intermine-bio 0.98.1
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- data/Gemfile +4 -0
- data/LICENCE +165 -0
- data/README.rdoc +50 -0
- data/Rakefile +62 -0
- data/contact_header.rdoc +10 -0
- data/lib/intermine/bio.rb +221 -0
- data/test/data/test.bed +8 -0
- data/test/data/test.fasta +84 -0
- data/test/data/test.gff3 +5 -0
- data/test/test_helper.rb +67 -0
- data/test/unit_tests.rb +112 -0
- metadata +146 -0
data/Gemfile
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data/LICENCE
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GNU LESSER GENERAL PUBLIC LICENSE
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Version 3, 29 June 2007
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Copyright (C) 2007 Free Software Foundation, Inc. <http://fsf.org/>
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Everyone is permitted to copy and distribute verbatim copies
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of this license document, but changing it is not allowed.
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This version of the GNU Lesser General Public License incorporates
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the terms and conditions of version 3 of the GNU General Public
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License, supplemented by the additional permissions listed below.
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0. Additional Definitions.
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As used herein, "this License" refers to version 3 of the GNU Lesser
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Library.
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data/README.rdoc
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= Biological Extensions to the InterMine Webservice Client Library
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This library is a set of extensions to the InterMine Webservices client,
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providing access for data in biological formats. It directly extends the
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InterMine classes, providing extra methods to the Query class.
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== Example
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Get all sequences for proteins on "h", "r", "eve", "bib" and "zen":
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require "rubygems"
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require "intermine/service"
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require "intermine/bio"
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s = Service.new("www.flymine.org/query")
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puts s.query("Gene").select("proteins").where(:symbol => %w{h r eve bib zen}).fasta
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Process the locations of these genes one at a time:
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s.query.select("Gene").where(:symbol => %w{h r eve bib zen}).bed do |line|
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process line
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end
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== Who is this for?
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InterMine data warehouses are typically constructed to hold
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Biological data, and as this library facilitates programmatic
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access to these data, this install is primarily aimed at
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bioinformaticians. In particular, users of the following services
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may find it especially useful:
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* FlyMine (http://www.flymine.org/query)
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* YeastMine (http://yeastmine.yeastgenome.org/yeastmine)
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* RatMine (http://ratmine.mcw.edu/ratmine)
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* modMine (http://intermine.modencode.org/release-23)
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* metabolicMine (http://www.metabolicmine.org/beta)
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These extensions are aimed at bioinformaticians looking to integrate
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these sources of data into other workflows.
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For details on constructing queries, see the intermine documentation.
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== Support
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Support is available on our development mailing list: dev@intermine.org
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== License
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This code is Open Source under the LGPL. Source code for all InterMine code
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can be checked out from svn://subversion.flymine.org/flymine
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data/Rakefile
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require 'rubygems'
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require 'rubygems/specification' unless defined?(Gem::Specification)
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require 'rake/testtask'
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require 'rake/gempackagetask'
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gem 'rdoc', '=2.1.0'
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require 'rdoc/rdoc'
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require 'rake/rdoctask'
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gem 'darkfish-rdoc'
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require 'darkfish-rdoc'
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def gemspec
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@gemspec ||= begin
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Gem::Specification.load(File.expand_path('intermine-bio.gemspec'))
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end
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end
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task :default => :test
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desc 'Start a console session'
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task :console do
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system 'irb -I lib -r rubygems -r intermine/service -r intermine/bio'
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end
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desc 'Displays the current version'
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task :version do
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puts "Current version: #{gemspec.version}"
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end
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desc 'Installs the gem locally'
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task :install => :package do
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sh "gem install pkg/#{gemspec.name}-#{gemspec.version}"
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end
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desc 'Release the gem'
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task :release => :package do
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sh "gem push pkg/#{gemspec.name}-#{gemspec.version}.gem"
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end
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Rake::GemPackageTask.new(gemspec) do |pkg|
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pkg.need_zip = true
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pkg.need_tar = true
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end
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Rake::TestTask.new do |t|
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t.libs << "test"
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t.test_files = FileList['test/unit_tests.rb']
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t.verbose = true
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t.warning = true
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end
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Rake::RDocTask.new do |t|
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t.title = 'Bio Extensions to the InterMine Webservice Client'
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t.rdoc_files.include 'README.rdoc'
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t.rdoc_files.include 'lib/**/*rb'
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t.main = 'README.rdoc'
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t.options += ['-SHN', '-f', 'darkfish']
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end
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data/contact_header.rdoc
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#
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# [author] Alex Kalderimis dev@intermine.org
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# [homepage] http://www.intermine.org
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# [Licence] Copyright (C) 2002-2011 FlyMine
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#
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# This code may be freely distributed and modified under the
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# terms of the GNU Lesser General Public Licence. This should
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# be distributed with the code. See the LICENSE file for more
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# information or http://www.gnu.org/copyleft/lesser.html.
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#
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require "net/http"
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# == Biologically specific Extensions to the InterMine Data-Warehousing Webservices
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#
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# These modules provide interfaces to the biologically specific elements of the
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# InterMine webservices. At present this consists of the ability to return results
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# from queries in Biologically specific formats (GFF3, UCSC-BED, FASTA). The
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# methods for accessing these formats provide mechanisms for iterating over the contents
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# in logical chunks.
