intermine-bio 0.98.1
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- data/Gemfile +4 -0
- data/LICENCE +165 -0
- data/README.rdoc +50 -0
- data/Rakefile +62 -0
- data/contact_header.rdoc +10 -0
- data/lib/intermine/bio.rb +221 -0
- data/test/data/test.bed +8 -0
- data/test/data/test.fasta +84 -0
- data/test/data/test.gff3 +5 -0
- data/test/test_helper.rb +67 -0
- data/test/unit_tests.rb +112 -0
- metadata +146 -0
data/Gemfile
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data/LICENCE
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GNU LESSER GENERAL PUBLIC LICENSE
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Version 3, 29 June 2007
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Copyright (C) 2007 Free Software Foundation, Inc. <http://fsf.org/>
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Everyone is permitted to copy and distribute verbatim copies
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of this license document, but changing it is not allowed.
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This version of the GNU Lesser General Public License incorporates
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the terms and conditions of version 3 of the GNU General Public
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License, supplemented by the additional permissions listed below.
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0. Additional Definitions.
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As used herein, "this License" refers to version 3 of the GNU Lesser
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General Public License, and the "GNU GPL" refers to version 3 of the GNU
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General Public License.
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An "Application" is any work that makes use of an interface provided
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data/README.rdoc
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= Biological Extensions to the InterMine Webservice Client Library
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This library is a set of extensions to the InterMine Webservices client,
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providing access for data in biological formats. It directly extends the
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InterMine classes, providing extra methods to the Query class.
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== Example
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Get all sequences for proteins on "h", "r", "eve", "bib" and "zen":
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require "rubygems"
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require "intermine/service"
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require "intermine/bio"
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s = Service.new("www.flymine.org/query")
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puts s.query("Gene").select("proteins").where(:symbol => %w{h r eve bib zen}).fasta
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Process the locations of these genes one at a time:
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s.query.select("Gene").where(:symbol => %w{h r eve bib zen}).bed do |line|
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process line
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end
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== Who is this for?
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InterMine data warehouses are typically constructed to hold
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Biological data, and as this library facilitates programmatic
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access to these data, this install is primarily aimed at
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bioinformaticians. In particular, users of the following services
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may find it especially useful:
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* FlyMine (http://www.flymine.org/query)
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* YeastMine (http://yeastmine.yeastgenome.org/yeastmine)
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* RatMine (http://ratmine.mcw.edu/ratmine)
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* modMine (http://intermine.modencode.org/release-23)
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* metabolicMine (http://www.metabolicmine.org/beta)
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These extensions are aimed at bioinformaticians looking to integrate
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these sources of data into other workflows.
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For details on constructing queries, see the intermine documentation.
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== Support
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Support is available on our development mailing list: dev@intermine.org
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== License
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This code is Open Source under the LGPL. Source code for all InterMine code
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can be checked out from svn://subversion.flymine.org/flymine
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data/Rakefile
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require 'rubygems'
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require 'rubygems/specification' unless defined?(Gem::Specification)
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require 'rake/testtask'
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require 'rake/gempackagetask'
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gem 'rdoc', '=2.1.0'
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require 'rdoc/rdoc'
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require 'rake/rdoctask'
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gem 'darkfish-rdoc'
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require 'darkfish-rdoc'
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def gemspec
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@gemspec ||= begin
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Gem::Specification.load(File.expand_path('intermine-bio.gemspec'))
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end
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end
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task :default => :test
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desc 'Start a console session'
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task :console do
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system 'irb -I lib -r rubygems -r intermine/service -r intermine/bio'
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end
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desc 'Displays the current version'
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task :version do
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puts "Current version: #{gemspec.version}"
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end
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desc 'Installs the gem locally'
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task :install => :package do
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sh "gem install pkg/#{gemspec.name}-#{gemspec.version}"
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end
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desc 'Release the gem'
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task :release => :package do
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sh "gem push pkg/#{gemspec.name}-#{gemspec.version}.gem"
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end
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Rake::GemPackageTask.new(gemspec) do |pkg|
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pkg.need_zip = true
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pkg.need_tar = true
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end
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Rake::TestTask.new do |t|
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t.libs << "test"
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t.test_files = FileList['test/unit_tests.rb']
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t.verbose = true
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t.warning = true
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end
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Rake::RDocTask.new do |t|
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t.title = 'Bio Extensions to the InterMine Webservice Client'
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t.rdoc_files.include 'README.rdoc'
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t.rdoc_files.include 'lib/**/*rb'
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t.main = 'README.rdoc'
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t.options += ['-SHN', '-f', 'darkfish']
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end
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data/contact_header.rdoc
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#
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# [author] Alex Kalderimis dev@intermine.org
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# [homepage] http://www.intermine.org
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# [Licence] Copyright (C) 2002-2011 FlyMine
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#
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# This code may be freely distributed and modified under the
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# terms of the GNU Lesser General Public Licence. This should
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# be distributed with the code. See the LICENSE file for more
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# information or http://www.gnu.org/copyleft/lesser.html.
