microeye 2.3.13__tar.gz → 2.4.0a2__tar.gz
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- {microeye-2.3.13/src/microeye.egg-info → microeye-2.4.0a2}/PKG-INFO +90 -80
- {microeye-2.3.13 → microeye-2.4.0a2}/README.md +86 -76
- microeye-2.4.0a2/src/microEye/_version.py +2 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/filters/base.py +2 -1
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/multi_viewer.py +1 -1
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/__init__.py +1 -2
- microeye-2.4.0a2/src/microEye/hardware/cams/basler/basler_cam.py +1014 -0
- microeye-2.4.0a2/src/microEye/hardware/cams/basler/basler_panel.py +394 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/camera_list.py +172 -160
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/camera_options.py +17 -30
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/camera_panel.py +204 -60
- microeye-2.4.0a2/src/microEye/hardware/cams/dummy/dummy_panel.py +184 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/micam.py +480 -38
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/pco/pco_cam.py +95 -9
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/pco/pco_panel.py +5 -169
- microeye-2.4.0a2/src/microEye/hardware/cams/pycromanager/__init__.py +1 -0
- microeye-2.4.0a2/src/microEye/hardware/cams/pycromanager/pycro_cam.py +194 -0
- {microeye-2.3.13/src/microEye/hardware/pycromanager/widgets → microeye-2.4.0a2/src/microEye/hardware/cams/pycromanager}/pycro_panel.py +19 -104
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/thorlabs/thorlabs.py +141 -4
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/thorlabs/thorlabs_panel.py +26 -233
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/ueye/ueye_camera.py +148 -3
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/ueye/ueye_panel.py +23 -218
- microeye-2.4.0a2/src/microEye/hardware/cams/vimba/__init__.py +7 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/vimba/vimba_cam.py +398 -66
- microeye-2.4.0a2/src/microEye/hardware/cams/vimba/vimba_panel.py +393 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/mieye/acquisition_manager.py +42 -19
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/mieye/devices_manager.py +100 -226
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/mieye/miEye.py +66 -65
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/core.py +1 -0
- microeye-2.4.0a2/src/microEye/hardware/pycromanager/devices.py +214 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/widgets/__init__.py +0 -1
- microeye-2.4.0a2/src/microEye/hardware/stages/kinesis/__init__.py +1 -0
- microeye-2.4.0a2/src/microEye/hardware/stages/kinesis/kinesis.py +164 -0
- microeye-2.4.0a2/src/microEye/hardware/stages/manager.py +456 -0
- microeye-2.4.0a2/src/microEye/hardware/stages/piezon_concept/__init__.py +1 -0
- microeye-2.3.13/src/microEye/hardware/stages/piezo_concept.py → microeye-2.4.0a2/src/microEye/hardware/stages/piezon_concept/foc.py +22 -32
- microeye-2.4.0a2/src/microEye/hardware/stages/pycromanager/__init__.py +1 -0
- microeye-2.4.0a2/src/microEye/hardware/stages/pycromanager/core.py +232 -0
- microeye-2.4.0a2/src/microEye/hardware/stages/smaract/__init__.py +1 -0
- microeye-2.4.0a2/src/microEye/hardware/stages/smaract/mcs2.py +123 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/stabilizer.py +1 -1
- microeye-2.4.0a2/src/microEye/hardware/stages/stage.py +870 -0
- microeye-2.4.0a2/src/microEye/hardware/stages/view.py +409 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/widgets/controller.py +46 -80
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/widgets/devices.py +0 -29
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/widgets/scan_acquisition.py +9 -4
- microeye-2.4.0a2/src/microEye/hardware/widgets/slides.py +550 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/launcher.py +7 -9
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/micro_launcher.py +10 -6
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/utils/parameter_tree.py +2 -2
- {microeye-2.3.13 → microeye-2.4.0a2/src/microeye.egg-info}/PKG-INFO +90 -80
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microeye.egg-info/SOURCES.txt +13 -2
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microeye.egg-info/requires.txt +3 -3
- microeye-2.3.13/src/microEye/_version.py +0 -2
- microeye-2.3.13/src/microEye/hardware/cams/basler/basler_cam.py +0 -413
- microeye-2.3.13/src/microEye/hardware/cams/dummy/dummy_panel.py +0 -695
- microeye-2.3.13/src/microEye/hardware/cams/vimba/vimba_panel.py +0 -745
- microeye-2.3.13/src/microEye/hardware/lasers/__init__.py +0 -1
- microeye-2.3.13/src/microEye/hardware/pycromanager/devices.py +0 -487
- microeye-2.3.13/src/microEye/hardware/stages/kinesis/__init__.py +0 -1
- microeye-2.3.13/src/microEye/hardware/stages/kinesis/kinesis.py +0 -771
- microeye-2.3.13/src/microEye/hardware/stages/stage.py +0 -851
- {microeye-2.3.13 → microeye-2.4.0a2}/LICENSE +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/MANIFEST.in +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/pyproject.toml +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/setup.cfg +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/setup.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/checklist_dialog.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/cmosMaps.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/filters/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/filters/spatial.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/filters/temporal.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/fit.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/nena.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/phasor_fit.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/processing.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/extract.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/rubost_mean.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/spline.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/stats/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/stats/core.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/stats/curve_fit.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/stats/io.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/stats/slope_fit.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/temp.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/psf/test.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/CPU/CPUfunctions.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/CPU/CPUmleFit_LM.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/CPU/CPUsplineLib.