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#
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#:include:contact_header.rdoc
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#
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module InterMine
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# The Metadata for these extensions
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#
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#:include:contact_header.rdoc
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#
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module Bio
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# The library version
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VERSION = "0.98.1"
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# The library name
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NAME = "intermine-bio"
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# The project's homepage
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HOMEPAGE = "http://www.intermine.org"
|
|
29
|
+
|
|
30
|
+
# The authors of this library
|
|
31
|
+
AUTHORS = ["Alex Kalderimis"]
|
|
32
|
+
|
|
33
|
+
# An email address to seek support at
|
|
34
|
+
EMAIL = ["dev@intermine.org"]
|
|
35
|
+
end
|
|
36
|
+
|
|
37
|
+
# Extensions to the PathQuery module.
|
|
38
|
+
#
|
|
39
|
+
#:include:contact_header.rdoc
|
|
40
|
+
#
|
|
41
|
+
module PathQuery
|
|
42
|
+
|
|
43
|
+
class BioError < RuntimeError
|
|
44
|
+
end
|
|
45
|
+
|
|
46
|
+
# Biologically specific Extensions to the Query class.
|
|
47
|
+
#
|
|
48
|
+
# These methods provide mechanisms for accessing results in Biologically
|
|
49
|
+
# appropriate formats, being at present GFF3, FASTA, and UCSC-BED.
|
|
50
|
+
#
|
|
51
|
+
# The methods provided here can be used to both return the data as a string,
|
|
52
|
+
# and to iterate over the data in logical chunks, approriate to the format in question.
|
|
53
|
+
#
|
|
54
|
+
#:include:contact_header.rdoc
|
|
55
|
+
#
|
|
56
|
+
class Query
|
|
57
|
+
|
|
58
|
+
# Return the results from this query as GFF3
|
|
59
|
+
#
|
|
60
|
+
# If a block is given, each line of the GFF3 will be
|
|
61
|
+
# yielded in turn, omitting any header lines, which are
|
|
62
|
+
# returned at the end of the iteration, otherwise
|
|
63
|
+
# the content of the GFF3 results will be returned as one string.
|
|
64
|
+
#
|
|
65
|
+
# header = query.gff3 do |line|
|
|
66
|
+
# process line
|
|
67
|
+
# end
|
|
68
|
+
#
|
|
69
|
+
# puts query.gff3
|
|
70
|
+
#
|
|
71
|
+
def gff3
|
|
72
|
+
if block_given?
|
|
73
|
+
header = ""
|
|
74
|
+
results_reader.each_gff3 do |gff3|
|
|
75
|
+
if gff3.start_with? "#"
|
|
76
|
+
header << gff3
|
|
77
|
+
else
|
|
78
|
+
yield gff3
|
|
79
|
+
end
|
|
80
|
+
end
|
|
81
|
+
return header
|
|
82
|
+
else
|
|
83
|
+
buffer = ""
|
|
84
|
+
results_reader.each_gff3 do |gff3|
|
|
85
|
+
buffer.concat(gff3)
|
|
86
|
+
end
|
|
87
|
+
return buffer
|
|
88
|
+
end
|
|
89
|
+
end
|
|
90
|
+
|
|
91
|
+
# Return the results from this query as FASTA
|
|
92
|
+
#
|
|
93
|
+
# If a block is given, each FASTA record will be yielded
|
|
94
|
+
# in turn, and the query will be returned, otherwise
|
|
95
|
+
# the content of the FASTA results will be returned as one string.
|
|
96
|
+
#
|
|
97
|
+
# query.fasta do |record|
|
|
98
|
+
# process record
|
|
99
|
+
# end
|
|
100
|
+
#
|
|
101
|
+
# puts query.fasta
|
|
102
|
+
#
|
|
103
|
+
def fasta # :yields: record
|
|
104
|
+
if block_given?
|
|
105
|
+
buffer = nil
|
|
106
|
+
results_reader.each_fasta do |line|
|
|
107
|
+
if line.start_with? ">"
|
|
108
|
+
yield buffer unless buffer.nil?
|
|
109
|
+
buffer = line
|
|
110
|
+
else
|
|
111
|
+
raise BioError, "Incorrect fasta - no header line" if buffer.nil?
|
|
112
|
+
buffer << line
|
|
113
|
+
end
|
|
114
|
+
end
|
|
115
|
+
yield buffer unless buffer.nil?
|
|
116
|
+
return self
|
|
117
|
+
else
|
|
118
|
+
buffer = ""
|
|
119
|
+
results_reader.each_fasta do |line|
|
|
120
|
+
buffer.concat(line)
|
|
121
|
+
end
|
|
122
|
+
return buffer
|
|
123
|
+
end
|
|
124
|
+
end
|
|
125
|
+
|
|
126
|
+
# Return the results from this query as BED
|
|
127
|
+
#
|
|
128
|
+
# If a block is given, each line of the BED results will be
|
|
129
|
+
# yielded in turn, and the header will be returned, otherwise
|
|
130
|
+
# the content of the BED results will be returned as one string.
|
|
131
|
+
#
|
|
132
|
+
# If the optional parameter is set to false, then the "chr" prefix on
|
|
133
|
+
# the chromosome id will be omitted.
|
|
134
|
+
#
|
|
135
|
+
# header = query.bed do |line|
|
|
136
|
+
# process line
|
|
137
|
+
# end
|
|
138
|
+
#
|
|
139
|
+
# puts query.bed(false)
|
|
140
|
+
#
|
|
141
|
+
def bed(ucscCompatible=true)
|
|
142
|
+
if block_given?
