regrender 0.1.0__tar.gz

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Files changed (43) hide show
  1. regrender-0.1.0/.github/workflows/ci.yml +29 -0
  2. regrender-0.1.0/.github/workflows/publish.yml +27 -0
  3. regrender-0.1.0/.gitignore +195 -0
  4. regrender-0.1.0/.idea/.gitignore +8 -0
  5. regrender-0.1.0/.idea/2dccf.iml +18 -0
  6. regrender-0.1.0/.idea/deployment.xml +23 -0
  7. regrender-0.1.0/.idea/dictionaries/project.xml +7 -0
  8. regrender-0.1.0/.idea/inspectionProfiles/Project_Default.xml +8 -0
  9. regrender-0.1.0/.idea/inspectionProfiles/profiles_settings.xml +6 -0
  10. regrender-0.1.0/.idea/misc.xml +7 -0
  11. regrender-0.1.0/.idea/modules.xml +8 -0
  12. regrender-0.1.0/.idea/regrender.iml +7 -0
  13. regrender-0.1.0/.idea/vcs.xml +7 -0
  14. regrender-0.1.0/.python-version +1 -0
  15. regrender-0.1.0/.readthedocs.yaml +16 -0
  16. regrender-0.1.0/LICENSE +21 -0
  17. regrender-0.1.0/PKG-INFO +102 -0
  18. regrender-0.1.0/README.md +70 -0
  19. regrender-0.1.0/docs/Makefile +20 -0
  20. regrender-0.1.0/docs/make.bat +35 -0
  21. regrender-0.1.0/docs/source/_static/probe.png +0 -0
  22. regrender-0.1.0/docs/source/_static/register.png +0 -0
  23. regrender-0.1.0/docs/source/_static/roi.png +0 -0
  24. regrender-0.1.0/docs/source/conf.py +74 -0
  25. regrender-0.1.0/docs/source/get_start/data_structure.rst +87 -0
  26. regrender-0.1.0/docs/source/get_start/index.rst +27 -0
  27. regrender-0.1.0/docs/source/get_start/installation.rst +100 -0
  28. regrender-0.1.0/docs/source/get_start/probe.rst +72 -0
  29. regrender-0.1.0/docs/source/get_start/register.rst +69 -0
  30. regrender-0.1.0/docs/source/get_start/roi.rst +69 -0
  31. regrender-0.1.0/docs/source/index.rst +23 -0
  32. regrender-0.1.0/pyproject.toml +72 -0
  33. regrender-0.1.0/regrender/__init__.py +0 -0
  34. regrender-0.1.0/regrender/__main__.py +38 -0
  35. regrender-0.1.0/regrender/_app.py +326 -0
  36. regrender-0.1.0/regrender/_core.py +306 -0
  37. regrender-0.1.0/regrender/main_probe.py +702 -0
  38. regrender-0.1.0/regrender/main_register.py +638 -0
  39. regrender-0.1.0/regrender/main_roi.py +489 -0
  40. regrender-0.1.0/tests/test_core.py +167 -0
  41. regrender-0.1.0/tests/test_probe.py +24 -0
  42. regrender-0.1.0/tests/test_roi.py +74 -0
  43. regrender-0.1.0/uv.lock +4632 -0
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+ name: CI
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+
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+ on:
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+ push:
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+ branches: [main]
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+ pull_request:
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+
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+ jobs:
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+ lint:
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+ runs-on: ubuntu-latest
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+ steps:
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+ - uses: actions/checkout@v4
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+ - uses: astral-sh/setup-uv@v5
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+ - run: uvx ruff check .
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+ - run: uvx mypy regrender/
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+
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+ test:
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+ runs-on: ubuntu-latest
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+ steps:
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+ - uses: actions/checkout@v4
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+ - uses: astral-sh/setup-uv@v5
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+ with:
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+ python-version: "3.12"
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+ # napari import needs a display + Qt/OpenGL system libs
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+ - uses: pyvista/setup-headless-display-action@v3
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+ - run: uv sync --extra test
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+ - run: uv run pytest -q
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+ env:
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+ QT_QPA_PLATFORM: offscreen
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+ name: Publish to PyPI
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+
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+ # Publishes on a version tag push (e.g. `git push origin v0.1.0`).