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#
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require "net/http"
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# == Biologically specific Extensions to the InterMine Data-Warehousing Webservices
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#
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# These modules provide interfaces to the biologically specific elements of the
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# InterMine webservices. At present this consists of the ability to return results
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# from queries in Biologically specific formats (GFF3, UCSC-BED, FASTA). The
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# methods for accessing these formats provide mechanisms for iterating over the contents
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# in logical chunks.
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#
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#:include:contact_header.rdoc
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#
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module InterMine
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# The Metadata for these extensions
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#
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#:include:contact_header.rdoc
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#
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module Bio
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# The library version
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VERSION = "0.98.1"
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# The library name
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NAME = "intermine-bio"
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# The project's homepage
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HOMEPAGE = "http://www.intermine.org"
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# The authors of this library
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AUTHORS = ["Alex Kalderimis"]
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# An email address to seek support at
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EMAIL = ["dev@intermine.org"]
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end
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# Extensions to the PathQuery module.
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#
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#:include:contact_header.rdoc
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#
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module PathQuery
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class BioError < RuntimeError
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end
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# Biologically specific Extensions to the Query class.
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#
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# These methods provide mechanisms for accessing results in Biologically
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# appropriate formats, being at present GFF3, FASTA, and UCSC-BED.
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#
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# The methods provided here can be used to both return the data as a string,
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# and to iterate over the data in logical chunks, approriate to the format in question.
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#
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#:include:contact_header.rdoc
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#
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class Query
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# Return the results from this query as GFF3
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#
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# If a block is given, each line of the GFF3 will be
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# yielded in turn, omitting any header lines, which are
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# returned at the end of the iteration, otherwise
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# the content of the GFF3 results will be returned as one string.
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#
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# header = query.gff3 do |line|
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# process line
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# end
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#
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# puts query.gff3
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#
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def gff3
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if block_given?
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header = ""
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results_reader.each_gff3 do |gff3|
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if gff3.start_with? "#"
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header << gff3
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else
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|
+
yield gff3
|
79
|
+
end
|
80
|
+
end
|
81
|
+
return header
|
82
|
+
else
|
83
|
+
buffer = ""
|
84
|
+
results_reader.each_gff3 do |gff3|
|
85
|
+
buffer.concat(gff3)
|
86
|
+
end
|
87
|
+
return buffer
|
88
|
+
end
|
89
|
+
end
|
90
|
+
|
91
|
+
# Return the results from this query as FASTA
|
92
|
+
#
|
93
|
+
# If a block is given, each FASTA record will be yielded
|
94
|
+
# in turn, and the query will be returned, otherwise
|
95
|
+
# the content of the FASTA results will be returned as one string.
|
96
|
+
#
|
97
|
+
# query.fasta do |record|
|
98
|
+
# process record
|
99
|
+
# end
|
100
|
+
#
|
101
|
+
# puts query.fasta
|
102
|
+
#
|
103
|
+
def fasta # :yields: record
|
104
|
+
if block_given?
|
105
|
+
buffer = nil
|
106
|
+
results_reader.each_fasta do |line|
|
107
|
+
if line.start_with? ">"
|
108
|
+
yield buffer unless buffer.nil?
|
109
|
+
buffer = line
|
110
|
+
else
|
111
|
+
raise BioError, "Incorrect fasta - no header line" if buffer.nil?
|
112
|
+
buffer << line
|
113
|
+
end
|
114
|
+
end
|
115
|
+
yield buffer unless buffer.nil?
|
116
|
+
return self
|
117
|
+
else
|
118
|
+
buffer = ""
|
119
|
+
results_reader.each_fasta do |line|
|
120
|
+
buffer.concat(line)
|
121
|
+
end
|
122
|
+
return buffer
|
123
|
+
end
|
124
|
+
end
|
125
|
+
|
126
|
+
# Return the results from this query as BED
|
127
|
+
#
|
128
|
+
# If a block is given, each line of the BED results will be
|
129
|
+
# yielded in turn, and the header will be returned, otherwise
|
130
|
+
# the content of the BED results will be returned as one string.
|
131
|
+
#
|
132
|
+
# If the optional parameter is set to false, then the "chr" prefix on
|
133
|
+
# the chromosome id will be omitted.
|
134
|
+
#
|
135
|
+
# header = query.bed do |line|
|
136
|
+
# process line
|
137
|
+
# end
|
138
|
+
#
|
139
|
+
# puts query.bed(false)
|
140
|
+
#
|
141
|
+
def bed(ucscCompatible=true)
|
142
|
+
if block_given?
|
143
|
+
header = ""
|
144
|
+
results_reader.each_bed(ucscCompatible) do |bed|
|
145
|
+
if bed =~ /^\s*(#|track)/
|
146
|
+
header << bed
|
147
|
+
else
|
148
|
+
yield bed
|
149
|
+
end
|
150
|
+
end
|
151
|
+
return header
|
152
|
+
else
|
153
|
+
buffer = ""
|
154
|
+
results_reader.each_bed(ucscCompatible) do |bed|
|
155
|
+
buffer.concat(bed)