|
|
143
|
+
header = ""
|
|
144
|
+
results_reader.each_bed(ucscCompatible) do |bed|
|
|
145
|
+
if bed =~ /^\s*(#|track)/
|
|
146
|
+
header << bed
|
|
147
|
+
else
|
|
148
|
+
yield bed
|
|
149
|
+
end
|
|
150
|
+
end
|
|
151
|
+
return header
|
|
152
|
+
else
|
|
153
|
+
buffer = ""
|
|
154
|
+
results_reader.each_bed(ucscCompatible) do |bed|
|
|
155
|
+
buffer.concat(bed)
|
|
156
|
+
end
|
|
157
|
+
return buffer
|
|
158
|
+
end
|
|
159
|
+
end
|
|
160
|
+
end
|
|
161
|
+
end
|
|
162
|
+
|
|
163
|
+
# Extensions to the Result processing code
|
|
164
|
+
#
|
|
165
|
+
#:include:contact_header.rdoc
|
|
166
|
+
#
|
|
167
|
+
module Results
|
|
168
|
+
|
|
169
|
+
# Extensions to the ResultsReader object
|
|
170
|
+
#
|
|
171
|
+
# These methods provide mechanisms for accessing the results in raw
|
|
172
|
+
# format, and iterating over them line by line in a memory efficient
|
|
173
|
+
# manner. They are in no way content aware.
|
|
174
|
+
#
|
|
175
|
+
#:include:contact_header.rdoc
|
|
176
|
+
#
|
|
177
|
+
class ResultsReader
|
|
178
|
+
|
|
179
|
+
# The path to use to get the resource paths for the query variants
|
|
180
|
+
RESOURCE_PATH = "/check/"
|
|
181
|
+
|
|
182
|
+
# Yield results as GFF3
|
|
183
|
+
def each_gff3
|
|
184
|
+
adjust_path(:gff3)
|
|
185
|
+
each_line(params("gff3")) do |line|
|
|
186
|
+
yield line
|
|
187
|
+
end
|
|
188
|
+
end
|
|
189
|
+
|
|
190
|
+
# Yield results as fasta
|
|
191
|
+
def each_fasta
|
|
192
|
+
adjust_path(:fasta)
|
|
193
|
+
each_line(params("fasta")) do |line|
|
|
194
|
+
yield line
|
|
195
|
+
end
|
|
196
|
+
end
|
|
197
|
+
|
|
198
|
+
# Yield results as UCSC-BED
|
|
199
|
+
def each_bed(ucscCompatible=true)
|
|
200
|
+
adjust_path(:bed)
|
|
201
|
+
p = params("bed")
|
|
202
|
+
p["ucscCompatible"] = "no" unless ucscCompatible
|
|
203
|
+
each_line(p) do |line|
|
|
204
|
+
yield line
|
|
205
|
+
end
|
|
206
|
+
end
|
|
207
|
+
|
|
208
|
+
# Adjust the path of this query to suit the currently selected format.
|
|
209
|
+
def adjust_path(variant)
|
|
210
|
+
@resources ||= {}
|
|
211
|
+
root = @query.service.root
|
|
212
|
+
uri = URI.parse(root + RESOURCE_PATH + "query." + variant.to_s)
|
|
213
|
+
@resources[variant] = Net::HTTP.get(uri.host, uri.path)
|
|
214
|
+
path = @resources[variant]
|
|
215
|
+
@uri = URI.parse(root + path)
|
|
216
|
+
end
|
|
217
|
+
|
|
218
|
+
end
|
|
219
|
+
end
|
|
220
|
+
end
|
|
221
|
+
|
data/test/data/test.bed
ADDED
|
@@ -0,0 +1,8 @@
|
|
|
1
|
+
# UCSC BED format
|
|
2
|
+
# Source: FlyMine
|
|
3
|
+
# Genome Build: dm3
|
|
4
|
+
track name=FlyMine_newtpreview_Custom_Track description="FlyMine newtpreview Custom Track" useScore=0
|
|
5
|
+
chr2R 5866745 5868284 eve 0 +
|
|
6
|
+
chr2R 5866745 5867058 eve:1 0 +
|
|
7
|
+
chr2R 5866745 5868284 eve-RA 0 +
|
|
8
|
+
chr2R 5867129 5868284 eve:2 0 +
|
|
@@ -0,0 +1,84 @@
|
|
|
1
|
+
>ZEN1_DROME 362011840
|
|
2
|
+
MSSVMHYYPVHQAKVGSYSADPSEVKYSDLIYGHHHDVNPIGLPPNYNQMNSNPTTLNDH
|
|
3
|
+
CSPQHVHQQHVSSDENLPSQPNHDSQRVKLKRSRTAFTSVQLVELENEFKSNMYLYRTRR
|
|
4
|
+
IEIAQRLSLCERQVKIWFQNRRMKFKKDIQGHREPKSNAKLAQPQAEQSAHRGIVKRLMS
|
|
5
|
+
YSQDPREGTAAAEKRPMMAVAPVNPKPDYQASQKMKTEASTNNGMCSSADLSEILEHLAQ
|
|
6
|
+
