bio-ucsc-api 0.5.3 → 0.6.0
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- data/ChangeLog.md +2 -0
- data/Gemfile +14 -13
- data/Gemfile.lock +50 -37
- data/LICENSE.txt +20 -0
- data/README.md +58 -37
- data/Rakefile +1 -1
- data/VERSION +1 -1
- data/bio-ucsc-api.gemspec +33 -29
- data/lib/#bio-ucsc.rb# +209 -0
- data/lib/bio-ucsc.rb +6 -2
- data/samples/bed2refseq.rb +4 -5
- data/samples/hg19-2bit-retrieve.rb +2 -3
- data/samples/num-gene-exon.rb +4 -3
- metadata +52 -40
- data/COPYING +0 -58
- data/COPYING.ja +0 -53
data/ChangeLog.md
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#Change Log
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* **NEW** (v0.6.0): Support ActiveRecord v4.0 (a part of Ruby on Ralils v4.0). Other related library dependencies are also updated. To Supress deprecation warnings for using dynamic finders such as 'find_by_name_and_chrom', use `ActiveSupport::Deprecation.silenced = true`. Because ActiveRecord v4.0 does not support Ruby v1.8.7 and earlier, Ruby UCSC API no longer supports these older Rubies.
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* **UPDATE** (v0.6.0): Ruby UCSC API is now licensed under the MIT License. See also the LICENSE.txt file.
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* **BUG-FIX** (v 0.5.3): Support ActiveRecord/ActiveSupport version 3 only.
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* **UPDATE** (v 0.5.3): The Rat Rn5 database is supported.
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* **UPDATE** (v 0.5.3): Bug-fix and update to pass all specs on Ruby 2.0.0-p0.
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data/Gemfile
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source "http://rubygems.org"
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# Add dependencies required to use your gem here.
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gem "
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gem "
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gem "
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gem "
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gem "
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gem "activerecord", "~> 4.0.2"
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gem "activesupport", "~> 4.0.2"
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gem "mysql", "~> 2.9.1"
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gem "safe_attributes", "~> 1.0.10"
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gem "activerecord-deprecated_finders", "~> 1.0.3"
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gem "bio-genomic-interval", ">= 0.1.2"
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# Add dependencies to develop your gem here.
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# Include everything needed to run rake, tests, features, etc.
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group :development do
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gem "rake",
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gem "rspec",
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gem "bundler", "~> 1.3.5"
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gem "jeweler", "~> 1.8.
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gem "bio",
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gem "rake", "~> 10.1.0"
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gem "rspec", "~> 2.14.1"
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gem "bundler", "~> 1.3.5"
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gem "jeweler", "~> 1.8.8"
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gem "bio", ">= 1.4.3"
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gem "rdoc", ">= 4.0.1"
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# gem "simplecov", :require => false, :group => :test
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end
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data/Gemfile.lock
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GEM
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remote: http://rubygems.org/
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specs:
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activemodel (
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activesupport (=
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builder (~>
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activemodel (4.0.2)
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activesupport (= 4.0.2)
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builder (~> 3.1.0)
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activerecord (4.0.2)
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activemodel (= 4.0.2)
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activerecord-deprecated_finders (~> 1.0.2)
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activesupport (= 4.0.2)
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arel (~> 4.0.0)
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activerecord-deprecated_finders (1.0.3)
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activesupport (4.0.2)
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bio (1.4.3.0001)
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jeweler (1.8.
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i18n (0.6.9)
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jeweler (1.8.8)
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builder
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minitest (4.7.5)
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multi_xml (0.5.5)
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rspec-expectations (~> 2.14.0)
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rspec-core (2.14.7)
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rspec-expectations (2.14.4)
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diff-lcs (>= 1.1.3, < 2.0)
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rspec-mocks (2.14.4)
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safe_attributes (1.0.10)
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activerecord (>= 3.0.0)
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thread_safe (0.1.3)
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atomic
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thread_safe (0.1.3-java)
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atomic
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tzinfo (0.3.38)
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PLATFORMS
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java
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ruby
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DEPENDENCIES
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activerecord (~>
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activerecord (~> 4.0.2)
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activesupport (~> 4.0.2)
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bio (>= 1.4.3)
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bio-genomic-interval (>= 0.1.2)
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bundler (~> 1.3.5)
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rake (~> 10.1.0)
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rdoc (>=
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safe_attributes (
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safe_attributes (~> 1.0.10)
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data/LICENSE.txt
ADDED
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Copyright (c) 2011-2013 MISHIMA, Hiroyuki
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Permission is hereby granted, free of charge, to any person obtaining
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a copy of this software and associated documentation files (the
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"Software"), to deal in the Software without restriction, including
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without limitation the rights to use, copy, modify, merge, publish,
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distribute, sublicense, and/or sell copies of the Software, and to
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permit persons to whom the Software is furnished to do so, subject to
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the following conditions:
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The above copyright notice and this permission notice shall be
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included in all copies or substantial portions of the Software.
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THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
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EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF
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MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
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NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE
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LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION
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OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION
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WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.
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data/README.md
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# bio-ucsc-api version 0.
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# bio-ucsc-api version 0.6.0
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**The Ruby UCSC API**: accessing the UCSC Genome Database using Ruby.
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* Automatic conversion of "1-based full-closed intervals" to internal "0-based left-closed right-open intervals" (see also bioruby-genomic-interval)
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* Supporting non-official full/partial mirror MySql hosts (e.g. local servers)
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* Using Rspec for the testing framework
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* Written in pure Ruby and supporting multiple Ruby interpreter implementations including Ruby1.
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* Written in pure Ruby and supporting multiple Ruby interpreter implementations including Ruby1.9, Ruby2.0and JRuby1.6
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* Designed as a BioRuby plugin
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* Current version does not support table-linked bigWIG/bigBED/BAM files.
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* Ruby Version 2.0.0 or later
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* Ruby version 1.9.2 or later
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* Ruby version 1.8.7 or later
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* JRuby version 1.6.3 or later - Appropiate Java heap size may have to be specified to invoke JRuby, especially when you use Bio::Ucsc::File::Twobit. Try "jruby -J-Xmx3g your_script.rb" to keep 3G byte heap.
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* Ruby version 1.8.7 or earlier are no longer supported by UCSC API v0.6.0 and later because Ruby on Rails and ActiveRecord version 4.0 do not support these old Rubies.
