discotoolkit 1.1.3__tar.gz → 1.2.0__tar.gz
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- discotoolkit-1.2.0/MANIFEST.in +2 -0
- discotoolkit-1.2.0/PKG-INFO +140 -0
- discotoolkit-1.2.0/README.md +110 -0
- {discotoolkit-1.1.3 → discotoolkit-1.2.0}/discotoolkit/CELLiD.py +270 -98
- {discotoolkit-1.1.3 → discotoolkit-1.2.0}/discotoolkit/DiscoClass.py +7 -7
- discotoolkit-1.2.0/discotoolkit/DownloadDiscoData.py +238 -0
- {discotoolkit-1.1.3 → discotoolkit-1.2.0}/discotoolkit/GeneSearch.py +4 -2
- {discotoolkit-1.1.3 → discotoolkit-1.2.0}/discotoolkit/GetMetadata.py +157 -76
- discotoolkit-1.2.0/discotoolkit/GlobalVariable.py +81 -0
- discotoolkit-1.2.0/discotoolkit/Utilities.py +74 -0
- {discotoolkit-1.1.3 → discotoolkit-1.2.0}/discotoolkit/__init__.py +2 -1
- discotoolkit-1.2.0/discotoolkit.egg-info/PKG-INFO +140 -0
- {discotoolkit-1.1.3 → discotoolkit-1.2.0}/discotoolkit.egg-info/SOURCES.txt +7 -1
- {discotoolkit-1.1.3 → discotoolkit-1.2.0}/discotoolkit.egg-info/requires.txt +4 -0
- discotoolkit-1.2.0/pyproject.toml +3 -0
- discotoolkit-1.2.0/requirements.txt +12 -0
- {discotoolkit-1.1.3 → discotoolkit-1.2.0}/setup.py +5 -6
- discotoolkit-1.2.0/tests/test_server_contract.py +160 -0
- discotoolkit-1.2.0/tests/test_settings.py +90 -0
- discotoolkit-1.2.0/tests/test_version.py +41 -0
- discotoolkit-1.1.3/MANIFEST.in +0 -1
- discotoolkit-1.1.3/PKG-INFO +0 -13
- discotoolkit-1.1.3/README.md +0 -72
- discotoolkit-1.1.3/discotoolkit/DownloadDiscoData.py +0 -144
- discotoolkit-1.1.3/discotoolkit/GlobalVariable.py +0 -25
- discotoolkit-1.1.3/discotoolkit.egg-info/PKG-INFO +0 -13
- {discotoolkit-1.1.3 → discotoolkit-1.2.0}/discotoolkit/CellMapper.py +0 -0
- {discotoolkit-1.1.3 → discotoolkit-1.2.0}/discotoolkit.egg-info/dependency_links.txt +0 -0
- {discotoolkit-1.1.3 → discotoolkit-1.2.0}/discotoolkit.egg-info/top_level.txt +0 -0
- {discotoolkit-1.1.3 → discotoolkit-1.2.0}/setup.cfg +0 -0
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Metadata-Version: 2.4
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Name: discotoolkit
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Version: 1.2.0
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Summary: DISCOtoolkit is an python package that allows users to access data and use the tools provided by the DISCO database.
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Home-page: https://disco.bii.a-star.edu.sg/
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Author: Li Mengwei, Rom Uddamvathanak
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Author-email: uddamvathanak_rom@immunol.a-star.edu.sg
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Requires-Python: >=3.9
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Description-Content-Type: text/markdown
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Requires-Dist: pandas>=1.4.2
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Requires-Dist: numpy>=1.21.6
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Requires-Dist: scanpy>=1.9.3
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Requires-Dist: scipy>=1.10.1
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Requires-Dist: joblib>=1.1.0
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Requires-Dist: pandarallel>=1.6.5
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Requires-Dist: requests>=2.28.1
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Requires-Dist: colorcet>=3.0.1
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Requires-Dist: leidenalg
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Requires-Dist: h5py>=3.8
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Requires-Dist: matplotlib>=3.5
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Requires-Dist: seaborn>=0.12
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Dynamic: author
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Dynamic: author-email
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Dynamic: description
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Dynamic: description-content-type
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Dynamic: home-page
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Dynamic: requires-dist
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Dynamic: requires-python
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Dynamic: summary
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<!--
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* @Descripttion:
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* @version:
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* @Author: Mengwei Li
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* @Date: 2023-04-16 21:20:42
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* @LastEditors: Mengwei Li
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* @LastEditTime: 2023-04-16 21:22:03
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-->
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[](https://discotoolkit-py.readthedocs.io/en/latest/?badge=latest) [](https://pepy.tech/project/discotoolkit) [](https://pypi.org/project/discotoolkit)
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# DISCOtoolkit 1.2.0
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DISCOtoolkit is a Python package for accessing the data and tools of the [DISCO database](https://disco.bii.a-star.edu.sg/) (DISCO v1). Read the documentation at [discotoolkit-py.readthedocs.io](https://discotoolkit-py.readthedocs.io/en/latest/).