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+ # The tag must match the version in pyproject.toml (checked below).
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+ on:
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+ push:
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+ tags:
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+ - "v*"
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+
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+ jobs:
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+ publish:
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+ runs-on: ubuntu-latest
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+ environment: pypi
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+ permissions:
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+ id-token: write # trusted publishing (OIDC) — no API token needed
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+ steps:
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+ - uses: actions/checkout@v4
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+ - uses: astral-sh/setup-uv@v5
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+ # fail early if the release tag (e.g. v0.1.0) doesn't match pyproject's version
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+ - name: Check tag matches version
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+ run: |
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+ tag="${GITHUB_REF_NAME#v}"
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+ pkg=$(grep '^version = ' pyproject.toml | cut -d'"' -f2)
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+ echo "tag=$tag pkg=$pkg"
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+ [ "$tag" = "$pkg" ] || { echo "tag $tag != pyproject version $pkg"; exit 1; }
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+ - run: uv build
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+ - uses: pypa/gh-action-pypi-publish@release/v1
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+ # Byte-compiled / optimized / DLL files
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+ __pycache__/
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+ *.py[cod]
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+ *$py.class
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+
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+ # C extensions
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+ *.so
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+
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+ # Distribution / packaging
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+ .Python
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+ build/
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+ develop-eggs/
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+ dist/
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+ downloads/
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+ eggs/
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+ .eggs/
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+ lib/
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+ lib64/
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+ parts/
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+ sdist/
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+ var/
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+ wheels/
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+ share/python-wheels/
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+ *.egg-info/
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+ .installed.cfg
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+ *.egg
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+ MANIFEST
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+
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+ # PyInstaller
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+ # Usually these files are written by a python script from a template
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+ # before PyInstaller builds the exe, so as to inject date/other infos into it.
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+ *.manifest
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+ *.spec
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+
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+ # Installer logs
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+ pip-log.txt
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+ pip-delete-this-directory.txt
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+
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+ # Unit test / coverage reports
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+ htmlcov/
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+ .tox/
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+ .nox/
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+ .coverage
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+ .coverage.*
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+ .cache
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+ nosetests.xml
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+ coverage.xml
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+ *.cover
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+ *.py,cover
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+ .hypothesis/
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+ .pytest_cache/
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+ cover/
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+
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+ # Translations
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+ *.mo
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+ *.pot
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+
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+ # Django stuff:
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+ *.log
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+ local_settings.py
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+ db.sqlite3
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+ db.sqlite3-journal
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+
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+ # Flask stuff:
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+ instance/
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+ .webassets-cache
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+
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+ # Scrapy stuff:
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+ .scrapy
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+
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+ # Sphinx documentation
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+ docs/_build/
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+
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+ # PyBuilder
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+ .pybuilder/
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+ target/
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+
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+ # Jupyter Notebook
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+ .ipynb_checkpoints
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+
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+ # IPython
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+ profile_default/
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+ ipython_config.py
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+
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+ # pyenv
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+ # For a library or package, you might want to ignore these files since the code is
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+ # intended to run in multiple environments; otherwise, check them in:
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+ # .python-version
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+
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+ # pipenv
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+ # According to pypa/pipenv#598, it is recommended to include Pipfile.lock in version control.
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+ # However, in case of collaboration, if having platform-specific dependencies or dependencies
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+ # having no cross-platform support, pipenv may install dependencies that don't work, or not
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+ # install all needed dependencies.
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+ #Pipfile.lock
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+
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+ # UV
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+ # Similar to Pipfile.lock, it is generally recommended to include uv.lock in version control.
