htslib 0.0.1 → 0.0.5
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- checksums.yaml +4 -4
- data/README.md +68 -17
- data/lib/hts/bam/cigar.rb +9 -6
- data/lib/hts/bam/flag.rb +93 -0
- data/lib/hts/bam/header.rb +12 -6
- data/lib/hts/bam/record.rb +195 -0
- data/lib/hts/bam.rb +67 -32
- data/lib/hts/bcf/format.rb +52 -0
- data/lib/hts/bcf/header.rb +19 -0
- data/lib/hts/bcf/info.rb +93 -0
- data/lib/hts/bcf/record.rb +110 -0
- data/lib/hts/bcf.rb +73 -0
- data/lib/hts/faidx.rb +59 -0
- data/lib/hts/ffi_ext/README.md +8 -0
- data/lib/hts/ffi_ext/struct.rb +45 -0
- data/lib/hts/{ffi → libhts}/bgzf.rb +2 -2
- data/lib/hts/{ffi → libhts}/constants.rb +144 -76
- data/lib/hts/{ffi → libhts}/faidx.rb +1 -1
- data/lib/hts/{ffi → libhts}/hfile.rb +2 -2
- data/lib/hts/{ffi → libhts}/hts.rb +9 -3
- data/lib/hts/{ffi → libhts}/kfunc.rb +1 -1
- data/lib/hts/{ffi → libhts}/sam.rb +60 -30
- data/lib/hts/{ffi → libhts}/tbx.rb +1 -1
- data/lib/hts/{ffi → libhts}/vcf.rb +215 -12
- data/lib/hts/libhts.rb +33 -0
- data/lib/hts/tabix.rb +28 -0
- data/lib/hts/version.rb +1 -1
- data/lib/htslib.rb +32 -17
- metadata +49 -28
- data/lib/hts/bam/alignment.rb +0 -156
- data/lib/hts/fai.rb +0 -18
- data/lib/hts/ffi.rb +0 -43
- data/lib/hts/tbx.rb +0 -16
- data/lib/hts/vcf.rb +0 -32
checksums.yaml
CHANGED
@@ -1,7 +1,7 @@
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---
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SHA256:
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metadata.gz:
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data.tar.gz:
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metadata.gz: 3eb6fbb228a9fa0642c55cdfa39d5189b311df191725954ae46b8dda135dc8c7
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data.tar.gz: c90ad39aa4919cefa56e534a706bf9659bba9c6ad69eb374491092ce16ae93f6
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SHA512:
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metadata.gz:
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data.tar.gz:
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+
metadata.gz: 392ea7c6acb5b86e9e1c1a29f066049c54954edfd277be30541aee433a7349f9d74df09e663ec0269f30d6b87ff47d66a4a668470d3aa536e25a2058cf963546
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data.tar.gz: 9cc235f8876b8fd4339593eb84a0f2cb8b605b9c82b624cd0f97691b4109a672f55ae90447bba17fb5efcb8257279ad8c2d87234bca390b1624c7aa8f9a7014e
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data/README.md
CHANGED
@@ -1,15 +1,26 @@
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-
#
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# ruby-htslib
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[![Gem Version](https://badge.fury.io/rb/htslib.svg)](https://badge.fury.io/rb/htslib)
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![CI](https://github.com/kojix2/ruby-htslib/workflows/CI/badge.svg)
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[![The MIT License](https://img.shields.io/badge/license-MIT-blue.svg)](LICENSE.txt)
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[![DOI](https://zenodo.org/badge/247078205.svg)](https://zenodo.org/badge/latestdoi/247078205)
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[![Docs Stable](https://img.shields.io/badge/docs-stable-blue.svg)](https://rubydoc.info/gems/htslib)
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-
:dna: [HTSlib](https://github.com/samtools/htslib) -
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:dna: [HTSlib](https://github.com/samtools/htslib) - for Ruby
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Ruby-htslib is the Ruby bindings to HTSlib, a C library for processing high throughput sequencing (HTS) data.
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It will provide APIs to read and write file formats such as SAM, BAM, VCF, and BCF.
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:apple: Feel free to fork it out if you can develop it!