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Major rubygem dependencies:
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* active_record version **4.0** - http://api.rubyonrails.org/classes/ActiveRecord/Base.html
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* safe_attributes - https://github.com/bjones/safe_attributes
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* activerecord-deprecated_finders - http://github.com/rails/activerecord-deprecated_finders -- supporting AR4.0 deprecated dynamic finders shch as 'find_all_by_name'. To suppress deprecation warnings, you have to add `ActiveSupport::Deprecation.silenced = true`.
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* bioruby-genomic-interval - https://github.com/misshie/bioruby-genomic-interval
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* mysql (MySQL/Ruby MySQL API module) - http://www.tmtm.org/mysql/ruby/README.html
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* UCSCBin library - https://github.com/misshie/UCSCBin
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# Change Log
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-
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* **NEW** (v0.6.0): Support ActiveRecord v4.0 (a part of Ruby on Ralils v4.0). Other related library dependencies are also updated. To Supress deprecation warnings for using dynamic finders such as 'find_by_name_and_chrom', use `ActiveSupport::Deprecation.silenced = true`. Because ActiveRecord v4.0 does not support Ruby v1.8.7 and earlier, Ruby UCSC API no longer supports these older Rubies.
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* **UPDATE** (v0.6.0): Ruby UCSC API is now licensed under the MIT License. See also the LICENSE.txt file.
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See 'ChangeLog.md' for older changes.
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# How to Use
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## Basics
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* A database of a genome assembly is represented as a module in the `Bio::Ucsc module
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* Before using a database, establish a connection to the database. For example, `Bio::Ucsc::Hg19
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* A table in a database is represented as a class in the database module. For example, the
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* Queries to a field (column) in a table are represented by class methods of the table class. For example, finding the first record (row) of the
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* A database of a genome assembly is represented as a module in the `Bio::Ucsc` module. For example, the human hg19 database is referred by `Bio::Ucsc::Hg19`.
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* Before using a database, establish a connection to the database. For example, `Bio::Ucsc::Hg19.connect`.
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* A table in a database is represented as a class in the database module. For example, the snp138 table in the hg19 database is referred by `Bio::Ucsc::Hg19::Snp138`.
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* Queries to a field (column) in a table are represented by class methods of the table class. For example, finding the first record (row) of the snp138 table in the hg19 database is `Bio::Ucsc::Hg19::Snp138.first`.
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* Queries using genomic intervals are supported by the named scope ".with_intervals" and ".with_intervals_excl (omitting pertially included annotations)" method of the table class. These methods accept a genomic interval string like `chr1:1233-5678`. If a table to query has the "bin" column, the bin index system is automatically used to speed-up the query.
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* Fields in a retrieved record can be acccessed by using instance methods of a record object. For example, the name field of a table record stored in the result" variable is `result.name`.
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@@ -87,49 +92,66 @@ At first, you have to declare the API and establish the connection to a database
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```ruby
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require 'bio-ucsc'
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-
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-
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DB = Bio::Ucsc::Hg19
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DB.connect
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# Suppressing deprecation warnings for using dynamic finders such as 'find_by_name_and_chrom'.
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# These syles are deprecated in ActiveRecord 4.0.
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ActiveSupport::Deprecation.silenced = true
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```
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Table search using genomic intervals:
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```ruby
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-
Ucsc::Hg19
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DB = Bio::Ucsc::Hg19
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DB.connect
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DB::Snp138.with_interval("chr1:1-11,000").find(:all).each do |e|
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i = GenomicInterval.zero_based(e.chrom, e.chromStart, e.chromEnd)
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puts "#{i.chrom}\t#{i.chr_start}\t#{e.name}\t#{e[:class]}" # "e.class" does not work
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end
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gi = "chr17:7,579,614-7,579,700"
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puts
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puts DB::Snp138.with_interval(gi).find(:all)
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puts
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puts DB::Snp138.with_interval_excl(gi).find(:all)
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-
relation =
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relation = DB::Snp138.with_interval(gi).select(:name)
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puts relation.to_sql
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# => SELECT name FROM `
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-
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-
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-
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# => SELECT name FROM `snp138`
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# WHERE (chrom = 'chr17' AND bin in (642,80,9,1,0)
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# AND ((chromStart BETWEEN 7579613 AND 7579700) AND
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# (chromEnd BETWEEN 7579613 AND 7579700)))
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puts relation.find_all_by_class_and_strand("in-del", "+").size # => 1
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-
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# Rails4 style
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puts DB::Snp138.where(name: "rs56289060").first
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+
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# Old style
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ActiveSupport::Deprecation.silenced = true # Suppress warnings
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puts DB::Snp138.find_all_by_name("rs56289060").first
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```
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|
Sometimes, queries using raw SQLs provide elegant solutions.
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|
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|
```ruby
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+
DB = Bio::Ucsc::Hg19
|
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|
+
DB.connect
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|
+
|
121
141
|
sql << 'SQL'
|
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|
SELECT name,chrom,chromStart,chromEnd,observed
|
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|
-
FROM
|
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|
+
FROM snp138
|
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|
WHERE name="rs56289060"
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|
SQL
|
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|
-
puts
|
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|
+
puts DB::Snp138.find_by_sql(sql)
|
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|
```
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148
|
For gene prediction (genePred) tables, such as RefSeq, EndGene, and WgEncodeGencodeBasicV12, Ruby UCSC API automatically implements `#exon`, `#introns`, `#cdss` (or an alias `#cdses`) methods. Exons, introns, and CDSes are accessible as Array objects of `Bio::GenomicInterval`.
