microeye 2.3.13__tar.gz → 2.4.0a2__tar.gz

This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
Files changed (223) hide show
  1. {microeye-2.3.13/src/microeye.egg-info → microeye-2.4.0a2}/PKG-INFO +90 -80
  2. {microeye-2.3.13 → microeye-2.4.0a2}/README.md +86 -76
  3. microeye-2.4.0a2/src/microEye/_version.py +2 -0
  4. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/filters/base.py +2 -1
  5. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/multi_viewer.py +1 -1
  6. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/__init__.py +1 -2
  7. microeye-2.4.0a2/src/microEye/hardware/cams/basler/basler_cam.py +1014 -0
  8. microeye-2.4.0a2/src/microEye/hardware/cams/basler/basler_panel.py +394 -0
  9. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/camera_list.py +172 -160
  10. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/camera_options.py +17 -30
  11. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/camera_panel.py +204 -60
  12. microeye-2.4.0a2/src/microEye/hardware/cams/dummy/dummy_panel.py +184 -0
  13. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/micam.py +480 -38
  14. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/pco/pco_cam.py +95 -9
  15. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/pco/pco_panel.py +5 -169
  16. microeye-2.4.0a2/src/microEye/hardware/cams/pycromanager/__init__.py +1 -0
  17. microeye-2.4.0a2/src/microEye/hardware/cams/pycromanager/pycro_cam.py +194 -0
  18. {microeye-2.3.13/src/microEye/hardware/pycromanager/widgets → microeye-2.4.0a2/src/microEye/hardware/cams/pycromanager}/pycro_panel.py +19 -104
  19. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/thorlabs/thorlabs.py +141 -4
  20. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/thorlabs/thorlabs_panel.py +26 -233
  21. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/ueye/ueye_camera.py +148 -3
  22. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/ueye/ueye_panel.py +23 -218
  23. microeye-2.4.0a2/src/microEye/hardware/cams/vimba/__init__.py +7 -0
  24. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/vimba/vimba_cam.py +398 -66
  25. microeye-2.4.0a2/src/microEye/hardware/cams/vimba/vimba_panel.py +393 -0
  26. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/mieye/acquisition_manager.py +42 -19
  27. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/mieye/devices_manager.py +100 -226
  28. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/mieye/miEye.py +66 -65
  29. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/core.py +1 -0
  30. microeye-2.4.0a2/src/microEye/hardware/pycromanager/devices.py +214 -0
  31. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/widgets/__init__.py +0 -1
  32. microeye-2.4.0a2/src/microEye/hardware/stages/kinesis/__init__.py +1 -0
  33. microeye-2.4.0a2/src/microEye/hardware/stages/kinesis/kinesis.py +164 -0
  34. microeye-2.4.0a2/src/microEye/hardware/stages/manager.py +456 -0
  35. microeye-2.4.0a2/src/microEye/hardware/stages/piezon_concept/__init__.py +1 -0
  36. microeye-2.3.13/src/microEye/hardware/stages/piezo_concept.py → microeye-2.4.0a2/src/microEye/hardware/stages/piezon_concept/foc.py +22 -32
  37. microeye-2.4.0a2/src/microEye/hardware/stages/pycromanager/__init__.py +1 -0
  38. microeye-2.4.0a2/src/microEye/hardware/stages/pycromanager/core.py +232 -0
  39. microeye-2.4.0a2/src/microEye/hardware/stages/smaract/__init__.py +1 -0
  40. microeye-2.4.0a2/src/microEye/hardware/stages/smaract/mcs2.py +123 -0
  41. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/stabilizer.py +1 -1
  42. microeye-2.4.0a2/src/microEye/hardware/stages/stage.py +870 -0
  43. microeye-2.4.0a2/src/microEye/hardware/stages/view.py +409 -0
  44. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/widgets/controller.py +46 -80
  45. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/widgets/devices.py +0 -29
  46. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/widgets/scan_acquisition.py +9 -4
  47. microeye-2.4.0a2/src/microEye/hardware/widgets/slides.py +550 -0
  48. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/launcher.py +7 -9
  49. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/micro_launcher.py +10 -6
  50. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/parameter_tree.py +2 -2
  51. {microeye-2.3.13 → microeye-2.4.0a2/src/microeye.egg-info}/PKG-INFO +90 -80
  52. {microeye-2.3.13 → microeye-2.4.0a2}/src/microeye.egg-info/SOURCES.txt +13 -2
  53. {microeye-2.3.13 → microeye-2.4.0a2}/src/microeye.egg-info/requires.txt +3 -3
  54. microeye-2.3.13/src/microEye/_version.py +0 -2
  55. microeye-2.3.13/src/microEye/hardware/cams/basler/basler_cam.py +0 -413
  56. microeye-2.3.13/src/microEye/hardware/cams/dummy/dummy_panel.py +0 -695
  57. microeye-2.3.13/src/microEye/hardware/cams/vimba/vimba_panel.py +0 -745
  58. microeye-2.3.13/src/microEye/hardware/lasers/__init__.py +0 -1
  59. microeye-2.3.13/src/microEye/hardware/pycromanager/devices.py +0 -487
  60. microeye-2.3.13/src/microEye/hardware/stages/kinesis/__init__.py +0 -1
  61. microeye-2.3.13/src/microEye/hardware/stages/kinesis/kinesis.py +0 -771
  62. microeye-2.3.13/src/microEye/hardware/stages/stage.py +0 -851
  63. {microeye-2.3.13 → microeye-2.4.0a2}/LICENSE +0 -0
  64. {microeye-2.3.13 → microeye-2.4.0a2}/MANIFEST.in +0 -0
  65. {microeye-2.3.13 → microeye-2.4.0a2}/pyproject.toml +0 -0
  66. {microeye-2.3.13 → microeye-2.4.0a2}/setup.cfg +0 -0
  67. {microeye-2.3.13 → microeye-2.4.0a2}/setup.py +0 -0
  68. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/__init__.py +0 -0
  69. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/__init__.py +0 -0
  70. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/checklist_dialog.py +0 -0
  71. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/cmosMaps.py +0 -0
  72. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/filters/__init__.py +0 -0
  73. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/filters/spatial.py +0 -0
  74. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/filters/temporal.py +0 -0
  75. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/__init__.py +0 -0
  76. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/fit.py +0 -0
  77. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/nena.py +0 -0
  78. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/phasor_fit.py +0 -0
  79. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/processing.py +0 -0
  80. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/__init__.py +0 -0
  81. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/extract.py +0 -0
  82. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/rubost_mean.py +0 -0
  83. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/spline.py +0 -0
  84. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/stats/__init__.py +0 -0
  85. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/stats/core.py +0 -0
  86. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/stats/curve_fit.py +0 -0
  87. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/stats/io.py +0 -0
  88. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/stats/slope_fit.py +0 -0
  89. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/temp.py +0 -0
  90. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/test.py +0 -0
  91. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/CPU/CPUfunctions.py +0 -0
  92. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/CPU/CPUmleFit_LM.py +0 -0
  93. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/CPU/CPUsplineLib.py +0 -0
  94. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/CPU/__init__.py +0 -0
  95. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/GPU/GPUfunctions.py +0 -0
  96. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/GPU/GPUmleFit_LM_EMCCD.py +0 -0
  97. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/GPU/GPUmleFit_LM_sCMOS.py +0 -0
  98. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/GPU/GPUsplineLib.py +0 -0
  99. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/GPU/__init__.py +0 -0
  100. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/__init__.py +0 -0
  101. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/constants.py +0 -0
  102. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/mainfunctions.py +0 -0
  103. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/rcc.py +0 -0
  104. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/results.py +0 -0
  105. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/results_stats.py +0 -0
  106. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/tardis.py +0 -0
  107. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/processing/__init__.py +0 -0
  108. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/processing/frc.py +0 -0
  109. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/rendering/__init__.py +0 -0
  110. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/rendering/base.py +0 -0
  111. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/rendering/cloud.py +0 -0
  112. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/rendering/core.py +0 -0
  113. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/rendering/volumetric.py +0 -0
  114. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/tools/__init__.py +0 -0
  115. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/tools/kymograms.py +0 -0
  116. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/tools/roi_selectors.py +0 -0
  117. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/utils/__init__.py +0 -0
  118. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/utils/coordinates.py +0 -0
  119. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/utils/images.py +0 -0
  120. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/utils/windows.py +0 -0
  121. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/__init__.py +0 -0
  122. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/image_options_widget.py +0 -0
