scanitd 0.9.0__tar.gz
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- scanitd-0.9.0/LICENSE +21 -0
- scanitd-0.9.0/PKG-INFO +103 -0
- scanitd-0.9.0/README.md +72 -0
- scanitd-0.9.0/pyproject.toml +209 -0
- scanitd-0.9.0/setup.py +49 -0
- scanitd-0.9.0/src/scanitd/__init__.py +5 -0
- scanitd-0.9.0/src/scanitd/base/__init__.py +27 -0
- scanitd-0.9.0/src/scanitd/base/basic.py +505 -0
- scanitd-0.9.0/src/scanitd/base/basic_read.py +184 -0
- scanitd-0.9.0/src/scanitd/base/cigar.py +52 -0
- scanitd-0.9.0/src/scanitd/cli/__init__.py +3 -0
- scanitd-0.9.0/src/scanitd/cli/cli.py +152 -0
- scanitd-0.9.0/src/scanitd/inference/__init__.py +6 -0
- scanitd-0.9.0/src/scanitd/inference/helper.py +494 -0
- scanitd-0.9.0/src/scanitd/inference/main.py +399 -0
- scanitd-0.9.0/src/scanitd/inference/sr_resuer.py +184 -0
- scanitd-0.9.0/src/scanitd/mtype.py +32 -0
- scanitd-0.9.0/src/scanitd/py.typed +0 -0
- scanitd-0.9.0/src/scanitd/writer/__init__.py +6 -0
- scanitd-0.9.0/src/scanitd/writer/vcf_writer.py +287 -0
- scanitd-0.9.0/src/scanitd/writer/writer.py +45 -0
scanitd-0.9.0/LICENSE
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MIT License
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Copyright (c) 2024, Ting-You Wang
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Permission is hereby granted, free of charge, to any person obtaining a copy
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of this software and associated documentation files (the "Software"), to deal
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in the Software without restriction, including without limitation the rights
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to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
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copies of the Software, and to permit persons to whom the Software is
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furnished to do so, subject to the following conditions:
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The above copyright notice and this permission notice shall be included in all
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copies or substantial portions of the Software.
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THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
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IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
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FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
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AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
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LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
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OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
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SOFTWARE.
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scanitd-0.9.0/PKG-INFO
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Metadata-Version: 2.1
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Name: scanitd
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Version: 0.9.0
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Summary: ScanITD
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Home-page: https://github.com/ylab-hi/ScanITD
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License: MIT
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Author: Ting-You Wang
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Author-email: tywang@northwestern.edu
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Requires-Python: >=3.9,<4.0
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Classifier: Development Status :: 4 - Beta
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Classifier: License :: OSI Approved :: MIT License
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Classifier: Programming Language :: Python :: 3
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Classifier: Programming Language :: Python :: 3.9
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Classifier: Programming Language :: Python :: 3.10
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Classifier: Programming Language :: Python :: 3.11
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Classifier: Programming Language :: Python :: 3.12
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Classifier: Programming Language :: Python :: 3.13
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Requires-Dist: loguru (>=0.7.0,<0.8.0)
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Requires-Dist: numpy (>=2.0.0,<3.0.0)
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Requires-Dist: psutil (>=5.9.5,<6.0.0)
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Requires-Dist: pyfaidx (>=0.7.2,<0.8.0)
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Requires-Dist: pysam (>=0.22.0,<0.23.0)
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Requires-Dist: rich (>=13.7.0,<14.0.0)
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Requires-Dist: ssw-py (>=1.0.1,<2.0.0)
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Requires-Dist: typer (>=0.12.5,<0.13.0)
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Project-URL: Changelog, https://github.com/ylab-hi/ScanITD/releases
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Project-URL: Documentation, https://scanitd.readthedocs.io
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Project-URL: Repository, https://github.com/ylab-hi/ScanITD
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Description-Content-Type: text/markdown
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# ScanITD
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[](https://pypi.python.org/pypi/scanitd)
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[](https://pypi.org/project/scanitd/#files)
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[](https://github.com/ylab-hi/ScanITD/blob/main/LICENSE)
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- **PyPI**
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```bash
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pip install scanitd
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```
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# Usage
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Usage: scanitd [OPTIONS]
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ScanITD: Detecting internal tandem duplication with robust variant allele frequency estimation
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## Required Arguments
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* `--input`, `-i` PATH
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- Aligned BAM file
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- Required
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* `--ref`, `-r` PATH
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- Reference genome in FASTA format (with fai index)
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- Required
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* `--output`, `-o` TEXT
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- Output VCF file