|
156
|
+
end
|
157
|
+
return buffer
|
158
|
+
end
|
159
|
+
end
|
160
|
+
end
|
161
|
+
end
|
162
|
+
|
163
|
+
# Extensions to the Result processing code
|
164
|
+
#
|
165
|
+
#:include:contact_header.rdoc
|
166
|
+
#
|
167
|
+
module Results
|
168
|
+
|
169
|
+
# Extensions to the ResultsReader object
|
170
|
+
#
|
171
|
+
# These methods provide mechanisms for accessing the results in raw
|
172
|
+
# format, and iterating over them line by line in a memory efficient
|
173
|
+
# manner. They are in no way content aware.
|
174
|
+
#
|
175
|
+
#:include:contact_header.rdoc
|
176
|
+
#
|
177
|
+
class ResultsReader
|
178
|
+
|
179
|
+
# The path to use to get the resource paths for the query variants
|
180
|
+
RESOURCE_PATH = "/check/"
|
181
|
+
|
182
|
+
# Yield results as GFF3
|
183
|
+
def each_gff3
|
184
|
+
adjust_path(:gff3)
|
185
|
+
each_line(params("gff3")) do |line|
|
186
|
+
yield line
|
187
|
+
end
|
188
|
+
end
|
189
|
+
|
190
|
+
# Yield results as fasta
|
191
|
+
def each_fasta
|
192
|
+
adjust_path(:fasta)
|
193
|
+
each_line(params("fasta")) do |line|
|
194
|
+
yield line
|
195
|
+
end
|
196
|
+
end
|
197
|
+
|
198
|
+
# Yield results as UCSC-BED
|
199
|
+
def each_bed(ucscCompatible=true)
|
200
|
+
adjust_path(:bed)
|
201
|
+
p = params("bed")
|
202
|
+
p["ucscCompatible"] = "no" unless ucscCompatible
|
203
|
+
each_line(p) do |line|
|
204
|
+
yield line
|
205
|
+
end
|
206
|
+
end
|
207
|
+
|
208
|
+
# Adjust the path of this query to suit the currently selected format.
|
209
|
+
def adjust_path(variant)
|
210
|
+
@resources ||= {}
|
211
|
+
root = @query.service.root
|
212
|
+
uri = URI.parse(root + RESOURCE_PATH + "query." + variant.to_s)
|
213
|
+
@resources[variant] = Net::HTTP.get(uri.host, uri.path)
|
214
|
+
path = @resources[variant]
|
215
|
+
@uri = URI.parse(root + path)
|
216
|
+
end
|
217
|
+
|
218
|
+
end
|
219
|
+
end
|
220
|
+
end
|
221
|
+
|
data/test/data/test.bed
ADDED
@@ -0,0 +1,8 @@
|
|
1
|
+
# UCSC BED format
|
2
|
+
# Source: FlyMine
|
3
|
+
# Genome Build: dm3
|
4
|
+
track name=FlyMine_newtpreview_Custom_Track description="FlyMine newtpreview Custom Track" useScore=0
|
5
|
+
chr2R 5866745 5868284 eve 0 +
|
6
|
+
chr2R 5866745 5867058 eve:1 0 +
|
7
|
+
chr2R 5866745 5868284 eve-RA 0 +
|
8
|
+
chr2R 5867129 5868284 eve:2 0 +
|
@@ -0,0 +1,84 @@
|
|
1
|
+
>ZEN1_DROME 362011840
|
2
|
+
MSSVMHYYPVHQAKVGSYSADPSEVKYSDLIYGHHHDVNPIGLPPNYNQMNSNPTTLNDH
|
3
|
+
CSPQHVHQQHVSSDENLPSQPNHDSQRVKLKRSRTAFTSVQLVELENEFKSNMYLYRTRR
|
4
|
+
IEIAQRLSLCERQVKIWFQNRRMKFKKDIQGHREPKSNAKLAQPQAEQSAHRGIVKRLMS
|
5
|
+
YSQDPREGTAAAEKRPMMAVAPVNPKPDYQASQKMKTEASTNNGMCSSADLSEILEHLAQ
|
6
|
+
TTAAPQVSTATSSTGTSTNSASSSSSGHYSYNVDLVLQSIKQDLEAAAQAWSKSKSAPIL
|
7
|
+
ATQSWHPSSQSQVPTSVHAAPSMNLSWGEPAAKSRKLSVNHMNPCVTSYNYPN
|
8
|
+
>PYR1_DROME 362022982
|
9
|
+
MASTDCYLALEDGTVLPGYSFGYVPSENESKVGFGGEVVFQTGMVGYTEALTDRSYSGQI
|
10
|
+
LVLTYPLIGNYGVPAPDEDEHGLPLHFEWMKGVVQATALVVGEVAEEAFHWRKWKTLPDW
|
11
|
+
LKQHKVPGIQDIDTRALTKKLREQGSMLGKIVYEKPPVEGLPKSSFVDPNVRNLAKECSV
