bio-samtools 0.4.1 → 0.4.2
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- data/VERSION +1 -1
 - data/bio-samtools.gemspec +60 -6
 - data/doc/Bio.html +171 -0
 - data/doc/Bio/DB.html +163 -0
 - data/doc/Bio/DB/Alignment.html +780 -0
 - data/doc/Bio/DB/SAM.html +1241 -0
 - data/doc/Bio/DB/SAM/Library.html +217 -0
 - data/doc/Bio/DB/SAM/Tools.html +331 -0
 - data/doc/Bio/DB/SAM/Tools/Bam1CoreT.html +159 -0
 - data/doc/Bio/DB/SAM/Tools/Bam1T.html +220 -0
 - data/doc/Bio/DB/SAM/Tools/BamHeaderT.html +249 -0
 - data/doc/Bio/DB/SAM/Tools/BamPileup1T.html +159 -0
 - data/doc/Bio/DB/SAM/Tools/SamfileT.html +171 -0
 - data/doc/Bio/DB/SAM/Tools/SamfileTX.html +159 -0
 - data/doc/Bio/DB/SAMException.html +205 -0
 - data/doc/Bio/DB/Tag.html +254 -0
 - data/doc/LICENSE_txt.html +881 -0
 - data/doc/LibC.html +153 -0
 - data/doc/Pileup.html +571 -0
 - data/doc/Vcf.html +473 -0
 - data/doc/created.rid +10 -1
 - data/doc/images/add.png +0 -0
 - data/doc/images/brick.png +0 -0
 - data/doc/images/brick_link.png +0 -0
 - data/doc/images/bug.png +0 -0
 - data/doc/images/bullet_black.png +0 -0
 - data/doc/images/bullet_toggle_minus.png +0 -0
 - data/doc/images/bullet_toggle_plus.png +0 -0
 - data/doc/images/date.png +0 -0
 - data/doc/images/delete.png +0 -0
 - data/doc/images/find.png +0 -0
 - data/doc/images/loadingAnimation.gif +0 -0
 - data/doc/images/macFFBgHack.png +0 -0
 - data/doc/images/package.png +0 -0
 - data/doc/images/page_green.png +0 -0
 - data/doc/images/page_white_text.png +0 -0
 - data/doc/images/page_white_width.png +0 -0
 - data/doc/images/plugin.png +0 -0
 - data/doc/images/ruby.png +0 -0
 - data/doc/images/tag_blue.png +0 -0
 - data/doc/images/tag_green.png +0 -0
 - data/doc/images/transparent.png +0 -0
 - data/doc/images/wrench.png +0 -0
 - data/doc/images/wrench_orange.png +0 -0
 - data/doc/images/zoom.png +0 -0
 - data/doc/index.html +109 -21
 - data/doc/js/darkfish.js +153 -0
 - data/doc/js/jquery.js +18 -0
 - data/doc/js/navigation.js +142 -0
 - data/doc/js/search.js +94 -0
 - data/doc/js/search_index.js +1 -0
 - data/doc/js/searcher.js +228 -0
 - data/doc/lib/bio-samtools_rb.html +115 -0
 - data/doc/lib/bio/db/sam/bam_rb.html +121 -0
 - data/doc/lib/bio/db/sam/faidx_rb.html +117 -0
 - data/doc/lib/bio/db/sam/library_rb.html +115 -0
 - data/doc/lib/bio/db/sam/pileup_rb.html +171 -0
 - data/doc/lib/bio/db/sam/sam_rb.html +121 -0
 - data/doc/lib/bio/db/sam/vcf_rb.html +124 -0
 - data/doc/lib/bio/db/sam_rb.html +115 -0
 - data/doc/rdoc.css +543 -0
 - data/doc/table_of_contents.html +186 -0
 - data/ext/Makefile-bioruby.patch +1 -1
 - data/ext/Rakefile +8 -1
 - data/ext/mkrf_conf.rb +8 -1
 - data/lib/bio/db/sam.rb +140 -27
 - data/lib/bio/db/sam/vcf.rb +0 -1
 - data/test/basictest.rb +4 -4
 - data/test/test_basic.rb +50 -34
 - metadata +79 -25
 - data/test/coverage.rb +0 -26
 - data/test/coverage_plot.rb +0 -28
 - data/test/feature.rb +0 -0
 - data/test/test_pileup.rb +0 -68
 
    
        data/test/coverage.rb
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            $: << File.expand_path(File.dirname(__FILE__) + '/../lib')
         
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            $: << File.expand_path('.')
         
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            require "bio/db/sam"
         
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            require "bio/db/sam/sam"
         
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            bam_file = ARGV[0]
         
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            fasta_file = ARGV[1]
         
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            chromosmes = ARGV[2]
         
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            i = 0
         
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            sam       = Bio::DB::Sam.new({:bam=>bam_file, :fasta=>fasta_file})
         
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            sam.open
         
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            File.open(chromosmes, "r") do |file|
         
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              file.each_line{|line|
         
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                $stderr.puts i.to_s + " done" if i%10 == 0
         
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                ObjectSpace.each_object(Bio::DB::Sam) {|x|  $stderr.puts x } if i%10 == 0
         
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                count =  ObjectSpace.each_object() {|x|  } if i%10 == 0
         
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                $stderr.puts count if i%10 == 0
         
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                i = i + 1
         
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                fetching = line.split(' ')[0]
         
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            	  cov = sam.average_coverage(fetching, 0, 16000)
         
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            	  puts fetching + " " + cov.to_s
         
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              }
         
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            end
         
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            sam.close
         
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        data/test/coverage_plot.rb
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            $: << File.expand_path(File.dirname(__FILE__) + '/../lib')
         
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            $: << File.expand_path('.')
         