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/CPU/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/GPU/GPUfunctions.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/GPU/GPUmleFit_LM_EMCCD.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/GPU/GPUmleFit_LM_sCMOS.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/GPU/GPUsplineLib.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/GPU/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/constants.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/pyfit3Dcspline/mainfunctions.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/rcc.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/results.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/results_stats.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/fitting/tardis.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/processing/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/processing/frc.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/rendering/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/rendering/base.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/rendering/cloud.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/rendering/core.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/rendering/volumetric.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/tools/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/tools/kymograms.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/tools/roi_selectors.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/utils/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/utils/coordinates.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/utils/images.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/utils/windows.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/image_options_widget.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/images.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/layers_widget.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/localizations.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/psf.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/analysis/viewer/volume.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/basler/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/camera_calibration.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/dummy/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/jobs.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/line_profiler.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/linescan/IR_Cam.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/linescan/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/pco/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/pco/enums.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/shortcuts.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/thorlabs/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/cams/ueye/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/device.py +0 -0
- {microeye-2.3.13/src/microEye/hardware/cams/vimba → microeye-2.4.0a2/src/microEye/hardware/lasers}/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/lasers/io_matchbox.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/lasers/io_params.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/lasers/io_single_laser.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/lasers/laser_relay.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/mieye/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/misc/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/misc/acquisition_view.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/misc/reglo.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/misc/temp.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/port_config.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/protocols/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/protocols/actions.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/protocols/actions_items.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/protocols/designer.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/protocols/scene_manager.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/protocols/serialization.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/enums.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/headless.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/utils.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/widgets/bridges.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/widgets/headless_manager.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/pycromanager/widgets/headless_options.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/baseDevice.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/device.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/deviceID.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/devicePort.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/deviceStatus.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/devicesView.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/ellDevices.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/messageUpdater.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/motorInfo.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/stage.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/elliptec/test.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/kinesis/kdc101/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/kinesis/kdc101/enums.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/kinesis/kdc101/factory.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/stages/kinesis/kdc101/kdc101.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/widgets/__init__.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/widgets/focusWidget.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/hardware/widgets/qlist_slider.py +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/1024.png +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/128.png +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/16.png +0 -0
- {microeye-2.3.13 → microeye-2.4.0a2}/src/microEye/icons/24.png +0 -0
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Summary: A python toolkit for fluorescence microscopy that features hardware control, data analysis and vizualization for super-resolution single-molecule localization microscopy and single-partical tracking.
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This toolkit is compatible with the [hardware](#hardware) used in our microscope. For further details, refer to the [miEye microscope paper](https://doi.org/10.1016/j.ohx.2022.e00368) and [OSF project](http://doi.org/10.17605/osf.io/j2fqy).