  123. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/images.py +0 -0
  124. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/layers_widget.py +0 -0
  125. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/localizations.py +0 -0
  126. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/psf.py +0 -0
  127. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/volume.py +0 -0
  128. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/__init__.py +0 -0
  129. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/basler/__init__.py +0 -0
  130. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/camera_calibration.py +0 -0
  131. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/dummy/__init__.py +0 -0
  132. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/jobs.py +0 -0
  133. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/line_profiler.py +0 -0
  134. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/linescan/IR_Cam.py +0 -0
  135. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/linescan/__init__.py +0 -0
  136. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/pco/__init__.py +0 -0
  137. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/pco/enums.py +0 -0
  138. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/shortcuts.py +0 -0
  139. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/thorlabs/__init__.py +0 -0
  140. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/ueye/__init__.py +0 -0
  141. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/device.py +0 -0
  142. {microeye-2.3.13/src/microEye/hardware/cams/vimba → microeye-2.4.0a2/src/microEye/hardware/lasers}/__init__.py +0 -0
  143. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/lasers/io_matchbox.py +0 -0
  144. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/lasers/io_params.py +0 -0
  145. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/lasers/io_single_laser.py +0 -0
  146. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/lasers/laser_relay.py +0 -0
  147. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/mieye/__init__.py +0 -0
  148. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/misc/__init__.py +0 -0
  149. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/misc/acquisition_view.py +0 -0
  150. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/misc/reglo.py +0 -0
  151. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/misc/temp.py +0 -0
  152. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/port_config.py +0 -0
  153. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/protocols/__init__.py +0 -0
  154. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/protocols/actions.py +0 -0
  155. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/protocols/actions_items.py +0 -0
  156. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/protocols/designer.py +0 -0
  157. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/protocols/scene_manager.py +0 -0
  158. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/protocols/serialization.py +0 -0
  159. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/__init__.py +0 -0
  160. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/enums.py +0 -0
  161. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/headless.py +0 -0
  162. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/utils.py +0 -0
  163. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/widgets/bridges.py +0 -0
  164. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/widgets/headless_manager.py +0 -0
  165. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/widgets/headless_options.py +0 -0
  166. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/__init__.py +0 -0
  167. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/__init__.py +0 -0
  168. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/baseDevice.py +0 -0
  169. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/device.py +0 -0
  170. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/deviceID.py +0 -0
  171. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/devicePort.py +0 -0
  172. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/deviceStatus.py +0 -0
  173. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/devicesView.py +0 -0
  174. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/ellDevices.py +0 -0
  175. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/messageUpdater.py +0 -0
  176. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/motorInfo.py +0 -0
  177. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/stage.py +0 -0
  178. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/test.py +0 -0
  179. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/kinesis/kdc101/__init__.py +0 -0
  180. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/kinesis/kdc101/enums.py +0 -0
  181. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/kinesis/kdc101/factory.py +0 -0
  182. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/kinesis/kdc101/kdc101.py +0 -0
  183. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/widgets/__init__.py +0 -0
  184. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/widgets/focusWidget.py +0 -0
  185. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/widgets/qlist_slider.py +0 -0
  186. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/1024.png +0 -0
  187. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/128.png +0 -0
  188. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/16.png +0 -0
  189. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/24.png +0 -0
  190. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/256.png +0 -0
  191. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/32.png +0 -0
  192. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/48.png +0 -0
  193. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/512.png +0 -0
  194. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/64.png +0 -0
  195. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/__init__.py +0 -0
  196. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/close.svg +0 -0
  197. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/mieye.png +0 -0
  198. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/min.svg +0 -0
  199. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/viewer.png +0 -0
  200. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/qt.py +0 -0
  201. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/tools/__init__.py +0 -0
  202. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/tools/microscopy.py +0 -0
  203. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/__init__.py +0 -0
  204. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/display.py +0 -0
  205. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/enum_encoder.py +0 -0
  206. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/expandable_groupbox.py +0 -0
  207. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/gui_helper.py +0 -0
  208. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/hid_utils/__init__.py +0 -0
  209. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/hid_utils/controller.py +0 -0
  210. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/hid_utils/device.py +0 -0
  211. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/hid_utils/enums.py +0 -0
  212. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/hid_utils/utils.py +0 -0
  213. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/labelled_slider.py +0 -0
  214. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/metadata.py +0 -0
  215. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/metadata_tree.py +0 -0
  216. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/pyscripting.py +0 -0
  217. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/retry_exec.py +0 -0
  218. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/start_gui.py +0 -0
  219. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/thread_worker.py +0 -0
  220. {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/uImage.py +0 -0
  221. {microeye-2.3.13 → microeye-2.4.0a2}/src/microeye.egg-info/dependency_links.txt +0 -0
  222. {microeye-2.3.13 → microeye-2.4.0a2}/src/microeye.egg-info/entry_points.txt +0 -0
  223. {microeye-2.3.13 → microeye-2.4.0a2}/src/microeye.egg-info/top_level.txt +0 -0
@@ -1,6 +1,6 @@
1
1
  Metadata-Version: 2.4
2
2
  Name: microeye
3
- Version: 2.3.13
3
+ Version: 2.4.0a2
4
4
  Summary: A python toolkit for fluorescence microscopy that features hardware control, data analysis and vizualization for super-resolution single-molecule localization microscopy and single-partical tracking.
5
5
  Home-page: https://github.com/samhitech/microEye
6
6
  Author: Mohammad Nour Alsamsam
@@ -33,7 +33,7 @@ Requires-Dist: pyflakes==3.2.0
33
33
  Requires-Dist: pyjokes==0.6.0
34
34
  Requires-Dist: PyOpenGL==3.1.7
35
35
  Requires-Dist: pypylon==4.2.0
36
- Requires-Dist: pyqtdarktheme==2.1.0
36
+ Requires-Dist: pyqtdarktheme>=0.1.7
37
37
  Requires-Dist: pyqtgraph==0.13.7
38
38
  Requires-Dist: pyserial==3.5
39
39
  Requires-Dist: PySide6==6.7.2
@@ -44,8 +44,8 @@ Requires-Dist: QDarkStyle==3.2.3
44
44
  Requires-Dist: QScintilla==2.14.1
45
45
  Requires-Dist: scikit-image==0.22.0
46
46
  Requires-Dist: scikit_learn==1.5.0
47
- Requires-Dist: scipy==1.14.1
48
- Requires-Dist: setuptools==69.0.2
47
+ Requires-Dist: scipy>=1.13.1
48
+ Requires-Dist: setuptools>=80.9.0
49
49
  Requires-Dist: tables==3.9.1
50
50
  Requires-Dist: tabulate==0.9.0
51
51
  Requires-Dist: tqdm==4.66.4
@@ -72,11 +72,16 @@ The **`microEye`** is a Python toolkit for fluorescence microscopy that supports
72
72
  This toolkit is compatible with the [hardware](#hardware) used in our microscope. For further details, refer to the [miEye microscope paper](https://doi.org/10.1016/j.ohx.2022.e00368) and [OSF project](http://doi.org/10.17605/osf.io/j2fqy).
73
73
 