|
129
149
|
|
130
150
|
```ruby
|
131
|
-
Bio::Ucsc::Hg19
|
132
|
-
|
151
|
+
DB = Bio::Ucsc::Hg19
|
152
|
+
DB.connect
|
153
|
+
|
154
|
+
r = DB::RefGene.with_interval("chr1:1,000,000-1,100,000").first
|
133
155
|
puts "gene strand = #{r.strand}"
|
134
156
|
r.exons.each{|x|puts "[#{x.chr_start}, #{x.chr_end}]"}
|
135
157
|
r.cdss.each{|x|puts "[#{x.chr_start}, #{x.chr_end}]"}
|
@@ -139,22 +161,22 @@ For gene prediction (genePred) tables, such as RefSeq, EndGene, and WgEncodeGenc
|
|
139
161
|
retrieve reference sequence from a locally-stored 2bit file. The "hg19.2bit" file can be downloaded from http://hgdownload.cse.ucsc.edu/goldenPath/hg19/bigZips/hg19.2bit
|
140
162
|
|
141
163
|
```ruby
|
142
|
-
hg19ref = Ucsc::File::Twobit.load("hg19.2bit")
|
164
|
+
hg19ref = Bio::Ucsc::File::Twobit.load("hg19.2bit")
|
143
165
|
puts hg19ref.find_by_interval("chr1:9,500-10,999")
|
144
166
|
|
145
167
|
# another way to access a twobit file
|
146
|
-
puts Ucsc::File::Twobit.open("hg19.2bit"){|tb|tb.find_by_interval("chr1:9,500-10,999")}
|
168
|
+
puts Bio::Ucsc::File::Twobit.open("hg19.2bit"){|tb|tb.find_by_interval("chr1:9,500-10,999")}
|
147
169
|
```
|
148
170
|
|
149
171
|
Connetcting to non-official or local full/partial mirror MySQL servers
|
150
172
|
|
151
173
|
```ruby
|
152
|
-
Ucsc::Hg18.connect( :db_host => 'localhost',
|
153
|
-
|
154
|
-
|
174
|
+
Bio::Ucsc::Hg18.connect( :db_host => 'localhost',
|
175
|
+
:db_username => 'genome',
|
176
|
+
:db_password => '' )
|
155
177
|
|
156
|
-
Ucsc::Hg18.default # reset to connect UCSC's public MySQL sever
|
157
|
-
Ucsc::Hg18.connect
|
178
|
+
Bio::Ucsc::Hg18.default # reset to connect UCSC's public MySQL sever
|
179
|
+
Bio::Ucsc::Hg18.connect
|
158
180
|
```
|
159
181
|
|
160
182
|
And see also sample scripts in the samples directory.
|
@@ -226,13 +248,12 @@ Bio::Ucsc::Hg19.connect
|
|
226
248
|
Bio::Ucsc::Hg18.connect
|
227
249
|
joiner = Bio::Ucsc::Schema::Joiner.load
|
228
250
|
joiner.variables["gbd"] = ["hg19", "hg18"]
|
229
|
-
joiner.define_association(Bio::Ucsc::Hg19::
|
230
|
-
# "first" is required because the
|
231
|
-
puts Bio::Ucsc::Hg19::
|
251
|
+
joiner.define_association(Bio::Ucsc::Hg19::Snp138)
|
252
|
+
# "first" is required because the snp138Seq method always returns an array.
|
253
|
+
puts Bio::Ucsc::Hg19::Snp138.find_by_name("rs242").snp138Seq.first.file_offset
|
232
254
|
```
|
233
255
|
|
234
256
|
# Copyright
|
235
257
|
**Copyright**: (c) 2011-2013 MISHIMA, Hiroyuki (hmishima at nagasaki-u.ac.jp / Twitter: @mishima_eng (in English) and @mishimahryk (in Japanese)
|
236
258
|
**Copyright**: (c) 2010 Jan Aerts
|
237
|
-
**License**:
|
238
|
-
|
259
|
+
**License**: The MIT license. See LICENSE.txt for further details..
|
data/Rakefile
CHANGED
@@ -17,7 +17,7 @@ Jeweler::Tasks.new do |gem|
|
|
17
17
|
# see http://docs.rubygems.org/read/chapter/20 for more options
|
18
18
|
gem.name = "bio-ucsc-api"
|
19
19
|
gem.homepage = "http://github.com/misshie/bioruby-ucsc-api"
|
20
|
-
gem.license = "
|
20
|
+
gem.license = "The MIT License"
|
21
21
|
gem.summary = %Q{The Ruby UCSC API: accessing the UCSC Genome Database using Ruby}
|
22
22
|
gem.description = %Q{Ruby UCSC API: accessing the UCSC Genome Database using Ruby}
|
23
23
|
gem.email = "missy@be.to"
|
data/VERSION
CHANGED
@@ -1 +1 @@
|
|
1
|
-
0.
|
1
|
+
0.6.0
|
data/bio-ucsc-api.gemspec
CHANGED
@@ -5,27 +5,28 @@
|
|
5
5
|
|
6
6
|
Gem::Specification.new do |s|
|
7
7
|
s.name = "bio-ucsc-api"
|
8
|
-
s.version = "0.
|
8
|
+
s.version = "0.6.0"
|
9
9
|
|
10
10
|
s.required_rubygems_version = Gem::Requirement.new(">= 0") if s.respond_to? :required_rubygems_version=
|
11
11
|
s.authors = ["Hiroyuki Mishima", "Jan Aerts"]
|
12
|
-
s.date = "2013-
|
12
|
+
s.date = "2013-12-11"
|
13
13
|
s.description = "Ruby UCSC API: accessing the UCSC Genome Database using Ruby"
|
14
14
|
s.email = "missy@be.to"
|
15
15
|
s.extra_rdoc_files = [
|
16
16
|
"ChangeLog.md",
|
17
|
+
"LICENSE.txt",
|
17
18
|
"README.md"
|
18
19
|
]
|
19
20
|
s.files = [
|
20
|
-
"COPYING",
|
21
|
-
"COPYING.ja",
|
22
21
|
"ChangeLog.md",
|
23
22
|
"Gemfile",
|
24
23
|
"Gemfile.lock",
|
24
|
+
"LICENSE.txt",
|
25
25
|
"README.md",
|
26
26
|
"Rakefile",
|
27
27
|
"VERSION",
|
28
28
|
"bio-ucsc-api.gemspec",
|
29
|
+
"lib/#bio-ucsc.rb#",
|
29
30
|
"lib/bio-ucsc-api.rb",
|
30
31
|
"lib/bio-ucsc.rb",
|
31
32
|
"lib/bio-ucsc/ailmel1.rb",
|
@@ -433,7 +434,7 @@ Gem::Specification.new do |s|
|
|
433
434
|
"samples/symbol2summary.rb"
|
434
435
|
]
|
435
436
|
s.homepage = "http://github.com/misshie/bioruby-ucsc-api"
|
436
|
-
s.licenses = ["
|
437
|
+
s.licenses = ["The MIT License"]
|
437
438
|
s.require_paths = ["lib"]
|
438
439
|
s.rubygems_version = "1.8.11"
|
439
440
|
s.summary = "The Ruby UCSC API: accessing the UCSC Genome Database using Ruby"
|
@@ -442,42 +443,45 @@ Gem::Specification.new do |s|
|
|
442
443
|
s.specification_version = 3
|
443
444
|
|
444
445
|
if Gem::Version.new(Gem::VERSION) >= Gem::Version.new('1.2.0') then
|
445
|
-
s.add_runtime_dependency(%q<activerecord>, ["~>
|
446
|
-
s.add_runtime_dependency(%q<activesupport>, ["~>
|
447
|
-
s.add_runtime_dependency(%q<mysql>, ["~> 2.