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- Required
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## Optional Arguments
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* `--mapq`, `-m` INTEGER
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- Minimum MAPQ in BAM for calling ITD
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- Default: 15
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* `--ao`, `-c` INTEGER
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- Minimum observation count for ITD
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- Default: 4
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* `--depth`, `-d` INTEGER
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- Minimum depth to call ITD
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- Default: 10
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* `--vaf`, `-f` FLOAT
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- Minimum variant allele frequency
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- Default: 0.1
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* `--length` INTEGER
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- Minimum ITD length to report
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- Default: 10
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* `--aln-mismatches`, `-n` INTEGER
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- Maximum allowed mismatches for pairwise local alignment
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- Default: 1
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* `--ins-mismatches` INTEGER
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- Maximum allowed mismatches for insertion-inferred duplication
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- Default: 2
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* `--target`, `-t` TEXT
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- Limit analysis to targets listed in the BED-format file or a samtools region string
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* `--log-level`, `-l` [info|warning|error|debug|trace]
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- Set the logging level
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- Default: info
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* `--version`, `-v`
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- Show version and exit
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# Citation
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Wang TY. and Yang R. [ScanITD: Detecting internal tandem duplication with robust variant allele frequency estimation](https://doi.org/10.1093/gigascience/giaa089 "ScanITD: Detecting internal tandem duplication with robust variant allele frequency estimation").
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scanitd-0.9.0/README.md
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# ScanITD
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[](https://pypi.python.org/pypi/scanitd)
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[](https://pypi.org/project/scanitd/#files)
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[](https://github.com/ylab-hi/ScanITD/blob/main/LICENSE)
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- **PyPI**
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```bash
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pip install scanitd
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```
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# Usage
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Usage: scanitd [OPTIONS]
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ScanITD: Detecting internal tandem duplication with robust variant allele frequency estimation
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## Required Arguments
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* `--input`, `-i` PATH
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- Aligned BAM file
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- Required
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* `--ref`, `-r` PATH
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- Reference genome in FASTA format (with fai index)
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- Required
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* `--output`, `-o` TEXT
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- Output VCF file
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- Required
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## Optional Arguments
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* `--mapq`, `-m` INTEGER
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- Minimum MAPQ in BAM for calling ITD
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- Default: 15
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* `--ao`, `-c` INTEGER
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- Minimum observation count for ITD
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- Default: 4
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* `--depth`, `-d` INTEGER
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- Minimum depth to call ITD
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- Default: 10
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* `--vaf`, `-f` FLOAT
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- Minimum variant allele frequency
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- Default: 0.1
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* `--length` INTEGER
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- Minimum ITD length to report
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- Default: 10
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* `--aln-mismatches`, `-n` INTEGER
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- Maximum allowed mismatches for pairwise local alignment
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- Default: 1
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* `--ins-mismatches` INTEGER
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- Maximum allowed mismatches for insertion-inferred duplication
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- Default: 2
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* `--target`, `-t` TEXT
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- Limit analysis to targets listed in the BED-format file or a samtools region string
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* `--log-level`, `-l` [info|warning|error|debug|trace]
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- Set the logging level
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- Default: info
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* `--version`, `-v`
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- Show version and exit
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# Citation
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Wang TY. and Yang R. [ScanITD: Detecting internal tandem duplication with robust variant allele frequency estimation](https://doi.org/10.1093/gigascience/giaa089 "ScanITD: Detecting internal tandem duplication with robust variant allele frequency estimation").
