geoseeq 0.5.6a10__tar.gz → 0.5.6a11__tar.gz

{geoseeq-0.5.6a10 → geoseeq-0.5.6a11}/PKG-INFO

@@ -1,6 +1,6 @@
 Metadata-Version: 2.1
 Name: geoseeq
-Version: 0.5.6a10
+Version: 0.5.6a11
 Summary: GeoSeeq command line tools and python API
 Author: David C. Danko
 Author-email: "David C. Danko" <dcdanko@biotia.io>

{geoseeq-0.5.6a10 → geoseeq-0.5.6a11}/geoseeq/cli/main.py

@@ -53,7 +53,7 @@ def version():
     Use of this tool implies acceptance of the GeoSeeq End User License Agreement.
     Run `geoseeq eula show` to view the EULA.
     """
-    click.echo('0.5.6a10')  # remember to update setup
+    click.echo('0.5.6a11')  # remember to update setup
 
 
 @main.group('advanced')

{geoseeq-0.5.6a10 → geoseeq-0.5.6a11}/geoseeq/cli/upload/upload.py

@@ -37,6 +37,7 @@ hidden_option = click.option('--hidden/--no-hidden', default=False, help='Upload
 @click.command('files')
 @use_common_state
 @click.option('--cores', default=1, help='Number of uploads to run in parallel')
+@click.option('--threads-per-upload', default=4, help='Number of threads used to upload each file')
 @yes_option
 @private_option
 @link_option
@@ -47,7 +48,7 @@ hidden_option = click.option('--hidden/--no-hidden', default=False, help='Upload
               help='Specify a different name for the file on GeoSeeq than the local file name.')
 @folder_id_arg
 @click.argument('file_paths', type=click.Path(exists=True), nargs=-1)
-def cli_upload_file(state, cores, yes, private, link_type, recursive, hidden, no_new_versions, geoseeq_file_name, folder_id, file_paths):
+def cli_upload_file(state, cores, threads_per_upload, yes, private, link_type, recursive, hidden, no_new_versions, geoseeq_file_name, folder_id, file_paths):
     """Upload files to GeoSeeq.
 
     This command uploads files to either a sample or project on GeoSeeq. It can be used to upload
@@ -106,6 +107,7 @@ def cli_upload_file(state, cores, yes, private, link_type, recursive, hidden, no
     
     upload_manager = GeoSeeqUploadManager(
         n_parallel_uploads=cores,
+        threads_per_upload=threads_per_upload,
         link_type=link_type,
         progress_tracker_factory=PBarManager().get_new_bar,
         log_level=state.log_level,

{geoseeq-0.5.6a10 → geoseeq-0.5.6a11}/geoseeq/cli/upload/upload_reads.py

@@ -132,6 +132,28 @@ def flatten_list_of_fastqs(filepaths):
     return flattened
 
 
+def _is_bam(path):
+    for ext in ['.bam', '.bai']:
+        if path.endswith(ext):
+            return True
+    return False
+
+
+def flatten_list_of_bams(filepaths):
+    """Turn a list of bam filepaths and txt files containing bam filepaths into a single list of bam filepaths."""
+    flattened = []
+    for path in filepaths:
+        if _is_bam(path):
+            flattened.append(path)
+        else:
+            with open(path) as f:
+                for line in f:
+                    line = line.strip()
+                    if line and not line.startswith('#'):
+                        flattened.append(line)
+    return flattened
+
+
 