|
446
|
+
s.add_runtime_dependency(%q<activerecord>, ["~> 4.0.2"])
|
447
|
+
s.add_runtime_dependency(%q<activesupport>, ["~> 4.0.2"])
|
448
|
+
s.add_runtime_dependency(%q<mysql>, ["~> 2.9.1"])
|
449
|
+
s.add_runtime_dependency(%q<safe_attributes>, ["~> 1.0.10"])
|
450
|
+
s.add_runtime_dependency(%q<activerecord-deprecated_finders>, ["~> 1.0.3"])
|
448
451
|
s.add_runtime_dependency(%q<bio-genomic-interval>, [">= 0.1.2"])
|
449
|
-
s.add_runtime_dependency(%q<safe_attributes>, [">= 1.0.8"])
|
450
452
|
s.add_development_dependency(%q<rake>, ["~> 10.1.0"])
|
451
|
-
s.add_development_dependency(%q<rspec>, ["~> 2.
|
453
|
+
s.add_development_dependency(%q<rspec>, ["~> 2.14.1"])
|
452
454
|
s.add_development_dependency(%q<bundler>, ["~> 1.3.5"])
|
453
|
-
s.add_development_dependency(%q<jeweler>, ["~> 1.8.
|
454
|
-
s.add_development_dependency(%q<bio>, [">= 1.4.
|
455
|
-
s.add_development_dependency(%q<rdoc>, [">=
|
455
|
+
s.add_development_dependency(%q<jeweler>, ["~> 1.8.8"])
|
456
|
+
s.add_development_dependency(%q<bio>, [">= 1.4.3"])
|
457
|
+
s.add_development_dependency(%q<rdoc>, [">= 4.0.1"])
|
456
458
|
else
|
457
|
-
s.add_dependency(%q<activerecord>, ["~>
|
458
|
-
s.add_dependency(%q<activesupport>, ["~>
|
459
|
-
s.add_dependency(%q<mysql>, ["~> 2.
|
459
|
+
s.add_dependency(%q<activerecord>, ["~> 4.0.2"])
|
460
|
+
s.add_dependency(%q<activesupport>, ["~> 4.0.2"])
|
461
|
+
s.add_dependency(%q<mysql>, ["~> 2.9.1"])
|
462
|
+
s.add_dependency(%q<safe_attributes>, ["~> 1.0.10"])
|
463
|
+
s.add_dependency(%q<activerecord-deprecated_finders>, ["~> 1.0.3"])
|
460
464
|
s.add_dependency(%q<bio-genomic-interval>, [">= 0.1.2"])
|
461
|
-
s.add_dependency(%q<safe_attributes>, [">= 1.0.8"])
|
462
465
|
s.add_dependency(%q<rake>, ["~> 10.1.0"])
|
463
|
-
s.add_dependency(%q<rspec>, ["~> 2.
|
466
|
+
s.add_dependency(%q<rspec>, ["~> 2.14.1"])
|
464
467
|
s.add_dependency(%q<bundler>, ["~> 1.3.5"])
|
465
|
-
s.add_dependency(%q<jeweler>, ["~> 1.8.
|
466
|
-
s.add_dependency(%q<bio>, [">= 1.4.
|
467
|
-
s.add_dependency(%q<rdoc>, [">=
|
468
|
+
s.add_dependency(%q<jeweler>, ["~> 1.8.8"])
|
469
|
+
s.add_dependency(%q<bio>, [">= 1.4.3"])
|
470
|
+
s.add_dependency(%q<rdoc>, [">= 4.0.1"])
|
468
471
|
end
|
469
472
|
else
|
470
|
-
s.add_dependency(%q<activerecord>, ["~>
|
471
|
-
s.add_dependency(%q<activesupport>, ["~>
|
472
|
-
s.add_dependency(%q<mysql>, ["~> 2.
|
473
|
+
s.add_dependency(%q<activerecord>, ["~> 4.0.2"])
|
474
|
+
s.add_dependency(%q<activesupport>, ["~> 4.0.2"])
|
475
|
+
s.add_dependency(%q<mysql>, ["~> 2.9.1"])
|
476
|
+
s.add_dependency(%q<safe_attributes>, ["~> 1.0.10"])
|
477
|
+
s.add_dependency(%q<activerecord-deprecated_finders>, ["~> 1.0.3"])
|
473
478
|
s.add_dependency(%q<bio-genomic-interval>, [">= 0.1.2"])
|
474
|
-
s.add_dependency(%q<safe_attributes>, [">= 1.0.8"])
|
475
479
|
s.add_dependency(%q<rake>, ["~> 10.1.0"])
|
476
|
-
s.add_dependency(%q<rspec>, ["~> 2.
|
480
|
+
s.add_dependency(%q<rspec>, ["~> 2.14.1"])
|
477
481
|
s.add_dependency(%q<bundler>, ["~> 1.3.5"])
|
478
|
-
s.add_dependency(%q<jeweler>, ["~> 1.8.
|
479
|
-
s.add_dependency(%q<bio>, [">= 1.4.
