ</given-names>
+            </name>
+            <name>
+              <surname>Hurley</surname>
+              <given-names>LL</given-names>
+            </name>
+            <name>
+              <surname>Fokidis</surname>
+              <given-names>HB</given-names>
+            </name>
+            <name>
+              <surname>Lerbour</surname>
+              <given-names>B</given-names>
+            </name>
+            <name>
+              <surname>Silverin</surname>
+              <given-names>B</given-names>
+            </name>
+            <name>
+              <surname>Sabo</surname>
+              <given-names>J</given-names>
+            </name>
+            <name>
+              <surname>Sharp</surname>
+              <given-names>PJ</given-names>
+            </name>
+          </person-group>
+          <article-title>Acute stress rapidly decreases plasma testosterone in a free-ranging male songbird: potential site of action and mechanism</article-title>
+          <source>General and Comparative Endocrinology</source>
+          <year iso-8601-date="2010">2010</year>
+          <volume>169</volume>
+          <fpage>82</fpage>
+          <lpage>90</lpage>
+          <pub-id pub-id-type="doi">10.1016/j.ygcen.2010.07.009</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-24">
+        <label>Ducret, Anderson &amp; Herbison (2009)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Ducret</surname>
+              <given-names>E</given-names>
+            </name>
+            <name>
+              <surname>Anderson</surname>
+              <given-names>G</given-names>
+            </name>
+            <name>
+              <surname>Herbison</surname>
+              <given-names>A</given-names>
+            </name>
+          </person-group>
+          <article-title>RFamide-related peptide-3, a mammalian gonadotropin-inhibitory hormone ortholog, regulates gonadotropin-releasing hormone neuron firing in the mouse</article-title>
+          <source>Endocrinology</source>
+          <year iso-8601-date="2009">2009</year>
+          <volume>150</volume>
+          <fpage>2799</fpage>
+          <lpage>2804</lpage>
+          <pub-id pub-id-type="doi">10.1210/en.2008-1623</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-25">
+        <label>Fernandez-Juricic (2000)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Fernandez-Juricic</surname>
+              <given-names>E</given-names>
+            </name>
+          </person-group>
+          <article-title>Local and regional effects of pedestrians on forest birds in a fragmented landscape</article-title>
+          <source>Condor</source>
+          <year iso-8601-date="2000">2000</year>
+          <volume>102</volume>
+          <fpage>247</fpage>
+          <lpage>255</lpage>
+          <pub-id pub-id-type="doi">10.1650/0010-5422(2000)102[0247:LAREOP]2.0.CO;2</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-26">
+        <label>Foley, Leonard &amp; Engel (1993)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Foley</surname>
+              <given-names>K</given-names>
+            </name>
+            <name>
+              <surname>Leonard</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Engel</surname>
+              <given-names>J</given-names>
+            </name>
+          </person-group>
+          <article-title>Quantitation of RNA using the polymerase chain reaction</article-title>
+          <source>Trends in Genetics</source>
+          <year iso-8601-date="1993">1993</year>
+          <volume>9</volume>
+          <fpage>380</fpage>
+          <lpage>385</lpage>
+          <pub-id pub-id-type="doi">10.1016/0168-9525(93)90137-7</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-27">
+        <label>Frungieri et al. (2005)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Frungieri</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Mayerhofer</surname>
+              <given-names>A</given-names>
+            </name>
+            <name>
+              <surname>Zitta</surname>
+              <given-names>K</given-names>
+            </name>
+            <name>
+              <surname>Pignataro</surname>
+              <given-names>O</given-names>
+            </name>
+            <name>
+              <surname>Calandra</surname>
+              <given-names>R</given-names>
+            </name>
+            <name>
+              <surname>Gonzalez-Calvar</surname>
+              <given-names>S</given-names>
+            </name>
+          </person-group>
+          <article-title>Direct effect of melatonin on Syrian hamster testes: melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system</article-title>
+          <source>Endocrinology</source>
+          <year iso-8601-date="2005">2005</year>
+          <volume>146</volume>
+          <fpage>1541</fpage>
+          <lpage>1552</lpage>
+          <pub-id pub-id-type="doi">10.1210/en.2004-0990</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-28">
+        <label>Gibb (1950)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Gibb</surname>
+              <given-names>J</given-names>
+            </name>
+          </person-group>
+          <article-title>The breeding biology of the great and blue titmice</article-title>
+          <source>Ibis</source>
+          <year iso-8601-date="1950">1950</year>
+          <volume>92</volume>
+          <fpage>507</fpage>
+          <lpage>539</lpage>
+          <pub-id pub-id-type="doi">10.1111/j.1474-919X.1950.tb01759.x</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-29">