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+ # This is especially recommended for binary packages to ensure reproducibility, and is more
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+ # commonly ignored for libraries.
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+ #uv.lock
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+
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+ # poetry
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+ # Similar to Pipfile.lock, it is generally recommended to include poetry.lock in version control.
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+ # This is especially recommended for binary packages to ensure reproducibility, and is more
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+ # commonly ignored for libraries.
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+ # https://python-poetry.org/docs/basic-usage/#commit-your-poetrylock-file-to-version-control
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+ #poetry.lock
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+
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+ # pdm
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+ # Similar to Pipfile.lock, it is generally recommended to include pdm.lock in version control.
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+ #pdm.lock
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+ # pdm stores project-wide configurations in .pdm.toml, but it is recommended to not include it
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+ # in version control.
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+ # https://pdm.fming.dev/latest/usage/project/#working-with-version-control
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+ .pdm.toml
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+ .pdm-python
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+ .pdm-build/
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+
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+ # PEP 582; used by e.g. github.com/David-OConnor/pyflow and github.com/pdm-project/pdm
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+ __pypackages__/
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+
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+ # Celery stuff
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+ celerybeat-schedule
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+ celerybeat.pid
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+
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+ # SageMath parsed files
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+ *.sage.py
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+
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+ # Environments
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+ .env
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+ .venv
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+ env/
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+ venv/
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+ ENV/
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+ env.bak/
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+ venv.bak/
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+
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+ # Spyder project settings
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+ .spyderproject
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+ .spyproject
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+
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+ # Rope project settings
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+ .ropeproject
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+
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+ # mkdocs documentation
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+ /site
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+
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+ # mypy
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+ .mypy_cache/
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+ .dmypy.json
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+ dmypy.json
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+
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+ # Pyre type checker
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+ .pyre/
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+
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+ # pytype static type analyzer
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+ .pytype/
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+
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+ # Cython debug symbols
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+ cython_debug/
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+
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+ # PyCharm
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+ # JetBrains specific template is maintained in a separate JetBrains.gitignore that can
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+ # be found at https://github.com/github/gitignore/blob/main/Global/JetBrains.gitignore
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+ # and can be added to the global gitignore or merged into this file. For a more nuclear
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+ # option (not recommended) you can uncomment the following to ignore the entire idea folder.
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+ #.idea/
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+
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+ # Abstra
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+ # Abstra is an AI-powered process automation framework.
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+ # Ignore directories containing user credentials, local state, and settings.
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+ # Learn more at https://abstra.io/docs
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+ .abstra/
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+
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+ # Visual Studio Code
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+ # Visual Studio Code specific template is maintained in a separate VisualStudioCode.gitignore
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+ # that can be found at https://github.com/github/gitignore/blob/main/Global/VisualStudioCode.gitignore
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+ # and can be added to the global gitignore or merged into this file. However, if you prefer,
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+ # you could uncomment the following to ignore the enitre vscode folder
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+ # .vscode/
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+
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+ # Ruff stuff:
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+ .ruff_cache/
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+
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+ # PyPI configuration file
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+ .pypirc
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+
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+ # Cursor
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+ # Cursor is an AI-powered code editor. `.cursorignore` specifies files/directories to
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+ # exclude from AI features like autocomplete and code analysis. Recommended for sensitive data
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+ # refer to https://docs.cursor.com/context/ignore-files
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+ .cursorignore
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+ .cursorindexingignore
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+ .DS_Store
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+ # Default ignored files
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+ /shelf/
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+ /workspace.xml
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+ # Editor-based HTTP Client requests
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+ /httpRequests/
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+ # Datasource local storage ignored files
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+ /dataSources/
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+ /dataSources.local.xml
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+ <?xml version="1.0" encoding="UTF-8"?>
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+ <excludeFolder url="file://$MODULE_DIR$/.venv" />