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[tool.poetry]
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name = "scanitd"
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version = "0.9.0"
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description = "ScanITD"
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authors = [
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"Ting-You Wang <tywang@northwestern.edu>",
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]
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license = "MIT"
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readme = "README.md"
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homepage = "https://github.com/ylab-hi/ScanITD"
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repository = "https://github.com/ylab-hi/ScanITD"
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documentation = "https://scanitd.readthedocs.io"
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classifiers = [
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"Development Status :: 4 - Beta",
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"Programming Language :: Python :: 3.9",
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"Programming Language :: Python :: 3.10",
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"Programming Language :: Python :: 3.11",
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"Programming Language :: Python :: 3.12",
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]
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[tool.poetry.urls]
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Changelog = "https://github.com/ylab-hi/ScanITD/releases"
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[tool.poetry.dependencies]
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python = "^3.9"
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numpy = "^2.0.0"
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pysam = "^0.22.0"
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pyfaidx = "^0.7.2"
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psutil = "^5.9.5"
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ssw-py = "^1.0.1"
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loguru = "^0.7.0"
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typer = "^0.12.5"
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rich = "^13.7.0"
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[tool.poetry.extras]
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[tool.poetry.build]
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generate-setup-file = true
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[tool.poetry.scripts]
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scanitd = "scanitd.cli.cli:app"
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[tool.poetry.group.dev.dependencies]
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ruff = "^0.1.6"
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codespell = "^2.2.2"
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ipdb = "^0.13.11"
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isort = "^5.12.0"
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pytest = "^7.4.3"
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coverage = { extras = ["toml"], version = "^7.3" }
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safety = "^2.1.1"
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mypy = "^1.7"
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typeguard = "^4.1.5"
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xdoctest = { extras = ["colors"], version = "^1.1.2" }
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sphinx = "^7.2.6"
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myst-parser= "3.0.0"
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sphinx-autobuild = "^2021.3.14"
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pre-commit = "^3.3.3"
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flake8 = "^6.1.0"
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black = "^23.3"
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bandit = "^1.7.3"
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flake8-bandit = ">=4.1"
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flake8-bugbear = "^23.9.16"
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flake8-docstrings = "^1.6.0"
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flake8-rst-docstrings = "^0.3.0"