+        <label>Grieves et al. (2008)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Grieves</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>Kriegsfeld</surname>
+              <given-names>L</given-names>
+            </name>
+            <name>
+              <surname>Bentley</surname>
+              <given-names>G</given-names>
+            </name>
+            <name>
+              <surname>Tsutsui</surname>
+              <given-names>K</given-names>
+            </name>
+            <name>
+              <surname>Demas</surname>
+              <given-names>G</given-names>
+            </name>
+          </person-group>
+          <article-title>Recent advances in reproductive neuroendocrinology: a role for RFamide peptides in seasonal reproduction?</article-title>
+          <source>Proceedings of the Royal Society B Biological Sciences</source>
+          <year iso-8601-date="2008">2008</year>
+          <volume>275</volume>
+          <fpage>1943</fpage>
+          <lpage>1951</lpage>
+          <pub-id pub-id-type="doi">10.1098/rspb.2008.0433</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-30">
+        <label>Hinde &amp; Steel (1976)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Hinde</surname>
+              <given-names>R</given-names>
+            </name>
+            <name>
+              <surname>Steel</surname>
+              <given-names>E</given-names>
+            </name>
+          </person-group>
+          <article-title>Effect of male song on an estrogen-dependent behavior pattern in female canary (<italic>Serinus canarius</italic>)</article-title>
+          <source>Hormones and Behavior</source>
+          <year iso-8601-date="1976">1976</year>
+          <volume>7</volume>
+          <fpage>293</fpage>
+          <lpage>304</lpage>
+          <pub-id pub-id-type="doi">10.1016/0018-506X(76)90035-0</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-31">
+        <label>Hsueh &amp; Erickson (1978)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Hsueh</surname>
+              <given-names>A</given-names>
+            </name>
+            <name>
+              <surname>Erickson</surname>
+              <given-names>G</given-names>
+            </name>
+          </person-group>
+          <article-title>Glucocorticoid inhibition of FSH-induced estrogen production in cultured rat granulosa cells</article-title>
+          <source>Steroids</source>
+          <year iso-8601-date="1978">1978</year>
+          <volume>32</volume>
+          <fpage>639</fpage>
+          <lpage>648</lpage>
+          <pub-id pub-id-type="doi">10.1016/0039-128X(78)90074-0</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-32">
+        <label>James, Rivier &amp; Lee (2008)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>James</surname>
+              <given-names>P</given-names>
+            </name>
+            <name>
+              <surname>Rivier</surname>
+              <given-names>C</given-names>
+            </name>
+            <name>
+              <surname>Lee</surname>
+              <given-names>S</given-names>
+            </name>
+          </person-group>
+          <article-title>Presence of corticotrophin-releasing factor and/or tyrosine hydroxylase in cells of a neural brain-testicular pathway that are labelled by a transganglionic tracer</article-title>
+          <source>Journal of Neuroendocrinology</source>
+          <year iso-8601-date="2008">2008</year>
+          <volume>20</volume>
+          <fpage>173</fpage>
+          <lpage>181</lpage>
+          <pub-id pub-id-type="doi">10.1111/j.1365-2826.2007.01630.x</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-33">
+        <label>Jenni-Elermann et al. (2008)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Jenni-Elermann</surname>
+              <given-names>S</given-names>
+            </name>
+            <name>
+              <surname>Glaus</surname>
+              <given-names>E</given-names>
+            </name>
+            <name>
+              <surname>Gruebler</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Schwabl</surname>
+              <given-names>H</given-names>
+            </name>
+            <name>
+              <surname>Jenni</surname>
+              <given-names>L</given-names>
+            </name>
+          </person-group>
+          <article-title>Glucocorticoid response to food availability in breeding barn swallows (<italic>Hirundo rustica</italic>)</article-title>
+          <source>General and Comparative Endocrinology</source>
+          <year iso-8601-date="2008">2008</year>
+          <volume>155</volume>
+          <fpage>558</fpage>
+          <lpage>565</lpage>
+          <pub-id pub-id-type="doi">10.1016/j.ygcen.2007.08.011</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-34">
+        <label>Jenni-Elermann &amp; Jenni (1996)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Jenni-Elermann</surname>
+              <given-names>S</given-names>
+            </name>
+            <name>
+              <surname>Jenni</surname>
+              <given-names>L</given-names>
+            </name>
+          </person-group>
+          <article-title>Metabolic differences between the postbreeding, moulting and migratory periods in feeding and fasting passerine birds</article-title>
+          <source>Functional Ecology</source>
+          <year iso-8601-date="1996">1996</year>
+          <volume>10</volume>
+          <fpage>62</fpage>
+          <lpage>72</lpage>
+          <pub-id pub-id-type="doi">10.2307/2390263</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-35">