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            require "bio/db/sam"
         
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            require "bio/db/sam/sam"
         
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            bam_file = ARGV[0]
         
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            fasta_file = ARGV[1]
         
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            chromosmes = ARGV[2]
         
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            sam       = Bio::DB::Sam.new({:bam=>bam_file, :fasta=>fasta_file})
         
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            sam.open
         
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            File.open(chromosmes, "r") do |file|
         
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                file.each_line{|line|
         
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                  fetching = line.split()[0]
         
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            	    last = line.split()[1].to_i
         
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            	    covs = sam.chromosome_coverage(fetching, 0, last)
         
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            	    puts "POS\tCOV"
         
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            	   # puts "#{fetching}\t#{last}"
         
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            	    covs.each_with_index{ |cov, i| puts "#{i}\t#{cov}" }
         
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                }
         
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            end
         
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            sam.close
         
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        data/test/feature.rb
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        data/test/test_pileup.rb
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            $: << File.expand_path(File.dirname(__FILE__) + '/../lib')
         
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            $: << File.expand_path('.')
         
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            require "bio/db/sam/pileup"
         
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            require "test/unit"
         
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            class TestPileup < Test::Unit::TestCase
         
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              def setup
         
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                @six_col = Pileup.new("seq1\t272\tT\t24\t,.$.....,,.,.,...,,,.,..^+.\t<<<+;<<<<<<<<<<<=<;<;7<&")
         
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                @ten_col = Pileup.new("seq2\t151\tG\tG\t36\t0\t99\t12\t...........A\t:9<;;7=<<<<<")
         
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                @snp = Pileup.new("seq1\t272\tT\t24\t,.$.....,,.gGgGgGgGgGgGg^+.\t<<<+;<<<<<<<<<<<=<;<;7<&") 
         
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                @snp_2 = Pileup.new("seq1\t272\tT\t24\t......aaaaaaggggggcccccc$^+\t<<<+;<<<<<<<<<<<=<;<;7<&")
         
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                @snp_3 = Pileup.new("seq1\t272\tT\t24\t......aaaaaaaagggggccccc$^+\t<<<+;<<<<<<<<<<<=<;<;7<&")
         
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              end
         
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              def test_new_from_6_column
         
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                assert_equal("seq1", @six_col.ref_name)
         
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                assert_equal(272, @six_col.pos)
         
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                assert_equal("T", @six_col.ref_base)
         
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                assert_equal(24, @six_col.coverage)
         
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                assert_equal(",.$.....,,.,.,...,,,.,..^+.", @six_col.read_bases)
         
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                assert_equal("<<<+;<<<<<<<<<<<=<;<;7<&", @six_col.read_quals)
         
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              end
         
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              def test_new_from_10_column
         
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                assert_equal("seq2", @ten_col.ref_name)
         
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                assert_equal(151, @ten_col.pos)
         
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                assert_equal("G", @ten_col.ref_base)
         
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                assert_equal("G", @ten_col.consensus)
         
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                assert_equal(36, @ten_col.consensus_quality)
         
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                assert_equal(0, @ten_col.snp_quality)
         
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                assert_equal(99, @ten_col.rms_mapq)
         
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                assert_equal(12, @ten_col.coverage)
         
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                assert_equal("...........A", @ten_col.read_bases)
         
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                assert_equal(":9<;;7=<<<<<", @ten_col.read_quals)
         
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              end
         
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              def test_non_refs
         
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                assert_equal({:A => 1, :C => 0, :T => 0, :G => 0}, @ten_col.non_refs)
         
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                assert_equal({:A => 0, :C => 0, :T => 0, :G => 0}, @six_col.non_refs)
         
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              end
         
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              def test_consensus
         
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                assert_equal("G", @snp.consensus)
         
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                assert_equal("ACGT", @snp_2.consensus)
         
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                assert_equal("A", @snp_3.consensus)
         
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              end
         
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              def test_non_ref_count
         
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                assert_equal(13,@snp.non_ref_count)
         
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                assert_equal(18,@snp_2.non_ref_count)
         
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                assert_equal(18,@snp_3.non_ref_count)
         
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              end
         
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              def test_ref_count
         
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                assert_equal(11,@snp.ref_count)
         
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                assert_equal(6,@snp_2.ref_count)
         
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                assert_equal(6,@snp_3.ref_count)
         
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              end
         
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              def test_ref_plus_non_ref_equal_to_coverage
         
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                assert_equal(@snp.coverage,@snp.ref_count + @snp.non_ref_count)
         
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               assert_equal(@snp_2.coverage,@snp_2.ref_count + @snp_2.non_ref_count)
         
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               assert_equal(@snp_3.coverage,@snp_3.ref_count + @snp_3.non_ref_count)
         
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              end
         
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            end
         
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