|
480
|
-
s.add_dependency(%q<rdoc>, [">=
|
482
|
+
s.add_dependency(%q<jeweler>, ["~> 1.8.8"])
|
483
|
+
s.add_dependency(%q<bio>, [">= 1.4.3"])
|
484
|
+
s.add_dependency(%q<rdoc>, [">= 4.0.1"])
|
481
485
|
end
|
482
486
|
end
|
483
487
|
|
data/lib/#bio-ucsc.rb#
ADDED
@@ -0,0 +1,209 @@
|
|
1
|
+
#
|
2
|
+
# = bio-ucsc.rb
|
3
|
+
# Copyright:: Copyright (C) 2011-2013
|
4
|
+
# MISHIMA, Hiroyuki <missy at be.to / hmishima at nagasaki-u.ac.jp>
|
5
|
+
# License:: Ruby licence (Ryby's / GPLv2 dual)
|
6
|
+
|
7
|
+
base = "#{::File.dirname(__FILE__)}/bio-ucsc"
|
8
|
+
|
9
|
+
require 'rubygems'
|
10
|
+
gem 'activerecord', "~> 4.0.2"
|
11
|
+
require "active_record"
|
12
|
+
gem "safe_attributes"
|
13
|
+
require "safe_attributes"
|
14
|
+
gem "activerecord-deprecated_finders"
|
15
|
+
require "active_record/deprecated_finders"
|
16
|
+
#ActiveSupport::Deprecation.silenced = true
|
17
|
+
|
18
|
+
require "#{base}/ucsc_bin"
|
19
|
+
require "#{base}/genomic-interval-bin"
|
20
|
+
require "#{base}/gi.rb"
|
21
|
+
|
22
|
+
module Bio
|
23
|
+
module Ucsc
|
24
|
+
VERSION = "0.6.0"
|
25
|
+
base = "#{::File.dirname(__FILE__)}/bio-ucsc"
|
26
|
+
|
27
|
+
# mammmals #####################################
|
28
|
+
# human genome assemblies
|
29
|
+
autoload :Hg19, "#{base}/hg19"
|
30
|
+
autoload :Hg18, "#{base}/hg18"
|
31
|
+
|
32
|
+
# chimp genome assemblies
|
33
|
+
autoload :PanTro3, "#{base}/pantro3"
|
34
|
+
|
35
|
+
# orangutan genome assemblies
|
36
|
+
autoload :PonAbe2, "#{base}/ponabe2"
|
37
|
+
|
38
|
+
# rhesus macaque genome assemblies
|
39
|
+
autoload :RheMac2, "#{base}/rhemac2"
|
40
|
+
|
41
|
+
# marmoset genome assemblies
|
42
|
+
autoload :CalJac3, "#{base}/caljac3"
|
43
|
+
|
44
|
+
# mouse genome assemblies
|
45
|
+
autoload :Mm9, "#{base}/mm9"
|
46
|
+
autoload :Mm10, "#{base}/mm10"
|
47
|
+
|
48
|
+
# rat genome assemblies
|
49
|
+
autoload :Rn5, "#{base}/rn5"
|
50
|
+
autoload :Rn4, "#{base}/rn4"
|
51
|
+
|
52
|
+
# guinea pig genome assemblies
|
53
|
+
autoload :CavPor3, "#{base}/cavpor3"
|
54
|
+
|
55
|
+
# rabbit genome assemblies
|
56
|
+
autoload :OryCun2, "#{base}/orycun2"
|
57
|
+
|
58
|
+
# cat genome assemblies
|
59
|
+
autoload :FelCat4, "#{base}/felcat4"
|
60
|
+
|
61
|
+
# panda genome assemblies
|
62
|
+
autoload :AilMel1, "#{base}/ailmel1"
|
63
|
+
|
64
|
+
# dog genome assemblies
|
65
|
+
autoload :CanFam2, "#{base}/canfam2"
|
66
|
+
|
67
|
+
# horse genome assemblies
|
68
|
+
autoload :EquCab2, "#{base}/equcab2"
|
69
|
+
|
70
|
+
# pig genome assemblies
|
71
|
+
autoload :SusScr2, "#{base}/susscr2"
|
72
|
+
|
73
|
+
# sheep genome assemblies
|
74
|
+
autoload :OviAri1, "#{base}/oviari1"
|
75
|
+
|
76
|
+
# cow genome assemblies
|
77
|
+
autoload :BosTau4, "#{base}/bostau4"
|
78
|
+
|
79
|
+
# elephant genome assemblies
|
80
|
+
autoload :LoxAfr3, "#{base}/loxafr3"
|
81
|
+
|
82
|
+
# opossum genome assemblies
|
83
|
+
autoload :MonDom5, "#{base}/mondom5"
|
84
|
+
|
85
|
+
# platypus genome assemblies
|
86
|
+
autoload :OrnAna1, "#{base}/ornana1"
|
87
|
+
|
88
|
+
# vertebrates #################################
|
89
|
+
# chiken genome assemblies
|
90
|
+
autoload :GalGal4, "#{base}/galgal4"
|
91
|
+
|
92
|
+
# zebra finch genome assemblies
|
93
|
+
autoload :TaeGut1, "#{base}/taegut1"
|
94
|
+
|
95
|
+
# lizard genome assemblies
|
96
|
+
autoload :AnoCar2, "#{base}/anocar2"
|
97
|
+
|
98
|
+
# X. tropicalis genome assemblies
|
99
|
+
autoload :XenTro2, "#{base}/xentro2"
|
100
|
+
|
101
|
+
# zebrafish genome assemblies
|
102
|
+
autoload :DanRer7, "#{base}/danrer7"
|
103
|
+
|
104
|
+
# tetraodon genome assemblies
|
105
|
+
autoload :TetNig2, "#{base}/tetnig2"
|
106
|
+
|
107
|
+
# fugu genome assemblies
|
108
|
+
autoload :Fr2, "#{base}/fr2"
|
109
|
+
|
110
|
+
# stickleback genome assemblies
|
111
|
+
autoload :GasAcu1, "#{base}/gasacu1"
|
112
|
+
|
113
|
+
# medaka genome assemblies
|
114
|
+
autoload :OryLat2, "#{base}/orylat2"
|
115
|
+
|
116
|
+