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```bash
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- [Features](#features)
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| Data Analysis and Visualization | GUI and UI Development | Code Quality and Formatting | Image and Video Processing | File and Data Storage | Other Utilities | Microscopy |
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| IDS uEye UI-3060CP Rev. 2 | IDS industrial-grade CMOS cameras | [Product Link](https://en.ids-imaging.com/store/products/cameras/ui-3060cp-rev-2.html) |
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| Allied Vision Alvium 1800 | Allied Vision industrial-grade CMOS cameras (U-158m, U-511m) | [Product Link](https://www.alliedvision.com/en/products/alvium-configurator/alvium-1800-u/158/#_configurator) |
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| IDS uEye UI-3060CP Rev. 2 | IDS industrial-grade CMOS cameras | `IDS uEye SDK 4.96.1` `pyueye` | [Product Link](https://en.ids-imaging.com/store/products/cameras/ui-3060cp-rev-2.html) |
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| Thorlabs DCC1545M | DCx camera using UC480 driver | `ThorCam 3.7.0` | [Product Link](https://www.thorlabs.com/thorProduct.cfm?partNumber=DCC1545M) |
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| Allied Vision Alvium 1800 | Allied Vision industrial-grade CMOS cameras (U-158m, U-511m) | `Vimba X SDK` `vmbpy` | [Product Link](https://www.alliedvision.com/en/products/alvium-configurator/alvium-1800-u/158/#_configurator) |
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| Excelitas PCO sCMOS | Excelitas pco.edge 4.2 LT USB sCMOS Camera | `pco.sdk` `pco.python` | [Product Link](https://www.excelitas.com/product/pcoedge-42-lt-usb-scmos-camera) |
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| Pycro-camera | Access to cameras via Micro-Manager drivers/adapters using Pycro-Manager | `uManager` `Pycro-Manager` | - |
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### Additional Hardware
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| Hardware | Description |
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| --------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
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| Integrated Optics MatchBox | Multi-wavelength Laser Combiner, Single Laser MatchBox | [Products Link](https://integratedoptics.com/products/wavelength-combiners)
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| Piezo Concept FOC | Nanopositioner for microscope objectives | [Piezo Concept FOC](https://piezoconcept-store.squarespace.com/1-axis/p/foc)
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| Thorlabs Elliptec ELL6/ELL9/ELL12 | Dual/Four/Six-Position Slider | [ELL6](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL6), [ELL9](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL9), [ELL12](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL12) |
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| Thorlabs Elliptec ELL14 | Rotation Mount: SM1 Threaded | [ELL14](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL14)
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| Thorlabs Elliptec ELL20 | Linear Stage: 60 mm Travel | [ELL20](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL20)
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| Thorlabs KDC101 | Kinesis Controller for Z825B/[Z925B](https://www.thorlabs.com/thorproduct.cfm?partnumber=Z925B) actuators (Activate USB VCP to access the COM port in device manager) | [KDC101](https://www.thorlabs.com/thorproduct.cfm?partnumber=KDC101)
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| Parallax TSL1401-DB (#28317) | Linescan Camera Module | [Parallax TSL1401-DB](https://eu.mouser.com/ProductDetail/Parallax/28317?qs=%2Fha2pyFaduiCRhuOAXMuCmQIeG1Q3R01m6Y1EH%252BmN80%3D)
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| RelayBox Arduino | For laser control using camera GPIO signals | [RelayBox](https://github.com/samhitech/RelayBox)
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| miEye OSF Project Parts List | Parts list of miEye OSF Project |
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| Hardware | Description | Driver | Link |
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| --------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------------------- | :------: | :----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
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| Integrated Optics MatchBox | Multi-wavelength Laser Combiner, Single Laser MatchBox | `Native` | [Products Link](https://integratedoptics.com/products/wavelength-combiners) |
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| Piezo Concept FOC | Nanopositioner for microscope objectives | `Native` | [Piezo Concept FOC](https://piezoconcept-store.squarespace.com/1-axis/p/foc) |
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| Thorlabs Elliptec ELL6/ELL9/ELL12 | Dual/Four/Six-Position Slider | `Native` | [ELL6](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL6), [ELL9](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL9), [ELL12](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL12) |
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| Thorlabs Elliptec ELL14 | Rotation Mount: SM1 Threaded | `Native` | [ELL14](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL14) |
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| Thorlabs Elliptec ELL20 | Linear Stage: 60 mm Travel | `Native` | [ELL20](https://www.thorlabs.com/thorproduct.cfm?partnumber=ELL20) |
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| Thorlabs KDC101 | Kinesis Controller for Z825B/[Z925B](https://www.thorlabs.com/thorproduct.cfm?partnumber=Z925B) actuators (Activate USB VCP to access the COM port in device manager) | `Native` | [KDC101](https://www.thorlabs.com/thorproduct.cfm?partnumber=KDC101) |
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| Parallax TSL1401-DB (#28317) | Linescan Camera Module | `Native` | [Parallax TSL1401-DB](https://eu.mouser.com/ProductDetail/Parallax/28317?qs=%2Fha2pyFaduiCRhuOAXMuCmQIeG1Q3R01m6Y1EH%252BmN80%3D) |
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| RelayBox Arduino | For laser control using camera GPIO signals | `Native` | [RelayBox](https://github.com/samhitech/RelayBox) |
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| miEye OSF Project Parts List | Parts list of miEye OSF Project | - | [Repo Link](https://osf.io/j2fqy/) |
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## Pycro-Manager Integration
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Integration with Pycro-Manager is now implemented, providing support for managing headless instances and `Core` instances. This allows for seamless control and configuration of hardware devices through the Pycro-Manager framework.
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### Features
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- **Headless Manager**: Manage multiple headless Micro-Manager instances (JavaBackend), including starting, stopping, and saving configurations.
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- **Core Instances Manager**: Handle multiple PycroCore instances for device communication and control.