+        <label>Ketterson &amp; King (1977)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Ketterson</surname>
+              <given-names>E</given-names>
+            </name>
+            <name>
+              <surname>King</surname>
+              <given-names>J</given-names>
+            </name>
+          </person-group>
+          <article-title>Metabolic and behavioral responses to fasting in the white-crowned sparrow (<italic>Zonotrichia leucophrys gambelii</italic>)</article-title>
+          <source>Physiological Zoology</source>
+          <year iso-8601-date="1977">1977</year>
+          <volume>50</volume>
+          <fpage>115</fpage>
+          <lpage>129</lpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-36">
+        <label>Kirby et al. (2009)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Kirby</surname>
+              <given-names>E</given-names>
+            </name>
+            <name>
+              <surname>Geraghty</surname>
+              <given-names>A</given-names>
+            </name>
+            <name>
+              <surname>Ubuka</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>Bentley</surname>
+              <given-names>G</given-names>
+            </name>
+            <name>
+              <surname>Kaufer</surname>
+              <given-names>D</given-names>
+            </name>
+          </person-group>
+          <article-title>Stress increases putative gonadotropin inhibitory hormone and decreases luteinizing hormone in male rats</article-title>
+          <source>Proceedings of the National Academy of Science USA</source>
+          <year iso-8601-date="2009">2009</year>
+          <volume>106</volume>
+          <fpage>11324</fpage>
+          <lpage>11329</lpage>
+          <pub-id pub-id-type="doi">10.1073/pnas.0901176106</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-37">
+        <label>Kubokawa, Ishii &amp; Wingfield (1994)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Kubokawa</surname>
+              <given-names>K</given-names>
+            </name>
+            <name>
+              <surname>Ishii</surname>
+              <given-names>S</given-names>
+            </name>
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>J</given-names>
+            </name>
+          </person-group>
+          <article-title>Effect of day length on luteinizing hormone beta-subunit mRNA and subsequent gonadal growth in the white-crowned sparrow, <italic>Zonotrichia leucophrys gambelii</italic></article-title>
+          <source>General and Comparative Endocrinology</source>
+          <year iso-8601-date="1994">1994</year>
+          <volume>95</volume>
+          <fpage>42</fpage>
+          <lpage>51</lpage>
+          <pub-id pub-id-type="doi">10.1006/gcen.1994.1100</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-38">
+        <label>Kwok et al. (2007)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Kwok</surname>
+              <given-names>A</given-names>
+            </name>
+            <name>
+              <surname>Wang</surname>
+              <given-names>Y</given-names>
+            </name>
+            <name>
+              <surname>Wang</surname>
+              <given-names>C</given-names>
+            </name>
+            <name>
+              <surname>Leung</surname>
+              <given-names>F</given-names>
+            </name>
+          </person-group>
+          <article-title>Cloning of chicken glucocorticoid receptor (GR) and characterization of its expression in pituitary and extrapituitary tissues</article-title>
+          <source>Poultry Science</source>
+          <year iso-8601-date="2007">2007</year>
+          <volume>86</volume>
+          <fpage>423</fpage>
+          <lpage>430</lpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-39">
+        <label>Lattin et al. (2011)</label>
+        <element-citation publication-type="book">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Lattin</surname>
+              <given-names>C</given-names>
+            </name>
+            <name>
+              <surname>Waldron-Francis</surname>
+              <given-names>K</given-names>
+            </name>
+            <name>
+              <surname>Richardson</surname>
+              <given-names>J</given-names>
+            </name>
+            <name>
+              <surname>Breuner</surname>
+              <given-names>C</given-names>
+            </name>
+            <name>
+              <surname>Romero</surname>
+              <given-names>L</given-names>
+            </name>
+          </person-group>
+          <article-title>Quantification of glucocorticoid and mineralocorticoid receptors in House Sparrow brain and peripheral tissues</article-title>
+          <source>Society for integrative and comparative biology annual meeting 2011</source>
+          <year iso-8601-date="2011">2011</year>
+          <fpage>P3.42</fpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-40">
+        <label>Li et al. (2008)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Li</surname>
+              <given-names>D</given-names>
+            </name>
+            <name>
+              <surname>Wang</surname>
+              <given-names>G</given-names>
+            </name>
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>J</given-names>
+            </name>
+            <name>
+              <surname>Zhang</surname>
+              <given-names>Z</given-names>
+            </name>
+            <name>
+              <surname>Ding</surname>
+              <given-names>C</given-names>
+            </name>
+            <name>
+              <surname>Lei</surname>
+              <given-names>F</given-names>