# lamprey genome assemblies
|
117
|
+
autoload :PetMar1, "#{base}/petmar1"
|
118
|
+
|
119
|
+
# deuterostomes ###############################
|
120
|
+
# lancelet genome assemblies
|
121
|
+
autoload :BraFlo1, "#{base}/braflo1.rb"
|
122
|
+
|
123
|
+
# sea squirt genome assemblies
|
124
|
+
autoload :Ci2, "#{base}/ci2"
|
125
|
+
|
126
|
+
# sea urchin genome assemblies
|
127
|
+
autoload :StrPur2, "#{base}/strpur2"
|
128
|
+
|
129
|
+
# insects ####################################
|
130
|
+
# D. melanogaster genome assemblies
|
131
|
+
autoload :Dm3, "#{base}/dm3"
|
132
|
+
|
133
|
+
# D. silulans genome assemblies
|
134
|
+
autoload :DroSim1, "#{base}/drosim1"
|
135
|
+
|
136
|
+
# D. sechellia genome assemblies
|
137
|
+
autoload :DroSec1, "#{base}/drosec1"
|
138
|
+
|
139
|
+
# D. yakuba genome assemblies
|
140
|
+
autoload :DroYak2, "#{base}/droyak2"
|
141
|
+
|
142
|
+
# D. erecta genome assemblies
|
143
|
+
autoload :DroEre1, "#{base}/droere1"
|
144
|
+
|
145
|
+
# D. ananassae genome assemblies
|
146
|
+
autoload :DroAna2, "#{base}/droana2"
|
147
|
+
|
148
|
+
# D. pseudoobscura genome assemblies
|
149
|
+
autoload :Dp3, "#{base}/dp3"
|
150
|
+
|
151
|
+
# D. persimilis genome assemblies
|
152
|
+
autoload :DroPer1, "#{base}/droper1"
|
153
|
+
|
154
|
+
# D. virilis genome assemblies
|
155
|
+
autoload :DroVir2, "#{base}/drovir2"
|
156
|
+
|
157
|
+
# D. mojavensis genome assemblies
|
158
|
+
autoload :DroMoj2, "#{base}/dromoj2"
|
159
|
+
|
160
|
+
# D. grimshawi genome assemblies
|
161
|
+
autoload :DroGri1, "#{base}/drogri1"
|
162
|
+
|
163
|
+
# Anopheles mosquito genome assemblies
|
164
|
+
autoload :AnoGam1, "#{base}/anogam1"
|
165
|
+
|
166
|
+
# honey bee genome assemblies
|
167
|
+
autoload :ApiMel2, "#{base}/apimel2"
|
168
|
+
|
169
|
+
# nematodes ###################################
|
170
|
+
# C. elegans genome assemblies
|
171
|
+
autoload :Ce6, "#{base}/ce6"
|
172
|
+
|
173
|
+
# C. brenneri genome assemblies
|
174
|
+
autoload :CaePb2, "#{base}/caepb2"
|
175
|
+
|
176
|
+
# C. briggsae genome assemblies
|
177
|
+
autoload :Cb3, "#{base}/cb3"
|
178
|
+
|
179
|
+
# C. remanei genome assemblies
|
180
|
+
autoload :CaeRem3, "#{base}/caerem3"
|
181
|
+
|
182
|
+
# C. japonica genome assemblies
|
183
|
+
autoload :CaeJap1, "#{base}/caejap1"
|
184
|
+
|
185
|
+
# P. pacificus genome assemblies
|
186
|
+
autoload :PriPac1, "#{base}/pripac1"
|
187
|
+
|
188
|
+
# others #####################################
|
189
|
+
# sea hare genome assemblies
|
190
|
+
autoload :AplCal1, "#{base}/aplcal1"
|
191
|
+
|
192
|
+
# yeast genome assemblies
|
193
|
+
autoload :SacCer2, "#{base}/saccer2"
|
194
|
+
|
195
|
+
# genome assembly independent dababases #######
|
196
|
+
autoload :UniProt, "#{base}/uniprot"
|
197
|
+
autoload :Proteome, "#{base}/proteome"
|
198
|
+
autoload :Go, "#{base}/go"
|
199
|
+
autoload :HgFixed, "#{base}/hgfixed"
|
200
|
+
autoload :VisiGene, "#{base}/visigene"
|
201
|
+
|
202
|
+
# utility classes #############################
|
203
|
+
# accessing genome sequences in the 2bit format
|
204
|
+
# after version 0.4.0, UCSC::Reference is moved to UCSC::File::Twobit
|
205
|
+
autoload :File, "#{base}/file"
|
206
|
+
autoload :Reference, "#{base}/reference"
|
207
|
+
autoload :Schema, "#{base}/schema"
|
208
|
+
end
|
209
|
+
end
|
data/lib/bio-ucsc.rb
CHANGED
@@ -7,17 +7,21 @@
|
|
7
7
|
base = "#{::File.dirname(__FILE__)}/bio-ucsc"
|
8
8
|
|
9
9
|
require 'rubygems'
|
10
|
-
gem 'activerecord', "
|
10
|
+
gem 'activerecord', "~> 4.0.2"
|
11
11
|
require "active_record"
|
12
12
|
gem "safe_attributes"
|
13
13
|
require "safe_attributes"
|
14
|
+
gem "activerecord-deprecated_finders"
|
15
|
+
require "active_record/deprecated_finders"
|
16
|
+
#ActiveSupport::Deprecation.silenced = true
|
17
|
+
|
14
18
|
require "#{base}/ucsc_bin"
|
15
19
|
require "#{base}/genomic-interval-bin"
|
16
20
|
require "#{base}/gi.rb"
|
17
21
|
|
18
22
|
module Bio
|
19
23
|
module Ucsc
|
20
|
-
VERSION = "0.