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+ <component name="PublishConfigData" remoteFilesAllowedToDisappearOnAutoupload="false">
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+ <paths name="yuting@172.18.1.31:22 password">
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+ <mapping local="$PROJECT_DIR$/../neuralib2" web="/" />
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+ <mapping local="$PROJECT_DIR$" web="/" />
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+ </paths>
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+ <component name="ProjectDictionaryState">
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+ <words>
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+ <w>allenccf</w>
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+ <profile version="1.0">
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+ <option name="myName" value="Project Default" />
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+ <inspection_tool class="DuplicatedCode" enabled="false" level="WEAK WARNING" enabled_by_default="false" />
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+ <inspection_tool class="Eslint" enabled="true" level="WARNING" enabled_by_default="true" />
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+ <inspection_tool class="PyInterpreterInspection" enabled="false" level="WARNING" enabled_by_default="false" />
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+ <component name="InspectionProjectProfileManager">
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+ <option name="USE_PROJECT_PROFILE" value="false" />
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+ <option name="sdkName" value="uv (pycams)" />
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+ </project>
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+ <project version="4">
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+ 3.12
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+ version: "2"
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+
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+ build:
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+ os: "ubuntu-24.04"
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+ tools:
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+ python: "3.12"
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+
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+ python:
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+ install:
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+ - method: pip
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+ path: .
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+ extra_requirements:
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+ - doc
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+
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+ sphinx:
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+ configuration: docs/source/conf.py
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+ MIT License
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+
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+ Copyright (c) 2026 Yu-Ting Wei
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+
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+ Permission is hereby granted, free of charge, to any person obtaining a copy
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+ of this software and associated documentation files (the "Software"), to deal
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+ in the Software without restriction, including without limitation the rights
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+ to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
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+ copies of the Software, and to permit persons to whom the Software is
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+ furnished to do so, subject to the following conditions:
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+
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+ The above copyright notice and this permission notice shall be included in all
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+ copies or substantial portions of the Software.
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+
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+ THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
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+ IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
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+ FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
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+ AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
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+ LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
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+ OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
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+ SOFTWARE.
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+ Metadata-Version: 2.4
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+ Name: regrender
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+ Version: 0.1.0
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+ Summary: 2D mouse brain registration, ROI/Probe annotation, and brainrender reconstruction
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+ Project-URL: Homepage, https://github.com/ytsimon2004/regrender
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+ Project-URL: Repository, https://github.com/ytsimon2004/regrender
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+ Project-URL: Documentation, https://regrender.readthedocs.io
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+ Author-email: Yu-Ting Wei <ytsimon2004@gmail.com>
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+ License-Expression: MIT
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+ License-File: LICENSE
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+ Keywords: allen-ccf,brain-atlas,brainrender,napari,neuroscience,registration
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+ Classifier: Intended Audience :: Science/Research
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+ Classifier: Operating System :: OS Independent
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+ Classifier: Programming Language :: Python :: 3.12
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+ Classifier: Topic :: Scientific/Engineering :: Bio-Informatics
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+ Requires-Python: >=3.12
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+ Requires-Dist: argclz>=1.0.1
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+ Requires-Dist: imageio
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+ Requires-Dist: napari[pyqt5]
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+ Requires-Dist: neuralib-atlas[brainrender]>=0.7.3
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+ Requires-Dist: rich
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+ Provides-Extra: doc
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+ Requires-Dist: pydata-sphinx-theme; extra == 'doc'
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+ Requires-Dist: sphinx; extra == 'doc'
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+ Requires-Dist: sphinx-copybutton; extra == 'doc'
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+ Provides-Extra: lint
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+ Requires-Dist: mypy; extra == 'lint'
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+ Requires-Dist: ruff; extra == 'lint'
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+ Provides-Extra: test
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+ Requires-Dist: pytest; extra == 'test'
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+ Description-Content-Type: text/markdown
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+
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+ # regrender
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+
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+ **Tools for 2D mouse brain registration to the Allen Common Coordinate Framework (CCF)**
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+
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+ A napari-based Python toolkit
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+ For registering histological brain slices to the Allen Brain Atlas, annotating cells/ROIs,
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+ reconstructing probe tracks, and rendering the results with brainrender.