|
12
|
+
KERQVYGNPNGKGPRIAILDCGLKLNQLRCLLQRGASVTLLPWSARLEDEQFDALFLSNG
|
13
|
+
PGNPESCDQIVQQVRKVIEEGQKPVFGICLGHQLLAKAIGCSTYKMKYGNRGHNLPCLHR
|
14
|
+
ATGRCLMTSQNHGYAVDLEQLPDGWSELFVNANDGTNEGIVHASKPYFSVQFHPEHHAGP
|
15
|
+
QDTEFLFDVFMESIQQKDLTIPQLIEQRLRPTTPAIDSAPVMPRKVLILGSGGLSIGQAG
|
16
|
+
EFDYSGSQAIKAMRESNIQTVLINPNIATVQTSKGMADKCYFLPLTPHYVEQVIKSERPN
|
17
|
+
GVLLTFGGQTALNCGVQLERAGVFSKYNVRILGTPIQSIIETEDRKLFAERVNEIGEQVA
|
18
|
+
PSEAVYSVAQALDAASRLGYPVMARAAFSLGGLGSGFANNEEELQSLAQQALAHSSQLIV
|
19
|
+
DKSLKGWKEVEYEVVRDAYNNCITVCNMENFDPLGIHTGESIVVAPSQTLSDREYQMLRS
|
20
|
+
TALKVIRHFGVVGECNIQYALCPHSEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLA
|
21
|
+
LGLPLPDIKNSVTGNTTACFEPSLDYCVVKIPRWDLAKFVRVSKHIGSSMKSVGEVMAIG
|
22
|
+
RNFEEAFQKALRMVDSDVLGFDPDVVPLNKEQLAEQLSEPTDRRPFVIAAALQLGMSLRE
|
23
|
+
LHQLTNIDYWFLEKLERIILLQSLLTRNGSRTDAALLLKAKRFGFSDKQIAKYIKSTELA
|
24
|
+
VRHQRQEFGIRPHVKQIDTVAGEWPASTNYLYHTYNGSEHDVDFPGGHTIVVGSGVYRIG
|
25
|
+
SSVEFDWCAVGCLRELRKLQRPTIMINYNPETVSTDYDMCDRLYFEEISFEVVMDIYEME
|
26
|
+
NSEGIILSMGGQLPNNIAMDLHRQQAKVLGTSPESIDCAENRFKFSRMLDRKGILQPRWK
|
27
|
+
ELTNLQSAIEFCEEVGYPCLVRPSYVLSGAAMNVAYSNQDLETYLNAASEVSREHPVVIS
|
28
|
+
KFLTEAKEIDVDAVASDGRILCMAVSEHVENAGVHSGDATLVTPPQDLNAETLEAIKRIT
|
29
|
+
CDLASVLDVTGPFNMQLIAKNNELKVIECNVRVSRSFPFVSKTLDHDFVATATRAIVGLD
|
30
|
+
VEPLDVLHGVGKVGVKVPQFSFSRLAGADVQLGVEMASTGEVACFGDNRYEAYLKAMMST
|
31
|
+
GFQIPKNAVLLSIGSFKHKMELLPSIRDLAKMGYKLYASMGTGDFYAEHGVNVESVQWTF
|
32
|
+
DKTTPDDINGELRHLAEFLANKQFDLVINLPMSGGGARRVSSFMTHGYRTRRLAVDYSIP
|
33
|
+
LVTDVKCTKLLVESMRMNGGKPPMKTHTDCMTSRRIVKLPGFIDVHVHLREPGATHKEDF
|
34
|
+
ASGTAAALAGGVTLVCAMPNTNPSIVDRETFTQFQELAKAGARCDYALYVGASDDNWAQV
|
35
|
+
NELASHACGLKMYLNDTFGTLKLSDMTSWQRHLSHWPKRSPIVCHAERQSTAAVIMLAHL
|
36
|
+
LDRSVHICHVARKEEIQLIRSAKEKGVKVTCEVCPHHLFLSTKDVERLGHGMSEVRPLLC
|
37
|
+
SPEDQEALWENIDYIDVFATDHAPHTLAEKRSERPPPGFPGVETILPLLLQAVHEGRLTM
|
38
|
+
EDIKRKFHRNPKIIFNLPDQAQTYVEVDLDEEWTITGNEMKSKSGWTPFEGTKVKGRVHR
|
39
|
+
VVLRGEVAFVDGQVLVQPGFGQNVRPKQSPLASEASQDLLPSDNDANDTFTRLLTSEGPG
|
40
|
+
GGVHGISTKVHFVDGANFLRPNSPSPRIRLDSASNTTLREYLQRTTNSNPVAHSLMGKHI
|
41
|
+
LAVDMFNKDHLNDIFNLAQLLKLRGTKDRPVDELLPGKIMASVFYEVSTRTQCSFAAAML
|
42
|
+
RLGGRVISMDNITSSVKKGESLEDSIKVVSSYADVVVLRHPSPGAVARAATFSRKPLINA
|
43
|
+
GDGVGEHPTQALLDIFTIREEFGTVNGLTITMVGDLKNGRTVHSLARLLTLYNVNLQYVA
|
44
|
+
PNSLQMPDEVVQFVHQRGVKQLFARDLKNVLPDTDVLYMTRIQRERFDNVEDYEKCCGHL
|
45
|
+
VLTPEHMMRAKKRSIVLHPLPRLNEISREIDSDPRAAYFRQAEYGMYIRMALLAMVVGGR
|
46
|
+
NTAL
|
47
|
+
>FBpp0088674 362022983
|
48
|
+
MASTDCYLALEDGTVLPGYSFGYVPSENESKVGFGGEVVFQTGMVGYTEALTDRSYSGQI
|
49
|
+
LVLTYPLIGNYGVPAPDEDEHGLPLHFEWMKGVVQATALVVGEVAEEAFHWRKWKTLPDW
|
50
|
+
LKQHKVPGIQDIDTRALTKKLREQGSMLGKIVYEKPPVEGLPKSSFVDPNVRNLAKECSV
|
51
|
+
KERQVYGNPNGKGPRIAILDCGLKLNQLRCLLQRGASVTLLPWSARLEDEQFDALFLSNG
|
52
|
+
PGNPESCDQIVQQVRKVIEEGQKPVFGICLGHQLLAKAIGCSTYKMKYGNRGHNLPCLHR
|
53
|
+
ATGRCLMTSQNHGYAVDLEQLPDGWSELFVNANDGTNEGIVHASKPYFSVQFHPEHHAGP
|
54
|
+
QDTEFLFDVFMESIQQKDLTIPQLIEQRLRPTTPAIDSAPVMPRKVLILGSGGLSIGQAG
|
55
|
+