 @click.command('reads')
 @use_common_state
@@ -200,3 +222,67 @@ def cli_upload_reads_wizard(state, cores, overwrite, yes, regex, private, link_t
     regex = _get_regex(knex, filepaths, module_name, proj, regex)
     groups = _group_files(knex, filepaths, module_name, regex, yes)
     _do_upload(groups, module_name, link_type, proj, filepaths, overwrite, no_new_versions, cores, state)
+
+
+# @click.command('bam')
+# @use_common_state
+# @click.option('--genome', default=None, help='The genome aligned to the BAM files. Should be in 2bit format.')
+# @click.option('--cores', default=1, help='Number of uploads to run in parallel')
+# @overwrite_option
+# @yes_option
+# @click.option('--regex', default=None, help='An optional regex to use to extract sample names from the file names')
+# @private_option
+# @link_option
+# @no_new_versions_option
+# @project_id_arg
+# @click.argument('files', type=click.Path(exists=True), nargs=-1)
+# def cli_upload_bams(state, genome, cores, overwrite, yes, regex, private, link_type, no_new_versions, project_id, files):
+    """Upload BAM files to GeoSeeq.
+
+    This command automatically groups bams with their index files.
+
+    ---
+
+    Example Usage:
+
+    \b
+    # Upload a list of BAM files to a project, useful if you have hundreds of files
+    $ ls -1 path/to/bam/files/*.bam > file_list.txt
+    $ geoseeq upload bams "GeoSeeq/Example CLI Project" file_list.txt
+
+    \b
+    # Upload all the BAM files in a directory to a project with BAM indexes
+    $ geoseeq upload bams ed59b913-91ec-489b-a1b9-4ea137a6e5cf path/to/bam/files/*.bam path/to/bam/files/*.bam.bai
+
+    \b
+    # Upload all the BAM files in a directory to a project, performing 4 uploads in parallel
+    $ geoseeq upload bams --cores 4 ed59b913-91ec-489b-a1b9-4ea137a6e5cf path/to/bam/files/*.bam
+
+    \b
+    # Upload a list of BAM files to a project, automatically creating a new project and overwriting existing files
+    $ ls -1 path/to/bam/files/*.bam > file_list.txt
+    $ geoseeq upload bams --yes --overwrite "GeoSeeq/Example CLI Project" file_list.txt
+
+    ---
+
+    Command Arguments:
+    
+    [PROJECT_ID] Can be a project UUID, GeoSeeq Resource Number (GRN), or an
+    organization name and project name separated by a slash.
+
+    \b
+    Examples: 
+     - Name pair: "GeoSeeq/Example CLI Project"
+     - UUID: "ed59b913-91ec-489b-a1b9-4ea137a6e5cf"
+     - GRN: "grn:gs1:project:ed59b913-91ec-489b-a1b9-4ea137a6e5cf"
+
+    \b
+    [FILES...] can be paths to BAM files or a file containing a list of paths, or a mix of both.
+    Example: "path/to/bam/files
+    """
+    knex = state.get_knex()
+    proj = handle_project_id(knex, project_id, yes, private)
+    filepaths = {basename(line): line for line in flatten_list_of_bams(files)}
+    click.echo(f'Found {len(filepaths)} files to upload.', err=True)
+    groups = _group_files(knex, filepaths, 'bam::bam', regex, yes)
+    _do_upload(groups, 'bam::bam', link_type, proj, filepaths, overwrite, no_new_versions, cores, state)

{geoseeq-0.5.6a10 → geoseeq-0.5.6a11}/geoseeq/result/file_download.py

@@ -46,6 +46,11 @@ def guess_download_kind(url):
 
 def download_url(url, kind='guess', filename=None, head=None, progress_tracker=None):
     """Return a local filepath to the downloaded file. Download the file."""
+    if filename and isfile(filename):
+        file_size = getsize(filename)
+        if file_size > 0:
+            logger.info(f"File already exists: {filename}. Not overwriting.")
+            return filename
     if kind == 'guess':
         kind = guess_download_kind(url)
         logger.info(f"Guessed download kind: {kind} for {url}")

{geoseeq-0.5.6a10 → geoseeq-0.5.6a11}/geoseeq/result/file_upload.py

@@ -31,14 +31,30 @@ class FileChunker:
                     chunk = f.read(self.chunk_size)
                     self.loaded_parts.append(chunk)
         return self  # convenience for chaining
+    
+    def chunk_is_preloaded(self, num):
+        return len(self.loaded_parts) > num and self.loaded_parts[num]
+    
+    def read_one_chunk(self, num):
+        if not self.chunk_is_preloaded(num):
+            logger.debug(f"Reading chunk {num} from {self.filepath}")
+            with open(self.filepath, "rb") as f:
+                f.seek(num * self.chunk_size)
+                chunk = f.read(self.chunk_size)
+                return chunk
+        return self.loaded_parts[num]
 
     def get_chunk(self, num):
-        self.load_all_chunks()
-        return self.loaded_parts[num]
+        if self.chunk_is_preloaded(num):
+            return self.loaded_parts[num]
+        return self.read_one_chunk(num)
     