+            </name>
+          </person-group>
+          <article-title>Seasonal changes in adrenocortical responses to acute stress in Eurasian tree sparrow (<italic>Passer montanus</italic>) on the Tibetan Plateau: comparison with house sparrow (<italic>P. domesticus</italic>) in North America and with the migratory <italic>P. domesticus</italic> in Qinghai Province</article-title>
+          <source>General and Comparative Endocrinology</source>
+          <year iso-8601-date="2008">2008</year>
+          <volume>158</volume>
+          <fpage>47</fpage>
+          <lpage>53</lpage>
+          <pub-id pub-id-type="doi">10.1016/j.ygcen.2008.06.002</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-41">
+        <label>Lynn, Breuner &amp; Wingfield (2003)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Lynn</surname>
+              <given-names>S</given-names>
+            </name>
+            <name>
+              <surname>Breuner</surname>
+              <given-names>C</given-names>
+            </name>
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>J</given-names>
+            </name>
+          </person-group>
+          <article-title>Short-term fasting affects locomotor activity, corticosterone, and corticosterone binding globulin in a migratory songbird</article-title>
+          <source>Hormones and Behavior</source>
+          <year iso-8601-date="2003">2003</year>
+          <volume>43</volume>
+          <fpage>150</fpage>
+          <lpage>157</lpage>
+          <pub-id pub-id-type="doi">10.1016/S0018-506X(02)00023-5</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-42">
+        <label>Maywood et al. (1993)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Maywood</surname>
+              <given-names>E</given-names>
+            </name>
+            <name>
+              <surname>Hastings</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Max</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Ampleford</surname>
+              <given-names>E</given-names>
+            </name>
+            <name>
+              <surname>Menaker</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Loudon</surname>
+              <given-names>A</given-names>
+            </name>
+          </person-group>
+          <article-title>Circadian and daily rhythms of melatonin in the blood and pineal-gland of free-running and entrained Syrian hamsters</article-title>
+          <source>Journal of Endocrinology</source>
+          <year iso-8601-date="1993">1993</year>
+          <volume>136</volume>
+          <fpage>65</fpage>
+          <lpage>73</lpage>
+          <pub-id pub-id-type="doi">10.1677/joe.0.1360065</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-43">
+        <label>McGuire &amp; Bentley (2010a)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>McGuire</surname>
+              <given-names>N</given-names>
+            </name>
+            <name>
+              <surname>Bentley</surname>
+              <given-names>G</given-names>
+            </name>
+          </person-group>
+          <article-title>A functional neuropeptide system in vertebrate gonads: gonadotropin-inhibitory hormone and its receptor in testes of field-caught house sparrow (<italic>Passer domesticus</italic>)</article-title>
+          <source>General and Comparative Endocrinology</source>
+          <year iso-8601-date="2010">2010a</year>
+          <volume>166</volume>
+          <fpage>565</fpage>
+          <lpage>572</lpage>
+          <pub-id pub-id-type="doi">10.1016/j.ygcen.2010.01.010</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-44">
+        <label>McGuire &amp; Bentley (2010b)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>McGuire</surname>
+              <given-names>N</given-names>
+            </name>
+            <name>
+              <surname>Bentley</surname>
+              <given-names>G</given-names>
+            </name>
+          </person-group>
+          <article-title>Neuropeptides in the gonads: from evolution to pharmacology</article-title>
+          <source>Frontiers in Integrative Pharmacology</source>
+          <year iso-8601-date="2010">2010b</year>
+          <volume>1</volume>
+          <fpage>114</fpage>
+          <pub-id pub-id-type="doi">10.3389/fphar.2010.00114</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-45">
+        <label>McGuire, Kangas &amp; Bentley (2011)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>McGuire</surname>
+              <given-names>NL</given-names>
+            </name>
+            <name>
+              <surname>Kangas</surname>
+              <given-names>K</given-names>
+            </name>
+            <name>
+              <surname>Bentley</surname>
+              <given-names>GE</given-names>
+            </name>
+          </person-group>
+          <article-title>Effects of melatonin on peripheral reproductive function: regulation of testicular GnIH and testosterone</article-title>
+          <source>Endocrinology</source>
+          <year iso-8601-date="2011">2011</year>
+          <volume>152</volume>
+          <fpage>3461</fpage>
+          <lpage>3470</lpage>
+          <pub-id pub-id-type="doi">10.1210/en.2011-1053</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-46">
+        <label>Morton (2002)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Morton</surname>
+              <given-names>M</given-names>
+            </name>
+          </person-group>
+          <article-title>The mountain white-crowned sparrow: migration and reproduction at high altitude</article-title>