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pep8-naming = "^0.13.2"
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reorder-python-imports = "^3.8.2"
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pre-commit-hooks = "^4.3.0"
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sphinx-rtd-theme = "^1.3.0"
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sphinx-click = "^5.0.1"
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Pygments = "^2.17.1"
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pytest-mock = "^3.11.1"
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nox = "^2023.4.22"
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flake8-broken-line = "^1.0.0"
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flake8-comprehensions = "^3.13.0"
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flake8-eradicate = "^1.5.0"
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flake8-simplify = "^0.21.0"
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pynvim = "^0.4.3"
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flake8-2020 = "^1.8.1"
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flake8-alfred = "^1.1.1"
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cohesion = "^1.0.0"
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[tool.mypy]
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strict = false
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pretty = true
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show_column_numbers = true
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show_error_codes = true
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show_error_context = true
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exclude = "cppext"
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allow_untyped_calls = true
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ignore_missing_imports = true
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[tool.pytest.ini_options]
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norecursedirs = ["cppext"]
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[tool.bandit]
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exclude_dirs = ['tests']
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+
skips = ["B602", "B404", "B603"]
|
|
99
|
+
|
|
100
|
+
[tool.isort]
|
|
101
|
+
profile = "black"
|
|
102
|
+
|
|
103
|
+
[tool.ruff]
|
|
104
|
+
lint.select = [
|
|
105
|
+
# "ANN",
|
|
106
|
+
# "D",
|
|
107
|
+
"A",
|
|
108
|
+
"F",
|
|
109
|
+
"E",
|
|
110
|
+
"W",
|
|
111
|
+
"C90",
|
|
112
|
+
"I",
|
|
113
|
+
"UP",
|
|
114
|
+
"N",
|
|
115
|
+
"YTT",
|
|
116
|
+
"TID",
|
|
117
|
+
"S",
|
|
118
|
+
"BLE",
|
|
119
|
+
"FBT",
|
|
120
|
+
"PLR",
|
|
121
|
+
"B",
|
|
122
|
+
"B9",
|
|
123
|
+
"A",
|
|
124
|
+
"C4",
|
|
125
|
+
"T10",
|
|
126
|
+
"EM",
|
|
127
|
+
"ICN",
|
|
128
|
+
"T20",
|
|
129
|
+
"Q",
|
|
130
|
+
"RET",
|
|
131
|
+
"SIM",
|
|
132
|
+
"ARG",
|
|
133
|
+
"DTZ",
|
|
134
|
+
"ERA",
|
|
135
|
+
"PD",
|
|
136
|
+
"PGH",
|
|
137
|
+
"PLC",
|
|
138
|
+
"PLE",
|
|
139
|
+
"PLW",
|
|
140
|
+
"RUF",
|
|
141
|
+
"PL",
|
|
142
|
+
"TD",
|
|
143
|
+
"FIX",
|
|
144
|
+
"PTH",
|
|
145
|
+
"TCH",
|
|
146
|
+
"SLOT",
|
|
147
|
+
"PT",
|
|
148
|
+
"PYI",
|
|
149
|
+
"PIE",
|
|
150
|
+
"ISC",
|
|
151
|
+
"FA",
|
|
152
|
+
"EXE",
|
|
153
|
+
# "CPY",
|
|
154
|
+
"COM",
|
|
155
|
+
]
|
|
156
|
+
line-length = 200
|
|
157
|
+
exclude = ["tests"]
|
|
158
|
+
lint.ignore = [
|
|
159
|
+
"ANN001",
|
|
160
|
+
"D203",
|
|
161
|
+
"D213",
|
|
162
|
+
"PGH003",
|
|
163
|
+
"PLR0911", # Too many return statements
|
|
164
|
+
"PLR0913", # Too many arguments to function call
|
|
165
|
+
"PLR0912", # Too many branches
|
|
166
|
+
"PLR0915", # Too many statements
|
|
167
|
+
"DTZ005", # Without `tz` argument
|
|
168
|
+
"C901", # Too complex
|
|
169
|
+
'PLC0415',
|
|
170
|
+
'PLR1702',
|
|
171
|
+
'PLR0917',
|
|
172
|
+
# formater conflict
|
|
173
|
+
'COM812',
|
|
174
|
+
'COM819',
|
|
175
|
+
'D206',
|
|
176
|
+
'ISC001',
|
|
177
|
+
'Q000',
|
|
178
|
+
'Q001',
|
|
179
|
+
'Q002',
|
|
180
|
+
'Q003',
|
|
181
|
+
'W191',
|
|
182
|
+
"PLR0904",
|
|
183
|
+
]
|
|
184
|
+
target-version = "py310"
|
|
185
|
+
|
|
186
|
+
|
|
187
|
+
[tool.ruff.format]
|
|
188
|
+
# Like Black, use double quotes for strings.
|
|
189
|
+
quote-style = "double"
|
|
190
|
+
# Like Black, indent with spaces, rather than tabs.
|
|
191
|
+
indent-style = "space"
|
|
192
|
+
# Like Black, respect magic trailing commas.
|
|
193
|
+
# magic-trailing-comma = "respect"
|