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:bowtie:
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:bowtie: alpha stage.
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## Requirements
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* [Ruby](https://github.com/ruby/ruby) 2.7 or above.
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* [HTSlib](https://github.com/samtools/htslib)
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* Ubuntu : `apt install libhts-dev`
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* macOS : `brew install htslib`
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* Build from source code (see Development section)
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## Installation
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@@ -17,30 +28,53 @@
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gem install htslib
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```
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-
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If you have installed htslib with apt on Ubuntu or homebrew on Mac, [pkg-config](https://github.com/ruby-gnome/pkg-config)
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will automatically detect the location of the shared library.
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Alternatively, you can specify the directory of the shared library by setting the environment variable `HTSLIBDIR`.
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```sh
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export HTSLIBDIR="/your/path/to/htslib"
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export HTSLIBDIR="/your/path/to/htslib" # libhts.so
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```
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##
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-
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* [htslib](https://github.com/samtools/htslib)
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## Overview
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-
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### Low level API
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HTS::
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`HTS::LibHTS` provides native functions.
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```ruby
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require 'htslib'
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a = HTS::
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b = HTS::
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a = HTS::LibHTS.hts_open("a.bam", "r")
|
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b = HTS::LibHTS.hts_get_format(a)
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p b[:category]
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p b[:format]
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```
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-
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Note: Managed struct is not used in ruby-htslib. You may need to free the memory by yourself.
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### High level API (Plan)
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`Cram` `Bam` `Bcf` `Faidx` `Tabix`
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|
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A high-level API is under development. We will change and improve the API to make it better.
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|
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```ruby
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require 'htslib'
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bam = HTS::Bam.new("a.bam")
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|
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bam.each do |r|
|
68
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p name: r.qname,
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flag: r.flag,
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70
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start: r.start + 1,
|
71
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mpos: r.mate_pos + 1,
|
72
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mqual: r.mapping_quality,
|
73
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seq: r.sequence,
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74
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cigar: r.cigar.to_s,
|
75
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qual: r.base_qualities.map { |i| (i + 33).chr }.join
|
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end
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```
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## Documentation
|
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@@ -51,25 +85,42 @@ A high-level API based on [hts-python](https://github.com/quinlan-lab/hts-python
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To get started with development
|
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86
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|
53
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```sh
|
54
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git clone --
|
88
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+
git clone --recursive https://github.com/kojix2/ruby-htslib
|
55
89
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cd ruby-htslib
|
56
90
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bundle install
|
57
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-
bundle exec rake htslib:
|
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-
bundle exec rake
|
91
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bundle exec rake htslib:build
|
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bundle exec rake test
|
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```
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We plan to actively use the new features of Ruby. Since the number of users is small, backward compatibility is not important.
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On the other hand, we will consider compatibility with [Crystal](https://github.com/bio-crystal/htslib.cr) to some extent.
|
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|
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#### FFI Extensions
|
99
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|
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* [ffi-bitfield](https://github.com/kojix2/ffi-bitfield) : Extension of Ruby-FFI to support bitfields.
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|
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#### Automatic generation or automatic validation (Future plan)
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+ [c2ffi](https://github.com/rpav/c2ffi) is a tool to create JSON format metadata from C header files. It is planned to use c2ffi to automatically generate bindings or tests.
|
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## Contributing
|
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|
108
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Ruby-htslib is a library under development, so even small improvements like typofix are welcome! Please feel free to send us your pull requests.
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|
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* [Report bugs](https://github.com/kojix2/ruby-htslib/issues)
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* Fix bugs and [submit pull requests](https://github.com/kojix2/ruby-htslib/pulls)
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* Write, clarify, or fix documentation
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* Suggest or add new features
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* [financial contributions](https://github.com/sponsors/kojix2)
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## Links
|
69
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|
70
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* [samtools/hts-spec](https://github.com/samtools/hts-specs)
|
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-
* [
|
119
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* [bioruby](https://github.com/bioruby/bioruby)
|
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|
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## Funding support
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|
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This work was supported partially by [Ruby Association Grant 2020](https://www.ruby.or.jp/en/news/20201022).