|
24
|
+
VERSION = "0.6.0"
|
21
25
|
base = "#{::File.dirname(__FILE__)}/bio-ucsc"
|
22
26
|
|
23
27
|
# mammmals #####################################
|
data/samples/bed2refseq.rb
CHANGED
@@ -1,11 +1,10 @@
|
|
1
|
-
#!/usr/
|
1
|
+
#!/usr/bin/env ruby
|
2
2
|
|
3
3
|
#require 'bio-ucsc'
|
4
4
|
require '../lib/bio-ucsc'
|
5
5
|
|
6
|
-
|
7
|
-
|
8
|
-
Ucsc::Hg19::DBConnection.connect
|
6
|
+
DB = Bio::Ucsc::Hg19
|
7
|
+
DB.connect
|
9
8
|
|
10
9
|
genes = Array.new
|
11
10
|
ARGF.each_line do |row|
|
@@ -16,7 +15,7 @@ ARGF.each_line do |row|
|
|
16
15
|
Integer(chr_start),
|
17
16
|
Integer(chr_end),)
|
18
17
|
|
19
|
-
results =
|
18
|
+
results = DB.with_interval(gi).select(:name2).find(:all)
|
20
19
|
genes.concat(results.map{|e|e.name2})
|
21
20
|
end
|
22
21
|
|
@@ -16,12 +16,11 @@ HG19_2BIT_FILE = "hg19.2bit"
|
|
16
16
|
require File.dirname(__FILE__) + '/../lib/bio-ucsc'
|
17
17
|
|
18
18
|
class Hg19Ref
|
19
|
-
|
19
|
+
DB = Bio::Ucsc::Hg19
|
20
20
|
|
21
21
|
def run(interval)
|
22
|
-
|
22
|
+
DB.connect
|
23
23
|
ref = Bio::Ucsc::Reference.load(HG19_2BIT_FILE) # v0.2 and later
|
24
|
-
|
25
24
|
itv = Bio::GenomicInterval.parse(interval)
|
26
25
|
|
27
26
|
puts itv.to_s
|
data/samples/num-gene-exon.rb
CHANGED
@@ -13,9 +13,10 @@ require 'bio-ucsc'
|
|
13
13
|
|
14
14
|
interval = Bio::GenomicInterval.parse(ARGV[0])
|
15
15
|
|
16
|
-
Bio::Ucsc::Hg18
|
16
|
+
DB = Bio::Ucsc::Hg18
|
17
|
+
DB.connect
|
17
18
|
|
18
|
-
genes =
|
19
|
+
genes = DB::RefGene.with_interval(interval).find(:all).map{|e|e.name2}.uniq
|
19
20
|
puts "Included genes:"
|
20
21
|
puts genes
|
21
22
|
puts "Number of genes:"
|
@@ -24,7 +25,7 @@ puts genes.size
|
|
24
25
|
total_exons = 0
|
25
26
|
genes.each do |gene|
|
26
27
|
total_exons +=
|
27
|
-
|
28
|
+
DB::RefGene.
|
28
29
|
with_interval(interval).
|
29
30
|
find_all_by_name2(gene).
|
30
31
|
map{|e|e.exonCount}.
|
metadata
CHANGED
@@ -1,7 +1,7 @@
|
|
1
1
|
--- !ruby/object:Gem::Specification
|
2
2
|
name: bio-ucsc-api
|
3
3
|
version: !ruby/object:Gem::Version
|
4
|
-
version: 0.
|
4
|
+
version: 0.6.0
|
5
5
|
prerelease:
|
6
6
|
platform: ruby
|
7
7
|
authors:
|
@@ -10,66 +10,77 @@ authors:
|
|
10
10
|
autorequire:
|
11
11
|
bindir: bin
|
12
12
|
cert_chain: []
|
13
|
-
date: 2013-
|
13
|
+
date: 2013-12-11 00:00:00.000000000 Z
|
14
14
|
dependencies:
|
15
15
|
- !ruby/object:Gem::Dependency
|
16
16
|
name: activerecord
|
17
|
-
requirement: &
|
17
|
+
requirement: &160170600 !ruby/object:Gem::Requirement
|
18
18
|
none: false
|
19
19
|
requirements:
|
20
20
|
- - ~>
|
21
21
|
- !ruby/object:Gem::Version
|
22
|
-
version:
|
22
|
+
version: 4.0.2
|
23
23
|
type: :runtime
|
24
24
|
prerelease: false
|
25
|
-
version_requirements: *
|
25
|
+
version_requirements: *160170600
|
26
26
|
- !ruby/object:Gem::Dependency
|
27
27
|
name: activesupport
|
28
|
-
requirement: &
|
28
|
+
requirement: &160169480 !ruby/object:Gem::Requirement
|
29
29
|
none: false
|
30
30
|
requirements:
|
31
31
|
- - ~>
|
32
32
|
- !ruby/object:Gem::Version
|
33
|
-
version:
|
33
|
+
version: 4.0.2
|
34
34
|
type: :runtime
|
35
35
|
prerelease: false
|
36
|
-
version_requirements: *
|
36
|
+
version_requirements: *160169480
|
37
37
|
- !ruby/object:Gem::Dependency
|
38
38
|
name: mysql
|
39
|
-
requirement: &
|
39
|
+
requirement: &160168120 !ruby/object:Gem::Requirement
|
40
40
|
none: false
|
41
41
|
requirements:
|
42
42
|
- - ~>
|
43
43
|
- !ruby/object:Gem::Version
|
44
|
-
version: 2.
|
44
|
+
version: 2.9.1
|
45
45
|
type: :runtime
|
46
46
|
prerelease: false
|
47
|
-
version_requirements: *
|
47
|
+
version_requirements: *160168120
|
48
48
|
- !ruby/object:Gem::Dependency
|
49
|
-
name:
|
50
|
-
requirement: &
|
49
|
+
name: safe_attributes
|
50
|
+
requirement: &160166800 !ruby/object:Gem::Requirement
|
51
51
|
none: false
|
52
52
|
requirements:
|
53
|
-
- -
|
53
|
+
- - ~>
|
54
54
|
- !ruby/object:Gem::Version
|
55
|
-
version: 0.
|
55
|
+
version: 1.0.10
|
56
56
|
type: :runtime
|
57
57
|
prerelease: false
|
58
|
-
version_requirements: *
|
58
|
+
version_requirements: *160166800
|
59
59
|
- !ruby/object:Gem::Dependency
|
60
|
-
name:
|
61
|
-
requirement: &
|
60
|
+
name: activerecord-deprecated_finders
|
61
|
+
requirement: &160165900 !ruby/object:Gem::Requirement
|
62
|
+
none: false
|
63
|
+
requirements:
|
64
|
+
- - ~>
|
65
|
+
- !ruby/object:Gem::Version
|
66
|
+
version: 1.0.3
|
67
|
+
type: :runtime
|
68
|
+
prerelease: false
|
69
|
+
version_requirements: *160165900
|
70
|
+
- !ruby/object:Gem::Dependency
|
71
|
+
name: bio-genomic-interval
|
72
|
+
requirement: &160181120 !ruby/object:Gem::Requirement
|
62
73
|
none: false
|
63
74
|
requirements:
|
64
75
|
- - ! '>='
|
65
76
|
- !ruby/object:Gem::Version
|
66
|
-
version: 1.