+          <source>Studies in Avian Biology</source>
+          <year iso-8601-date="2002">2002</year>
+          <volume>24</volume>
+          <fpage>1</fpage>
+          <lpage>236</lpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-47">
+        <label>Murayama et al. (1997)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Murayama</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>Kawashima</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Takahashi</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>Yasuoka</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>Kuwayama</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>Tanaka</surname>
+              <given-names>K</given-names>
+            </name>
+          </person-group>
+          <article-title>Direct action of melatonin on hen ovarian granulosa cells to lower responsiveness to luteinizing hormone</article-title>
+          <source>Proceedings of the Society for Experimental Biology and Medicine</source>
+          <year iso-8601-date="1997">1997</year>
+          <volume>215</volume>
+          <fpage>386</fpage>
+          <lpage>392</lpage>
+          <pub-id pub-id-type="doi">10.3181/00379727-215-44148</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-48">
+        <label>Nicholls et al. (1987)</label>
+        <element-citation publication-type="book">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Nicholls</surname>
+              <given-names>TJ</given-names>
+            </name>
+            <name>
+              <surname>Goldsmith</surname>
+              <given-names>AR</given-names>
+            </name>
+            <name>
+              <surname>Follett</surname>
+              <given-names>BK</given-names>
+            </name>
+            <name>
+              <surname>Dawson</surname>
+              <given-names>A</given-names>
+            </name>
+          </person-group>
+          <person-group person-group-type="editor">
+            <name>
+              <surname>Pevet</surname>
+              <given-names>P</given-names>
+            </name>
+          </person-group>
+          <article-title>The photoperiodic responses of European starlings (<italic>Sturnus vulgaris</italic>)</article-title>
+          <source>Comparative physiology of environmental adaptations: adaptations to climatic changes v. 3</source>
+          <year iso-8601-date="1987">1987</year>
+          <publisher-loc>Basel</publisher-loc>
+          <publisher-name>S Karger AG</publisher-name>
+          <fpage>23</fpage>
+          <lpage>29</lpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-49">
+        <label>Nicholls, Goldsmith &amp; Dawson (1988)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Nicholls</surname>
+              <given-names>TJ</given-names>
+            </name>
+            <name>
+              <surname>Goldsmith</surname>
+              <given-names>AR</given-names>
+            </name>
+            <name>
+              <surname>Dawson</surname>
+              <given-names>A</given-names>
+            </name>
+          </person-group>
+          <article-title>Photorefractoriness in birds and comparison with mammals</article-title>
+          <source>Physiological Research</source>
+          <year iso-8601-date="1988">1988</year>
+          <volume>68</volume>
+          <fpage>133</fpage>
+          <lpage>176</lpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-50">
+        <label>Niedziela, Lerchl &amp; Nieschlag (1995)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Niedziela</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Lerchl</surname>
+              <given-names>A</given-names>
+            </name>
+            <name>
+              <surname>Nieschlag</surname>
+              <given-names>E</given-names>
+            </name>
+          </person-group>
+          <article-title>Direct effects of the pineal hormone melatonin on testosterone synthesis of Leydig cells in Djungarian hamsters (<italic>Phodopus sungorus</italic>) in vitro</article-title>
+          <source>Neuroscience Letters</source>
+          <year iso-8601-date="1995">1995</year>
+          <volume>201</volume>
+          <fpage>247</fpage>
+          <lpage>250</lpage>
+          <pub-id pub-id-type="doi">10.1016/0304-3940(95)12183-8</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-51">
+        <label>Rich &amp; Romero (2005)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Rich</surname>
+              <given-names>E</given-names>
+            </name>
+            <name>
+              <surname>Romero</surname>
+              <given-names>L</given-names>
+            </name>
+          </person-group>
+          <article-title>Exposure to chronic stress downregulates corticosterone responses to acute stressors</article-title>
+          <source>American Journal of Physiology: Regulatory Integrative and Comparative Physiology</source>
+          <year iso-8601-date="2005">2005</year>
+          <volume>288</volume>
+          <fpage>R1628</fpage>
+          <lpage>R1636</lpage>
+          <pub-id pub-id-type="doi">10.1152/ajpregu.00484.2004</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-52">
+        <label>Rivest, Plotsky &amp; Rivier (1993)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Rivest</surname>
+              <given-names>S</given-names>
+            </name>
+            <name>
+              <surname>Plotsky</surname>
+              <given-names>P</given-names>
+            </name>
+            <name>
+              <surname>Rivier</surname>
+              <given-names>C</given-names>
+            </name>