TTAAPQVSTATSSTGTSTNSASSSSSGHYSYNVDLVLQSIKQDLEAAAQAWSKSKSAPIL
|
|
7
|
+
ATQSWHPSSQSQVPTSVHAAPSMNLSWGEPAAKSRKLSVNHMNPCVTSYNYPN
|
|
8
|
+
>PYR1_DROME 362022982
|
|
9
|
+
MASTDCYLALEDGTVLPGYSFGYVPSENESKVGFGGEVVFQTGMVGYTEALTDRSYSGQI
|
|
10
|
+
LVLTYPLIGNYGVPAPDEDEHGLPLHFEWMKGVVQATALVVGEVAEEAFHWRKWKTLPDW
|
|
11
|
+
LKQHKVPGIQDIDTRALTKKLREQGSMLGKIVYEKPPVEGLPKSSFVDPNVRNLAKECSV
|
|
12
|
+
KERQVYGNPNGKGPRIAILDCGLKLNQLRCLLQRGASVTLLPWSARLEDEQFDALFLSNG
|
|
13
|
+
PGNPESCDQIVQQVRKVIEEGQKPVFGICLGHQLLAKAIGCSTYKMKYGNRGHNLPCLHR
|
|
14
|
+
ATGRCLMTSQNHGYAVDLEQLPDGWSELFVNANDGTNEGIVHASKPYFSVQFHPEHHAGP
|
|
15
|
+
QDTEFLFDVFMESIQQKDLTIPQLIEQRLRPTTPAIDSAPVMPRKVLILGSGGLSIGQAG
|
|
16
|
+
EFDYSGSQAIKAMRESNIQTVLINPNIATVQTSKGMADKCYFLPLTPHYVEQVIKSERPN
|
|
17
|
+
GVLLTFGGQTALNCGVQLERAGVFSKYNVRILGTPIQSIIETEDRKLFAERVNEIGEQVA
|
|
18
|
+
PSEAVYSVAQALDAASRLGYPVMARAAFSLGGLGSGFANNEEELQSLAQQALAHSSQLIV
|
|
19
|
+
DKSLKGWKEVEYEVVRDAYNNCITVCNMENFDPLGIHTGESIVVAPSQTLSDREYQMLRS
|
|
20
|
+
TALKVIRHFGVVGECNIQYALCPHSEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLA
|
|
21
|
+
LGLPLPDIKNSVTGNTTACFEPSLDYCVVKIPRWDLAKFVRVSKHIGSSMKSVGEVMAIG
|
|
22
|
+
RNFEEAFQKALRMVDSDVLGFDPDVVPLNKEQLAEQLSEPTDRRPFVIAAALQLGMSLRE
|
|
23
|
+
LHQLTNIDYWFLEKLERIILLQSLLTRNGSRTDAALLLKAKRFGFSDKQIAKYIKSTELA
|
|
24
|
+
VRHQRQEFGIRPHVKQIDTVAGEWPASTNYLYHTYNGSEHDVDFPGGHTIVVGSGVYRIG
|
|
25
|
+
SSVEFDWCAVGCLRELRKLQRPTIMINYNPETVSTDYDMCDRLYFEEISFEVVMDIYEME
|
|
26
|
+
NSEGIILSMGGQLPNNIAMDLHRQQAKVLGTSPESIDCAENRFKFSRMLDRKGILQPRWK
|
|
27
|
+
ELTNLQSAIEFCEEVGYPCLVRPSYVLSGAAMNVAYSNQDLETYLNAASEVSREHPVVIS
|
|
28
|
+
KFLTEAKEIDVDAVASDGRILCMAVSEHVENAGVHSGDATLVTPPQDLNAETLEAIKRIT
|
|
29
|
+
CDLASVLDVTGPFNMQLIAKNNELKVIECNVRVSRSFPFVSKTLDHDFVATATRAIVGLD
|
|
30
|
+
VEPLDVLHGVGKVGVKVPQFSFSRLAGADVQLGVEMASTGEVACFGDNRYEAYLKAMMST
|
|
31
|
+
GFQIPKNAVLLSIGSFKHKMELLPSIRDLAKMGYKLYASMGTGDFYAEHGVNVESVQWTF
|
|
32
|
+
DKTTPDDINGELRHLAEFLANKQFDLVINLPMSGGGARRVSSFMTHGYRTRRLAVDYSIP
|
|
33
|
+
LVTDVKCTKLLVESMRMNGGKPPMKTHTDCMTSRRIVKLPGFIDVHVHLREPGATHKEDF
|
|
34
|
+
ASGTAAALAGGVTLVCAMPNTNPSIVDRETFTQFQELAKAGARCDYALYVGASDDNWAQV
|
|
35
|
+
NELASHACGLKMYLNDTFGTLKLSDMTSWQRHLSHWPKRSPIVCHAERQSTAAVIMLAHL
|
|
36
|
+
LDRSVHICHVARKEEIQLIRSAKEKGVKVTCEVCPHHLFLSTKDVERLGHGMSEVRPLLC
|
|
37
|
+
SPEDQEALWENIDYIDVFATDHAPHTLAEKRSERPPPGFPGVETILPLLLQAVHEGRLTM
|
|
38
|
+
EDIKRKFHRNPKIIFNLPDQAQTYVEVDLDEEWTITGNEMKSKSGWTPFEGTKVKGRVHR
|
|
39
|
+
VVLRGEVAFVDGQVLVQPGFGQNVRPKQSPLASEASQDLLPSDNDANDTFTRLLTSEGPG
|
|
40
|
+
GGVHGISTKVHFVDGANFLRPNSPSPRIRLDSASNTTLREYLQRTTNSNPVAHSLMGKHI
|
|
41
|
+
LAVDMFNKDHLNDIFNLAQLLKLRGTKDRPVDELLPGKIMASVFYEVSTRTQCSFAAAML
|
|
42
|
+
RLGGRVISMDNITSSVKKGESLEDSIKVVSSYADVVVLRHPSPGAVARAATFSRKPLINA
|
|
43
|
+
GDGVGEHPTQALLDIFTIREEFGTVNGLTITMVGDLKNGRTVHSLARLLTLYNVNLQYVA
|
|
44
|
+
PNSLQMPDEVVQFVHQRGVKQLFARDLKNVLPDTDVLYMTRIQRERFDNVEDYEKCCGHL
|
|
45
|
+
VLTPEHMMRAKKRSIVLHPLPRLNEISREIDSDPRAAYFRQAEYGMYIRMALLAMVVGGR
|
|
46
|
+
NTAL
|
|
47
|
+
>FBpp0088674 362022983
|
|
48
|
+
MASTDCYLALEDGTVLPGYSFGYVPSENESKVGFGGEVVFQTGMVGYTEALTDRSYSGQI
|
|
49
|
+
LVLTYPLIGNYGVPAPDEDEHGLPLHFEWMKGVVQATALVVGEVAEEAFHWRKWKTLPDW
|
|
50
|
+
LKQHKVPGIQDIDTRALTKKLREQGSMLGKIVYEKPPVEGLPKSSFVDPNVRNLAKECSV
|
|
51
|
+
KERQVYGNPNGKGPRIAILDCGLKLNQLRCLLQRGASVTLLPWSARLEDEQFDALFLSNG
|
|
52
|
+
PGNPESCDQIVQQVRKVIEEGQKPVFGICLGHQLLAKAIGCSTYKMKYGNRGHNLPCLHR