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## License
|
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[MIT License](https://opensource.org/licenses/MIT).
|
data/lib/hts/bam/cigar.rb
CHANGED
@@ -7,22 +7,25 @@ module HTS
|
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7
7
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class Bam
|
8
8
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class Cigar
|
9
9
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include Enumerable
|
10
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-
OPS = 'MIDNSHP=XB'
|
11
10
|
|
12
|
-
def initialize(
|
13
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-
@
|
11
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+
def initialize(pointer, n_cigar)
|
12
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+
@pointer = pointer
|
14
13
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@n_cigar = n_cigar
|
15
14
|
end
|
16
15
|
|
16
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+
def to_ptr
|
17
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+
@pointer
|
18
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+
end
|
19
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+
|
17
20
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def to_s
|
18
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to_a.flatten.join
|
19
22
|
end
|
20
23
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21
24
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def each
|
22
25
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@n_cigar.times do |i|
|
23
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-
c = @
|
24
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-
yield [
|
25
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-
|
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+
c = @pointer[i].read_uint32
|
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+
yield [LibHTS.bam_cigar_oplen(c),
|
28
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+
LibHTS.bam_cigar_opchr(c)]
|
26
29
|
end
|
27
30
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end
|
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31
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end
|
data/lib/hts/bam/flag.rb
ADDED
@@ -0,0 +1,93 @@
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|
1
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# frozen_string_literal: true
|
2
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+
|
3
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# Based on hts-nim
|
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# https://github.com/brentp/hts-nim/blob/master/src/hts/bam/flag.nim
|
5
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+
|
6
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module HTS
|
7
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class Bam
|
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class Flag
|
9
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def initialize(flag_value)
|
10
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raise TypeError unless flag_value.is_a? Integer
|
11
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+
|
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@value = flag_value
|
13
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+
end
|
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attr_accessor :value
|
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|
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# BAM_FPAIRED = 1
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# BAM_FPROPER_PAIR = 2
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# BAM_FUNMAP = 4
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# BAM_FMUNMAP = 8
|
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# BAM_FREVERSE = 16
|
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# BAM_FMREVERSE = 32
|
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# BAM_FREAD1 = 64
|
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# BAM_FREAD2 = 128
|
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# BAM_FSECONDARY = 256
|
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# BAM_FQCFAIL = 512
|
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# BAM_FDUP = 1024
|
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# BAM_FSUPPLEMENTARY = 2048
|
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+
|
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# TODO: Enabling bitwise operations
|
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# hts-nim
|
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# proc `and`*(f: Flag, o: uint16): uint16 {. borrow, inline .}
|
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# proc `and`*(f: Flag, o: Flag): uint16 {. borrow, inline .}
|
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+
# proc `or`*(f: Flag, o: uint16): uint16 {. borrow .}
|
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+
# proc `or`*(o: uint16, f: Flag): uint16 {. borrow .}
|
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# proc `==`*(f: Flag, o: Flag): bool {. borrow, inline .}
|
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+
# proc `==`*(f: Flag, o: uint16): bool {. borrow, inline .}
|
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# proc `==`*(o: uint16, f: Flag): bool {. borrow, inline .}
|
39
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+
|
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def paired?
|
41
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+
has_flag? LibHTS::BAM_FPAIRED
|
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+
end
|
43
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+
|
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def proper_pair?
|
45
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+
has_flag? LibHTS::BAM_FPROPER_PAIR
|
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+
end
|
47
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+
|
48
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+
def unmapped?
|
49
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+
has_flag? LibHTS::BAM_FUNMAP
|
50
|
+
end
|
51
|
+
|
52
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+
def mate_unmapped?
|
53
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+
has_flag? LibHTS::BAM_FMUNMAP
|
54
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+
end
|
55
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+
|
56
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def reverse?
|
57
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has_flag? LibHTS::BAM_FREVERSE
|
58
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+
end
|
59
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+
|
60
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+
def mate_reverse?
|
61
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+
has_flag? LibHTS::BAM_FMREVERSE
|
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+
end
|
63
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+
|
64
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+
def read1?