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+
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+ ## Features
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+
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+ - **Interactive slice→CCF registration** (`regrender register`) — pick landmark pairs in napari, estimate a homography/affine transform.
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+ - **ROI annotation** (`regrender roi`) — label cells on raw images, project them into CCF space, and render.
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+ - **Probe-track reconstruction** (`regrender probe`) — reconstruct electrode shanks from dye labels across serial sections.
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+ - **brainrender rendering** of ROIs and probes in 3D atlas space.
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+
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+ ## Demo
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+
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+ **`regrender register`** — match landmark pairs between the atlas plane (left) and histology (right):
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+
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+ <img src="docs/source/_static/register.png" alt="register" width="800">
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+
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+ **`regrender roi`** — label cells per channel, then project + render in 3D with brainrender:
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+
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+ <img src="docs/source/_static/roi.png" alt="roi" width="800">
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+
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+ **`regrender probe`** — pick dye points per shank and reconstruct the tracks in 3D:
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+
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+ <img src="docs/source/_static/probe.png" alt="probe" width="800">
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+
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+
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+ ## Installation
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+
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+ ```bash
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+ # Option 1 — one-shot CLI install, no clone or env needed (recommended for users)
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+ uv tool install git+https://github.com/ytsimon2004/regrender.git
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+
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+ # Option 2 — uv virtual environment (development)
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+ git clone https://github.com/ytsimon2004/regrender.git && cd regrender
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+ uv venv && source .venv/bin/activate
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+ uv pip install -e .
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+
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+ # Option 3 — conda environment (development)
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+ git clone https://github.com/ytsimon2004/regrender.git && cd regrender
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+ conda create -n regrender python=3.12 -y && conda activate regrender
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+ pip install -e .
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+ ```
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+
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+ See the [installation docs](https://regrender.readthedocs.io/en/latest/get_start/installation.html) for details.
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+
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+ ## Quick Start
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+
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+ ```bash
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+ # 1. label ROIs on raw slices
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+ regrender roi -D <slices_dir>
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+
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+ # 2. register each slice to the CCF
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+ regrender register -D <slices_dir>
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+
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+ # 3. project ROIs to CCF + render (or use the GUI "Project + Render" button)
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+ regrender roi --project --render -D <slices_dir>
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+
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+ # probe tracks
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+ regrender probe -D <slices_dir>
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+ ```
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+
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+ See the [full documentation](https://regrender.readthedocs.io) for the complete workflow.
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+
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+ ## Contact
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+
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+ **Yu-Ting Wei** - ytsimon2004@gmail.com
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+ # regrender
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+
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+ **Tools for 2D mouse brain registration to the Allen Common Coordinate Framework (CCF)**
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+
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+ A napari-based Python toolkit
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+ For registering histological brain slices to the Allen Brain Atlas, annotating cells/ROIs,
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+ reconstructing probe tracks, and rendering the results with brainrender.
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+
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+ ## Features
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+
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+ - **Interactive slice→CCF registration** (`regrender register`) — pick landmark pairs in napari, estimate a homography/affine transform.
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+ - **ROI annotation** (`regrender roi`) — label cells on raw images, project them into CCF space, and render.
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+ - **Probe-track reconstruction** (`regrender probe`) — reconstruct electrode shanks from dye labels across serial sections.
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+ - **brainrender rendering** of ROIs and probes in 3D atlas space.