|
|
53
|
+
ATGRCLMTSQNHGYAVDLEQLPDGWSELFVNANDGTNEGIVHASKPYFSVQFHPEHHAGP
|
|
54
|
+
QDTEFLFDVFMESIQQKDLTIPQLIEQRLRPTTPAIDSAPVMPRKVLILGSGGLSIGQAG
|
|
55
|
+
EFDYSGSQAIKAMRESNIQTVLINPNIATVQTSKGMADKCYFLPLTPHYVEQVIKSERPN
|
|
56
|
+
GVLLTFGGQTALNCGVQLERAGVFSKYNVRILGTPIQSIIETEDRKLFAERVNEIGEQVA
|
|
57
|
+
PSEAVYSVAQALDAASRLGYPVMARAAFSLGGLGSGFANNEEELQSLAQQALAHSSQLIV
|
|
58
|
+
DKSLKGWKEVEYEVVRDAYNNCITVCNMENFDPLGIHTGESIVVAPSQTLSDREYQMLRS
|
|
59
|
+
TALKVIRHFGVVGECNIQYALCPHSEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLA
|
|
60
|
+
LGLPLPDIKNSVTGNTTACFEPSLDYCVVKIPRWDLAKFVRVSKHIGSSMKSVGEVMAIG
|
|
61
|
+
RNFEEAFQKALRMVDSDVLGFDPDVVPLNKEQLAEQLSEPTDRRPFVIAAALQLGMSLRE
|
|
62
|
+
LHQLTNIDYWFLEKLERIILLQSLLTRNGSRTDAALLLKAKRFGFSDKQIAKYIKSTELA
|
|
63
|
+
VRHQRQEFGIRPHVKQIDTVAGEWPASTNYLYHTYNGSEHDVDFPGGHTIVVGSGVYRIG
|
|
64
|
+
SSVEFDWCAVGCLRELRKLQRPTIMINYNPETVSTDYDMCDRLYFEEISFEVVMDIYEME
|
|
65
|
+
NSEGIILSMGGQLPNNIAMDLHRQQAKVLGTSPESIDCAENRFKFSRMLDRKGILQPRWK
|
|
66
|
+
ELTNLQSAIEFCEEVGYPCLVRPSYVLSGAAMNVAYSNQDLETYLNAASEVSREHPVVIS
|
|
67
|
+
KFLTEAKEIDVDAVASDGRILCMAVSEHVENAGVHSGDATLVTPPQDLNAETLEAIKRIT
|
|
68
|
+
CDLASVLDVTGPFNMQLIAKNNELKVIECNVRVSRSFPFVSKTLDHDFVATATRAIVGLD
|
|
69
|
+
VEPLDVLHGVGKVGVKVPQFSFSRLAGADVQLGVEMASTGEVACFGDNRYEAYLKAMMST
|
|
70
|
+
GFQIPKNAVLLSIGSFKHKMELLPSIRDLAKMGYKLYASMGTGDFYAEHGVNVSIFMSVC
|
|
71
|
+
LLYIMQL
|
|
72
|
+
>BIB_DROME 362030490
|
|
73
|
+
MADESLHTVPLEHNIDYHIVTLFERLEAMRKDSHGGGHGVNNRLSSTLQAPKRSMQAEIR
|
|
74
|
+
TLEFWRSIISECLASFMYVFIVCGAAAGVGVGASVSSVLLATALASGLAMATLTQCFLHI
|
|
75
|
+
SGAHINPAVTLALCVVRSISPIRAAMYITAQCGGGIAGAALLYGVTVPGYQGNLQAAISH
|
|
76
|
+
SAALAAWERFGVEFILTFLVVLCYFVSTDPMKKFMGNSAASIGCAYSACCFVSMPYLNPA
|
|
77
|
+
RSLGPSFVLNKWDSHWVYWFGPLVGGMASGLVYEYIFNSRNRNLRHNKGSIDNDSSSIHS
|
|
78
|
+
EDELNYDMDMEKPNKYQQSQGTYPRGQSNGNGGGQAAGNGQHQAANMGQMPGVVANAGQG
|
|
79
|
+
NYCQNLYTAPPLSSKYDQQQEPLYGGTRSLYCRSPTLTRSNLNRSQSVYAKSNTAINRDI
|
|
80
|
+
VPRPGPLVPAQSLYPMRTQQQQQQQQQQQQQVAPAPQSSHLQNQNVQNQMQQRSESIYGM
|
|
81
|
+
RGSMRGQQQPIQQQQQQQQQQQLQQQQPNMGVQQQQMQPPPQMMSDPQQQPQGFQPVYGT
|
|
82
|
+
RTNPTPMDGNHKYDRRDPQQMYGVTGPRNRGQSAQSDDSSYGSYHGSAVTPPARHPSVEP
|
|
83
|
+
SPPPPPMLMYAPPPQPNAAHPQPIRTQSERKVSAPVVVSQPAACAVTYTTSQGSAVTAQQ
|
|
84
|
+
QQQQQQQQQQQQQQQQQQMMMQQQQQHYGMLPLRPN
|
data/test/data/test.gff3
ADDED
|
@@ -0,0 +1,5 @@
|
|
|
1
|
+
##gff-version 3
|
|
2
|
+
2R FlyMine gene 5866746 5868284 . + . ID=FBgn0000606
|
|
3
|
+
2R FlyMine exon 5866746 5867058 . + . ID=CG2328%3A1;Parent=FBgn0000606
|
|
4
|
+
2R FlyMine mRNA 5866746 5868284 . + . ID=FBtr0088390;Parent=FBgn0000606
|
|
5
|
+
2R FlyMine exon 5867130 5868284 . + . ID=CG2328%3A2;Parent=FBgn0000606
|
data/test/test_helper.rb
ADDED
|
@@ -0,0 +1,67 @@
|
|
|
1
|
+
$LOAD_PATH << File.expand_path( File.dirname(__FILE__) + '/../lib' )
|
|
2
|
+
require "rexml/document"
|
|
3
|
+
require "test/unit"
|
|
4
|
+
|
|
5
|
+
include Test::Unit::Assertions
|
|
6
|
+
|
|
7
|
+
def compare_xml(a, b)
|
|
8
|
+
require "rexml/document"
|
|
9
|
+
docA = REXML::Document.new(a.to_s)
|
|
10
|
+
docB = REXML::Document.new(b.to_s)
|
|
11
|
+
|
|
12
|
+
a_elems = docA.elements.to_a
|
|
13
|
+
b_elems = docB.elements.to_a
|
|
14
|
+
|
|
15
|
+
(0 ... a_elems.size).each do |idx|
|
|
16
|
+
compare_elements(a_elems[idx], b_elems[idx])