74
74
  ```bash
75
- __ ____ ____ ___ ____ _______
76
- / |/ (_)__________ / __/_ _____ _ __|_ | |_ / < /_ /
77
- / /|_/ / / __/ __/ _ \/ _// // / -_) | |/ / __/_ _/_ <_ / //_ <
78
- /_/ /_/_/\__/_/ \___/___/\_, /\__/ |___/____(_)____(_)_/____/
75
+ __ ____ ____ ___ ____ ___ ___
76
+ / |/ (_)__________ / __/_ _____ _ __|_ |/ / / / _ \___ _|_ |
77
+ / /|_/ / / __/ __/ _ \/ _// // / -_) | |/ / __//_ _// // / _ `/ __/
78
+ /_/ /_/_/\__/_/ \___/___/\_, /\__/ |___/____(_)_/(_)___/\_,_/____/
79
79
  /___/
80
+ ___ __ __ ___ __
81
+ / _ | / /__ / / ___ _ / _ \___ / /__ ___ ____ ___
82
+ / __ |/ / _ \/ _ \/ _ `/ / , _/ -_) / -_) _ `(_-</ -_)
83
+ /_/ |_/_/ .__/_//_/\_,_/ /_/|_|\__/_/\__/\_,_/___/\__/
84
+ /_/
80
85
  ```
81
86
 