EFDYSGSQAIKAMRESNIQTVLINPNIATVQTSKGMADKCYFLPLTPHYVEQVIKSERPN
|
56
|
+
GVLLTFGGQTALNCGVQLERAGVFSKYNVRILGTPIQSIIETEDRKLFAERVNEIGEQVA
|
57
|
+
PSEAVYSVAQALDAASRLGYPVMARAAFSLGGLGSGFANNEEELQSLAQQALAHSSQLIV
|
58
|
+
DKSLKGWKEVEYEVVRDAYNNCITVCNMENFDPLGIHTGESIVVAPSQTLSDREYQMLRS
|
59
|
+
TALKVIRHFGVVGECNIQYALCPHSEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLA
|
60
|
+
LGLPLPDIKNSVTGNTTACFEPSLDYCVVKIPRWDLAKFVRVSKHIGSSMKSVGEVMAIG
|
61
|
+
RNFEEAFQKALRMVDSDVLGFDPDVVPLNKEQLAEQLSEPTDRRPFVIAAALQLGMSLRE
|
62
|
+
LHQLTNIDYWFLEKLERIILLQSLLTRNGSRTDAALLLKAKRFGFSDKQIAKYIKSTELA
|
63
|
+
VRHQRQEFGIRPHVKQIDTVAGEWPASTNYLYHTYNGSEHDVDFPGGHTIVVGSGVYRIG
|
64
|
+
SSVEFDWCAVGCLRELRKLQRPTIMINYNPETVSTDYDMCDRLYFEEISFEVVMDIYEME
|
65
|
+
NSEGIILSMGGQLPNNIAMDLHRQQAKVLGTSPESIDCAENRFKFSRMLDRKGILQPRWK
|
66
|
+
ELTNLQSAIEFCEEVGYPCLVRPSYVLSGAAMNVAYSNQDLETYLNAASEVSREHPVVIS
|
67
|
+
KFLTEAKEIDVDAVASDGRILCMAVSEHVENAGVHSGDATLVTPPQDLNAETLEAIKRIT
|
68
|
+
CDLASVLDVTGPFNMQLIAKNNELKVIECNVRVSRSFPFVSKTLDHDFVATATRAIVGLD
|
69
|
+
VEPLDVLHGVGKVGVKVPQFSFSRLAGADVQLGVEMASTGEVACFGDNRYEAYLKAMMST
|
70
|
+
GFQIPKNAVLLSIGSFKHKMELLPSIRDLAKMGYKLYASMGTGDFYAEHGVNVSIFMSVC
|
71
|
+
LLYIMQL
|
72
|
+
>BIB_DROME 362030490
|
73
|
+
MADESLHTVPLEHNIDYHIVTLFERLEAMRKDSHGGGHGVNNRLSSTLQAPKRSMQAEIR
|
74
|
+
TLEFWRSIISECLASFMYVFIVCGAAAGVGVGASVSSVLLATALASGLAMATLTQCFLHI
|
75
|
+
SGAHINPAVTLALCVVRSISPIRAAMYITAQCGGGIAGAALLYGVTVPGYQGNLQAAISH
|
76
|
+
SAALAAWERFGVEFILTFLVVLCYFVSTDPMKKFMGNSAASIGCAYSACCFVSMPYLNPA
|
77
|
+
RSLGPSFVLNKWDSHWVYWFGPLVGGMASGLVYEYIFNSRNRNLRHNKGSIDNDSSSIHS
|
78
|
+
EDELNYDMDMEKPNKYQQSQGTYPRGQSNGNGGGQAAGNGQHQAANMGQMPGVVANAGQG
|
79
|
+
NYCQNLYTAPPLSSKYDQQQEPLYGGTRSLYCRSPTLTRSNLNRSQSVYAKSNTAINRDI
|
80
|
+
VPRPGPLVPAQSLYPMRTQQQQQQQQQQQQQVAPAPQSSHLQNQNVQNQMQQRSESIYGM
|
81
|
+
RGSMRGQQQPIQQQQQQQQQQQLQQQQPNMGVQQQQMQPPPQMMSDPQQQPQGFQPVYGT
|
82
|
+
RTNPTPMDGNHKYDRRDPQQMYGVTGPRNRGQSAQSDDSSYGSYHGSAVTPPARHPSVEP
|
83
|
+
SPPPPPMLMYAPPPQPNAAHPQPIRTQSERKVSAPVVVSQPAACAVTYTTSQGSAVTAQQ
|
84
|
+
QQQQQQQQQQQQQQQQQQMMMQQQQQHYGMLPLRPN
|
data/test/data/test.gff3
ADDED
@@ -0,0 +1,5 @@
|
|
1
|
+
##gff-version 3
|
2
|
+
2R FlyMine gene 5866746 5868284 . + . ID=FBgn0000606
|
3
|
+
2R FlyMine exon 5866746 5867058 . + . ID=CG2328%3A1;Parent=FBgn0000606
|
4
|
+
2R FlyMine mRNA 5866746 5868284 . + . ID=FBtr0088390;Parent=FBgn0000606
|
5
|
+
2R FlyMine exon 5867130 5868284 . + . ID=CG2328%3A2;Parent=FBgn0000606
|
data/test/test_helper.rb
ADDED
@@ -0,0 +1,67 @@
|
|
1
|
+
$LOAD_PATH << File.expand_path( File.dirname(__FILE__) + '/../lib' )
|
2
|
+
require "rexml/document"
|
3
|
+
require "test/unit"
|
4
|
+
|
5
|
+
include Test::Unit::Assertions
|
6
|
+
|
7
|
+
def compare_xml(a, b)
|
8
|
+
require "rexml/document"
|
9
|
+
docA = REXML::Document.new(a.to_s)
|
10
|
+
docB = REXML::Document.new(b.to_s)
|
11
|
+
|
12
|
+
a_elems = docA.elements.to_a
|
13
|
+
b_elems = docB.elements.to_a
|
14
|
+
|
15
|
+
(0 ... a_elems.size).each do |idx|
|
16
|
+
compare_elements(a_elems[idx], b_elems[idx])
|
17
|
+
end
|
18
|
+
end
|
19
|
+
|