|
194
|
+
# Like Black, automatically detect the appropriate line ending.
|
|
195
|
+
line-ending = "auto"
|
|
196
|
+
|
|
197
|
+
|
|
198
|
+
[tool.ruff.lint.per-file-ignores]
|
|
199
|
+
|
|
200
|
+
[tool.commitizen]
|
|
201
|
+
name = "cz_conventional_commits"
|
|
202
|
+
tag_format = "$version"
|
|
203
|
+
version_scheme = "pep440"
|
|
204
|
+
version_provider = "poetry"
|
|
205
|
+
major_version_zero = true
|
|
206
|
+
|
|
207
|
+
[build-system]
|
|
208
|
+
requires = ["poetry-core>=1.6.0", "setuptools>=68.0.0"]
|
|
209
|
+
build-backend = "poetry.core.masonry.api"
|
scanitd-0.9.0/setup.py
ADDED
|
@@ -0,0 +1,49 @@
|
|
|
1
|
+
# -*- coding: utf-8 -*-
|
|
2
|
+
from setuptools import setup
|
|
3
|
+
|
|
4
|
+
package_dir = \
|
|
5
|
+
{'': 'src'}
|
|
6
|
+
|
|
7
|
+
packages = \
|
|
8
|
+
['scanitd',
|
|
9
|
+
'scanitd.base',
|
|
10
|
+
'scanitd.cli',
|
|
11
|
+
'scanitd.inference',
|
|
12
|
+
'scanitd.writer']
|
|
13
|
+
|
|
14
|
+
package_data = \
|
|
15
|
+
{'': ['*']}
|
|
16
|
+
|
|
17
|
+
install_requires = \
|
|
18
|
+
['loguru>=0.7.0,<0.8.0',
|
|
19
|
+
'numpy>=2.0.0,<3.0.0',
|
|
20
|
+
'psutil>=5.9.5,<6.0.0',
|
|
21
|
+
'pyfaidx>=0.7.2,<0.8.0',
|
|
22
|
+
'pysam>=0.22.0,<0.23.0',
|
|
23
|
+
'rich>=13.7.0,<14.0.0',
|
|
24
|
+
'ssw-py>=1.0.1,<2.0.0',
|
|
25
|
+
'typer>=0.12.5,<0.13.0']
|
|
26
|
+
|
|
27
|
+
entry_points = \
|
|
28
|
+
{'console_scripts': ['scanitd = scanitd.cli.cli:app']}
|
|
29
|
+
|
|
30
|
+
setup_kwargs = {
|
|
31
|
+
'name': 'scanitd',
|
|
32
|
+
'version': '0.9.0',
|
|
33
|
+
'description': 'ScanITD',
|
|
34
|
+
'long_description': '# ScanITD\n\n[](https://pypi.python.org/pypi/scanitd)\n[](https://pypi.org/project/scanitd/#files)\n[](https://github.com/ylab-hi/ScanITD/blob/main/LICENSE)\n\n- **PyPI**\n\n```bash\npip install scanitd\n```\n\n# Usage\n\n Usage: scanitd [OPTIONS]\n\n ScanITD: Detecting internal tandem duplication with robust variant allele frequency estimation\n## Required Arguments\n* `--input`, `-i` PATH\n - Aligned BAM file\n - Required\n\n* `--ref`, `-r` PATH\n - Reference genome in FASTA format (with fai index)\n - Required\n\n* `--output`, `-o` TEXT\n - Output VCF file\n - Required\n\n## Optional Arguments\n* `--mapq`, `-m` INTEGER\n - Minimum MAPQ in BAM for calling ITD\n - Default: 15\n\n* `--ao`, `-c` INTEGER\n - Minimum observation count for ITD\n - Default: 4\n\n* `--depth`, `-d` INTEGER\n - Minimum depth to call ITD\n - Default: 10\n\n* `--vaf`, `-f` FLOAT\n - Minimum variant allele frequency\n - Default: 0.1\n\n* `--length` INTEGER\n - Minimum ITD length to report\n - Default: 10\n\n* `--aln-mismatches`, `-n` INTEGER\n - Maximum allowed mismatches for pairwise local alignment\n - Default: 1\n\n* `--ins-mismatches` INTEGER\n - Maximum allowed mismatches for insertion-inferred duplication\n - Default: 2\n\n* `--target`, `-t` TEXT\n - Limit analysis to targets listed in the BED-format file or a samtools region string\n\n* `--log-level`, `-l` [info|warning|error|debug|trace]\n - Set the logging level\n - Default: info\n\n* `--version`, `-v`\n - Show version and exit\n\n# Citation\n\nWang TY. and Yang R. [ScanITD: Detecting internal tandem duplication with robust variant allele frequency estimation](https://doi.org/10.1093/gigascience/giaa089 "ScanITD: Detecting internal tandem duplication with robust variant allele frequency estimation").\n',
|
|
35
|
+
'author': 'Ting-You Wang',
|
|
36
|
+
'author_email': 'tywang@northwestern.edu',
|
|
37
|
+
'maintainer': 'None',
|
|
38
|
+
'maintainer_email': 'None',
|
|
39
|
+
'url': 'https://github.com/ylab-hi/ScanITD',
|
|
40
|
+
'package_dir': package_dir,
|
|
41
|
+
'packages': packages,
|
|
42
|
+
'package_data': package_data,
|
|
43
|
+
'install_requires': install_requires,
|
|
44
|
+
'entry_points': entry_points,
|
|
45
|
+
'python_requires': '>=3.9,<4.0',
|
|
46
|
+
}
|
|
47
|
+
|
|
48
|
+
|
|
49
|
+
setup(**setup_kwargs)
|
|
@@ -0,0 +1,27 @@
|
|
|
1
|
+
"""Init file for scanitd2.
|
|
2
|
+
|
|
3
|
+
@Filename: __init__.py
|
|
4
|
+
"""
|
|
5
|
+
|
|
6
|
+
from .basic import (
|
|
7
|
+
CigarCode,
|
|
8
|
+
Event,
|
|
9
|
+
Interval,
|
|
10
|
+
Intervals,
|
|
11
|
+
MappingMode,
|
|
12
|
+
MicroRegion,
|
|
13
|
+
Strand,
|
|
14
|
+
)
|
|
15
|
+
from .basic_read import Read, reverse_complement
|
|
16
|
+
|
|
17
|
+
__all__ = [
|
|
18
|
+
"CigarCode",
|
|
19
|
+
"Event",
|
|
20
|
+
"Interval",
|
|
21
|
+
"Intervals",
|
|
22
|
+
"MappingMode",
|
|
23
|
+
"MicroRegion",
|
|
24
|
+
"Read",
|
|
25
|
+
"Strand",
|
|
26
|
+
"reverse_complement",
|
|
27
|
+
]
|