|
|
17
|
+
end
|
|
18
|
+
end
|
|
19
|
+
|
|
20
|
+
private
|
|
21
|
+
|
|
22
|
+
def fail_xml_compare(elemA, elemB, problem, e)
|
|
23
|
+
formatter = REXML::Formatters::Pretty.new
|
|
24
|
+
elemA_str = String.new
|
|
25
|
+
elemB_str = String.new
|
|
26
|
+
formatter.write(elemA, elemA_str)
|
|
27
|
+
formatter.write(elemB, elemB_str)
|
|
28
|
+
first_part = "#{elemA_str}\nis not equal to\n#{elemB_str}\n"
|
|
29
|
+
|
|
30
|
+
raise Test::Unit::AssertionFailedError, "#{first_part}because #{problem} - #{e.message}"
|
|
31
|
+
end
|
|
32
|
+
|
|
33
|
+
def compare_elements(elemA, elemB)
|
|
34
|
+
|
|
35
|
+
begin
|
|
36
|
+
assert_equal(elemA.name, elemB.name)
|
|
37
|
+
rescue Test::Unit::AssertionFailedError => e
|
|
38
|
+
fail_xml_compare(elemA, elemB, "names of element differ", e)
|
|
39
|
+
end
|
|
40
|
+
|
|
41
|
+
begin
|
|
42
|
+
assert_equal(elemA.attributes, elemB.attributes)
|
|
43
|
+
rescue Test::Unit::AssertionFailedError => e
|
|
44
|
+
fail_xml_compare(elemA, elemB, "attributes of element differ", e)
|
|
45
|
+
end
|
|
46
|
+
|
|
47
|
+
begin
|
|
48
|
+
assert_equal(elemA.text, elemB.text)
|
|
49
|
+
rescue Test::Unit::AssertionFailedError => e
|
|
50
|
+
fail_xml_compare(elemA, elemB, "text contents of element differ", e)
|
|
51
|
+
end
|
|
52
|
+
|
|
53
|
+
begin
|
|
54
|
+
assert_equal(elemA.elements.size, elemB.elements.size)
|
|
55
|
+
rescue Test::Unit::AssertionFailedError => e
|
|
56
|
+
fail_xml_compare(elemA, elemB, "number of children of element differ", e)
|
|
57
|
+
end
|
|
58
|
+
|
|
59
|
+
a_elems = elemA.elements.to_a
|
|
60
|
+
b_elems = elemB.elements.to_a
|
|
61
|
+
|
|
62
|
+
(0 ... a_elems.size).each do |idx|
|
|
63
|
+
compare_elements(a_elems[idx], b_elems[idx])
|
|
64
|
+
end
|
|
65
|
+
end
|
|
66
|
+
|
|
67
|
+
|
data/test/unit_tests.rb
ADDED
|
@@ -0,0 +1,112 @@
|
|
|
1
|
+
require File.dirname(__FILE__) + "/test_helper.rb"
|
|
2
|
+
|
|
3
|
+
require "intermine/bio"
|
|
4
|
+
require "test/unit"
|
|
5
|
+
|
|
6
|
+
class MockQuery
|
|
7
|
+
def service
|
|
8
|
+
return MockService.new
|
|
9
|
+
end
|
|
10
|
+
end
|
|
11
|
+
|
|
12
|
+
class MockService
|
|
13
|
+
def root
|
|
14
|
+
return "http://www.flymine.org/query/service"
|
|
15
|
+
end
|
|
16
|
+
end
|
|
17
|
+
|
|
18
|
+
module InterMine
|
|
19
|
+
|
|
20
|
+
module PathQuery
|
|
21
|
+
|
|
22
|
+
class Query
|
|
23
|
+
|
|
24
|
+
def results_reader
|
|
25
|
+
return InterMine::Results::ResultsReader.new(MockQuery.new)
|
|
26
|
+
end
|
|
27
|
+
|
|
28
|
+
end
|
|
29
|
+
|
|
30
|
+
end
|
|
31
|
+
|
|
32
|
+
module Results
|
|
33
|
+
|
|
34
|
+
class ResultsReader
|
|
35
|
+
|
|
36
|
+
attr_reader :uri
|
|
37
|
+
|
|
38
|
+
def initialize(mock_query)
|
|
39
|
+
@query = mock_query
|
|
40
|
+
end
|
|
41
|
+
|
|
42
|
+
def each_line(params)
|
|
43
|
+
f = File.new(File.dirname(__FILE__) + "/data/test.#{params[:format]}", "r")
|
|
44
|
+
f.each_line {|line| yield line}
|
|
45
|
+
end
|
|
46
|
+
|
|
47
|
+
def params(format)
|
|
48
|
+
return {:format => format}
|
|
49
|
+
end
|
|
50
|
+
|
|
51
|
+
unless ENV['LIVE_TESTING'] == "1"
|
|
52
|
+
def adjust_path(*args)
|
|
53
|
+
# disable, as it makes requests
|
|
54
|
+
end
|
|
55
|
+
end
|
|
56