20
|
+
private
|
21
|
+
|
22
|
+
def fail_xml_compare(elemA, elemB, problem, e)
|
23
|
+
formatter = REXML::Formatters::Pretty.new
|
24
|
+
elemA_str = String.new
|
25
|
+
elemB_str = String.new
|
26
|
+
formatter.write(elemA, elemA_str)
|
27
|
+
formatter.write(elemB, elemB_str)
|
28
|
+
first_part = "#{elemA_str}\nis not equal to\n#{elemB_str}\n"
|
29
|
+
|
30
|
+
raise Test::Unit::AssertionFailedError, "#{first_part}because #{problem} - #{e.message}"
|
31
|
+
end
|
32
|
+
|
33
|
+
def compare_elements(elemA, elemB)
|
34
|
+
|
35
|
+
begin
|
36
|
+
assert_equal(elemA.name, elemB.name)
|
37
|
+
rescue Test::Unit::AssertionFailedError => e
|
38
|
+
fail_xml_compare(elemA, elemB, "names of element differ", e)
|
39
|
+
end
|
40
|
+
|
41
|
+
begin
|
42
|
+
assert_equal(elemA.attributes, elemB.attributes)
|
43
|
+
rescue Test::Unit::AssertionFailedError => e
|
44
|
+
fail_xml_compare(elemA, elemB, "attributes of element differ", e)
|
45
|
+
end
|
46
|
+
|
47
|
+
begin
|
48
|
+
assert_equal(elemA.text, elemB.text)
|
49
|
+
rescue Test::Unit::AssertionFailedError => e
|
50
|
+
fail_xml_compare(elemA, elemB, "text contents of element differ", e)
|
51
|
+
end
|
52
|
+
|
53
|
+
begin
|
54
|
+
assert_equal(elemA.elements.size, elemB.elements.size)
|
55
|
+
rescue Test::Unit::AssertionFailedError => e
|
56
|
+
fail_xml_compare(elemA, elemB, "number of children of element differ", e)
|
57
|
+
end
|
58
|
+
|
59
|
+
a_elems = elemA.elements.to_a
|
60
|
+
b_elems = elemB.elements.to_a
|
61
|
+
|
62
|
+
(0 ... a_elems.size).each do |idx|
|
63
|
+
compare_elements(a_elems[idx], b_elems[idx])
|
64
|
+
end
|
65
|
+
end
|
66
|
+
|
67
|
+
|
data/test/unit_tests.rb
ADDED
@@ -0,0 +1,112 @@
|
|
1
|
+
require File.dirname(__FILE__) + "/test_helper.rb"
|
2
|
+
|
3
|
+
require "intermine/bio"
|
4
|
+
require "test/unit"
|
5
|
+
|
6
|
+
class MockQuery
|
7
|
+
def service
|
8
|
+
return MockService.new
|
9
|
+
end
|
10
|
+
end
|
11
|
+
|
12
|
+
class MockService
|
13
|
+
def root
|
14
|
+
return "http://www.flymine.org/query/service"
|
15
|
+
end
|
16
|
+
end
|
17
|
+
|
18
|
+
module InterMine
|
19
|
+
|
20
|
+
module PathQuery
|
21
|
+
|
22
|
+
class Query
|
23
|
+
|
24
|
+
def results_reader
|
25
|
+
return InterMine::Results::ResultsReader.new(MockQuery.new)
|
26
|
+
end
|
27
|
+
|
28
|
+
end
|
29
|
+
|
30
|
+
end
|
31
|
+
|
32
|
+
module Results
|
33
|
+
|
34
|
+
class ResultsReader
|
35
|
+
|
36
|
+
attr_reader :uri
|
37
|
+
|
38
|
+
def initialize(mock_query)
|
39
|
+
@query = mock_query
|
40
|
+
end
|
41
|
+
|
42
|
+
def each_line(params)
|
43
|
+
f = File.new(File.dirname(__FILE__) + "/data/test.#{params[:format]}", "r")
|
44
|
+
f.each_line {|line| yield line}
|
45
|
+
end
|
46
|
+
|
47
|
+
def params(format)
|
48
|
+
return {:format => format}
|
49
|
+
end
|
50
|
+
|
51
|
+
unless ENV['LIVE_TESTING'] == "1"
|
52
|
+
def adjust_path(*args)
|
53
|
+
# disable, as it makes requests
|
54
|
+
end
|
55
|
+
end
|
56
|
+
|
57
|
+
end
|
58
|
+
end
|
59
|