82
87
  ![Package Health](https://snyk.io/advisor/python/microEye/badge.svg)
@@ -124,6 +129,7 @@ This toolkit is compatible with the [hardware](#hardware) used in our microscope
124
129
  - [Features](#features)
125
130
  - [Integrated Hardware](#integrated-hardware)
126
131
  - [How to Use](#how-to-use)
132
+ - [Step-by-step Instructions](#step-by-step-instructions)
127
133
  - [Authors](#authors)
128
134
  - [People Involved](#people-involved)
129
135
  - [Acknowledgement](#acknowledgement)
@@ -133,7 +139,7 @@ This toolkit is compatible with the [hardware](#hardware) used in our microscope
133
139
 
134
140
  1. **Install Python:**
135
141
 
136
- Download and install the latest [Python](https://www.python.org/downloads/) ≥3.9 stable release.
142
+ Download and install the latest [`Python`](https://www.python.org/downloads/) stable release (version ≥3.9 and ≤3.11).
137
143
 
138
144
  2. **Install microEye package:**
139
145
 
@@ -149,31 +155,27 @@ This toolkit is compatible with the [hardware](#hardware) used in our microscope
149
155
  pip install microEye==version
150
156
  ```
151
157
 
152
- 3. **Install required packages: (Optional)**
158
+ 3. **Install specific hardware drivers: (Optional)**
153
159
 
154
- Download the [requirements.txt](https://github.com/samhitech/microEye/blob/main/requirements.txt) file. Navigate to the directory containing the requirements file in your terminal and run:
160
+ - Allied Vision CMOS cameras: Install the [`Vimba X SDK`](https://www.alliedvision.com/en/products/software/vimba-x-sdk/) (_avoid installing it inside the Program Files directory_). After installation, navigate to the `Python API` directory (e.g., `C:\Allied Vision\Vimba X\api\python`) where the `vmbpy` wheel file (`.whl`) is located, and install it using:
155
161
 
156
- ```powershell
157
- pip install -r requirements.txt
158
- ```
159
-
160
- Note: This step is optional as dependecies are installed with the package.
162
+ ```bash
163
+ python -m pip install vmbpy-1.1.1-py3-none-win_amd64.whl
164
+ ```
161
165
 
162
- 4. **Install specific hardware drivers: (Optional)**
166
+ - Basler CMOS cameras: Install [`pylon`](https://www.baslerweb.com/en/downloads/software/?srsltid=AfmBOoqsMrbQT24hcWiCw-0ptD9PR7nCrPMBZSzi0YlI1CVItNQikMKW&downloadCategory.values.label.data=pylon) (_avoid installing it inside the Program Files directory_).
167
+ - IDS uEye CMOS cameras: Install [`IDS Software Suite 4.96.1`](https://en.ids-imaging.com/download-details/AB00604.html?os=windows&version=win10&bus=64&floatcalc=) for Windows 32/64-bit.
168
+ - Integrated Optics Lasers: Install [`Laser control software`](https://integratedoptics.com/downloads).
163
169
 
164
- - For Integrated Optics: Download and install [Laser control software](https://integratedoptics.com/downloads).
165
- - For IDS uEye CMOS cameras: Install [IDS Software Suite 4.96.1](https://en.ids-imaging.com/download-details/AB00604.html?os=windows&version=win10&bus=64&floatcalc=) for Windows 32/64-bit.
166
- - For Allied Vision CMOS cameras: Install [Vimba SDK](https://www.alliedvision.com/en/products/vimba-sdk) 5.0 or 6.0 outside the Program Files. Navigate to the directory containing setup.py and run:
170
+ - PCO cameras: Install [`pco.sdk`, `pco.runtime`](https://www.excelitas.com/product/pco-software-development-kits).
167
171
 
168
- ```powershell
169
- python -m pip install .
170
- ```
172
+ > **Note:** Support is under development.
171
173
 
172
- - For Thorlabs CMOS cameras: Install [Thorcam](https://www.thorlabs.com/software_pages/ViewSoftwarePage.cfm?Code=ThorCam) in its default directory. Note: Some Thorlabs cameras may be identified as IDS uEye cameras by Windows and may run without Thorcam.
174
+ - Thorlabs CMOS cameras: Install [`Thorcam`](https://www.thorlabs.com/software_pages/ViewSoftwarePage.cfm?Code=ThorCam) in its default directory. Note: Some Thorlabs cameras may be identified as IDS uEye cameras by Windows and may run without Thorcam.
173
175
 
174
- - For Thorlabs hardware, install [Kinesis® Software](https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=10285) and [Elliptec™ Software](https://www.thorlabs.com/software_pages/ViewSoftwarePage.cfm?Code=ELL).
176
+ - Thorlabs hardware, install [`Kinesis® Software`](https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=10285) and [`Elliptec™ Software`](https://www.thorlabs.com/software_pages/ViewSoftwarePage.cfm?Code=ELL).
175
177
 
176
- 5. **Open a terminal and execute microEye:** :partying_face:
178
+ 4. **Open a terminal and execute microEye:** :partying_face:
177
179
 
178
180
  ```powershell
179
181
  usage: microEye.exe [-h] [--module MODULE] [--QT_API QT_API] [--theme THEME]
@@ -194,12 +196,12 @@ This toolkit is compatible with the [hardware](#hardware) used in our microscope
194
196
 