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- Filter and download DISCO data based on sample metadata and cell type information
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- Gene search: a gene's expression across cell types and tissues
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- CELLiD: cell type annotation
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- scEnrichment: gene set enrichment using DISCO DEGs
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> **DISCO v1 and v2.** The toolkit talks to **DISCO v1** by default. DISCO v2 has its own R package ([DISCOtoolkit](https://github.com/JinmiaoChenLab/DISCOtoolkit)); the Python package can still be pointed at a v2 server, see [Choosing a server](#choosing-a-server).
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## Quickstart (Google Colab)
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[](https://colab.research.google.com/github/JinmiaoChenLab/DISCOtoolkit_py/blob/main/docs/quickstart_disco_v1.ipynb)
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Installs, checks the server, filters, downloads and plots in about ten cells, with nothing to set up locally.
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## Installation
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Python 3.9 or newer. In your current environment:
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```
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pip install discotoolkit
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```
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To try the latest development version straight from GitHub:
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```
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pip install "git+https://github.com/JinmiaoChenLab/DISCOtoolkit_py.git"
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```
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To work on the code, or to test changes before they are released, install from a local clone. `-e` links the install to the folder, so edits take effect without reinstalling:
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```
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git clone https://github.com/JinmiaoChenLab/DISCOtoolkit_py.git
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cd DISCOtoolkit_py
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pip install -e .
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```
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`pip install discotoolkit` and a local install give the same code when the versions match. The difference is the source: PyPI delivers a released, packaged copy, while a local install uses the files in your folder.
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Dependencies (installed automatically): numpy, pandas, scanpy, scipy, joblib, pandarallel, requests, colorcet, leidenalg, h5py, matplotlib, seaborn.
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We recommend a virtual environment, for example with miniconda:
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```
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conda create --name disco python=3.10
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conda activate disco
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conda install ipykernel
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python -m ipykernel install --user --name disco --display-name "disco"
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python -m pip install -U discotoolkit
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```
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## Choosing a server
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```python
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import discotoolkit as dt
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dt.get_server() # 'https://disco.bii.a-star.edu.sg/disco_v3_api/' (DISCO v1, the default)
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dt.set_server("v2") # DISCO v2
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dt.set_server("https://my.server/disco_v3_api/") # any server with the same API
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```
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or set the `DISCO_API_URL` environment variable before importing the package.
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## Testing
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Two small test files need nothing beyond `requests`, so they run anywhere, including Colab:
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```
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python tests/test_settings.py # offline: server selection logic
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python tests/test_version.py # offline: setup.py and __init__.py agree on the version
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python tests/test_server_contract.py # online: does the server answer everything the toolkit needs?
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python tests/test_server_contract.py https://immunesinglecell.org/disco_v3_api/ # check another server
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```
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GitHub Actions runs these, and installs the package on Python 3.9-3.12, on every push. Maintainers: see [RELEASING.md](RELEASING.md) for how a release is published.
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`test_server_contract.py` checks status codes and the shape of each response (the columns and file types the toolkit reads). It only samples the large reference files, it does not download them.
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## Basic Usage
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Example in Jupyter notebook.
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<em>please select disco as the kernel for running the jupyter notebook</em>
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### [Quickstart for DISCO v1](https://github.com/JinmiaoChenLab/DISCOtoolkit_py/blob/main/docs/quickstart_disco_v1.ipynb)
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### [Filter and download DISCO data](https://github.com/JinmiaoChenLab/DISCOtoolkit_py/blob/main/docs/download_data.ipynb)
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### [Cell Type Annotation using CELLiD](https://github.com/JinmiaoChenLab/DISCOtoolkit_py/blob/main/docs/CELLiD_celltype_annotation.ipynb)
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### [scEnrichment](https://github.com/JinmiaoChenLab/DISCOtoolkit_py/blob/main/docs/scEnrichment.ipynb)
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## Citation
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1. [Li, Mengwei, et al. "DISCO: a database of Deeply Integrated human Single-Cell Omics data." Nucleic acids research 50.D1 (2022): D596-D602.](https://academic.oup.com/nar/article/50/D1/D596/6430491)
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2. [Mengwei Li, Kok Siong Ang, Brian Teo, Uddamvathanak Rom, Minh N Nguyen, Sebastian Maurer-Stroh, Jinmiao Chen. "Rediscovering publicly available single-cell data with the DISCO platform." Nucleic Acids Research (2024): gkae1108](https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae1108/7899529)
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## Follow us on our social media!
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- [HSCRM2](https://twitter.com/HSCRM2)
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- [JinmiaoChenLab Github repo](https://github.com/JinmiaoChenLab)
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<!--
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* @Descripttion:
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* @version:
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* @Author: Mengwei Li
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* @Date: 2023-04-16 21:20:42
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* @LastEditors: Mengwei Li
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* @LastEditTime: 2023-04-16 21:22:03
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-->
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[](https://discotoolkit-py.readthedocs.io/en/latest/?badge=latest) [](https://pepy.tech/project/discotoolkit) [](https://pypi.org/project/discotoolkit)
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# DISCOtoolkit 1.2.0
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DISCOtoolkit is a Python package for accessing the data and tools of the [DISCO database](https://disco.bii.a-star.edu.sg/) (DISCO v1). Read the documentation at [discotoolkit-py.readthedocs.io](https://discotoolkit-py.readthedocs.io/en/latest/).