|
65
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has_flag? LibHTS::BAM_FREAD1
|
66
|
+
end
|
67
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+
|
68
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+
def read2?
|
69
|
+
has_flag? LibHTS::BAM_FREAD2
|
70
|
+
end
|
71
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+
|
72
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+
def secondary?
|
73
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+
has_flag? LibHTS::BAM_FSECONDARY
|
74
|
+
end
|
75
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+
|
76
|
+
def qcfail?
|
77
|
+
has_flag? LibHTS::BAM_FQCFAIL
|
78
|
+
end
|
79
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+
|
80
|
+
def dup?
|
81
|
+
has_flag? LibHTS::BAM_FDUP
|
82
|
+
end
|
83
|
+
|
84
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+
def supplementary?
|
85
|
+
has_flag? LibHTS::BAM_FSUPPLEMENTARY
|
86
|
+
end
|
87
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+
|
88
|
+
def has_flag?(o)
|
89
|
+
@value[o] != 0
|
90
|
+
end
|
91
|
+
end
|
92
|
+
end
|
93
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+
end
|
data/lib/hts/bam/header.rb
CHANGED
@@ -6,21 +6,27 @@
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6
6
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module HTS
|
7
7
|
class Bam
|
8
8
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class Header
|
9
|
-
|
9
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+
def initialize(pointer)
|
10
|
+
@sam_hdr = pointer
|
11
|
+
end
|
12
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+
|
13
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+
def struct
|
14
|
+
@sam_hdr
|
15
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+
end
|
10
16
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|
11
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-
def
|
12
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-
@
|
17
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+
def to_ptr
|
18
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+
@sam_hdr.to_ptr
|
13
19
|
end
|
14
20
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|
15
21
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# FIXME: better name?
|
16
22
|
def seqs
|
17
|
-
Array.new(@
|
18
|
-
|
23
|
+
Array.new(@sam_hdr[:n_targets]) do |i|
|
24
|
+
LibHTS.sam_hdr_tid2name(@sam_hdr, i)
|
19
25
|
end
|
20
26
|
end
|
21
27
|
|
22
28
|
def text
|
23
|
-
|
29
|
+
LibHTS.sam_hdr_str(@sam_hdr)
|
24
30
|
end
|
25
31
|
end
|
26
32
|
end
|
@@ -0,0 +1,195 @@
|
|
1
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+
# frozen_string_literal: true
|
2
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+
|
3
|
+
# Based on hts-python
|
4
|
+
# https://github.com/quinlan-lab/hts-python
|
5
|
+
|
6
|
+
module HTS
|
7
|
+
class Bam
|
8
|
+
class Record
|
9
|
+
SEQ_NT16_STR = "=ACMGRSVTWYHKDBN"
|
10
|
+
|
11
|
+
def initialize(bam1_t, header)
|
12
|
+
@bam1 = bam1_t
|
13
|
+
@header = header
|
14
|
+
end
|
15
|
+
|
16
|
+
def struct
|
17
|
+
@bam1
|
18
|
+
end
|
19
|
+
|
20
|
+
def to_ptr
|
21
|
+
@bam1.to_ptr
|
22
|
+
end
|
23
|
+
|
24
|
+
attr_reader :header
|
25
|
+
|
26
|
+
# def initialize_copy
|
27
|
+
# super
|
28
|
+
# end
|
29
|
+
|
30
|
+
def self.rom_sam_str; end
|
31
|
+
|
32
|
+
def tags; end
|
33
|
+
|
34
|
+
# returns the query name.
|
35
|
+
def qname
|
36
|
+
LibHTS.bam_get_qname(@bam1).read_string
|
37
|
+
end
|
38
|
+
|
39
|
+
# Set (query) name.
|
40
|
+
# def qname=(name)
|
41
|
+
# raise 'Not Implemented'
|
42
|
+
# end
|
43
|
+
|
44
|
+
# returns the tid of the record or -1 if not mapped.
|
45
|
+
def tid
|
46
|
+
@bam1[:core][:tid]