195
197
  If you encounter any issues during the installation process, please check the following:
196
198
 
197
- - Ensure that you have the latest version of Python installed (≥3.9).
199
+ - Ensure that you have the latest version of Python installed (`3.9 ≤ version ≤3.11`).
198
200
  - Try installing the required packages repeating step (2) or individually using `pip install <package_name>`.
199
201
  - Check the system requirements for specific hardware drivers and follow the installation instructions provided by the manufacturers.
200
202
  - Refer to the project's issue tracker on GitHub for any known installation issues and solutions.
201
203
 
202
- If the issue persists, feel free to open a new issue on the project's GitHub repository, providing detailed information about the problem and any error messages you encountered.
204
+ > If the issue persists, feel free to open a new issue on the project's GitHub repository, providing detailed information about the problem and any error messages you encountered.
203
205
 
204
206
  ## microEye Launcher
205
207
 
@@ -213,10 +215,10 @@ Upon running the launcher, you will be presented with the following interface:
213
215
 
214
216
  - **miEye Module**: Launches the miEye module for microscope control and acquisition.
215
217
  - **Viewer Module**: Launches the viewer module for image/data anlysis and visualization.
216
- - **QT API (dropdown)**: Select the Qt API to use. Options are PySide6, PyQt6, or PyQt5.
217
- - **Theme (dropdown)**: Select the theme for the application. Options are None (default), qdarktheme, or qdarkstyle.
218
+ - **QT API (dropdown)**: Select the `Qt API` to use. Options are `PySide6`, `PyQt6`, or `PyQt5`.
219
+ - **Theme (dropdown)**: Select the `theme` for the application. Options are `None` (default), `qdarktheme`, or `qdarkstyle`.
218
220
 
219
- To launch a module, simply click on the respective button (`miEye Module` or `Viewer Module`). If you wish to change the Qt API or theme, select the desired option from the dropdown menus before launching.
221
+ To launch a module, simply click on the respective button (`miEye Module` or `Viewer Module`). If you wish to change the `Qt API` or `theme`, select the desired option from the dropdown menus before launching.
220
222
 
221
223
  ## Modules
222
224
 
@@ -258,7 +260,7 @@ Key features include:
258
260
  To access the Experiment Designer:
259
261
 
260
262
  1. Launch the `miEye module`.
261
- 2. Look for the Experiment Designer (Protocols) view in the interface.
263
+ 2. Look for the `Protocols` view in the interface.
262
264
 
263
265
  Note: This feature is currently in beta. We value your feedback to enhance its functionality and improve the user experience.
264
266
 