+          </person-group>
+          <article-title>CRF alters the infundibular LHRH secretory system from the medial preoptic area of female rats: possible involvement of opiod receptors</article-title>
+          <source>Neuroendocrinology</source>
+          <year iso-8601-date="1993">1993</year>
+          <volume>57</volume>
+          <fpage>236</fpage>
+          <lpage>246</lpage>
+          <pub-id pub-id-type="doi">10.1159/000126365</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-53">
+        <label>Rollag, O’Callaghan &amp; Niswender (1978)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Rollag</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>O’Callaghan</surname>
+              <given-names>P</given-names>
+            </name>
+            <name>
+              <surname>Niswender</surname>
+              <given-names>G</given-names>
+            </name>
+          </person-group>
+          <article-title>Serum melatonin concentrations during different stages of the annual reproductive cycle in ewes</article-title>
+          <source>Reproductive and Biological Endocrinology</source>
+          <year iso-8601-date="1978">1978</year>
+          <volume>18</volume>
+          <fpage>279</fpage>
+          <lpage>285</lpage>
+          <pub-id pub-id-type="doi">10.1095/biolreprod18.2.279</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-54">
+        <label>Romero (2002)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Romero</surname>
+              <given-names>L</given-names>
+            </name>
+          </person-group>
+          <article-title>Seasonal changes in plasma glucocorticoid concentrations in free-living vertebrates</article-title>
+          <source>General and Comparative Endocrinology</source>
+          <year iso-8601-date="2002">2002</year>
+          <volume>128</volume>
+          <fpage>1</fpage>
+          <lpage>24</lpage>
+          <pub-id pub-id-type="doi">10.1016/S0016-6480(02)00064-3</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-55">
+        <label>Romero &amp; Remage-Healey (2000)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Romero</surname>
+              <given-names>L</given-names>
+            </name>
+            <name>
+              <surname>Remage-Healey</surname>
+              <given-names>L</given-names>
+            </name>
+          </person-group>
+          <article-title>Daily and seasonal variation in response to stress in captive starlings (<italic>Sturnus vulgaris</italic>): corticosterone</article-title>
+          <source>General and Comparative Endocrinology</source>
+          <year iso-8601-date="2000">2000</year>
+          <volume>119</volume>
+          <fpage>52</fpage>
+          <lpage>59</lpage>
+          <pub-id pub-id-type="doi">10.1006/gcen.2000.7491</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-56">
+        <label>Schneider (2004)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Schneider</surname>
+              <given-names>JE</given-names>
+            </name>
+          </person-group>
+          <article-title>Energy balance and reproduction</article-title>
+          <source>Physiology and Behavior</source>
+          <year iso-8601-date="2004">2004</year>
+          <volume>81</volume>
+          <fpage>289</fpage>
+          <lpage>317</lpage>
+          <pub-id pub-id-type="doi">10.1016/j.physbeh.2004.02.007</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-57">
+        <label>Spencer &amp; Christensen (1999)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Spencer</surname>
+              <given-names>W</given-names>
+            </name>
+            <name>
+              <surname>Christensen</surname>
+              <given-names>M</given-names>
+            </name>
+          </person-group>
+          <article-title>Multiplex relative RT-PCR method for verification of differential gene expression</article-title>
+          <source>Biotechniques</source>
+          <year iso-8601-date="1999">1999</year>
+          <volume>27</volume>
+          <fpage>1048</fpage>
+          <lpage>1052</lpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-58">
+        <label>Szymanski et al. (2007)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Szymanski</surname>
+              <given-names>LA</given-names>
+            </name>
+            <name>
+              <surname>Schneider</surname>
+              <given-names>JE</given-names>
+            </name>
+            <name>
+              <surname>Friedman</surname>
+              <given-names>MI</given-names>
+            </name>
+            <name>
+              <surname>Ji</surname>
+              <given-names>H</given-names>
+            </name>
+            <name>
+              <surname>Kurose</surname>
+              <given-names>Y</given-names>
+            </name>
+            <name>
+              <surname>Blache</surname>
+              <given-names>D</given-names>
+            </name>
+            <name>
+              <surname>Rao</surname>
+              <given-names>A</given-names>
+            </name>
+            <name>
+              <surname>Dunshea</surname>
+              <given-names>FR</given-names>
+            </name>
+            <name>
+              <surname>Clarke</surname>
+              <given-names>IJ</given-names>
+            </name>
+          </person-group>
+          <article-title>Changes in insulin, glucose and ketone bodies, but not leptin or body fat content precede restoration of luteinising hormone secretion in ewes</article-title>
+          <source>Journal of Neuroendocrinology</source>