|
47
|
+
end
|
48
|
+
|
49
|
+
# returns the tid of the mate or -1 if not mapped.
|
50
|
+
def mate_tid
|
51
|
+
@bam1[:core][:mtid]
|
52
|
+
end
|
53
|
+
|
54
|
+
# returns 0-based start position.
|
55
|
+
def start
|
56
|
+
@bam1[:core][:pos]
|
57
|
+
end
|
58
|
+
|
59
|
+
# returns end position of the read.
|
60
|
+
def stop
|
61
|
+
LibHTS.bam_endpos @bam1
|
62
|
+
end
|
63
|
+
|
64
|
+
# returns 0-based mate position
|
65
|
+
def mate_start
|
66
|
+
@bam1[:core][:mpos]
|
67
|
+
end
|
68
|
+
alias mate_pos mate_start
|
69
|
+
|
70
|
+
# returns the chromosome or '' if not mapped.
|
71
|
+
def chrom
|
72
|
+
tid = @bam1[:core][:tid]
|
73
|
+
return "" if tid == -1
|
74
|
+
|
75
|
+
LibHTS.sam_hdr_tid2name(@header, tid)
|
76
|
+
end
|
77
|
+
|
78
|
+
# returns the chromosome of the mate or '' if not mapped.
|
79
|
+
def mate_chrom
|
80
|
+
tid = @bam1[:core][:mtid]
|
81
|
+
return "" if tid == -1
|
82
|
+
|
83
|
+
LibHTS.sam_hdr_tid2name(@header, tid)
|
84
|
+
end
|
85
|
+
|
86
|
+
def strand
|
87
|
+
LibHTS.bam_is_rev(@bam1) ? "-" : "+"
|
88
|
+
end
|
89
|
+
|
90
|
+
# def start=(v)
|
91
|
+
# raise 'Not Implemented'
|
92
|
+
# end
|
93
|
+
|
94
|
+
# insert size
|
95
|
+
def isize
|
96
|
+
@bam1[:core][:isize]
|
97
|
+
end
|
98
|
+
|
99
|
+
# mapping quality
|
100
|
+
def mapping_quality
|
101
|
+
@bam1[:core][:qual]
|
102
|
+
end
|
103
|
+
|
104
|
+
# returns a `Cigar` object.
|
105
|
+
def cigar
|
106
|
+
Cigar.new(LibHTS.bam_get_cigar(@bam1), @bam1[:core][:n_cigar])
|
107
|
+
end
|
108
|
+
|
109
|
+
def qlen
|
110
|
+
LibHTS.bam_cigar2qlen(
|
111
|
+
@bam1[:core][:n_cigar],
|
112
|
+
LibHTS.bam_get_cigar(@bam1)
|
113
|
+
)
|
114
|
+
end
|
115
|
+
|
116
|
+
def rlen
|
117
|
+
LibHTS.bam_cigar2rlen(
|
118
|
+
@bam1[:core][:n_cigar],
|
119
|
+
LibHTS.bam_get_cigar(@bam1)
|
120
|
+
)
|
121
|
+
end
|
122
|
+
|
123
|
+
# return the read sequence
|
124
|
+
def sequence
|
125
|
+
r = LibHTS.bam_get_seq(@bam1)
|
126
|
+
seq = String.new
|
127
|
+
(@bam1[:core][:l_qseq]).times do |i|
|
128
|
+
seq << SEQ_NT16_STR[LibHTS.bam_seqi(r, i)]
|
129
|
+
end
|
130
|
+
seq
|
131
|
+
end
|
132
|
+
|
133
|
+
# return only the base of the requested index "i" of the query sequence.
|
134
|
+
def base_at(n)
|
135
|
+
n += @bam1[:core][:l_qseq] if n < 0
|
136
|
+
return "." if (n >= @bam1[:core][:l_qseq]) || (n < 0) # eg. base_at(-1000)
|
137
|
+
|
138
|
+
r = LibHTS.bam_get_seq(@bam1)
|
139
|
+
SEQ_NT16_STR[LibHTS.bam_seqi(r, n)]
|
140
|
+
end
|
141
|
+
|
142
|
+
# return the base qualities
|
143
|
+
def base_qualities
|
144
|
+
q_ptr = LibHTS.bam_get_qual(@bam1)
|
145
|
+
q_ptr.read_array_of_uint8(@bam1[:core][:l_qseq])