@@ -282,23 +284,25 @@ microEye --module viewer
282
284
 
283
285
  The `microEye` uses the following Python packages:
284
286
 
285
- | Data Analysis and Visualization | GUI and UI Development | Code Quality and Formatting | Image and Video Processing | File and Data Storage | Other Utilities | Microscopy |
286
- | :-----------------------------: | :--------------------: | :-------------------------: | :------------------------: | :-------------------: | :-------------: | :----------: |
287
- | dask | PySide6 | autopep8 | opencv-python | ome-types | hidapi | pycromanager |
288
- | matplotlib | pyqtdarktheme | pyflakes | | tables | pyfiglet | |
289
- | numba | pyqtgraph | | | zarr | pyserial | |
290
- | numpy | QDarkStyle | | | | pyjokes | |
291
- | pandas | QScintilla | | | | setuptools | |
292
- | scikit-image | PyQt5 (optional) | | | | tabulate | |
293
- | scikit_learn | PyQt6 (optional) | | | | pyueye | |
294
- | scipy | | | | | tqdm | |
295
- | tifffile | | | | | | |
296
- | h5py | | | | | | |
297
- | pco | | | | | | |
298
- | | | | | | | |
299
- | PyOpenGL | | | | | | |
300
-
301
- Note: VimbaPython is included in Vimba SDK and needs to be installed manually.
287
+ | Data Analysis and Visualization | GUI and UI Development | Code Quality and Formatting | Image and Video Processing | File and Data Storage | Other Utilities | Microscopy |
288
+ | :-----------------------------: | :-------------------------: | :-------------------------: | :------------------------: | :-------------------: | :-------------: | :----------: |
289
+ | dask | PySide6 | autopep8 | opencv-python | ome-types | hidapi | pycromanager |
290
+ | matplotlib | pyqtdarktheme | pyflakes | | tables | pyfiglet | |
291
+ | numba | pyqtgraph | | | zarr | pyserial | |
292
+ | numpy | QDarkStyle | | | | pyjokes | |
293
+ | pandas | QScintilla | | | | setuptools | |
294
+ | scikit-image | PyQt5 (optional) | | | | tabulate | |
295
+ | scikit_learn | PyQt6 (optional) | | | | pyueye | |
296
+ | scipy | PyQt6-QScintilla (optional) | | | | tqdm | |
297
+ | tifffile | | | | | | |
298
+ | h5py | | | | | | |
299
+ | pco | | | | | | |
300
+ | | | | | | | |
301
+ | PyOpenGL | | | | | | |
302
+
303
+ > **Note:** The VimbaPython package is bundled with the Vimba SDK and must be installed manually.
304
+ >
305
+ > **IMPORTANT:** Starting from microEye v2.4.0, the legacy Vimba SDK is no longer supported. Please use the Vimba X SDK and its accompanying _vmbpy_ Python package instead.
302
306
 
303
307
  ## Microscope Scheme
304
308
 
@@ -319,45 +323,47 @@ Schematic overview of the miEye instrument:
319
323
 
320
324
  ### Supported Cameras
321
325
 
322
- | Camera | Description | Link |
323
- | :-----------------------: | ------------------------------------------------------------------------ | :-----------------------------------------------------------------------------------------------------------: |
324
- | IDS uEye UI-3060CP Rev. 2 | IDS industrial-grade CMOS cameras | [Product Link](https://en.ids-imaging.com/store/products/cameras/ui-3060cp-rev-2.html) |
325
- | Thorlabs DCC1545M | DCx camera using UC480 driver | [Product Link](https://www.thorlabs.com/thorProduct.cfm?partNumber=DCC1545M) |
326
- | Allied Vision Alvium 1800 | Allied Vision industrial-grade CMOS cameras (U-158m, U-511m) | [Product Link](https://www.alliedvision.com/en/products/alvium-configurator/alvium-1800-u/158/#_configurator) |
327
- | Excelitas PCO sCMOS | Excelitas pco.edge 4.2 LT USB sCMOS Camera | [Product Link](https://www.excelitas.com/product/pcoedge-42-lt-usb-scmos-camera) |
328
- | Pycro-camera | Access to cameras via Micro-Manager drivers/adapters using Pycro-Manager | - |
326
+ | Camera | Description | Driver | Link |
327
+ | :-----------------------: | ------------------------------------------------------------------------ | :----------------------------: | :-----------------------------------------------------------------------------------------------------------: |
328
+ | IDS uEye UI-3060CP Rev. 2 | IDS industrial-grade CMOS cameras | `IDS uEye SDK 4.96.1` `pyueye` | [Product Link](https://en.ids-imaging.com/store/products/cameras/ui-3060cp-rev-2.html) |
329
+ | Thorlabs DCC1545M | DCx camera using UC480 driver | `ThorCam 3.7.0` | [Product Link](https://www.thorlabs.com/thorProduct.cfm?partNumber=DCC1545M) |
330
+ | Allied Vision Alvium 1800 | Allied Vision industrial-grade CMOS cameras (U-158m, U-511m) | `Vimba X SDK` `vmbpy` | [Product Link](https://www.alliedvision.com/en/products/alvium-configurator/alvium-1800-u/158/#_configurator) |
331
+ | Excelitas PCO sCMOS | Excelitas pco.edge 4.2 LT USB sCMOS Camera | `pco.sdk` `pco.python` | [Product Link](https://www.excelitas.com/product/pcoedge-42-lt-usb-scmos-camera) |
332
+ | Pycro-camera | Access to cameras via Micro-Manager drivers/adapters using Pycro-Manager | `uManager` `Pycro-Manager` | - |
329
333
 