+          <year iso-8601-date="2007">2007</year>
+          <volume>19</volume>
+          <fpage>449</fpage>
+          <lpage>460</lpage>
+          <pub-id pub-id-type="doi">10.1111/j.1365-2826.2007.01551.x</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-59">
+        <label>Tsutsui et al. (2000)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Tsutsui</surname>
+              <given-names>K</given-names>
+            </name>
+            <name>
+              <surname>Saigoh</surname>
+              <given-names>E</given-names>
+            </name>
+            <name>
+              <surname>Ukena</surname>
+              <given-names>K</given-names>
+            </name>
+            <name>
+              <surname>Teranishi</surname>
+              <given-names>H</given-names>
+            </name>
+            <name>
+              <surname>Fujisawa</surname>
+              <given-names>Y</given-names>
+            </name>
+            <name>
+              <surname>Kikuchi</surname>
+              <given-names>M</given-names>
+            </name>
+            <name>
+              <surname>Ishii</surname>
+              <given-names>S</given-names>
+            </name>
+            <name>
+              <surname>Sharp</surname>
+              <given-names>P</given-names>
+            </name>
+          </person-group>
+          <article-title>A novel avian hypothalamic peptide inhibiting gonadotropin release</article-title>
+          <source>Biochemical and Biophysical Research Communications</source>
+          <year iso-8601-date="2000">2000</year>
+          <volume>275</volume>
+          <fpage>661</fpage>
+          <lpage>667</lpage>
+          <pub-id pub-id-type="doi">10.1006/bbrc.2000.3350</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-60">
+        <label>Ubuka et al. (2005)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Ubuka</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>Bentley</surname>
+              <given-names>G</given-names>
+            </name>
+            <name>
+              <surname>Ukena</surname>
+              <given-names>K</given-names>
+            </name>
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>J</given-names>
+            </name>
+            <name>
+              <surname>Tsutsui</surname>
+              <given-names>K</given-names>
+            </name>
+          </person-group>
+          <article-title>Melatonin induces the expression of gonadotropin-inhibitory hormone in the avian brain</article-title>
+          <source>Proceedings of the National Academy of Sciences</source>
+          <year iso-8601-date="2005">2005</year>
+          <volume>102</volume>
+          <fpage>3052</fpage>
+          <lpage>3057</lpage>
+          <pub-id pub-id-type="doi">10.1073/pnas.0403840102</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-61">
+        <label>Ubuka et al. (2008)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Ubuka</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>Kim</surname>
+              <given-names>S</given-names>
+            </name>
+            <name>
+              <surname>Huang</surname>
+              <given-names>Y</given-names>
+            </name>
+            <name>
+              <surname>Reid</surname>
+              <given-names>J</given-names>
+            </name>
+            <name>
+              <surname>Jiang</surname>
+              <given-names>J</given-names>
+            </name>
+            <name>
+              <surname>Osugi</surname>
+              <given-names>T</given-names>
+            </name>
+            <name>
+              <surname>Chowdhury</surname>
+              <given-names>V</given-names>
+            </name>
+            <name>
+              <surname>Tsutsui</surname>
+              <given-names>K</given-names>
+            </name>
+            <name>
+              <surname>Bentley</surname>
+              <given-names>G</given-names>
+            </name>
+          </person-group>
+          <article-title>Gonadotropin-inhibitory hormone neurons interact directly with gonadotropin-releasing hormone-I and -II neurons in European starling brain</article-title>
+          <source>Endocrinology</source>
+          <year iso-8601-date="2008">2008</year>
+          <volume>149</volume>
+          <fpage>268</fpage>
+          <lpage>278</lpage>
+          <pub-id pub-id-type="doi">10.1210/en.2007-0983</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-62">
+        <label>Vezina &amp; Salvante (2010)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Vezina</surname>
+              <given-names>F</given-names>
+            </name>
+            <name>
+              <surname>Salvante</surname>
+              <given-names>K</given-names>
+            </name>
+          </person-group>
+          <article-title>Behavioral and physiological flexibility are used by birds to manage energy and support investment in the early stages of reproduction</article-title>
+          <source>Current Zoology</source>
+          <year iso-8601-date="2010">2010</year>
+          <volume>56</volume>
+          <fpage>767</fpage>
+          <lpage>792</lpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-63">
+        <label>Wick et al. (1957)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Wick</surname>
+              <given-names>A</given-names>
+            </name>
+            <name>
+              <surname>Dury</surname>
+              <given-names>D</given-names>
+            </name>
+            <name>
+              <surname>Nakada</surname>
+              <given-names>H</given-names>