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- Filter and download DISCO data based on sample metadata and cell type information
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- Gene search: a gene's expression across cell types and tissues
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- CELLiD: cell type annotation
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- scEnrichment: gene set enrichment using DISCO DEGs
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> **DISCO v1 and v2.** The toolkit talks to **DISCO v1** by default. DISCO v2 has its own R package ([DISCOtoolkit](https://github.com/JinmiaoChenLab/DISCOtoolkit)); the Python package can still be pointed at a v2 server, see [Choosing a server](#choosing-a-server).
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## Quickstart (Google Colab)
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[](https://colab.research.google.com/github/JinmiaoChenLab/DISCOtoolkit_py/blob/main/docs/quickstart_disco_v1.ipynb)
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Installs, checks the server, filters, downloads and plots in about ten cells, with nothing to set up locally.
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## Installation
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Python 3.9 or newer. In your current environment:
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```
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pip install discotoolkit
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```
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To try the latest development version straight from GitHub:
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```
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pip install "git+https://github.com/JinmiaoChenLab/DISCOtoolkit_py.git"
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```
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To work on the code, or to test changes before they are released, install from a local clone. `-e` links the install to the folder, so edits take effect without reinstalling:
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```
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git clone https://github.com/JinmiaoChenLab/DISCOtoolkit_py.git
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cd DISCOtoolkit_py
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pip install -e .
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```
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`pip install discotoolkit` and a local install give the same code when the versions match. The difference is the source: PyPI delivers a released, packaged copy, while a local install uses the files in your folder.
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Dependencies (installed automatically): numpy, pandas, scanpy, scipy, joblib, pandarallel, requests, colorcet, leidenalg, h5py, matplotlib, seaborn.
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We recommend a virtual environment, for example with miniconda:
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```
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conda create --name disco python=3.10
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conda activate disco
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conda install ipykernel
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python -m ipykernel install --user --name disco --display-name "disco"
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python -m pip install -U discotoolkit
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```
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## Choosing a server
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```python
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import discotoolkit as dt
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dt.get_server() # 'https://disco.bii.a-star.edu.sg/disco_v3_api/' (DISCO v1, the default)
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dt.set_server("v2") # DISCO v2
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dt.set_server("https://my.server/disco_v3_api/") # any server with the same API
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```
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or set the `DISCO_API_URL` environment variable before importing the package.
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## Testing
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Two small test files need nothing beyond `requests`, so they run anywhere, including Colab:
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```
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python tests/test_settings.py # offline: server selection logic
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python tests/test_version.py # offline: setup.py and __init__.py agree on the version
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python tests/test_server_contract.py # online: does the server answer everything the toolkit needs?
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python tests/test_server_contract.py https://immunesinglecell.org/disco_v3_api/ # check another server
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```
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GitHub Actions runs these, and installs the package on Python 3.9-3.12, on every push. Maintainers: see [RELEASING.md](RELEASING.md) for how a release is published.
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`test_server_contract.py` checks status codes and the shape of each response (the columns and file types the toolkit reads). It only samples the large reference files, it does not download them.
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## Basic Usage
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Example in Jupyter notebook.
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<em>please select disco as the kernel for running the jupyter notebook</em>
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### [Quickstart for DISCO v1](https://github.com/JinmiaoChenLab/DISCOtoolkit_py/blob/main/docs/quickstart_disco_v1.ipynb)
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### [Filter and download DISCO data](https://github.com/JinmiaoChenLab/DISCOtoolkit_py/blob/main/docs/download_data.ipynb)
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### [Cell Type Annotation using CELLiD](https://github.com/JinmiaoChenLab/DISCOtoolkit_py/blob/main/docs/CELLiD_celltype_annotation.ipynb)
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### [scEnrichment](https://github.com/JinmiaoChenLab/DISCOtoolkit_py/blob/main/docs/scEnrichment.ipynb)
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## Citation
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1. [Li, Mengwei, et al. "DISCO: a database of Deeply Integrated human Single-Cell Omics data." Nucleic acids research 50.D1 (2022): D596-D602.](https://academic.oup.com/nar/article/50/D1/D596/6430491)
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2. [Mengwei Li, Kok Siong Ang, Brian Teo, Uddamvathanak Rom, Minh N Nguyen, Sebastian Maurer-Stroh, Jinmiao Chen. "Rediscovering publicly available single-cell data with the DISCO platform." Nucleic Acids Research (2024): gkae1108](https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae1108/7899529)
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## Follow us on our social media!
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- [HSCRM2](https://twitter.com/HSCRM2)
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- [JinmiaoChenLab Github repo](https://github.com/JinmiaoChenLab)
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