opencloning 0.2.8.1__tar.gz → 0.2.8.2__tar.gz

{opencloning-0.2.8.1 → opencloning-0.2.8.2}/PKG-INFO

@@ -1,6 +1,6 @@
 Metadata-Version: 2.3
 Name: opencloning
-Version: 0.2.8.1
+Version: 0.2.8.2
 Summary: Backend of OpenCloning, a web application to generate molecular cloning strategies in json format, and share them with others.
 License: MIT
 Author: Manuel Lera-Ramirez

{opencloning-0.2.8.1 → opencloning-0.2.8.2}/pyproject.toml

@@ -3,7 +3,7 @@ authors = ["Manuel Lera-Ramirez <manulera14@gmail.com>"]
 description = "Backend of OpenCloning, a web application to generate molecular cloning strategies in json format, and share them with others."
 license = "MIT"
 name = "opencloning"
-version = "v0.2.8.1"
+version = "v0.2.8.2"
 package-mode = true
 readme = "README.md"
 repository = "https://github.com/manulera/OpenCloning_backend"

{opencloning-0.2.8.1 → opencloning-0.2.8.2}/src/opencloning/dna_functions.py

@@ -241,16 +241,23 @@ def find_sequence_regex(pattern: str, seq: str, is_circular: bool) -> list[Locat
 def oligonucleotide_hybridization_overhangs(
     fwd_oligo_seq: str, rvs_oligo_seq: str, minimal_annealing: int
 ) -> list[int]:
+    """
+    Returns possible overhangs between two oligos, and returns an error if mismatches are found.
+
+    see https://github.com/manulera/OpenCloning_backend/issues/302 for notation
+
+    """
     matches = common_sub_strings(fwd_oligo_seq.lower(), reverse_complement(rvs_oligo_seq.lower()), minimal_annealing)
 
-    for m in matches:
-        if not (
-            (m[0] == 0 and m[1] + m[2] == len(fwd_oligo_seq)) or (m[1] == 0 and m[0] + m[2] == len(rvs_oligo_seq))
+    for pos_fwd, pos_rvs, length in matches:
+
+        if (pos_fwd != 0 and pos_rvs != 0) or (
+            pos_fwd + length < len(fwd_oligo_seq) and pos_rvs + length < len(rvs_oligo_seq)
         ):
             raise ValueError('The oligonucleotides can anneal with mismatches')
 
     # Return possible overhangs
-    return [start_on_rvs - start_on_fwd for start_on_fwd, start_on_rvs, length in matches]
+    return [pos_rvs - pos_fwd for pos_fwd, pos_rvs, length in matches]
 
 
 class MyGenBankScanner(GenBankScanner):