microlive 1.0.19__tar.gz → 1.0.21__tar.gz

{microlive-1.0.19 → microlive-1.0.21}/PKG-INFO

@@ -1,6 +1,6 @@
 Metadata-Version: 2.4
 Name: microlive
-Version: 1.0.19
+Version: 1.0.21
 Summary: Live-cell microscopy image analysis and single-molecule measurements
 Project-URL: Homepage, https://github.com/ningzhaoAnschutz/microlive
 Project-URL: Documentation, https://github.com/ningzhaoAnschutz/microlive/blob/main/docs/user_guide.md

{microlive-1.0.19 → microlive-1.0.21}/microlive/__init__.py

@@ -23,7 +23,7 @@ Authors:
     Nathan L. Nowling, Brian Munsky, Ning Zhao
 """
 
-__version__ = "1.0.19"
+__version__ = "1.0.21"
 __author__ = "Luis U. Aguilera, William S. Raymond, Rhiannon M. Sears, Nathan L. Nowling, Brian Munsky, Ning Zhao"
 
 # Package name (for backward compatibility)

{microlive-1.0.19 → microlive-1.0.21}/microlive/gui/app.py

@@ -1946,6 +1946,9 @@ class GUI(QMainWindow):
              self.cellpose_masks_nuc = None
              if hasattr(self, 'manual_segmentation_mask'):
                  del self.manual_segmentation_mask
+             # Reset segmentation tab to Watershed (index 0) since Edit tab is only relevant after segmentation
+             if hasattr(self, 'segmentation_method_tabs'):
+                 self.segmentation_method_tabs.setCurrentIndex(0)
         
         self.plot_image()
         self.plot_tracking()
@@ -7859,9 +7862,12 @@ class GUI(QMainWindow):
                 z_spot_size_in_px=z_spot_size,
                 use_3d=use_3d
             )
-            threshold = auto_thresh.calculate()
+            threshold_raw = auto_thresh.calculate()
             method_used = auto_thresh.method_used
             
+            # Reduce threshold by 10% to improve spot coverage (auto-threshold tends to overestimate)
+            threshold = threshold_raw * 0.9
+            
             # Store per-channel
             self.auto_threshold_per_channel[channel] = threshold