|
+
|
|
57
|
+
end
|
|
58
|
+
end
|
|
59
|
+
end
|
|
60
|
+
|
|
61
|
+
class TestBio < Test::Unit::TestCase
|
|
62
|
+
|
|
63
|
+
def setup
|
|
64
|
+
@rr = InterMine::Results::ResultsReader.new(MockQuery.new)
|
|
65
|
+
end
|
|
66
|
+
|
|
67
|
+
def testServiceResolution
|
|
68
|
+
if ENV['LIVE_TESTING'] == "1"
|
|
69
|
+
@rr.adjust_path("bed")
|
|
70
|
+
assert_equal("/query/service/query/results/bed", @rr.uri.path)
|
|
71
|
+
@rr.adjust_path("gff3")
|
|
72
|
+
assert_equal("/query/service/query/results/gff3", @rr.uri.path)
|
|
73
|
+
@rr.adjust_path("fasta")
|
|
74
|
+
assert_equal("/query/service/query/results/fasta", @rr.uri.path)
|
|
75
|
+
end
|
|
76
|
+
end
|
|
77
|
+
|
|
78
|
+
def testBedParsing
|
|
79
|
+
assert_equal(InterMine::PathQuery::Query.new.bed, File.new(File.dirname(__FILE__) + "/data/test.bed", "r").read)
|
|
80
|
+
|
|
81
|
+
c = 0
|
|
82
|
+
header = InterMine::PathQuery::Query.new.bed {|b| c += 1}
|
|
83
|
+
assert_equal(4, c)
|
|
84
|
+
assert_match(/Source: FlyMine/, header)
|
|
85
|
+
end
|
|
86
|
+
|
|
87
|
+
def testGff3Parsing
|
|
88
|
+
assert_equal(InterMine::PathQuery::Query.new.gff3, File.new(File.dirname(__FILE__) + "/data/test.gff3", "r").read)
|
|
89
|
+
|
|
90
|
+
c = 0
|
|
91
|
+
header = InterMine::PathQuery::Query.new.gff3 {|g| c += 1}
|
|
92
|
+
assert_equal(4, c)
|
|
93
|
+
assert_match(/gff-version 3/, header)
|
|
94
|
+
end
|
|
95
|
+
|
|
96
|
+
def testFastaParsing
|
|
97
|
+
assert_equal(InterMine::PathQuery::Query.new.fasta, File.new(File.dirname(__FILE__) + "/data/test.fasta", "r").read)
|
|
98
|
+
|
|
99
|
+
c = 0
|
|
100
|
+
last = nil
|
|
101
|
+
InterMine::PathQuery::Query.new.fasta {|f| c += 1; last = f}
|
|
102
|
+
assert_equal(4, c)
|
|
103
|
+
assert_match(/BIB_DROME 362030490/, last)
|
|
104
|
+
end
|
|
105
|
+
|
|
106
|
+
end
|
|
107
|
+
|
|
108
|
+
|
|
109
|
+
|
|
110
|
+
|
|
111
|
+
|
|
112
|
+
|
metadata
ADDED
|
@@ -0,0 +1,146 @@
|
|
|
1
|
+
--- !ruby/object:Gem::Specification
|
|
2
|
+
name: intermine-bio
|
|
3
|
+
version: !ruby/object:Gem::Version
|
|
4
|
+
hash: 405
|
|
5
|
+
prerelease: false
|
|
6
|
+
segments:
|
|
7
|
+
- 0
|
|
8
|
+
- 98
|
|
9
|
+
- 1
|
|
10
|
+
version: 0.98.1
|
|
11
|
+
platform: ruby
|
|
12
|
+
authors:
|
|
13
|
+
- Alex Kalderimis
|
|
14
|
+
autorequire:
|
|
15
|
+
bindir: bin
|
|
16
|
+
cert_chain: []
|
|
17
|
+
|
|
18
|
+
date: 2011-08-02 00:00:00 +01:00
|
|
19
|
+
default_executable:
|
|
20
|
+
dependencies:
|
|
21
|
+
- !ruby/object:Gem::Dependency
|
|
22
|
+
name: intermine
|
|
23
|
+
prerelease: false
|
|
24
|
+
requirement: &id001 !ruby/object:Gem::Requirement
|
|
25
|
+
none: false
|
|
26
|
+
requirements:
|
|
27
|
+
- - ">="
|
|
28
|
+
- !ruby/object:Gem::Version
|
|
29
|
+
hash: 3
|
|
30
|
+
segments:
|
|
31
|
+
- 0
|
|
32
|
+
version: "0"
|
|
33
|
+
type: :runtime
|
|
34
|
+
version_requirements: *id001
|
|
35
|
+
description: |
|
|
36
|
+
= Biological Extensions to the InterMine Webservice Client Library
|
|
37
|
+
|
|
38
|
+
This library is a set of extensions to the InterMine Webservices client,
|
|
39
|
+
providing access for data in biological formats. It directly extends the
|
|
40
|
+
InterMine classes, providing extra methods to the Query class.