+
end
|
60
|
+
|
61
|
+
class TestBio < Test::Unit::TestCase
|
62
|
+
|
63
|
+
def setup
|
64
|
+
@rr = InterMine::Results::ResultsReader.new(MockQuery.new)
|
65
|
+
end
|
66
|
+
|
67
|
+
def testServiceResolution
|
68
|
+
if ENV['LIVE_TESTING'] == "1"
|
69
|
+
@rr.adjust_path("bed")
|
70
|
+
assert_equal("/query/service/query/results/bed", @rr.uri.path)
|
71
|
+
@rr.adjust_path("gff3")
|
72
|
+
assert_equal("/query/service/query/results/gff3", @rr.uri.path)
|
73
|
+
@rr.adjust_path("fasta")
|
74
|
+
assert_equal("/query/service/query/results/fasta", @rr.uri.path)
|
75
|
+
end
|
76
|
+
end
|
77
|
+
|
78
|
+
def testBedParsing
|
79
|
+
assert_equal(InterMine::PathQuery::Query.new.bed, File.new(File.dirname(__FILE__) + "/data/test.bed", "r").read)
|
80
|
+
|
81
|
+
c = 0
|
82
|
+
header = InterMine::PathQuery::Query.new.bed {|b| c += 1}
|
83
|
+
assert_equal(4, c)
|
84
|
+
assert_match(/Source: FlyMine/, header)
|
85
|
+
end
|
86
|
+
|
87
|
+
def testGff3Parsing
|
88
|
+
assert_equal(InterMine::PathQuery::Query.new.gff3, File.new(File.dirname(__FILE__) + "/data/test.gff3", "r").read)
|
89
|
+
|
90
|
+
c = 0
|
91
|
+
header = InterMine::PathQuery::Query.new.gff3 {|g| c += 1}
|
92
|
+
assert_equal(4, c)
|
93
|
+
assert_match(/gff-version 3/, header)
|
94
|
+
end
|
95
|
+
|
96
|
+
def testFastaParsing
|
97
|
+
assert_equal(InterMine::PathQuery::Query.new.fasta, File.new(File.dirname(__FILE__) + "/data/test.fasta", "r").read)
|
98
|
+
|
99
|
+
c = 0
|
100
|
+
last = nil
|
101
|
+
InterMine::PathQuery::Query.new.fasta {|f| c += 1; last = f}
|
102
|
+
assert_equal(4, c)
|
103
|
+
assert_match(/BIB_DROME 362030490/, last)
|
104
|
+
end
|
105
|
+
|
106
|
+
end
|
107
|
+
|
108
|
+
|
109
|
+
|
110
|
+
|
111
|
+
|
112
|
+
|
metadata
ADDED
@@ -0,0 +1,146 @@
|
|
1
|
+
--- !ruby/object:Gem::Specification
|
2
|
+
name: intermine-bio
|
3
|
+
version: !ruby/object:Gem::Version
|
4
|
+
hash: 405
|
5
|
+
prerelease: false
|
6
|
+
segments:
|
7
|
+
- 0
|
8
|
+
- 98
|
9
|
+
- 1
|
10
|
+
version: 0.98.1
|
11
|
+
platform: ruby
|
12
|
+
authors:
|
13
|
+
- Alex Kalderimis
|
14
|
+
autorequire:
|
15
|
+
bindir: bin
|
16
|
+
cert_chain: []
|
17
|
+
|
18
|
+
date: 2011-08-02 00:00:00 +01:00
|
19
|
+
default_executable:
|
20
|
+
dependencies:
|
21
|
+
- !ruby/object:Gem::Dependency
|
22
|
+
name: intermine
|
23
|
+
prerelease: false
|
24
|
+
requirement: &id001 !ruby/object:Gem::Requirement
|
25
|
+
none: false
|
26
|
+
requirements:
|
27
|
+
- - ">="
|
28
|
+
- !ruby/object:Gem::Version
|
29
|
+
hash: 3
|
30
|
+
segments:
|
31
|
+
- 0
|
32
|
+
version: "0"
|
33
|
+
type: :runtime
|
34
|
+
version_requirements: *id001
|
35
|
+
description: |
|
36
|
+
= Biological Extensions to the InterMine Webservice Client Library
|
37
|
+
|
38
|
+
This library is a set of extensions to the InterMine Webservices client,
|
39
|
+
providing access for data in biological formats. It directly extends the
|
40
|
+
InterMine classes, providing extra methods to the Query class.