330
334
  ### Additional Hardware
331
335
 
332
- | Hardware | Description | Link |
333
- | --------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ |
334
- | Integrated Optics MatchBox | Multi-wavelength Laser Combiner, Single Laser MatchBox | [Products Link](https://integratedoptics.com/products/wavelength-combiners) |
335
- | Piezo Concept FOC | Nanopositioner for microscope objectives | [Piezo Concept FOC](https://piezoconcept-store.squarespace.com/1-axis/p/foc) |
336
- | Thorlabs Elliptec ELL6/ELL9/ELL12 | Dual/Four/Six-Position Slider | [ELL6](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL6), [ELL9](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL9), [ELL12](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL12) |
337
- | Thorlabs Elliptec ELL14 | Rotation Mount: SM1 Threaded | [ELL14](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL14) |
338
- | Thorlabs Elliptec ELL20 | Linear Stage: 60 mm Travel | [ELL20](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL20) |
339
- | Thorlabs KDC101 | Kinesis Controller for Z825B/[Z925B](https://www.thorlabs.com/thorproduct.cfm?partnumber=Z925B) actuators (Activate USB VCP to access the COM port in device manager) | [KDC101](https://www.thorlabs.com/thorproduct.cfm?partnumber=KDC101) |
340
- | Parallax TSL1401-DB (#28317) | Linescan Camera Module | [Parallax TSL1401-DB](https://eu.mouser.com/ProductDetail/Parallax/28317?qs=%2Fha2pyFaduiCRhuOAXMuCmQIeG1Q3R01m6Y1EH%252BmN80%3D) |
341
- | RelayBox Arduino | For laser control using camera GPIO signals | [RelayBox](https://github.com/samhitech/RelayBox) |
342
- | miEye OSF Project Parts List | Parts list of miEye OSF Project | [Repo Link](https://osf.io/j2fqy/) |
336
+ | Hardware | Description | Driver | Link |
337
+ | --------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------------------- | :------: | :----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
338
+ | Integrated Optics MatchBox | Multi-wavelength Laser Combiner, Single Laser MatchBox | `Native` | [Products Link](https://integratedoptics.com/products/wavelength-combiners) |
339
+ | Piezo Concept FOC | Nanopositioner for microscope objectives | `Native` | [Piezo Concept FOC](https://piezoconcept-store.squarespace.com/1-axis/p/foc) |
340
+ | Thorlabs Elliptec ELL6/ELL9/ELL12 | Dual/Four/Six-Position Slider | `Native` | [ELL6](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL6), [ELL9](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL9), [ELL12](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL12) |
341
+ | Thorlabs Elliptec ELL14 | Rotation Mount: SM1 Threaded | `Native` | [ELL14](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL14) |
342
+ | Thorlabs Elliptec ELL20 | Linear Stage: 60 mm Travel | `Native` | [ELL20](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL20) |
343
+ | Thorlabs KDC101 | Kinesis Controller for Z825B/[Z925B](https://www.thorlabs.com/thorproduct.cfm?partnumber=Z925B) actuators (Activate USB VCP to access the COM port in device manager) | `Native` | [KDC101](https://www.thorlabs.com/thorproduct.cfm?partnumber=KDC101) |
344
+ | Parallax TSL1401-DB (#28317) | Linescan Camera Module | `Native` | [Parallax TSL1401-DB](https://eu.mouser.com/ProductDetail/Parallax/28317?qs=%2Fha2pyFaduiCRhuOAXMuCmQIeG1Q3R01m6Y1EH%252BmN80%3D) |
345
+ | RelayBox Arduino | For laser control using camera GPIO signals | `Native` | [RelayBox](https://github.com/samhitech/RelayBox) |
346
+ | miEye OSF Project Parts List | Parts list of miEye OSF Project | - | [Repo Link](https://osf.io/j2fqy/) |
343
347
 
344
348
  ## Pycro-Manager Integration
345
349
 
346
350
  Integration with Pycro-Manager is now implemented, providing support for managing headless instances and `Core` instances. This allows for seamless control and configuration of hardware devices through the Pycro-Manager framework.
347
351
 
352
+ ![pycromanager integration](https://github.com/user-attachments/assets/73893f9f-c1ef-438f-a884-ec3f60f00118)
353
+
348
354
  ### Features
349
355
 
350
356
  - **Headless Manager**: Manage multiple headless Micro-Manager instances (JavaBackend), including starting, stopping, and saving configurations.
351
- - **Core Instances Manager**: Handle multiple PycroCore instances for device communication and control.
357
+ - **Core Instances Manager**: Handle multiple `PycroCore` instances for device communication and control.
352
358
 
353
359
  ### Integrated Hardware
354
360
 
355
361
  The following table lists the hardware devices that are integrated and supported by the `miEye module`:
356
362
 
357
- | Device | Description |
358
- | :----: | ------------------------------------------------------------------------------------------- |
359
- | Camera | Access Micro-Manager cameras (mainly done for focus stabilization). |
360
- | ZStage | Access Micro-Manager Z-stage devices for focus stabilization and axial positioning control. |
363
+ | Device | Description |
364
+ | :------: | ------------------------------------------------------------------------------------------- |
365
+ | `Camera` | Access Micro-Manager cameras (mainly done for focus stabilization). |
366
+ | `ZStage` | Access Micro-Manager Z-stage devices for focus stabilization and axial positioning control. |
361
367
 
362
368
  ### How to Use
363
369
 
@@ -377,26 +383,30 @@ The following table lists the hardware devices that are integrated and supported
377
383
 