|
|
41
|
+
|
|
42
|
+
== Example
|
|
43
|
+
|
|
44
|
+
Get all sequences for proteins on "h", "r", "eve", "bib" and "zen":
|
|
45
|
+
|
|
46
|
+
require "rubygems"
|
|
47
|
+
require "intermine/service"
|
|
48
|
+
require "intermine/bio"
|
|
49
|
+
|
|
50
|
+
s = Service.new("www.flymine.org/query")
|
|
51
|
+
|
|
52
|
+
puts s.query("Gene").select("proteins").where(:symbol => %w{h r eve bib zen}).fasta
|
|
53
|
+
|
|
54
|
+
Process the locations of these genes one at a time:
|
|
55
|
+
|
|
56
|
+
s.query.select("Gene").where(:symbol => %w{h r eve bib zen}).bed do |line|
|
|
57
|
+
process line
|
|
58
|
+
end
|
|
59
|
+
|
|
60
|
+
== Who is this for?
|
|
61
|
+
|
|
62
|
+
InterMine data warehouses are typically constructed to hold
|
|
63
|
+
Biological data, and as this library facilitates programmatic
|
|
64
|
+
access to these data, this install is primarily aimed at
|
|
65
|
+
bioinformaticians. In particular, users of the following services
|
|
66
|
+
may find it especially useful:
|
|
67
|
+
* FlyMine (http://www.flymine.org/query)
|
|
68
|
+
* YeastMine (http://yeastmine.yeastgenome.org/yeastmine)
|
|
69
|
+
* RatMine (http://ratmine.mcw.edu/ratmine)
|
|
70
|
+
* modMine (http://intermine.modencode.org/release-23)
|
|
71
|
+
* metabolicMine (http://www.metabolicmine.org/beta)
|
|
72
|
+
|
|
73
|
+
These extensions are aimed at bioinformaticians looking to integrate
|
|
74
|
+
these sources of data into other workflows.
|
|
75
|
+
|
|
76
|
+
For details on constructing queries, see the intermine documentation.
|
|
77
|
+
|
|
78
|
+
== Support
|
|
79
|
+
|
|
80
|
+
Support is available on our development mailing list: dev@intermine.org
|
|
81
|
+
|
|
82
|
+
== License
|
|
83
|
+
|
|
84
|
+
This code is Open Source under the LGPL. Source code for all InterMine code
|
|
85
|
+
can be checked out from svn://subversion.flymine.org/flymine
|
|
86
|
+
|
|
87
|
+
email:
|
|
88
|
+
- dev@intermine.org
|
|
89
|
+
executables: []
|
|
90
|
+
|
|
91
|
+
extensions: []
|
|
92
|
+
|
|
93
|
+
extra_rdoc_files: []
|
|
94
|
+
|
|
95
|
+
files:
|
|
96
|
+
- lib/intermine/bio.rb
|
|
97
|
+
- test/data/test.bed
|
|
98
|
+
- test/data/test.fasta
|
|
99
|
+
- test/data/test.gff3
|
|
100
|
+
- test/test_helper.rb
|
|
101
|
+
- test/unit_tests.rb
|
|
102
|
+
- LICENCE
|
|
103
|
+
- Rakefile
|
|
104
|
+
- README.rdoc
|
|
105
|
+
- Gemfile
|
|
106
|
+
- contact_header.rdoc
|
|
107
|
+
has_rdoc: true
|
|
108
|
+
homepage: http://www.intermine.org
|
|
109
|
+
licenses:
|
|
110
|
+
- LGPL
|
|
111
|
+
post_install_message:
|
|
112
|
+
rdoc_options:
|
|
113
|
+
- --title
|
|
114
|
+
- Biological Extensions to the InterMine Webservice Client
|
|
115
|
+
- --main
|
|
116
|
+
- README.rdoc
|
|
117
|
+
- --line-numbers
|
|
118
|
+
require_paths:
|
|
119
|
+
- lib
|
|
120
|
+
required_ruby_version: !ruby/object:Gem::Requirement
|
|
121
|
+
none: false
|
|
122
|
+
requirements:
|
|
123
|
+
- - ">="
|
|
124
|
+
- !ruby/object:Gem::Version
|
|
125
|
+
hash: 3
|
|
126
|
+
segments:
|
|
127
|
+
- 0
|
|
128
|
+
version: "0"
|
|
129
|
+
required_rubygems_version: !ruby/object:Gem::Requirement
|
|
130
|
+
none: false
|
|
131
|
+
requirements:
|
|
132
|
+
- - ">="
|
|
133
|
+
- !ruby/object:Gem::Version
|
|
134
|
+
hash: 3
|
|
135
|
+
segments:
|
|
136
|
+
- 0
|
|
137
|
+
version: "0"
|
|
138
|
+
requirements: []
|
|
139
|
+
|
|
140
|
+
rubyforge_project: intermine-bio
|
|
141
|
+
rubygems_version: 1.3.7
|
|
142
|
+
signing_key:
|
|
143
|
+
specification_version: 3
|
|
144
|
+
summary: Biological Extensions for the InterMine Webservice Client Library
|
|
145
|
+
test_files:
|
|
146
|
+
- test/unit_tests.rb
|