|
146
|
+
end
|
147
|
+
|
148
|
+
# return only the base quality of the requested index "i" of the query sequence.
|
149
|
+
def base_quality_at(n)
|
150
|
+
n += @bam1[:core][:l_qseq] if n < 0
|
151
|
+
return 0 if (n >= @bam1[:core][:l_qseq]) || (n < 0) # eg. base_quality_at(-1000)
|
152
|
+
|
153
|
+
q_ptr = LibHTS.bam_get_qual(@bam1)
|
154
|
+
q_ptr.get_uint8(n)
|
155
|
+
end
|
156
|
+
|
157
|
+
def flag_str
|
158
|
+
LibHTS.bam_flag2str(@bam1[:core][:flag])
|
159
|
+
end
|
160
|
+
|
161
|
+
# returns a `Flag` object.
|
162
|
+
def flag
|
163
|
+
Flag.new(@bam1[:core][:flag])
|
164
|
+
end
|
165
|
+
|
166
|
+
def tag(str)
|
167
|
+
aux = LibHTS.bam_aux_get(@bam1, str)
|
168
|
+
return nil if aux.null?
|
169
|
+
|
170
|
+
t = aux.read_string(1)
|
171
|
+
case t
|
172
|
+
when "i", "I", "c", "C", "s", "S"
|
173
|
+
LibHTS.bam_aux2i(aux)
|
174
|
+
when "f", "d"
|
175
|
+
LibHTS.bam_aux2f(aux)
|
176
|
+
when "Z", "H"
|
177
|
+
LibHTS.bam_aux2Z(aux)
|
178
|
+
when "A"
|
179
|
+
LibHTS.bam_aux2A(aux)
|
180
|
+
end
|
181
|
+
end
|
182
|
+
|
183
|
+
def to_s
|
184
|
+
kstr = LibHTS::KString.new
|
185
|
+
raise "Failed to format bam record" if LibHTS.sam_format1(@header.struct, @bam1, kstr) == -1
|
186
|
+
|
187
|
+
kstr[:s]
|
188
|
+
end
|
189
|
+
|
190
|
+
# TODO:
|
191
|
+
# def eql?
|
192
|
+
# def hash
|
193
|
+
end
|
194
|
+
end
|
195
|
+
end
|
data/lib/hts/bam.rb
CHANGED
@@ -3,76 +3,111 @@
|
|
3
3
|
# Based on hts-python
|
4
4
|
# https://github.com/quinlan-lab/hts-python
|
5
5
|
|
6
|
-
require_relative
|
7
|
-
require_relative
|
8
|
-
require_relative
|
6
|
+
require_relative "bam/header"
|
7
|
+
require_relative "bam/cigar"
|
8
|
+
require_relative "bam/flag"
|
9
|
+
require_relative "bam/record"
|
9
10
|
|
10
11
|
module HTS
|
11
12
|
class Bam
|
12
13
|
include Enumerable
|
13
|
-
attr_reader :fname, :mode, :header, :htf
|
14
14
|
|
15
|
-
|
16
|
-
|
17
|
-
|
15
|
+
attr_reader :file_path, :mode, :header
|
16
|
+
# HtfFile is FFI::BitStruct
|
17
|
+
attr_reader :htf_file
|
18
18
|
|
19
|
-
|
20
|
-
|
19
|
+
class << self
|
20
|
+
alias open new
|
21
|
+
end
|
21
22
|
|
22
|
-
|
23
|
-
|
24
|
-
|
25
|
-
|
26
|
-
|
27
|
-
|
28
|
-
|
29
|
-
@header = Bam::Header.new(FFI.sam_hdr_read(@htf))
|
30
|
-
@b = FFI.bam_init1
|
23
|
+
def initialize(file_path, mode = "r", create_index: nil)
|
24
|
+
file_path = File.expand_path(file_path)
|
25
|
+
|
26
|
+
unless File.exist?(file_path)
|
27
|
+
message = "No such SAM/BAM file - #{file_path}"
|
28
|
+
raise message
|
29
|
+
end
|
31
30
|
|
31
|
+
@file_path = file_path
|
32
|
+
@mode = mode
|
33
|
+
@htf_file = LibHTS.hts_open(file_path, mode)
|
34
|
+
@header = Bam::Header.new(LibHTS.sam_hdr_read(htf_file))
|
35
|
+
|
36
|
+
# read
|
37
|
+
if mode[0] == "r"
|
38
|
+
# load index
|
39
|
+
@idx = LibHTS.sam_index_load(htf_file, file_path)
|
40
|
+
# create index
|
41
|
+
if create_index || (@idx.null? && create_index.nil?)