|
41
|
+
|
42
|
+
== Example
|
43
|
+
|
44
|
+
Get all sequences for proteins on "h", "r", "eve", "bib" and "zen":
|
45
|
+
|
46
|
+
require "rubygems"
|
47
|
+
require "intermine/service"
|
48
|
+
require "intermine/bio"
|
49
|
+
|
50
|
+
s = Service.new("www.flymine.org/query")
|
51
|
+
|
52
|
+
puts s.query("Gene").select("proteins").where(:symbol => %w{h r eve bib zen}).fasta
|
53
|
+
|
54
|
+
Process the locations of these genes one at a time:
|
55
|
+
|
56
|
+
s.query.select("Gene").where(:symbol => %w{h r eve bib zen}).bed do |line|
|
57
|
+
process line
|
58
|
+
end
|
59
|
+
|
60
|
+
== Who is this for?
|
61
|
+
|
62
|
+
InterMine data warehouses are typically constructed to hold
|
63
|
+
Biological data, and as this library facilitates programmatic
|
64
|
+
access to these data, this install is primarily aimed at
|
65
|
+
bioinformaticians. In particular, users of the following services
|
66
|
+
may find it especially useful:
|
67
|
+
* FlyMine (http://www.flymine.org/query)
|
68
|
+
* YeastMine (http://yeastmine.yeastgenome.org/yeastmine)
|
69
|
+
* RatMine (http://ratmine.mcw.edu/ratmine)
|
70
|
+
* modMine (http://intermine.modencode.org/release-23)
|
71
|
+
* metabolicMine (http://www.metabolicmine.org/beta)
|
72
|
+
|
73
|
+
These extensions are aimed at bioinformaticians looking to integrate
|
74
|
+
these sources of data into other workflows.
|
75
|
+
|
76
|
+
For details on constructing queries, see the intermine documentation.
|
77
|
+
|
78
|
+
== Support
|
79
|
+
|
80
|
+
Support is available on our development mailing list: dev@intermine.org
|
81
|
+
|
82
|
+
== License
|
83
|
+
|
84
|
+
This code is Open Source under the LGPL. Source code for all InterMine code
|
85
|
+
can be checked out from svn://subversion.flymine.org/flymine
|
86
|
+
|
87
|
+
email:
|
88
|
+
- dev@intermine.org
|
89
|
+
executables: []
|
90
|
+
|
91
|
+
extensions: []
|
92
|
+
|
93
|
+
extra_rdoc_files: []
|
94
|
+
|
95
|
+
files:
|
96
|
+
- lib/intermine/bio.rb
|
97
|
+
- test/data/test.bed
|
98
|
+
- test/data/test.fasta
|
99
|
+
- test/data/test.gff3
|
100
|
+
- test/test_helper.rb
|
101
|
+
- test/unit_tests.rb
|
102
|
+
- LICENCE
|
103
|
+
- Rakefile
|
104
|
+
- README.rdoc
|
105
|
+
- Gemfile
|
106
|
+
- contact_header.rdoc
|
107
|
+
has_rdoc: true
|
108
|
+
homepage: http://www.intermine.org
|
109
|
+
licenses:
|
110
|
+
- LGPL
|
111
|
+
post_install_message:
|
112
|
+
rdoc_options:
|
113
|
+
- --title
|
114
|
+
- Biological Extensions to the InterMine Webservice Client
|
115
|
+
- --main
|
116
|
+
- README.rdoc
|
117
|
+
- --line-numbers
|
118
|
+
require_paths:
|
119
|
+
- lib
|
120
|
+
required_ruby_version: !ruby/object:Gem::Requirement
|
121
|
+
none: false
|
122
|
+
requirements:
|
123
|
+
- - ">="
|
124
|
+
- !ruby/object:Gem::Version
|
125
|
+
hash: 3
|
126
|
+
segments:
|
127
|
+
- 0
|
128
|
+
version: "0"
|
129
|
+
required_rubygems_version: !ruby/object:Gem::Requirement
|
130
|
+
none: false
|
131
|
+
requirements:
|
132
|
+
- - ">="
|
133
|
+
- !ruby/object:Gem::Version
|
134
|
+
hash: 3
|
135
|
+
segments:
|
136
|
+
- 0
|
137
|
+
version: "0"
|
138
|
+
requirements: []
|
139
|
+
|
140
|
+
rubyforge_project: intermine-bio
|
141
|
+
rubygems_version: 1.3.7
|
142
|
+
signing_key:
|
143
|
+
specification_version: 3
|
144
|
+
summary: Biological Extensions for the InterMine Webservice Client Library
|
145
|
+
test_files:
|
146
|
+
- test/unit_tests.rb
|