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+
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+ ## Demo
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+
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+ **`regrender register`** — match landmark pairs between the atlas plane (left) and histology (right):
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+
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+ <img src="docs/source/_static/register.png" alt="register" width="800">
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+
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+ **`regrender roi`** — label cells per channel, then project + render in 3D with brainrender:
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+
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+ <img src="docs/source/_static/roi.png" alt="roi" width="800">
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+
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+ **`regrender probe`** — pick dye points per shank and reconstruct the tracks in 3D:
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+
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+ <img src="docs/source/_static/probe.png" alt="probe" width="800">
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+
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+
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+ ## Installation
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+
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+ ```bash
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+ # Option 1 — one-shot CLI install, no clone or env needed (recommended for users)
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+ uv tool install git+https://github.com/ytsimon2004/regrender.git
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+
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+ # Option 2 — uv virtual environment (development)
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+ git clone https://github.com/ytsimon2004/regrender.git && cd regrender
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+ uv venv && source .venv/bin/activate
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+ uv pip install -e .
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+
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+ # Option 3 — conda environment (development)
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+ git clone https://github.com/ytsimon2004/regrender.git && cd regrender
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+ conda create -n regrender python=3.12 -y && conda activate regrender
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+ pip install -e .
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+ ```
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+
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+ See the [installation docs](https://regrender.readthedocs.io/en/latest/get_start/installation.html) for details.
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+
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+ ## Quick Start
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+
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+ ```bash
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+ # 1. label ROIs on raw slices
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+ regrender roi -D <slices_dir>
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+
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+ # 2. register each slice to the CCF
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+ regrender register -D <slices_dir>
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+
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+ # 3. project ROIs to CCF + render (or use the GUI "Project + Render" button)
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+ regrender roi --project --render -D <slices_dir>
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+
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+ # probe tracks
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+ regrender probe -D <slices_dir>
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+ ```
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+
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+ See the [full documentation](https://regrender.readthedocs.io) for the complete workflow.
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+
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+ ## Contact
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+
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+ **Yu-Ting Wei** - ytsimon2004@gmail.com
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+ # Minimal makefile for Sphinx documentation
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+ #
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+
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+ # You can set these variables from the command line, and also
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+ # from the environment for the first two.
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+ SPHINXOPTS ?=
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+ SPHINXBUILD ?= sphinx-build
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+ SOURCEDIR = source
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+ BUILDDIR = build
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+
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+ # Put it first so that "make" without argument is like "make help".
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+ help:
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+ @$(SPHINXBUILD) -M help "$(SOURCEDIR)" "$(BUILDDIR)" $(SPHINXOPTS) $(O)
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+
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+ .PHONY: help Makefile
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+
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+ # Catch-all target: route all unknown targets to Sphinx using the new
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+ # "make mode" option. $(O) is meant as a shortcut for $(SPHINXOPTS).
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+ %: Makefile
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+ @$(SPHINXBUILD) -M $@ "$(SOURCEDIR)" "$(BUILDDIR)" $(SPHINXOPTS) $(O)
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+ @ECHO OFF
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+
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+ pushd %~dp0
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+
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+ REM Command file for Sphinx documentation
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+
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+ if "%SPHINXBUILD%" == "" (
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+ set SPHINXBUILD=sphinx-build
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+ )
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+ set SOURCEDIR=source
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+ set BUILDDIR=build
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+
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+ %SPHINXBUILD% >NUL 2>NUL
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+ if errorlevel 9009 (
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+ echo.
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+ echo.The 'sphinx-build' command was not found. Make sure you have Sphinx
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+ echo.installed, then set the SPHINXBUILD environment variable to point
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+ echo.to the full path of the 'sphinx-build' executable. Alternatively you
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+ echo.may add the Sphinx directory to PATH.
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+ echo.
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+ echo.If you don't have Sphinx installed, grab it from
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+ echo.https://www.sphinx-doc.org/
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+ exit /b 1
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+ )
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+
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+ if "%1" == "" goto help
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+
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+ %SPHINXBUILD% -M %1 %SOURCEDIR% %BUILDDIR% %SPHINXOPTS% %O%
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+ goto end
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+
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+ :help
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+ %SPHINXBUILD% -M help %SOURCEDIR% %BUILDDIR% %SPHINXOPTS% %O%
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+
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+ :end
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+ popd