     def get_chunk_size(self, num):
-        self.load_all_chunks()
-        return len(self.loaded_parts[num])
+        if num < (self.n_parts - 1):  # all but the last chunk
+            return self.chunk_size
+        if self.chunk_is_preloaded(num):  # last chunk, pre-loaded
+            return len(self.loaded_parts[num])
+        return len(self.read_one_chunk(num))  # last chunk, not pre-loaded
     
 
 class ResumableUploadTracker:
@@ -159,6 +175,7 @@ class ResultFileUpload:
         attempts = 0
         while attempts < max_retries:
             try:
+                logger.debug(f"Uploading part {num + 1} to {url}. Size: {len(file_chunk)} bytes.")
                 if session:
                     http_response = session.put(url, data=file_chunk)
                 else:
@@ -251,7 +268,12 @@ class ResultFileUpload:
                 resumable_upload_tracker.start_upload(upload_id, urls)
         logger.info(f'Starting upload for "{filepath}"')
         complete_parts = []
-        file_chunker = FileChunker(filepath, chunk_size).load_all_chunks()
+        file_chunker = FileChunker(filepath, chunk_size)
+        if file_chunker.file_size < 10 * FIVE_MB:
+            file_chunker.load_all_chunks()
+            logger.debug(f"Preloaded all chunks for {filepath}")
+        else:
+            logger.debug(f"Did not preload chunks for {filepath}")
         if progress_tracker: progress_tracker.set_num_chunks(file_chunker.file_size)
         complete_parts = self._upload_parts(
             file_chunker,

{geoseeq-0.5.6a10 → geoseeq-0.5.6a11}/geoseeq/upload_download_manager.py

@@ -21,7 +21,7 @@ def _make_in_process_logger(log_level):
 def _upload_one_file(args):
     (result_file, filepath, session, progress_tracker,
      link_type, overwrite, log_level, parallel_uploads,
-     use_cache, no_new_versions) = args
+     use_cache, no_new_versions, threads_per_upload) = args
     if parallel_uploads:
         _make_in_process_logger(log_level)
     if link_type == 'upload':
@@ -29,7 +29,8 @@ def _upload_one_file(args):
         result_file.upload_file(
             filepath,
             session=session, overwrite=overwrite, progress_tracker=progress_tracker,
-            threads=4, use_cache=use_cache, no_new_versions=no_new_versions
+            threads=threads_per_upload, use_cache=use_cache,
+            no_new_versions=no_new_versions
         )
     else:
         result_file.link_file(link_type, filepath)
@@ -40,6 +41,7 @@ class GeoSeeqUploadManager:
 
     def __init__(self,
                  n_parallel_uploads=1,
+                 threads_per_upload=4,
                  session=None,
                  link_type='upload',
                  progress_tracker_factory=None,
@@ -56,6 +58,7 @@ class GeoSeeqUploadManager:
         self._result_files = []
         self.no_new_versions = no_new_versions
         self.use_cache = use_cache
+        self.threads_per_upload = threads_per_upload
 
     def add_result_file(self, result_file, local_path):
         self._result_files.append((result_file, local_path))
@@ -80,7 +83,8 @@ class GeoSeeqUploadManager:
                 result_file, local_path,
                 self.session, self.progress_tracker_factory(local_path),
                 self.link_type, self.overwrite, self.log_level,
-                self.n_parallel_uploads > 1, self.use_cache, self.no_new_versions
+                self.n_parallel_uploads > 1, self.use_cache, self.no_new_versions,
+                self.threads_per_upload
             ) for result_file, local_path in self._result_files
         ]
         out = []

{geoseeq-0.5.6a10 → geoseeq-0.5.6a11}/geoseeq.egg-info/PKG-INFO

@@ -1,6 +1,6 @@
 Metadata-Version: 2.1
 Name: geoseeq
-Version: 0.5.6a10
+Version: 0.5.6a11
 Summary: GeoSeeq command line tools and python API
 Author: David C. Danko
 Author-email: "David C. Danko" <dcdanko@biotia.io>

{geoseeq-0.5.6a10 → geoseeq-0.5.6a11}/pyproject.toml

@@ -4,7 +4,7 @@ build-backend = "setuptools.build_meta"
 
 [project]
 name = "geoseeq"
-version = "0.5.6a10"
+version = "0.5.6a11"
 authors = [
   { name="David C. Danko", email="dcdanko@biotia.io" },
 ]