|
77
|
+
version: 0.1.2
|
67
78
|
type: :runtime
|
68
79
|
prerelease: false
|
69
|
-
version_requirements: *
|
80
|
+
version_requirements: *160181120
|
70
81
|
- !ruby/object:Gem::Dependency
|
71
82
|
name: rake
|
72
|
-
requirement: &
|
83
|
+
requirement: &160180180 !ruby/object:Gem::Requirement
|
73
84
|
none: false
|
74
85
|
requirements:
|
75
86
|
- - ~>
|
@@ -77,21 +88,21 @@ dependencies:
|
|
77
88
|
version: 10.1.0
|
78
89
|
type: :development
|
79
90
|
prerelease: false
|
80
|
-
version_requirements: *
|
91
|
+
version_requirements: *160180180
|
81
92
|
- !ruby/object:Gem::Dependency
|
82
93
|
name: rspec
|
83
|
-
requirement: &
|
94
|
+
requirement: &160179160 !ruby/object:Gem::Requirement
|
84
95
|
none: false
|
85
96
|
requirements:
|
86
97
|
- - ~>
|
87
98
|
- !ruby/object:Gem::Version
|
88
|
-
version: 2.
|
99
|
+
version: 2.14.1
|
89
100
|
type: :development
|
90
101
|
prerelease: false
|
91
|
-
version_requirements: *
|
102
|
+
version_requirements: *160179160
|
92
103
|
- !ruby/object:Gem::Dependency
|
93
104
|
name: bundler
|
94
|
-
requirement: &
|
105
|
+
requirement: &160178180 !ruby/object:Gem::Requirement
|
95
106
|
none: false
|
96
107
|
requirements:
|
97
108
|
- - ~>
|
@@ -99,57 +110,58 @@ dependencies:
|
|
99
110
|
version: 1.3.5
|
100
111
|
type: :development
|
101
112
|
prerelease: false
|
102
|
-
version_requirements: *
|
113
|
+
version_requirements: *160178180
|
103
114
|
- !ruby/object:Gem::Dependency
|
104
115
|
name: jeweler
|
105
|
-
requirement: &
|
116
|
+
requirement: &160177160 !ruby/object:Gem::Requirement
|
106
117
|
none: false
|
107
118
|
requirements:
|
108
119
|
- - ~>
|
109
120
|
- !ruby/object:Gem::Version
|
110
|
-
version: 1.8.
|
121
|
+
version: 1.8.8
|
111
122
|
type: :development
|
112
123
|
prerelease: false
|
113
|
-
version_requirements: *
|
124
|
+
version_requirements: *160177160
|
114
125
|
- !ruby/object:Gem::Dependency
|
115
126
|
name: bio
|
116
|
-
requirement: &
|
127
|
+
requirement: &160176000 !ruby/object:Gem::Requirement
|
117
128
|
none: false
|
118
129
|
requirements:
|
119
130
|
- - ! '>='
|
120
131
|
- !ruby/object:Gem::Version
|
121
|
-
version: 1.4.
|
132
|
+
version: 1.4.3
|
122
133
|
type: :development
|
123
134
|
prerelease: false
|
124
|
-
version_requirements: *
|
135
|
+
version_requirements: *160176000
|
125
136
|
- !ruby/object:Gem::Dependency
|
126
137
|
name: rdoc
|
127
|
-
requirement: &
|
138
|
+
requirement: &160175260 !ruby/object:Gem::Requirement
|
128
139
|
none: false
|
129
140
|
requirements:
|
130
141
|
- - ! '>='
|
131
142
|
- !ruby/object:Gem::Version
|
132
|
-
version:
|
143
|
+
version: 4.0.1
|
133
144
|
type: :development
|
134
145
|
prerelease: false
|
135
|
-
version_requirements: *
|
146
|
+
version_requirements: *160175260
|
136
147
|
description: ! 'Ruby UCSC API: accessing the UCSC Genome Database using Ruby'
|
137
148
|
email: missy@be.to
|
138
149
|
executables: []
|
139
150
|
extensions: []
|
140
151
|
extra_rdoc_files:
|
141
152
|
- ChangeLog.md
|
153
|
+
- LICENSE.txt
|
142
154
|
- README.md
|
143
155
|
files:
|
144
|
-
- COPYING
|
145
|
-
- COPYING.ja
|
146
156
|
- ChangeLog.md
|
147
157
|
- Gemfile
|
148
158
|
- Gemfile.lock
|
159
|
+
- LICENSE.txt
|
149
160
|
- README.md
|
150
161
|
- Rakefile
|
151
162
|
- VERSION
|
152
163
|
- bio-ucsc-api.gemspec
|
164
|
+
- lib/#bio-ucsc.rb#
|
153
165
|
- lib/bio-ucsc-api.rb
|
154
166
|
- lib/bio-ucsc.rb
|
155
167
|
- lib/bio-ucsc/ailmel1.rb
|
@@ -557,7 +569,7 @@ files:
|
|
557
569
|
- samples/symbol2summary.rb
|
558
570
|
homepage: http://github.com/misshie/bioruby-ucsc-api
|
559
571
|
licenses:
|
560
|
-
-
|
572
|
+
- The MIT License
|
561
573
|
post_install_message:
|
562
574
|
rdoc_options: []
|
563
575
|
require_paths:
|
@@ -570,7 +582,7 @@ required_ruby_version: !ruby/object:Gem::Requirement
|
|
570
582
|
version: '0'
|
571
583
|
segments:
|
572
584
|
- 0
|
573
|
-
hash:
|
585
|
+
hash: -3654614311172701481
|
574
586
|
required_rubygems_version: !ruby/object:Gem::Requirement
|
575
587
|
none: false
|
576
588
|
requirements:
|
data/COPYING
DELETED
@@ -1,58 +0,0 @@
|
|
1
|
-
BioRuby is copyrighted free software by
|
2
|
-
Hiroyuki Mishima <missy at be.to> / <hmishima at nagasaki-u.ac.jp>, and
|
3
|
-
Jan Aerts <jan.aerts@gmail.com>.
|
4
|
-
You can redistribute it and/or modify it under either the terms of the GPL
|
5
|
-
version 2 (see the file GPL), or the conditions below:
|
6
|
-
|
7
|
-
1. You may make and give away verbatim copies of the source form of the
|
8
|
-
software without restriction, provided that you duplicate all of the
|
9
|
-
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software (possibly commercial). But some files in the distribution
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are not written by the author, so that they are not under these terms.
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