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- **Core Instances Manager**: Handle multiple `PycroCore` instances for device communication and control.
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### Integrated Hardware
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The following table lists the hardware devices that are integrated and supported by the `miEye module`:
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| Camera | Access Micro-Manager cameras (mainly done for focus stabilization). |
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| ZStage | Access Micro-Manager Z-stage devices for focus stabilization and axial positioning control. |
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| Device | Description |
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| :------: | ------------------------------------------------------------------------------------------- |
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| `Camera` | Access Micro-Manager cameras (mainly done for focus stabilization). |
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| `ZStage` | Access Micro-Manager Z-stage devices for focus stabilization and axial positioning control. |
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### How to Use
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_Accessing other types of devices is under development!_
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> **Note:** Currently, only acquisition and focus stabilization features are implemented. Support for additional hardware and stage-related controls is under active development.
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|
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### Step-by-step Instructions
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+
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+
For comprehensive step-by-step instructions, please refer to the [Pycro-Manager Integration Guide](https://github.com/samhitech/microEye/tree/main/guide/pycromanager).
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## Authors
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[Mohammad Nour Alsamsam](https://tutkuslab.github.io/team/MNA/)
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[`Mohammad Nour Alsamsam`](https://tutkuslab.github.io/team/MNA/)
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[](https://twitter.com/samhightech)
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## People Involved
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**PhD supervision:**
|
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+
**PhD supervision:**
|
|
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+
|
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[`Dr. Marijonas Tutkus`](https://tutkuslab.github.io/team/MT/)
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[](https://twitter.com/MTutkus)
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**Sample preparation, experiments and testing:**
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|
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-
|
|
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-
|
|
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+
[`Aurimas Kopūstas`](https://tutkuslab.github.io/team/AK/)
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+
[`Tutkus Lab Team`](https://tutkuslab.github.io/team/)
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## Acknowledgement
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|
|
@@ -408,7 +418,7 @@ Research and access to intruments and samples is credited to:
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Special thanks to the following projects and libraries that make this work possible:
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- **SMAP/fit3Dcspline**: The original code, provided as a part of the Ries group [SMAP software](https://github.com/jries/SMAP/tree/master/fit3Dcspline). The `pyfit3Dcspline` module in our project is a Python adaptation of this functionality, offering both CPU and GPU accelerated fitting of Single-Molecule Localization Microscopy (SMLM) data. For more details, refer to the pyfit3Dcspline [README](https://github.com/samhitech/microEye/tree/main/src/microEye/analysis/fitting/pyfit3Dcspline/README.md).
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- **SMAP/fit3Dcspline**: The original code, provided as a part of the Ries group [`SMAP software`](https://github.com/jries/SMAP/tree/master/fit3Dcspline). The `pyfit3Dcspline` module in our project is a Python adaptation of this functionality, offering both CPU and GPU accelerated fitting of Single-Molecule Localization Microscopy (SMLM) data. For more details, refer to the pyfit3Dcspline [`README`](https://github.com/samhitech/microEye/tree/main/src/microEye/analysis/fitting/pyfit3Dcspline/README.md).
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- **ACCéNT**: a partial implementation of the photon free calibration within the acquisition pipeline which generates pixel-wise mean and variance images.
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@@ -422,7 +432,7 @@ Special thanks to the following projects and libraries that make this work possi
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> Raw data to results: a hands-on introduction and overview of computational analysis for single-molecule localization microscopy", Martens et al., (2022), Frontiers in Bioinformatics. [Paper](https://www.frontiersin.org/articles/10.3389/fbinf.2021.817254)
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- **TARDIS (Temporal Analysis of Relative Distances)**: We have developed a partial Python implementation of TARDIS without fitting for now. For more information, refer to the [TARDIS software releases](https://github.com/kjamartens/TARDIS-public). The underlying algorithms and scientific details of TARDIS are detailed in the manuscript:
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+
- **TARDIS (Temporal Analysis of Relative Distances)**: We have developed a partial Python implementation of TARDIS without fitting for now. For more information, refer to the [`TARDIS` software releases](https://github.com/kjamartens/TARDIS-public). The underlying algorithms and scientific details of TARDIS are detailed in the manuscript:
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> Martens et al., “Temporal analysis of relative distances (TARDIS) is a robust, parameter-free alternative to single-particle tracking”, Nature Methods (2024). [Article](https://rdcu.be/dv1sr)
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**Note**: I'm committed to maintaining an accurate acknowledgment list for our project. However, if I inadvertently miss acknowledging your work, please don't hesitate to reach out to us. I appreciate your understanding, and I'm doing my best to manage all acknowledgments amidst my other responsibilities.
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