|
42
|
+
warn "Create index for #{file_path}"
|
43
|
+
LibHTS.sam_index_build(file_path, -1)
|
44
|
+
@idx = LibHTS.sam_index_load(htf_file, file_path)
|
45
|
+
end
|
32
46
|
else
|
33
|
-
# FIXME
|
34
|
-
raise
|
47
|
+
# FIXME: implement
|
48
|
+
raise "not implemented yet."
|
49
|
+
end
|
35
50
|
|
51
|
+
# IO like API
|
52
|
+
if block_given?
|
53
|
+
begin
|
54
|
+
yield self
|
55
|
+
ensure
|
56
|
+
close
|
57
|
+
end
|
36
58
|
end
|
37
59
|
end
|
38
60
|
|
39
|
-
def
|
61
|
+
def struct
|
62
|
+
htf_file
|
63
|
+
end
|
64
|
+
|
65
|
+
def to_ptr
|
66
|
+
htf_file.to_ptr
|
67
|
+
end
|
40
68
|
|
41
69
|
def write(alns)
|
42
70
|
alns.each do
|
43
|
-
|
71
|
+
LibHTS.sam_write1(htf_file, header, alns.b) > 0 || raise
|
44
72
|
end
|
45
73
|
end
|
46
74
|
|
47
75
|
# Close the current file.
|
48
76
|
def close
|
49
|
-
|
77
|
+
LibHTS.hts_close(htf_file)
|
50
78
|
end
|
51
79
|
|
52
80
|
# Flush the current file.
|
53
81
|
def flush
|
54
|
-
|
55
|
-
# FFI.bgzf_flush(@htf.fp.bgzf)
|
82
|
+
# LibHTS.bgzf_flush(@htf_file.fp.bgzf)
|
56
83
|
end
|
57
84
|
|
58
|
-
def each
|
85
|
+
def each
|
59
86
|
# Each does not always start at the beginning of the file.
|
60
87
|
# This is the common behavior of IO objects in Ruby.
|
61
88
|
# This may change in the future.
|
62
|
-
|
89
|
+
return to_enum(__method__) unless block_given?
|
90
|
+
|
91
|
+
while LibHTS.sam_read1(htf_file, header, bam1 = LibHTS.bam_init1) > 0
|
92
|
+
record = Record.new(bam1, header)
|
93
|
+
yield record
|
94
|
+
end
|
95
|
+
self
|
63
96
|
end
|
64
97
|
|
65
98
|
# query [WIP]
|
66
99
|
def query(region)
|
67
|
-
qiter =
|
100
|
+
qiter = LibHTS.sam_itr_querys(@idx, header, region)
|
68
101
|
begin
|
69
|
-
|
102
|
+
bam1 = LibHTS.bam_init1
|
103
|
+
slen = LibHTS.sam_itr_next(htf_file, qiter, bam1)
|
70
104
|
while slen > 0
|
71
|
-
yield
|
72
|
-
|
105
|
+
yield Record.new(bam1, header)
|
106
|
+
bam1 = LibHTS.bam_init1
|
107
|
+
slen = LibHTS.sam_itr_next(htf_file, qiter, bam1)
|
73
108
|
end
|
74
109
|
ensure
|
75
|
-
|
110
|
+
LibHTS.hts_itr_destroy(qiter)
|
76
111
|
end
|
77
112
|
end
|
78
113
|
end
|