+            </name>
+            <name>
+              <surname>Wolfe</surname>
+              <given-names>J</given-names>
+            </name>
+          </person-group>
+          <article-title>Localization of the primary metabolic block produced by 2-deoxyglucose</article-title>
+          <source>Journal of Biological Chemistry</source>
+          <year iso-8601-date="1957">1957</year>
+          <volume>224</volume>
+          <fpage>963</fpage>
+          <lpage>969</lpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-64">
+        <label>Wingfield &amp; Ramenofsky (1997)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>J</given-names>
+            </name>
+            <name>
+              <surname>Ramenofsky</surname>
+              <given-names>M</given-names>
+            </name>
+          </person-group>
+          <article-title>Corticosterone and facultative dispersal in responses to unpredictable events</article-title>
+          <source>Ardea</source>
+          <year iso-8601-date="1997">1997</year>
+          <volume>85</volume>
+          <fpage>155</fpage>
+          <lpage>166</lpage>
+        </element-citation>
+      </ref>
+      <ref id="ref-65">
+        <label>Wingfield &amp; Sapolsky (2003)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>J</given-names>
+            </name>
+            <name>
+              <surname>Sapolsky</surname>
+              <given-names>R</given-names>
+            </name>
+          </person-group>
+          <article-title>Reproduction and resistance to stress: when and how</article-title>
+          <source>Journal of Neuroendocrinology</source>
+          <year iso-8601-date="2003">2003</year>
+          <volume>15</volume>
+          <fpage>711</fpage>
+          <lpage>724</lpage>
+          <pub-id pub-id-type="doi">10.1046/j.1365-2826.2003.01033.x</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-66">
+        <label>Wingfield, Vieck &amp; Moore (1992)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>J</given-names>
+            </name>
+            <name>
+              <surname>Vieck</surname>
+              <given-names>C</given-names>
+            </name>
+            <name>
+              <surname>Moore</surname>
+              <given-names>I</given-names>
+            </name>
+          </person-group>
+          <article-title>Seasonal changes of the adrenocortical response to stress in birds of the Sonoran Desert</article-title>
+          <source>Journal of Experimental Zoology</source>
+          <year iso-8601-date="1992">1992</year>
+          <volume>264</volume>
+          <fpage>419</fpage>
+          <lpage>428</lpage>
+          <pub-id pub-id-type="doi">10.1002/jez.1402640407</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-67">
+        <label>Wingfield (1984)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>JC</given-names>
+            </name>
+          </person-group>
+          <article-title>Influence of weather on reproduction</article-title>
+          <source>Journal of Experimental Zoology</source>
+          <year iso-8601-date="1984">1984</year>
+          <volume>232</volume>
+          <fpage>589</fpage>
+          <lpage>594</lpage>
+          <pub-id pub-id-type="doi">10.1002/jez.1402320327</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-68">
+        <label>Wingfield (1985a)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>JC</given-names>
+            </name>
+          </person-group>
+          <article-title>Influences of weather on reproductive function in male song sparrows. <italic>Melospiza melodia</italic></article-title>
+          <source>Journal of Zoology</source>
+          <year iso-8601-date="1985">1985a</year>
+          <volume>205</volume>
+          <fpage>525</fpage>
+          <lpage>544</lpage>
+          <pub-id pub-id-type="doi">10.1111/j.1469-7998.1985.tb03542.x</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-69">
+        <label>Wingfield (1985b)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>JC</given-names>
+            </name>
+          </person-group>
+          <article-title>Influences of weather on reproductive function in female song sparrows. <italic>Melospiza melodia</italic></article-title>
+          <source>Journal of Zoology</source>
+          <year iso-8601-date="1985">1985b</year>
+          <volume>205</volume>
+          <fpage>545</fpage>
+          <lpage>558</lpage>
+          <pub-id pub-id-type="doi">10.1111/j.1469-7998.1985.tb03543.x</pub-id>
+        </element-citation>
+      </ref>
+      <ref id="ref-70">
+        <label>Wingfield, Moore &amp; Farner (1983)</label>
+        <element-citation publication-type="journal">
+          <person-group person-group-type="author">
+            <name>
+              <surname>Wingfield</surname>
+              <given-names>JC</given-names>
+            </name>
+            <name>
+              <surname>Moore</surname>
+              <given-names>MC</given-names>
+            </name>
+            <name>
+              <surname>Farner</surname>
+              <given-names>DS</given-names>
+            </name>
+          </person-group>
+          <article-title>Endocrine responses to inclement weather in naturally breeding populations of white-crowned sparrows. (<italic>Zonotrichia leucophrys pugetensis</italic>)</article-title>
+          <source>Auk</source>
+          <year iso-8601-date="1983">1983</year>
+          <volume>100</volume>
+          <fpage>56</fpage>
+          <lpage>62</lpage>
+        </element-citation>
+      </ref>
+    </ref-list>
+  </back>
+</article>