378
384
  _Accessing other types of devices is under development!_
379
385
 
380
- _Note: Currently, only acquisition has been tested with the developed `PycroPanel`. Full implementation of focus stabilization is planned for future updates by implementing further stage-related widgets._
386
+ > **Note:** Currently, only acquisition and focus stabilization features are implemented. Support for additional hardware and stage-related controls is under active development.
381
387
 
382
- ![pycromanager integration](https://github.com/user-attachments/assets/73893f9f-c1ef-438f-a884-ec3f60f00118)
388
+ ### Step-by-step Instructions
389
+
390
+ For comprehensive step-by-step instructions, please refer to the [Pycro-Manager Integration Guide](https://github.com/samhitech/microEye/tree/main/guide/pycromanager).
383
391
 
384
392
  ## Authors
385
393
 
386
- [Mohammad Nour Alsamsam](https://tutkuslab.github.io/team/MNA/), PhD student @ Vilnius University.
394
+ [`Mohammad Nour Alsamsam`](https://tutkuslab.github.io/team/MNA/)
387
395
 
388
396
  [![Twitter URL](https://img.shields.io/twitter/url/https/twitter.com/samhightech.svg?style=social&label=Follow%20%40samhightech)](https://twitter.com/samhightech)
389
397
 
390
398
  ## People Involved
391
399
 
392
- **PhD supervision:** [Dr. Marijonas Tutkus](https://tutkuslab.github.io/team/MT/)
400
+ **PhD supervision:**
401
+
402
+ [`Dr. Marijonas Tutkus`](https://tutkuslab.github.io/team/MT/)
393
403
 
394
404
  [![Twitter URL](https://img.shields.io/twitter/url/https/twitter.com/MTutkus.svg?style=social&label=Follow%20%40MTutkus)](https://twitter.com/MTutkus)
395
405
 
396
406
  **Sample preparation, experiments and testing:**
397
407
 
398
- - [Aurimas Kopūstas](https://tutkuslab.github.io/team/AK/)
399
- - [Tutkus Lab Team](https://tutkuslab.github.io/team/)
408
+ [`Aurimas Kopūstas`](https://tutkuslab.github.io/team/AK/)
409
+ [`Tutkus Lab Team`](https://tutkuslab.github.io/team/)
400
410
 
401
411
  ## Acknowledgement
402
412
 
@@ -408,7 +418,7 @@ Research and access to intruments and samples is credited to:
408
418
 
409
419
  Special thanks to the following projects and libraries that make this work possible:
410
420
 
411
- - **SMAP/fit3Dcspline**: The original code, provided as a part of the Ries group [SMAP software](https://github.com/jries/SMAP/tree/master/fit3Dcspline). The `pyfit3Dcspline` module in our project is a Python adaptation of this functionality, offering both CPU and GPU accelerated fitting of Single-Molecule Localization Microscopy (SMLM) data. For more details, refer to the pyfit3Dcspline [README](https://github.com/samhitech/microEye/tree/main/src/microEye/analysis/fitting/pyfit3Dcspline/README.md).
421
+ - **SMAP/fit3Dcspline**: The original code, provided as a part of the Ries group [`SMAP software`](https://github.com/jries/SMAP/tree/master/fit3Dcspline). The `pyfit3Dcspline` module in our project is a Python adaptation of this functionality, offering both CPU and GPU accelerated fitting of Single-Molecule Localization Microscopy (SMLM) data. For more details, refer to the pyfit3Dcspline [`README`](https://github.com/samhitech/microEye/tree/main/src/microEye/analysis/fitting/pyfit3Dcspline/README.md).
412
422
 
413
423
  - **ACCéNT**: a partial implementation of the photon free calibration within the acquisition pipeline which generates pixel-wise mean and variance images.
414
424
 
@@ -422,7 +432,7 @@ Special thanks to the following projects and libraries that make this work possi
422
432
 
423
433
  > Raw data to results: a hands-on introduction and overview of computational analysis for single-molecule localization microscopy", Martens et al., (2022), Frontiers in Bioinformatics. [Paper](https://www.frontiersin.org/articles/10.3389/fbinf.2021.817254)
424
434
 
425
- - **TARDIS (Temporal Analysis of Relative Distances)**: We have developed a partial Python implementation of TARDIS without fitting for now. For more information, refer to the [TARDIS software releases](https://github.com/kjamartens/TARDIS-public). The underlying algorithms and scientific details of TARDIS are detailed in the manuscript:
435
+ - **TARDIS (Temporal Analysis of Relative Distances)**: We have developed a partial Python implementation of TARDIS without fitting for now. For more information, refer to the [`TARDIS` software releases](https://github.com/kjamartens/TARDIS-public). The underlying algorithms and scientific details of TARDIS are detailed in the manuscript:
426
436
  > Martens et al., “Temporal analysis of relative distances (TARDIS) is a robust, parameter-free alternative to single-particle tracking”, Nature Methods (2024). [Article](https://rdcu.be/dv1sr)
427
437
 
428
438
  **Note**: I'm committed to maintaining an accurate acknowledgment list for our project. However, if I inadvertently miss acknowledging your work, please don't hesitate to reach out to us. I appreciate your understanding, and I'm doing my best to manage all acknowledgments amidst my other responsibilities.